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1.
Allergol. immunopatol ; 47(6): 585-590, nov.-dic. 2019. graf
Artigo em Inglês | IBECS | ID: ibc-186552

RESUMO

Introduction and objectives: Transforming growth factor β1 (TGFβ1) and dysregulated microRNA-21 (miR-21) expression is associated with TGFβ/Smad signaling pathway activation and fibrosis. While calcitriol has been shown to improve airway remodeling in asthmatic mice, its mechanism remains unknown. In this study, the effect of calcitriol on the TGFβ/Smad signaling pathway and miR-21 expression in human bronchial fibroblasts was investigated to explore the mechanism of action of calcitriol and the inhaled glucocorticoid, budesonide, in airway remodeling. Materials and methods: Human bronchial fibroblasts were pretreated with budesonide, calcitriol, or budesonide plus calcitriol, and stimulated with TGFβ1 for 48h. Quantitative real-time PCR was used to determine the expression of miR-21. Western blot was used to determine airway remodeling-related proteins, TGFβ/Smad signaling pathway-related proteins, glucocorticoid receptor, and vitamin D receptor (VDR) expression. Results: Both budesonide and calcitriol down-regulated miR-21 expression in human bronchial fibroblasts, up-regulated Smad7 expression, and inhibited the expression of airway remodeling-related proteins. Both budesonide and calcitriol up-regulated the low expression of VDR induced by TGFβ1 in human bronchial fibroblasts. The expression of VDR in the combined treatment group (budesonide plus calcitriol) was significantly higher than that in the calcitriol treatment group. The expression of collagen type I in the combined treatment group was significantly lower than that in the calcitriol treatment group. Conclusions: Calcitriol can up-regulate the expression of VDR in human bronchial fibroblasts and exert an anti-airway remodeling effect. Budesonide can up-regulate the expression of VDR in human bronchial fibroblasts and enhance the inhibitory effect of calcitriol on airway remodeling


No disponible


Assuntos
Animais , Camundongos , Budesonida/uso terapêutico , Vitamina D/uso terapêutico , Fibroblastos/imunologia , Calcitriol/uso terapêutico , MicroRNAs/imunologia , Asma/imunologia , Fator de Crescimento Transformador beta1/imunologia , Western Blotting , Fator de Crescimento Transformador beta1/metabolismo , Análise de Variância
2.
Eur. j. anat ; 23(6): 479-481, nov. 2019. ilus
Artigo em Inglês | IBECS | ID: ibc-185092

RESUMO

The claim made in this publication of the existence of a hitherto unknown interstitial space is based on studies with sample-based confocal laser endo-microscopy (pCLM). Due to postings on various web portals (New Cellvizio, EurekAlert, Google Scholar,...) the alleged discovery has found great resonance. Nevertheless, there are several critical issues in this publication, the most important being that this is not the discovery of an "unrecognized" interstitium as it has, in fact, been known for a long time


No disponible


Assuntos
Humanos , Fáscia/anatomia & histologia , Fáscia/ultraestrutura , Pele/ultraestrutura , Espaço Extracelular , Fibroblastos/ultraestrutura , Pele/anatomia & histologia , Derme/anatomia & histologia , Derme/ultraestrutura , Imageamento Tridimensional
3.
Rev. esp. patol. torac ; 31(3): 158-173, oct. 2019. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-187171

RESUMO

Objetivo: investigar la posible degradación de stents metálicos tras co-cultivo con células respiratorias in vitro. Método: durante 21 días se han co-cultivado con la línea CRL-4011 (epitelial) y MRC-5 (fibroblastos) tres tipos de stents: Wallstent(R) (aleación de cobalto-cromo-níquel y molibdeno), Zilver PTX(R) y Zilver Flex(R) (nitinol, aleación de níquel-titanio, con y sin liberación de paclitaxel, respectivamente). Las mismas células sin stent sirvieron como control. Los sobrenadantes de los cultivos se recogieron los días 3, 9, 15 y 21, se alicuotaron y almacenaron a -800 C. Mediante espectrometría de masas (ICP/ MS) se investigaron los niveles de titanio, cromo, níquel, cobalto y molibdeno en los sobrenadantes, y también se han analizado los niveles de esos mismos elementos en el medio de cultivo original (antes de añadirlo a los cultivos celulares). Resultados: en todos los experimentos se encontraron mayores niveles de elementos metálicos en los sobrenadantes recogidos en el tercer día de cultivo, tanto de células epiteliales como de fibroblastos, con diferencias estadísticamente significativas (p<0,002). Los sobrenadantes de los cultivos de células epiteliales con Wallstent mostraron los niveles más altos de níquel y cobalto respecto a los controles (p = 0,001), y los niveles de titanio fueron más altos en los cultivos de Zilver Flex y PTX, constituidos por una aleación de níquel y titanio (p <0,001). Conclusiones: hemos detectado una rápida liberación en el sobrenadante de todos los cultivos de los elementos constitutivos de los tres stents que incluimos en los experimentos, y con niveles muy superiores a los cultivos controles


Objective: To investigate the possible degradation of metal stents after co-culture with respiratory cells in vitro. Methods: Three types of stents were co-cultured with the CRL-4011 line (epithelial) and the MRC-5 line (fibroblasts): Wallstent(R) (cobalt-chromium-nickelmolybdenum alloy), Zilver PTX(R) and Zilver Flex(R) (nitinol, nickel-titanium alloy, with and without paclitaxel release, respectively). The same stentless cells served as the control group. Culture supernatants were collected on days 3, 9, 15 and 21, aliquoted and stored at -80 ºC. The levels of titanium, chromium, nickel, cobalt and molybdenum in the supernatants were studied using inductively coupled plasma mass spectrometry (ICP-MS), and the levels of the same elements were also analyzed in the original culture medium (before adding them to the cell cultures). Results: In all experiments, higher levels of metal elements were found in the supernatants collected on the third day of culture, for both epithelial cells and fibroblasts, with statistically significant differences (p < 0.002). The supernatants of epithelial cell cultures with Wallstent showed the highest levels of nickel and cobalt in comparison to controls (p = 0.001), and titanium levels were higher in Zilver Flex and Zilver PTX cultures, consisting of a nickeltitanium alloy (p < 0.001). Conclusion: We have detected a rapid release in the supernatant of all the cultures of the constituent elements of the three stents that we included in the experiments, and with levels much higher than those of the control cultures


Assuntos
Stents Metálicos Autoexpansíveis , Técnicas In Vitro/métodos , Células Epiteliais/patologia , Fibroblastos/citologia , Meios de Cultura , Stents/classificação , Espectrometria de Massas/métodos , Técnicas de Cultura , Níquel/química , Cobalto/química , Titânio/química
4.
Rev. senol. patol. mamar. (Ed. impr.) ; 32(1): 17-25, ene.-mar. 2019. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-187029

RESUMO

A pesar de los avances conseguidos en el tratamiento del cáncer de mama, todavía sigue siendo una causa importante de mortalidad en las mujeres. Por tanto, resulta necesario plantear nuevos enfoques de la fisiopatología de la enfermedad que contribuyan a realizar una mejor evaluación pronóstica, y a la mejora de las estrategias terapéuticas. Para ello, deberíamos considerar que el cáncer no es solo una transformación maligna de las células epiteliales y su progresión meramente autónoma; sino que, hoy en día, existen datos que apoyan el concepto del cáncer como un ecosistema basado en una sociología de diferentes tipos celulares, con sus interacciones complejas. Entre los diversos tipos de células que conforman el estroma tumoral, y que tienen un papel relevante en la progresión del cáncer de mama, se encuentran los fibroblastos asociados al cáncer, las células inflamatorias y las células endoteliales. Existen diferentes factores moleculares expresados por esas células que se asocian con el desarrollo de metástasis, tales como las metaloproteasas de matriz y sus inhibidores tisulares, citoquinas o receptores tipo toll. En base a la expresión de todos ellos, aquí proponemos 2 fenotipos de estroma del cáncer de mama con influencias marcadamente diferentes sobre el pronóstico de las pacientes. También analizamos los mecanismos involucrados en la interrelación tumor-estroma que pueden llevarnos a mejorar las estrategias terapéuticas en el cáncer de mama


Despite advances in the treatment of breast cancer, it remains an important cause of mortality in women. Therefore, it is necessary to propose new approaches to the pathophysiology of the disease that could help to improve prognostic evaluation and therapeutic strategies. To do this, we should consider that cancer is not only a malignant transformation of the epithelial cells or their purely autonomous growth; nowadays, there are data that support the concept of cancer as a system based on a sociology of different cell types, with complex interactions. Among the various types of cells that make up the tumour stroma and which play an important role in the progression of breast cancer are cancer-associated fibroblasts, inflammatory cells and endothelial cells. Several molecular factors expressed by these cells are associated with the development of metastases, such as matrix metalloproteases and their tissue inhibitors, cytokines or toll-like receptors. Based on the expression of all of these factors, here we propose two types of stroma from breast cancer that display markedly different influences on patient prognosis. We also analyse mechanisms involved in the tumour-stroma interrrelationship that could help to improve therapeutic strategies in breast cancer


Assuntos
Humanos , Células Estromais/patologia , Neoplasias da Mama/patologia , Fibroblastos/patologia , Células Endoteliais/patologia , Mediadores da Inflamação/análise , Prognóstico , Receptores Toll-Like/análise , Junções Intercelulares/patologia
5.
Av. odontoestomatol ; 34(6): 299-309, nov.-dic. 2018. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-182274

RESUMO

Introducción: Los fibroblastos gingivales (FGs) son células responsables del mantenimiento de la homeostasis, cicatrización y tolerancia inmunitaria del tejido conjuntivo gingival. Por sus características fisiológicas, los FGs pueden ser un candidato en la terapia celular. Sin embargo, la conservación de su fenotipo en cultivo celulares requiere de condiciones estrictamente definidas. Una de ellas es la concentración de Suero Fetal Bovino (SFB). Nuestro objetivo es evaluar el efecto concentración-dependiente de la suplementación de SFB en el medio de cultivo sobre el comportamiento, crecimiento, proliferación y supervivencia de los fibroblastos gingivales humanos. Materiales y métodos: FGs fueron cultivados con DMEM (Dulbecco's modification of Eagle médium) y concentraciones de 0%, 0.5% y 10% de SFB durante tres semanas. Análisis morfológico, de proliferación e inmunohistoquímicos fueron llevados a cabo en el presente trabajo. Se llevó a cabo un análisis factorial de varianza (ANOVA) por medio el software R Project. Los resultados fueron considerados significativos con un valor de p<0,05. Resultados y Discusión: Los FGs cultivados con 10% de SFB alcanzaron un desarrollo morfológico más notorio y en menor tiempo comparados con los FGs cultivados a 0.5% y 0% de SFB. La efectividad de la concentración del SFB 0,5% fue mucho más alta en relación a la concentración del SFB 10%. La inmunodetención de la actina, vimentina y fibronectina fueron más notorias en los FGs tratados con 10% y 0.5% de SFB. Este estudio concluye que los FGs humanos presentan una mejor capacidad de supervivencia, desarrollo y proliferación cuando son cultivados en presencia de SFB


Introduction: Gingival fibroblasts (FGs) are cells responsible for the maintenance of homeostasis, healing and immune tolerance of the gingival connective tissue. Due to their physiological characteristics, FGs can be a candidate in cell therapy. However, the conservation of their phenotype in cell culture requires strictly defined conditions. One of them is the concentration of Bovine Fetal Serum (FBS). Our aim is to evaluate the concentration-dependent effect of SFB supplementation in the culture medium on the behavior, growth, proliferation and survival of human gingival fibroblasts. Materials and methods: FGs were cultured with DMEM (Dulbecco's modification of Eagle medium) and concentrations of 0%, 0.5% and 10% of SFB for three weeks. Morphological, proliferation and immunohistochemical analyzes were carried out in the present work. A factorial analysis of variance (ANOVA) was carried out using the R Project software. The results were considered significant with a value of p <0.05. Results and discussion: The FGs grown with 10% FBS reached a more noticeable morphological development and in a shorter time compared to the FGs grown at 0.5% and 0% FBS. The effectiveness of the concentration of the SFB 0.5% was much higher in relation to the concentration of the SFB 10%. The immunodetention of actin, vimentin and fibronectin were more evident in the FGs treated with 10% and 0.5% FBS. This study concludes that human FGs have a better capacity for survival, development and proliferation when they are grown in the presence of FBS


Assuntos
Humanos , Fibroblastos/citologia , Fibroblastos/efeitos da radiação , Gengiva/citologia , Proliferação de Células , Fibroblastos/efeitos dos fármacos , Células Cultivadas , Tecido Conjuntivo/fisiologia , Análise de Variância , Imuno-Histoquímica , Fotomicrografia , Citoesqueleto
6.
Rev. esp. patol. torac ; 30(2): 130-135, jun. 2018. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-180249

RESUMO

En el tejido pulmonar de modelos murinos, la angiotensina II induce la proliferación de fibroblastos, su diferenciación a miofibroblastos y la producción de procolágeno tras su unión al receptor I de la angiotensina. Hemos estudiado el comportamiento de fibroblastos pulmonares humanos procedentes de una línea celular comercial tras la estimulación con TGF-β1. Hemos observado que estos fibroblastos, cuando son estimulados, aumentan la expresión de bFGF, colágeno y α-SMA. Tras el bloqueo del receptor de Angiotensina II con Losartan a una concentración de 10 µM y la estimulación con TGF- β1, se produce una disminución, tanto de los niveles de bFGF como de la concentración de colágeno, sin que llegue a alcanzar la significación estadística con respecto a las células no tratadas. En cuanto a la expresión de α-SMA como marcador de transformación a miofibroblastos, no había diferencias entre las células tratadas con TGF-β1 y TGF-β1 más losartán


In murine model lung tissue, angiotensin II induces the proliferation of fibroblasts, their distinction from myofibroblasts and procollagen production after its binding with the type 1 receptor. We have studied the behavior of human lung fibroblasts from a commercial cell line after stimulation with TGF-β1. We observed that those fibroblasts, when stimulated, increased bFGF, collagen and α-SMA expression. After blocking the angiotensin II receptor with losartan at a concentration of 10 µM and stimulation with TGF- β1, there was a decrease in both bFGF levels and collagen concentration, without reaching statistical significance with regard to untreated cells. With regard to α-SMA expression as an indicator of transformation to myofibroblasts, there were no differences between cells treated with TGF-β1 and TGF-β1 with losartan


Assuntos
Humanos , Fibroblastos/citologia , Fibrose Pulmonar Idiopática/fisiopatologia , Angiotensina II , Modelos Animais , Fator de Crescimento Transformador beta
7.
Arch. med. deporte ; 35(183): 50-55, ene.-feb. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-177444

RESUMO

Introduction: Therapeutic ultrasound is one of the most used physical resources in the area of physiotherapy for the treatment of injuries. However, the multiplicity of dosimetry used in clinical practice points to its indiscriminate use for pathologies that surround skeletal muscle and expresses the limitation of the available literature on the ideal dosimetric standardization to the tissue restoration, mechanism of action and its real effects on the treatment in question. Objective: The objective of this study was to promote a systematic review about the different effects and the dosimetric parameters of therapeutic ultrasonic irradiation on the process of restoration of fibroblast cells in vitro. Methods: To select the articles, three electronic data banks were consulted, with publication from January 2000 to September 2016. The studies were tracked by three freestanding reviewers, according to inclusion and exclusion criteria. Results: 669 articles were selected and after the application of inclusion and exclusion criteria, 647 were excluded. Among the exclusions reasons there are: the utilization of another physical method, exclusive focus on another type of cell line, other experimental models or the use of another language, reaching at the end 22 studies directed to qualitative analysis. Conclusion: The results of this study showed that the scientific basis is not enough to stablish real effects and dosimetric parameters of therapeutic ultrasonic on the process of restoration of fibroblast cells in vitro, due to the lack of generalization and conflict of found results


Introducción: El ultrasonido terapéutico es uno de los recursos físicos más utilizados en el área de fisioterapia para el tratamiento de lesiones. Sin embargo, la gran cantidad de dosimetrías utilizadas en la práctica clínica muestra su uso indiscriminado para patologías que circundan el músculo esquelético y además expresa la limitación de la literatura sobre la estandarización dosimétrica ideal para la restauración del tejido, mecanismo de acción y sus efectos reales sobre el tratamiento en cuestión. Objetivos: El objetivo de este estudio fue realizar una revisión sistemática sobre los diferentes efectos y parámetros dosimétricos de la irradiación ultrasónica terapéutica en el proceso de reparación de células fibroblásticas in vitro. Material y método: Para la selección de los artículos fueron consultadas tres bases de datos para buscar publicaciones entre enero de 2000 y septiembre de 2016. La búsqueda de trabajos se realizó por tres revisores independientes, conforme a los criterios de inclusión y exclusión. Resultados: Se seleccionaron 669 artículos y tras la aplicación de los criterios de inclusión, se excluyeron 647 estudios. Entre los motivos de exclusión están la utilización de otro medio físico, enfoque exclusivo de otro tipo de línea celular, otros modelos experimentales o el uso de otro idioma, quedando 22 estudios para el análisis cualitativo. Conclusión: Los hallazgos de este estudio mostraron que la base científica todavía es insuficiente para el establecimiento de los efectos reales y parámetros dosimétricos de la irradiación ultrasónica terapéutica en el proceso de reparación de células fibroblásticas in vitro, por la falta de generalización y conflicto de los resultados encontrados


Assuntos
Dosimetria in Vivo/métodos , Fibroblastos/efeitos da radiação , Sobrevivência Celular/efeitos da radiação , Terapia por Ultrassom/métodos , 25783 , Fibroblastos/citologia , Doses de Radiação , Terapia com Luz de Baixa Intensidade/métodos
8.
Clín. investig. arterioscler. (Ed. impr.) ; 29(6): 231-238, nov.-dic. 2017. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-169580

RESUMO

Introduction: Previous studies show that mercury exposure increases cardiovascular risk, although the underlying cellular mechanisms have still not been fully studied. The aim of this project is to study, in vascular fibroblasts (VF), the effect of HgCl2 exposure on the expression of enzymes involved in the synthesis of prostanoids and reactive oxygen species (ROS). These molecules have been shown to participate in the inflammatory response associated with cardiovascular diseases. Material and methods: Adventitial VF cultures of Sprague-Dawley rat aortas, shown to be α-actin negative by immunofluorescence, were exposed to HgCl2 (0.05-5μg/mL) for 48h. mRNA and protein levels of cyclooxygenase-2 (COX-2), microsomal prostaglandin E synthase 1 (mPGES-1), thromboxane A2 synthase (TXAS), NADPH oxidase 1 (NOX-1), and 4 (NOX-4) where analyzed using qRT-PCR and western blot, respectively. NOX activity was determined by chemiluminescence. Results: HgCl2 exposure increased COX-2, mPGES-1, TXAS, and NOX-1 expression and NOX activity, and decreased NOX-4 expression. The increase in NOX-1 and COX-2 expression was abolished by the treatment with inhibitors of COX-2 (10μM celecoxib) and NOX (300μM apocynin, 0.5μM ML-171). Conclusions: 1) HgCl2 increases the expression of pro-inflammatory enzymes involved in ROS and prostanoid synthesis in VF. 2) There is a reciprocal regulation between COX-2 and NOX-1 pathways. 3) These effects can contribute to explain the increase in cardiovascular risk associated to mercury (AU)


Introducción: Estudios previos muestran que la exposición a mercurio aumenta el riesgo cardiovascular, sin embargo, los mecanismos celulares subyacentes no han sido esclarecidos completamente. Nuestro objetivo es estudiar el efecto de la exposición a HgCl2 sobre la expresión de enzimas involucradas en la síntesis de prostanoides y especies reactivas de oxígeno (ROS) en fibroblastos vasculares (FV). Se ha demostrado la participación de estas moléculas en la respuesta inflamatoria asociada a enfermedades cardiovasculares. Métodos: FV de la adventicia de aorta de ratas Sprague-Dawley, caracterizados por inmunofluorescencia como negativos para α-actina, fueron estimulados con HgCl2 (0,05-5μg/ml) durante 48 horas. Se analizaron los niveles de ciclooxigenasa-2 (COX-2), prostaglandina E sintasa 1 microsomal (mPGES-1), tromboxano A2 sintasa (TXAS), NADPH oxidasa 1 (NOX-1) y 4 (NOX-4) mediante qRT-PCR y western blot, respectivamente. La actividad de NOX se determinó mediante quimioluminiscencia. Resultados: La exposición a HgCl2 aumentó la expresión de COX-2, mPGES-1, TXAS y NOX-1, disminuyendo la expresión de NOX-4. El tratamiento con inhibidores de COX-2 (10μM celecoxib) y NOX (300μM apocynin, 0.5μM ML-171) abolió el aumento de la expresión de NOX-1 y COX-2, respectivamente. Conclusiones: 1) HgCl2 aumenta la expresión de enzimas proinflamatorias implicadas en la síntesis de ROS y prostanoides en FV. 2) Hay una regulación recíproca entre las vías de COX-2 y NOX-1. 3) Estos efectos pueden contribuir a explicar el aumento del riesgo cardiovascular asociado a la exposición al mercurio (AU)


Assuntos
Animais , Ratos , Doenças Cardiovasculares/induzido quimicamente , Doenças Cardiovasculares/veterinária , Fibroblastos , Estresse Oxidativo , Ratos Sprague-Dawley , Imunofluorescência , Inibidores de Ciclo-Oxigenase 2/uso terapêutico , Western Blotting
10.
Actas urol. esp ; 41(6): 376-382, jul.-ago. 2017. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-164453

RESUMO

Objetivo: Analizar el comportamiento de la metaloprotesa 11 (MMP11) en fibroblastos cultivados procedentes de tumores prostáticos humanos con diferentes características clinicopatológicas. Material y métodos: Para este estudio se analizaron muestras de biopsias de próstata obtenidas por vía transrectal de tumores con diferentes características, tratados o no con privación androgénica (PA). Tras la optimización del método de cultivo, se aislaron y cultivaron los fibroblastos para realizar el estudio (PCR) del ARNm de MMP11. Resultados: Se estudiaron finalmente 37 casos: 5 muestras de hiperplasia benigna de próstata, 14 casos con neoplasias localizadas (7 de alto riesgo según la clasificación de D’Amico), 5 con tumores con metástasis óseas y 13 tratados con PA, de los que 6 cumplían los requisitos para ser definidos como resistentes a la castración. En los tumores sin PA, la expresión de MMP11 fue significativamente mayor (p = 0,001) en los fibroblastos de tumores de grados más altos. Se encontró una correlación significativa (p = 0,001) entre PSA y expresión de MMP11 fibroblástica y un aumento significativo de la expresión de MMP11 en los tumores metastásicos. En los tumores con PA se objetivó una expresión significativamente mayor de MMP11 en pacientes resistentes a la castración que en los sensibles a esta (p = 0,003). Conclusión: En tumores de próstata avanzados o en fases de mayor agresividad tumoral, la producción de MMP11 por los fibroblastos resulta significativamente mayor que en tumores no metastásicos o en fases de sensibilidad a la PA


Objective: To analyze the expression of metalloprotein 11 (MMP11) in cultured fibroblasts obtained from human prostate tumors with different clinical and pathological characteristics. Material and methods: For this study we analyzed samples of transrectal prostate biopsies from tumors with different characteristics, treated with or whithout androgen deprivation (AD). After optimization of the culture method, fibroblasts were isolated and cultured to perform the study (PCR) of MMP11 mRNA. Results: Finally, 37 cases were studied: 5 samples of benign prostatic hyperplasia, 14 cases with localized neoplasms (7 high-risk according to the D’Amico classification), 5 with metastasic tumors (bone metastases), and 13 treated with AD therapy, of which 6 fulfilled the requirements to be defined as resistant to castration. In tumors without AD therapy, MMP11 expression was significantly higher (P= .001) in fibroblasts of higher grade tumors. A significant (P= .001) correlation was found between PSA and expression of MMP11 in fibroblast s and a significant increase of MMP11 expression in metastatic tumors. In tumors with AD therapy, a significantly greater expression of MMP11 was observed in resistant to castration patients than in those sensitive to castration (P= .003). Conclusion: In advanced prostate tumors or in stages of increased tumor aggressiveness, the production of MMP11 by fibroblasts is significantly greater than in non-metastatic tumors or in AD sensitive tumors


Assuntos
Humanos , Masculino , Neoplasias da Próstata/patologia , Metaloproteinase 11 da Matriz/análise , Neoplasias de Próstata Resistentes à Castração/patologia , Biomarcadores Tumorais/análise , Estadiamento de Neoplasias , Fibroblastos/ultraestrutura , Células Estromais/patologia
11.
Rev. esp. cir. oral maxilofac ; 39(2): 99-104, abr.-jun. 2017. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-161184

RESUMO

La fascitis nodular es una proliferación benigna de fibroblastos y miofibroblastos, previamente descrita como de naturaleza reactiva. Resulta extremadamente rara la presentación en la cavidad bucal. La ubicación predilecta es la mucosa yugal. Clínicamente se observa un tumor de rápido y brusco crecimiento, bordes bien circunscritos, consistencia firme a la palpación, coloración similar a la mucosa normal e indolora. El tratamiento definitivo consiste en la escisión completa de la lesión; las recidivas son excepcionales. Se presenta un caso clínico en una mujer de 23 años de edad con un tumor en encía inferior, de 2 meses de evolución. Se realizó la escisión quirúrgica con diagnóstico de fascitis nodular sin que se observara recurrencia a los 18 meses. Además, se revisan los casos publicados de fascitis nodular de presentación intraoral en los últimos 20 años (AU)


Nodular fasciitis is a benign proliferation of fibroblasts and myofibrobasts, previously described as one of reactive nature. Intraoral presentation is very rare. Clinically, lesions are described as rapid growth masses, well-circumscribed, of elastic consistency and asymptomatic. Treatment of choice consists in complete excision, while recurrences are exceptional. We describe a case in a 23 year-old woman; with an inferior gum tumor which had grown in 2 months. A complete excision was performed arriving to a diagnosis of nodular fasciitis; with an 18 month follow-up without recurrence. Moreover, a review of oral nodular fasciitis of the cases published over the last 20 years is done (AU)


Assuntos
Humanos , Feminino , Recém-Nascido , Criança , Adulto , Pessoa de Meia-Idade , Fasciite/diagnóstico , Fibroma/diagnóstico , Fibroma/cirurgia , Boca/cirurgia , Imuno-Histoquímica , Fibroblastos/citologia , Biópsia/métodos
12.
J. physiol. biochem ; 73(1): 59-65, feb. 2017. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-168393

RESUMO

Human diploid fibroblasts (HDFs) proliferation in culture has been used as a model of aging at the cellular level. Growth arrest is one of the most important mechanisms responsible for replicative senescence. Recent researches have been focusing on the function of vitamin E in modulating cellular signaling and gene expression. Therefore, the aim of this study was to elucidate the effect of palm γ-tocotrienol (vitamin E) in modulating cellular aging through p16INK4a pathway in HDF cells. Primary culture of senescent HDFs was incubated with 70 μM of palm γ-tocotrienol for 24 hours. Silencing of p16INK4a was carried out by siRNA transfection. RNA was extracted from the different treatment groups and gene expression analysis was carried out by real-time reverse transcription polymerase chain reaction. Proteins that were regulated by p16INK4a were determined by western blot technique. The finding of this study showed that p16INK4a mRNA was overexpressed in senescent HDFs, and hypophosphorylated-pRb and cyclin D1 protein expressions were increased (p < 0.05). However, downregulation of p16INK4a and hypophosphorylated-pRb and cyclin D1 protein expressions (p < 0.05) by γ-tocotrienol led to modulation of the cell cycle regulation during cellular aging. In conclusion, senescent HDFs showed change in biological process specifically in cell cycle regulation with elevated expression of genes and proteins which may contribute to cell cycle arrest. Palm γ-tocotrienol may delay cellular senescence of HDFs by regulating cell cycle through downregulation of p16INK4a and hypophosphorylated-pRb and cyclin D1 protein expressions (AU)


No disponible


Assuntos
Humanos , Masculino , Criança , Senescência Celular , Ciclina D1/antagonistas & inibidores , Regulação para Baixo , Cromanos/metabolismo , Fibroblastos/metabolismo , Vitamina E/análogos & derivados , Fenômenos Fisiológicos Celulares , Células Cultivadas , Inibidor p16 de Quinase Dependente de Ciclina/antagonistas & inibidores , Proteínas de Ligação a Retinoblastoma/antagonistas & inibidores , Ubiquitina-Proteína Ligases/antagonistas & inibidores
14.
Clin. transl. oncol. (Print) ; 18(9): 872-877, sept. 2016. graf
Artigo em Inglês | IBECS | ID: ibc-155500

RESUMO

The transcription factor Snail1 leads to the epithelial-mesenchymal transition by repressing the adherent and tight junctions in epithelial cells. This process is related to an increase of cell migratory and mesenchymal properties during both embryonic development and tumor progression. Although Snail1 expression is very limited in adult animals, emerging evidence has placed Snail at the forefront of medical science. As a transcriptional repressor, Snail1 confers cancer stem cell-like traits on tumor cells and promotes drug resistance, tumor recurrence and metastasis. In this review, we summarize recent reports that suggest the pro-tumorigenic roles of Snail1 expression in tumor stroma. The crosstalk between tumor and stromal cells mediated by Snail1 regulates paracrine communication, pro-tumorigenic abilities of cancer cells, extracellular matrix characteristics and mesenchymal differentiation in cancer stem cells and cancer-associated fibroblasts. Therefore, understanding the regulation and functional roles of Snail1 in the tumor microenvironment will provide us with new therapies for treating metastatic disease (AU)


No disponible


Assuntos
Humanos , Tumores do Estroma Gonadal e dos Cordões Sexuais/genética , Células-Tronco Mesenquimais/patologia , Comunicação Parácrina/genética , Fibroblastos/patologia , Matriz Extracelular/patologia , Células-Tronco/patologia
15.
Actas dermo-sifiliogr. (Ed. impr.) ; 106(9): e45-e48, nov. 2015. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-148731

RESUMO

El tricoblastoma es una neoplasia cutánea benigna, con un aspecto clínico e histológico similar al carcinoma basocelular nodular. Comunicamos los hallazgos dermatoscópicos de 2 casos de tricoblastomas localizados en la cara. Un caso presentaba telangiectasias muy finas y cortas, poco ramificadas, sobre un fondo blanquecino perlado. El otro caso mostraba vasos cortos y poco ramificados dispuestos en corona, con estrías blancas y puntos similares a quistes de milium. Aunque las telangiectasias finas y poco ramificadas no son un criterio específico del tricoblastoma, pueden resultar útiles en el diagnóstico diferencial frente al carcinoma basocelular nodular (AU)


Trichoblastoma is a benign cutaneous neoplasm that is clinically and histologically similar to basal cell carcinoma. We report the dermoscopic features seen in 2 cases of facial trichoblastoma. One case presented with very short, delicate, scarcely branching telangiectases against a pearly white background. In the second case, the veins were also short and scarcely branching, but they were arranged in a crown pattern, with white striae and milia-like cysts. Although dermoscopic evidence of fine, scarcely branching telangiectases is not specific to a diagnosis of trichoblastoma, these features may be useful for differentiating this neoplasm from nodular basal cell carcinoma (AU)


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma Basocelular/complicações , Carcinoma Basocelular/diagnóstico , Carcinoma Basocelular/terapia , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/terapia , Carcinoma Basocelular , Diagnóstico Diferencial , Derme/patologia , Derme , Fibroblastos
16.
Nutr. hosp ; 31(6): 2747-2751, jun. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-142265

RESUMO

The administration of different polyphenols protects against increased body weight and fat accumulation. The aim of the study was to determine the anti-adipogenic activity of an olive-seed polyphenolic extract, by means of mouse fibroblast cell line 3T3-L1 adipocyte differentiation. Material and methods: cells were incubated and differentiated (6000 cells/cup) in the presence of olive-seed extract at 10 and 50 mg/l biosecure concentrations of polyphenols, and with no extract in the control sample. After 5 to 7 days mature adipocytes are formed. The fat clusters are quantified by means of red-oil staining, 490 nm absorbance, and the expression of the leptin and PPARg genes, and then compared to the values obtained in the cultures before and after adipocyte differentiation. Results: the control samples, with no extract, presented an accumulation of fat of 100%. By contrast, the addition of 50 mg/l of olive-seed extract polyphenols resulted in a 50% accumulation of fat, similar to that of the non-differentiated cells. A 10 mg/l extract concentration had no effect. Anti-adipogenic activity is thus confirmed, as the expression of the PPARg and leptin genes is reduced in adipocyte differentiation in the presence of extract at 50 mg/l. In conclusion, both the formation of fatty substances characteristic of adipogenesis, and the expression of the adipogenic PPARg and leptin genes are found to be inhibited by the prior addition of olive-seed extract polyphenols at a 50 mg/l concentration (AU)


La administración de diferentes polifenoles protege contra el incremento de peso y la acumulación de grasa. Objetivo: comprobar la actividad anti-adipogénica de un extracto polifenólico de huesos de aceituna, utilizando la diferenciación a adipocitos de la línea celular 3T3-L1 de fibroblastos de ratón. Material y métodos: se cultivan y diferencian las células (6.000 células/pocillo) en presencia del extracto de huesos de aceitunas a 10 y 50 mg/l de polifenoles, concentraciones bioseguras, y sin extracto como control. A los 5-7 días se forman los adipocitos maduros. Se cuantifican los cúmulos de grasa formados mediante tinción con OilRed y medida de la absorbancia a 490 nm y la expresión de los genes de leptina y PPARg, relacionándolos con los valores en los cultivos antes y después de diferenciarse a adipocitos. Resultados: las muestras control, sin extracto, se consideran el 100% de acumulación de grasas. En contraste, la adición de 50 mg/l de extracto de polifenoles de huesos de aceituna muestra un cúmulo de grasa de alrededor del 50%, semejante a las células no diferenciadas. Con 10 mg/l de extracto no se muestra efecto. Se confirma la actividad antiadipogénica, observándose disminución en la expresión de los genes PPARg y de leptina en la diferenciación a adipocitos en presencia del extracto a 50 mg/l. En conclusión, la formación de los cuerpos grasos característicos de la adipogénesis queda inhibida previa adición de 50 mg/l de polifenoles de extracto de huesos de aceituna, así como la expresión de los genes adipogénicos PPARg y de leptina (AU)


Assuntos
Animais , Ratos , Interleucina-11/farmacocinética , Adipogenia , Extratos Vegetais/farmacocinética , Polifenóis/farmacocinética , Fibroblastos , Olea/química , Leptina/farmacocinética , Técnicas In Vitro/métodos
17.
Med. oral patol. oral cir. bucal (Internet) ; 20(2): 180-187, mar. 2015. ilus
Artigo em Inglês | IBECS | ID: ibc-134130

RESUMO

Objectives: Abnormal cellular immune response has been considered to be responsible for oral lesions in recurrent aphthous stomatitis. Zinc has been known to be an essential nutrient metal that is necessary for a broad range of biological activities including antioxidant, immune mediator, and anti-inflammatory drugs in oral mucosal disease. The objective of this study was to investigate the effects of zinc in a phorbol-12-myristate-13-acetate (PMA)-treated inflammatory model on human gingival fibroblast cells (hGFs).Study Design: Cells were pre-treated with zinc chloride, followed by PMA in hGFs. The effects were assessed on cell viability, cyclooxygenease-1,2(COX-1/2) protein expression, PGE2 release, ROS production and cytokine release, Results: The effects were assessed on cell viability, COX1/2 protein expression, PGE2 release, ROS production, cytokine release. The results showed that, in the presence of PMA, zinc treatment leads to reduce the production of ROS, which results in decrease of COX-2 expression and PGE2 release. Conclusions: Thus, we suggest that zinc treatment leads to the mitigation of oral inflammation and may prove to be an alternative treatment for recurrent aphthous stomatitis (AU)


Assuntos
Humanos , Compostos de Zinco/farmacocinética , Fibroblastos , Estomatite Aftosa/tratamento farmacológico , Anti-Inflamatórios/farmacocinética , Inflamação/fisiopatologia , Ciclo-Oxigenase 2/análise , Mediadores da Inflamação/análise
18.
Clin. transl. oncol. (Print) ; 17(1): 1-10, ene. 2015. ilus
Artigo em Inglês | IBECS | ID: ibc-131899

RESUMO

The purpose of translation is the development of effective medicinal products based on validated science. A parallel objective is to obtain marketing authorization for the translated product. Unfortunately, in solid cancer, these two objectives are not mutually consistent as evidenced by the contrast between major advances in science and the continuing dismal record of pharmaceutical productivity. If the problem is unrelated to science, then the process of translation may require a closer examination, namely, the criteria for regulatory approval. This realization is important because, in this context, the objective of translation is regulatory approval, and science does not passively translate into useful medicinal products. Today, in solid cancer, response criteria related to tumor size are less useful than during the earlier cytotoxic drugs era; advanced imaging and biomarkers now allow for tracking of the natural history of the disease in the laboratory and the clinic. Also, it is difficult to infer clinical benefit from tumor shrinkage since it is rarely sustained. Accordingly, size-based response criteria may represent an anachronism relative to translation in solid cancer and it may be appropriate to align preclinical and clinical effort and shift the focus to local invasion and metastasis. The shift from a cancer cellcentric model to a stroma centric model offers novel opportunities not only to interupt the natural history of the disease, but also to rethink the relevance of outdated criteria of clinical response. Current evidence favors the opinion that, in solid cancer, a different, broader, and contextual approach may lead to interventions that could delay local invasion and metastasis. All elements supporting this shift, especially advanced imaging, are in place(AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Neoplasias/tratamento farmacológico , Neoplasias/imunologia , Antineoplásicos/uso terapêutico , Metástase Neoplásica/tratamento farmacológico , Adjuvantes Imunológicos , Fibroblastos , Fibroblastos/patologia , Pesquisa Médica Translacional/métodos , Pesquisa Médica Translacional/tendências , Receptores Proteína Tirosina Quinases/análise , Testes Imunológicos/tendências , Células Estromais , Células Estromais/patologia
19.
Trauma (Majadahonda) ; 25(4): 226-231, oct.-dic. 2014. ilus
Artigo em Espanhol | IBECS | ID: ibc-132824

RESUMO

Objetivo: Optimizar la técnica de cultivo de células del disco intervertebral en humanos y confirmar la expresión del Toll-like receptor 4 (TLR4 ). Material y método: Se cultivaron muestras de núcleo pulposo obtenidas durante la cirugía de hernia discal. Las células cultivadas de los nueve pacientes (todos con degeneración del disco moderada-grave en la escala de Pfirrmann) fueron observadas para determinar su capacidad de crecimiento. Las células cultivadas obtenidas se estudiaron con el fin de determinar su fenotipo mediante inmunotinción y rtPCR. La presencia de TLR4 fue comprobada por los mismos métodos. Resultados: Las células aisladas se sembraron a diferentes concentraciones. La concentración ideal se obtuvo para las condiciones óptimas del cultivo. Se confirmó que el fenotipo de las células fue condrocitario y se confirmó la presencia del receptor TLR4 en las células cultivadas. Conclusión: Se confirma la presencia tanto de ARNm como de la proteína del receptor TLR4 en los condrocitos del disco intervertebral. Este hallazgo allana el camino para la caracterización de las funciones de este receptor en los procesos inflamatorios de la hernia de disco (AU)


Objective: To optimize the technique of culturing human intervertebral disc cells, and to confirm the expression of toll-like receptor 4 (TLR4) in these cells. Material and method: Samples of nucleus pulposus obtained during disc hernia surgery were cultured. Cells from nine patients (all with moderate-severe disc degeneration scores, based on the Pfirmann scale) were followed up to determine their growing capacity. The obtained cultured cells were tested for the chondrocyte phenotype by immunostaining and rt-PCR and the presence of TLR4 was tested by the same methods. Results: The cells were isolated and seeded at different concentrations. The ideal concentration was obtained for optimal culture conditions. The cells were confirmed to have the chondrocyte phenotype and TLR4 was confirmed to be present in the cultured cells. Conclusion: This study confirms the presence of both mRNA and TLR4 protein in intervertebral disc chondrocytes. This paves the way for elucidating of the roles of this receptor in the inflammatory processes of disc hernia (AU)


Assuntos
Humanos , Masculino , Feminino , Condrócitos/citologia , Condrócitos/imunologia , Disco Intervertebral/citologia , Meios de Cultura/isolamento & purificação , Receptor 4 Toll-Like/análise , Imuno-Histoquímica/métodos , Imuno-Histoquímica , Expressão Gênica , Imuno-Histoquímica/instrumentação , Tripsina/análise , Fibroblastos/citologia , Fibroblastos
20.
Rev. neurol. (Ed. impr.) ; 59(4): 158-163, 16 ago., 2014. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-126337

RESUMO

Introducción. El mosaicismo diploide/triploide es una alteración cromosómica poco frecuente. La produce un fallo en la división poscigótica durante el desarrollo embrionario. Da lugar a la coexistencia de dos líneas celulares con diferente constitución cromosómica (46,XX y 69,XXX) en un mismo individuo. Su fenotipo clínico es característico. Las alteraciones pigmentarias con un patrón de distribución que sigue las líneas de Blaschko son el principal signo guía, así como las alteraciones de otros tejidos derivados del ectodermo. Casos clínicos. Describimos las características clínicas de tres pacientes afectos de mosaicismo diploide/triploide y realizamos una comparación de su fenotipo clínico con el de los casos publicados previamente en la bibliografía. Las alteraciones observadas con mayor frecuencia fueron alteraciones cutáneas, discapacidad intelectual, obesidad troncular, talla baja, hemihipertrofia, y manos pequeñas y estrechas con clino y camptodactilia. Las características fenotípicas de nuestros pacientes fueron similares a las de los casos comunicados previamente. Aunque no existe un fenotipo único y específico asociado al mosaicismo diploide/triploide, existen malformaciones características que conforman un síndrome malformativo bien definido. El cariotipo realizado en linfocitos de sangre periférica en las tres pacientes fue normal, y se logró el diagnóstico mediante cariotipo en fibroblastos cultivados tras biopsia de piel hipopigmentada. Conclusiones. La presencia de discapacidad intelectual asociada a obesidad troncular, talla baja, hemihipertrofia o clino y camptodactilia, además de las alteraciones cutáneas, debe hacer pensar en la posible existencia de un mosaicismo diploide/ triploide. En la mayoría de los casos, es necesario el estudio del cariotipo en los fibroblastos para llegar al diagnóstico (AU)


Introduction. Diploid/triploid mosaicism is a rare chromosomal abnormality. It is caused by a failure in the postzygotic division during embryonic development. It results in the coexistence of two genetically heterogeneous cell lines (46,XX and 69,XXX) in one individual. His clinical phenotype is characteristic. Pigmentary changes with a distribution pattern following Blaschko’s lines abnormalities in other ectoderm-derived tissues are the main diagnostic signs. Case reports. Three cases of diploid/triploid mosaicism are described, and compared to the previously reported cases. The most frequently observed symptoms were mental retardation, truncal obesity, short stature, hemihypertrophy, small and narrow hands with clino and camptodactyly. Phenotypic characteristics of our three patients were similar to those of previously reported cases. Although there is no single and specific phenotype associated with mosaicism diploid/triploid, there are some dysmorphic features that shape a recognizable malformative syndrome. Peripheral blood lymphocytes karyotype was normal in our patients. Diagnosis was reached performing a fibroblast karyotype from hypopigmented skin. Conclusions. Intellectual disability associated with truncal obesity, short stature, hemihypertrophy or clino/camptodactyly should suggest to clinicians the possible existence of a diploid/triploid mosaicism. In most cases, karyotype from fibroblasts is needed to reach the diagnosis (AU)


Assuntos
Humanos , Criança , Mosaicismo , Cariótipo , Fibroblastos , Hipopigmentação , Cariótipo Anormal , Transtornos Cromossômicos
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