NMR identification of epitopes of Lyme disease antigen OspA to monoclonal antibodies.
J Mol Biol
; 281(1): 61-7, 1998 Aug 07.
Article
em En
| MEDLINE
| ID: mdl-9680475
ABSTRACT
Outer surface protein A (OspA) from the Lyme disease spirochete Borrelia burgdorferi has been a focus of vaccine development. We have identified epitopes of OspA to two monoclonal antibodies (mAbs) by comparing NMR chemical shifts of free OspA and those in Fab complexes. Deuteration of non-labile protons in OspA extended the size limit of this technique so that it was applicable to the 78 kDa complexes of OspA and the Fab fragment. The epitope identified by NMR to an mAb, 184.1, agrees well with that previously defined by the crystal structure of the same complex, indicating the ability of the NMR method to accurately map an epitope in a large protein complex. The technique mapped the epitope to mAb 336, a mAb of clinical interest, to a region centered at the C-terminal alpha-helix. The results provides a basis for rational design of OspA-based Lyme disease vaccines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Doença de Lyme
/
Espectroscopia de Ressonância Magnética
/
Grupo Borrelia Burgdorferi
/
Mapeamento de Epitopos
/
Lipoproteínas
/
Epitopos
/
Antígenos de Superfície
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos