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1.
Artigo em Inglês | MEDLINE | ID: mdl-38415662

RESUMO

The misclassification of the species Pasteurella caecimuris Lagkouvardos et al. 2016 along with the heterotypic synonymy between P. caecimuris and Rodentibacter heylii Adhikary et al. 2017 has long been recognized. However, no formal assignment of P. caecimuris to its correct taxonomic position has been made accordingly and therefore the nomenclatural consequences have not been implemented. In the present study, the author first re-evaluates the taxonomic relationships of P. caecimuris using genome-based approaches, confirming the need of reclassification to the genus Rodentibacter and presenting evidence of the synonymy between R. heylii and P. caecimuris. Next, the author proposes a new name Rodentibacter caecimuris comb. nov. and, based on the priority of their specific epithets, treats Rodentibacter heylii as a later heterotypic synonym of Rodentibacter caecimuris.


Assuntos
Ácidos Graxos , Pasteurella , Pasteurellaceae , DNA Bacteriano/genética , Filogenia , RNA Ribossômico 16S/genética , Técnicas de Tipagem Bacteriana , Análise de Sequência de DNA , Composição de Bases , Ácidos Graxos/química
2.
R I Med J (2013) ; 106(11): 42-43, 2023 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-38015784

RESUMO

The complications of wound infections caused by animal related trauma are well known and explored. Of the numerous polymicrobial etiologies, Neisseria animaloris and Pasteurella canis oralis have been reported only in a limited number of cases. This manuscript explores the rare finding of these species in the case of an 83-year-old male with a diabetic foot wound complicated by infection from the saliva of his pet dog. The case highlights the first instance of P. canis oralis without the setting of a penetrating animal bite, emphasizing the vulnerability of open lesions in patients whose comorbidities impair proper wound healing. These bacteria are susceptible to beta-lactams with beta-lactamase inhibitors and can be treated once identified. It is crucial to recognize rare pathogens and initiate appropriate treatment early, and to emphasize proper wound care, especially in the context of pet interactions.


Assuntos
Osteomielite , Saliva , Masculino , Animais , Humanos , Cães , Idoso de 80 Anos ou mais , Pasteurella , Osteomielite/diagnóstico , Osteomielite/tratamento farmacológico , Osteomielite/microbiologia
3.
Nat Commun ; 14(1): 6398, 2023 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-37880229

RESUMO

The sudden mortality of African elephants (Loxodonta africana) in Botswana and Zimbabwe in 2020 provoked considerable public interest and speculation. Poaching and malicious poisoning were excluded early on in the investigation. Other potential causes included environmental intoxication, infectious diseases, and increased habitat stress due to ongoing drought. Here we show evidence of the mortalities in Zimbabwe as fatal septicaemia associated with Bisgaard taxon 45, an unnamed close relative of Pasteurella multocida. We analyse elephant carcasses and environmental samples, and fail to find evidence of cyanobacterial or other intoxication. Post-mortem and histological findings suggest a bacterial septicaemia similar to haemorrhagic septicaemia caused by P. multocida. Biochemical tests and 16S rDNA analysis of six samples and genomic analysis of one sample confirm the presence of Bisgaard taxon 45. The genome sequence contains many of the canonical P. multocida virulence factors associated with a range of human and animal diseases, including the pmHAS gene for hyaluronidase associated with bovine haemorrhagic septicaemia. Our results demonstrate that Bisgaard taxon 45 is associated with a generalised, lethal infection and that African elephants are susceptible to opportunistically pathogenic Pasteurella species. This represents an important conservation concern for elephants in the largest remaining metapopulation of this endangered species.


Assuntos
Elefantes , Septicemia Hemorrágica , Pasteurella multocida , Humanos , Animais , Bovinos , Septicemia Hemorrágica/veterinária , Septicemia Hemorrágica/microbiologia , Pasteurella , Pasteurella multocida/genética , Ecossistema
5.
BMC Vet Res ; 19(1): 128, 2023 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-37598169

RESUMO

BACKGROUND: Bacterial surface proteins play key roles in pathogenicity and often contribute to microbial adhesion and invasion. Pasteurella lipoprotein E (PlpE), a Pasteurella multocida (P. multocida) surface protein, has recently been identified as a potential vaccine candidate. Live attenuated Salmonella strains have a number of potential advantages as vaccine vectors, including immunization with live vector can mimic natural infections by organisms, lead to the induction of mucosal, humoral, and cellular immune responses. In this study, a previously constructed recombinant attenuated Salmonella Choleraesuis (S. Choleraesuis) vector rSC0016 was used to synthesize and secrete the surface protein PlpE of P. multocida to form the vaccine candidate rSC0016(pS-PlpE). Subsequently, the immunogenicity of S. Choleraesuis rSC0016(pS-PlpE) as an oral vaccine to induce protective immunity against P. multocida in mice was evaluated. RESULTS: After immunization, the recombinant attenuated S. Choleraesuis vector can efficiently delivered P. multocida PlpE protein in vivo and induced a specific immune response against this heterologous antigen in mice. In addition, compared with the inactivated vaccine, empty vector (rSC0016(pYA3493)) and PBS immunized groups, the rSC0016(pS-PlpE) vaccine candidate group induced higher antigen-specific mucosal, humoral and mixed Th1/Th2 cellular immune responses. After intraperitoneal challenge, the rSC0016(pS-PlpE) immunized group had a markedly enhanced survival rate (80%), a better protection efficiency than 60% of the inactivated vaccine group, and significantly reduced tissue damage. CONCLUSIONS: In conclusion, our study found that the rSC0016(pS-PlpE) vaccine candidate provided good protection against challenge with wild-type P. multocida serotype A in a mouse infection model, and may potentially be considered for use as a universal vaccine against multiple serotypes of P. multocida in livestock, including pigs.


Assuntos
Pasteurella multocida , Salmonella enterica , Doenças dos Suínos , Animais , Camundongos , Suínos , Pasteurella , Sorogrupo , Proteínas de Bactérias/genética , Modelos Animais de Doenças , Lipoproteínas , Proteínas de Membrana , Fatores de Transcrição , Vacinas de Produtos Inativados
6.
Sci Rep ; 13(1): 9008, 2023 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-37268660

RESUMO

Respiratory diseases caused by Mannheimia haemolytica (M. haemolytica) and Pasteurella multocida (P. multocida) have been known to result in a considerable loss due to mortality and reduced production. This study aimed at isolation and identification of M. haemolytica and P. multocida associated with pneumonic pasteurellosis in sheep and goats using bacteriological and molecular techniques. Identification of serotypes of M. haemolytica and P. multocida was done using indirect haemagglutination test. The in vitro antimicrobial sensitivity profiles of the M. haemolytica were tested using standard disk diffusion method. A total of 52 and 78 nasal swabs were collected from pneumonic cases for bacterial isolation and identification in Borana and Arsi zone, respectively. Four hundred sera samples were collected for identification of serotypes. The results showed that 17 of 52 (32.69%; 95% CI 20.33, 47.11) nasal swabs collected from pneumonic animals in Borana yielded positive results for Pasteurella/Mannheimia species, 13 (25.00%; 95% CI 14.03, 38.95) of which were M. haemolytica. None of the samples yielded P. multocida. Twenty-three of 78 (29.49%; 95% CI 19.69, 40.89) nasal swabs collected at Arsi from pneumonic animals yielded positive results for M. haemolytica (17) and P. multocida (6). Secondary biochemical characterization revealed that 14 of the 17 isolates conform to M. haemolytica whereas none of the 6 isolates suspected to be P. mutocida were confirmed. Eleven (84.62%) isolates from Borana and 4 (28.57%) from Arsi were confirmed to be M. haemolytica using PCR targeting the Rpt2 genes. Assay for M. haemolytica serotype A1 revealed all belong to A1. None of the isolates with cultural and morphological features of P. multocida gave positive results by molecular assay. Serological assay identified three serotypes of M. haemolytica namely A1, A2 and A7 almost in all of the samples whereas P. multocida serotype A was detected in 78.75% of the samples. The M. haemolytica isolates tested for susceptibility to antibiotics showed resistance against Bacitracin (83.33%) and Penicillin (50.00%) while they were found susceptible to Gentamycin (100%), Chloramphenicol (100%) and Sulfamethoxazole (100%) and Tetracycline (83.33%). In conclusion, the results of the present study revealed the association of M. haemolytica with pneumonic pasteurellosis in sheep and goats and can be of use in vaccine development in Ethiopia. Nevertheless, further investigations and continuous monitoring of antimicrobial resistance and appropriate selection and prudent use of antimicrobials in livestock sector are required.


Assuntos
Mannheimia haemolytica , Pasteurella multocida , Pasteurelose Pneumônica , Doenças dos Ovinos , Bovinos , Animais , Ovinos , Pasteurella multocida/genética , Pasteurelose Pneumônica/tratamento farmacológico , Pasteurelose Pneumônica/microbiologia , Sorogrupo , Etiópia/epidemiologia , Cabras , Pasteurella , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/microbiologia
7.
Respir Physiol Neurobiol ; 315: 104091, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37331420

RESUMO

BACKGROUND: Pasteurella (P.) multocida commonly occurs in the upper respiratory tract of healthy domestic pets, especially cats and dogs. People become infected by biting, scratching or direct contact with the animal's saliva. Inflammation develops in the wound and limits itself to the skin and subcutaneous tissue. P. multocida may cause respiratory tract infections and severe life-threatening complications. The study aimed to identify the lower respiratory infection in humans caused by P. multocida, to determine the potential source of infection and the associated symptoms, comorbidities and applied treatment. MATERIALS AND METHODS: Between January 2010 and September 2021, 14,258 patients underwent 16,255 routine flexible video bronchoscopy (FVB), and the same number of bronchoalveolar lavage fluid (BALF) samples for microbiological examination were taken. RESULTS: Microbiological examinations of the BALF only allowed the identification of six patients with P. multocida infection. All persons reported multiple scratches or bites and licking or kissing by their pets in the past. Productive cough with expectoration of mucopurulent discharge was the predominant symptom. CONCLUSIONS: A lower respiratory infection caused by P. multocida is not common in humans. It should be considered particularly in elderly patients with underlying diseases and exposure to cats and dogs.


Assuntos
Infecções por Pasteurella , Pasteurella multocida , Infecções Respiratórias , Humanos , Animais , Gatos , Cães , Idoso , Infecções por Pasteurella/diagnóstico , Infecções por Pasteurella/terapia , Infecções por Pasteurella/veterinária , Pasteurella , Saliva
8.
J AAPOS ; 27(3): 172-174, 2023 06.
Artigo em Inglês | MEDLINE | ID: mdl-37196917

RESUMO

We report an atypical presentation of endophthalmitis in a 2-month-old infant due to a rare infection by Pasteurella canis, small Gram-negative coccobacilli that inhabit the oral cavity and the gastrointestinal tracts of animals, including domesticated cats and dogs. Ocular infections are mainly associated with animal bites and scratches.


Assuntos
Endoftalmite , Infecções por Pasteurella , Pasteurella , Humanos , Endoftalmite/diagnóstico , Endoftalmite/tratamento farmacológico , Pasteurella/isolamento & purificação , Infecções por Pasteurella/diagnóstico , Infecções por Pasteurella/tratamento farmacológico , Infecções por Pasteurella/complicações , Masculino , Lactente , Hemorragia Vítrea/etiologia
9.
PLoS One ; 18(1): e0281164, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36719905

RESUMO

BACKGROUND: Acquired zoonotic infections with Pasteurella bacterial species have a wide clinical spectrum of disease from invasive infections to localised bite-wound infections. METHODS: This study reviewed the spectrum of the demographic, clinical, temporal, and microbiological trends of laboratory confirmed Pasteurella species infections presenting to a single-centre tropical tertiary hospital over a twenty-year period. RESULTS: 195 episodes from 190 patients were included. 51.3% patients were female, and 20.5% Aboriginal or Torres Strait Islander peoples. Crude incidence of Pasteurella spp. infections increased from 1.5 per 100,000 population in 2000, to 11.4 per 100,000 population in 2021. There were 22 (11.3%) bloodstream infections, 22 (11.3%) invasive, 34 (17.4%) deep local, 98 (50.2%) superficial infections, and 19 (9.7%) other or unknown. Adults over 65 years of age accounted for the majority of bacteraemias (63.7%). More severe infections, including bacteraemia, invasive and deep local infections, were more common in lower limb infections and in those with underlying comorbidities. Animal contact with cats was more common in bloodstream infections (36.4%), but dog bites more common in invasive, deep local and superficial infections. 30-day all-cause mortality was low at 1.0%. Pasteurella multocida was most commonly identified (61.1%), but P. canis, P. dagmatis, and other Pasteurella infections were also noted. 67.7% of specimens were polymicrobial, with other significant organisms being Staphylococcus aureus, Streptococcus pyogenes, Group G Streptococcus and Pseudomonas aeruginosa. CONCLUSION: Pasteurella species remain clinically important pathogens, with the ability to cause severe and invasive infections with associated morbidity. Presentations to hospital are becoming more common, and the polymicrobial nature of bites wounds has implications for empiric antibiotic guidelines.


Assuntos
Bacteriemia , Mordeduras e Picadas , Canidae , Infecções por Pasteurella , Sepse , Animais , Gatos , Cães , Feminino , Humanos , Masculino , Mordeduras e Picadas/epidemiologia , Pasteurella , Infecções por Pasteurella/veterinária , Streptococcus pyogenes , Centros de Atenção Terciária , Idoso
11.
Poult Sci ; 101(12): 102207, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36274437

RESUMO

Identification and analysis of the antimicrobial resistance of Pasteurella aerogenes (P. aerogenes) isolated from poultry. For susceptibility testing in accordance with the CLSI, plasmids were extracted via alkaline lysis and transferred by CaCl2 treatment. Genomic DNA of a representative P. aerogenes isolate was subjected to whole genome sequencing. CCCP was utilized to determine whether SF190908 contains an efflux pump. The blaVEB gene was ligated with the pET-28 plasmid and transferred to Escherichia coli to verify it as an ESBL gene. SF190908 isolated from poultry was identified as P. aerogenes based upon biochemical and 16s rRNA results. The isolate showed high MIC values for eight antimicrobials. Sequencing results showed that the mobile element-mediated antimicrobial resistance gene cluster conferred antimicrobial resistance on the strain, and a single 5,105-bp plasmid, designated pRCAD0752PA-1, was isolated. Four antimicrobial resistance gene clusters were identified in the SF190908 chromosome; one antimicrobial resistance gene cluster carried the blaVEB gene, which was verified as ESBL according to the CLSI and was detected in Pasteurellaceae for the first time, to the best of our knowledge. The efflux pump may confer antimicrobial resistance to SF190908. P. aerogenes isolated from poultry showed resistance genes encoded in mobile elements that confer multi-antimicrobial resistance to SF190908. The antimicrobial-resistant plasmid pRCAD0752PA-1 was isolated in SF190908 and conferred resistance to florfenicol. This study indicates an urgent need to increase efforts to monitor the spread of P. aerogenes multi-antimicrobial-resistant strains and plasmids, especially in newly discovered at-risk species such as poultry.


Assuntos
Infecções por Escherichia coli , Pasteurella , Animais , Pasteurella/genética , Farmacorresistência Bacteriana/genética , RNA Ribossômico 16S , Galinhas/genética , Plasmídeos/genética , Infecções por Escherichia coli/veterinária , Escherichia coli , Antibacterianos/farmacologia , beta-Lactamases/genética , Testes de Sensibilidade Microbiana/veterinária
15.
Am J Case Rep ; 23: e935270, 2022 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-35177579

RESUMO

BACKGROUND As a common member of the oral bacterial flora of cats and dogs, Pasteurella multocida can cause skin and soft tissue infection in humans after bites, licks, or scratches from animals. Uncommonly, infection due to Pasteurella can cause sepsis in humans. Even more rare is the development of infectious endocarditis from a Pasteurella infection. CASE REPORT A 76-year-old woman presented with malaise and symptoms of fluid overload. Blood cultures were positive for Pasteurella multocida, and an echocardiogram was significant for mitral valve vegetation and severe biatrial enlargement. A diagnosis of Pasteurella endocarditis was made. Surgical intervention was recommended, but owing to the risk involved, the patient elected for conservative management involving long-term treatment with intravenous antibiotics. CONCLUSIONS While exceedingly rare, Pasteurella multocida can cause infectious endocarditis in patients with predisposing factors. This patient had a known history of rheumatic heart disease, which is believed to have caused the significant findings on imaging. To the best of our knowledge, our case is the only one to depict Pasteurella endocarditis in a patient with rheumatic heart disease and severe biatrial enlargement. It is the authors' belief that the rheumatic heart disease and remodeling of the heart increased her susceptibility to severe infection from Pasteurella. The purpose of this case is to describe the pathogenicity of an otherwise low-attack bacterial infection in an elderly patient with underlying structural acquired heart damage.


Assuntos
Endocardite Bacteriana , Endocardite , Infecções por Pasteurella , Cardiopatia Reumática , Idoso , Animais , Antibacterianos/uso terapêutico , Gatos , Cães , Endocardite Bacteriana/complicações , Endocardite Bacteriana/diagnóstico , Endocardite Bacteriana/microbiologia , Feminino , Humanos , Valva Mitral/diagnóstico por imagem , Pasteurella , Infecções por Pasteurella/diagnóstico , Infecções por Pasteurella/terapia , Cardiopatia Reumática/complicações , Cardiopatia Reumática/tratamento farmacológico
16.
Eur J Clin Microbiol Infect Dis ; 41(4): 609-614, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35102471

RESUMO

Pasteurella species are infrequent but potentially severe causes of bloodstream infection (BSI). The objective of this study was to determine the incidence, risk factors, and outcomes of Pasteurella species BSI in a large Australian population. Retrospective, laboratory-based surveillance was conducted in Queensland, Australia (population ≈ 5 million) during 2000-2019, and clinical and outcome information was established by linkage to state hospital admissions and vital statistics databases. During more than 86 million person-years of surveillance, 272 incident Pasteurella species BSI occurred for an overall age- and sex-standardized annual incidence of 3.3 per million residents. The incidence of Pasteurella species BSI was highest in recent years and older individuals were at greatest risk. The median (interquartile range) Charlson Comorbidity Index was 2 (0-4) with scores of zero, 1, 2, and 3 + observed in 81 (30%), 37 (14%), 44 (16%), and 110 (40%) of cases. The 30-day all-cause case fatality was 9% (24/272) and patients who died had more comorbidities and were less likely to have community-associated disease. Although Pasteurella species are infrequent causes of BSI, older individuals and those with comorbidities are at highest risk. The burden of this disease may be expected to increase with an aging and more comorbid population.


Assuntos
Bacteriemia , Infecção Hospitalar , Sepse , Austrália , Bacteriemia/epidemiologia , Bacteriemia/microbiologia , Humanos , Incidência , Pasteurella , Queensland/epidemiologia , Estudos Retrospectivos
17.
BMC Infect Dis ; 22(1): 170, 2022 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-35189814

RESUMO

BACKGROUND: Pasteurella multocida is a well-known gram-negative facultative anaerobe well known for its ability to cause soft tissue infections following animal bite or scratch. Here we present a case with mycotic aneurysm of the superficial femoral artery due to P. multocida infection. CASE PRESENTATION: A 62 year old male patient presented with worsening right leg pain and swelling. On examination, he was found to have profound swelling and erythema of the right medial thigh and tenderness to palpation. Computerized tomography showed findings suggestive of right femoral pseudoaneurysm with a large right medial thigh hematoma. Blood cultures grew P. multocida. Patient underwent emergent open resection of the mycotic aneurysm and vascular bypass surgery. Intraoperatively, the site was noted to be grossly infected with multiple pockets of pus which were drained and pus cultures grew P. multocida. The diagnosis of P. multocida bacteremia with right femoral mycotic aneurysm and thigh abscess was made. Patient received 6 weeks of intravenous ceftriaxone and recovered. CONCLUSION: Our case is the first report on infection of peripheral vessel with Pasteurella and highlights the importance of prompt surgical intervention and effective antibiotic treatment.


Assuntos
Falso Aneurisma , Aneurisma Infectado , Infecções por Pasteurella , Falso Aneurisma/diagnóstico por imagem , Falso Aneurisma/cirurgia , Aneurisma Infectado/diagnóstico por imagem , Aneurisma Infectado/cirurgia , Animais , Artéria Femoral/diagnóstico por imagem , Artéria Femoral/cirurgia , Hematoma/diagnóstico por imagem , Hematoma/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Pasteurella , Infecções por Pasteurella/diagnóstico , Infecções por Pasteurella/etiologia
19.
Int J Infect Dis ; 116: 197-203, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35063680

RESUMO

BACKGROUND: Pasteurella spp. can lead to fatal infections in humans. OBJECTIVE: To assess prognostic factors of invasive pasteurellosis. METHODS: We conducted a single retrospective cohort study of local versus invasive Pasteurella infections from January 1, 2005, to December 31, 2018, in the Amiens-Picardie University Hospital, France. RESULTS: Forty-five (20.9%) invasive pasteurellosis and 22 (10.2%) complicated local infections were reported among a total of 215 Pasteurella infections. The mortality rate among invasive infections was 22.2% (10/ 45) whereas no death was recorded in local infections group. Non-drug-induced prothrombin time test <70% of standard and platelet counts <100,000/mm3 were more frequent in non-survivors than in survivors (p=0.005 and p=0.019) in univariate analyses. A history of neoplasia (adjusted OR=13.62, p=0.020), an evidence of bacteremia (adjusted OR=20.68, p=0.025), and hemoglobin level <10 g/dL (adjusted OR=17.80, p=0.028) were identified as poor prognostic factors in multivariate analyses. CONCLUSION: Invasive pasteurellosis appears as a serious disease in vulnerable patients, particularly if bacteremia and/or coagulopathies occur.


Assuntos
Bacteriemia , Infecções por Pasteurella , Bacteriemia/complicações , Bacteriemia/diagnóstico , Bacteriemia/epidemiologia , Humanos , Pasteurella , Infecções por Pasteurella/complicações , Infecções por Pasteurella/diagnóstico , Infecções por Pasteurella/epidemiologia , Prognóstico , Estudos Retrospectivos
20.
J Fish Dis ; 45(1): 153-163, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34719037

RESUMO

Lumpfish is a novel farmed species used as cleaner fish for the removal of lice from farmed salmon. As often with new, farmed species, there are challenges with bacterial infections. The frequency of prescription of antibiotic agents to lumpfish is increasing, despite the lack of knowledge about appropriate doses, duration of treatment and application protocols for the various antibacterial agents. In the current study, we have tested the effect of medicated feed with florfenicol (FFC), oxolinic acid (OA) and flumequine (FLU) on lumpfish experimentally challenged with Vibrio anguillarum, atypical Aeromonas salmonicida and Pasteurella atlantica. We found that all three antibacterial agents efficiently treated lumpfish with vibriosis using 10 and 20 mg kg-1  day-1 of FFC, 25 mg kg-1  day-1 of OA and 25 mg kg-1  day-1 FLU, whereas only FFC (20 mg kg-1  day-1 ) had good effect on lumpfish with pasteurellosis. None of the antibacterial agents were efficient to treat lumpfish with atypical furunculosis. FFC 20 mg kg-1  day-1 showed promising results in the beginning of the experiment, but the mortality increased rapidly 14 days post-medication. Efficient treatment is important for the welfare of lumpfish and for reducing the risk of development of antibiotic-resistant bacteria. To our knowledge, this is the first study to establish protocols for antibacterial treatment of lumpfish.


Assuntos
Aeromonas salmonicida , Doenças dos Peixes , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Doenças dos Peixes/tratamento farmacológico , Pasteurella , Vibrio
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