Cloning of Human T and Natural Killer Cells
Methods
; 9(3): 416-21, 1996 Jun.
Article
em En
| MEDLINE
| ID: mdl-8812695
The discovery of the lymphokine interleukin 2 (IL-2), which induces growth of T cells, set the stage for establishing methods for in vitro cloning of human T cells. More recently, it has become clear that in addition to IL-2, other T-cell growth factors, such as IL-4 and IL-7, can be used for generation and expansion of T-cell clones. In the past it was shown that it is possible to grow T cells differing in function and phenotype. In vitro-expanded T-cell clones have been instrumental in studies on the antigen specificities and biological properties of these cells. Cloned lines of natural killer (NK) cells can also be established using IL-2, permitting detailed studies on the biology of these cells. Here we describe the methods we use to generate and to expand human T-cell and NK cell clones. We discuss the differences between IL-2 and IL-4 as growth factors to expand T-cell clones. In addition, we summarize some recent findings of studies using T- and NK cell clones.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Methods
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Holanda