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1.
Biofabrication ; 16(2)2024 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-38277671

RESUMO

Tissue engineering has emerged as a strategy for producing functional tissues and organs to treat diseases and injuries. Many chronic conditions directly or indirectly affect normal blood vessel functioning, necessary for material exchange and transport through the body and within tissue-engineered constructs. The interest in vascular tissue engineering is due to two reasons: (1) functional grafts can be used to replace diseased blood vessels, and (2) engineering effective vasculature within other engineered tissues enables connection with the host's circulatory system, supporting their survival. Among various practices, (bio)printing has emerged as a powerful tool to engineer biomimetic constructs. This has been made possible with precise control of cell deposition and matrix environment along with the advancements in biomaterials. (Bio)printing has been used for both engineering stand-alone vascular grafts as well as vasculature within engineered tissues for regenerative applications. In this review article, we discuss various conditions associated with blood vessels, the need for artificial blood vessels, the anatomy and physiology of different blood vessels, available 3D (bio)printing techniques to fabricate tissue-engineered vascular grafts and vasculature in scaffolds, and the comparison among the different techniques. We conclude our review with a brief discussion about future opportunities in the area of blood vessel tissue engineering.


Assuntos
Bioimpressão , Neovascularização Fisiológica , Neovascularização Fisiológica/fisiologia , Engenharia Tecidual/métodos , Materiais Biocompatíveis , Tecidos Suporte , Artérias , Impressão Tridimensional , Bioimpressão/métodos , Vasos Sanguíneos/fisiologia
2.
Bioessays ; 46(3): e2300091, 2024 03.
Artigo em Inglês | MEDLINE | ID: mdl-38135890

RESUMO

The sophisticated function of the central nervous system (CNS) is largely supported by proper interactions between neural cells and blood vessels. Accumulating evidence has demonstrated that neurons and glial cells support the formation of blood vessels, which in turn, act as migratory scaffolds for these cell types. Neural progenitors are also involved in the regulation of blood vessel formation. This mutual interaction between neural cells and blood vessels is elegantly controlled by several chemokines, growth factors, extracellular matrix, and adhesion molecules such as integrins. Recent research has revealed that newly migrating cell types along blood vessels repel other preexisting migrating cell types, causing them to detach from the blood vessels. In this review, we discuss vascular formation and cell migration, particularly during development. Moreover, we discuss how the crosstalk between blood vessels and neurons and glial cells could be related to neurodevelopmental disorders.


Assuntos
Sistema Nervoso Central , Neurônios , Neurônios/metabolismo , Sistema Nervoso Central/fisiologia , Movimento Celular/fisiologia , Integrinas/metabolismo , Vasos Sanguíneos/fisiologia
3.
J Virol ; 97(11): e0048023, 2023 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-37877715

RESUMO

IMPORTANCE: Viruses are able to mimic the physiological or pathological mechanism of the host to favor their infection and replication. Virus-mock basement membrane (VMBM) is a Megalocytivirus-induced extracellular structure formed on the surface of infected cells and structurally and functionally mimics the basement membrane of the host. VMBM provides specific support for lymphatic endothelial cells (LECs) rather than blood endothelial cells to adhere to the surface of infected cells, which constitutes a unique phenomenon of Megalocytivirus infection. Here, the structure of VMBM and the interactions between VMBM components and LECs have been analyzed at the molecular level. The regulatory effect of VMBM components on the proliferation and migration of LECs has also been explored. This study helps to understand the mechanism of LEC-specific attachment to VMBM and to address the issue of where the LECs come from in the context of Megalocytivirus infection.


Assuntos
Membrana Basal , Células Endoteliais , Iridoviridae , Vasos Linfáticos , Membrana Basal/metabolismo , Membrana Basal/virologia , Células Endoteliais/citologia , Células Endoteliais/imunologia , Células Endoteliais/metabolismo , Iridoviridae/fisiologia , Vasos Linfáticos/citologia , Proliferação de Células , Movimento Celular , Vasos Sanguíneos/citologia , Interações entre Hospedeiro e Microrganismos
4.
Angiol. (Barcelona) ; 75(5): 290-297, Sept-Oct, 2023. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-226583

RESUMO

Introducción y objetivo: la enfermedad oclusiva de las arterias ilíacas puede ser causa de complicaciones en EVAR. Su frecuencia no es muy alta, pero su mortalidad sí y hay evidencia escasa en cuanto a su repercusión en EVAR complejo. el uso de conductos iliofemorales es una herramienta que existe para combatir este problema. el objetivo de este trabajo es analizar el impacto del uso de conductos iliofemorales en la morbimortalidad neurológica y vascular en FeVaR y BeVaR. Materiales y métodos: recolección retrospectiva de pacientes con aneurismas yuxtarrenales, abdominotorácicos o endoleak ia tratados mediante FEVAR o BEVAR de forma electiva entre 2014 y 2020 en una sola institución (la Clínica La Sagrada Familia, Buenos aires, argentina). Se dividieron en dos grupos: uno, con conductos (grupo a), y otros, sin (grupo B). el grupo a se subdividió entre aquellos con conductos temporales y aquellos con permanentes. Resultados: analizamos 45 pacientes. 23 recibieron conductos (grupo a) y 22, no (grupo B). La edad media fue de 73 años y el diámetro promedio del saco fue de 69,89 mm. La estancia hospitalaria media fue de 4,7 días. el grupo a presentó más pacientes con enfermedad vascular periférica (56,5 % frente a 22,7 %, p = 0,045) y diámetros menores de arterias ilíacas externas. Hubo 8 complicaciones en el perioperatorio (17,8 %; grupo a, n = 1, 4,3 %, frente al grupo B, n = 7, 31,8 %; p = 0,043). Fallecieron 2 pacientes, lo que dejó una mortalidad perioperatoria del 4,4 % (grupo a, 0 %, frente al grupo B, 9,1 %; p = 0,45). Las complicaciones incluyeron isquemia medular, ruptura de la arteria ilíaca e isquemia de miembros inferiores. dentro del grupo a, 12 pacientes (52,2 %) recibieron conductos permanentes y otros 11 (47,8 %), temporales. Conclusiones: los conductos iliofemorales en FEVAR y BEVAR son seguros cuando forman parte de la planificación preoperatoria. Las complicaciones neurológicas y vasculares no son infrecuentes y conllevan una alta mortalidad...(AU)


Introduction and objective: occlusive arterial disease involving the iliac arteries can be cause of complicationsin eVaR. its frequency is not high, but its mortality is and there is scant evidence regarding its repercussion incomplex eVaR. the use of iliofemoral conduits is a tool to overcome this problem. our objective is to analyzethe impact of the use of iliofemoral conduits in the neurological and vascular morbimortality associated withFeVaR and BeVaR. Materials and methods: retrospective recollection of patients who underwent elective FeVaR or BeVaR for jux-tarrenal, thoracoabdominal aneurysms or type ia endoleak between 2014 and 2020 in one institution (Clínica LaSagrada Familia, Buenos aires, argentina). Patients were divided in two groups, one with conduits (group a) andone without (group B). Group a was subdivided between those who received temporary conduits and those withpermanent conduits. Results: we analyzed 45 patients. 23 received conduits (group a) whereas 22 did not (group B). mean age was 73years and mean sac diameter was 69.89 mm. mean hospital stay was 4.7 days. Group a presented more patientswith peripheral vascular disease (56.5 % vs. 22.7 %, p = 0.045) and smaller iliac arteries. there were 8 complicationsin the perioperative period (17.8 %; group a, n = 1, 4.3 %; group B, n = 7, 31.8 %. p = 0.043). 2 patients died, leavinga perioperative mortality of 4.4 % (group a 0 % vs. group B 9.1 %, p = 0.45). Complications included spinal cordischemia, iliac artery rupture and lower limb ischemia. in group a, 12 (52.2 %) patients received permanent conduitsand 11 (47.8 %) temporary conduits. Conclusions: the use of iliofemoral conduits in FeVaR and BeVaR is safe when they are part of the preoperativeplanning. neurological and vascular complications are not infrequent and carry a high mortality. the use of conduitsis effective to reduce its incidence and associated mortality.(AU)


Assuntos
Humanos , Masculino , Feminino , Idoso , Artéria Ilíaca/cirurgia , Artéria Ilíaca/anatomia & histologia , Aneurisma Aórtico/complicações , Aneurisma Aórtico/cirurgia , Procedimentos Endovasculares , Argentina , Estudos Retrospectivos , Indicadores de Morbimortalidade , Sistema Linfático , Vasos Sanguíneos , Sistema Cardiovascular
5.
Rinsho Ketsueki ; 64(7): 661-664, 2023.
Artigo em Japonês | MEDLINE | ID: mdl-37544727

RESUMO

By carrying a systemic circulation, hematopoietic and vascular systems coordinately govern the functional organ connections in the body. Blood vessels play an important role in the development, regeneration, and maintenance of organs by acting as conduits for environmental factors in the blood to tissues and secreting organ-specific cytokines as angiocrine signals. Recently, it has become clear that vascular endothelial cells, which are the main constituent cells of the blood vessels and play a role in homeostasis, are diverse. It has also been established that the cells of stem cell fraction exist in endothelial cells. The vascular endothelial cells in various organs are functionally different. For example, it has been discovered that sinusoidal blood vessels in the liver produce coagulation factor VIII as an organ-specific vascular function. Determining how such tissue-/organ-specific function of the endothelial cells is induced is a topic of interest in the vascular field of study.


Assuntos
Capilares , Células Endoteliais , Hemofilia A , Fígado , Humanos , Fígado/irrigação sanguínea , Fígado/fisiologia , Vasos Sanguíneos , Capilares/fisiologia
6.
Actas dermo-sifiliogr. (Ed. impr.) ; 114(7): 606-612, jul.- ago. 2023. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-223003

RESUMO

En el presente artículo de la serie «Seguridad en procedimientos dermatológicos» se aborda la sección quirúrgica accidental de grandes vasos sanguíneos y estructuras nerviosas. Se aborda, en primer lugar, la localización anatómica y recorrido de las distintas estructuras vasculares y nerviosas de más riesgo. A continuación, se explican las consecuencias de dicha lesión. Por último, se emiten algunas recomendaciones para evitar el daño accidental de las estructuras en dichas áreas de riesgo y se plantean algunas maniobras terapéuticas de reparación ante un eventual daño (AU)


This article in the series «Safety in Dermatologic Procedures» deals with the accidental laceration of major blood vessels and nerve structures during surgery. We first look at the anatomic location and course of the blood vessels and nerve structures that are most at risk of injury and then describe the possible outcomes in each case. We finally offer some recommendations on how to avoid damage to structures in danger zones and how to repair them if they are accidentally compromised (AU)


Assuntos
Humanos , Vasos Sanguíneos/lesões , Traumatismos dos Nervos Periféricos/etiologia , Fatores de Risco
7.
Actas dermo-sifiliogr. (Ed. impr.) ; 114(7): t606-t612, jul.- ago. 2023. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-223004

RESUMO

This article in the series «Safety in Dermatologic Procedures» deals with the accidental laceration of major blood vessels and nerve structures during surgery. We first look at the anatomic location and course of the blood vessels and nerve structures that are most at risk of injury and then describe the possible outcomes in each case. We finally offer some recommendations on how to avoid damage to structures in danger zones and how to repair them if they are accidentally compromised (AU)


En el presente artículo de la serie «Seguridad en procedimientos dermatológicos» se aborda la sección quirúrgica accidental de grandes vasos sanguíneos y estructuras nerviosas. Se aborda, en primer lugar, la localización anatómica y recorrido de las distintas estructuras vasculares y nerviosas de más riesgo. A continuación, se explican las consecuencias de dicha lesión. Por último, se emiten algunas recomendaciones para evitar el daño accidental de las estructuras en dichas áreas de riesgo y se plantean algunas maniobras terapéuticas de reparación ante un eventual daño (AU)


Assuntos
Humanos , Vasos Sanguíneos/lesões , Traumatismos dos Nervos Periféricos/etiologia , Fatores de Risco
10.
Biofabrication ; 15(4)2023 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-37487489

RESUMO

Organ transplantation is a definitive treatment for endocrine disorders, but donor shortages limit the use of this technique. The development of regenerative therapies would revolutionize the treatment of endocrine disorders. As is the case for harvested organs, the ideal bioengineered graft would comprise vascularized endocrine tissue, contain blood vessels that could be anastomosed to host vessels, have stable blood flow, and be suitable for transplantation into various sites. Here, we describe a transplantable endocrine tissue graft that was fabricated byex vivoperfusion of tricultured cell sheets (isletß-cells, vascular endothelial cells (vECs), and mesenchymal stem cells (MSCs)) on a vascularized tissue flap ofin vivoorigin. The present study has three key findings. First, mild hypothermic conditions enhanced the success ofex vivoperfusion culture. Specifically, graft construction failed at 37 °C but succeeded at 32 °C (mild hypothermia), and endocrine tissue fabricated under mild hypothermia contained aggregations of isletß-cells surrounded by dense vascular networks. Second, the construction of transplantable endocrine tissue byex vivoperfusion culture was better achieved using a vascular flap (VF) than a muscle flap. Third, the endocrine tissue construct generated using a VF could be transplanted into the rat by anastomosis of the graft artery and vein to host blood vessels, and the graft secreted insulin into the host's circulatory system for at least two weeks after transplantation. Endocrine tissues bioengineered using these techniques potentially could be used as novel endocrine therapies.


Assuntos
Hipotermia , Engenharia Tecidual , Ratos , Animais , Engenharia Tecidual/métodos , Células Endoteliais , Bioengenharia , Vasos Sanguíneos
11.
Am J Physiol Heart Circ Physiol ; 325(1): H172-H186, 2023 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-37294893

RESUMO

The adipokine chemerin may support blood pressure, evidenced by a fall in mean arterial pressure after whole body antisense oligonucleotide (ASO)-mediated knockdown of chemerin protein in rat models of normal and elevated blood pressure. Although the liver is the greatest contributor of circulating chemerin, liver-specific ASOs that abolished hepatic-derived chemerin did not change blood pressure. Thus, other sites must produce the chemerin that supports blood pressure. We hypothesize that the vasculature is a source of chemerin independent of the liver that supports arterial tone. RNAScope, PCR, Western blot analyses, ASOs, isometric contractility, and radiotelemetry were used in the Dahl salt-sensitive (SS) rat (male and female) on a normal diet. Retinoic acid receptor responder 2 (Rarres2) mRNA was detected in the smooth muscle, adventitia, and perivascular adipose tissue of the thoracic aorta. Chemerin protein was detected immunohistochemically in the endothelium, smooth muscle cells, adventitia, and perivascular adipose tissue. Chemerin colocalized with the vascular smooth muscle marker α-actin and the adipocyte marker perilipin. Importantly, chemerin protein in the thoracic aorta was not reduced when liver-derived chemerin was abolished by a liver-specific ASO against chemerin. Chemerin protein was similarly absent in arteries from a newly created global chemerin knockout in Dahl SS rats. Inhibition of the receptor Chemerin1 by the receptor antagonist CCX832 resulted in the loss of vascular tone that supports potential contributions of chemerin by both perivascular adipose tissue and the media. These data suggest that vessel-derived chemerin may support vascular tone locally through constitutive activation of Chemerin1. This posits chemerin as a potential therapeutic target in blood pressure regulation.NEW & NOTEWORTHY Vascular tunicas synthesizing chemerin is a new finding. Vascular chemerin is independent of hepatic-derived chemerin. Vasculature from both males and females have resident chemerin. Chemerin1 receptor activity supports vascular tone.


Assuntos
Vasos Sanguíneos , Quimiocinas , Animais , Ratos , Técnicas de Silenciamento de Genes , Fígado/metabolismo , Aorta/metabolismo , Quimiocinas/análise , Quimiocinas/metabolismo , Músculo Liso Vascular/metabolismo , Vasos Sanguíneos/metabolismo , Vasos Sanguíneos/patologia
12.
Angiol. (Barcelona) ; 75(3): 125-135, May-Jun. 2023. tab, graf, ilus
Artigo em Espanhol | IBECS | ID: ibc-221634

RESUMO

Introducción: la enfermedad arterial periférica (EAP) se considera una patología infradiagnosticada que afecta a cerca de doscientos millones de personas y se asocia con una alta carga de morbimortalidad. En muchos casos, se prefiere el manejo endovascular sobre el quirúrgico abierto, especialmente en pacientes con múltiples comorbilidades, que limitan este último abordaje. Sin embargo, presenta limitaciones relacionadas con el uso de medio de contraste en pacientes que adicionalmente presentan múltiples comorbilidades, incluyendo enfermedad renal, que podría comprometer su patología de base. Por esto, surge la necesidad de implementar un método que limite el uso de contraste sin comprometer el éxito técnico del procedimiento. Objetivo: describir las características de las lesiones arteriales, los resultados posquirúrgicos y las complicaciones de los pacientes que recibieron manejo de la EAP vía endovascular bajo guía ultrasonográfica. Materiales y métodos: se realizó un análisis retrospectivo de los pacientes que recibieron tratamiento endovascular bajo guía ultrasonográfica para el tratamiento de EAP con amenaza de la extremidad y su resultado posoperatorio durante el periodo comprendido entre enero de 2018 y enero de 2022 en el Hospital Universitario Samaritana (Bogotá, Colombia). Resultados: se identificaron un total de 40 pacientes con 3 tipos de lesiones, incluyendo estenosis, oclusiones y lesiones mixtas, que fueron intervenidas con angioplastia con balón o con stent o trombectomías, todos bajo guía ultrasonográfica. La principal complicación asociada fue el pseudoaneurisma posterior a la retirada del catéter arterial en el 22,5 % de las ocasiones, seguida de la disección arterial, en el 13 % de las veces. Se consideró éxito posquirúrgico en el 92,7 % de los pacientes, evidenciado por mejoría en el porcentaje de velocidad pico sistólica final o en la morfología de la...(AU)


Introduction: peripheral arterial disease (PAD) is considered an underdiagnosed pathology that affects nearly 200 million people and is associated with a high burden of morbidity and mortality. In many cases, endovascular management is preferred over open surgery, especially in patients with multiple comorbidities that limit the latter approach. However, it has limitations related to the use of contrast medium in the context of patients who addi- tionally have multiple comorbidities, including kidney disease, which can compromise their underlying pathology. Therefore, the need arises to implement a method that limits the use of contrast, without compromising the technical success of the procedure. Materials and methods: a retrospective analysis of those patients who received endovascular treatment under ultrasonography guidance for the treatment of acute limb ischemia and their postoperative outcome. Objective: describe the characteristics of the arterial lesions, the post-surgical results and the complications of the patients who received management of arterial occlusive disease with extremity threat by endovascular approach under ultrasonographic guidance, at the University Hospital La Samaritana (Bogotá, Colombia) between 2018 - 2022. Results: a total of 40 patients were identified with 3 types of lesions including stenosis, and mixed lesions. Those patients received either balloon angioplasty, angioplasty with stent or thrombectomies. The main associated complication was pseudoaneurysm after arterial catheter removal in 22.5 %, followed by arterial dissection in 13 %. Post-surgical success was considered in 92.7 % of patients, evidenced either on the improvement in the percentage of final peak systolic velocity or in the morphology of the artery distal to the intervened segment.Conclusion: The use of ultrasonography as a guide for endovascular procedures is presented as an alternative to conventional angiography, with good success rates.(AU)


Assuntos
Humanos , Doença Arterial Periférica/diagnóstico , Doença Arterial Periférica/tratamento farmacológico , Procedimentos Endovasculares , Trombectomia , Angioplastia , Colômbia , Estudos Retrospectivos , Epidemiologia Descritiva , Sistema Cardiovascular , Vasos Sanguíneos , Ultrassonografia
13.
Am J Physiol Renal Physiol ; 325(1): F22-F37, 2023 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-37167273

RESUMO

Increased mechanical endothelial cell stretch contributes to the development of numerous cardiovascular and renal pathologies. Recent studies have shone a light on the importance of sex-dependent inflammation in the pathogenesis of renal disease states. The endothelium plays an intimate and critical role in the orchestration of immune cell activation through upregulation of adhesion molecules and secretion of cytokines and chemokines. While endothelial cells are not recognized as professional antigen-presenting cells, in response to cytokine stimulation, endothelial cells can express both major histocompatibility complex (MHC) I and MHC II. MHCs are essential to forming a part of the immunological synapse interface during antigen presentation to adaptive immune cells. Whether MHC I and II are increased under increased mechanical stretch is unknown. Due to hypertension being multifactorial, we hypothesized that increased mechanical endothelial stretch promotes the regulation of MHCs and key costimulatory proteins on mouse renal endothelial cells (MRECs) in a stretch-dependent manner. MRECs derived from both sexes underwent 5%, 10%, or 15% uniaxial cyclical stretch, and immunological synapse interface proteins were determined by immunofluorescence microscopy, immunoblot analysis, and RNA sequencing. We found that increased endothelial mechanical stretch conditions promoted downregulation of MHC I in male MRECs but upregulation in female MRECs. Moreover, MHC II was upregulated by mechanical stretch in both male and female MRECs, whereas CD86 and CD70 were regulated in a sex-dependent manner. By bulk RNA sequencing, we found that increased mechanical endothelial cell stretch promoted differential gene expression of key antigen processing and presentation genes in female MRECs, demonstrating that females have upregulation of key antigen presentation pathways. Taken together, our data demonstrate that mechanical endothelial stretch regulates endothelial activation and immunological synapse interface formation in renal endothelial cells in a sex-dependent manner.NEW & NOTEWORTHY Endothelial cells contribute to the development of renal inflammation and have the unique ability to express antigen presentation proteins. Whether increased endothelial mechanical stretch regulates immunological synapse interface proteins remains unknown. We found that antigen presentation proteins and costimulatory proteins on renal endothelial cells are modulated by mechanical stretch in a sex-dependent manner. Our data provide novel insights into the sex-dependent ability of renal endothelial cells to present antigens in response to endothelial mechanical stimuli.


Assuntos
Vasos Sanguíneos , Células Endoteliais , Sinapses Imunológicas , Rim , Células Endoteliais/fisiologia , Células Cultivadas , Masculino , Feminino , Animais , Camundongos , Rim/irrigação sanguínea , Camundongos Endogâmicos C57BL , Vasos Sanguíneos/citologia , Fenômenos Biomecânicos , Inflamação/metabolismo , Secretoma/metabolismo , Caracteres Sexuais , Complexo Principal de Histocompatibilidade , Antígeno B7-2/metabolismo , Apresentação de Antígeno
14.
J Biomed Opt ; 28(9): 094807, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37234194

RESUMO

Significance: Optical imaging in the second near-infrared (NIR-II, 1000 to 1700 nm) region is capable of deep tumor vascular imaging due to low light scattering and low autofluorescence. Non-invasive real-time NIR-II fluorescence imaging is instrumental in monitoring tumor status. Aim: Our aim is to develop an NIR-II fluorescence rotational stereo imaging system for 360-deg three-dimensional (3D) imaging of whole-body blood vessels, tumor vessels, and 3D contour of mice. Approach: Our study combined an NIR-II camera with a 360-deg rotational stereovision technique for tumor vascular imaging and 3D surface contour for mice. Moreover, self-made NIR-II fluorescent polymer dots were applied in high-contrast NIR-II vascular imaging, along with a 3D blood vessel enhancement algorithm for acquiring high-resolution 3D blood vessel images. The system was validated with a custom-made 3D printing phantom and in vivo experiments of 4T1 tumor-bearing mice. Results: The results showed that the NIR-II 3D 360-deg tumor blood vessels and mice contour could be reconstructed with 0.15 mm spatial resolution, 0.3 mm depth resolution, and 5 mm imaging depth in an ex vivo experiment. Conclusions: The pioneering development of an NIR-II 3D 360-deg rotational stereo imaging system was first applied in small animal tumor blood vessel imaging and 3D surface contour imaging, demonstrating its capability of reconstructing tumor blood vessels and mice contour. Therefore, the 3D imaging system can be instrumental in monitoring tumor therapy effects.


Assuntos
Neoplasias , Animais , Camundongos , Neoplasias/diagnóstico por imagem , Neoplasias/irrigação sanguínea , Imagem Óptica/métodos , Corantes , Imageamento Tridimensional/métodos , Vasos Sanguíneos
15.
Angiol. (Barcelona) ; 75(2): 78-84, Mar-Abr. 2023. tab
Artigo em Espanhol | IBECS | ID: ibc-219057

RESUMO

La isquemia intestinal asocia una elevada mortalidad debida principalmente a un retraso en el diagnóstico. Sibien el angio tC es una herramienta sensible y específica, suele transcurrir demasiado tiempo hasta su realizacióndebido a una presentación clínica poco específica. en este tiempo la isquemia intestinal puede progresar a estadiosirreversibles con afectación sistémica. La obtención de biomarcadores precisos y de elevación precoz acortaría eltiempo diagnóstico de esta patología, lo que disminuiría su mortalidad asociada. Se sabe que las moléculas usadastradicionalmente, entre ellas el lactato, no tienen buena capacidad diagnóstica. no obstante, se ha observado unaelevada sensibilidad con el uso del esteroisómero D del lactato y la procalcitonina para detectar colitis isquémicatras cirugía de aorta, al tiempo que se recomienda valorar los niveles de dímero D para descartar isquemia mesen-térica aguda en pacientes con dolor abdominal. Otras moléculas con un potencial rendimiento diagnóstico sonla proteína ligadora de ácidos grasos intestinales (I-FaBp) y el péptido similar al glucagón de tipo 1 (GLp-1), aúnen investigación.(AU)


Intestinal ischemia associates high mortality rates, mainly due to a delay in diagnosis. although computed tomog-raphy angiography (Cta) remains a sensitive and specifi c tool, it usually takes quite long until it is done, due to anunspecific clinical presentation. In this time lapse, intestinal ischemia may progress to an irreversible stage withsigns of systemic failure. the acquisition of precise and early detection biomarkers for the disease would shortenthe time to diagnosis and hence its associated mortality. It is acknowledged that those molecules which have beenclassically used-lactate amongst them-do not have a proper diagnostic capacity. nevertheless, the D stereoisomerof lactate and procalcitonin have shown high sensitivity for detection of ischemic colitis after open aortic surgery,while D-dimer measurement is recommended to rule out acute mesenteric ischemia in patients with abdominalpain. Other molecules with a potential for diagnostic yield are intestinal fatty acid binding protein (i-FaBp) andglucagon-like peptide-1 (GLp-1), still under investigation.(AU)


Assuntos
Humanos , Biomarcadores , Mortalidade , Intestinos/lesões , Colite Isquêmica , Isquemia Mesentérica , Doenças Cardiovasculares , Vasos Sanguíneos
16.
Angiol. (Barcelona) ; 75(2): 97-100, Mar-Abr. 2023. ilus
Artigo em Espanhol | IBECS | ID: ibc-219059

RESUMO

La revascularización distal de las extremidades inferiores siempre fue motivo de controversia en la década de losaños ochenta del siglo pasado entre los que defendían el bypass de vena safena invertida frente al bypass de venasafena in situ, aunque los resultados, como se demostró posteriormente, fueron similares. Lo importante era elmaterial sustitutivo (la vena autóloga) y la técnica de revascularización del cirujano. el bypass in situ permitía una disposición anatómica sin generar disparidad de calibres en la parte proximal y distalde las anastomosis vasculares, lo que evitaba el riesgo de torsiones. es cierto que comportaba riesgos secundarios,como la persistencia de fístulas arteriovenosas y la necesidad inherente de rasgar las válvulas venosas. Diversosartilugios y métodos se usaron a lo largo del tiempo, pero finalmente el valvulotomo de Lemaitre se estableciócomo uno de los más seguros. Los autores de este artículo describen cómo lo hacen y señalan los puntos críticospara optimizar el resultado, si bien son conscientes de que habrá variantes entre grupos quirúrgicos, pero mante-niendo la esencia del procedimiento.(AU)


Distal revascularization of the lower extremities was always a source of controversy in the 1980s among thosewho advocated reverse saphenous vein bypass versus in situ saphenous vein bypass, although the late resultsdemonstrated that both approaches were similar. the important fact was the substitute material (the autologousvein) and the revascularization technique of the vascular surgeon.the in-situ bypass allowed an anatomical position without produces caliber disparity in the proximal and distalvascular anastomoses, avoiding the risk of twisting. It is true that it carried secondary risks such as the persistenceof arteriovenous fistulas and the inherent need to tear the venous valves. Various methods were used over time,but eventually Lemaitre's valvulotome established itself as one of the safest. the authors of this article describehow they do it and point out the critical points to optimize the result, although they are aware that there will bevariations between surgical groups, but maintaining the essence of the procedure.(AU)


Assuntos
Humanos , Veia Safena , Extremidade Inferior , Vasos Sanguíneos , Procedimentos Cirúrgicos Vasculares
20.
Sci Rep ; 13(1): 4650, 2023 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-36944724

RESUMO

Blood flow reflects the eye's health and is disrupted in many diseases. Many pathological processes take place at the cellular level like as microcirculation of blood in vessels, and the processing of medical images is a difficult recognition task. Existing techniques for measuring blood flow are limited due to the complex assumptions, equipment and calculations requirements. In this paper, we propose a method for determining the blood flow characteristics in eye conjunctiva vessels, such as linear and volumetric blood speed and topological characteristics of the vascular net. The method preprocesses the video to improve the conditions of analysis and then builds an integral optical flow for definition of flow dynamical characteristic of eye vessels. These characteristics make it possible to determine changes in blood flow in eye vessels. We show the efficiency of our method in natural eye vessel scenes. The research provides valuable insights to novices with limited experience in the diagnosis and can serve as a valuable tool for experienced medical professionals.


Assuntos
Hemodinâmica , Esclera , Microcirculação , Algoritmos , Automação , Vasos Sanguíneos/fisiologia
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