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1.
Sci Rep ; 14(1): 5986, 2024 03 12.
Artigo em Inglês | MEDLINE | ID: mdl-38472251

RESUMO

Lead (Pb) is toxic to the development and growth of rice plants. Nanoparticles (NPs) have been considered one of the efficient remediation techniques to mitigate Pb stress in plants. Therefore, a study was carried out to examine the underlying mechanism of iron (Fe) and silicon (Si) nanoparticle-induced Pb toxicity alleviation in rice seedlings. Si-NPs (2.5 mM) and Fe-NPs (25 mg L-1) were applied alone and in combination to rice plants grown without (control; no Pb stress) and with (100 µM) Pb concentration. Our results revealed that Pb toxicity severely affected all rice growth-related traits, such as inhibited root fresh weight (42%), shoot length (24%), and chlorophyll b contents (26%). Moreover, a substantial amount of Pb was translocated to the above-ground parts of plants, which caused a disturbance in the antioxidative enzyme activities. However, the synergetic use of Fe- and Si-NPs reduced the Pb contents in the upper part of plants by 27%. It reduced the lethal impact of Pb on roots and shoots growth parameters by increasing shoot length (40%), shoot fresh weight (48%), and roots fresh weight (31%). Both Si and Fe-NPs synergistic application significantly elevated superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione (GSH) concentrations by 114%, 186%, 135%, and 151%, respectively, compared to plants subjected to Pb stress alone. The toxicity of Pb resulted in several cellular abnormalities and altered the expression levels of metal transporters and antioxidant genes. We conclude that the synergistic application of Si and Fe-NPs can be deemed favorable, environmentally promising, and cost-effective for reducing Pb deadliness in rice crops and reclaiming Pb-polluted soils.


Assuntos
Nanopartículas , Oryza , Poluentes do Solo , Oryza/genética , Silício/farmacologia , Chumbo/metabolismo , Ferro/metabolismo , Antioxidantes/metabolismo , Glutationa/metabolismo , Superóxido Dismutase/metabolismo , Estresse Oxidativo , Poluentes do Solo/metabolismo
2.
Cell Mol Life Sci ; 81(1): 126, 2024 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-38470510

RESUMO

Stress-induced intestinal epithelial injury (IEI) and a delay in repair in infancy are predisposing factors for refractory gut diseases in adulthood, such as irritable bowel syndrome (IBS). Hence, it is necessary to develop appropriate mitigation methods for mammals when experiencing early-life stress (ELS). Weaning, as we all know, is a vital procedure that all mammalian newborns, including humans, must go through. Maternal separation (MS) stress in infancy (regarded as weaning stress in animal science) is a commonly used ELS paradigm. Drinking silicon-rich alkaline mineral water (AMW) has a therapeutic effect on enteric disease, but the specific mechanisms involved have not been reported. Herein, we discover the molecular mechanism by which silicon-rich AMW repairs ELS-induced IEI by maintaining intestinal stem cell (ISC) proliferation and differentiation through the glucagon-like peptide (GLP)2-Wnt1 axis. Mechanistic study showed that silicon-rich AMW activates GLP2-dependent Wnt1/ß-catenin pathway, and drives ISC proliferation and differentiation by stimulating Lgr5+ ISC cell cycle passage through the G1-S-phase checkpoint, thereby maintaining intestinal epithelial regeneration and IEI repair. Using GLP2 antagonists (GLP23-33) and small interfering RNA (SiWnt1) in vitro, we found that the GLP2-Wnt1 axis is the target of silicon-rich AMW to promote intestinal epithelium regeneration. Therefore, silicon-rich AMW maintains intestinal epithelium regeneration through the GLP2-Wnt1 axis in piglets under ELS. Our research contributes to understanding the mechanism of silicon-rich AMW promoting gut epithelial regeneration and provides a new strategy for the alleviation of ELS-induced IEI.


Assuntos
Experiências Adversas da Infância , Águas Minerais , Recém-Nascido , Humanos , Animais , Suínos , Silício/metabolismo , Privação Materna , Mucosa Intestinal/metabolismo , Mamíferos
3.
PLoS One ; 19(3): e0300024, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38470890

RESUMO

Today, with the advent of Large-scale generative Language Models (LLMs) it is now possible to simulate free responses to interview questions such as those traditionally analyzed using qualitative research methods. Qualitative methodology encompasses a broad family of techniques involving manual analysis of open-ended interviews or conversations conducted freely in natural language. Here we consider whether artificial "silicon participants" generated by LLMs may be productively studied using qualitative analysis methods in such a way as to generate insights that could generalize to real human populations. The key concept in our analysis is algorithmic fidelity, a validity concept capturing the degree to which LLM-generated outputs mirror human sub-populations' beliefs and attitudes. By definition, high algorithmic fidelity suggests that latent beliefs elicited from LLMs may generalize to real humans, whereas low algorithmic fidelity renders such research invalid. Here we used an LLM to generate interviews with "silicon participants" matching specific demographic characteristics one-for-one with a set of human participants. Using framework-based qualitative analysis, we showed the key themes obtained from both human and silicon participants were strikingly similar. However, when we analyzed the structure and tone of the interviews we found even more striking differences. We also found evidence of a hyper-accuracy distortion. We conclude that the LLM we tested (GPT-3.5) does not have sufficient algorithmic fidelity to expect in silico research on it to generalize to real human populations. However, rapid advances in artificial intelligence raise the possibility that algorithmic fidelity may improve in the future. Thus we stress the need to establish epistemic norms now around how to assess the validity of LLM-based qualitative research, especially concerning the need to ensure the representation of heterogeneous lived experiences.


Assuntos
Inteligência Artificial , Silício , Humanos , Idioma , Comunicação , Cafeína
4.
Int J Mol Sci ; 25(5)2024 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-38473773

RESUMO

This article aims to develop an aspirin-loaded double-modified nano-delivery system for the treatment of hepatocellular carcinoma. In this paper, mesoporous silica nanoparticles (MSN) were prepared by the "one-pot two-phase layering method", and polydopamine (PDA) was formed by the self-polymerization of dopamine as a pH-sensitive coating. Gal-modified PDA-modified nanoparticles (Gal-PDA-MSN) were synthesized by linking galactosamine (Gal) with actively targeted galactosamine (Gal) to PDA-coated MSN by a Michael addition reaction. The size, particle size distribution, surface morphology, BET surface area, mesoporous size, and pore volume of the prepared nanoparticles were characterized, and their drug load and drug release behavior in vitro were investigated. Gal-PDA-MSN is pH sensitive and targeted. MSN@Asp is different from the release curves of PDA-MSN@Asp and Gal-PDA-MSN@Asp, the drug release of PDA-MSN@Asp and Gal-PDA-MSN@Asp accelerates with increasing acidity. In vitro experiments showed that the toxicity and inhibitory effects of the three nanodrugs on human liver cancer HepG2 cells were higher than those of free Asp. This drug delivery system facilitates controlled release and targeted therapy.


Assuntos
Neoplasias Hepáticas , Nanopartículas , Humanos , Silício , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Nanopartículas/química , Dióxido de Silício/química , Concentração de Íons de Hidrogênio , Galactosamina
5.
Nature ; 627(8003): 313-320, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38480964

RESUMO

Intrinsically stretchable electronics with skin-like mechanical properties have been identified as a promising platform for emerging applications ranging from continuous physiological monitoring to real-time analysis of health conditions, to closed-loop delivery of autonomous medical treatment1-7. However, current technologies could only reach electrical performance at amorphous-silicon level (that is, charge-carrier mobility of about 1 cm2 V-1 s-1), low integration scale (for example, 54 transistors per circuit) and limited functionalities8-11. Here we report high-density, intrinsically stretchable transistors and integrated circuits with high driving ability, high operation speed and large-scale integration. They were enabled by a combination of innovations in materials, fabrication process design, device engineering and circuit design. Our intrinsically stretchable transistors exhibit an average field-effect mobility of more than 20 cm2 V-1 s-1 under 100% strain, a device density of 100,000 transistors per cm2, including interconnects and a high drive current of around 2 µA µm-1 at a supply voltage of 5 V. Notably, these achieved parameters are on par with state-of-the-art flexible transistors based on metal-oxide, carbon nanotube and polycrystalline silicon materials on plastic substrates12-14. Furthermore, we realize a large-scale integrated circuit with more than 1,000 transistors and a stage-switching frequency greater than 1 MHz, for the first time, to our knowledge, in intrinsically stretchable electronics. Moreover, we demonstrate a high-throughput braille recognition system that surpasses human skin sensing ability, enabled by an active-matrix tactile sensor array with a record-high density of 2,500 units per cm2, and a light-emitting diode display with a high refreshing speed of 60 Hz and excellent mechanical robustness. The above advancements in device performance have substantially enhanced the abilities of skin-like electronics.


Assuntos
Desenho de Equipamento , Pele , Transistores Eletrônicos , Dispositivos Eletrônicos Vestíveis , Humanos , Silício , Nanotubos de Carbono , Tato
6.
BMC Ophthalmol ; 24(1): 112, 2024 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-38454351

RESUMO

INTRODUCTION: Severe congenital ptosis poses a complex challenge for oculoplastic surgeons, requiring meticulous surgical intervention to restore eyelid function and improve aesthetic outcomes mainly by using frontalis sling approach. A crucial issue in frontalis sling surgeries is the sustainability of effect. PURPOSE: This retrospective study reports the outcomes of two surgical techniques for treating severe congenital ptosis in the paediatric age group: Silicon rods ptosis sling and a novel technique involving the use of Silicon rods with green braided polyester (Ethibond) sutures to secure the rods in place "sling for the sling". METHODS: The medical records of children who underwent frontalis suspension were reviewed in a retrospective fashion. We identified two groups; the first group (20 patients: 35 eyelids) had the traditional frontalis suspension surgery using silicone suspension set, the second group (14 patients: 25 eyelids) was operated using the new "sling for sling" technique. We used the postoperative marginal reflex distance-1 (MRD-1) as the primary outcome measure while the frequency of both wound related complications and recurrence were considered as secondary outcome measures. Post operative data were collected and compared after 1 month, 6 months, 12 months, and 18 months. RESULTS: Preliminary results indicate promising outcomes for both techniques, with significant improvement in eyelid elevation observed in both groups. However, the novel technique using Silicon rods with Ethibond sutures demonstrated enhanced sustainability, leading to a more durable outcome with significantly less recurrence. CONCLUSION: This study highlights the potential benefits of the novel technique in treating severe congenital ptosis and introduces an innovative approach to Silicone rods fixation to achieve a long-term corrective effect.


Assuntos
Blefaroplastia , Blefaroptose , Criança , Humanos , Blefaroplastia/métodos , Estudos Retrospectivos , Silício , Técnicas de Sutura , Blefaroptose/cirurgia , Blefaroptose/congênito , Silicones , Músculos Oculomotores/cirurgia , Resultado do Tratamento
7.
Sci Rep ; 14(1): 6380, 2024 03 16.
Artigo em Inglês | MEDLINE | ID: mdl-38493184

RESUMO

Globally, salinity is an important abiotic stress in agriculture. It induced oxidative stress and nutritional imbalance in plants, resulting in poor crop productivity. Applying silicon (Si) can improve the uptake of macronutrients. On the other hand, using biochar as a soil amendment can also decrease salinity stress due to its high porosity, cation exchange capacity, and water-holding capacity. That's why the current experiment was conducted with novelty to explore the impact of silicon nanoparticle-based biochar (Si-BC) on wheat cultivated on salt-affected soil. There were 3 levels of Si-BC, i.e., control (0), 1% Si-BC1, and 2.5% Si-BC2 applied in 3 replicates under 0 and 200 mM NaCl following a completely randomized design. Results showed that treatment 2.5% Si-BC2 performed significantly better for the enhancement in shoot and root length, shoot and root fresh weight, shoot and root dry weight, number of leaves, number of tillers, number of spikelets, spike length, spike fresh and dry weight compared to control under no stress and salinity stress (200 mM NaCl). A significant enhancement in chlorophyll a (~ 18%), chlorophyll b (~ 22%), total chlorophyll (~ 20%), carotenoid (~ 60%), relative water contents (~ 58%) also signified the effectiveness of treatment 2.5% Si-BC2 than control under 200 mM NaCl. In conclusion, treatment 2.5% Si-BC2 can potentially mitigate the salinity stress in wheat by regulating antioxidants and improving N, K concentration, and gas exchange attributes while decreasing Na and Cl concentration and electrolyte leakage. More investigations at the field level are recommended for the declaration of treatment 2.5% Si-BC2 as the best amendment for alleviating salinity stress in different crops under variable climatic conditions.


Assuntos
Antioxidantes , Carvão Vegetal , Cloreto de Sódio , Antioxidantes/farmacologia , Cloreto de Sódio/farmacologia , Silício/farmacologia , Triticum , Clorofila A , Estresse Salino , Solo , Nutrientes , Água/farmacologia , Salinidade
8.
Environ Pollut ; 346: 123691, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38431245

RESUMO

The excessive accumulation of heavy metals, particularly lead (Pb) in agricultural soils, is a growing problem worldwide and needs urgent attention. This study aimed to prepare green silicon (Si) NPs using extract of Chenopodium quinoa leaves and evaluated their effects on Pb uptake and growth of maize (Zea mays L.). The results indicated that Pb exposure negatively affected the growth and chlorophyll contents of maize varieties, while SiNPs positively affected these attributes. Pb alone increased the electrolyte-leakage (EL), hydrogen-peroxide (H2O2) and selected antioxidant enzyme activities in leaves, whereas SiNPs decreased EL and H2O2 concentrations and further enhanced the enzyme activities as compared to their respective treatments without SiNPs. Pb-only treatments led to an increase in Pb concentrations and total Pb uptake in both shoots and roots. In contrast, SiNPs resulted in reduced Pb concentrations, with a concurrent decrease in total Pb uptake in shoots compared to the control treatment. The findings demonstrated that foliar application of SiNPs can mitigate the toxic effects of Pb in maize plants by triggering the antioxidant enzyme system and reducing the oxidative stress. Taken together, SiNPs have the potential to enhance maize production in Pb-contaminated soils. However, future research and application efforts should prioritize key aspects such as optimizing NPs synthesis, understanding positive mechanisms of green-synthesized NPs, and conducting multiple crop tests and real-world field trials.


Assuntos
Nanopartículas , Poluentes do Solo , Zea mays , Antioxidantes/farmacologia , Silício/farmacologia , Chumbo/toxicidade , Peróxido de Hidrogênio/farmacologia , Nanopartículas/toxicidade , Solo , Poluentes do Solo/análise
9.
Sci Rep ; 14(1): 6176, 2024 03 14.
Artigo em Inglês | MEDLINE | ID: mdl-38486015

RESUMO

Arsenic (As) is a heavy metal that is toxic to both plants and animals. Silicon nanoparticles (SiNPs) can alleviate the detrimental effects of heavy metals on plants, but the underlying mechanisms remain unclear. The study aims to synthesize SiNPs and reveal how they promote plant health in Arsenic-polluted soil. 0 and 100% v/v SiNPs were applied to soil, and Arsenic 0 and 3.2 g/ml were applied twice. Maize growth was monitored until maturity. Small, irregular, spherical, smooth, and non-agglomerated SiNPs with a peak absorbance of 400 nm were synthesized from Pycreus polystachyos. The SiNPs (100%) assisted in the development of a deep, prolific root structure that aided hydraulic conductance and gave mechanical support to the maize plant under As stress. Thus, there was a 40-50% increase in growth, tripled yield weights, and accelerated flowering, fruiting, and senescence. SiNPs caused immobilization (As(III)=SiNPs) of As in the soil and induced root exudates Phytochelatins (PCs) (desGly-PC2 and Oxidized Glutathione) which may lead to formation of SiNPs=As(III)-PCs complexes and sequestration of As in the plant biomass. Moreover, SiNPs may alleviate Arsenic stress by serving as co-enzymes that activate the antioxidant-defensive mechanisms of the shoot and root. Thus, above 70%, most reactive ROS (OH) were scavenged, which was evident in the reduced MDA content that strengthened the plasma membrane to support selective ion absorption of SiNPs in place of Arsenic. We conclude that SiNPs can alleviate As stress through sequestration with PCs, improve root hydraulic conductance, antioxidant activity, and membrane stability in maize plants, and could be a potential tool to promote heavy metal stress resilience in the field.


Assuntos
Arsênio , Metais Pesados , Nanopartículas , Animais , Arsênio/metabolismo , Silício/farmacologia , Zea mays/metabolismo , Metais Pesados/metabolismo , Antioxidantes/metabolismo , Plantas/metabolismo , Fitoquelatinas/metabolismo , Nanopartículas/química , Solo
10.
Biol Lett ; 20(3): 20230451, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38442870

RESUMO

Elevated leaf silicon (Si) concentrations improve drought resistance in cultivated plants, suggesting Si might also improve drought performance of wild species. Tropical tree species, for instance, take up substantial amounts of Si, and leaf Si varies markedly at local and regional scales, suggesting consequences for seedling drought resistance. Yet, whether elevated leaf Si improves seedling drought performance in tropical forests is unknown. To manipulate leaf Si concentrations, seedlings of seven tropical tree species were grown in Si-rich and -poor soil, before exposing them to drought in the forest understorey. Survival, growth and wilting were monitored. Elevated leaf Si did not improve drought survival and growth in any of the species. In one species, drought survival was reduced in seedlings previously grown in Si-rich soil, contrary to our expectation. Our results suggest that elevated leaf Si does not improve drought resistance of wild tropical tree species. Elevated leaf Si may even reduce drought performance, suggesting differences in soil conditions influencing leaf Si may contribute to soil-related variation of tropical seedling performance. Furthermore, our results are at odds with most studies on cultivated species and show that alleviative effects of Si in crops cannot be generalized to wild plants in natural systems.


Assuntos
Plântula , Árvores , Secas , Silício/farmacologia , Folhas de Planta , Solo
11.
Biosens Bioelectron ; 253: 116194, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38467100

RESUMO

The multiplexed detection of metabolites in parallel within a single biosensor plate is sufficiently valuable but also challenging. Herein, we combine the inherent light addressability of silicon with the high selectivity of enzymes, for the construction of multiplexed photoelectrochemical enzymatic biosensors. To conduct a stable electrochemistry and reagentless biosensing on silicon, a new strategy involving the immobilization of both redox mediators and enzymes using an amide bond-based hydrogel membrane was proposed. The membrane characterization results demonstrated a covalent coupling of ferrocene mediator to hydrogel, in which the mediator acted as not only a signal generator but also a renewable sacrifice agent. By adding corresponding enzymes on different spots of hydrogel membrane modified silicon and recording local photocurrents with a moveable light pointer, this biosensor setup was used successfully to detect multiple metabolites, such as lactate, glucose, and sarcosine, with good analytical performances. The limits of detection of glucose, sarcosine and lactate were found to be 179 µM, 16 µM, and 780 µM with the linear ranges of 0.5-2.5 mM, 0.3-1.5 mM, and 1.0-3.0 mM, respectively. We believe this proof-of-concept study provides a simple and rapid one-step immobilization approach for the fabrication of reagentless enzymatic assays with silicon-based light-addressable electrochemistry.


Assuntos
Técnicas Biossensoriais , Silício , Eletroquímica/métodos , Sarcosina , Técnicas Biossensoriais/métodos , Hidrogéis , Lactatos , Glucose
12.
Anal Chim Acta ; 1298: 342415, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38462342

RESUMO

BACKGROUND: Tyramine is an important index of food freshness degree, and tyrosinase that can specifically oxidized monophenolamine to catecholamine plays a crucial part in the occurrence and development of melanin-related skin diseases. Therefore, it is crucial to develop sensitive and efficient methods for the detection of tyramine and tyrosinase. RESULTS: In this work, encouraged by tyrosinase-triggered specific oxidation of tyramine to dopamine and the unique fluorescent reaction between dopamine and amino silane, we have developed a one-step synthetic strategy of silicon containing nanoparticles (Si CNPs) for "turn-on" detection of tyramine and tyrosinase. The Si CNPs formed with thoroughly studied mechanism exhibit uniform structure and robust yellow-green fluorescence. The low detection limits for tyramine (1.87 µM) and tyrosinase (0.0029 U/mL) demonstrate admirable sensitivity outstripping most methods. The proposed assay achieves satisfactory results in the determination of tyramine and tyrosinase activity in real samples. Furthermore, we leverage this new fluorescent assay to enable the fabrication of an "AND" Boolean logic gate. SIGNIFICANCE: The entire process can be completed at easily available temperature and pressure with rapid response, convenient operation and visual observation. This fluorescent assay featured with excellent sensitivity, selectivity and stability has considerable prospects in the application of biosensors and disease diagnosis.


Assuntos
Monofenol Mono-Oxigenase , Nanopartículas , Monofenol Mono-Oxigenase/química , Dopamina/química , Silício , Tiramina , Nanopartículas/química
13.
Biomed Mater ; 19(3)2024 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-38387060

RESUMO

The eco-friendly and scalable production of bioglass remains a challenging but attractive strategy for advancing its widespread biomedical applications. Although the sol-gel method has been considered a valuable approach for bioglass production, the application of calcium nitrate as a calcium source markedly limits its industrialization owing to environmental pollution, high administration costs, and numerous calcium-rich regions in the as-prepared bioglass. Therefore, organic Ca has been proposed as an alternative to inorganic Ca. In the current study, bioglass was successfully prepared using a novel calcium source (calcium glycerol) and was named regeneration silicon (RegeSi). The biocompatibity of bioglass was examined by performing the methyl thiazolyl tetrazolium (MTT) assay using L929 fibroblasts. The biological and tissue repair properties of RegeSi were better than those of bioglass prepared with calcium nitrate using the sol-gel or traditional melting methods. The applicability of RegeSi was validated using suitable wound healing and dental restoration models. Notably, RegeSi ensured closure of a deep wound (1.6 cm diameter, 2 mm depth) within 11 d. Moreover, RegeSi facilitated tooth repair with a blocking rate of 97.1%. More importantly, large-scale production of RegeSi was achieved at low cost, high bioactivity, and using environmental technology, reaching a capacity of 100 kg/batch.


Assuntos
Compostos de Cálcio , Cálcio , Nitratos , Cerâmica , Silício , Cicatrização
14.
Spectrochim Acta A Mol Biomol Spectrosc ; 311: 123991, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38330763

RESUMO

The ability of fluorescence hyperspectral imaging to predict heavy metal lead (Pb) concentration in oilseed rape leaves was studied in silicon-free and silicon environments. Further, the transfer stacked convolution auto-encoder (T-SCAE) algorithm was proposed based on the stacked convolution auto-encoder (SCAE) algorithm. Fluorescence hyperspectral images of oilseed rape leaves under different Pb stress contents were obtained in the silicon-free and silicon environments. The entire region of oilseed rape leaves was chosen as the region of interest (ROI) to obtain fluorescence spectra. First of all, standard normalized variable (SNV) algorithm was implemented as the preferred preprocessing method, and the fluorescence spectral data processed by SNV was utilized for further analysis. Further, SCAE was used to reduce the dimensionality of the best pre-processed spectral data, and compared with the traditional dimensionality reduction algorithm. Finally, the optimal SCAE deep learning network was transferred to obtain the T-SCAE model to verify the transferability between the deep learning models in silicon-free and silicon environments. The results show that the SVR model based on the depth features extracted by SCAE has the best performance in predicting different Pb concentrations in silicon-free or silicon environments, and the coefficient of determination (Rp2), root mean square error (RMSEP) and residual predictive deviation (RPD) of prediction set in silicon-free or silicon environments were 0.9374, 0.02071 mg/kg and 3.268, and 0.9416, 0.01898 mg/kg and 3.316, respectively. Moreover, the SVR model based on the depth feature extracted by T-SCAE has the best performance in predicting different Pb concentrations in silicon-free and silicon environments, and the Rp2, RMSEP and RPD of the optimal prediction set were 0.9385, 0.02017 mg/kg and 3.291, respectively. The combination of hyperspectral fluorescence imaging and deep transfer learning algorithm can effectively detect different Pb concentrations in oilseed rape leaves in both non-silicon environment and silicon environment.


Assuntos
Brassica napus , Chumbo , Silício , Imageamento Hiperespectral , Análise dos Mínimos Quadrados , Algoritmos , Folhas de Planta , Aprendizado de Máquina
15.
Sci Rep ; 14(1): 4717, 2024 02 27.
Artigo em Inglês | MEDLINE | ID: mdl-38413655

RESUMO

Aluminum (Al) inhibits growth and limits plant productivity in acidic soils. An important strategy to increase Al tolerance is the use of silicon (Si) nutrition. Thus, the aim of this study was to evaluate the interactive role of Si in increasing the growth, physiological and morphoanatomy responses of sugarcane plants under Al toxicity. A 4 × 2 factorial scheme in a completely randomized design was used to study the impact of Si (2 mM) on attenuating Al toxicity (0, 10, 15 and 20 mg L-1, as Al2(SO4)3·18H2O) in sugarcane seedlings. After 45 days, Al toxicity affected sugarcane growth by increasing Al uptake and accumulation, modifying root growth, thickness, and morphoanatomy, and decreasing pigment content, gas exchange parameters, and the number of adaxial and abaxial stomata. However, Si attenuated Al toxicity in the sugarcane seedlings by limiting Al uptake and transport to the shoots, causing positive changes in root morphoanatomy, higher pigment content, improving gas exchange parameters, thereby increased growth. Furthermore, cultivar 'CTC9003' showed beneficial impacts from Si supplementation than 'CTC9002', especially under Al toxicity. The findings of this study suggest that Si plays a notable role in improving anatomical and physiological aspects, particularly the growth of sugarcane seedlings under Al toxicity.


Assuntos
Saccharum , Silício , Silício/farmacologia , Alumínio/toxicidade , Fotossíntese , Plantas , Plântula , Raízes de Plantas
16.
Plant Physiol Biochem ; 207: 108416, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38354528

RESUMO

Silicon (Si) and selenium (Se) can improve the tolerance of plants to NaCl-induced salt stress. However, few studies are available on their regulatory effects on plants' tolerance to calcium nitrate stress, which often occurs in protected facilities, causing secondary soil salinization. In this study, we report the effects of Si (6 mM) and Se (20 µM) applied separately or in combination on the growth, photosynthesis, oxidative damage, and nitrogen metabolism of tomato plants, as well as fruit quality under calcium nitrate stress. The results showed that applications of Si or Se alone or in combination improved the plant growth and photosynthetic performance and reduced oxidative damage of the stressed plants. Applications of Si and Se did not decrease the calcium accumulation in leaves of the stressed plants. Under calcium nitrate stress, the concentrations of NO3-, NO2- and NH4+ in leaves were significantly increased, while the activities of nitrogen assimilation-related enzymes (including nitrate reductase, nitrite reductase, glutamine synthase, glutamine-2-oxoglutarate aminotransferase and glutamate dehydrogenase) were decreased. Applications of Si and Se, especially their combined treatment, decreased the NO3-, NO2-, and NH4+ concentrations and enhanced the activities of nitrogen assimilation-related enzymes in the stressed plants. Applied Si and Se also decreased the nitrate and titratable acid concentrations and increased vitamin levels in tomato fruits under calcium nitrate stress. It is suggested that Si and Se improved the tomato plant growth and fruit quality under calcium nitrate stress by alleviating oxidative damage and promoting both photosynthesis and nitrogen assimilation.


Assuntos
Compostos de Cálcio , Selênio , Solanum lycopersicum , Nitratos/farmacologia , Nitratos/metabolismo , Selênio/farmacologia , Silício/farmacologia , Dióxido de Nitrogênio , Glutamina , Nitrogênio/metabolismo
17.
Mediators Inflamm ; 2024: 3188216, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38385005

RESUMO

Background: Rheumatoid arthritis (RA) remains one of the most prevalent chronic joint diseases. However, due to the heterogeneity among RA patients, there are still no robust diagnostic and therapeutic biomarkers for the diagnosis and treatment of RA. Methods: We retrieved RA-related and pan-cancer information datasets from the Gene Expression Omnibus and The Cancer Genome Atlas databases, respectively. Six gene expression profiles and corresponding clinical information of GSE12021, GSE29746, GSE55235, GSE55457, GSE77298, and GSE89408 were adopted to perform differential expression gene analysis, enrichment, and immune component difference analyses of RA. Four machine learning algorithms, including LASSO, RF, XGBoost, and SVM, were used to identify RA-related biomarkers. Unsupervised cluster analysis was also used to decipher the heterogeneity of RA. A four-signature-based nomogram was constructed and verified to specifically diagnose RA and osteoarthritis (OA) from normal tissues. Consequently, RA-HFLS cell was utilized to investigate the biological role of CRTAM in RA. In addition, comparisons of diagnostic efficacy and biological roles among CRTAM and other classic biomarkers of RA were also performed. Results: Immune and stromal components were highly enriched in RA. Chemokine- and Th cell-related signatures were significantly activated in RA tissues. Four promising and novel biomarkers, including CRTAM, PTTG1IP, ITGB2, and MMP13, were identified and verified, which could be treated as novel treatment and diagnostic targets for RA. Nomograms based on the four signatures might aid in distinguishing and diagnosing RA, which reached a satisfactory performance in both training (AUC = 0.894) and testing (AUC = 0.843) cohorts. Two distinct subtypes of RA patients were identified, which further verified that these four signatures might be involved in the immune infiltration process. Furthermore, knockdown of CRTAM could significantly suppress the proliferation and invasion ability of RA cell line and thus could be treated as a novel therapeutic target. CRTAM owned a great diagnostic performance for RA than previous biomarkers including MMP3, S100A8, S100A9, IL6, COMP, LAG3, and ENTPD1. Mechanically, CRTAM could also be involved in the progression through immune dysfunction, fatty acid metabolism, and genomic instability across several cancer subtypes. Conclusion: CRTAM, PTTG1IP, ITGB2, and MMP13 were highly expressed in RA tissues and might function as pivotal diagnostic and treatment targets by deteriorating the immune dysfunction state. In addition, CRTAM might fuel cancer progression through immune signals, especially among RA patients.


Assuntos
Artrite Reumatoide , Neoplasias , Humanos , Silício , Metaloproteinase 13 da Matriz , Biomarcadores , Algoritmos
18.
Sensors (Basel) ; 24(4)2024 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-38400464

RESUMO

This article introduces an algorithm for detecting glucose and silicon levels in solution. The research focuses on addressing the critical need for accurate and efficient glucose monitoring, particularly in the context of diabetic management. Understanding and monitoring silicon levels in the body is crucial due to its significant role in various physiological processes. Silicon, while often overshadowed by other minerals, plays a vital role in bone health, collagen formation, and connective tissue integrity. Moreover, recent research suggests its potential involvement in neurological health and the prevention of certain degenerative diseases. Investigating silicon levels becomes essential for a comprehensive understanding of its impact on overall health and well-being and paves the way for targeted interventions and personalized healthcare strategies. The approach presented in this paper is based on the integration of hyperspectral data and artificial intelligence techniques. The algorithm investigates the effectiveness of two distinct models utilizing SVMR and a perceptron independently. SVMR is employed to establish a robust regression model that maps input features to continuous glucose and silicon values. The study outlines the methodology, including feature selection, model training, and evaluation metrics. Experimental results demonstrate the algorithm's effectiveness at accurately predicting glucose and silicon concentrations and showcases its potential for real-world application in continuous glucose and silicon monitoring systems.


Assuntos
Inteligência Artificial , Glucose , Silício , Automonitorização da Glicemia , Imageamento Hiperespectral , Glicemia , Aprendizado de Máquina
19.
Biointerphases ; 19(1)2024 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-38407470

RESUMO

SU-8 is an epoxy-based, biocompatible thermosetting polymer, which has been utilized mainly to fabricate biomedical devices and scaffolds. In this study, thin, single-layered, freestanding tuneable porous SU-8 membranes were microfabricated and surface hydrophilized for efficient bioseparation. Unlike the previous thicker membranes of 200-300 µm, these thin SU-8 membranes of 50-60 µm thickness and pores with 6-10 µm diameter were fabricated and tested for blood-plasma separation, without any additional support structure. The method is based on making a patterned SU-8 layer by electrospin coating and UV lithography on a sacrificial polyethylene terephthalate (PET) sheet attached to a silicon wafer. Poor adhesion between PET and SU-8 aid in the convenient release of the thin porous membranes with uniform pore formation. The single-layered self-supporting membranes were strong, safe, sterilizable, reusable, and suitable for plasma separation and postfermentation broth enrichment.


Assuntos
Plasma , Polímeros , Porosidade , Silício
20.
Mar Drugs ; 22(2)2024 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-38393067

RESUMO

The limited availability of phosphate, nitrogen and silicon in the growth media affects the growth, cellular processes, and metabolism of diatoms. Silicon deficiency primarily affects diatom morphology, while phosphate deficiency reduces the production of nucleic acids and phospholipids. Differences in pigment and protein composition are mainly due to nitrogen deficiency. In this study, Chaetoceros socialis and Chaetoceros costatus were cultured under phosphate, nitrogen, and silicon deprivation conditions. The diatom biomass was collected during the stationary growth phase and extracted with 70% ethanol under ultrasonication. The chemical profiles of the extracts were analyzed by high-performance liquid chromatography with high-resolution mass spectrometry with electrospray ionisation (UHPLC-ESI-HRMS), while the antioxidant capacity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Pigments, fatty acids, sterols, and derivatives were detected in both species. The total phenolic content in the extracts ranged from 46.25 ± 1.08 to 89.38 ± 6.21 mg of gallic acid equivalent (GAE)/L and from 29.58 ± 1.08 to 54.17 ± 1.18 mg GAE/L. for C. costatus and C. socialis, respectively. Antioxidant activity was higher in C. costatus extracts, especially those obtained from nitrogen-deprived media. The results of this study contribute to the existing knowledge and the ongoing efforts to overcome application and commercialization barriers of microalgae for wide-ranging potential in different industries.


Assuntos
Antioxidantes , Diatomáceas , Antioxidantes/química , Silício , Extratos Vegetais/química , Nutrientes , Nitrogênio/análise , Fosfatos
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