Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 84
Filtrar
Mais filtros










Filtros aplicados

Base de dados
Intervalo de ano de publicação
1.
Ars pharm ; 60(4): 227-230, oct.-dic. 2019. ilus
Artigo em Espanhol | IBECS | ID: ibc-188486

RESUMO

La clasificación oficial de la Organización Mundial de la Salud de los tumores de tejidos hematopoyéticos y linfoides de 2016 introduce el concepto de policitemia vera (PV) enmascarada y revela que esta entidad ha sido infradiagnosticada en el pasado. Se presenta el caso de un varón de 49 años, fumador, intervenido de fractura de tobillo hace más de 15 años, remitido para valorar un posible proceso infeccioso asociado. Al no producirse separación previa de los hematíes por sedimentación durante el procedimiento de marcaje de leucocitos con 99mTc-exametazima se revisaron las causas de disminución de la velocidad de sedimentación globular. Entre ellas destacan la poliglobulia y el hábito tabáquico, ambas presentes en el paciente. Se recomendó realización de estudio hematológico que concluyó con el diagnóstico de PV. Las indicaciones del especialista en Radiofarmacia permitieron diagnosticar un caso no identificado hasta entonces, pese a que el paciente presentaba síntomas desde hacía años


The official World Health Organization classification of hematopoietic and lymphoid tissue tumors introduces in 2016 the concept of masked polycythemia vera (PV) and reveals that this entity has been underdiagnosed in the past. We present the case of a 49-year-old man, smoker, operated on for ankle fracture more than 15 years ago, referred to evaluate a possible associated infectious process. As there was no previous separation of the red blood cells by sedimentation during the leukocyte labelling procedure with 99mTc-exametazima, the causes of decreased erythrocyte sedimentation rate were reviewed. These include polyglobulia and smoking, both present in the patient. A haematological study was advised, which concluded with the diagnosis of PV. The indications of the specialist in Radiopharmacy allowed diagnosing a case not identified until then, although the patient had had symptoms for years


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Leucócitos/efeitos dos fármacos , Tecnécio Tc 99m Exametazima/farmacologia , Policitemia Vera/diagnóstico por imagem , Sedimentação Sanguínea/efeitos dos fármacos , Tecnécio Tc 99m Exametazima/efeitos da radiação , Sedimentação Sanguínea/efeitos da radiação , Policitemia Vera/etiologia , Osteomielite/diagnóstico por imagem , Achados Incidentais , Cintilografia/métodos
2.
Rev. esp. med. nucl. imagen mol. (Ed. impr.) ; 38(4): 212-217, jul.-ago. 2019. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-188691

RESUMO

OBJETIVO: La gammagrafía con leucocitos marcados con 99mTc-HMPAO ha resultado un método de estudio específico y altamente sensible en el diagnóstico y seguimiento de pacientes con sospecha de infección protésica. El objetivo de este estudio retrospectivo fue evaluar la utilidad de la SPECT/TC en infección protésica. MATERIAL Y MÉTODOS: Se realizó una gammagrafía con leucocitos marcados con 99mTc-HMPAO a 37 pacientes con prótesis articular (11 hombres y 26 mujeres; rango de edades de 38 a 84 años; edad media+/-DE, 65,7+/-5,6 años). Se obtuvieron imágenes planares 2, 4 y 24horas después de la inyección. A las 4horas se realizó SPECT/TC usando una gammacámara híbrida de doble detector con TC de baja energía. En todos los pacientes se compararon los resultados de la gammagrafía con los resultados de la intervención quirúrgica, el cultivo y el seguimiento clínico. RESULTADOS: De 37 pacientes estudiados con leucocitos marcados con 99mTc-HMPAO, con gammagrafía y SPECT/TC, 17 (45,9%) tenían infección protésica y 20 (54,1%) tenían afecciones protésicas no infecciosas. La gammagrafía con leucocitos marcados con 99mTc-HMPAO fue verdadero positivo para infección en 16 de 37 pacientes y verdadero negativo en 20 de 37 pacientes. La SPECT/TC proporciona una localización anatómica precisa de todos los posibles focos. Considerando el diagnóstico final, la SPECT/TC aportó una contribución clínica significativa en 22 de 37 pacientes (59,4%). La sensibilidad, especificidad, valor predictivo negativo y valor predictivo positivo fueron del 100%, 59,1%, 100%, 62,5% en las imágenes planares y de 100%, 90,1%, 100%, 88,2% en la imagen planar más SPECT/TC respectivamente. DISCUSIÓN: Nuestros resultados indican que la realización de la SPECT/TC puede mejorar el estudio con leucocitos marcados con 99mTc-HMPAO en pacientes con sospecha de osteomielitis, y proporcionar una localización anatómica y una definición precisa del alcance de la infección


OBJECTIVES: White blood cell scanning with 99mTc-hexamethylpropylene amine oxime (HMPAO) has proven a sensitive and specific imaging method in the diagnosis of suspected prosthesis infection. The aim of this retrospective study was to evaluate the usefulness of SPECT/CT performed simultaneously using a hybrid imaging device of prosthesis infections. MATERIALS AND METHODS: 99mTc-HMPAO scintigraphy was performed on 37 patients (11 men and 26 women; age range 38-84 years; mean age+/-SD, 65.7+/-5.6 years). Planar scans were acquired 2.4 and 24hour after injection. SPECT/CT was obtained 4 h after injection, using a dual-head hybrid gama camera coupled with a low-power x-ray tube. In all patients, scintigraphic results were matched with the results of surgery, cultures and clinical follow-up. RESULTS: Seventeen (45,9%) out of 37 patients had prosthesis infection and 20 (54,1%) out of 37 patients had non-infectious prosthesis pathologies with 99mTc-HMPAO scintigraphy and SPECT/CT. The 99mTc-HMPAO scintigraphy was true-positive for infection in 16 of 37 patients and true-negative in 20 of 37 patients. SPECT/CT provided an accurate anatomic localization of all positive foci. With regard to the final diagnosis, SPECT/CT added a significant clinical contribution in 22 of 37 patients (59,4%). Sensitivity, specificity, negative predictive value and positive predictive values were 100%,59.1%,100%,62.5% in planar images with 99mTc-HMPAO scintigraphy and 100%,90.1%,100%,88.2% in the planar+SPECT/CT imaging, respectively. DISCUSSION: Our results indicate that SPECT/CT performed using a hybrid device can improve imaging with 99mTc-HMPAO scintigraphy in patients with suspected osteomyelitis by providing accurate anatomic localization and precise definition of the extent of infection


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Prótese de Quadril/efeitos adversos , Prótese do Joelho/efeitos adversos , Leucócitos , Osteomielite/diagnóstico por imagem , Infecções Relacionadas à Prótese/diagnóstico por imagem , Tomografia Computadorizada com Tomografia Computadorizada de Emissão de Fóton Único/métodos , Infecção da Ferida Cirúrgica/diagnóstico por imagem , Quimiotaxia de Leucócito , Complicações Pós-Operatórias/diagnóstico por imagem , Valor Preditivo dos Testes , Diagnóstico Diferencial , Osteomielite/etiologia , Estudos Retrospectivos , Sensibilidade e Especificidade , Tecnécio Tc 99m Exametazima
3.
Allergol. immunopatol ; 47(2): 185-193, mar.-abr. 2019. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-180808

RESUMO

Introduction: Asthma is a chronic inflammatory disease of the airways. In this study, we evaluated the anti-inflammatory effects of myrtenol on the inflammatory indices in the pulmonary parenchyma and airways and on the inflammatory and oxidative indices of the bronchoalveolar lavage fluid (BALF) of asthmatic rats. Methods: The allergic asthma was induced by sensitization (two weeks) followed by the inhalation of ovalbumin (four weeks). Animals were divided into two main groups: (1) Histopathology, and (2) measurement of inflammatory and oxidative biomarkers in the BALF. Each main group was subdivided into four subgroups: Control, Asthma, Asthma+Dexamethasone and Asthma + Myrtenol. (-)-Myrtenol (50 mg/kg) or Dexamethasone (2.5 mg/kg) was administered intraperitoneally once a day for one week, at the end of the inhalation period. On day 50, lung histopathologic parameters and inflammatory indices in BALF including INF-gamma, IL-10, IL-1Beta, and TNF-alfa and oxidative stress biomarkers (MDA, SOD, and GPX) were measured. Result: In the Asthma group, leukocyte infiltration, the thickness of smooth muscle and epithelium of airways wall and the number of goblet cells increased. Myrtenol reduced all of the above-mentioned indices except the epithelium thickness. It also inhibited the increase in BALF IL-1Beta, TNF-alfa and MDA and increased the levels of INF-gamma, IL-10 and SOD. Conclusion: Our results suggest that myrtenol reduced damage caused by experimental asthma by reducing the inflammatory indices, normalizing the level of interleukins and balancing oxidative stress in the lungs. It also prevented airway remodeling. Myrtenol may be suggested as a potent herbal medicine for the treatment of allergic asthma


No disponible


Assuntos
Humanos , Animais , Masculino , Remodelação das Vias Aéreas/efeitos dos fármacos , Anti-Inflamatórios/uso terapêutico , Asma/tratamento farmacológico , Células Caliciformes/patologia , Leucócitos/imunologia , Pulmão/imunologia , Mucosa Respiratória/patologia , Monoterpenos/uso terapêutico , Movimento Celular , Células Cultivadas , Citocinas/metabolismo , Modelos Animais de Doenças , Mediadores da Inflamação/metabolismo , Ratos Wistar , Mucosa Respiratória/efeitos dos fármacos
4.
Rev. esp. salud pública ; 93: 0-0, 2019. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-189554

RESUMO

OBJETIVO: En la literatura científica no están claros los impactos de la plaquetoféresis sobre los parámetros del leucograma, y existe una gran divergencia en los estudios que han evaluado el recuento de leucocitos. El objetivo de este estudio fue metanalizar el efecto de la plaquetoféresis sobre el recuento de leucocitos, con base en estudios publicados entre 1980-2018. MÉTODOS: Se realizó una revisión sistemática con metaanálisis de efectos aleatorios para la diferencia de medias. Se aplicaron las fases de la guía PRISMA, con 132 estrategias de búsqueda en Pubmed, Scielo, Science Direct y Scopus. Se garantizó la reproducibilidad y evaluación de la calidad metodológica. Se evaluó la heterogeneidad con Galbraith y Dersimonian y Laird's, el sesgo de publicación con Funnel Plot y Begg, y se realizaron análisis de sensibilidad, metaanálisis acumulado y Forest Plot. RESULTADOS: Se incluyeron 19 estudios con 2.358 donantes, la mayoría de India, Estados Unidos, Turquía, Alemania y Austria. Se halló una diferencia de medias de -0,80 x109⁄L (IC95%= -1,96; 0,36x109⁄L) entre el valor pre donación y el valor inmediatamente posterior a la donación. No se encontró sesgo de publicación en los estudios y la medida combinada presentó buena sensibilidad, dado que no varió con la eliminación de estudios específicos. CONCLUSIONES: La donación de plaquetas por aféresis no afecta al recuento de leucocitos en los donantes. En el siglo pasado, se reportaban reducciones en este parámetro, explicadas por la pérdida de sangre en los estuches utilizados para el procedimiento y debido a los efectos producidos en las células sanguíneas por los biomateriales de los estuches. Sin embargo, en la actualidad se evidencia la alta seguridad de la plaquetoféresis para el recuento de glóbulos blancos


BACKGROUND: In the scientific literature, the impacts of plateletpheresis on leukogram parameters are not clear, with a high divergence in the studies that have evaluated changes in leukocytes. The objective of this paper was to meta-analyze the effect of plateletpheresis on the leukocyte count, based on studies published between 1980-2018. METHODS: Systematic review with meta-analysis of random effects for the difference of means. The phases of the PRISMA guide were applied with 132 search strategies in Pubmed, Scielo, Science direct and Scopus. Reproducibility and evaluation of methodological quality were guaranteed. Heterogeneity was evaluated with Galbraith and Dersimonian and Laird's, publication bias with Funnel Plot and Begg; sensitivity analysis, accumulated meta-analysis and Forest Plot were carried out. RESULTS: Nineteen studies were included with 2,358 donors, mostly from India, United States, Turkey, Germany and Austria. A mean difference of -0,80 x109⁄L (IC95%= -1,96; 0,36x109⁄L) between the predonation value and the value immediately after donation was founded; no publication bias was found and the conclusion presented good sensitivity since it does not vary with the elimination of studies in successive phases. CONCLUSIONS: The donation of platelets by apheresis does not affect the leukocyte count in the donors, in the last century there were reports of reductions in this parameter, explained by the blood loss in the cases used for the procedure and by effects produced in the blood cells by the biomaterials; however, at present the high safety of plateletpheresis for the white blood cell count is evidenced


Assuntos
Humanos , Contagem de Células Sanguíneas , Leucócitos/citologia , Contagem de Plaquetas , Plaquetoferese/métodos , Áustria , Materiais Biocompatíveis , Índia , Reprodutibilidade dos Testes , Turquia , Estados Unidos , Doadores de Sangue , Alemanha
5.
Rev. esp. patol. torac ; 30(4): 216-223, dic. 2018. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-182314

RESUMO

INTRODUCCIÓN: El origen de la inflamación sistémica en la EPOC sigue estando poco aclarada. Hemos tratado de comparar la expresión de los niveles de reactantes de fase aguda en pacientes con EPOC y los fumadores sanos (controles), tanto en las arterias pulmonares locales como en leucocitos de sangre periférica y plasma. MÉTODO: Los pacientes se clasificaron como EPOC o fumadores sanos según los resultados de la espirometría. Las arterias pulmonares, leucocitos de sangre periférica y las muestras de plasma se obtuvieron de todos los sujetos incluidos. La expresión génica de la proteína C-reactiva (PCR) y el amiloide sérico A (AAS1, AAS2, y AAS4) se evaluó en muestras de tejidos y leucocitos de sangre periférica mediante RT-PCR. Los niveles de PCR y AAS se midieron mediante ensayos inmunoenzimáticos. RESULTADOS: Se incluyeron un total de 40 pacientes con EPOC y 62 fumadores sanos, reclutados desde comienzos del año 2011 hasta finales del 2012. Tanto PCR y AAS se sobreexpresa en la arteria pulmonar en comparación con los leucocitos de sangre periférica. En los pacientes con EPOC, en la arteria pulmonar, los niveles de expresión génica de la PCR, el AAS1, y el AAS2 fueron 8-, 56-, y 2,3 veces mayor que en los leucocitos de sangre periférica, respectivamente. No se observó correlación entre los niveles plasmáticos de marcadores inflamatorios y su expresión en la arteria pulmonar y leucocitos de sangre periférica. CONCLUSIONES: La sobreexpresión de AAS en las arterias pulmonares en comparación con los leucocitos de sangre periférica sugiere que el pulmón puede ser la principal fuente de AAS en pacientes con EPOC. Otras fuentes potenciales de inflamación sistémica en la EPOC (por ejemplo, el hígado) necesitan un examen más detallado


INTRODUCTION: The origin of systemic inflammation in COPD is still not completely clear. We have attempted to compare the expression levels of acute phase reactants in patients with COPD and healthy smokers (controls), both in the local pulmonary artery and in peripheral blood leukocytes and plasma. METHOD: Patients were classified as having COPD or being healthy smokers according to spirometry results. The pulmonary artery, peripheral blood leukocyte and plasma samples were obtained from all included subjects. Genetic expression of the C-reactive protein (CRP) and serum amyloid A (SAA1, SAA2 and SAA4) was evaluated in tissue samples and peripheral blood leukocytes using RT-CRP. CRP and SAA levels were measured using immunoenzymatic assays. RESULTS: A total of 40 patients with COPD and 62 healthy smokers were included, recruited between early 2011 and late 2012. Both CRP and SAA was overexpressed in the pulmonary artery in comparison with peripheral blood leukocytes. In patients with COPD, genetic expression levels of CRP, SAA1 and SAA2 in the pulmonary artery were 8, 56, and 2.3 times higher than in peripheral blood leukocytes, respectively. No correlation was observed between inflammatory marker plasma levels and their expression in the pulmonary artery and peripheral blood leukocytes. CONCLUSIONS: The overexpression of SAA in pulmonary arteries in comparison with peripheral blood leukocytes suggests that the lung may be the main source of SAA in patients with COPD. Other potential sources of systemic inflammation in COPD (for example, the liver) require more detailed examination


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Idoso , Artéria Pulmonar/diagnóstico por imagem , Doença Pulmonar Obstrutiva Crônica/diagnóstico por imagem , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Artéria Pulmonar/anatomia & histologia , Leucócitos , Proteína Amiloide A Sérica/análise , Reação em Cadeia da Polimerase , Espirometria , Inquéritos e Questionários , Fumantes/estatística & dados numéricos
6.
Nutr. hosp ; 35(3): 570-575, mayo-jun. 2018. graf, tab
Artigo em Inglês | IBECS | ID: ibc-180113

RESUMO

Introduction: inflammation and oxidative stress are factors that may play a substantial role in telomere attrition. In line of this, obesity is associated with telomere shortening. Green tea had anti-inflammatory and antioxidant effects and may alter telomere length (TL).Objectives: we evaluated the effect of decaffeinated green tea supplementation in obese women on TL. Methods: we conducted a cross-sectional interventional study with ten obese (body mass index [BMI] > 40 kg/m²) and eight normal weight (BMI > 18.5 and < 24.9 kg/m²) women (age between 27 and 48 years). The supplementation was carried out with capsules (each contained 450.7 mg of epigallocatechin-3-gallate) during eight weeks. Anthropometric and dietary intake assessment, and blood collection (for biochemical and TL analysis by quantitative PCR) were performed before and after supplementation. Normal weight patients were evaluated at a single moment. Results: we observed a significant increase on TL after supplementation (1.57 ± 1.1 to 3.2 ± 2.1 T/Sratio; p < 0.05). Moreover, we found shorter TL in obese patients (day 0) when compared to normal weight individuals (3.2 ± 1.9 T/Sratio; p < 0.05) and an inverse association between TL and BMI, even after age adjustment (beta = -0.527; r² = 0.286; IC = -0.129, -0.009).Conclusion: obesity is related to shorter telomeres. Green tea supplementation during eight weeks promotes telomere elongation in obese women


Introducción: la inflamación y el estrés oxidativo son factores que pueden jugar un papel importante en el desgaste de los telómeros. En línea con esto, la obesidad está asociada con el acortamiento de los telómeros. El té verde tiene efectos antiinflamatorios y antioxidantes y puede alterar la longitud de los telómeros (LT). Objetivos: evaluamos el efecto de la suplementación de té verde descafeinado en la LT en mujeres obesas. Métodos: realizamos un estudio intervencionista de corte transversal con 10 mujeres obesas (IMC > 40 kg/m²) y 8 con peso normal (IMC > 18,5 y < 24,9 kg/m²) (edad entre 27 y 48 años). La suplementación se llevó a cabo con cápsulas (cada una contenía 450,7 mg de epigalocatequina- 3-galato) durante 8 semanas. La evaluación de la ingesta antropométrica y dietética y la recolección de sangre (para análisis bioquímicos y LT por PCR cuantitativa) se realizaron antes y después de la administración de suplementos. Los pacientes de peso normal fueron evaluados en un solo momento. Resultados: observamos un aumento significativo en LT después de la suplementación (1,57 ± 1,1 a 3,2 ± 2,1 T/S ratio; p < 0,05). Además, encontramos LT más corta en pacientes obesos (día 0) en comparación con individuos de peso normal (3,2 ± 1,9 T/S ratio; p < 0,05) y una asociación inversa entre LT e IMC, incluso después del ajuste de edad (beta = -0,527; r² = 0,286; IC = -0,129, -0,009). Conclusión: la obesidad está relacionada con los telómeros más cortos. La administración de suplementos de té verde durante 8 semanas promueve la elongación de los telómeros en mujeres obesas


Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Catequina/análogos & derivados , Suplementos Nutricionais , Leucócitos/ultraestrutura , Obesidade/dietoterapia , Chá , Telômero/ultraestrutura , Índice de Massa Corporal , Catequina/farmacologia , Estudos Transversais , Leucócitos , Obesidade/sangue , Telômero , Encurtamento do Telômero
7.
Rev. psiquiatr. salud ment. (Barc., Ed. impr.) ; 11(2): 94-100, abr.-jun. 2018. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-174309

RESUMO

Material y métodos. Se estudió una muestra de mujeres ingresadas en nuestra UCPP y en tratamiento con clozapina. Se estudió la variación de los recuentos de leucocitos y neutrófilos durante las primeras 18 semanas de tratamiento, la aparición de leucopenia, neutropenia y agranulocitosis, así como la influencia de los fármacos empleados de forma concomitante. Resultados y conclusiones. Se obtuvo una tasa de incidencia de neutropenia de 1,82% (IC 95%: 0,05-10,13) y ningún caso de leucopenia ni agranulocitosis (0%). En el análisis cuantitativo de leucocitos y neutrófilos durante las 18 semanas de tratamiento, se observó un aumento hasta la semana 3-4, tendiendo después a la estabilización de las cifras alcanzadas, pero manteniendo siempre cifras superiores a las de los valores iniciales. Estas diferencias resultaron estadísticamente significativas para los leucocitos en el ANOVA de medidas repetidas con la corrección de Greenhouse-Geisser F (11,47, 37)=2,114 (p=0,011), ŋ2P=0,038. También resultó significativo para los neutrófilos el ANOVA con la corrección de Greenhouse-Geisser F (10,33, 37)=3,312 (p=0,0002), y el MANOVA F (18, 37)=2,693 (p=0,005), ŋ2P=0,567). La influencia de los fármacos estudiados de forma concomitante (litio, valproico y biperideno) no resultó globalmente significativa (MANOVA) sobre el aumento hallado en los leucocitos y neutrófilos


Introduction. Clozapine is an antipsychotic drug that has shown to be more effective than other antipsychotics in the treatment of schizophrenia, but its use is limited due to its side effects, particularly by the risk of causing agranulocytosis. A study was made on the variations in white cell and neutrophil counts in patients treated with clozapine in a Long-term Psychiatric Unit. Methods. A retrospective observational study was conducted with a sample of women of our long-term psychiatric care unit who had been treated with clozapine. A study was made on the variations in white cell and neutrophil counts during the first 18 weeks of treatment, as well as the onset of leukopenia, neutropenia, agranulocytosis, and the influence of concomitant drugs. Results and conclusions. The study included 55 patients on treatment with clozapine. The incidence rate of neutropenia was 1.82% (95% CI; 0.05-10.13). The incidence rate of leukopenia and agranulocytosis was 0%. An increase in white cell and neutrophil counts from baseline to week 3-4 was observed. Only small variations were observed after this time, but the counts remained higher than the initial values. These changes were statistically significant in the white cell count: One-way repeated ANOVA with Greenhouse-Geisser correction F (11.47, 37) = 2.114 (P= .011); and in neutrophils: One-way repeated ANOVA with Greenhouse-Geisser correction F (10.3, 37)=3.312 (P=.0002), and MANOVA F (18, 37)=2.693 (P=.005), ŋ2P=0.567. The influence of concomitant drugs (lithium, valproic and biperiden) was not significant on the overall increase found in white cells or neutrophils (MANOVA)


Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Contagem de Leucócitos/métodos , Transtornos Leucocíticos/tratamento farmacológico , Clozapina/administração & dosagem , Ativação de Neutrófilo , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/complicações , Leucócitos , Neutrófilos , Clozapina/metabolismo , Clozapina/farmacologia , Análise de Variância , Agranulocitose/induzido quimicamente , Intervalos de Confiança , Leucopenia/induzido quimicamente
9.
Rev. esp. med. nucl. imagen mol. (Ed. impr.) ; 36(6): 388-391, nov.-dic. 2017. ilus
Artigo em Espanhol | IBECS | ID: ibc-167313

RESUMO

La infección de injertos vasculares es una complicación poco frecuente en este tipo de procedimientos. Sin embargo, cuando se presentan suelen tener una alta morbilidad e incluso una alta mortalidad. Su adecuada identificación y localización es crucial para el pertinente y temprano manejo, ya sea médico o quirúrgico, por lo que el conocimiento de las herramientas adecuadas es primordial. Los estudios de medicina nuclear tienen un importante papel en este sentido, ya sea mediante gammagrafía con leucocitos marcados o con 18F-FDG. La elección dependerá de la experiencia con ambas técnicas de los diferentes grupos, del conocimiento de las mismas y de su disponibilidad, entre otros factores. Presentamos 2 casos en los que la gammagrafía con 99mTc-HMPAO-leucocitos autógenos marcados-SPECT/TC fue de gran utilidad en el diagnóstico y localización del compromiso infeccioso sospechado y, por lo tanto, en el adecuado y guiado manejo posterior. Ambos casos tuvieron confirmación clínica y microbiológica (AU)


Vascular graft infections are a rare complication in this type of procedure. However, when they do occur, they usually have high morbidity, and even a high mortality. Proper identification and location is crucial for the appropriate and early management, whether medical or surgical, thus knowledge of the right tools is paramount. Nuclear medicine studies play an important role in this regard, either by using labelled white blood cells scintigraphy or 18F-FDG. The choice, among other factors, will depend on the experience with both techniques by the different groups, their knowledge of them, as well as their availability. Two cases are presented in which 99mTc-HMPAO-white blood cells SPECT/CT scintigraphy was very useful in the diagnosis and location of the suspected infectious compromise, which led to the subsequent appropriate and guided management. Both cases were confirmed clinically and microbiologically (AU)


Assuntos
Humanos , Masculino , Idoso , Idoso de 80 Anos ou mais , Enxerto Vascular , Cintilografia , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Leucócitos/efeitos da radiação , Infecções Estafilocócicas , Medicina Nuclear/métodos , Tecnécio/administração & dosagem , Staphylococcus aureus/isolamento & purificação , Doenças Vasculares
10.
J. physiol. biochem ; 73(3): 445-455, ago. 2017. graf, tab
Artigo em Inglês | IBECS | ID: ibc-178895

RESUMO

Epigenetic processes, including DNA methylation, might be modulated by environmental factors such as the diet, which in turn have been associated with the onset of several diseases such as obesity or cardiovascular events. Meanwhile, Mediterranean diet (MedDiet) has demonstrated favourable effects on cardiovascular risk, blood pressure, inflammation and other complications related to excessive adiposity. Some of these effects could be mediated by epigenetic modifications. Therefore, the objective of this study was to investigate whether the adherence to MedDiet is associated with changes in the methylation status from peripheral blood cells. A subset of 36 individuals was selected within the Prevención con Dieta Mediterránea (PREDIMED)-Navarra study, a randomised, controlled, parallel trial with three groups of intervention in high cardiovascular risk volunteers, two with a MedDiet and one low-fat control group. Changes in methylation between baseline and 5 years were studied. DNA methylation arrays were analysed by several robust statistical tests and functional classifications. Eight genes related to inflammation and immunocompetence (EEF2, COL18A1, IL4I1, LEPR, PLAGL1, IFRD1, MAPKAPK2, PPARGC1B) were finally selected as changes in their methylation levels correlated with adherence to MedDiet and because they presented sensitivity related to a high variability in methylation changes. Additionally, EEF2 methylation levels positively correlated with concentrations of TNF-alfa and CRP. This report is apparently the first showing that adherence to MedDiet is associated with the methylation of the reported genes related to inflammation with a potential regulatory impact


No disponible


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Diabetes Mellitus Tipo 2/dietoterapia , Dieta Mediterrânea , Leucócitos/metabolismo , Transcriptoma/genética , Proteína C-Reativa/genética , Proteína C-Reativa/metabolismo , Metilação de DNA , Diabetes Mellitus Tipo 2/metabolismo , Epigênese Genética , Inflamação/genética , Inflamação/metabolismo , Molécula 1 de Adesão Intercelular/genética , Molécula 1 de Adesão Intercelular/metabolismo
11.
J. physiol. biochem ; 73(3): 465-474, ago. 2017. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-178897

RESUMO

Epigenetics has an important role in the regulation of metabolic adaptation to environmental modifications. In this sense, the determination of epigenetic changes in non-invasive samples during the development of metabolic diseases could play an important role in the procedures in primary healthcare practice. To help translate the knowledge of epigenetics to public health practice, the present study aims to explore the parallelism of methylation levels between white blood cells and buccal samples in relation to obesity and associated disorders. Blood and buccal swap samples were collected from a subsample of the Spanish cohort of the Food4Me study. Infinium HumanMethylation450 DNA Analysis was carried out for the determination of methylation levels. Standard deviation for Beta values method and concordance correlation analysis were used to select those CpG which showed best parallelism between samples. A total of 277 CpGs met the criteria and were selected for an enrichment analysis and a correlation analysis with anthropometrical and clinical parameters. From those selected CpGs, four presented high associations with BMI (cg01055691 in GAP43; r = -0.92 and rho = -0.84 for blood; r = -0.89 and rho = -0.83 for buccal sample), HOMA-IR (cg00095677 in ATP2A3; r = 0.82 and rho = -0.84 for blood; r = -0.8 and rho = -0.83 for buccal sample) and leptin (cg14464133 in ADARB2; r = -0.9182 and rho = -0.94 for blood; r = -0.893 and rho = -0.79 for buccal sample). These findings demonstrate the potential application of non-invasive buccal samples in the identification of surrogate epigenetic biomarkers and identify methylation sites in GAP43, ATP2A3 and ADARB2 genes as potential targets in relation to overweight management and insulin sensibility


No disponible


Assuntos
Humanos , Masculino , Feminino , Adulto , Metilação de DNA , Mucosa Bucal/metabolismo , Sobrepeso/metabolismo , Ilhas de CpG , Epigênese Genética , Leucócitos/metabolismo , Sobrepeso/genética
12.
Allergol. immunopatol ; 45(4): 325-332, jul.-ago. 2017. tab, graf
Artigo em Inglês | IBECS | ID: ibc-165091

RESUMO

Objective: Infants are born immunologically immature. However, breastfeeding mothers retain an immunological link to their infants. While it is generally accepted that infants are at an immunological advantage when compared with formula-fed infants, the benefit of long-term exclusive breastfeeding by atopic mothers remains controversial. Inconsistency in the conferral of benefit may be due to differences in the immunological constituents passed to the recipient infant. The aim of this investigation was to examine the profile of human milk cells and cytokines from asthmatic compared to non-asthmatic mothers. Methods: Twenty-five exclusively breastfeeding mothers with a clinical diagnosis of asthma were postpartum age matched in a double-control 2:1 design with 50 non-asthmatic controls. Each mother provided a single milk sample which was assayed for cell differential by flow cytometry, for ex vivo cytokine production in culture and for aqueous phase cytokines. Results: Milks from asthmatic mothers differed from non-asthmatics in that they contained a higher proportion of polymorphonuclear (PMN) cells and lower proportion of lymphocytes, predominantly CD3+/CD4+ T helper cells, reflected by a decrease in the chemokine CCL5 in the milk aqueous phase. More PMN and lymphocytes from asthmatic mothers expressed the adhesion molecule CD11b and lymphocytes the IgE receptor CD23, than those from non-asthmatic mothers. Conclusions: Changes to human milk leucocyte prevalence, activation state and cytokines due to maternal asthma may result in changes to immunological priming in the infant. Consequently, the protective effect of long-term breastfeeding may be altered in these mother-infant pairs (AU)


No disponible


Assuntos
Humanos , Feminino , Leucócitos , Leite Humano/citologia , Citocinas/análise , Asma/imunologia , Neutrófilos , Hipersensibilidade/imunologia , Biomarcadores/análise , Fatores de Proteção , Aleitamento Materno , Citometria de Fluxo , Ensaio de Imunoadsorção Enzimática
13.
Rev. esp. cir. oral maxilofac ; 39(2): 91-98, abr.-jun. 2017. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-161183

RESUMO

La regeneración periodontal es la reproducción o reconstitución de una parte perdida o dañada del periodonto con el fin de restaurar su arquitectura y función. En los últimos años se ha puesto de manifiesto el papel clave que juegan las plaquetas en la regeneración tisular, acelerando la cicatrización tanto de tejidos blandos como duros, mediada por la liberación de citocinas y factores de crecimiento durante un tiempo prolongado. La fibrina rica en plaquetas y leucocitos utilizada por primera vez por Choukroun en el 2001 es un concentrado de plaquetas de segunda generación que se obtiene a partir de la propia sangre del paciente, sin el empleo de aditivos, con el fin de conseguir una malla de fibrina que sirva de andamiaje para las sustancias implicadas en la regeneración. El objetivo de este trabajo es el de realizar una revisión y puesta al día en el uso de esta técnica (AU)


Periodontal regeneration is the reproduction or re-enactment of an injured, or lost, part of the periodontium, with the aim of repairing its architecture and main function. The key role of platelets in tissue regeneration has been demonstrated in the last few years. They accelerate healing in both the soft and hard tissues due to the liberation of cytokines and growth factors over a long period. Leucocyte-rich platelet-rich fibrin, used for the first time by Choukroun in 2001, is a second generation platelets extract that is obtained from the patient's own blood, without the need of additives. Its purpose is to attain an autologous fibrin mesh to be used for as a framework for the substances involved in bone regeneration. The purpose of this work is to present a review and an update on the use of this technique (AU)


Assuntos
Humanos , Masculino , Feminino , Regeneração Óssea/fisiologia , Plaquetas , Leucócitos , Periodonto/fisiologia , Tempo de Lise do Coágulo de Fibrina/métodos , Fibrina/uso terapêutico , Periodonto/crescimento & desenvolvimento , Cirurgia Bucal , Materiais Biocompatíveis/uso terapêutico , Substitutos Ósseos/uso terapêutico
14.
Clín. investig. arterioscler. (Ed. impr.) ; 29(2): 60-66, mar.-abr. 2017. tab
Artigo em Inglês | IBECS | ID: ibc-161016

RESUMO

Aims: To test the hypothesis that the pattern of gene expression in circulating leukocytes may differ between vascular compartments, depending on the presence or absence of atherosclerosis, we evaluated the regional vascular differences in patterns of inflammatory cell activation. Methods: Patients (n = 8) with angiographically-established coronary artery disease (CAD+) and 8 without (CAD-) had blood samples taken from a peripheral vein as well as from left and right coronary arteries. Samples were pooled resulting in 4 CAD+ samples versus 4 CAD- samples and hybridised to a Whole Human Genome Microarray 4×44K. Results: CAD- patients had a similar gene expression profile across the different sites. CAD+ patients had statistically significant different gene expression patterns in venous vs. right and left coronary artery compartments. The expression pattern observed in the right coronary was where the most differences in gene expression were observed in CAD+ vs. CAD- patients. Overall, 1964 genes were differentially expressed between CAD+ and CAD−. Of these, 1052 were less expressed in CAD+ and 912 were more expressed in CAD+. Up to 12 of the 20 most differentially expressed genes appeared to reflect different phases of the atherosclerosis process: endothelial dysfunction, lipid accumulation, and smooth muscle cell proliferation. Conclusions: Gene expression of circulating leukocytes differentiates CAD+ from CAD- patients. Gene expression is significantly different between coronary arteries and the systemic circulation in CAD+ patients, but not in CAD- patients. Gene expression is significantly different between CAD+ and CAD- subjects, and appears to reflect the atherosclerosis process. These intra-individual differences may be an additional feature of established coronary artery disease


Objetivo: Para comprobar la hipótesis de que los patrones de expresión génica de leucocitos en circulación pueden ser diferentes entre los compartimentos vasculares dependiendo de la presencia o ausencia de arteriosclerosis, hemos evaluado en distintas regiones vasculares las diferencias entre los patrones de expresión y la activación de células inflamatorias. Métodos: Se extrajeron muestras de sangre de venas periféricas y de las arterias coronarias (derecha e izquierda) de pacientes con (n=8; CAD+) y sin (n=8; CAD−) enfermedad arterial coronaria establecida angiográficamente. Las muestras fueron hibridadas en dos pooles de 4 muestras (CAD+ vs CAD−) mediante el kit Whole Human Genome Microarray 4×44K. Resultados: Los pacientes CAD- tenían un perfil de expresión génica similar entre los distintos compartimentos vasculares. Los pacientes CAD+ tenían patrones de expresión génica significativamente diferentes entre los compartimentos venosos y las arterias coronarias derecha e izquierda. El patrón de expresión observado en la arteria coronaria derecha fue el que presentó más diferencias entre los pacientes CAD+ vs. CAD-. En conjunto, 1.964 genes estaban expresados diferencialmente entre CAD+ y CAD-. De estos, 1.052 estaban menos expresados en CAD+ i 912 estaban más expresados en CAD+. Hasta 12 de los 20 genes más diferencialmente expresados estaban relacionados con las diferentes fases del proceso arteriosclerótico: disfunción endotelial, acumulación lipídica y proliferación de células musculares lisas. Conclusiones: La expresión génica de leucocitos circulantes diferencia pacientes CAD+ de CAD-. La expresión genética es significativamente diferente entre arterias coronarias y circulación sistémica en pacientes CAD+, pero no en pacientes CAD-. Estas diferencias intraindividuales podrían ser una característica adicional en el diagnóstico de la enfermedad arterial coronaria


Assuntos
Humanos , Doença da Artéria Coronariana/fisiopatologia , Leucócitos , RNA/análise , Aterosclerose/fisiopatologia , Expressão Gênica , Endotélio Vascular/fisiopatologia , Lipidoses/fisiopatologia , Miócitos de Músculo Liso
16.
Clin. transl. oncol. (Print) ; 18(3): 251-258, mar. 2016. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-148708

RESUMO

Tumor-associated macrophages (TAMs) are major component of leukocytic infiltrate of tumors and play important roles in progression and regression of tumors. Tumor microenvironment determines the mutual conversion between M1 and M2 macrophages. In many kinds of tumors, M2 type macrophages are of the majority in TAMs and promote tumor progression and metastasis. The dynamic balance and interaction between TAMs and tumor cells have important effects on the occurrence and development of tumor. TAMs in malignant tumors are useful for clinical diagnosis and may provide a novel target for cancer treatment (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Macrófagos/citologia , Fatores de Necrose Tumoral/farmacologia , Leucócitos/citologia , Lipopolissacarídeos/química , Proteínas Proto-Oncogênicas c-sis/administração & dosagem , Linfonodos/metabolismo , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos , Carcinoma Hepatocelular/patologia , Neoplasias Gástricas/tratamento farmacológico , Helicobacter pylori/citologia , Macrófagos/classificação , Fatores de Necrose Tumoral/metabolismo , Leucócitos/química , Lipopolissacarídeos , Proteínas Proto-Oncogênicas c-sis/classificação , Linfonodos/anormalidades , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Carcinoma Hepatocelular/tratamento farmacológico , Neoplasias Gástricas/radioterapia , Helicobacter pylori/metabolismo
17.
Rev. esp. anestesiol. reanim ; 63(2): 78-83, feb. 2016. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-150335

RESUMO

Objetivos. Determinar las características hematológicas y microbiológicas de la sangre recuperada mediante el uso de un recuperador celular con campana centrifugadora pediátrica rígida (100 ml) en cirugía de escoliosis en pediatría y comprobar si se ajusta al estándar esperado en el paciente adulto. Material y método. Estudio de cohorte transversal, descriptivo, sobre 24 unidades consecutivas de sangre recuperada del campo quirúrgico procesadas mediante un recuperador de sangre modelo Haemolite® 2+ (Haemonetics Corp., Braintree, MA, EE. UU.). Se recogieron los datos referentes a edad, peso, abordaje (anterior o posterior) de la cirugía de escoliosis, volumen procesado y volumen de concentrado de hematíes (CH) autógeno recuperado, hemograma y hemocultivo del concentrado obtenido y la incidencia de fiebre tras la reinfusión. Resultados. El volumen procesado fue muy escaso (939 ± 569 ml) con gran variabilidad (coeficiente de variación = 0,6), a diferencia del volumen recuperado 129 ± 50 ml (coeficiente de variación = 0,38). Se estableció correlación estadísticamente significativa entre el volumen procesado y el hematocrito del CH recuperado (Pearson, r = 0,659; p = 0,001) que fue menor del esperado. Los parámetros hematológicos más relevantes de los concentrados recuperados fueron: Hb 11 ± 5,3 g dl−1; HTO: 32,1 ± 15,4%; leucocitos 5,34 ± 4,22 × 103 μl−1; plaquetas 37,88 ± 23,5 × 103 μl−1 (media ± DE). El hemocultivo del CH recuperado fue positivo en 13 casos (54,2%) en los que se aisló Staphylococcus coagulasa (−). Conclusiones. Los recuperadores celulares con campana centrifugadora de volumen fijo (incluso pediátrica) no obtienen la concentración esperada si se procesan bajos volúmenes, por lo que no son la mejor opción en el niño (AU)


Objective. To determine the haematological and microbiological characteristics of blood recovered by using a cell saver with a rigid centrifuge bowl (100 ml) in paediatric scoliosis surgery and to determine whether it conforms to the standard expected in adult patients. Material and methods. A cross-sectional, descriptive cohort study was performed on 24 consecutive red blood cell (RBC) units recovered from the surgical field and processed by a Haemolite® 2+ (Haemonetics Corp., Braintree, MA, EE. UU.) cell saver. Data were collected regarding age, weight, surgical approach (anterior or posterior), processed shed volume and volume of autologous RBC recovered, full blood count, and blood culture obtained from the RBC concentrate, and incidence of fever after reinfusion. Results. The processed shed volume was very low (939 ± 569 ml) with high variability (coefficient of variation = 0.6), unlike the recovered volume 129 ± 50 ml (coefficient of variation = 0.38). A statistically significant correlation between the processed shed volume and recovered RBC concentrate haematocrit was found (Pearson, r=.659, P=.001). Haematological parameters in the recovered concentrate were: Hb 11 ± 5.3 g dl−1; haematocrit: 32.1 ± 15.4% (lower than expected); white cells 5.34 ± 4.22 × 103 ul−1; platelets 37.88 ± 23.5 × 103 ul−1 (mean ± SD). Blood culture was positive in the RBC concentrate recovered in 13 cases (54.2%) in which Staphylococcus coagulase (−) was isolated. Conclusions. Cell salvage machines with rigid centrifuge bowls (including paediatric small volume) do not obtain the expected haematocrit if low volumes are processed, and therefore they are not the best choice in paediatric surgery (AU)


Assuntos
Humanos , Masculino , Feminino , Transfusão de Sangue Autóloga/classificação , Escoliose/metabolismo , Pediatria/educação , Estudos Transversais/métodos , Ortopedia/educação , Eritrócitos/citologia , Hemoglobina A/administração & dosagem , Assepsia/métodos , Leucócitos/citologia , Transfusão de Sangue Autóloga/métodos , Escoliose/patologia , Pediatria/métodos , Estudos Transversais , Ortopedia/organização & administração , Eritrócitos/patologia , Hemoglobina A/metabolismo , Assepsia/normas , Leucócitos/metabolismo
18.
Rev. Rol enferm ; 38(10): 650-656, oct. 2015. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-143468

RESUMO

Introducción. El análisis de orina con tiras reactivas es una prueba muy usada en la clínica. Su uso más habitual es de confirmar o excluir la sospecha de infección de orina, pero también son útiles en la gestión de otras enfermedades. Desarrollo. Las tiras de orina más usadas disponen de 10 reactivos: leucocitos, nitritos, pH, glucosa, proteínas, cuerpos cetónicos, bilirrubina, urobilinógeno, densidad y sangre, para un análisis cualitativo y semicuantitativo de las muestras de orina. Se sumergirá en la muestra de orina y, tras esperar el tiempo indicado en el envase, se podrán interpretar los resultados, siempre teniendo en cuenta que hay un gran número de falsos positivos y negativos y que los resultados están influenciados por múltiples factores. Discusión. El uso más extendido de las tiras de orina es como ayuda diagnóstica ante la sospecha de infección, aunque la clínica nos oriente en gran medida. La probabilidad de tener infección de orina si hay disuria, frecuencia y urgencia miccional, hematuria, dolor suprapúbico y sensibilidad en la pelvis es de un 90 %. Si hay únicamente disuria y urgencia o frecuencia miccional la probabilidad disminuye a un 70-80 %, y si solo hay disuria, a un 25 %. Conclusiones. Las tiras de orina son un método rápido, fácil y barato para el diagnóstico y seguimiento de enfermedades, pero sus resultados son muy heterogéneos y están influenciados por factores externos, por lo que deben interpretarse con cautela. La sensibilidad y especificidad de las tiras de orina es muy variable según los estudios (S 46 %-86 % y E 17 %-93 %). Aunque los mayores valores diagnósticos se producen en las consultas de Atención Primaria, donde se estudia a una población más variada y se hace un mayor uso de las tiras, también es donde se requiere un mayor trabajo de diagnóstico diferencial por los numerosos falsos positivos. Se precisan más estudios con mayores especificaciones de los criterios seguidos y de las poblaciones estudiadas para llegar a determinar la verdadera sensibilidad y especificidad de las tiras de orina (AU)


Introduction. Urinalysis using reactive strip is a commonly used in clinical practice. Although mainly indicated as first step test when a urine infection it suspected, it may also be a helpful tool in the management of a wide range of disorders. Development. Standard urine test strips may comprise of up to 10 different chemical pads or reagents (leukocytes, nitrites, pH, glucose, proteins, ketones, bilirubin, urobilinogen, density and blood) that allow a qualitative and semiquantitative analysis of a urine sample. The test method consists of immersing the strip completely in a well-mixed sample of urine and left to stand for the time necessary for the reactions to occur (which is variable depending on the manufacturer). Finally the colors that appear are compared against a specific chromatic scale provided. Several factors may influence the results causing a significant number of false positives and negatives. Such limitations should always be taken into account when reading the test. Discussion. Despite clinical features lead to the suspicion of an infection, urine test strips is a fast screening test that may reinforce the diagnosis. The combination of dysuria, frequency and emergency, hematuria, pain and sensibility in the pelvis reaches a positive predictive value to identified a urine infection of 90 %. When only dysuria and emergency or high frequency are present, the such probability diminishes to 70-80 %, and, when dysuria is the only symptom, it drops to 25 %. Conclusions. Despite urine test strips is a fast, easy and cheap method for the diagnosis and follow-up of several diseases, results are fairly heterogeneous and may be influenced by external factors. Therefore a cautious interpretation if advised. Sensibility and specificity of urine test strips is widely variable (S 46 %-8 6% and E 17 %-93 %). Although the highest diagnostic values are obtained at primary care centers, where such tests are routinely used in a diverse population, the number of false positives is still high. This issue should be taken into account and a proper differential diagnosis of a positive result is mandatory in all cases (AU)


Assuntos
Feminino , Humanos , Masculino , Urina/microbiologia , Urinálise/métodos , Urinálise/enfermagem , Infecções Urinárias/diagnóstico , Infecções Urinárias/enfermagem , Infecções Urinárias/urina , Fitas Reagentes , Sensibilidade e Especificidade , Atenção Primária à Saúde/métodos , Glucose , Bilirrubina , Cetonas , Concentração de Íons de Hidrogênio , Sangue , Proteínas , Nitritos/isolamento & purificação , Leucócitos/microbiologia , Ácido Ascórbico
19.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 33(7): 458-463, ago.-sept. 2015. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-140509

RESUMO

INTRODUCCIÓN: El diagnóstico de infección por Chlamydia trachomatis es difícil en recién nacidos; sin embargo, este se realiza mediante el cultivo celular o por la detección de anticuerpos IgM anti-C. trachomatis (anti-CT). La detección de ADN de C. trachomatis en leucocitos de sangre mediante reacción en cadena de la polimerasa (PCR) podría ser una mejor herramienta para el diagnóstico de infección por este patógeno. MATERIAL Y MÉTODOS: Se analizaron 44 recién nacidos, todos ellos prematuros y con peso menor de 2.500 g. De cada paciente se obtuvieron muestras de sangre y de lavado nasofaríngeo. El ADN de los leucocitos fue obtenido mediante la técnica de fenol-cloroformo. La detección de C. trachomatis fue llevada a cabo mediante la amplificación del gen ompA utilizando el PCR de punto final. Además, se realiza- ron las pruebas de cultivo celular y la detección de anticuerpos IgM anti-CT mediante la técnica de microinmunofluorescencia. RESULTADOS: Veinte pacientes fueron PCR-positivo (45,5%), y la prueba se asoció significativamente con la presencia de neumonía (RR = 2,28; IC 95%: 1,01-5,17; p = 0,035). El cultivo celular de lavado nasofaríngeo solo fue positivo en 7 muestras y no hubo asociación significativa con algún dato clínico o de laboratorio. El título de anticuerpos anti-CT asociado al PCR-positivo fue 1:32 (RR = 2,74; IC 95%: 1,21-6,23; p = 0,008); sin embargo, este título no se asoció a la presencia de neumonía. CONCLUSIÓN: La detección de ADN en leucocitos de sangre periférica podría ser útil para el diagnóstico de infección por C. trachomatis


INTRODUCTION: Diagnosis of Chlamydia trachomatis infection in newborns is difficult; however, this diagnosis is performed by cell culture or by detection of IgM antibodies against C. trachomatis. Detection of C. trachomatis DNA in peripheral blood leukocytes using polymer chain reaction (PCR) may be a better tool for the diagnosis of infection by this pathogen. MATERIAL AND METHODS: A total of 44 premature newborns, all weighing less than 2500 g, were included in the study. A blood sample and nasopharyngeal lavages were obtained from each newborn. Leukocyte DNA was obtained by phenol-chloroform extraction technique. Detection of C. trachomatis was performed by amplifying the ompA gene using the PCR endpoint. Cell culture tests and the detection of IgM antibodies against C. trachomatis by microimmunofluorescence assay were also performed. RESULTS: Twenty newborns were PCR-positive (45.5%), with this test being significantly associated with the presence of pneumonia (RR = 2.28; 95% CI: 1.01 to 5.17; P = .035). The cell culture of nasopharyngeal lavage was positive in only 7 samples and no significant association was observed with any clinical or laboratory data. The titer of IgM antibodies against C. trachomatis associated with PCR-positive was 1:32 (RR = 2.74; 95% CI: 1.21 to 6.23; P = .008), however this titer was not associated with the presence of pneumonia. CONCLUSION: DNA detection in peripheral blood leukocytes could be useful for diagnosis of C. trachomatis infection


Assuntos
Feminino , Humanos , Recém-Nascido , Masculino , Chlamydia trachomatis/isolamento & purificação , DNA/análise , Leucócitos/microbiologia , Imunoglobulina M , Reação em Cadeia da Polimerase/instrumentação , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/microbiologia , Recém-Nascido Prematuro/sangue , Recém-Nascido de Baixo Peso/sangue , Microscopia de Fluorescência/métodos , Imunofluorescência , Meios de Cultura/isolamento & purificação
20.
Rev. Rol enferm ; 38(7/8): 504-510, jul.-ago. 2015. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-138369

RESUMO

Por estudios realizados en nuestro hospital, se observó que todos los años existía una diferencia considerable entre las infecciones de orina en pacientes sondados del servicio de Neurología con respecto a otras unidades de hospitalización. Esto nos llevó a realizar el presente estudio. Objetivo. Determinar las causas que aumentan las infecciones del tracto urinario (ITU) en el paciente sondado con patología neurológica. Metodología. Estudio observacional descriptivo prospectivo de 50 pacientes con patología neurológica ingresados en la planta de Neurología del Hospital de Cabueñes (Gijón), que precisaron sondaje vesical (SV). El trabajo de campo consistió en recoger una muestra para sedimento y cultivo de orina pre y post-SV, además de anotar las causas del sondaje, duración, edad, sexo y diagnóstico en una hoja de registro. Se descartaron aquellos pacientes sondados de urgencias, los que estaban tomando antibiótico y aquellos cuya patología no era neurológica. Resultados. De 50 pacientes estudiados que precisaron SV, el 40 % fue por retención de orina, un 40 % por orden médica y el 20 % por tratamiento con manitol. El 88 % tuvo un diagnóstico de ictus. Un 12 % ya tenía el cultivo positivo antes de ser portadores de SV. Conclusiones. El ictus es la patología neurológica que más precisa SV. Una de las causas más importantes de aquel es la retención de orina. Algunos de los pacientes sondados ya tenían ITU antes de la colocación de SV (AU)


In previous studies carried out in our hospital (Hospital de Cabueñes, Gijón, Spain) it could be noticed/stated that every year there was a considerable difference between the patients with urine catheterization in the Neurology Service and the patients in the other hospital wards. This encourages us to carry out the following research. Objectives. The purpose of this study is to determine the causes that increase the urinary infections in neurological patients with bladder catheterization compared to catheterized patients with other diseases. Analysis of sediment and urine culture before and after the urinary bladder catheterization. Methods. Retrospective, descriptive and observational study of fifty patients with neurological disorders who were hospitalized in the neurological care unit of Hospital de Cabueñes (Gijón, Spain) and who needed urinary catheterization. The fieldwork consisted of collecting a sample for sediment and culture before and after the urinary catheterization and also of registering the causes of the catheterization, the length of the treatment, age and sex of patients on a medical record sheet. Patients who were catheterized in the Emergency (A&E) unit, those who were taking antibiotics and those who did not suffer a neurological disease did not take part in this study. Results. 20 patients (40 %) out of the 50 who took part in this study and who needed urinary catheterization (UC) suffered from urinary retention, another 20 (40 %) were under medical diagnosis and supervision and the last 10 (20 %) were treated with manitol. 44 patients (88 %) were diagnosed with strokes. 12 % of them had a positive culture prior to UC placement. Conclusions. Patients who suffered stokes have a high risk of urine retention due to their functional disability. Some of them had already the urine values altered, they even had infection prior to the urinary catheter placement (AU)


Assuntos
Feminino , Humanos , Masculino , Infecções Urinárias/enfermagem , Infecções Urinárias/prevenção & controle , Doenças do Sistema Nervoso/complicações , Doenças do Sistema Nervoso/enfermagem , Doenças do Sistema Nervoso/patologia , Infecções Urinárias/reabilitação , Infecções Urinárias/etiologia , Estudos Prospectivos , Manitol/uso terapêutico , Sonda , Acidente Vascular Cerebral/enfermagem , Acidente Vascular Cerebral/prevenção & controle , Nitritos/uso terapêutico , Leucócitos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA