Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 34
Filtrar
1.
PLoS One ; 18(5): e0285470, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37220121

RESUMO

Presently, the Strait of Gibraltar is undergoing an unprecedented invasion of the alien alga Rugulopteryx okamurae of North Pacific origin. According to the scarce literature, the algae first settled in the south shore, probably following commercial exchanges with French ports where it was accidentally introduced together with Japanese oysters imported for mariculture. There is no certainty, however, that the algae first colonized the south shore of the Strait and, from there, spread to the north. It could well have been the opposite. Whatever the case, it spread all over the Strait and surrounding areas with amazing rapidity. Human-mediated vectors (algae attached to ship hulls or fishing nets, for example) can be behind the spread from the shore initially settled to the algae-free shore on the opposite side. But it could also have happened by means of hydrodynamic processes without direct human intervention. This possibility is assessed in this paper by revisiting historical current meter profiles collected in the Strait of Gibraltar searching for secondary cross-strait flows. All the stations present an intermediate layer of northward cross-strait velocity near the interface of the mean baroclinic exchange along with a surface layer above of southward velocity, whose lower part also overlaps the interface zone. The first one would back the south-to-north transport of algal fragments, the second one, the north-to south. In both cases, algae must reach the depth of the interface. The vertical velocity field in the area, which far exceeds the small sedimentation velocity of the algae, allows their vertical displacements throughout the water column. Its endurance to survive under the weak or no light conditions that will prevail during the cross-strait transport and its capability of reactivating the metabolism after this unfavorable period, offers chances for colonizing the opposite shore. Therefore, the propagation of the algae by hydrodynamic processes, without human intervention, cannot be ruled out.


Assuntos
Espécies Introduzidas , Feófitas , Gibraltar
2.
Braz. j. biol ; 83: 1-10, 2023. map, ilus, tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1468982

RESUMO

Seaweeds are a major marine resource that can be explored to develop novel pharmaceutical molecules. The present study showed the presence of unique bioactive components in the petroleum ether extract (PEE) and methanolic extract (ME) of Sargassum tenerrimum. The gas chromatography-mass spectrometry analysis suggested that the PEE of S. tenerrimum contained antibacterial biomolecules: hexadecanoic acid, methyl ester, 17-pentatriacontene, dasycarpidan-1-methanol, and acetate (ester). However, the ME of S. tenerrimum exhibited better antibacterial effect than the PEE due to the presence of the bioactive compounds 1,2-benzenedicarboxylic acid, diisooctyl ester, tetratetracontane, 1-docosene, 1,2-benzenediol, and benzoic acid. Thus, promising antibacterial molecules can be isolated from S. tenerrimum for better therapeutic use.


As algas marinhas são um importante recurso marinho que pode ser explorado para desenvolver novas moléculas farmacêuticas. O presente estudo mostrou a presença de componentes bioativos únicos no extrato etéreo de petróleo (PEE) e no extrato metanólico (ME) de Sargassum tenerrimum. A análise por cromatografia gasosa espectrometria de massa sugeriu que o PEE de S. tenerrimum continha biomoléculas antibacterianas: ácido hexadecanoico, éster metílico, 17-pentatriaconteno, dasycarpidan-1-metanol e acetato (éster). Entretanto, o ME de S. tenerrimum exibiu melhor efeito antibacteriano do que o PEE devido à presença dos compostos bioativos ácido 1,2-benzenodicarboxílico, éster diisooctil, tetratetracontano, 1-docosene, 1,2-benzoenodiol e ácido benzoico. Assim, moléculas antibacterianas promissoras podem ser isoladas de S. tenerrimum para melhor uso terapêutico.


Assuntos
Antibacterianos/análise , Cromatografia Gasosa-Espectrometria de Massas , Feófitas/química , Sargassum/química
3.
Artigo em Inglês | BBO - Odontologia, LILACS | ID: biblio-1135509

RESUMO

Abstract Objective: To determine the total level of flavonoids in brown algae extract Padina sp., Sargassum sp., and Turbinaria sp., which could serve as an analgesic and anti-inflammatory drug. Material and Methods: This is an experimental study with a one-shot case study research design. The study sample consisted of three species of brown algae, namely, Padina sp., Sargassum sp., and Turbinaria sp. The study samples were obtained from Saugi Island, Pangkep, Regency. The sampling method used was convenience sampling. The total flavonoid level in the three extracts of brown algae samples was determined at three concentrations (150 ppm, 300 ppm, and 450 ppm) with three replicates. The analysis used a colorimetric method, a spectrophotometer and aluminium chloride as the reagent. Results: The total level of flavonoids in Padina sp. was the highest at 0.894 ± 0.027%, compared to the levels of 0.786 ± 0.075% in Sargassum sp. and 0.745 ± 0.016% in Turbinaria sp. Conclusion: Padina sp. had the highest total flavonoid levels compared to Sargassum sp. and Turbinaria sp. Flavonoid compounds from brown algae have the potential to be used as analgesic and anti-inflammatory drugs.


Assuntos
Alga Marinha , Flavonoides , Feófitas , Compostos Fitoquímicos , Anti-Inflamatórios/uso terapêutico , Projetos de Pesquisa , Preparações Farmacêuticas , Espectrofotômetros/métodos , Estudos de Intervenção , Estatísticas não Paramétricas , Sargassum , Indonésia/epidemiologia
5.
Int. j. morphol ; 36(3): 979-983, Sept. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-954218

RESUMO

Turbinaria deccurrens Bory contains bioactive compound that is beneficial for health. Turbinaria deccurrens Bory is one of many species of brown seaweed that grows in Indonesian marine life and has been known to have cytotoxic activity. The aim of this study is to determine fucoxantin content and the cytotoxic activity of extract and fraction T. decurrens on colon cancer cell lines. Cytotoxic assay of ethanolic extract, n-hexane, ethyl acetate and ethanolic fractions against HCT-116 by MTS assay using Cell Counting Kit-8 (CCK-8). Fucoxantin content in extract and fraction were analyzed using Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) analysis. Extract and fraction of T. decurrens contain fucoxanthin with the highest content of fucoxanthin was in ethyl acetate fraction. CCK-8 assay showed that extract, n-hexane and ethyl acetate fraction inhibited the growth of HCT-116. Brown seaweed Turbinaria decurrens was potential as an anticolon cancer agent.


Turbinaria deccurrens Bory contiene compuestos bioactivos que son beneficiosos para la salud. Turbinaria deccurrens Bory es una de muchas especies de algas pardas que crecen en aguas marinas de Indonesia y se ha estudiado su actividad citotóxica. El objetivo de este estudio fue determinar el contenido de fucoxantina y la actividad citotóxica del extracto y la fracción de T. decurrens en líneas celulares de cáncer de colon. Se llevó a cabo un ensayo citotóxico de extracto etanólico, nhexano, acetato de etilo y fracciones etanólicas contra HCT-116 mediante ensayo MTS utilizando Cell Counting Kit-8 (CCK-8). El contenido de fucoxantina en el extracto y la fracción se analizaron usando cromatografía líquida de alta resolución de fase reversa (RP-HPLC). El extracto y la fracción de T. decurrens contienen fucoxantina conmayor contenido de fucoxantina en la fracción de acetato de etilo. El ensayo CCK-8 mostró que la fracción de extracto, n-hexano y acetato de etilo inhibía el crecimiento de HCT-116. El alga marrón Turbinaria decurrens es un agente potencial contra el cáncer de colon.


Assuntos
Extratos Vegetais/administração & dosagem , Neoplasias do Colo/tratamento farmacológico , Xantofilas/administração & dosagem , Células HCT116/efeitos dos fármacos , Feófitas , Extratos Vegetais/química , Xantofilas/análise , Linhagem Celular Tumoral/efeitos dos fármacos
6.
Electron. j. biotechnol ; 28: 27-34, July. 2017. tab, ilus, graf
Artigo em Inglês | LILACS | ID: biblio-1015826

RESUMO

Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Alginate is the principal component of the exopolysaccharide matrix in biofilms produced by Pseudomonas, and this component is highly demanded for the production of a wide variety of commercial products. There is a constant search for efficient alginate-producing organisms. Results: In this study, a novel strain of Pseudomonas mandelii isolated from Antarctica was characterized and found to overproduce alginate compared with other good alginate producers such as Pseudomonas aeruginosa and Pseudomonas fluorescens. Alginate production and expression levels of the alginate operon were highest at 4°C. It is probable that this alginate-overproducing phenotype was the result of downregulated MucA, an anti-sigma factor of AlgU. Conclusion: Because biofilm formation is an efficient bacterial strategy to overcome stressful conditions, alginate overproduction might represent the best solution for the successful adaptation of P. mandelii to the extreme temperatures of the Antarctic. Through additional research, it is possible that this novel P. mandelii strain could become an additional source for biotechnological alginate production.


Assuntos
Pseudomonas/metabolismo , Alginatos/metabolismo , Polissacarídeos Bacterianos/metabolismo , Pseudomonas/crescimento & desenvolvimento , Pseudomonas/genética , Adaptação Biológica , Temperatura Baixa , Microscopia Confocal , Biofilmes , Feófitas , Tipagem de Sequências Multilocus , Reação em Cadeia da Polimerase em Tempo Real , Regiões Antárticas
7.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-285247

RESUMO

Brown algae are well known as a source of biologically active compounds, especially those having antioxidant activities, such as phlorotannins. In this study we examined the antioxidant activities of crude phlorotannins extracts (CPEs) obtained from Sargassum hemiphyllum (SH) and fractionated according to the molecular weights. When CPEs were administrated at a dose of 30 mg/kg to Kunming mice pre-treated with carbon tetrachloride (CCl4), the levels of oxidative stress indicators in the liver, kidney and brain were significantly reduced in vivo. All the components of various molecular weight fractions of CPEs exhibited greater scavenging capacities in clearing hydroxyl free radical and superoxide anion than the positive controls gallic acid, vitamin C and vitamin E. Particularly, the components greater than 30 kD obtained from ethyl acetate phase showed the highest antioxidant capacities. These results indicated that SH is a potential source for extracting phlorotannins, the algal antioxidant compounds.


Assuntos
Animais , Masculino , Camundongos , Antioxidantes , Farmacologia , Ácido Ascórbico , Farmacologia , Encéfalo , Metabolismo , Patologia , Tetracloreto de Carbono , Toxicidade , Intoxicação por Tetracloreto de Carbono , Tratamento Farmacológico , Metabolismo , Patologia , Fracionamento Químico , Métodos , Ácido Gálico , Farmacologia , Radical Hidroxila , Metabolismo , Rim , Metabolismo , Patologia , Extração Líquido-Líquido , Métodos , Fígado , Metabolismo , Patologia , Camundongos Endogâmicos , Oxirredução , Estresse Oxidativo , Feófitas , Química , Sargassum , Química , Superóxidos , Metabolismo , Taninos , Farmacologia , Vitamina E , Farmacologia
8.
Artigo em Coreano | WPRIM (Pacífico Ocidental) | ID: wpr-30550

RESUMO

When exposed to gamma-rays, hair follicular cells immediately go through apoptosis, which hampers their rapid differentiation essential for the regeneration of hair. Phloroglucinol (PG) is a phenolic compound of Ecklonia cava, brown algae abundant in Jeju island, Korea. Containing plentiful polyphenols, PG is known for its instructive effects by inhibiting apoptosis, scavenging oxygen radicals, and protecting cells against oxidative stress. In this study, we demonstrate that PG rescues radiosensitive hair follicular cells from gamma radiation-induced apoptosis and DNA damage. To identify protective capacity of PG on hair follicles, we irradiated with 8.5 Gy (1.5 Gy/min) of gamma-rays to the whole body of C57BL/6 mice at day 6 after depilation with or without PG. In mice exposed to radiation, the expression of proapoptotic molecule p53 was downregulated in the skin of PG treated group. On immunohistochemical observation of the skin, PG inhibited the immunoreactivity of p53 and cleaved caspase-3. PG treatment protected hair follicular cells from cell death due to gamma-radiation. Our results suggest that PG presents radioprotective effects by inhibiting apoptosis of radiosensitive hair follicular cells and can protect hair follicular cells from gamma-ray induced damage.


Assuntos
Animais , Camundongos , Apoptose , Caspase 3 , Morte Celular , Dano ao DNA , Folículo Piloso , Remoção de Cabelo , Cabelo , Coreia (Geográfico) , Estresse Oxidativo , Feófitas , Fenol , Floroglucinol , Polifenóis , Espécies Reativas de Oxigênio , Regeneração , Pele
9.
Hist. ciênc. saúde-Manguinhos ; 22(2): 391-409, Apr-Jun/2015.
Artigo em Espanhol | LILACS | ID: lil-747128

RESUMO

Este trabajo aborda las principales características del surgimiento y primeros diez años de funcionamiento de los Centros de Higiene Infantil, establecimientos dependientes del Departamento de Salubridad Pública desde 1922 en la Ciudad de México y que tuvieron como objetivo atender a las madres, desde el periodo de gestación, y a los niños, desde el nacimiento hasta los dos años de edad. Revisa las acciones que dieron origen a este proyecto y su consolidación. Analiza la estructura de estos Centros, las características de las madres y los niños que atendieron, así como las funciones que realizaron médicos y enfermeras enfatizando la noción de prevención de las enfermedades infantiles, para finalmente mostrar un primer balance acerca de los alcances y limitaciones que tuvieron.


This article deals with the main features of the emergence and first ten years of the Centros de Higiene Infantil, facilities run by the Departamento de Salubridad Pública from 1922 on in Mexico City with the goal of providing care for mothers from pregnancy onwards and children from birth to two years of age. It reviews the actions that gave rise to this project and how it became established. It analyzes the structure of these centers, the characteristics of the mothers and children seen there and the functions performed by doctors and nurses, stressing the notion of preventing childhood illnesses, and ends with a first assessment of the effects and limitations of these centers.


Assuntos
Humanos , Cloretos/química , Compostos de Ouro/química , Ouro/química , Nanopartículas Metálicas/química , Oxirredução , Tamanho da Partícula , Feófitas
10.
Natural Product Sciences ; : 205-209, 2015.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-221415

RESUMO

Fucoidan, a sulfated polysaccharide is found in several types of edible brown algae. It has shown numerous biological activities; however, the molecular mechanisms on the activity against atopic dermatitis have not been reported yet. We now examined the effects of fucoidan on chemokine production co-induced by TNF-alpha/IFN-gamma, and the possible mechanisms underlying these biological effects. Our data showed that fucoidan inhibited the TNF-alpha/IFN-gamma-induced production of thymus and activation-regulated chemokine (TARC) and macrophagederived chemokine (MDC) mRNA in human keratinocytes HaCaT cells. Also, fucoidan suppressed phosphorylation of nuclear factor kappa B (NF-kappaB) and activation of signal transducer and activator of transcription (STAT)1 in a dose-dependent manner. In addition, fucoidan significantly inhibited activation of extracellular-signal-regulated kinases (ERK) phosphorylation. These data indicate that fucoidan shows anti-inflammatory effects by suppressing the expression of TNF-alpha/IFN-gamma-induced chemokines by blocking NF-kappaB, STAT1, and ERK1/2 activation, suggestive of as used as a therapeutic application in inflammatory skin diseases, such as atopic dermatitis.


Assuntos
Humanos , Quimiocina CCL17 , Quimiocinas , Dermatite Atópica , Queratinócitos , NF-kappa B , Feófitas , Fosforilação , Fosfotransferases , RNA Mensageiro , Dermatopatias , Fator de Transcrição STAT1 , Transdutores
11.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-727829

RESUMO

Fucoidan, a fucose-rich sulfated polysaccharide derived from brown seaweed in the class Phaeophyceae, has been widely studied for its possible health benefits. However, the potential of fucoidan as a possible treatment for hyperpigmentation is not fully understood. This study investigated the effects of fucoidan on melanogenesis and related signaling pathways using Mel-Ab cells. Fucoidan significantly decreased melanin content. While fucoidan treatment decreased tyrosinase activity, it did not do so directly. Western blot analysis indicated that fucoidan downregulated microphthalmia-associated transcription factor and reduced tyrosinase protein expression. Further investigation showed that fucoidan activated the extracellular signal-regulated kinase (ERK) pathway, suggesting a possible mechanism for the inhibition of melanin synthesis. Treatment with PD98059, a specific ERK inhibitor, resulted in the recovery of melanin production. Taken together, these findings suggest that fucoidan inhibits melanogenesis via ERK phosphorylation.


Assuntos
Western Blotting , Hiperpigmentação , Benefícios do Seguro , Melaninas , Fator de Transcrição Associado à Microftalmia , Monofenol Mono-Oxigenase , Feófitas , Fosforilação , Fosfotransferases , Alga Marinha
12.
Electron. j. biotechnol ; 16(3): 6-6, May 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-684004

RESUMO

Background: The increased industrial activity has resulted in the discharge of large amount of pollutants including non-degradable metals into the environment. Chromium is produced in several industrial processes and it can be found in the environment in two stable oxidation states, Cr(VI) and Cr(III). Cr(VI) is more hazardous due to its carcinogenic and mutagenic effects on living organisms. Although much less toxic, Cr(III) can also exert genotoxic effects under prolonged or severe exposure. It can be separated from the solution by precipitation but biosorption using brown algae seems to be an effective and sustainable treatment technique owing to its cost-effectiveness and environmental friendly characteristics. Macrocystis pyrifera and Undaria pinnatifida are two marine brown macroalgae with high capability of removing heavy metals including Cr(III) in batch mode of operation. In this work packed bed biosorption of Cr(III) by M. pyrifera and U. pinnatifida biomasses was evaluated. Results: The shapes of the breakthrough curves were rather different for each biomaterial. Parameters like the breakthrough time (t b) andzone mass transfer (MTZ) showed that U. pinnatifida has greater affinity for Cr(III). The maximum adsorption capacity at the exhaustion operating time (t e) demonstrated that M. pyrifera has higher retention capacity of Cr(III). The experimental data were fitted to Thomas, Yoon-Nelson and Dose-Response models. The best correlation coefficient (0.94 or 0.96) was obtained with Dose-Response that accurately describes the uptake behaviour of Cr(III) on the seaweed biomasses under different experimental conditions. The FT-IR spectra evidenced that Cr(III) adsorption occurred mainly by interaction between metal and carboxylate groups present on both the seaweed surfaces. Conclusions: M. pyrifera and U. pinnatifida biomasses are efficient biosorbents for Cr(III) adsorption under a continuous mode of operation although differences between uptake capacities suggest different mechanisms involved in the biosorption.


Assuntos
Metais Pesados/metabolismo , Macrocystis/metabolismo , Undaria/metabolismo , Poluentes Industriais , Cromo/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Biomassa , Adsorção , Feófitas
13.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-312475

RESUMO

<p><b>OBJECTIVE</b>To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp. [Turbinaria conoides (T. conoides) and Turbinaria ornata (T. ornata) collected from Gulf of Mannar of southeastern coast of India in various in vitro systems.</p><p><b>METHODS</b>The antioxidant activity was evaluated using different in vitro systems, viz., 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2'-azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium salt (ABTS), H2O2/HO radical scavenging, Fe(2+) ion chelating ability, and reducing potential. Folin-Ciocalteu method was used to determine the total phenolic content of the extracts, and the results were expressed as mg of gallic acid equivalents (GE)/g of the seaweed extracts. Thiobarbituric acid-reactive substances assay was employed to assess the ability of the seaweed extracts to inhibit lipid oxidation.</p><p><b>RESULTS</b>Ethyl acetate (EtOAc) fraction of T. conoides registered significantly higher phenolic content (105.97 mg GE/g) than that of T. ornata (69.63 mg GE/g). Significantly higher antioxidant potential as determined by DPPH (64.14%) radical scavenging activity was registered in EtOAc fraction of T. ornata. A higher ABTS(•+) radical scavenging (IC50 3.16 µg/mL), Fe(2+) chelating (IC50 0.46 mg/mL), H2O2 scavenging (IC50 4.25 mg/mL), lipid peroxidation inhibitory (TBARS, IC50 0.21 mg/mL), and reducing abilities (IC50 52.67 mg/mL) (P<0.05) were realized in EtOAc fraction of T. ornata than other fractions.</p><p><b>CONCLUSIONS</b>This study indicated the potential use of T. conoides and T. ornata as candidate species to be used as food supplements/functional foods to increase shelf-life of food items for human consumption, and nutraceuticals to deter deleterious free radical-induced life-threatening diseases.</p>


Assuntos
Antioxidantes , Química , Índia , Feófitas , Química , Fenóis , Extratos Vegetais , Química , Especificidade da Espécie
15.
Chinese Journal of Biotechnology ; (12): 838-845, 2011.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-324495

RESUMO

Marine can be considered as a rather unexplored source of biological material. Production of algal oligosaccharides by using valuable enzymes from marine origin has become an important way to utilize marine resources. As one of algal tool enzymes, the use of alginate lyases has been focused mainly on development and application of alginate oligosaccharides with bioactive function in recent years. In this paper, we reviewed the research of alginate lyases over the past decade in several aspects, including their origin, diversity, substrate specification, mode of action, structure and catalysis mechanism, assay of enzyme activity, enzyme characterization, as well as our own experience on this subject. At the end of the review, the application prospects of alginate lyases are presented.


Assuntos
Alginatos , Metabolismo , Ácido Glucurônico , Metabolismo , Ácidos Hexurônicos , Metabolismo , Biologia Marinha , Métodos , Oligossacarídeos , Metabolismo , Feófitas , Polissacarídeo-Liases , Classificação , Metabolismo , Especificidade por Substrato
16.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-727373

RESUMO

Fucoidan is a sulfated polysaccharide derived from brown algae that has been reported to perform multiple biological activities, including immunostimulation. In this study, we investigated whether fucoidan has beneficial effects on endotoxemia induced by LPS, a septic model in mice. The focus of this study was on survival rates and spleen function of the mice upon treatment. We found that fucoidan had prophylactic effects on the survival rate of mice with endotoxemia. Flow cytometric analysis using antibodies for subset-specific markers revealed that fucoidan profoundly reversed the depleted population of dendritic cells in mice with endotoxemia. According to Western blot analysis, the spleen cells of LPS/fucoidan-treated mice showed a higher expression of anti-apoptotic molecules compared to those of LPS-treated mice. Also, fucoidan-treated spleen cells were more responsive to mitogens. Taken together, these results demonstrate that fucoidan pre-treatment has beneficial effects on the survival rate and function of the spleen in mice with endotoxemia. This study may broaden the use of fucoidan in clinical fields, especially endotoxemia.


Assuntos
Animais , Camundongos , Anticorpos , Western Blotting , Células Dendríticas , Endotoxemia , Imunização , Mitógenos , Feófitas , Polissacarídeos , Baço , Taxa de Sobrevida
17.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-164299

RESUMO

In this study, antioxidant and free radical scavenging activities of the natural antioxidative compound, pyrogallol-phloroglucinol-6,6'-bieckol (PPB) isolated from brown algae, Ecklonia cava was assessed in vitro by measuring the radical scavenging activities (DPPH, alkyl, hydroxyl, and superoxide) using electron spin resonance (ESR) spectrometry, intracellular reactive oxygen species (ROS) scavenging activity, and DNA damage assay. According to the results of these experiments, the scavenging activity PPB against difference radicals was in the following order: DPPH, alkyl, hydroxyl, and superoxide radicals (IC50; 0.90, 2.54, 62.93 and 109.05 microM). The antioxidant activities of PPB were higher than that of the commercial antioxidant, ascorbic acid. Furthermore, PPB effectively inhibited DNA damage induced by H2O2. These results suggest that the natural antioxidative compound, PPB, can be used by the natural food industry.


Assuntos
Ácido Ascórbico , Dano ao DNA , Espectroscopia de Ressonância de Spin Eletrônica , Indústria Alimentícia , Feófitas , Espécies Reativas de Oxigênio , Análise Espectral , Superóxidos
18.
Biocell ; 34(1): 45-52, Apr. 2010. ilus
Artigo em Inglês | LILACS | ID: lil-595049

RESUMO

The present paper deals with the ultrastructure of zoospores produced by the plasmodiophorid Maullinia ectocarpii, living in the marine algal host Ectocarpus siliculosus. The zoospores described here are very similar to secondary zoospores of Polymyxa graminis and Phagomyxa sp. (the latter an algal endoparasite, also). Our results indicate that M. ectocarpii produces two types of plasmodia, and suggest that is a species with a complete life cycle, as it is known for all the Plasmodiophormycota that have been studied. Sporogenic and sporangial plasmodia produce, respectively, primary zoospores with parallel flagella within thick walled resting sporangia, and secondary zoospores with opposite flagella within thin walled sporangia.


Assuntos
Esporos de Protozoários/ultraestrutura , Flagelos/ultraestrutura , Feófitas/parasitologia , Microscopia Eletrônica de Transmissão
19.
Biocell ; 34(1): 45-52, Apr. 2010. ilus
Artigo em Inglês | BINACIS | ID: bin-127229

RESUMO

The present paper deals with the ultrastructure of zoospores produced by the plasmodiophorid Maullinia ectocarpii, living in the marine algal host Ectocarpus siliculosus. The zoospores described here are very similar to secondary zoospores of Polymyxa graminis and Phagomyxa sp. (the latter an algal endoparasite, also). Our results indicate that M. ectocarpii produces two types of plasmodia, and suggest that is a species with a complete life cycle, as it is known for all the Plasmodiophormycota that have been studied. Sporogenic and sporangial plasmodia produce, respectively, primary zoospores with parallel flagella within thick walled resting sporangia, and secondary zoospores with opposite flagella within thin walled sporangia.(AU)


Assuntos
Flagelos/ultraestrutura , Feófitas/parasitologia , Esporos de Protozoários/ultraestrutura , Microscopia Eletrônica de Transmissão
20.
Biol. Res ; 43(1): 31-37, 2010. ilus, tab
Artigo em Inglês | LILACS | ID: lil-548027

RESUMO

The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70 percent) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in different cell lines including HT-29, Caco-2, T47D, MDA-MB468 and NIH 3T3 cell lines by MTT and AnnexinV-PI assay. The hexan fraction of S. swartzii and C. myrica showed selective cytotoxicity against proliferation of Caco-2 cells (IC50<100 μg/ml) T47D cell line (IC50<100 μg/ml), respectively. S. swartzii and C. myrica were also observed for increasing apoptosis in Caco-2 and T47D cells. Total extract and fractions of C. sinuosa did not show any significant cytotoxicity against the studied cell lines. MDA-MB468 cells were more sensitive to C. myrica than was T47D (IC50 99.9±8.11 vs. 56.50‘± 0.88). This reflects an estrogen receptor independent mechanism for cytotoxicity of the extract. The IC50 of the hexan fraction of C. myrica on T47D parent cells was lower than it was on T47D-TR cells (IC50 99.9±8.11 vs. 143.15 ± 7.80). This finding suggests a role for the MDR-1 in the development of possible future tolerance to the extract.


Assuntos
Humanos , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Feófitas/química , Linhagem Celular Tumoral , Ensaio Cometa , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Feófitas/classificação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...