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1.
Braz. j. oral sci ; 8(2): 84-87, Apr.-June 2009. tab
Artigo em Inglês | LILACS, BBO - Odontologia | ID: lil-556470

RESUMO

Aim: To assess the cytotoxicity of polycarbonate orthodontic brackets. Methods: Polycarbonate brackets from two different manufacturers, namely, Composite bracket (Morelli™) and Silkon Plus bracket (American Orthodontics™), were assessed. In addition to these two experimental groups, other three control groups were included: Positive Control Group (C+) consisting of amalgam cylinders, Negative Control Group (C-) consisting of glass rods, and Cell Control Group (CC) consisting of cells not exposed to any material. All brackets were previously sterilized under ultra-violet light (UV) and, then, immersed in Eagle’s minimum essential media (MEM) for 24 hours, after which the supernatants were removed and placed into contact with L929 fibroblast cells. Cytotoxicity was evaluated at 24, 48, 72 and 168 hours. After contact with MEM, the cells were further incubated at 37oC for 24 hours and 100 mL of 0.01% neutral red dye were added. The cells were incubated again at 37ºC for three hours to incorporate the dye. After this period, the cells were fixed and viable cell counting was performed by spectrophotometry at 492 nm wavelength. Results: No statistically significant difference was found between the experimental groups (1 and 2) and the negative and cell control groups (p > 0.05). The Positive Control Group exhibited high cytotoxicity throughout experimental period are differed significantly from the other groups (p < 0.05). Conclusions: Polycarbonate orthodontic brackets were found not to be cytotoxic within the evaluated experimental period.


Assuntos
Carbonatos/toxicidade , Braquetes Ortodônticos , Materiais Biocompatíveis , Células Cultivadas , Fibroblastos , Teste de Materiais , Fatores de Tempo
2.
Braz. j. oral sci ; 82(2): 84-87, Apr.-June 2009. tab
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-850548

RESUMO

Aim: To assess the cytotoxicity of polycarbonate orthodontic brackets. Methods: Polycarbonate brackets from two different manufacturers, namely, Composite bracket (Morelli™) and Silkon Plus bracket (American Orthodontics™), were assessed. In addition to these two experimental groups, other three control groups were included: Positive Control Group (C+) consisting of amalgam cylinders, Negative Control Group (C-) consisting of glass rods, and Cell Control Group (CC) consisting of cells not exposed to any material. All brackets were previously sterilized under ultra-violet light (UV) and, then, immersed in Eagle’s minimum essential media (MEM) for 24 hours, after which the supernatants were removed and placed into contact with L929 fibroblast cells. Cytotoxicity was evaluated at 24, 48, 72 and 168 hours. After contact with MEM, the cells were further incubated at 37oC for 24 hours and 100 mL of 0.01% neutral red dye were added. The cells were incubated again at 37oC for three hours to incorporate the dye. After this period, the cells were fixed and viable cell counting was performed by spectrophotometry at 492 nm wavelength. Results: No statistically significant difference was found between the experimental groups (1 and 2) and the negative and cell control groups (p > 0.05). The Positive Control Group exhibited high cytotoxicity throughout experimental period are differed significantly from the other groups (p < 0.05). Conclusions: Polycarbonate orthodontic brackets were found not to be cytotoxic within the evaluated experimental period.


Assuntos
Carbonatos/toxicidade , Braquetes Ortodônticos , Materiais Biocompatíveis , Células Cultivadas , Fibroblastos , Teste de Materiais , Fatores de Tempo
3.
Rev. odontol. Univ. Säo Paulo ; 1(1): 3-7, jan.-mar. 1987. tab
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-57730

RESUMO

Foi estudado por meio da titulometria a perda do teor de cloro da soluçäo de Dakin quando estocada em vidro ambar, mantida à temperatura ambiente, exposta à luz solar e na geladeira (9-C). Verificou-se que a soluçäo de Dakin quando exposta à luz solar perdeu 79,11 por cento do teor de cloro ativo em 122 dias, à temperatura ambiente a perda teor de cloro foi de 62,69 por cento e quando armazenada na geladeira, ou seja, a 9 graus centígrados a perda foi somente de 22,39 por cento no mesmo período


Assuntos
Ácidos Bóricos/metabolismo , Cálcio/metabolismo , Carbonatos/metabolismo , Cloro/metabolismo , Armazenamento de Medicamentos , Gelo , Luz Solar , Temperatura
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