Measurement of factor VII and of activated factor VII in healthy individuals and in prothrombin complex concentrates.
Thromb Res
; 86(6): 493-504, 1997 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-9219329
ABSTRACT
We established reference ranges for factor VII clotting activity (FVIIC), factor VII amidolytic activity (FVIIAM), and activated factor VII (FVIIa) in 102 healthy individuals. The reference ranges were 65-160 U/100 ml, 70-165 U/100 ml, and 30-170 mU/ml, respectively (2.5 and 97.5 percentiles). Freezing and thawing of the plasma samples had no influence on the assay results. Due to the small sample size, the results were not influenced by gender, age, smoking habits, and oral contraceptive use. The plasma levels of FVIIC, FVIIAM, and FVIIa were significantly correlated with each other. The significant correlation between FVIIa and FVIIAM indicates that FVIIa is not completely independent of circulating FVII mass. There was also a significant, though weak, correlation between FVIIa and FVIIC/FVIIAM ratios. Sixteen batches of prothrombin complex concentrates (PCC) from 3 manufacturers were also analysed. FVIIa could be detected in all preparations, with considerable variations from batch to batch. In contrast to the results obtained in plasma from normal individuals, there was a close correlation between FVIIa and FVIIC/FVIIAM ratios. The preparations could be characterized by their FVII and FVIIa potencies and by their FVIIC/FVIIAM ratios. In PCC, FVIIC was very strongly correlated with FVIIa, whereas no significant correlation was observed between FVIIAM and FVIIC and between FVIIAM and FVIIa, respectively. These results demonstrate that the FVIIC assay used is sensitive for detecting FVIIa. Thus, we cannot confirm that FVIIa sensitivity of one-stage clotting assays for FVIIC is low when a rabbit thromboplastin and a non-adsorbed FVII-deficient plasma is used.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator VII
/
Protrombina
/
Fator VIIa
Limite:
Adolescent
/
Adult
/
Animals
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Thromb Res
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Alemanha