Counteraction by MutT protein of transcriptional errors caused by oxidative damage.
Science
; 278(5335): 128-30, 1997 Oct 03.
Article
em En
| MEDLINE
| ID: mdl-9311918
ABSTRACT
Oxidized guanine (8-oxo-7,8-dihydroguanine; 8-oxo-G) is a potent mutagen because of its ambiguous pairing with cytosine and adenine. The Escherichia coli MutT protein specifically hydrolyzes both 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP) and 8-oxo-guanosine triphosphate (8-oxo-rGTP), which are otherwise incorporated in DNA and RNA opposite template A. In vivo, this cleaning of the nucleotide pools decreases both DNA replication and transcription errors. The effect of mutT mutation on transcription fidelity was shown to depend on oxidative metabolism. Such control of transcriptional fidelity by the ubiquitous MutT function has implications for evolution of RNA-based life, phenotypic expression, adaptive mutagenesis, and functional maintenance of nondividing cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Transcrição Gênica
/
RNA Mensageiro
/
Monoéster Fosfórico Hidrolases
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Guanosina Trifosfato
/
Mutação
Idioma:
En
Revista:
Science
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França