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1.
Med. clín (Ed. impr.) ; 154: 0-0, 2020. tab, graf
Artigo em Inglês | IBECS | ID: ibc-190152

RESUMO

INTRODUCTION AND OBJECTIVES: Common laboratory parameters are crucial in aiding coronavirus disease 2019 (COVID-19) case detection. This study aimed to determine the differences between laboratory parameters in (1) COVID-19 versus non-COVID-19 pneumonia, and (2) severe versus non-severe COVID-19 cases. METHODS: Studies were collected until March 2020, and retrieved parameters include leukocyte, neutrophil, thrombocyte, and lymphocyte counts in addition to C-reactive protein (CRP), procalcitonin (PCT) and D-dimer levels. In the presence of heterogeneity, the random-effect model (REM) was used instead of the fixed-effect model (FEM). RESULTS: Seven studies in the first analysis showed significantly lower leukocyte, neutrophil and platelet counts in COVID-19 pneumonia (SMD = -0.42, 95% CI -0.60 to -0.25, p < 0.00001, SMD = -0.23, 95%CI -0.41 to -0.06, p = 0.01, SMD = -0.54, 95% CI -0.91 to -0.16, p = 0.0005) compared to non-COVID-19 pneumonia. Twenty-six studies in the second analysis showed significantly lower lymphocyte and thrombocyte counts (SMD = -0.56, 95% CI -0.71 to -0.40, p < 0.0001, SMD = -0.32, 95% CI -0.49 to -0.15, p = 0.0002) and significantly higher leukocyte, neutrophil, D-dimer, and CRP (SMD = 0.31, 95%CI 0.07-0.56, p = 0.01; SMD = 0.44, 95% CI 0.24-0.64, p < 0.0001; SMD = 0.53, 95%CI 0.31-0.75, p < 0.00001; SMD = 0.97, 95% CI 0.70-1.24, p < 0.00001) in severe COVID-19 compared to non-severe COVID-19. CONCLUSIONS: In conclusion, thrombocyte count is key in both diagnosis and prognosis. Low leukocyte and neutrophil counts are markers of COVID-19 infection, but contrastingly higher counts indicate progressive COVID-19. And although lymphocyte, D-dimer and CRP levels did not demonstrate diagnostic value, all indicate severity of COVID-19. Confirmation of these findings should be performed in future studiesn


INTRODUCCIÓN Y OBJETIVOS: Los parámetros comunes de laboratorio son cruciales para ayudar a la detección de casos de enfermedad por coronavirus 2019 (COVID-19). Este estudio tuvo como objetivo determinar las diferencias entre los parámetros de laboratorio en: 1) COVID-19 versus neumonía no COVID-19, y 2) Casos severos versus no severos de COVID-19. MÉTODOS: Los estudios se recolectaron hasta marzo de 2020, y los parámetros recuperados incluyen recuentos de leucocitos, neutrófilos, trombocitos y linfocitos además de los niveles de proteína C reactiva (PCR), procalcitonina (PCT) y dímero-D. En presencia de heterogeneidad, se utilizó el modelo de efectos aleatorios en lugar del modelo de efectos fijos. RESULTADOS: Siete estudios en el primer análisis mostraron recuentos de leucocitos, neutrófilos y plaquetas significativamente más bajos en la neumonía por COVID-19 (SMD = -0,42; IC 95%: -0,60 a -0,25; p<0,00001; SMD = -0,23; IC 95%: -0,41 a -0,06; p = 0,01; SMD = -0,54; IC 95%: -0,91 a -0,16; p = 0,0005) en comparación con la neumonía no COVID-19. Veintiséis estudios en el segundo análisis mostraron recuentos de linfocitos y trombocitos significativamente más bajos (SMD=-0,56; IC 95%: -0,71 a -0,40; p < 0,0001; SMD = -0,32; IC 95%: -0,49 a -0,15; p=0,0002) y leucocitos, neutrófilos, dímero D y PCR significativamente más altos (SMD = 0,31; IC 95%: 0,07-0,56; p = 0,01; SMD = 0,44; IC 95%: 0,24-0,64; p < 0,0001; SMD = 0,53; IC 95%: 0,31-0,75; p < 0,00001; SMD = 0,97; IC 95%: 0,70-1,24; p < 0,00001) en COVID-19 severo en comparación con COVID-19 no severo. CONCLUSIONES: En conclusión, el recuento de trombocitos es clave tanto en el diagnóstico como en el pronóstico. Los recuentos bajos de leucocitos y neutrófilos son marcadores de infección por COVID-19, pero los recuentos contrastantemente más altos indican COVID-19 progresivo. Y aunque los niveles de linfocitos, dímero D y PCR no mostraron valor diagnóstico, todos indican la gravedad de COVID-19. La confirmación de estos hallazgos debe realizarse en futuros estudios


Assuntos
Humanos , Infecções por Coronavirus/diagnóstico , Técnicas e Procedimentos Diagnósticos/normas , Estudos de Coortes , Betacoronavirus , Prognóstico , Pneumonia Viral/diagnóstico , Técnicas e Procedimentos Diagnósticos/instrumentação , Reação em Cadeia da Polimerase , Pró-Calcitonina , Plaquetas , Curva ROC
2.
Cient. dent. (Ed. impr.) ; 16(3): 181-190, sept.-dic. 2019. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-185992

RESUMO

Introducción: La osteonecrosis de los maxilares inducida por medicamentos (MRONJ) es una reacción adversa severa causada por determinados fármacos empleados para el tratamiento del cáncer y la osteoporosis que cursa con una destrucción progresiva del hueso de los maxilares. Actualmente, no se ha establecido un protocolo de tratamiento ideal para el manejo de la enfermedad. En los últimos años, el desarrollo de los concentrados plaquetarios autólogos (CPA) y su empleo en la mejora de la cicatrización ósea en el área oral ha ido en aumento. El objetivo de este trabajo es determinar la efectividad de la aplicación de CPA en el lecho quirúrgico en pacientes con riesgo de desarrollar MRONJ o con MRONJ ya establecida. Material y métodos: Se realizó una búsqueda bibliográfica en las plataformas MEDLINE/PubMed, Science Direct y Cochrane Central Registred of Controlled Trials (CENTRAL) desde el año 2003 al 2018.Resultados: Fueron incluidos 28 artículos originales en los cuáles se trataron 299 casos con MRONJ con CPA logrando la curación en 274 (92%). Y se emplearon los CPA en la prevención de la enferme-dad en 1.970 situaciones quirúrgicas en pacientes con riesgo de MRONJ sometidos a cirugías orales y no se desarrolló la enfermedad en ninguno de los casos. Conclusiones: Debido al bajo nivel de evidencia de los estudios revisados, a la heterogeneidad entre los mismos y al tamaño muestral reducido, los resultados obtenidos no aportan evidencia científica suficiente que demuestre una ventaja objetiva en el empleo de CPA en el tratamiento y prevención de la MRONJ


Purpose: Medication related osteonecrosis of the jaws (MRONJ) is an adverse drug reaction consisting on progressive bone destruction in the maxilofacial region of patients with cancer or osteoporosis under treatment with certain drugs. The management of patients with MRONJ remains controversial as an ideal treatment protocol has not been yet established. Autologous platelet concentrates (APC) have demonstrated to improve bone healing. The aim of the present systematic review is to evaluate the effectiveness of APC’s application for treatment and prevention of MRONJ. Methods: a bibliographic research was performed on the following databases: MEDLINE/PubMed, ScienceDirect and Cochrane Central Registred of Controlled Trials (CENTRAL). Results: 28 studies were included, reporting on 299 patients affected by MRONJ were treated in combination with APC, satisfactory healing was observed in 92%. Additionaly, APC for MRONJ prevention were used in 1.970 risky patients undergoing oral surgery procedures, and none of them developed osteonecrosis.Conclusion: Although the results obtained using APC for MRONJ prevention are satisfactory, due to the low level of evidence, the heterogenety and small simple size of the studies, cannot be demonstrated that the use of APC is beneficial for the treatment and prevention of MRONJ


Assuntos
Humanos , Osteonecrose/induzido quimicamente , Osteonecrose/prevenção & controle , Fibrina Rica em Plaquetas , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/epidemiologia , Osteonecrose/terapia , Mandíbula/efeitos dos fármacos , Mandíbula/fisiopatologia , Plaquetas/metabolismo , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/diagnóstico , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/terapia
4.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 36(9): 539-543, nov. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-176829

RESUMO

OBJECTIVE: The comparison on mitochondrial function between severe septic patients and healthy control subjects according to mitochondrial deoxyribonucleic acid (mtDNA) haplogroup has not been previously reported; and this was the objective of the current study. METHODS: Prospective, multicenter, observational study. We obtained blood samples from 198 severe septic patients at days 1, 4 and 8 of severe sepsis diagnosis and from 96 sex- and age-matched healthy controls to determine mtDNA haplogroup and platelet respiratory complex IV (CIV) specific activity. The endpoint of the study was 30-day mortality. RESULTS: We included 198 severe septic patients (38 with mtDNA haplogroup JT and 160 with mtDNA haplogroup non-JT) and 96 healthy control subjects (16 with mtDNA haplogroup JT and 80 with mtDNA haplogroup non-JT). We have no found statistically significant differences in platelet CIV specific activity between healthy controls and survivor severe septic patients with mtDNA haplogroup JT at days 1, 4 and 8 of severe sepsis diagnosis; and the remaining severe septic patients showed lower platelet CIV specific activity than healthy controls with the same mtDNA haplogroup. CONCLUSIONS: The new finding of our study was that survivor severe septic patients and healthy controls with mtDNA haplogroup JT showed no different platelet Civ specific activity


OBJETIVO: La comparación en la función mitocondrial entre pacientes con sepsis grave y sujetos sanos según el haplogrupo del ácido desoxirribonucleico mitocondrial (ADNmt) no se ha reportado previamente; y este fue el objetivo del estudio. MÉTODOS: Estudio prospectivo, multicéntrico y observacional. Obtuvimos muestras sanguíneas de 198 pacientes con sepsis grave en los días 1, 4 y 8 del diagnóstico de la sepsis grave y de 96 sujetos sanos para determinar el haplogrupo del ADNmt y la actividad del complejo respiratorio mitocondrial IV (CIV) en plaquetas circulantes. La variable resultado principal del estudio fue la mortalidad a los 30 días. RESULTADOS: Se incluyeron 198 pacientes con sepsis grave (38 con haplogrupo JT del ADNmt y 160 con otro haplogrupo del ADNmt) y 96 sujetos sanos (16 con haplogrupo JT del ADNmt y 80 con otro haplogrupo del ADNmt). No encontramos diferencias estadísticamente significativas en la actividad de CIV plaquetaria entre los sujetos sanos y los pacientes sépticos supervivientes con haplogrupo JT del ADNmt en los días 1, 4 y 8 del diagnóstico de la sepsis grave; y el resto de los pacientes sépticos presentaron menor actividad de CIV plaquetaria que los sujetos sanos con su mismo haplogrupo del ADNmt. CONCLUSIONES: El nuevo hallazgo de nuestro estudio fue que los pacientes sépticos y sujetos sanos con haplogrupo JT del ADNmt no tenían diferencias en la actividad de CIV plaquetaria


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Mitocôndrias/genética , Mitocôndrias/metabolismo , Sepse/sangue , Sepse/fisiopatologia , Plaquetas , Estudos Prospectivos , Estudos de Casos e Controles , Sepse/mortalidade , Espanha/epidemiologia , Unidades de Terapia Intensiva
5.
Rev. esp. cir. oral maxilofac ; 39(2): 91-98, abr.-jun. 2017. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-161183

RESUMO

La regeneración periodontal es la reproducción o reconstitución de una parte perdida o dañada del periodonto con el fin de restaurar su arquitectura y función. En los últimos años se ha puesto de manifiesto el papel clave que juegan las plaquetas en la regeneración tisular, acelerando la cicatrización tanto de tejidos blandos como duros, mediada por la liberación de citocinas y factores de crecimiento durante un tiempo prolongado. La fibrina rica en plaquetas y leucocitos utilizada por primera vez por Choukroun en el 2001 es un concentrado de plaquetas de segunda generación que se obtiene a partir de la propia sangre del paciente, sin el empleo de aditivos, con el fin de conseguir una malla de fibrina que sirva de andamiaje para las sustancias implicadas en la regeneración. El objetivo de este trabajo es el de realizar una revisión y puesta al día en el uso de esta técnica (AU)


Periodontal regeneration is the reproduction or re-enactment of an injured, or lost, part of the periodontium, with the aim of repairing its architecture and main function. The key role of platelets in tissue regeneration has been demonstrated in the last few years. They accelerate healing in both the soft and hard tissues due to the liberation of cytokines and growth factors over a long period. Leucocyte-rich platelet-rich fibrin, used for the first time by Choukroun in 2001, is a second generation platelets extract that is obtained from the patient's own blood, without the need of additives. Its purpose is to attain an autologous fibrin mesh to be used for as a framework for the substances involved in bone regeneration. The purpose of this work is to present a review and an update on the use of this technique (AU)


Assuntos
Humanos , Masculino , Feminino , Regeneração Óssea/fisiologia , Plaquetas , Leucócitos , Periodonto/fisiologia , Tempo de Lise do Coágulo de Fibrina/métodos , Fibrina/uso terapêutico , Periodonto/crescimento & desenvolvimento , Cirurgia Bucal , Materiais Biocompatíveis/uso terapêutico , Substitutos Ósseos/uso terapêutico
6.
Rev. int. androl. (Internet) ; 14(2): 41-45, abr.-jun. 2016. tab
Artigo em Inglês | IBECS | ID: ibc-153238

RESUMO

Objective. To investigate the relationship between platelet activation parameters that are involved in the vascular response, the atherothrombotic process, and erectile function, in which epithelial dysfunction plays a significant role. Materials and methods. A study was performed on patients who had a color Doppler ultrasound (CDUS) of the penis due to erectile dysfunction. The patients were divided into two groups: those with normal CDUS findings, and those with vascular dysfunction on CDUS. Patients were also divided into two groups according to their scores using the International Index of Erectile Function (IIEF). The relationships between platelet activation factors, vascular parameters, and severity of the disorder were analyzed. Results. A total of 91 patients who fulfilled the inclusion criteria were included in the study. CDUS showed vascular dysfunction in 55 patients (Group I), while the findings were normal in 36 patients (Group II). Age, cholesterol level, disease duration, mean platelet volume (MPV), and platelet distribution width (PDW) were compared between Groups I and II, with Group I showing significantly higher values. The parameters that could affect Doppler ultrasound results were analyzed using multivariate regression analysis. This showed that PDW and disease duration were independent prognostic factors (p = .021 and p = .005, respectively). When the patients were divided into two groups according to their IIEF scores, in those with mild (Group A) and severe disease (Group B), it was found that there were significant differences between the groups with age, disease duration, and PDW, while two groups were found similar in terms of MPV, cholesterol levels, and hormone parameters. Conclusion. It is supposed that increased platelet activation parameters, and PDW in particular, give important information for disease progression and follow-up of vascular dysfunction in erectile dysfunction (AU)


Objetivo. Investigar la relación entre los parámetros de activación de plaquetas involucrados en la respuesta vascular, el proceso aterotrombótico y la función eréctil, en la que la disfunción endotelial desempeña un papel importante. Material y métodos. Se analizaron los pacientes en los que se realizó una ecografía Doppler color (EDC) del pene por disfunción eréctil. Los pacientes fueron divididos en 2 grupos: con resultados normales en la EDC y con disfunción vascular en la EDC. Los pacientes fueron asimismo divididos en 2 grupos en función de las puntuaciones del Índice Internacional de Función Eréctil (IIFE). Se analizaron las relaciones entre los factores de activación plaquetaria, los parámetros vasculares y la gravedad del trastorno. Resultados. Un total de 91 pacientes que cumplieron los criterios de inclusión fueron incluidos en el estudio. La EDC mostró disfunción vascular en 55 pacientes (grupo i), mientras que los resultados fueron normales en 36 pacientes (grupo ii). La edad, el nivel de colesterol, la duración de la enfermedad, el volumen medio de plaquetas (VMP) y la anchura de distribución de las plaquetas (ADP) se compararon entre los grupos i y ii; además, los valores fueron expresivamente más altos en el grupo i. Los parámetros que podrían afectar los resultados de la EDC se valoraron con el análisis de regresión multivariante, lo que demostró que la ADP y la duración de la enfermedad fueron factores pronósticos independientes (p = 0,021 y p = 0,005, respectivamente). Cuando los pacientes fueron divididos en 2 grupos en función de las puntuaciones IIFE, en aquellos con enfermedad leve (grupo A) y enfermedad grave (grupo B) se encontraron diferencias significativas entre los grupos con la edad, la duración de la enfermedad y la ADP, mientras que ambos grupos fueron similares en términos de VMP, de niveles de colesterol y de parámetros hormonales. Conclusión. Al parecer, el aumento de los parámetros de activación plaquetar, y especialmente la ADP, proporcionan una importante información para la progresión de la enfermedad y el seguimiento de la disfunción vascular en la disfunción eréctil (AU)


Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Plaquetas/fisiologia , Fator de Crescimento Derivado de Plaquetas/análise , Doenças Vasculares/complicações , Doenças Vasculares/diagnóstico , Disfunção Erétil/complicações , Disfunção Erétil/diagnóstico , Contagem de Plaquetas/métodos , Doenças Vasculares/fisiopatologia , Doenças Vasculares , Andrologia/métodos , Fatores de Crescimento do Endotélio Vascular/análise , Pênis/patologia , Pênis , Ultrassonografia Doppler em Cores/métodos , Ultrassonografia Doppler em Cores , Estudos Retrospectivos
7.
Eur. j. anat ; 20(2): 121-130, abr. 2016. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-152868

RESUMO

Platelets are blood cellular components involved in hemostatic processes and thrombus formation. Activation and inhibition of platelets result in an increase in morphological changes and a significant reduction in adhesion. There are several approaches towards the determination of the functional status of platelets, based on criteria such as cell adhesion, molecular changes at the cell surface, etc. In recent years, microfluidic devices have been introduced to mimic conditions proper to the vascular system, and so emulate thrombus formation in vivo. This study presents a microchip, the Thrombi Chip® , which is partially fitted with fluidic properties. This microchip has various types of micro-channels into which the platelets are inserted and, after drug treatment, the investigation is completed with the examination of the chip under an invert light microscope. For microscopy, cells were labeled with FCDA (human platelets) and Rho6G (mouse platelets). Counts and morphometric measurements of the adhered cells were carried out using digital images. To validate the results obtained with the microchip, the fractions of mice platelets were investigated with flow cytometry as well. Scanning electron microscopy was used to examine the morphological changes related to activation and inhibition in human platelets. The results show that, with this microchip, activation and inhibition of platelets can be detected. Flow cytometry studies largely confirm the microchip results. Certain variability in the results observed in human platelets is considered normal, as donors were randomized. In this respect the mouse platelets were much more uniform. Measurements with the microchip require that the sample be divided into three groups: control, activated and inhibited, resulting in a set of data, which, after respective evaluation, provides activity profiles, giving information on the status and response capacity of a sample. Such profiles could have diagnostic relevance and therefore be useful in a clinical context, for example in the monitoring of the effects of short- and long-term treatment of patients, as well as to test new drugs


No disponible


Assuntos
Humanos , Plaquetas/citologia , Técnicas Analíticas Microfluídicas/métodos , Ativação Plaquetária/fisiologia , Plaquetas/fisiologia , Hemostasia/fisiologia , Inibidores da Agregação de Plaquetas/análise , Trombose/fisiopatologia
8.
Med. hist ; 36(3): 4-15, 2016. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-157704

RESUMO

En este artículo se hace una revisión histórica, desde la mitad del siglo pasado hasta la actualidad, de la importancia de la plaqueta y de su actividad funcional en relación con la enfermedad tromboembólica. Esta revisión se inicia en la primera mitad del siglo pasado, cuando este «elemento forme» de la sangre era conocido, casi en exclusiva, por su participación en la hemostasia primaria. Sin embargo, en las décadas de los años ochenta y noventa, se convierte en un actor clave y central en la formación del trombo arterial para ser considerado, en la actualidad y los próximos años, en un factor importante, pero uno más, en la génesis y desarrollo de los procesos trombóticos. Se hace un repaso de los descubrimientos clave que han permitido esta evolución y como ello ha hecho posible el descubrimiento y desarrollo de fármacos capaces de inhibir la agregación plaquetaria, en particular el triflusal, un antiagregante plaquetario fruto de la investigación llevada a cabo en el Laboratorio Uriach. Es por este descubrimiento que la historia de la i+d en Uriach ha estado también unida, durante más de 4 décadas, a la historia evolutiva de la plaqueta y de la enfermedad tromboembólica (AU)


This article gives a historical review, from the middle of the last century to the present, of the importance of the platelet and its functional activity in thromboembolic disease. This review begins in the first half of the last century when this blood component was almost exclusively known for its role in primary haemostasis. However, in the nineteen-eighties and nineties, it becomes a key player in the formation of arterial thrombus. Currently and in the coming years it will be considered as an important factor, but just one more, in the formation and development of thrombotic processes. A review is done of the key events that have permitted this evolution and how this has enabled the discovery and development of platelet inhibitor drugs, in particular Trifusal, an anti-platelet agent obtained from research carried out by Uriach Laboratories. Due to this discovery, R&d history at Uriach has been linked, for over four decades, to the historical evolution of both the platelet and thromboembolic disease


Assuntos
Humanos , Masculino , Feminino , Adulto , Plaquetas/citologia , Embolia/sangue , Trombose/sangue , Hemostasia , Preparações Farmacêuticas/administração & dosagem , Fibrinogênio/administração & dosagem , Medicina Regenerativa/métodos , Coagulação Sanguínea , Plaquetas/metabolismo , Embolia/classificação , Trombose/diagnóstico , Hemostasia/fisiologia , Preparações Farmacêuticas/provisão & distribução , História do Século XVIII , Fibrinogênio/metabolismo , Medicina Regenerativa/classificação , Coagulação Sanguínea/fisiologia
9.
Arch. esp. urol. (Ed. impr.) ; 67(10): 848-852, dic. 2014. ilus
Artigo em Espanhol | IBECS | ID: ibc-131713

RESUMO

Objetivo: Presentar dos casos de masas renales infrecuentes, intentando llegar a su diagnóstico preoperatoriamente. Métodos: Describimos un caso remitido por Hematología por hallazgos en TAC de masas perirrenales bilaterales, que al biopsiar refieren hematopoyesis extramedular. El otro caso se estudió por disnea, apreciando en el TAC pulmones con múltiples quistes y en abdomen, masa quístico sólida perirrenal izquierda. La biopsia pulmonar nos informó de Linfangiomatosis pulmonar, con lo que obviamos la biopsia renal. Resultados: La mayoría de las masas renales sólidas son hipernefromas (85%). El resto de las masas son sarcomas, linfomas, tumores de vías infiltrantes y tumores benignos. Para su diagnóstico disponemos de la clínica y las pruebas radiológicas (ecografía, TAC, RNM y PET-TAC); pero ante hallazgos inespecíficos el diagnóstico se basará en el estudio histológico. Conclusiones: La hematopoyesis renal extramedular y el Linfangioma perirrenal son tumores raros y su diagnóstico preoperatorio es difíci (AU)


Objetive: To present two cases of infrequent renal masses, trying to achieve the diagnosis before surgery. Methods: We describe a case referred from the Department of Hematology in which bilateral perirrenal masses were described in the CT scan; after biopsy they where classified as extramedullary hematopoietic tissue. The other case was a patient presenting to the emergency room with dyspnea. CT Scan showed lungs with multiple cysts, chylothorax and a cystic-solid mass in the left perirenal space. In the lung biopsy they reported lung lymphangiomatosis, so we didn’t perform renal biopsy. Results: Most renal masses are renal carcinomas (85%). The less common diagnosis are sarcomas, lymphomas, upper urinary tract transitional cell carcinomas, metastases of other primary tumors, the Erdheim-Chester disease, the Castleman disease and benign tumors. All these diseases might show similar images in the CT scan and MRI, being the biopsy and histological study necessary for the diagnosis Conclusion: Perirenal extramedullary hematopoiesis and perirenal lymphangioma are rare diseases that need a pathologic study for their diagnosis (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Hematopoese/genética , Linfangioma Cístico/complicações , Linfangioma Cístico/diagnóstico , Plaquetas/citologia , Plaquetas/patologia , Nefrectomia , Nefrectomia/instrumentação , Hematopoese/fisiologia , Linfangioma Cístico/química , Linfangioma Cístico/induzido quimicamente , Plaquetas/classificação , Nefrectomia/métodos , Nefrectomia
10.
J. physiol. biochem ; 69(4): 879-887, dic. 2013.
Artigo em Inglês | IBECS | ID: ibc-121645

RESUMO

Clovers (Trifolium) may possess a significant therapeutic potential, but the effects of compounds from these plants on blood platelets and haemostasis have been poorly recognized. The present study was designed to evaluate the antioxidative action of extracts from three species of clovers:Trifolium pratense, Trifolium pallidum and Trifolium scabrum in the protection of human blood platelets in vitro. Platelet suspensions were pre-incubated with crude extract and phenolic fraction ofT. pratense or phenolic fractions of T. scabrum and T. pallidum, at the final concentrations of 0.5–50 ìg/ml. Then, for the induction of oxidative stress, 100 ìM peroxynitrite was added. The antioxidative activity of plant extracts was assessed by measurements of the level of 3-nitrotyrosine, thiol groups and lipid peroxidation products (hydroperoxides and thiobarbituric acid-reactive substances). Despite the significant differences in the composition of the investigated extracts, we observed antioxidative effects of all used mixtures. The presence of Trifolium extracts considerably reduced the peroxynitrite-mediated modifications of proteins and diminished peroxidation of lipids in platelets. Our results indicate on a strong antioxidative activity of the tested extracts-statistically significant effects were found even for the lowest concentrations (0.5 ìg/ml) of all extracts. This action may be useful in the protection of blood components, very susceptible to oxidative modifications. The obtained results suggest that the examined clovers are a promising source of compounds, valuable for the protection against oxidative stress-induced damage to blood platelets (AU)


Assuntos
Animais , Ratos , Antioxidantes/farmacocinética , Trifolium , Estresse Oxidativo , Plaquetas , Extratos Vegetais/farmacocinética , Modelos Animais de Doenças , Substâncias Protetoras/farmacocinética , Transtornos Plaquetários/prevenção & controle
11.
Angiología ; 65(5): 175-182, sept.-oct. 2013. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-124190

RESUMO

A pesar de su sencillez estructural, las plaquetas son células funcionalmente muy complejas debido a su capacidad para producir y liberar biomoléculas. De aquí su importancia en el desarrollo de la arteriosclerosis. Se realizó un experimento in vitro para estudiar la actividad de las plaquetas sobre la pared vascular observando los cambios en la expresión proteica del citoesqueleto en segmentos de aorta bovina incubados con plasma rico en plaquetas. Para intentar simular un estado inflamatorio (arteriosclerosis), se realizaron estas mismas determinaciones en segmentos preestimulados con factor de necrosis tumoral. Se observó una modulación de la expresión de la mayoría de las proteínas del citoesqueleto en los segmentos de aorta sana. Sin embargo, en los segmentos preestimulados el número de proteínas fue menor, pudiendo reflejar una capacidad dual de las plaquetas para alterar la contractilidad vascular en función del estado inflamatorio de la pared vascular (AU)


Despite its structural simplicity, platelets are functionally complex cells due to their ability to produce and release biomolecules. Hence its importance in the development of atherosclerosis. An in vitro experiment was conducted to study the effect of the platelets on the vascular wall by observing changes in the cytoskeletal protein expression in bovine aortic segments incubated with platelet rich plasma. With the aim of simulating an inflammatory state (atherosclerosis), these same measurements were performed on aortic segments pre-stimulated with tumour necrosis factor. We observed a modulation of the expression of most of the cytoskeletal proteins in healthy aorta segments. However, the number of modified proteins was less in pre-stimulated segments. These results may reflect a dual platelet capacity to alter vascular contractility in relation to the inflammatory condition of the vascular wall (AU)


Assuntos
Humanos , Plaquetas/fisiologia , Mediadores da Inflamação/análise , Inflamação/fisiopatologia , Proteômica/métodos , Endotélio Vascular/fisiopatologia , Aterosclerose/fisiopatologia , Proteínas do Citoesqueleto/fisiologia
13.
J. physiol. biochem ; 67(3): 391-399, sept. 2011.
Artigo em Inglês | IBECS | ID: ibc-122604

RESUMO

No disponible


Numerous plants (including clovers) have been widely used in folk medicine for the treatment of different disorders. This in vitro study was designed to examine the antioxidative effects of the clovamide-rich fraction, obtained from aerial parts of Trifolium pallidum, in the protection of blood platelets and plasma against the nitrative and oxidative damage, caused by peroxynitrite (ONOO−). Carbonyl groups and 3-nitrotyrosine in blood platelet and plasma proteins were determined by ELISA tests. Thiol groups level was estimated by using 5,5¡ä-dithio-bis(2-nitro-benzoic acid, DTNB). Plasma lipid peroxidation was measured spectrophotometrically as the production of thiobarbituric acid reactive substances. The results from our work indicate that clovamide-rich T. pallidum extract may reveal the protective properties in the prevention against oxidative stress. The presence of clovamide-rich T. pallidum extract (12.5¨C100 ¦Ìg/ml) partly inhibited ONOO−-mediated protein carbonylation and nitration. All the used concentrations of T. pallidum extract reduced lipid peroxidation in plasma. The antioxidative action of the tested extract in the protection of blood platelet lipids was less effective; the extract at the lowest final concentration (12.5 ¦Ìg/ml) had no protective effect against lipid peroxidation. The present results indicate that the extract from T. pallidum is likely to be a source of compounds with the antioxidative properties, useful in the prevention against the oxidative stress-related diseases (AU)


Assuntos
Humanos , Trifolium , Extratos Vegetais/farmacocinética , Estresse Oxidativo , Plaquetas , Modelos Animais de Doenças , Substâncias Protetoras/farmacocinética , Antioxidantes/farmacocinética
14.
Arch. med. deporte ; 28(141): 45-55, ene.-feb. 2011.
Artigo em Espanhol | IBECS | ID: ibc-102584

RESUMO

Se ha realizado una revisión de la bibliografía sobre los mecanismos de la hemostasia en relación con el ejercicio, de la que se desprende que tras el ejercicio intenso hay un estado de hipercoagulabilidad probablemente por aumento del factor VIII. Por otra parte, el ejercicio induce un acortamiento del tiempo de coagulación en sangre completa y del tiempo parcial de tromboplastina, aunque no están claros los efectos que tiene sobre los tiempos de Trombina y Protrombina ni tampoco la duración de estos efectos tras el ejercicio. Aunque no se conoce muy bien el mecanismo, ejercicios de diferente intensidad y duración inducen aumentos en la actividad del factor VIII y estos aumentos muestran una correlación positiva con la intensidad del ejercicio o con el volumen de trabajo en el caso de ejercicios de resistencia. Parece ser que el estímulo responsable este aumento de factor VIII está mediado por receptores beta-adrenérgicos. Está aceptado que el ejercicio intenso induce una importante activación de la fibrinólisis a consecuencia de la liberación del Activador Tisular del Plasminógeno a partir de células del endotelio vascular. El ejercicio extenuante induce un aumento en el recuento de plaquetas que se atribuye a la liberación por parte del bazo, médula ósea y lecho vascular pulmonar (AU)


We have performed a review of the literature about the mechanisms of haemostasis in connection with exercise. After intense exercise there is a hypercoagulable state throughout an increase in factor VIII. Moreover, exercise induces a shortening both in the whole blood clotting time and in the partial thromboplastin time, although the effects on the thrombin and prothrombin times are not clear as well as the duration of the effects after exercise. Although the mechanism is not well understood, exercises with different intensity and duration induce increases in the factor VIII activity and these increases are positively correlated with exercise intensity or with the amount of work in the case of resistance exercises. The stimulus forthis increase in factor VIII seems to be mediated by beta-adrenergic receptors. It is accepted that intense exercise induces a significant activation of fibrinolys is as a result of the release of tissue plasminogen activator from vascular endothelial cells. Strenuous exercise induces an increase in platelet count which is attributed to the release from the spleen, bone marrow and pulmonary vascular bed (AU)


Assuntos
Humanos , Hemostasia/fisiologia , Exercício Físico/fisiologia , Endotélio Vascular/fisiologia , Plaquetas/fisiologia , Coagulação Sanguínea/fisiologia
15.
Prog. obstet. ginecol. (Ed. impr.) ; 53(12): 507-512, dic. 2010.
Artigo em Espanhol | IBECS | ID: ibc-82972

RESUMO

Objetivo. Comparar los valores de micropartículas plaquetarias en eclámpsicas, preeclámpsicas y embarazadas normotensas. Material y método. Se seleccionaron pacientes que asistieron al Hospital Central “Dr. Urquinaona” (Maracaibo, Venezuela). Se incluyeron 30 pacientes con preeclampsia leve (grupo A), 30 pacientes con preeclampsia severa (grupo B) y 30 pacientes con eclampsia (grupo C). El grupo control fue seleccionado por tener edad e índice de masa corporal similar con los grupos en estudio y consistió en 35 embarazadas sanas (grupo D). Solo se incluyeron pacientes nulíparas. Las muestras de sangre se recolectaron en todas las pacientes antes del parto y en los grupos en estudio inmediatamente después del diagnóstico para la determinación de micropartículas plaquetarias. Resultados. Se encontraron diferencias estadísticamente significativas en la edad gestacional entre el grupo los grupos B y C comparado con los controles (p<0,05). Los valores más altos de micropartículas plaquetarias se observaron en el grupo de pacientes eclámpsicas junto con las preeclámpsicas severas. Se encontraron valores más bajos en las preeclámpsicas leves. Los grupos en estudio presentaron valores de micropartículas plaquetarias significativamente superiores que los controles (p<0,05). Al realizar el análisis de regresión lineal, se encontró que los factores que afectaban significativamente la concentración plasmática de micropartículas plaquetarias fueron: proteinuria en 24 horas, transaminasas, ácido úrico y recuento de plaquetas (p<0,05). Conclusiones. Los hallazgos de esta investigación y en este grupo de pacientes demuestran que las pacientes eclámpsicas y preeclámpsicas presentan valores de micropartículas plaquetarias más altos que las embarazadas normotensas (AU)


Objective. To compare platelet microparticle values in eclamptic, preeclamptic and normotensive pregnant women. Material and methods. Patients attending the Dr. Urquinaona Central Hospital in Maracaibo, Venezuela, were selected. Thirty patients with mild preeclampsia (group A), 30 with severe preeclampsia (group B) and 30 with eclampsia (group C) were included. Thirty-five healthy women with a similar age and body mass index to those in the study groups were selected as controls (group D). Only nuliparous patients were included. Blood samples were collected before delivery from all patients and immediately after diagnosis for platelet microparticle determination in the study groups. Results. Statistically significant differences were found in gestational age between groups B and C compared with the control group (P<.05). Higher platelet microparticle values were found in eclamptic patients and severe preeclamptic patients. Lower values were found in mild preeclamptic patients. Significantly higher platelet microparticle values were found in the study groups than in controls (P<.05). When linear regression was performed, the factors significantly affecting platelet microparticle values were 24-hour proteinuria, transaminase and uric acid levels and platelet count (P<.05). Conclusions. Our findings indicate that platelet microparticle values are higher in eclamptic and preeclamptic women than in normotensive pregnant women (AU)


Assuntos
Humanos , Feminino , Gravidez , Adulto , Pré-Eclâmpsia/sangue , Eclampsia/sangue , Plaquetas , Contagem de Plaquetas/métodos , Índice de Massa Corporal , Modelos Lineares , Dor Abdominal/diagnóstico , Dor Abdominal/etiologia , Análise de Variância
16.
Rev. esp. cir. ortop. traumatol. (Ed. impr.) ; 54(6): 378-382, nov.-dic. 2010. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-82344

RESUMO

Objetivo. Analizar el efecto de las plaquetas sobre el crecimiento de cartílago en los defectos articulares provocados en la rodilla ovina. Material y método. Se provocó un defecto de 4mm de diámetro y 3mm de profundidad en la tróclea femoral de ambas rodillas en corderos macho de 6 meses de edad. La distribución de los grupos fue: grupo A (n=6): el defecto de la rodilla derecha se rellenó con concentrado de plaquetas 5min después de ser activado con ClCa. Grupo B (n=6): el defecto se rellenó con colágeno y plaquetas. Material y método. Las plaquetas se obtuvieron por centrifugación de 10ml de sangre arterial obtenida de cada animal antes de la cirugía. En los defectos de la rodilla izquierda no se administraron plaquetas. Las ovejas fueron sacrificadas 10 semanas después de la cirugía. Se realizaron estudios macro y microscópicos. Resultados. En el grupo A, se observó cartílago hialino en 4 de los defectos de la rodilla derecha a las 10 semanas de la cirugía. Ninguno de los defectos de la rodilla izquierda mostró crecimiento de cartílago hialino. En el grupo B, no se observó cartílago hialino en nigún defecto. No obstante, todos los defectos presentaron mejor celularidad condral y menor fibrosis en los defectos tratados con plaquetas que en los no tratados. Conclusiones. Esta técnica para la reconstrucción con plaquetas de defectos articulares de oveja ha mostrado en nuestro estudio resultados esperanzadores que empeoran combinadas con un andamiaje de colágeno (AU)


Objective. To study the influence of platelets on cartilage growth in articular defects in the sheep knee. Material and methods. Male Rasa Aragonesa sheep (6 months) were operated under general anaesthesia. A 4mm diameter and 3mm deep defect was made in the femoral trochlea in both knees. The right knee defect was filled with platelet concentrate 5min after being activated with ClCa in group A (n=6), and similarly activated platelets + collagen scaffold in group B (n=6). Platelets were obtained by centrifuging 10ml arterial blood from the sheep prior to the surgical procedure. The left knee defect was not filled. The sheep were sacrificed 10 weeks after surgery. Macroscopic and microscopic studies were performed. Results. In group A, hyaline cartilage was observed in the right knee defect at the end of the experiment in four cases. None of the defects of the left knees showed hyaline cartilage growth. In group B, hyaline cartilage was not observed in any right knee defect. However, in group B, all sheep showed better chondral cellularity and regeneration and lower fibrosis in the defects treated with platelets than in non-treated ones. Conclusions. This technique for articular defect reconstruction with platelets has shown satisfactory results in our study. However, collagen scaffolds may decrease this positive effect (AU)


Assuntos
Animais , Masculino , Joelho/anormalidades , Joelho/fisiopatologia , Joelho de Quadrúpedes/patologia , Joelho de Quadrúpedes/cirurgia , Ovinos/cirurgia , Ovinos/anormalidades , Combinação Amoxicilina e Clavulanato de Potássio/uso terapêutico , Transtornos Plaquetários/sangue , Transtornos Plaquetários/veterinária , Plaquetas/classificação
17.
Rev. esp. cardiol. (Ed. impr.) ; 62(10): 1161-1178, oct. 2009. ilus
Artigo em Espanhol | IBECS | ID: ibc-73879

RESUMO

La aterosclerosis y los procesos trombóticos asociados a la rotura de placas vulnerables son la principal causa de eventos cardiovasculares incluyendo los síndromes coronarios agudos. Las lipoproteínas de baja densidad (LDL) desempeñan un papel clave en la patogenia del proceso aterotrombótico. Las LDL no sólo inducen una alteración en las propiedades antitrombóticas derivadas del endotelio vascular y en las propiedades contráctiles del vaso como resultado de una disminución en la disponibilidad de óxido nítrico endotelial y una activación de las vías de señalización proinflamatorias, sino que también afectan a la función e interacción de las células presentes en la lesión aterosclerótica, tanto derivadas de la sangre como residentes en la pared vascular. De hecho, las LDL infiltradas en el vaso sufren modificaciones (oxidaciones, agregación, glucosilación, etc.) que potencian sus propiedades aterogénicas. Una vez modificadas, las LDL intravasculares facilitan la formación de células espumosas derivadas de células musculares lisas y macrófagos y acrecientan la vulnerabilidad de las placas ateroscleróticas. Asimismo aumentan la trombogenicidad de las placas y la de la sangre, esto último asociado a un aumento en los niveles de factor tisular circulante y en la reactividad de las plaquetas. Esta revisión se centra en la importancia de las LDL, nativas y modificadas, en la patogenia de la aterotrombosis. Aborda estudios actuales sobre las LDL y su efecto en la función de células sanguíneas, especialmente plaquetas y células vasculares, así como sobre potenciales nuevas dianas terapéuticas (AU)


Atherosclerosis and thrombosis associated with the rupture of vulnerable plaque are the main causes of cardiovascular events, including acute coronary syndrome. Low-density lipoprotein (LDL) plays a key role in the pathogenesis of atherothrombotic processes. LDLs modify the antithrombotic properties of the vascular endothelium and change vessel contractility by reducing the availability of endothelial nitric oxide and activating proinflammatory signaling pathways. In addition, LDLs also influence the functions and interactions of cells present in atherosclerotic lesions, whether they come from the circulation or are resident in vessel walls. In fact, LDLs entering affected vessels undergo modifications (e.g. oxidation, aggregation and glycosylation) that potentiate their atherogenic properties. Once modified, these intravascular LDLs promote the formation of foam cells derived from smooth muscle cells and macrophages, thereby increasing the vulnerability of atherosclerotic plaque. Moreover, they also increase the thrombogenicity of both plaque and blood, in which circulating tissue factor levels are raised and platelet reactivity is enhanced. This review focuses on the importance of native and modified LDL for the pathogenesis of atherothrombosis. It also discusses current studies on LDL and its effects on the actions of vascular cells and blood cells, particularly platelets, and considers novel potential therapeutic targets (AU)


Assuntos
Humanos , Aterosclerose/fisiopatologia , Lipoproteínas LDL/farmacocinética , Plaquetas/fisiologia , Glicosilação , Hipercolesterolemia/complicações , Monócitos , Macrófagos , Endotélio Vascular/fisiologia , Agregação Plaquetária/fisiologia , Hipolipemiantes/efeitos adversos , Trombose/induzido quimicamente
18.
Av. diabetol ; 25(4): 320-328, jul.-ago. 2009. tab
Artigo em Espanhol | IBECS | ID: ibc-73361

RESUMO

En los últimos años, la citometría de fl ujo ha experimentado un gran desarrolloque la confi rma como una importante herramienta de trabajocon gran potencial clínico y analítico. Sus principales ventajas derivan delhecho de utilizar directamente sangre entera –sin el riesgo de activaciónartefactual que frecuentemente se produce por la manipulación de lamuestra cuando se trabaja con plaquetas lavadas– y de poder detectarsimultáneamente diversos antígenos en distintas subpoblaciones celularesbien identifi cadas. Esta metodología permite estudiar plaquetas circulantesactivadas espontáneamente y valorar el efecto in vitro y ex vivode diversos fármacos sobre la función plaquetaria en numerosas patologías.A pesar de sus ventajas, actualmente la aplicación de la citometríade flujo en el estudio de la diabetes y otras patologías metabólicovascularesestá lejos de ser un hecho habitual(AU)


In recent years flow cytometry has experienced a great development,which confirms it as an important tool to work with a greatclinical and analytical potential. Its major advantages derive from thefact that we work directly with whole blood, without the risk of artefactualactivation that often occurs by the manipulation of the samplewhen working with washed platelets. In addition, flow cytometry isable to detect simultaneously different antigens on well-defined cellsubpopulations. This methodology permit the study of circulatingspontaneously activated platelets and evaluate the effect of severaldrugs, in vitro and ex vivo, on platelet function in numerous pathologies.Despite its advantages, flow cytometry is currently not usedusually in the study of diabetes and other metabolic-vascular pathologies(AU)


Assuntos
Humanos , Citometria de Fluxo , Ativação Plaquetária/fisiologia , Diabetes Mellitus/fisiopatologia , Testes de Função Plaquetária , Plaquetas , Doenças Metabólicas/fisiopatologia , Complicações do Diabetes , Hipertensão/complicações , Dislipidemias/complicações
19.
J. physiol. biochem ; 65(2): 147-156, abr.-jun. 2009. graf
Artigo em Inglês | IBECS | ID: ibc-75575

RESUMO

Thrombotic diseases or fatalities have been reported to occasionally occur underconditions of hypergravity although the mechanism is still unclear. To investigate theeffect of hypergravity on platelets that are the primary players in thrombus formation,platelet rich plasma (PRP) or washed platelets were exposed to hypergravity at8 G for 15 minutes. No platelet aggregation was induced by 8 G alone, whereas ristocetinor collagen-induced platelet aggregation was significantly increased. Thenumber of platelets adherent to immobilized fibrinogen and the area of plateletsspreading on von Willbrand factor (VWF) matrix were increased simultaneously.Flow cytometry assay indicated that integrin áIIbâ3 was partially activated in 8 Gexposedplatelets, but there was no significant difference in P-selectin surface expressionbetween platelets treated with 8 G and 1 G control. The results indicate thathypergravity leads to human platelet hyperactivity, but fails to incur essential plateletactivation events, suggesting a novel mechanism for thrombotic diseases occurringunder hypergravitional conditions(AU)


Assuntos
Animais , Ratos , Hipergravidade , Hipercinese , Plaquetas , Trombose , Trombose/epidemiologia , Trombose/etiologia , Trombose/terapia , Integrina alfaVbeta3 , Integrina alfaVbeta3/uso terapêutico , Ristocetina
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