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1.
Endocrinol. diabetes nutr. (Ed. impr.) ; 67(3): 186-193, mar. 2020. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-188147

RESUMO

Introducción: Evaluar la seguridad y eficacia de un extracto de aceite de rosa mosqueta en la prevención y tratamiento de las lesiones cutáneas en las manos de los pacientes con diabetes mellitus tipo 1 (DMT1) secundarias a las punciones digitales para el control glucémico. Pacientes y métodos: Estudio prospectivo, aleatorizado, controlado, abierto, con evaluadores ciegos e intervencionista en pacientes de edades entre 6 y 17 años con DMT1 y control intensivo de la glucemia con ≥ 7 punciones capilares diarias durante 12 días. Se evaluaron 3 variables principales (eritema, engrosamiento cutáneo, pérdida de la integridad cutánea) de la siguiente forma: 0: ausente, 1: leve, 2: moderado, 3: intenso. El estudio fue aprobado por el Comité Ético del hospital. Resultados: Se incluyó a 68 niños, por tanto, 136 manos: 80 recibieron aceite de rosa mosqueta y 56 fueron controles. Las características basales de los 2 grupos fueron similares. El 76,3% y el 78,6% presentaban alguna lesión dermatológica inicial, respectivamente. La mediana de valoración global final fue de 0,10 (0,03; 0,30) y de 0,06 (0,00; 0,23), en el grupo de aceite de rosa mosqueta y grupo control, respectivamente. Se encontró una mejoría estadísticamente significativa de la valoración global solo en el grupo control (p = 0,049). No se encontraron diferencias estadísticamente significativas para la comparación de medianas del resto de las variables principales. No se registraron efectos adversos. Conclusión: Se encontró una alta frecuencia de lesiones dermatológicas secundarias a punciones capilares digitales, la mayoría de las cuales fueron lesiones leves. La aplicación de aceite de rosa mosqueta fue segura y no supuso una mejoría en las lesiones dermatológicas


Introduction: This study was intended to assess the efficacy and safety of a rosehip seed oil (RHO) extract in the prevention and treatment of skin lesions in the hands of patients with type 1 diabetes mellitus (T1DM) caused by finger prick blood glucose monitoring. Patients and method: A prospective, randomized, controlled, open-label, rater-blinded trial in patients aged 6-17 years with T1DM and intensive blood glucose control (≥ 7 finger pricks daily) for 12 days. Three main variables (erythema, skin thickening, and loss of skin integrity) were assessed using a scale ranging from 0 (absent) to 3 (severe involvement). The study was approved by the ethics committee of the hospital. Results: Sixty-eight children, and thus 136 hands, were included; 80 hands received rosehip seed oil and 56 hands acted as controls. Baseline characteristics of both groups were similar, with 76.3% and 78.6% of the hands respectively showing skin lesions at study start. Median final global assessment was 0.10 (0.03; 0.30) in the group that received rosehip seed oil and 0.06 (0.00; 0.23) in the control group. A statistically significant improvement in global assessment was found in the control group (P=0.049). No significant differences were found when the medians of the other main variables were compared. No adverse effects were recorded. Conclusion: A high prevalence of skin lesions secondary to finger prick glucose monitoring, most of them mild lesions, was found at study start. Treatment with rosehip seed oil was safe and was not effective for improving skin lesions


Assuntos
Humanos , Criança , Adolescente , Masculino , Feminino , Resultado do Tratamento , Rosa , Capilares/lesões , Traumatismos dos Dedos/terapia , Extratos Vegetais/uso terapêutico , Técnica Clamp de Glucose/métodos , Índice Glicêmico , Diabetes Mellitus Tipo 1/diagnóstico , Estudos Prospectivos , Eritema/terapia , Segurança do Paciente
3.
Eur. j. anat ; 23(4): 267-272, jul. 2019. ilus
Artigo em Inglês | IBECS | ID: ibc-183000

RESUMO

Ayurvedic medicines is known to use heavy metals in their preparation. Nagabhasma is one such form of a lead-based medicine. Even though lead is known to be toxic to several systems of the human body, according to Ayurveda, the metallic toxicity of the lead gets nullified and thereby imbibes medicinal property when it is prepared using many herbs and stringent traditional methods. Therefore, the present study is designed to evaluate the effect of such detoxified lead in various stages of authentically prepared Nagabhasma on the histopathology of liver in comparison with lead acetate and commercially available Nagabhasma-administered animals. Less than the human-equivalent doses of Nagabhasma at four intermittent stages of its preparation were administered orally for 30 days and 60 days (short term and long term exposure) to Wistar rats. In another set of experiment, test-material-administered animals were kept under observation for an additional period of two months to record the residual effect. Immediately after the administration and after the observation period, the animals were sacrificed to collect the liver for histopathological examination. The histopathological results of the immediate and residual effects showed varying alterations in the microarchitecture of the liver as the stages of Nagabhasma preparation advanced. The final product (stage 4 bhasma), showed very less toxic effect in comparison with other stages. In conclusion, the results state that, by following the traditional procedures while preparing Nagabhasma, the metallic lead gets converted into non-toxic organometallic compound


No disponible


Assuntos
Humanos , Animais , Metais Pesados/toxicidade , Medicina Ayurvédica/efeitos adversos , Fígado/efeitos dos fármacos , Chumbo/toxicidade , Hepatócitos/efeitos dos fármacos , Capilares/efeitos dos fármacos , Fígado/anatomia & histologia , Fígado/patologia , Ratos Wistar , Vasos Sanguíneos/anatomia & histologia , Vasos Sanguíneos/patologia
4.
Rev. esp. enferm. dig ; 111(3): 214-222, mar. 2019. ilus
Artigo em Inglês | IBECS | ID: ibc-189828

RESUMO

Background and aims: pathological angiogenesis plays an important role in the progression of chronic liver diseases. Asparaginyl endopeptidase (AEP) participates in tumor angiogenesis and was recently shown to be associated with liver fibrosis. This study aimed to explore the effect of AEP on liver sinusoidal endothelial cell (LSECs) angiogenesis and determine the underlying mechanism. Methods: cultured LSECs were infected with lentiviruses in order to suppress AEP expression (AEP-KD1, AEP-KD2). The effect of AEP on LSECs proliferation, apoptosis and migration were subsequently determined by a CCK8 assay, flow cytometry and wound-healing and Transwell assays, respectively, in AEP knocked-down and control LSECs. The expression of the endothelial cell surface markers CD31, CD34 and von Willebrand factor (vWF) were detected by immunofluorescence assay and western blot. The angiogenic factors, vascular endothelial growth factor receptor 2 (VEGFR2) and interleukin 8 (IL 8) were detected by real-time PCR and western blot. The effect of AEP on vessel tube formation by LSECs was examined by Matrigel(TM) tube-formation assay. Phosphoinositide 3-kinase (PI3K)/Akt expression and phosphorylation were detected by western blot. Results: AEP was effectively knocked down by lentivirus infection in LSECs. Down-regulation of AEP expression significantly decreased proliferation and migration and increased apoptosis of LSECs. Moreover, expression levels of the endothelial cell surface markers CD31, CD34 and vWF, as well as angiogenic factors VEGFR2 and IL 8, were also reduced after AEP was knocked-down. The vessel tube formation abilities of AEP-KD1 and AEP-KD2 LSECs were significantly inhibited compared with LSECs without AEP knocked-down. Down-regulation of AEP also inhibited the phosphorylation of PI3K and Akt. Conclusion: AEP promotes LSECs angiogenesis in vitro, possibly via the PI3K/Akt pathway. AEP may therefore be a potential therapeutic target for preventing the progression of liver fibrosis


No disponible


Assuntos
Humanos , Cisteína Endopeptidases/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Neovascularização Patológica/fisiopatologia , Insuficiência Hepática Crônica Agudizada/fisiopatologia , Capilares/efeitos dos fármacos , Células Endoteliais/efeitos dos fármacos , Antígenos CD34/metabolismo , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Apoptose/fisiologia , Progressão da Doença , Citometria de Fluxo/métodos
9.
Actas dermo-sifiliogr. (Ed. impr.) ; 109(2): 155-161, mar. 2018. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-172388

RESUMO

Introducción y objetivo: Las malformaciones capilares son las malformaciones vasculares más frecuentes en la infancia. El tratamiento de elección sigue siendo el láser de colorante pulsado (LCP), sin embargo, la resolución completa con este habitualmente no se consigue, motivo por el que se siguen buscando otras alternativas terapéuticas. En este estudio comunicamos nuestra experiencia con el láser dual secuencial de LCP y Nd:YAG. Material y métodos: Se efectuó un estudio retrospectivo y descriptivo de los pacientes con malformaciones capilares tratados con el láser dual de LCP y Nd:YAG desde 2006 hasta 2011. Cuatro dermatólogos valoraron el grado de eficacia en una escala del 10 al 0. Se analizó la posibilidad de factores predictores de mejor respuesta al tratamiento: sexo, color de la lesión, existencia de hipertrofia asociada y tamaño de la malformación. Se recogieron igualmente los efectos secundarios. Resultados: Se incluyeron 71 pacientes, presentando el conjunto de ellos una mejoría estadísticamente significativa tras el tratamiento. Las malformaciones de coloración violácea que tenían hipertrofia asociada y las de menor tamaño se asociaron con una mejor respuesta. Se produjeron efectos adversos en un 26,76% de los pacientes, siendo la presencia de zonas atróficas puntuales el más frecuente. Conclusiones: Consideramos que el láser dual de LCP y Nd:YAG es una alternativa eficaz para el tratamiento de malformaciones capilares en paciente seleccionados (AU)


Introduction and objective: Capillary malformations are the most common vascular malformations in childhood. The current treatment of choice is pulsed dye laser (PDL) therapy, but this frequently does not result in complete resolution. The search for alternative treatment strategies thus continues. In this study we describe our experience with the use of sequential dual-wavelength PDL and Nd:YAG laser therapy in patients with capillary malformations. Material and methods: We conducted a retrospective, descriptive study of patients with capillary malformations treated with dual-wavelength PDL and Nd:YAG laser therapy between 2006 and 2011. Four dermatologists rated the effectiveness of treatment on a scale of 10 to 0. We also investigated the potential value of the following factors as predictors of better treatment response: sex, malformation size and color, and presence of associated hypertrophy. Adverse effects were also analyzed. Results: We studied 71 patients and most of them experienced a statistically significant improvement after treatment. More favorable responses were observed for violaceous malformations, lesions with associated hypertrophy, and smaller lesions. Adverse effects were reported for 26.76% of patients, and the most common effect was the appearance of isolated areas of skin atrophy. Conclusions: We consider that sequential dual-wavelength PDL and ND:YAG laser therapy is an effective alternative for treating capillary malformations in selected patients (AU)


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto Jovem , Adulto , Idoso , Malformações Vasculares/diagnóstico , Malformações Vasculares/tratamento farmacológico , Malformações Vasculares/terapia , Capilares/lesões , Malformações Vasculares/complicações , Malformações Vasculares/prevenção & controle , Capilares/patologia , Lasers
10.
Prog. obstet. ginecol. (Ed. impr.) ; 61(1): 63-68, ene.-feb. 2018. ilus
Artigo em Espanhol | IBECS | ID: ibc-171506

RESUMO

El patrón sinusoidal se define como una frecuencia cardíaca fetal que simula una onda sinusoidal suave, con periodicidad de tres a cinco ciclos por minuto y una duración de, al menos, 20 minutos. Es un patrón muy poco frecuente cuyo mecanismo se cree que se produce en respuesta a la hipoxemia fetal moderada, a menudo secundaria a la anemia fetal. La anemia fetal puede ser debida a una hemorragia aguda o a un proceso crónico. Las causas de anemia fetal incluyen hemorragia feto-materna, hemorragia fetal iatrogénica, hemorragia fetal secundaria a rotura de vasa previa o desprendimiento de placenta, la aloinmunización y la infección por parvovirus. Se describen tres casos de anemia neonatal grave, secundarias a transfusión feto-materna, no diagnosticadas durante la gestación, que debutan con un registro con patrón sinusoidal atípico. Lo denominamos atípico, ya que no cumple los criterios estrictos de patrón sinusoidal, y visualmente son registros que podrían pasar inadvertidos, y no ser catalogados como tal, y sin embargo todos los casos se asociaron a cesáreas urgentes por riesgo de pérdida de bienestar fetal y anemias severas en los neonatos (AU)


A sinusoidal heart rate pattern simulates a smooth sine wave, with periodicity of three to five cycles per minute and lasting for at least 20 minutes. It is a very rare pattern whose mechanism is believed to be a response to a moderate fetal hypoxemia, often secondary to fetal anemia. Fetal anemia can be due to acute bleeding or a chronic process; causes include fetomaternal hemorrhage, iatrogenic fetal bleeding, fetal bleeding secondary to vasa previa or placental abruption, alloimmunization, and parvovirus infection. 3 cases of severe neonatal anemia secondary to fetal-maternal transfusion, undiagnosed during pregnancy, debuting with an atypical sinusoidal pattern are described. We call it atypical because it does not meet the strict criteria for sinusoidal pattern, and visually, this kind of fetal heart rate monitoring could pass unnoticed and not be classified as such, but all cases were associated with urgent cesarean for risk fetal and severe anemia in newborns (AU)


Assuntos
Humanos , Feminino , Gravidez , Transfusão Feto-Materna/complicações , Anemia Neonatal/etiologia , Capilares/fisiopatologia , Cardiotocografia/métodos , Doenças Fetais/diagnóstico
11.
Med. clín (Ed. impr.) ; 150(4): 131-137, feb. 2018. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-170608

RESUMO

Introducción y objetivos: Las alteraciones microvasculares se han asociado a la mayoría de los factores de riesgo cardiovascular, así como a múltiples enfermedades inflamatorias. Sin embargo, se desconoce si estas anomalías son más frecuentes en pacientes con enfermedades coronarias. Nuestro objetivo fue evaluar si existe una relación entre la presencia de cardiopatía isquémica y la existencia de alteraciones capilares funcionales y estructurales en la microcirculación cutánea evaluada mediante videocapilaroscopia. Material y métodos: Comparamos 2 muestras emparejadas de 30 participantes con o sin cardiopatía isquémica pero con un perfil antropométrico y clínico similar. Realizamos una videocapilaroscopia en el dorso del tercer dedo de la mano no dominante cuantificando la densidad capilar basal, así como la densidad capilar tras la oclusión arterial y venosa para evaluar su funcionalidad y la densidad capilar máxima. También calculamos el reclutamiento capilar. Resultados: La densidad microvascular fue significativamente menor en los pacientes con cardiopatía isquémica que en los controles tanto a nivel basal como tras el estudio dinámico (oclusión arterial y venosa). No encontramos diferencias en el reclutamiento capilar. Conclusiones: En nuestro estudio, los pacientes con cardiopatía isquémica presentaban cambios microvasculares tanto funcionales como estructurales. Dado que estos resultados han sido obtenidos de una pequeña muestra, se precisarán estudios que valoren la microcirculación en pacientes coronarios y si esta está relacionada con la respuesta terapéutica y/o el pronóstico de la enfermedad (AU)


Introduction and objectives: Microcirculation disturbances have been associated to most of the cardiovascular risk factors as well as to multiple inflammatory diseases. However, whether these abnormalities are specifically augmented in patients with coronary heart disease is still unknown. We aimed to evaluate if there is a relationship between the presence of coronary heart disease and the existence of functional and structural capillary abnormalities evaluated in the cutaneous microcirculation by videocapillaroscopy. Material and methods: Two matched samples of 30 participants with and without coronary heart disease but with similar clinical and anthropometric characteristics were evaluated by videocapillaroscopy at the dorsal skin of the third finger of the non-dominant hand. We calculated basal capillary density as well as capillary density after a period of arterial and venous occlusion in order to evaluate functionality and maximum capillary density. We also measured capillary recruitment. Results: capillary density at rest was significantly lower in patients suffering from coronary heart disease than in controls. This fact was also found after dynamic tests (arterial and venous occlusion), suggesting functional impairments. Capillary recruitment of the samples was not different in our sample. Conclusions: In our study, patients with coronary heart disease exhibit functional and structural microvascular disturbances. Although this is a very preliminary study, these findings open the door for further studying the microvascular functionality in coronary patients and how it relates to the response to treatment and/or the prognosis of the disease (AU)


Assuntos
Humanos , Masculino , Feminino , Isquemia Miocárdica/diagnóstico , Fatores de Risco , Prognóstico , Pele/irrigação sanguínea , Doenças Vasculares/diagnóstico , Angioscopia Microscópica/métodos , Microcirculação , Microvasos/patologia , Antropometria/métodos , Laparoscopia/métodos , Análise de Variância , Capilares/patologia , Índice de Massa Corporal , Modelos Lineares
13.
Cir. pediátr ; 30(3): 162-168, jul. 2017. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-168012

RESUMO

Objetivos. Las anomalías vasculares de presentación neonatal suponen un reto diagnóstico por la ausencia de semiología florida, de historia evolutiva y la conveniencia de restringir pruebas diagnósticas agresivas. El objetivo es revisar las características de los casos neo- natales presentados a nuestra Unidad de Anomalías Vasculares en los últimos 5 años. Material y métodos. Se recogen todos los casos de sospecha de anomalía vascular presentados a nuestra Unidad antes de 1 mes de edad entre 2010 y 2015. Se revisa el momento del diagnóstico en relación con la anomalía, las pruebas diagnósticas y los tratamientos efectuados con su cronología. Se comparan el diagnóstico de presunción y el de certeza, cuando lo hay. Resultados. Se incluyen 26 pacientes: 15 tumores vasculares, 2 de ellos con afectación visceral (6 hemangiomas infantiles (HI), 3 NICH, 4 RICH, 1 hemangioma en penacho, 1 tumor vascular hepático no especificado. 3 malformaciones venosas: 2 con RM equívoca y una malformación venosa hiperqueratótica. 4 malformaciones linfáticas: 3 macroquísticas y una microquística. 2 lesiones muy vasculares que se diagnosticaron posteriormente (fibrosarcoma y adiponecrosis) y no eran anomalías vasculares. Solo 3 pacientes tenían diagnóstico prenatal, las malformaciones linfáticas macroquísticas. Conclusión. El diagnóstico preciso de las anomalías vasculares durante el primer mes de vida es difícil, incluso con RM. En pocos casos se necesita un tratamiento precoz, por lo que conviene dar tiempo a la evolución, al menos durante unas semanas. Los diferentes tipos de tratamiento (observación, propranolol, biopsia, láser, embolización, exéresis) dependerán de la patología a tratar. Una observación continuada puede evitar procedimientos y riesgos innecesarios (AU)


Objective. Vascular anomalies in the neonatal period are a diagnostic challenge for the lack of evident signs, symptoms and follow-up, and the convenience of restricting aggressive diagnostic tests. The aim of this work is to review the characteristics of neonatal cases presented to our Vascular Anomalies Unit in the last 5 years. Materials and methods. All cases of suspected vascular anomaly presented to our unit before 1 month of age between 2010 and 2015 were reviewed, diagnostic tests and treatments carried out with chronology were analyzed. Presumptive diagnosis and final diagnosis (when available) were compared. Results. Fifteen vascular tumors were found, 2 with visceral involvement: 6 infantile hemangiomas (IH), 3 NICH, 4 RICH, 1 tufted hemangioma, 1 unspecified liver vascular tumor, 3 venous malformations (2 equivocal MRI and a hyperkeratotic venous malformation), 4 lymphatic malformations, 3 of them macrocystic, and 2 vascular lesions that were diagnosed of fibrosarcoma and sclerema neonatorum and they were not vascular anomalies. Only 3 patients with macrocystic lymphatic malformations had prenatal diagnosis. Conclusion. Accurate diagnosis of vascular anomalies during the first month of life is difficult, even with MRI. Only in a few cases early treatment is needed, so it is worth taking time to follow-up. Different types of treatment (observation, propranolol, biopsy, laser, embolization, and resection) will depend on the condition to be treated. A continuous observation can avoid unnecessary procedures and risks (AU)


Assuntos
Humanos , Recém-Nascido , Malformações Vasculares/diagnóstico , Hemangioma/diagnóstico , Esclerema Neonatal/diagnóstico , Hemangioma/cirurgia , Capilares/anormalidades , Capilares/cirurgia , Patologia/métodos , Hemangioma/tratamento farmacológico , Propranolol/uso terapêutico , Angiografia/métodos , Hemorragia/diagnóstico , Anormalidades Linfáticas/diagnóstico por imagem
14.
J. physiol. biochem ; 73(1): 1-4, feb. 2017. ilus
Artigo em Inglês | IBECS | ID: ibc-168387

RESUMO

Obesity is a leading health problem facing the modern world; however, no effective therapy for this health issue has yet been developed. A promising research direction to identify novel therapies to prevent obesity has emerged from discoveries on development and function of brown/brite adipocytes in mammals. Importantly, there is evidence for the presence and function of active thermogenic brown adipocytes in both infants and adult humans. Several new investigations have shown that thermogenic adipocytes are beneficial to maintain glucose homeostasis, insulin sensitivity, and a healthy body fat content. Such thermogenic adipocytes have been considered as targets to develop a therapy for preventing obesity. This short review seeks to highlight recent findings on the development and function of brown/brite adipocytes in humans and to discuss potential treatments based on these adipocytes to reduce obesity and its related disorders (AU)


No disponible


Assuntos
Humanos , Animais , Adipócitos Bege/metabolismo , Adipócitos Marrons/metabolismo , Adipogenia , Modelos Biológicos , Termogênese , Adipócitos Brancos , Adiposidade , Capilares/citologia , Resistência à Insulina , Obesidade , Células Estromais/citologia
15.
Arch. bronconeumol. (Ed. impr.) ; 51(5): e23-e24, mayo 2015. tab
Artigo em Espanhol | IBECS | ID: ibc-139083

RESUMO

La microangiopatía trombótica (MAT) es una complicación infrecuente asociada a los anticalcineurínicos en el trasplante pulmonar, independiente de la enfermedad de base de los pacientes trasplantados. Habitualmente se presenta como formas incompletas, lo que dificulta el diagnóstico, que suele ser tardío, provocando irreversibilidad de las lesiones. Es independiente del tiempo de trasplante y en muchos casos existe infección concomitante, lo que tiende a ocultar el diagnóstico. Los casos presentados comparten el agente causal y la presencia de infección concomitante. El tratamiento ha variado en los últimos años, recomendándose la plasmaféresis o, más recientemente, el anticuerpo eculizumab. No obstante, la retirada o cambio del anticalcineurínico causante es la medida más coste-efectiva. La MAT podría tratarse de una entidad infradiagnosticada a tener en cuenta en pacientes trasplantados


Thrombotic microangiopathy (TMA) is a rare complication associated with the use of calcineurin inhibitors in lung transplantation, irrespective of the underlying disease of the graft recipient. It usually occurs in incomplete forms, complicating and delaying diagnosis until damage is already irreversible. It is unrelated to time from transplantation and often presents with concomitant infection, which tends to confound diagnosis. The cases discussed here have a common causative agent and all present with concomitant infection. Treatment recommendations have changed in recent years with the introduction of plasmapheresis or, more recently, the availability of the antibody eculizumab. Notwithstanding, the most cost-effective measure is withdrawal or switching of the calcineurin inhibitor. TMA is an underdiagnosed clinical entity that should be considered in the management of transplantation patients


Assuntos
Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Transplante de Pulmão , Microangiopatias Trombóticas/diagnóstico , Microangiopatias Trombóticas/tratamento farmacológico , Microangiopatias Trombóticas/complicações , Tacrolimo/uso terapêutico , Plasmaferese , Imunossupressão , Insuficiência Renal Crônica/diagnóstico , Diagnóstico Precoce , Resultado do Tratamento , Anemia Hemolítica/diagnóstico , Trombocitopenia/diagnóstico , Capilares/lesões , Artérias/lesões , Doenças do Sistema Nervoso/diagnóstico , Febre/diagnóstico
16.
Arch. Soc. Esp. Oftalmol ; 89(12): 471-476, dic. 2014. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-135434

RESUMO

OBJETIVO: Estudiar los cambios morfológicos y morfométricos producidos en los capilares de la cabeza del nervio óptico (NO) y de su porción inicial, después de la elevación experimental de la presión intraocular (PIO). MATERIAL Y MÉTODOS: Se utilizaron ratas Wistar que fueron sometidas a cauterización de 3 venas epiesclerales, con el resultado inmediato de elevación de la PIO, manteniéndose esta durante 3 meses. Se realizaron secciones sagitales del globo ocular y se aplicaron técnicas inmunohistoquímicas, mediante un anticuerpo para GLUT-1. Se procedió al recuento de los capilares GLUT-1 positivo y se midieron área, perímetro y diámetro medio. RESULTADOS: El examen microscópico de las secciones del NO de las ratas controles demostró una menor densidad de capilares y un mayor calibre de los mismos en la región prelaminar, respecto a las demás regiones del NO (p < 0,05). Cuando se compararon los grupos control y experimental se observó una disminución en la densidad de capilares (excepto en la región prelaminar) y un menor tamaño de los mismos en todas las zonas del NO analizadas, menos evidente en la porción inicial (p < 0,05). CONCLUSIONES: El aumento de la PIO se relaciona con cambios cualitativos y cuantitativos de los capilares de las regiones laminar y poslaminar de la cabeza del NO, y parecen recuperarse hacia parámetros compatibles con la normalidad en la porción inicial del NO, donde el colapso vascular es menos evidente. Estos hallazgos podrían explicar la reducción significativa del flujo sanguíneo ocular observada en pacientes con glaucoma primario de ángulo abierto


AIM: To study the morphological and morphometric changes produced in the capillaries of the optic nerve (ON) head and initial portion after the experimental increase in intraocular pressure (IOP). MATERIAL AND METHODS: Wistar rats underwent cauterization of three episcleral veins, which produced an immediate increase in the IOP, and was maintained for 3 months. Sagittal sections of the eyeball were studied with immunohistochemical techniques, using a primary antibody to GLUT-1. The GLUT-1 positive capillaries were counted, and measurements were made of the area, perimeter and mean diameter. RESULTS: Microscopic examination of sections of the ON of control rats revealed a lower density and larger caliber of capillaries in the prelaminar region as compared with the other regions of the ON (P<.05). Comparison between the control and the experimental groups showed a reduction in capillary density (except in the prelaminar region) and a smaller size in all the areas of the ON studied, but less evident in the initial portion (P<.05). CONCLUSIONS: The increase in IOP was associated with significant qualitative and quantitative changes in the capillaries of the laminar and poslaminar regions of the ON head. These changes appear to return towards parameters compatible with normality in the initial portion of the ON, an area where the vascular collapse was less evident. These findings might explain the significant reduction in ocular blood flow seen in patients with primary open-angle glaucoma


Assuntos
Animais , Ratos , Capilares/ultraestrutura , Nervo Óptico/irrigação sanguínea , Glaucoma/patologia , Biomarcadores , Hipóxia Celular , Modelos Animais de Doenças , Transportador de Glucose Tipo 1/análise , Pressão Intraocular , Microglia/fisiologia , Disco Óptico/irrigação sanguínea , Ratos Wistar
18.
An. pediatr. (2003, Ed. impr.) ; 79(6): 374-376, dic. 2013. ilus
Artigo em Espanhol | IBECS | ID: ibc-117012

RESUMO

El síndrome malformación capilar-malformación arteriovenosa es una rasopatía de herencia autosómico-dominante, que se caracteriza por la presencia de malformaciones capilares cutáneas multifocales que se asocian a malformaciones vasculares y fístulas arteriovenosas en otras localizaciones de la economía. Las rasopatías incluyen un conjunto de síndromes que presentan en común mutaciones en las proteínas de la vía RAS/MAPK. Comentamos las implicaciones clínicas y las pruebas necesarias para su diagnóstico a raíz de un caso recientemente diagnosticado en nuestra unidad (AU)


Capillary malformation-arteriovenous malformation syndrome is an autosomal dominant condition in which multifocal capillary malformations and arteriovenous fistulas form indifferent organs. RASopathies include a set of common syndromes that have mutations on the Ras/MAPK pathway. We discuss the clinical implications and tests necessary for its diagnosis (AU)


Assuntos
Humanos , Feminino , Pré-Escolar , Malformações Arteriovenosas/diagnóstico , Capilares/anormalidades , Malformações Vasculares/diagnóstico , Proteínas ras , Mitógenos , Fístula Arteriovenosa/genética
19.
Actas dermo-sifiliogr. (Ed. impr.) ; 104(8): 710-713, oct. 2013. ilus
Artigo em Espanhol | IBECS | ID: ibc-116345

RESUMO

El síndrome malformaciones capilares-malformaciones arteriovenosas es un tipo de malformación vascular poco frecuente que se describió en 2003. Se hereda de forma autosómica dominante, y se ha objetivado que está causado por mutaciones heterocigotas en el gen RASA1, que codifica la proteína RASp21. Dicho síndrome se caracteriza por malformaciones capilares pequeñas y múltiples que se asocian con malformaciones arteriovenosas o fístulas arteriovenosas, tanto en los individuos afectos como en sus familias. Describimos aquí 2 nuevos casos familiares de este síndrome que hemos diagnosticado y estudiado en nuestro centro, tanto desde el punto de vista clínico como genético (AU)


Capillary malformation-arteriovenous malformation syndrome is a rare type of vascular malformation first described in 2003. It is an autosomal dominant inherited disorder that has been reported in association with heterozygous mutations in the RASA1 gene, which encodes the protein RASp21. The clinical picture is characterized by multiple small capillary malformations which are associated with either arteriovenous malformations or arteriovenous fistulas in both the affected individual and other members of their family. We describe 2 new familial cases of this syndrome that were clinically and genetically diagnosed and studied in our hospital (AU)


Assuntos
Humanos , Capilares/anormalidades , Doenças do Cabelo/complicações , Malformações Arteriovenosas/complicações , Fístula Arteriovenosa/epidemiologia , Aberrações Cromossômicas
20.
Actas dermo-sifiliogr. (Ed. impr.) ; 103(8): 661-678, oct. 2012. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-108510

RESUMO

Las malformaciones vasculares (MV) son errores innatos en el desarrollo embriológico de los vasos sanguíneos que están presentes siempre desde el nacimiento. Por ello deben diagnosticarse en la infancia y, en los casos en que sea necesario, tratarlas para evitar complicaciones posteriores. La tendencia actual es a clasificar estas lesiones en cuanto al flujo que presentan y en cuanto al tipo de vaso predominante en las mismas. Dada la complejidad que presentan estas lesiones, y en muchos casos su poca frecuencia, deben abordarse desde un punto de vista multidisciplinario en centros de anomalías vasculares. Además, la asociación de malformaciones vasculares con cuadros sindrómicos está cada vez mejor definida, lo que hace necesario su conocimiento para un mejor abordaje de estos enfermos (AU)


Vascular malformations are inborn errors of vascular embryogenesis present at birth that should be diagnosed in childhood and, when necessary, treated to prevent later complications. The current trend is to classify these lesions according to flow characteristics and the predominant type of vascular channel affected. Given the complexity, and in many cases, the rarity, of vascular malformations, they should be managed by multidisciplinary teams at vascular anomalies centers. Furthermore, because the association between vascular malformations and certain syndromes is becoming increasingly recognized, a better understanding of these lesions will help to improve overall patient management in this setting (AU)


Assuntos
Humanos , Masculino , Feminino , Gravidez , Malformações Vasculares/diagnóstico , Malformações Vasculares/embriologia , Mancha Vinho do Porto/epidemiologia , Malformações Vasculares/fisiopatologia , Malformações Vasculares/classificação , Capilares/patologia , Doenças do Cabelo , Nevo/complicações , Esclerose Tuberosa/complicações
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