Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 6.706
Filtrar
1.
Methods Mol Biol ; 2756: 351-382, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38427305

RESUMO

The growing interest in the use of entomopathogenic nematodes and their symbiotic bacteria as promising biocontrol agents of many arthropod pests and pathogens has created running technologies to expand their use globally. The related laboratory procedures and tests on these nematodes such as their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance should form the solid basis for such an expansion with reliable uses. Extensive practical details of such procedures and tests as well as how to identify and overcome the problems associated with these aspects are addressed in this chapter.


Assuntos
Artrópodes , Nematoides , Animais , Controle Biológico de Vetores/métodos , Reprodução , Simbiose
2.
Proc Biol Sci ; 291(2018): 20232937, 2024 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-38471545

RESUMO

Collembola is a highly diverse and abundant group of soil arthropods with chromosome numbers ranging from 5 to 11. Previous karyotype studies indicated that the Tomoceridae family possesses an exceptionally long chromosome. To better understand chromosome size evolution in Collembola, we obtained a chromosome-level genome of Yoshiicerus persimilis with a size of 334.44 Mb and BUSCO completeness of 97.0% (n = 1013). Both genomes of Y. persimilis and Tomocerus qinae (recently published) have an exceptionally large chromosome (ElChr greater than 100 Mb), accounting for nearly one-third of the genome. Comparative genomic analyses suggest that chromosomal elongation occurred independently in the two species approximately 10 million years ago, rather than in the ancestor of the Tomoceridae family. The ElChr elongation was caused by large tandem and segmental duplications, as well as transposon proliferation, with genes in these regions experiencing weaker purifying selection (higher dN/dS) than conserved regions. Moreover, inter-genomic synteny analyses indicated that chromosomal fission/fusion events played a crucial role in the evolution of chromosome numbers (ranging from 5 to 7) within Entomobryomorpha. This study provides a valuable resource for investigating the chromosome evolution of Collembola.


Assuntos
Artrópodes , Genoma , Animais , Artrópodes/genética , Genômica , Sintenia , Cariótipo , Evolução Molecular
3.
Zootaxa ; 5406(4): 588-600, 2024 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-38480126

RESUMO

Based on the review of the original descriptions of Pachymerium antipai Cpue, 1968, P. atticum Verhoeff, 1901 and P. tabacarui Cpue, 1968 from Romania and the direct study of the external anatomy of P. ferrugineum (C. L. Koch, 1835) and Geophilus flavus (De Geer, 1778) from Romania, Egypt, Italy and Russia, P. antipai and P. aticum sensu Cpue (1968) are proposed as junior subjective synonyms of P. ferrugineum, while P. tabacarui is proposed as a junior subjective synonym of G. flavus. The presence of P. atticum in Romania is hereby considered doubtful. Revisions to the diagnosis of Pachymerium are proposed to help differentiate it from other geophilid genera. A discussion of the relative suitability of different morphological characters for distinguishing species within Pachymerium is also included.


Assuntos
Artrópodes , Quilópodes , Animais , Romênia
4.
Zootaxa ; 5415(1): 56-76, 2024 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-38480215

RESUMO

The paradoxosomatid genus Iulidesmus Silvestri, 1895 in the fauna of Colombia is shown to presently contain nine species. Three of them are still obscure, two of which could be revised based on type (I. alacer (Attems, 1944)) or new material (Orthomorpha proxima Silvestri, 1898), one is a nomen dubium (Strongylosoma glabrum Peters, 1864), while further six are described as new here: Iulidesmus garciae n. sp., I. minerus n. sp., I. florezi n. sp., I. sabaneta n. sp., I. icni n. sp., and I. jeekeli n. sp. A key to all six new species of the genus and their distribution map are provided.


Assuntos
Artrópodes , Animais , Colômbia
5.
Zootaxa ; 5419(4): 545-562, 2024 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-38480309

RESUMO

Thailand hosts a very rich but underexplored giant pill-millipede (Sphaerotheriida) fauna, with 11 of its 13 species described in the last three years. Currently, all known Thai giant pill-millipedes belong to the genera Zephronia Gray, 1832 (nine species) and Sphaerobelum Verhoeff, 1924 (four species). Here we describe the first two species of the genus Prionobelum Verhoeff, 1924 (previously restricted to Vietnam and China), Prionobelum inthanonense n. sp. and P. naevium n. sp. from Thailand. The species occur at Thailands highest mountain (2500 m) Doi Inthanon and the lowland rainforests at Bang Lang National Park touching the border with Malaysia. Both species are described integratively, utilizing light microscopy, scanning electron microscopy as well as DNA barcoding. Both new species of Prionobelum differ from other Zephroniidae species, as well as from one another, by more than 20% p-distance in the COI barcoding gene suggesting that potential closer related species are still awaiting discovery.


Assuntos
Artrópodes , Animais , Tailândia , Artrópodes/genética , Microscopia Eletrônica de Varredura
6.
Zootaxa ; 5419(3): 401-418, 2024 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-38480317

RESUMO

Schendyla antici sp. nov., a dwarf geophilomorph from the Mt. Medvednik (Western Serbia, Balkan Peninsula), is described and illustrated based on the specimens extracted from the soil samples. A detailed comparison with all species within the genus is provided. The new species has the lowest number of leg-bearing segments within the genus Schendyla Bergse & Meinert, 1866, and one of the lowest in the order Geophilomorpha in general.


Assuntos
Artrópodes , Quilópodes , Animais , Península Balcânica , Sérvia
7.
Zootaxa ; 5405(2): 281-295, 2024 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-38480384

RESUMO

A new species of Alloscopus Brner, 1906 (Orchesellidae: Heteromurinae), A. arborealis sp. nov., is herein described from Mt. Makiling, Laguna, Philippines. The new species is distinct from its blind congeners by the combination of: 7+7 macrochaetae on head An series and 1+1 on M series; 13+13 macrochaetae on thorax II (vs. 912) and 7+7 central on thorax III (vs. 6+6); absence of the microsensilla on abdomen I; abdomen IV with four sensilla (vs. three); and ventral tube with a small number of chaetae on its posterior face (45 vs. 1123) and lateral flap (7 vs. 916). The complete body chaetotaxic pattern of the new species and a revised key to the world fauna of Alloscopus are also provided.


Assuntos
Artrópodes , Animais , Filipinas
9.
Molecules ; 29(5)2024 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-38474459

RESUMO

The essential oils and aroma derived from the leaves (L), stems (St), and spikes (s) of Piper nigrum L. cv. Guajarina were extracted; the essential oils were extracted using hydrodistillation (HD), and steam distillation (SD), and the aroma was obtained by simultaneous distillation and extraction (SDE). Chemical constituents were identified and quantified using GC/MS and GC-FID. Preliminary biological activity was assessed by determining the toxicity against Artemia salina Leach larvae, calculating mortality rates, and determining lethal concentration values (LC50). The predominant compounds in essential oil samples included α-pinene (0-5.6%), ß-pinene (0-22.7%), limonene (0-19.3%), 35 linalool (0-5.3%), δ-elemene (0-10.1%), ß-caryophyllene (0.5-21.9%), γ-elemene (7.5-33.9%), and curzerene (6.9-31.7%). Multivariate analysis, employing principal component analysis (PCA) and hierarchical cluster analysis (HCA), revealed three groups among the identified classes and two groups among individual compounds. The highest antioxidant activity was found for essential oils derived from the leaves (167.9 41 mg TE mL-1). Larvicidal potential against A. salina was observed in essential oils obtained from the leaves (LC50 6.40 µg mL-1) and spikes (LC50 6.44 µg mL-1). The in silico studies demonstrated that the main compounds can interact with acetylcholinesterase, thus showing the potential molecular interaction responsible for the toxicity of the essential oil in A. salina.


Assuntos
Artrópodes , Óleos Voláteis , Piper nigrum , Piper , Sesquiterpenos , Animais , Óleos Voláteis/química , Acetilcolinesterase , Cromatografia Gasosa-Espectrometria de Massas , Piper/química , Óleos de Plantas/química
10.
J Gen Virol ; 105(3)2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38441560

RESUMO

Plant viruses are transmitted mechanically or by vegetative propagation, and by vectors such as arthropods, fungi, nematodes, or parasitic plants. Sources to access available information regarding plant virus transmissions are scattered and require extensive literature searches. Here, a recently created plant virus transmission database is described. This was developed to provide access to the modes of transmission and vectors of over 1600 plant viruses. The database was compiled using over 3500 publication records spanning the last 100 years. The information is publicly accessible via https://library.wur.nl/WebQuery/virus and fully searchable by virus name, taxonomic position, mode of transmission or vector.


Assuntos
Artrópodes , Vírus de Plantas , Animais , Vírus de Plantas/genética , Bases de Dados Factuais
11.
Sci Total Environ ; 922: 171409, 2024 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-38432367

RESUMO

Arsenic (As) is a widespread metalloid with well-known toxicity. To date, numerous studies have focused on individual level toxicity (e.g., growth and reproduction) of As to typical invertebrate springtails in soils, however, the molecular level toxicity and mechanism was poorly understood. Here, an integrated transcriptomics and metabolomics approach was used to reveal responses of Folsomia candida exposed to As(V) of 10 and 60 mg kg-1 at which the individual level endpoints were influenced. Transcriptomics identified 5349 and 4020 differentially expressed genes (DEGs) in low and high concentration groups, respectively, and the most DEGs were down-regulated. Enrichment analysis showed that low and high concentrations of As(V) significantly inhibited chromatin/chromosome-related biological processes (chromatin/chromosome organization, nucleosome assembly and organization, etc.) in springtails. At high concentration treatment, structural constituent of cuticle, chitin metabolic process and peptidase activity (serine-type peptidase activity, endopeptidase activity, etc.) were inhibited or disturbed. Moreover, the apoptosis pathway was significantly induced. Metabolomics analysis identified 271 differential changed metabolites (DCMs) in springtails exposed to high concentration of As. Steroid hormone biosynthesis was the most significantly affected pathway. Several DCMs that related to chitin metabolism could further support above transcriptomic results. These findings further extended the knowledge of As toxic mechanisms to soil fauna and offer important information for the environmental risk assessment.


Assuntos
Arsênio , Artrópodes , Poluentes do Solo , Animais , Arsênio/metabolismo , Poluentes do Solo/metabolismo , Perfilação da Expressão Gênica , Metabolômica , Cromatina/metabolismo , Peptídeo Hidrolases/metabolismo , Quitina/metabolismo , Solo/química
12.
Nat Commun ; 15(1): 2117, 2024 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-38459063

RESUMO

Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account for a subset of existing arthropod species in nature. Here we use and develop advanced techniques to describe immune cells (hemocytes) from the clinically relevant tick Ixodes scapularis at a single-cell resolution. We observe molecular alterations in hemocytes upon feeding and infection with either the Lyme disease spirochete Borrelia burgdorferi or the rickettsial agent Anaplasma phagocytophilum. We reveal hemocyte clusters exhibiting defined signatures related to immunity, metabolism, and proliferation. Depletion of phagocytic hemocytes affects hemocytin and astakine levels, two I. scapularis hemocyte markers, impacting blood-feeding, molting behavior, and bacterial acquisition. Mechanistically, astakine alters hemocyte proliferation, whereas hemocytin affects the c-Jun N-terminal kinase (JNK) signaling pathway in I. scapularis. Altogether, we discover a role for tick hemocytes in immunophysiology and provide a valuable resource for comparative biology in arthropods.


Assuntos
Anaplasma phagocytophilum , Artrópodes , Borrelia burgdorferi , Ixodes , Doença de Lyme , Animais , Hemócitos , Ixodes/microbiologia , Borrelia burgdorferi/fisiologia
13.
Pak J Biol Sci ; 27(2): 100-107, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38516751

RESUMO

<b>Background and Objective:</b> The rapid development of Kendari City as the capital of Southeast Sulawesi Province has led to changes in land use patterns, particularly an increase in built-up areas, which threaten environmental stability. Rapid population growth contributes to rising carbon dioxide emissions, impacting air quality. Green spaces like Baruga Forest are ideal for environmental balance and bioservation. This study aims to identify the diversity of Collembola species in Baruga Forest, Kendari and understand the environmental factors influencing their presence. <b>Materials and Methods:</b> Collembola was collected in the Baruga Forest using a Berlese funnel, then identified and classified based on the family by counting the number of individuals in each taxonomic group. The diversity of Collembola was measured using the Shannon-Wiener index, while the evenness of individual distribution in each family was assessed using an evenness index. Additionally, environmental parameters such as air temperature, air humidity and soil pH were measured. The collected data were analyzed using descriptive statistical analysis. <b>Results:</b> The research results indicate that there are 75 individuals of Collembola belonging to the class Entognatha. The dominant order observed is Entomobryomorpha, consisting of three families: Oncopoduridae with 60 individuals, Isotomidae with 7 individuals and Orchesellidae with 5 individuals. Additionally, there is the order Symphypleona, represented by a single family, Bourletiellidae, with one individual. The biodiversity index (H') yielded a moderate value of 0.622, where the most significant contribution comes from the genus Isotomidae. Meanwhile, the evenness index (E) indicates a uniform distribution among the various genera of Collembola. <b>Conclusion:</b> Observed variations in temperature, humidity and soil pH changes underscore the need for ongoing management and conservation of Baruga Forest to maintain the diversity of Collembola species and the sustainability of the ecosystem in Baruga Forest.


Assuntos
Artrópodes , Ecossistema , Humanos , Animais , Indonésia , Parques Recreativos , Florestas , Solo
14.
Virus Res ; 343: 199349, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38431055

RESUMO

BED BUGS: (Hemiptera: Cimicidae) are a globally distributed hematophagous pest that routinely feed on humans. Unlike many blood-sucking arthropods, they have never been linked to pathogen transmission in a natural setting, and despite increasing interest in their role as disease vectors, little is known about the viruses that bed bugs naturally harbor. Here, we present a global-scale survey of the bed bug RNA virosphere. We sequenced the metatranscriptomes of 22 individual bed bugs (Cimex lectularius and Cimex hemipterus) from 8 locations around the world. We detected sequences from two known bed bug viruses (Shuangao bedbug virus 1 and Shuangao bedbug virus 2) which extends their geographical range. We identified three novel bed bug virus sequences from a tenui-like virus (Bunyavirales), a toti-like virus (Ghabrivirales), and a luteo-like virus (Tolivirales). Interestingly, some of the bed bug viruses branch near to insect-transmitted plant-infecting viruses, opening questions regarding the evolution of plant virus infection. When we analyzed the viral sequences by their host's collection location, we found unexpected patterns of geographical diversity that may reflect humans' role in bed bug dispersal. Additionally, we investigated the effect that Wolbachia, the primary bed bug endosymbiont, may have on viral abundance and found that Wolbachia infection neither promotes nor inhibits viral infection. Finally, our results provide no evidence that bed bugs transmit any known human pathogenic viruses.


Assuntos
Artrópodes , Percevejos-de-Cama , Vírus , Animais , Humanos , Comportamento Alimentar , Vetores de Doenças
15.
Comp Immunol Microbiol Infect Dis ; 107: 102156, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38457963

RESUMO

Virulent species or strains of hematophagous borne pathogens such as Anaplasma spp., Babesia spp., Theileria spp., and Trypanosoma spp., are lethal to susceptible animals or reduce their productivity on a global scale. Nonetheless, efforts to diagnose the causative agents and assess the genotypic profiles as well as quantify the parasite burden of aforementioned parasites across seasons remain limited. Therefore, the present investigation sought to elucidate the genotypic composition of Anaplasma spp., Babesia spp., Theileria spp., and Trypanosoma spp. The findings revealed heightened infection rates during the summer, manifesting a correlation between Trypanosoma spp. infection and seasonal fluctuations. Among the identified pathogens, Anaplasma marginale emerged as the most dominant species, while the occurrence of Anaplasma platys in Thai cattle was confirmed via the sequencing of the groEL gene. Moreover, the study successfully identified two lineages of Trypanosoma theileri. The findings of this investigation offer valuable insights that can inform the development of preventive strategies for vector-borne diseases, such as considering the appropriate use of insect repellent, mosquito or insect nets, or eliminating breeding places for insects in each season.


Assuntos
Anaplasmose , Artrópodes , Babesia , Doenças dos Bovinos , Parasitos , Theileria , Doenças Transmitidas por Carrapatos , Trypanosoma , Animais , Bovinos , Estações do Ano , Tailândia/epidemiologia , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/parasitologia , Anaplasma/genética , Babesia/genética , Theileria/genética , Trypanosoma/genética , Anaplasmose/epidemiologia , Doenças Transmitidas por Carrapatos/veterinária
16.
J Exp Biol ; 227(Suppl_1)2024 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-38449328

RESUMO

Whether specific immune protection after initial pathogen exposure (immune memory) occurs in invertebrates has long been uncertain. The absence of antibodies, B-cells and T-cells, and the short lifespans of invertebrates led to the hypothesis that immune memory does not occur in these organisms. However, research in the past two decades has supported the existence of immune memory in several invertebrate groups, including Ctenophora, Cnidaria, Nematoda, Mollusca and Arthropoda. Interestingly, some studies have demonstrated immune memory that is specific to the parasite strain. Nonetheless, other work does not provide support for immune memory in invertebrates or offers only partial support. Moreover, the expected biphasic immune response, a characteristic of adaptive immune memory in vertebrates, varies within and between invertebrate species. This variation may be attributed to the influence of biotic or abiotic factors, particularly parasites, on the outcome of immune memory. Despite its critical importance for survival, the role of phenotypic plasticity in immune memory has not been systematically examined in the past two decades. Additionally, the features of immune responses occurring in diverse environments have yet to be fully characterized.


Assuntos
Artrópodes , Memória Imunológica , Animais , Invertebrados , Adaptação Fisiológica , Anticorpos
17.
Turkiye Parazitol Derg ; 48(1): 66-71, 2024 03 05.
Artigo em Inglês | MEDLINE | ID: mdl-38449371

RESUMO

Acanthocephaliasis is a zoonotic parasitic infection of vertebrates. The phylum Acanthocephala contains nearly 1500 acanthocephalan species. The Archiacanthocephala class is observed in terrestrial habitats and usually has a large, spineless trunk. Acanthocephalans are parasitic worms that use insects as intermediate hosts in their two-host life cycles. Insects, millipedes, and crustaceans in terrestrial areas serve as intermediate hosts and birds and mammals as definitive hosts. Acanthocephalans collected from the red fox (Vulpes vulpes) found dead on the road to Sarikamis-Kars in 1995 and stored in formaldehyde were kept in Ondokuz Mays University Faculty of Veterinary Medicine, Veterinary Parasitology Laboratory Museum until 2023 after our parasitological study found an infected red fox with Pachysentis sp. This study provides the anatomy of the acanthocephalans and the laboratory practice necessary for a good and reliable diagnosis. This study reports a new species, Pachysentis sp., of acanthocephalan (thorny-headed worm) found in red foxes for Türkiye. On the basis of relevant articles, we have created a key to Acanthocephala species occurring in mammals.


Assuntos
Acantocéfalos , Artrópodes , Humanos , Animais , Raposas , Turquia , Zoonoses
19.
Braz J Biol ; 83: e276127, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38422265

RESUMO

The study aimed to evaluate the insecticidal activity of extracts of edible mushrooms Pleurotus ostreatus and Lentinula edodes against Rhyssomatus nigerrimus. Methanol:water extracts (70:30) of P. ostreatus and L. edodes were made and evaluated in two in vitro tests (exposure and immersion toxic effect) against R. nigerrimus. Subsequently, the toxicity test of the extracts against Artemia salina was evaluated. These extracts were subjected to colorimetric tests and gas chromatography-mass spectrometry. The results showed a mortality effect against R. nigerrimus of 50% for the P. ostreatus 2 extracts at a concentration of 20% in the immersion test. Likewise, in the toxic effect test, 90% mortality was observed after five days of exposure to a concentration of 10%. On the other hand, for the toxicity test, the extract that showed the values with the highest mortality against A. salina was P. ostreatus, starting with 80% mortality at 100µg/mL. The functional groups present in the extracts were saponins, coumarins, and alkaloids. Furthermore, the presence of more than 7 compounds in the mushroom extracts evaluated is reported. This study demonstrates the insecticidal activity of P. ostreatus and L. edodes fungal extracts and indicates the importance of using different in vitro tests to elucidate the mechanism of action for future studies.


Assuntos
Artrópodes , Inseticidas , Pleurotus , Cogumelos Shiitake , Gorgulhos , Animais , Metanol , Artemia
20.
PLoS One ; 19(2): e0295377, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38335167

RESUMO

Land modification causes biodiversity loss and ecosystem modification. Despite many studies on the impacts of this factor, there is little empirical evidence on how it affects the interaction networks of plants, herbivores and their natural enemies; likewise, there is little evidence on how those networks change due to differences in the complexity of the communities they comprise. We analyzed the effects of land use and number of trophic levels on the interaction networks of exotic legume species and their associated arthropods. We collected seedpods from five exotic legume species (one of them invasive) in four land use types (urbanization, roadside, L. leucocephala plantation, wooded pasture) on Santa Cruz Island in the Galapagos, and obtained all arthropods that emerged from the seeds. Then, we built and analyzed the interaction networks for each land use at two community scales, each with different numbers of trophic levels: (1) three levels: plant-seed beetle-parasitoid (PSP), and (2) more than three levels: plant-seed beetle-parasitoid-predator and other trophic guilds (PSPP). Land use was more relevant than number of trophic levels in the configuration of species interactions. The number of species and interactions was highest on roadsides at PSPP and lowest in plantations at PSP. We found a significant effect of land use on connectance and interaction evenness (IE), and no significant effect of number of trophic levels on connectance, diversity or IE. The simultaneous analysis of land use and number of trophic levels enabled the identification of more complex patterns of community structure. Comparison of the patterns we found among islands and between exotic and native legumes is recommended. Understanding the structure of the communities analyzed here, as well as the relative contribution of their determinants of change, would allow us to develop conservation plans according to the dynamics of these neo-ecosystems.


Assuntos
Artrópodes , Ecossistema , Animais , Biodiversidade , Plantas , Herbivoria
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...