Immunohistochemical and immunoblotting identification of protein phosphatase 1 gamma 1 in rat salivary glands.
FEBS Lett
; 393(1): 57-9, 1996 Sep 09.
Article
em En
| MEDLINE
| ID: mdl-8804423
ABSTRACT
We have analyzed the distribution of the gamma 1 isotype of rat protein phosphatase type 1 catalytic subunit in rat salivary glands. Formaldehyde-fixed paraffin sections were reacted with the PP1 gamma 1 antibody using an immunohistochemical method. Positive staining occurred in striated ducts of parotid gland. However, the staining reaction was less intense in submandibular gland. Proteins were also prepared from rat salivary glands and subjected to SDS-PAGE, followed by Western blotting analysis with the PP1 gamma 1 antibody. The antibody interacted with protein corresponding to an estimated molecular mass of 36 kDa present in the parotid gland. The staining reaction was considerably weaker with the proteins from submandibular gland.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândulas Salivares
/
Fosfoproteínas Fosfatases
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão