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Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules.
Gillis, K D; Mossner, R; Neher, E.
Afiliação
  • Gillis KD; Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen, Federal Republic of Germany.
Neuron ; 16(6): 1209-20, 1996 Jun.
Article em En | MEDLINE | ID: mdl-8663997
We have used membrane capacitance measurements to assay Ca2+-triggered exocytosis in single bovine adrenal chromatin cells. Brief application of phorbol ester (PMA) enhances depolarization-evoked exocytosis severalfold while actually decreasing the Ca2+ current. Ca2+ metabolism is unchanged. Three different protocols were used to show that PMA increases the size of the readily releasable pool of secretory granules. PMA treatment leads to a large increase in amplitude, but little change in the time course of the exocytic burst that results from rapid elevation of [Ca2+]i upon photolysis of DMI-Nitrophen. Thus, PKC appears to affect a late step in secretion but not the Ca2+ sensitivity of the final step.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína Quinase C / Sistema Cromafim / Exocitose / Potenciais da Membrana Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 1996 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína Quinase C / Sistema Cromafim / Exocitose / Potenciais da Membrana Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 1996 Tipo de documento: Article