A dominant mutation in the Chlamydomonas reinhardtii nuclear gene SIM30 suppresses translational defects caused by initiation codon mutations in chloroplast genes.
Genetics
; 145(4): 935-43, 1997 Apr.
Article
em En
| MEDLINE
| ID: mdl-9093848
ABSTRACT
A suppressor of a translation initiation defect caused by an AUG to AUU mutation in the Chlamydomonas reinhardtii chloroplast petD gene was isolated, defining a nuclear locus that we have named SIM30. A dominant mutant allele at this locus, sim30-1d, was found to increase the translation initiation rate of the mutant petD mRNA. sim30-1d was also able to suppress the translational defect caused by an AUG to AUC mutation in the petD gene, and an AUG to AUU mutation in the chloroplast petA gene. We therefore suggest that the SIM30 gene may encode a general chloroplast translation factor. The ability of sim30-1d to suppress the petD AUG to AUU mutation is diminished in the presence of one or more antibiotic resistance markers located within the 16S and 23S rRNAs, suggesting that the activity of the sim30-1d gene product in translation initiation may involve interaction with ribosomal subunits.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Códon
/
Proteínas de Protozoários
/
Cloroplastos
/
Regulação da Expressão Gênica
/
Genes Supressores
/
Chlamydomonas reinhardtii
/
Genes de Protozoários
/
Genes de Plantas
/
Complexo Citocromos b6f
Limite:
Animals
Idioma:
En
Revista:
Genetics
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos