Structure of the Drosophila DNA topoisomerase I gene and expression of messages with different lengths in the 3' untranslated region.
Gene
; 211(2): 195-203, 1998 May 12.
Article
em En
| MEDLINE
| ID: mdl-9602125
ABSTRACT
The nucleotide sequence of the Drosophila DNA topoisomerase I gene (top1) has been determined. Structurally, top1 consists of eight exons and seven introns. The top1 coding region contains a new class of opa repeats, encoding clusters of serine residues instead of glutamine repeats usually seen in Drosophila genes of the neurogenic loci. A unique feature of top1 is the developmental switch of its transcripts a heterogeneous population of transcripts ranging from 3.8 to 4.2kb seen maximally at 0-2h of embryogenesis and a 5.2-kb transcript maximal at 6-12h of embryonic development. The transcripts expressed in the 0-2-h embryo have been shown as maternal storage products specific to ovarian tissues. RACE analysis shows that whereas the 6-12-h transcripts have a single site for polyadenylation, there are at least 12 different sites for poly(A) addition to the 0-2-h transcripts. An additional intron specific for the maternal storage transcripts appears in some of the 0-2-h transcripts. No significant heterogeneity at the 5' end of the top1 transcripts is seen. Sequence searches have revealed a number of regulatory sequences for potential translational control in the 3' untranslated region.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Genes de Insetos
/
DNA Topoisomerases Tipo I
/
Drosophila
Limite:
Animals
Idioma:
En
Revista:
Gene
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos