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1.
Metas enferm ; 22(6): 66-72, jul. 2019. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-184048

RESUMO

Se presenta un caso clínico de un paciente con elefantiasis y úlceras sobreinfectadas en ambas extremidades inferiores, incluyendo una valoración enfermera, diagnósticos prioritarios, plan de cuidados, justificación del tratamiento elegido y la evolución posterior. La evidencia de infección de las úlceras planteó la necesidad de instaurar una terapia con cadexómero yodado para tratar localmente la infección, e hidrogel para evitar la formación de biopelículas. Transcurrido un mes del alta del paciente se realizó un seguimiento en el que se observó una evolución favorable con aumento de tejido de granulación y tejido epitelial en varias de las lesiones presentes, por lo que se valoró de forma favorable el procedimiento de cura en ambiente húmedo; la mejora en los hábitos higiénico-dietéticos se completó por parte del paciente


We present the clinical case of a patient with elephantiasis and superinfected ulcers in both lower limbs, including the Nursing assessment, primary diagnosis, plan of care, justification for the treatment selected, and subsequent evolution. The evidence of ulcer infection required to initiate therapy with cadexomer iodine in order to treat the infection locally, and hydrogel to prevent the formation of biofilms. Follow-up was conducted one month after the patient was discharged, and favourable evolution was observed, with an increase in granulation tissue and epithelial tissue in many of the lesions; therefore, the procedure of healing in a moist environment was assessed as favourable, and the improvement in hygienic-dietary habits was completed by the patient


Assuntos
Humanos , Masculino , Idoso , Infecção dos Ferimentos/complicações , Infecção dos Ferimentos/enfermagem , Ferimentos e Lesões/enfermagem , Elefantíase/complicações , Cuidados de Enfermagem , Linfedema/enfermagem , Cicatrização , Tecido de Granulação/lesões , Epitélio/lesões , Avaliação em Enfermagem
3.
Arch. Soc. Esp. Oftalmol ; 94(2): 85-89, feb. 2019. ilus
Artigo em Espanhol | IBECS | ID: ibc-180370

RESUMO

Paciente de 66 años en seguimiento por retinopatía diabética refractaria a múltiples modalidades de tratamiento a pesar del buen control metabólico que refiere pérdida de peso progresiva. Por este motivo se decide realizar un estudio sistémico, detectándose anemia, elevación de la velocidad de sedimentación globular e hiperproteinemia a expensas de un pico monoclonal de IgM. Posteriormente, mediante la biopsia de médula ósea y el estudio genético, se llega al diagnóstico de macroglobulinemia de Waldenström. La macroglobulinemia de Waldenström es una patología linfoproliferativa de escasa frecuencia cuya principal manifestación es a través del síndrome de hiperviscosidad. Este puede producir signos oftalmológicos detectables mediante funduscopia y pruebas de imagen. El estudio multimodal es útil en el diagnóstico y seguimiento de la afectación retiniana. La incorporación de la angiografía por tomografía de coherencia óptica permite un estudio más preciso de los trastornos microvasculares que se pueden presentar a nivel del polo posterior


A 66 year-old patient, monitored for diabetic retinopathy refractory to multiple treatment methods despite a good metabolic control, referred to progressive weight loss. For this reason, a systemic study was performed, detecting anaemia, elevation of the erythrocyte sedimentation rate, and hyperproteinaemia due to elevated serum levels of monoclonal IgM. Subsequently, by performing a bone marrow biopsy and genetic study, the diagnosis of Waldenström macroglobulinaemia was made. Waldenström's macroglobulinaemia is a low frequency lymphoproliferative disease, for which the main manifestation is a hyperviscosity syndrome that can produce ophthalmological signs detectable by funduscopy and imaging tests. A multimodal study is useful in the diagnosis and monitoring of retinal involvement. The incorporation of angiography by optical coherence tomography allows a more precise study of the microvascular disorders that may occur at the posterior pole level


Assuntos
Idoso , Retinopatia Diabética/complicações , Retinopatia Diabética/diagnóstico , Retinopatia Diabética/patologia , Macroglobulinemia de Waldenstrom/classificação , Macroglobulinemia de Waldenstrom/diagnóstico , Pacientes/classificação , Doenças Hematológicas/sangue , Doenças Hematológicas/classificação , Doenças Hematológicas/diagnóstico , Epitélio/diagnóstico por imagem , Epitélio/patologia
4.
Arch. Soc. Esp. Oftalmol ; 94(2): 95-99, feb. 2019. ilus
Artigo em Espanhol | IBECS | ID: ibc-180372

RESUMO

La glomerulonefritis membranoproliferativa mediada por complemento es una enfermedad rara, pero puede asociarse a alteraciones retinianas. Por ello, el propósito de este estudio fue valorar una serie de casos con dicho diagnóstico en seguimiento por nefrología en nuestro centro. Se realizó un estudio transversal de 8 pacientes diagnosticados de glomerulonefritis membranoproliferativa mediada por complemento. Se realizó funduscopia, tomografía de coherencia óptica (OCT) y angio-OCT de dominio Swept Source. Uno de los 8 pacientes presentaba depósitos drusenoides que se localizaron bajo el epitelio pigmentario retiniano en la OCT, descartándose la presencia de neovascularización coroidea asociada en la angio-OCT. Por tanto, la glomerulonefritis membranoproliferativa puede producir alteraciones retinianas con drusas o desprendimientos del epitelio pigmentario retiniano, por lo que debe hacerse un adecuado diagnóstico diferencial con la degeneración macular asociada a la edad. Es crucial realizar un seguimiento en estos pacientes ante las posibles complicaciones que pueden desembocar en una pérdida de visión


Complement-mediated membranoproliferative glomerulonephritis is a rare progressive glomerular disease. In some patients it can be associated with retinal lesions. Therefore, the purpose of this study was to assess a case series with this diagnosis in our hospital. A cross-sectional study was conducted on 8 patients diagnosed with complement-mediated membranoproliferative glomerulonephritis. Funduscopy, optical coherence tomography (OCT) and Swept Source domain OCT angiography were performed. Only 1 of the 8 patients showed drusen-like deposits that were located under the retinal pigment epithelium in the OCT, with the presence of associated choroidal neovascularization being ruled out in OCT angiography. Therefore, membranoproliferative glomerulonephritis may produce retinal alterations with drusen or retinal pigment epithelium detachment, and requires an appropriate differential diagnosis to be made with age-related macular degeneration. The follow-up of these patients is important in order to detect vision-threatening complications


Assuntos
Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Glomerulonefrite Membranoproliferativa , Retina/patologia , Pacientes/classificação , Tomografia/classificação , Epitélio/patologia , Drusas Retinianas/classificação , Degeneração Macular/classificação , Degeneração Macular/diagnóstico
5.
Cir. plást. ibero-latinoam ; 44(4): 379-387, oct.-dic. 2018. ilus, graf, tab
Artigo em Espanhol | IBECS | ID: ibc-180084

RESUMO

Introducción y Objetivo: Los estímulos fisiológicos, como la pérdida masiva de peso, proporcionan adaptaciones celulares en las que se alcanza un nuevo equilibrio que preserva la viabilidad de la célula, lo que se conoce como cambios adaptativos celulares. En nuestro trabajo nos planteamos la siguiente hipótesis: ¿podemos demostrar los cambios adaptativos fisiomorfológicos, histológicos e inmunohistoquímicos celulares sufridos por la piel y la grasa de los pacientes con pérdida masiva de peso? Material y Método: Llevamos a cabo un estudio experimental, descriptivo, prospectivo y comparativo de casos y controles, con una muestra al azar dividida en 2 grupos: A, pacientes postbariátricos y B, pacientes no postbariátricos. Resultados: Las muestras de piel de los pacientes postbariátricos presentaron cambios en el epitelio, colágeno, dermis y adipocitos. Conclusiones: Demostramos la presencia de cambios adaptativos celulares en la piel y la grasa de los pacientes con pérdida masiva de peso, que nos permiten establecer que existe un patrón histológico postbariátrico. Estos datos mejoran nuestro enfoque médico y quirúrgico en la búsqueda de menores complicaciones con mejores resultados en las cirugías de readaptación de contorno corporal a las que pueden someterse dichos pacientes


Background and Objective: The physiological stimuli, such as massive weight loss, provide cellular adaptations in which a new balance that preserves cell viability is reached, what is known as cellular adaptive changes. Our hypothesis in this study was: can we show fisiomorfológic cellular, histological and immunohistochemical adaptive changes suffered by the skin and fat of patients with massive weight loss? Methods: An experimental, descriptive, prospective and comparative case-control study was conducted on a random sample divided into 2 groups: A, postbariátric patients and B, no postbariatric patients. Results: Skin samples from postbariátric patients showed changes in the epithelium, collagen, dermis and fat cells. Conclusions: Cellular adaptive changes in the skin and fat of patients with massive weight loss are shown. These data improve our medical and surgical approach in finding minor complications with better aesthetic results when these patients undergo a body contouring surgery


Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Obesidade/patologia , Obesidade/cirurgia , Cirurgia Bariátrica , Epitélio/patologia , Colágeno/análise , Derme/patologia , Adipócitos/patologia , Estudos Prospectivos , Estudos de Casos e Controles , Imuno-Histoquímica
6.
Prog. obstet. ginecol. (Ed. impr.) ; 61(2): 173-175, mar.-abr. 2018. ilus
Artigo em Espanhol | IBECS | ID: ibc-173670

RESUMO

Introducción: se presenta un caso de endosalpingiosis apendicular diagnosticado casualmente en pieza de apendicectomía. Caso clínico: paciente de 40 años con antecedentes de esterilidad primaria que acudió a urgencias por apendicitis. Se realizó apendicectomía laparoscópica sin incidencias. El estudio anatomopatológico reveló glándulas de epitelio cilíndrico ciliado sugestivas de endosalpingiosis, derivándose a nuestro servicio para valoración ginecológica, que resultó normal. Discusión: la endosalpingiosis es una entidad benigna caracterizada por presencia de epitelio y glándulas de las trompas de Falopio en una localización externa. La endosalpingiosis apendicular es extremadamente rara, con pocos casos descritos, y que constituye un diagnóstico casual por ser asintomática. Sin embargo, en ocasiones puede asociarse a dolor pélvico crónico, infertilidad o trastornos menstruales, por lo que esta patología no debe pasar desapercibida entre los profesionales médicos


Introduction: We report a case of appendiceal endosalpingiosis diagnosed by a routine histopathological examination of an appendicectomy specimen. Case report: A 40-year-old female, with a history of primary infertility, presented with acute appendicitis. A laparoscopic appendicectomy was performed with no complications associated. The pathological study revealed ciliated columnar epithelium suggestive of endosalpingiosis. She was referred to our department for gynecologic examination that was normal. Discussion: Endosalpingiosis is a benign entity defined by the presence of glands lined by tubal-type epithelium with cilliated and secretory cells in sites other than Fallopian tubes. Endosalpingiosis of the appendix is extremely rare and few cases have been reported. This disease is predominantly asymptomatic, however, it can be associated with chronic pelvic pain, infertility or menstrual irregularities


Assuntos
Humanos , Feminino , Adulto , Apendicectomia/métodos , Endometriose/diagnóstico , Infertilidade/complicações , Doenças das Tubas Uterinas/patologia , Endometriose/patologia , Dor Pélvica/etiologia , Dor Abdominal/etiologia , Vagina/diagnóstico por imagem , Vagina/patologia , Doenças das Tubas Uterinas/diagnóstico , Epitélio/diagnóstico por imagem , Epitélio/patologia , Salpingite/diagnóstico por imagem
7.
Rev. esp. patol. torac ; 29(4): 216-225, dic. 2017. graf, ilus
Artigo em Espanhol | IBECS | ID: ibc-170398

RESUMO

En este trabajo usamos dióxido de titanio (TiO2), fabricado mediante nanotecnología. Para demostrar su superioridad respecto al talco, realizamos un estudio in vitro comparando la respuesta pro-inflamatoria de ambos agentes sobre células malignas y mesoteliales benignas; investigando la posible inducción de apoptosis y la posible inhibición de angiogénesis también por ambos agentes. Realizamos cultivo de líneas celulares derivadas de mesotelio humano, procedente de mesotelioma bifásico humano y adenocarcinoma bronquial humano. Las células se co-cultivaron con diferentes dosis de talco y de nanopartículas de TiO2. En todas las muestras de sobrenadantes de los cultivos se analizaron los niveles de diferentes mediadores inflamatorios. La tasa de apoptosis se analizó por la expresión de Caspasa-3. Para el estudio de angiostasis se determinaron los niveles de endostatina mediante técnica ELISA. Observamos que la viabilidad de las células mesoteliales benignas es mucho menor al emplear TiO2. En el caso de las células mesoteliales malignas, se observó el mismo efecto con dosis alta de TiO2. En el adenocarcinoma de pulmón, la viabilidad de estas células expuestas al talco fue netamente inferior a la que se observó en la línea celular benigna. La producción de IL-8 fue mucho mayor por parte de las células mesoteliales neoplásicas que por las benignas y aumentó siguiendo un patrón dosis dependiente frente al talco, mientras que cayó con el TiO2. Según estos resultados, se demuestra que el talco es superior al TiO2 en su capacidad de producir mediadores que favorecerían la pleurodesis para el control del derrame pleural maligno


For this study, we used titanium dioxide (TiO2), produced using nanotechnology. To show its superiority with respect to talc, we completed an in vitro study comparing the pro-inflammatory response of both agents towards malignant and benign mesothelial cells; researching the possible apoptosis induction and possible inhibition of angiogenesis for both agents. We took a culture of cell lines derived from human mesothelioma, originating from human biphasic mesothelioma and human bronchial adenocarcinoma. The cells were cocultured with different doses of talc and TiO2 nanoparticles. The levels of different inflammatory mediators were analyzed for each culture supernatant sample. The apoptosis rate was analyzed using caspase-3 expression. The endostatin levels were determined for the angiostasis study using the ELISA technique. We observed that the viability of the benign mesothelial cells is much lower after using TiO2. In the case of malignant mesothelial cells, the same effect was observed with a high dose of TiO2. In adenocarcinoma of the lung, the viability of these cells exposed to talc was distinctly lower than that which was observed in the benign cell line. IL-8 production was much higher in neoplastic mesothelial cells than in benign cells and increased following a dose-dependent pattern with talc, while it decreased with TiO2. According to these results, we can see that talc is superior to TiO2 in its ability to produce mediators which favor pleurodesis for the control of malignant pleural effusions


Assuntos
Humanos , Titânio/uso terapêutico , Nanotecnologia/métodos , Talco/uso terapêutico , Derrame Pleural/prevenção & controle , Indutores da Angiogênese/uso terapêutico , Nanopartículas/análise , Células Epiteliais , Técnicas In Vitro/métodos , Apoptose , Endostatinas/análise , Derrame Pleural/terapia , Ensaio de Imunoadsorção Enzimática/métodos , Pleurodese/métodos , Sobrevivência Celular , Epitélio
9.
Med. oral patol. oral cir. bucal (Internet) ; 22(3): e314-e323, 1 mayo 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-163198

RESUMO

Background: Primordial Odontogenic Tumor (POT) is a recently described odontogenic tumor characterized by a variably cellular loose fibrous tissue with areas similar to the dental papilla, covered by cuboidal to columnar epithelium that resembles the internal epithelium of the enamel organ, surrounded at least partly by a delicate fibrous capsule. The purpose of this study was to investigate the possible histogenesis and biological behavior of this rare tumor by means of a wide immunohistochemical analysis of its epithelial and mesenchymal components. Material and Methods: The immunoexpression of twenty-three different antibodies were evaluated in four cases of POT. Results: The epithelial cells that cover the periphery of the tumor showed immunopositivity for Cytokeratins 14 and 19, while Amelogenin, Glut-1, MOC-31, Caveolin-1. Galectin-3, PITX2, p53, Bax, Bcl-2, Survivin and PTEN were variably expressed in focal areas. The mesenchymal component of the tumor was positive for Vimentin, Syndecan-1, PITX2, Endoglin (CD105), CD 34, Cyclin D1, Bax, Bcl-2, Survivin and p53. PTEN and CD 90 showed a moderate positivity. BRAF V600E and Calretinin were negative in all samples. Cell proliferation markers (Ki-67, MCM-7) were expressed in < 5% of the tumor cells. Conclusions: According to these immunohistochemical findings, we may conclude that POT is a benign odontogenic tumor in which there is both epithelial and mesenchymal activity during its histogenesis, as there is expression of certain components in particular zones in both tissues that suggests this tumor develops during the immature (primordial) stage of tooth development, leading to its inclusion within the group of benign mixed epithelial and mesenchymal odontogenic tumours in the current World Health Organization classification of these lesions (AU)


No disponible


Assuntos
Humanos , Tumores Odontogênicos/patologia , Imuno-Histoquímica/métodos , Neoplasias Maxilares/patologia , Mesoderma/patologia , Células-Tronco/patologia , Epitélio/patologia
11.
J. physiol. biochem ; 73(1): 133-139, feb. 2017. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-168400

RESUMO

Na+/H+ exchanger isoform 3 (NHE3) dysfunction is thought to contribute to the altered gallbladder absorption that occurs in cholesterol gallstone disease, but the mechanism is unknown. The current study was undertaken to examine the expression, phosphorylation, and subcellular localization of NHE3 in gallbladder epithelium cells (GBECs) of male C57BL/6 mice on a control or lithogenic diet. Thirty-six 8-week-old male C57BL/6 mice were randomly assigned to receive a high cholesterol diet or a regular diet for 8 weeks. Gallstone formation was recorded. Gallbladder bile cholesterol, phospholipid, and total bile acids were examined. RT-PCR was used to measure NHE3 mRNA expression. NHE3 protein expression and subcellular localization were examined by Western blotting and immunofluorescence microscopy, respectively. Gallstones were formed in all mice fed the lithogenic diet. Despite higher NHE3 mRNA expression in gallbladders of the mice on the lithogenic diet than in those on the control diet, there was no significant difference in expression of total NHE3 protein. However, a higher level of NHE3 phosphorylated at serine-552 (P-NHE3) was seen on the lithogenic diet. In immunofluorescence studies, NHE3 protein was expressed both on the apical membrane and in the cytoplasm of mouse GBEC. This pattern of subcellular distribution of NHE3 strongly corroborates an exchanger trafficking mechanism in NHE3 activity regulation in mouse GBEC. We conclude that increased phosphorylation of NHE3 following gallstone formation leads to turnover of the exchanger, resulting in decreased gallbladder concentrating function (AU)


No disponible


Assuntos
Animais , Masculino , Camundongos , Absorção Fisiológica , Epitélio/metabolismo , /metabolismo , Vesícula Biliar/metabolismo , Cálculos Biliares/metabolismo , Regulação da Expressão Gênica , Processamento de Proteína Pós-Traducional , Dieta Hiperlipídica/efeitos adversos , Estabilidade Proteica , Transporte Proteico , Regulação para Cima , RNA Mensageiro/metabolismo , Distribuição Aleatória , Fosforilação , Serina/metabolismo
12.
Arch. Soc. Esp. Oftalmol ; 91(12): 599-603, dic. 2016. ilus
Artigo em Espanhol | IBECS | ID: ibc-158448

RESUMO

CASOS CLÍNICOS: Se presentan 2 casos clínicos de epiteliopatía difusa crónica (EDC) con respuesta favorable a espironolactona. En el primer paciente se visualizan regiones de desprendimiento neurosensorial (DNS), y en el segundo múltiples DNS, quistes intrarretinianos secundarios y desprendimiento del epitelio pigmentario de la retina. DISCUSIÓN: Tras el tratamiento con espironolactona, se observa mejoría de la agudeza visual y de las alteraciones estructurales en ambos pacientes (disminución del DNS y quistes intrarretinianos). Los fármacos antagonistas de los receptores de aldosterona usados para formas persistentes de coriorretinopatía central serosa (CRCS) tienen resultados alentadores, y podrían ser una alternativa terapéutica en la EDC


CASE REPORT: Two cases are presented of patients with chronic diffuse epitheliopathy (CDE) that showed a favourable response when treated with spironolactone. The first patient had regions of neurosensory detachment (DNS) and the second, multiple DNS, secondary intra-retinal cysts and retinal pigment epithelium detachment. DISCUSSION: After treatment with spironolactone, both patients showed a visual acuity improvement and structural changes (reduced neurosensory retinal detachment and cystoid degeneration). Aldosterone receptor agonists (ARA) used for persistent forms of chronic central serous chorioretinopathy show encouraging results and could represent a therapeutic alternative for CDE


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Espironolactona/uso terapêutico , Epitélio/cirurgia , Epitélio , Retina/cirurgia , Retina , Aldosterona/uso terapêutico , Acuidade Visual/fisiologia , Coriorretinopatia Serosa Central/complicações , Coriorretinopatia Serosa Central/cirurgia , Retina/lesões
13.
Rev. patol. respir ; 19(4): 132-135, oct.-dic. 2016. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-159485

RESUMO

El síndrome de hemorragia alveolar difusa (SHAD) tiene su origen en enfermedades muy diversas. La característica común es la presencia de sangre en el espacio alveolar como consecuencia de una lesión de la membrana basal del endotelio y del epitelio alveolar. Numerosas etiologías pueden dar origen a este síndrome y se clasifican según la presencia o no de capilaritis pulmonar que es el hallazgo más frecuente. Presentamos el caso de una mujer de 77 años que mostraba infiltrados alveolares difusos en la radiografía de tórax que fueron compatibles con hemorragia alveolar difusa por los hallazgos del lavado broncoalveolar y que el diagnóstico final fue de vasculitis p-ANCA positivo


Diffuse alveolar hemorrhage has its origin in various types of disease, all of them characterized by the widespread extravasation of red blood cells into the pulmonary alveolar spaces. It is associated with a disruption of the alveolar and capillary basement membranes. Multiple etiologies can origin this syndrome. They are divided into pulmonary capillaritis-and non capillaritis. Pulmonary capillaritis represents the most common finding. We present the case of a 77 year-old with diffuse alveolar infiltrates in chest imaging and alveolar hemorrhage that was later diagnosed as antineutrophil cytoplasmic antibodies-associated vasculitis


Assuntos
Humanos , Feminino , Idoso , Endotélio/fisiologia , Epitélio/patologia , Radiografia Torácica/métodos , Lavagem Broncoalveolar/métodos , Lavagem Broncoalveolar/normas , Vasculite/sangue , Serviços Médicos de Emergência/métodos , Hipercapnia/sangue , Pneumologia/normas , Epitélio/metabolismo , Radiografia Torácica/instrumentação , Lavagem Broncoalveolar/classificação , Lavagem Broncoalveolar , Vasculite/patologia , Serviços Médicos de Emergência/normas , Hipercapnia/complicações , Pneumologia/classificação
14.
Cir. plást. ibero-latinoam ; 42(2): 141-147, abr.-jun. 2016. ilus, graf, tab
Artigo em Espanhol | IBECS | ID: ibc-154967

RESUMO

Introducción y Objetivos. En la actualidad existen una gran cantidad de apósitos y coberturas para el tratamiento de las heridas de espesor parcial; la elección depende de la experiencia del cirujano y sobre todo de la disponibilidad y recursos existentes. Las estatinas (inhibidores de la Hidroxi-Metil-CoA reductasa) se usan en el tratamiento de la hipercolesterolemia, aunque recientemente se han encontrado propiedades antinflamatorias, estimulantes de la angiogénesis y linfangiogénesis, antifibróticas, y especialmente estimuladoras de los queratinocitos. Nos proponemos comparar la epitelización e inflamación de las heridas en un modelo animal con la aplicación de simvastatina tópica, inyectable y apósito de hidrocoloide. Material y Método. Empleamos 20 ratas Wistar en 4 grupos, cada uno con 5 ratas, a las que practicamos heridas de espesor parcial por dermoabrasión en el dorso. A cada grupo se le aplicó gasa de organza, apósito de hidrocoloide, simvastatina tópica y simvastatina inyectada, respectivamente. Descubrimos las heridas a los 7 días y sacrificamos a los animales. Tomamos biopsias de las heridas y comparamos el grado de epitelización de cada grupo. Resultados. Al comparar la simvastatina en preparación tópica con el grupo de gasa de organza y el grupo de apósito de hidrocoloide, encontramos un mayor porcentaje de epitelización en el grupo tratado con simvastatina tópica, con una diferencia estadísticamente significativa (p<0.05). Las heridas tratadas con simvastatina tópica demostraron menor inflamación, menor fibrosis, mayor epitelización (queratinocitos), mayor cantidad de fibroblastos y no presentaron costra, de forma estadísticamente significativa. Conclusiones. La cobertura de heridas de espesor parcial con simvastatina tópica es más efectiva en epitelización y efecto antinflamatorio, en comparación con la simvastatina inyectable, los apósitos de hidrocoloide y las gasas de organza (AU)


Background and Objective. There are various alternatives and dressings for split thickness skin wounds, the choice depends on the experience and training of each surgeon. Statins, which are normally used for the treatment of hypercholesterolemia, have proven to induce and antinflammatory effect on wound keratinocytes, which provokes a faster re-epithelialization on in vitro studies. Our aim was to compare epithelialization and anti-inflammatory properties in split thickness wounds of rats, using hydrocolloid dressings versus a simvastatin based emulsion or simvastatin injected. Methods. We used 20 Wistar rats divided in 4 groups in which split thickness wounds were induced in the dorsum by dermoabrasion. Each group was treated with organza gauze, hydrolloid dressings, simvastatin cream and injectable simvastatin in the wound, respectively. Seven days later, the wounds were exposed and the animals where sacrificed. The wounds were analyzed with digital photographs and biopsies where taken in order to compare the epithelization percentage in each group. Results. There was a statistically significant improvement (p<0.05) in wound rate epithelialization in the rats treated with topic simvastatin. The wounds treated with topic simvastatin had less inflammation, less fibrosis, no evidence of crusting and a higher number of fibroblast. Conclusions. Split thickness skin wounds coverage with topical simvastatin is most effective in epithelialization and antinflamatory effect compared with injectable simvastatin, organza gauze dressings and hydrocolloid dressings (AU)


Assuntos
Animais , Ratos , Sinvastatina/farmacocinética , Curativos Hidrocoloides , Cicatrização , Técnicas de Fechamento de Ferimentos , Modelos Animais de Doenças , Epitélio , Anti-Inflamatórios/farmacocinética
15.
Rev. esp. med. nucl. imagen mol. (Ed. impr.) ; 35(2): 88-95, mar.-abr. 2016. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-148914

RESUMO

Objective. Metabolic tumour volume (MTV) and total lesion glycolysis (TLG) from 18F-FDG PET/CT are emerging prognostic biomarkers in various solid neoplasms. These volumetric parameters and the SUVmax have shown to be useful criteria for disease prognostication in preoperative and post-treatment epithelial ovarian cancer (EOC) patients. The purpose of this study was to evaluate the utility of 18F-FDG PET/CT measurements to predict survival in patients with recurrent EOC. Material and methods. Twenty-six patients with EOC who underwent a total of 31 18F-FDG PET/CT studies for suspected recurrence were retrospectively included. SUVmax and volumetric parameters whole-body MTV (wbMTV) and whole-body TLG (wbTLG) with a threshold of 40% and 50% of the SUVmax were obtained. Correlation between PET parameters and progression-free survival (PFS) and the survival analysis of prognostic factors were calculated. Results. Serous cancer was the most common histological subtype (76.9%). The median PFS was 12.5 months (range 10.7-20.6 months). Volumetric parameters showed moderate inverse correlation with PFS but there was no significant correlation in the case of SUVmax. The correlation was stronger for first recurrences. By Kaplan-Meier analysis and log-rank test, wbMTV 40%, wbMTV 50% and wbTLG 50% correlated with PFS. However, SUVmax and wbTLG 40% were not statistically significant predictors for PFS. Conclusion. Volumetric parameters wbMTV and wbTLG 50% measured by 18F-FDG PET/CT appear to be useful prognostic predictors of outcome and may provide valuable information to individualize treatment strategies in patients with recurrent EOC (AU)


Objetivo. El metabolic tumour volume (MTV) y la total lesion glycolysis (TLG) obtenidos de la 18F-FDG PET/TC son biomarcadores pronósticos emergentes en varias neoplasias. Estos parámetros volumétricos junto con el SUVmax son útiles para determinar el pronóstico preoperatorio y post-tratamiento de las pacientes con cáncer epitelial de ovario (CEO). El objetivo fue determinar la utilidad de los parámetros de la 18F-FDG PET/TC para predecir la supervivenvia de las pacientes con CEO recurrente. Material y métodos. Se incluyeron retrospectivamente 26 pacientes con CEO a las que se les realizó 31 estudios 18F-FDG PET/TC por sospecha de recidiva. Se obtuvo el valor del SUVmax, whole-body MTV (wbMTV) y whole-body TLG (wbTLG) con un umbral del 40% y 50% del SUVmax. Se estimó la correlación entre los parámetros PET y el intervalo libre de progresión (ILP) y se realizó análisis de supervivencia segun factores pronósticos. Resultados. El subtipo histológico más frecuente fue cáncer seroso (76,9%). La mediana del ILP fue 12,5 meses (rango 10,7-20,6 meses). Se observó correlación inversa moderada entre los parámetros volumétricos e ILP, sin evidenciar correlación significativa con SUVmax. La correlación fue más fuerte para primeras recidivas. Las curvas de supervivencia mostraron correlación entre wbMTV 40%, wbMTV 50% y wbTLG 50% con ILP. SUVmax y wbTLG 40% no fueron predictores de ILP de forma estadísticamente significativa. Conclusión. Los parámetros wbMTV y wbTLG 50% de la 18F-FDG PET/TC podrían utilizarse como factores pronósticos ofreciendo una valiosa información para individualizar el tratamiento de las pacientes con CEO recurrente (AU)


Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Neoplasias Ovarianas , Prognóstico , Fluordesoxiglucose F18 , Tomografia por Emissão de Pósitrons/métodos , Tomografia por Emissão de Pósitrons , Ovário/anatomia & histologia , Ovário/patologia , Ovário , Epitélio/patologia , Epitélio , Neoplasias Epiteliais e Glandulares , Estudos Retrospectivos
16.
Actas urol. esp ; 40(1): 17-22, ene.-feb. 2016. ilus
Artigo em Espanhol | IBECS | ID: ibc-147422

RESUMO

Objetivos: En la mayoría de las biopsias prostáticas el epitelio seminal se reconoce fácilmente, ya que muestra criterios histológicos característicos. Sin embargo, algunas biopsias pueden semejar lesiones prostáticas malignas o premalignas. Los propósitos de este estudio son analizar el aspecto histológico de las biopsias que simularon adenocarcinomas o lesiones prostáticas preneoplásicas; comentar su diagnóstico diferencial y conocer la frecuencia de epitelio seminal en biopsias prostáticas. Métodos: Revisamos 500 biopsias prostáticas consecutivas por punción obtenidas por el método de sextantes, y seleccionamos aquellos casos en los que observamos epitelio seminal de vesículas seminales o conductos eyaculatorios. En las biopsias en las que el epitelio seminal semejó lesiones malignas o premalignas se emplearon estudios inmunohistoquímicos que incluyeron antígeno prostático específico y MUC6. Se anotaron los datos clínicos de mayor importancia. Resultados: Treinta y seis (7,2%) biopsias mostraron epitelio seminal y 7 de ellas (1,4%) semejaron diversas lesiones prostáticas, incluyendo neoplasia intraepitelial prostática de alto grado, proliferaciones acinares atípicas, adenocarcinomas con patrón papilar y carcinoma poco diferenciado. El epitelio seminal semejó lesiones prostáticas cuando el depósito de lipofuscina, las vacuolas perinucleares o las pseudoinclusiones nucleares fueron poco aparentes o estuvieron ausentes. Cinco de las 7 biopsias mostraron atipia celular leve o moderada con núcleos pequeños e hipercromáticos y solos 2 pleomorfismo celular. Los pacientes se encontraban vivos y asintomáticos después de 6 años de evolución en promedio. Conclusiones: El epitelio seminal semeja neoplasia intraepitelial prostática, proliferaciones acinares atípicas y diversos tipos de adenocarcinomas prostáticos en aproximadamente el 1,4% de las biopsias prostáticas


Objectives: In most prostate biopsies, the seminal epithelium is easily recognised because it meets characteristic histological criteria. However, some biopsies can mimic malignant or premalignant prostatic lesions. The aims of this study were to analyse the histological appearance of the biopsies that mimic adenocarcinomas or preneoplastic prostatic lesions, discuss the differential diagnosis and determine the frequency of seminal epithelia in prostate biopsies. Methods: We consecutively reviewed 500 prostate puncture biopsies obtained using the sextant method and selected those cases in which we observed seminal vesicle or ejaculatory duct epithelium. In the biopsies in which the seminal epithelium resembled malignant or premalignant lesions, immunohistochemical studies were conducted that included prostate-specific antigen and MUC6. The most important clinical data were recorded. Results: Thirty-six (7.2%) biopsies showed seminal epithelium, and 7 of them (1.4%) resembled various prostate lesions, including high-grade prostatic intraepithelial neoplasia, atypical acinar proliferations, adenocarcinomas with papillary patterns and poorly differentiated carcinoma. The seminal epithelium resembled prostate lesions when the lipofuscin deposit, the perinuclear vacuoles or the nuclear pseudoinclusions were inconspicuous or missing. Five of the 7 biopsies showed mild to moderate cellular atypia with small and hyperchromatic nuclei, and only 2 showed cellular pleomorphism. The patients were alive and asymptomatic after an average of 6 years of progression. Conclusions: The seminal epithelium resembles prostatic intraepithelial neoplasia, atypical acinar proliferations and various types of prostatic adenocarcinomas in approximately 1.4% of prostate biopsies


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Idoso , Adenocarcinoma/patologia , Lesões Pré-Cancerosas/patologia , Próstata/patologia , Neoplasias da Próstata/patologia , Glândulas Seminais/patologia , Biópsia por Agulha , Diagnóstico Diferencial , Epitélio/patologia
17.
Clin. transl. oncol. (Print) ; 18(2): 113-124, feb. 2016. ilus
Artigo em Inglês | IBECS | ID: ibc-148215

RESUMO

Twist proteins are members of basic helix-loop-helix family and are major regulators of embryogenesis. In adult humans, Twist proteins are mainly expressed in precursor cells, including myogenic, osteoblastic, chondroblastic and myelomonocytic lineages, maintaining their undifferentiated state. In addition, they play important roles in lymphocyte function and maturation. Recently, several studies have reported regulatory roles for Twist in the function and development of hematopoietic cells as well as in survival and development of numerous hematological malignancies. It is activated by numerous signal transduction pathways, including Akt, nuclear factor κB, Wnt, signal transducer and activator of transcription 3, mitogen-activated protein kinase and Ras signaling. Activated Twist has an anti-apoptotic role and protects cancer cells from apoptotic cell death. In addition, overexpression of Twist promotes the process of epithelial-mesenchymal transition, which has an essential role in cancer metastasis. Hereby, we review the aberrant expression of Twist in hematopoietic malignancies such as leukemias, lymphomas and myelodysplastic syndrome, which is related with poor prognosis and drug resistance in these disorders. Inactivation of Twist by small RNAs technology or chemotherapeutic inhibitors targeting Twist and upstream or downstream molecules of Twist signaling pathways may be helpful in management of disease to improve treatment strategies in malignancies (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Biomarcadores/metabolismo , Neoplasias da Medula Óssea/metabolismo , Neoplasias da Medula Óssea/patologia , Neoplasias Hematológicas/sangue , Neoplasias Hematológicas/genética , Protamina Quinase/administração & dosagem , Epitélio/anormalidades , Leucemia/sangue , Nicho de Células-Tronco/genética , Biomarcadores/análise , Neoplasias da Medula Óssea/complicações , Neoplasias Hematológicas/metabolismo , Neoplasias Hematológicas/patologia , Protamina Quinase , Protamina Quinase/metabolismo , Apoptose/fisiologia , Epitélio/patologia , Leucemia/tratamento farmacológico , Nicho de Células-Tronco/fisiologia
18.
Rev. Rol enferm ; 39(1): 49-52, ene. 2016. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-149404

RESUMO

Introducción. La epitelitis es un efecto secundario frecuente en el tratamiento con radioterapia (RT). Objetivo. Evaluar la efectividad del aceite de rosa mosqueta (ARM, RepavarR) en la prevencion de epitelitis en pacientes con cancer de cabeza y cuello (CCC) tratados con radioterapia de intensidad modulada (IMRT). Material y métodos. Estudio clinico, intervencionista, prospectivo, exploratorio, controlado y abierto. Entre mayo y octubre de 2013 se incluyeron en el estudio 28 pacientes. Tras firmar el consentimiento informado, se les proporciono ARM para aplicarselo 2 veces/dia desde el inicio del tratamiento. Todo tenian prescritas dosis de IMRT ≥ 60 Gy. Enfermeria los evaluo semanalmente. Se realizo un seguimiento fotografico de la piel que registro el grado de epitelitis y la aparicion de dolor u otros. El grupo control lo constituyeron 34 pacientes consecutivos con CCC tratados con IMRT los meses previos al estudio, cuyo tratamiento topico se realizo con otros productos. Resultados. Ambos grupos son comparables (edad, sexo, localización y dosis RT). En el grupo de estudio (ARM) fue menor el numero de curas por paciente (p = 0.3) y los dias naturales de curas por paciente (p = 0.38); tambien fue menor el numero de pacientes que preciso curas y padecio radiodermitis grado III-IV. Probablemente, el limitado tamano muestral haya influido en la significacion estadistica. Conclusiones. La efectividad del ARM en la prevencion de la epitelitis grave en pacientes con CCC tratados con RT es al menos tan eficaz (probablemente mas) que la de los tratamientos topicos habituales. Seria deseable un estudio leatorizado para confirmar dichos hallazgos (AU)


Introduction. Epithelitis is a frequent side effect in radiotherapy treatments (RT). Objective. To evaluate the effectiveness of Rosehip Oil (ARM, Repavar) in the prevention of epithelitis in patients with head and neck cancer (HNC) treated with intensity modulated radiotherapy (IMRT). Material and methods. An interventionist, prospective, exploratory, controlled and open clinical study. Between May and October 2013, 28 patients were included in the study. After signing the informed consent form, the patients were provided with ARM to apply this twice daily from the beginning of the treatment. All of them had a prescribed dose of IMRT ≥ 60 Gy. They were evaluated weekly by nurses on the ward. A photographic monitoring of the skin with a registered grade of epithelitis and with signs of pain or other was carried out. The control group was formed of 34 consecutive patients with HNC treated with IMRT months prior to the study, whose skin treatment had been different products. Results. Both groups were comparable (age, sex, region and RT dose). In the study group (ARM), the number of treatments per patient (p = 0.3) and the natural days for treatment per patient (p = 0.38) were less; also, the number of patients who required treatments and who suffered from radiodermitis of III-IV grade, were less. It is probable that the limited specimen size may have influenced in the statistics. Conclusions. The effectiveness of ARM in the prevention of severe epithelitis in patients with HNC treated with RT is, at least, as effective (or more effective) as other and more common skin treatments. A randomized study would be required to confirm these findings (AU)


Assuntos
Humanos , Masculino , Feminino , Epitélio/lesões , Epitélio/efeitos da radiação , Radioterapia/efeitos adversos , Radioterapia/enfermagem , Neoplasias de Cabeça e Pescoço/enfermagem , Radiodermatite/complicações , Radiodermatite/prevenção & controle , Radiodermatite/terapia , Rosa/imunologia , Neoplasias de Cabeça e Pescoço/complicações , Neoplasias de Cabeça e Pescoço/prevenção & controle , Neoplasias de Cabeça e Pescoço/radioterapia , Pele/lesões , Pele/efeitos da radiação , Estudos Prospectivos
19.
J. physiol. biochem ; 71(4): 823-838, dic. 2015.
Artigo em Inglês | IBECS | ID: ibc-145733

RESUMO

Transdifferentiation of alveolar epithelial type II cells (AECIIs) to type I cells (AECIs) is critical for reestablishment and maintenance of an intact alveolar epithelium. However, this process is frequently destroyed by hyperoxia treatment, which is commonly used in respiratory distress syndrome therapy in preterm infants. Wnt5a is considered to participate in this physiopathologic process, but the clear mechanisms still need to be further investigated. In this study, preterm rats and primary rat AECIIs were exposed to hyperoxia. Hematoxylin and eosin staining was used to examine the histological changes of the lungs. Real-time PCR and western blotting were used to examine Wnt5a expression and biomarkers of AECII and AECI expression. Immunohistochemistry and immunofluorescence were also used to determine the expression and location of selected biomarkers. Furthermore, AECIIs transfected with Wnt5a gene and exogenous Wnt5a were used to examine whether Wnt5a contributes to the transdifferentiation of AECIIs to AECIs. Results showed that hyperoxia inhibited the transdifferentiation of AECIIs to AECIs in vitro, which is represented by biomarkers of two types of cell that remained unchanged. In addition, Wnt5a protein expression was found to be decreased after hyperoxia exposure in vitro and in vivo. Furthermore, both the overexpression of Wnt5a and exogenous Wnt5a addition blocked the inhibitory effect of hyperoxia in vitro. In conclusion, our results suggest that the transdifferentiation of AECIIs to AECIs is impaired by hyperoxia, and this process may be associated with Wnt5a downregulation. Targeting Wnt5a may have the potential for the therapy of lung injury in preterm infants induced by hiperoxia (AU)


No disponible


Assuntos
Animais , Ratos , Proteínas Wnt/fisiologia , Hiperóxia/fisiopatologia , Células Epiteliais/fisiologia , Epitélio/fisiologia , Biomarcadores/análise , Recém-Nascido Prematuro/fisiologia
20.
Med. oral patol. oral cir. bucal (Internet) ; 20(6): e670-e677, nov. 2015. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-144698

RESUMO

Background: The aim of the study was to assess the progression of Oral Submucous Fibrosis (OSF) by investigating the correlation between clinical mouth opening and muscle-epithelial distance in tissue sections. Characterization of changes involving muscle was ascertained. Material and Methods: 50 cases and 10 controls were included in this case-control study. Interincisal mouth opening was measured and classified according to Lai et al as Group A (more than 35mm), Group B (30 to 35mm), Group C (20 to 30mm), Group D (less than 20mm). Histopathological sections were graded as very early, early, moderately advanced, advanced OSF. Muscle-epithelial distance was calculated using image analysis software. The four most common degenerative changes observed in muscles, namely fragmentation, highly eosinophilic areas with loss of striations, nucleus internalization and multiple pyknotic nuclei were also assessed. Results: Comparisons of muscle-epithelial distance were made between the clinical and histopathological groups to those of controls. The mean muscle-epithelial distance was: Group A-626.8±309.36 μm, B-827.5±549.72 μm, C-673.2±321.93 μm, D-439.9±173.84μm, Controls-1222.19 ±441.7μm. Post-hoc Bonferroni Test revealed a statistically significant reduction in the muscle-epithelial distance in Group C (p-value = 0.001) and D (p-value = 0.001) as compared to controls. The mean muscle-epithelial distance in very early, early, moderately advanced and advanced OSF was 732.73±232.81μm, 726.54±361.63 μm, 548.36±273.13 and 172.40±58.41 μm respectively. Highly significant difference in muscle-epithelial distance was seen between controls as compared to early (p-value =0.002), moderately advanced (p-value = 0.001) and advanced OSF (p-value = 0.001. Fragmentation and highly eosinophilic areas were invariably noticed in advanced OSF. Multiple pyknotic nuclei were variable with no specificity. Conclusions: Reduction in muscle-epithelial distance may prove to be a significant predictor of OSF progression. Degenerative changes must be noted while observing OSF cases, irrespective of the histopathological grade (AU)


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fibrose Oral Submucosa/diagnóstico , Fibrose Oral Submucosa/patologia , Mucosa Bucal/anatomia & histologia , Mucosa Bucal/patologia , Epitélio/anatomia & histologia , Epitélio/fisiopatologia , Estudos de Casos e Controles , Fotomicrografia/instrumentação , Fotomicrografia/métodos , Fotomicrografia
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