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1.
Rev. osteoporos. metab. miner. (Internet) ; 11(2): 64-71, abr.-jun. 2019. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-188338

RESUMO

Objetivo: Analizar el efecto de los secretomas de tumores sólidos organotrópicos hacia el hueso en células de linaje osteogénico, de tipo osteoblástico y osteocítico, en la expresión de genes relacionados con el metabolismo óseo. Material y método: Caracterizamos los cambios en expresión génica por PCR cuantitativa a tiempo real del eje OPG/RANKL, así como de otros genes relacionados con la diferenciación osteoblástica como son Runx2 y osteocalcina, inducidos por los medios condicionados de células tumorales prostáticas, mama y melanoma en pre‐osteoblastosMC3T3‐E1 y osteocitos MLO‐Y4 murinos o en osteoblastos humanos, según correspondiese por especie. Resultados: La estimulación de las células osteocíticas con medios condicionados de células de melanoma o adenocarcinoma prostático indujo un incremento en la expresión génica de OPG y también de RANKL, viéndose incrementado la ratio OPG/RANKL. Únicamente el secretoma de las células de adenocarcinoma prostático alteró la expresión de Runx2en osteocitos. Los medios condicionados de células de cáncer de mama modificaron únicamente la expresión de RANKLen células osteoblásticas, viéndose disminuido la ratioOPG/RANKL. Conclusión: Los factores solubles tumorales tienen como diana celular a las células osteocíticas, favoreciendo la inducción de un nicho pre‐metastásico óseo por modificación de la ratio OPG/RANKL en el entorno óseo, y, con ello, la progresión de tumores organotrópicos óseos como son el melanoma y adenocarcinomas prostático


Objective: To analyze the effect of the secrets of solid organotropic tumors towards bone in osteogenic, osteoblastic and osteocytic lineage cells, in the expression of genes related to bone metabolism. Material and method: We characterize the changes in gene expression by quantitative real‐time PCR of the OPG/RANKL axis, as well as other genes related to osteoblastic differentiation such as Runx2 and osteocalcin, induced by the conditioned means of prostate tumor cells, breast and melanoma in pre MC3T3‐E1 osteoblasts and murine MLO‐Y4 osteocytes or in human osteoblasts, as appropriate by species. Results: Stimulation of osteocitic cells with conditioned means of melanoma or prostate adenocarcinoma cells inducedan increase in OPG and RANKL gene expression, with the OPG/RANKL ratio being increased. Only the secretome of prostate adenocarcinoma cells altered the expression of Runx2 in osteocytes. Conditioned media of breast cancer cellsonly modified the expression of RANKL in osteoblast cells, with a decrease in OPG/RANKL ratio. Conclusion: Soluble tumor factors have osteocitic cells as their cellular target, favoring the induction of a pre‐metastatic bone niche by modifying the OPG/RANKL ratio in the bone environment, and, thus, the progression of bone organo‐tropic tumors such as melanoma and prostatic adenocarcinomas


Assuntos
Humanos , Animais , Camundongos , Osteogênese/fisiologia , Metástase Neoplásica/patologia , Neoplasias Ósseas/patologia , Neoplasias Ósseas/metabolismo , Osteócitos/metabolismo , Osteoblastos/metabolismo , Regulação Neoplásica da Expressão Gênica , Reação em Cadeia da Polimerase em Tempo Real , Células Tumorais Cultivadas
3.
J. physiol. biochem ; 74(3): 395-402, ago. 2018. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-178994

RESUMO

Bone is a dynamic organ, the bone-forming osteoblasts and bone-resorbing osteoclasts form the physiological basis of bone remodeling process. During pathological process of numerous inflammatory diseases, these two aspects are uncoupled and the balance is usually tipped in favor of bone destruction. Evidence suggests that the inflammatory destruction of bone is mainly attributed to oxidative stress and is closely related to mitochondrial dysfunction. The mechanisms underlying osteogenic dysfunction in inflammation still need further investigation. Reactive oxygen species (ROS) is associated with mitochondrial dysfunction and cellular damage. Here, we reported an unexplored role of cyclophilin D (CypD), the major modulator of mitochondrial permeability transition pore (mPTP), and the CypD-mPTP axis in inflammation-induced mitochondrial dysfunction and bone damage. And the protective effects of knocking down CypD by siRNA interference or the addition of cyclosporin A (CsA), an inhibitor of CypD, were evidenced by rescued mitochondrial function and osteogenic function of osteoblast under tumor necrosis factor-alfa (TNF-alfa) treatment. These findings provide new insights into the role of CypD-mPTP-dependent mitochondrial pathway in the inflammatory bone injury. The protective effect of CsA or other moleculars affecting the mPTP formation may hold promise as a potential novel therapeutic strategy for inflammation-induced bone damage via mitochondrial pathways


No disponible


Assuntos
Animais , Camundongos , Ciclofilinas/metabolismo , Mitocôndrias/metabolismo , Proteínas de Transporte da Membrana Mitocondrial/metabolismo , Osteíte/metabolismo , Osteoblastos/metabolismo , Osteogênese , Estresse Oxidativo , Apoptose , Transporte Biológico Ativo , Biomarcadores/metabolismo , Ciclofilinas/antagonistas & inibidores , Ciclosporina/farmacologia , Imunossupressores/farmacologia , Mitocôndrias , Proteínas de Transporte da Membrana Mitocondrial/agonistas , Proteínas de Transporte da Membrana Mitocondrial/antagonistas & inibidores , Forma das Organelas
4.
J. physiol. biochem ; 74(1): 3-8, feb. 2018. graf
Artigo em Inglês | IBECS | ID: ibc-178912

RESUMO

The taste receptor type 1 (TAS1R) family of heterotrimeric G protein-coupled receptors participates in monitoring energy and nutrient status. TAS1R member 3 (TAS1R3) is a bi-functional protein that recognizes amino acids such as L-glycine and L-glutamate or sweet molecules such as sucrose and fructose when dimerized with TAS1R member 1 (TAS1R1) or TAS1R member 2 (TAS1R2), respectively. It was recently reported that deletion of TAS1R3 expression in Tas1R3 mutant mice leads to increased cortical bone mass but the underlying cellular mechanism leading to this phenotype remains unclear. Here, we independently corroborate the increased thickness of cortical bone in femurs of 20-week-old male Tas1R3 mutant mice and confirm that Tas1R3 is expressed in the bone environment. Tas1R3 is expressed in undifferentiated bone marrow stromal cells (BMSCs) in vitro and its expression is maintained during BMP2-induced osteogenic differentiation. However, levels of the bone formation marker procollagen type I N-terminal propeptide (PINP) are unchanged in the serum of 20-week-old Tas1R3 mutant mice as compared to controls. In contrast, levels of the bone resorption marker collagen type I C-telopeptide are reduced greater than 60% in Tas1R3 mutant mice. Consistent with this, Tas1R3 and its putative signaling partner Tas1R2 are expressed in primary osteoclasts and their expression levels positively correlate with differentiation status. Collectively, these findings suggest that high bone mass in Tas1R3 mutant mice is due to uncoupled bone remodeling with reduced osteoclast function and provide rationale for future experiments examining the cell-type-dependent role for TAS1R family members in nutrient sensing in postnatal bone remodeling


No disponible


Assuntos
Animais , Masculino , Reabsorção Óssea/metabolismo , Osso Cortical/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Células-Tronco Mesenquimais/metabolismo , Osteoblastos , Osteoclastos/metabolismo , Osteogênese , Receptores Acoplados a Proteínas-G/metabolismo , Biomarcadores/metabolismo , Reabsorção Óssea/imunologia , Reabsorção Óssea/patologia , Catepsina K , Linhagem Celular , Osso Cortical , Células-Tronco Mesenquimais/citologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Mutantes
5.
Clin. transl. oncol. (Print) ; 19(9): 1059-1066, sept. 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-165206

RESUMO

Osteoblasts are one among the critical components of the endosteal bone marrow (BM) niche. In addition to hematopoietic stem cell fate, their role in leukemogenesis as well as metastasis of a variety of cancers has been demonstrated in various studies. In this regard, endosteal niche can have a dual role as an initiator and protective role against leukemia. Knowledge of growth factors, chemokines and cytokines secreted by osteoblasts as well as their interaction with signaling pathways inform our understanding of the development, prognosis, recurrence and treatment of malignant BM diseases. Clinical progress in targeting the endosteal niche is also discussed (AU)


No disponible


Assuntos
Humanos , Osteoblastos/patologia , Medula Óssea/patologia , Nicho de Células-Tronco , Metástase Neoplásica/diagnóstico , Metástase Neoplásica/patologia , Leucemia/patologia , Prognóstico , Tratamento Farmacológico/instrumentação , Tratamento Farmacológico/métodos , Leucemia/diagnóstico , Leucemia/tratamento farmacológico , Antineoplásicos/uso terapêutico
6.
Med. oral patol. oral cir. bucal (Internet) ; 22(5): e651-e659, sept. 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-166662

RESUMO

Background: The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish. Material and Methods: An in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours. Results: A statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group). Conclusions: In comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it (AU)


No disponible


Assuntos
Humanos , Calcificação Fisiológica , Osteoblastos , Proteína Morfogenética Óssea 2/farmacocinética , Técnicas In Vitro , Estudos Prospectivos , Proteínas do Citoesqueleto/fisiologia , Apoptose/fisiologia , Matriz Óssea/crescimento & desenvolvimento , Sobrevivência Celular/fisiologia
9.
Rev. osteoporos. metab. miner. (Internet) ; 8(1): 5-14, ene.-mar. 2016. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-151228

RESUMO

Objetivos: Identificar microRNAs (miRNAs) diferencialmente expresados en muestras óseas con fractura osteoporótica respecto a huesos sanos. Material y métodos: Se extrajo RNA total a partir de hueso trabecular fresco del cuello femoral de mujeres sometidas a reemplazo de cadera, ya sea debido a fractura osteoporótica (n=6) o por artrosis en ausencia de osteoporosis (según la DMO) (n=6). Las muestras se hibridaron en un array de miRNAs y se realizaron diagramas de PCA y de mapa de calor. Para la comparación de los niveles de expresión, se fijó como significativo un umbral de cambio de >1,5 veces y un valor p <0,05 en la t de Student (corregido para múltiples pruebas). Resultados: Tanto los análisis de PCA como el mapa de calor mostraron una agrupación de las muestras según si eran de fractura o no. Se detectaron 790 miRNAs en las muestras de hueso, 82 de los cuales estaban alterados en las muestras osteoporóticas. Tras la validación en otro panel de 6 muestras osteoporóticas y 6 no osteoporóticas mediante PCR a tiempo real de los miRNAs más significativos, y para los que existía un ensayo disponible, se confirmaron los miRNAs miR-320a y miR-22-3p. Estos dos miRNAs se detectaron en cultivos de osteoblastos primarios, aunque no mantenían el mismo patrón de expresión que en las muestras de hueso total. Conclusiones: Hemos demostrado que existen diferencias en la expresión de miRNAs en muestras con fractura osteoporótica, lo que abre nuevas perspectivas para la investigación y diseño de nuevas terapias (AU)


Objectives: To identify microRNAs (miRNAs) differentially expressed in bone samples with osteoporotic fracture compared with healthy bones. Methods: Total RNA was extracted from fresh trabecular bone of the femoral neck of women undergoing hip replacement surgery, either because to osteoporotic fracture (n=6) or in the absence of osteoarthritis osteoporosis (based on BMD) (n=6). The samples were hybridized on an array of miRNAs and PCA diagrams and heat map were made. To compare expression levels, >1.5 times and a value p<0.05 Student's T test (corrected for multiple testing) was set as a threshold of significant change. Results: Both PCA analysis and the heat map showed a samples grouping whether there was fracture or not. 790 were detected miRNAs in bone samples, 82 of which were altered in the osteoporotic samples. After validation in another panel of 6 samples 6 osteoporotic and non-osteoporotic by PCR real time of the most significant miRNAs, and for which there was a test available, the miRNAs, miR-320a and miR-22-3p were confirmed. These two miRNAs were detected in cultures of primary osteoblasts, although they did not maintain the same pattern of expression in total bone samples. Conclusions: We have shown that there are differences in the expression of miRNAs in samples with osteoporotic fracture. This opens prospects for research and design of new therapies (AU)


Assuntos
Humanos , MicroRNAs/genética , Perfilação da Expressão Gênica , Osteoporose/genética , Fraturas por Osteoporose/genética , Osteoblastos/fisiologia , Osteoclastos/fisiologia
10.
Rev. esp. cir. ortop. traumatol. (Ed. impr.) ; 59(2): 122-128, mar.-abr. 2015. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-133875

RESUMO

Objetivo: El objetivo de este estudio es comparar la biocompatibilidad y efecto sobre osteoblastos de polimetilmetacrilato solo y PMMA al que se ha añadido, hidroxiapatita en concentraciones del 5, 10, 15 y 20%, no superando nunca esta cifra del 20%, dado que si se supera esta cifra pueden verse alteradas las propiedades biomecánicas del PMMA. Material y métodos: Estudio experimental que consiste en el estudio de la adhesividad, diferenciación y muerte celular sobre discos de PMMA y composite PMMA/HA a diferentes concentraciones. Resultados: Los composites al 15 y especialmente al 20% presentaron mejor respuesta osteoblástica, mayores marcadores de actividad y menores marcadores de apoptosis. En las imágenes de microcopía electrónica se aprecia una mayor adhesión celular (AU)


Objective: The purpose of this study is to compare the biocompatibility and the effect in osteoblasts of polymethyl methacrylate alone, and mixed with hydroxyapatite in different concentrations of 5, 10, 15 and 20%, without exceeding 20%, as it can alter mechanical properties of the composite. Material and methods: Experimental study comparing osteoblast response to Polymethyl methacrylate alone and with hydroxyapatite in different concentrations. Results: Composites at 15 and 20% obtained better osteoblast response, with higher osteoblastic activity markers, and lower apoptosis markers. Electron microscopy images show improved adhesion of osteoblasts (AU)


Assuntos
Humanos , Osteoblastos/fisiologia , Cimentos para Ossos/uso terapêutico , Fraturas da Coluna Vertebral/cirurgia , Fixação Interna de Fraturas/métodos , Osseointegração/fisiologia , Adesividade , Hidroxiapatitas/análise , Polimetil Metacrilato/análise , Resultado do Tratamento
11.
Clin. transl. oncol. (Print) ; 17(3): 173-179, mar. 2015. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-133304

RESUMO

Several cancers tend to metastasize to bone, leading to osteolytic or osteoblastic bone lesions. The respective phenotypes of bone destruction and bone formation vary in clinical features, including incidence, prognosis, skeletal-related events and bone biomarkers. In addition, different molecular mechanisms explain the difference in phenotype. For example, molecules involved in osteolytic bone metastases (represented with breast cancer) include parathyroid hormone-related protein, transforming growth factor-β, while in osteoblastic lesions (represented with prostate cancer), endothelin-1 and morphogenetic proteins, etc. play a more important role in bone formation. It is important for us to understand the differences of bone metastases between two phenotypes to help clinicians to understand the underlying mechanisms, behaviors and therapies in development and currently available for bone metastases (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Neoplasias Ósseas/diagnóstico , Metástase Neoplásica/patologia , Biomarcadores/análise , Osteólise/diagnóstico , Osteólise/patologia , Neoplasias Ósseas/epidemiologia , Neoplasias da Mama/complicações , Neoplasias Pulmonares/complicações , Reabsorção Óssea/diagnóstico , Reabsorção Óssea/patologia , Biotecnologia/métodos , Osteoblastos , Osteoblastos/patologia , Metástase Neoplásica/diagnóstico , Fenótipo , Prognóstico , Compressão da Medula Espinal/patologia , Compressão da Medula Espinal/radioterapia
12.
Rev. esp. cir. oral maxilofac ; 37(1): 15-22, ene.-mar. 2015. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-132507

RESUMO

Objective. To compare the use of Bone Marrow adult Stem Cells (BMSCs), differentiated in vitro into osteoblasts, associated to calcium phosphate versus autogenous bone graft, in the repair process of critical size bone defects. Materials and method. On 36 Wistar adult rats, bilateral full-thickness defects on parietal bone were created. The defects were either repaired with calcium phosphate (group I), calcium phosphate + (BMSCs) (group II) or autogenous bone graft (group III), and the opposite side with blood clot (Control Group). In all cases a collagen membrane was used. The animals were sacrificed at 30 and 60 days, and all specimens were collected for further histological and histomorfometric study. Results. At 30 days, group III (autogenous bone graft) evidences a statistical difference on bone formation when compared to the experimental and control groups (p ≤ 0.05). At 60 days group II (BS + BMSCs) and group III (autogenous bone) showed a similar bone formation and has only a statistical difference when compared to group I (BS) and control group. Conclusion. The use of calcium phosphate in conjunction with BMSCs resulted in a similar behavior in the process of bone repair in critical size defects, when compared with autogenous bone graft (AU)


Objetivo. Comparar el uso de células madre adultas de la médula ósea (CMMO), diferenciadas in vitro en osteoblastos, asociadas a fosfato cálcico, frente a injerto de hueso autólogo, en el proceso de reparación de defectos óseos de tamaño crítico. Material y Método. En 36 ratas adultas Wistar, se crearon defectos bilaterales de todo el grosor en el hueso parietal. Los defectos se repararon con fosfato de calcio (BoneSource®) (grupo I), fosfato de calcio (BoneSource®) + (CMMO) (grupo II) o injerto de hueso autólogo (grupo III), y en el lado contralateral con coágulo de sangre (grupo de control). En todos los casos se utilizó membrana de colágeno. Los animales fueron sacrificados a las 30 y 60 días y se obtuvieron todas las muestras para el estudio histológico y el análisis histomorfométrico. Resultados. A los 30 días, en el grupo III (injerto de hueso autólogo) se puso de manifiesto una diferencia estadísticamente significativa en la formación de hueso en comparación con el grupo experimental y el de control (p < 0,05). A los 60 días, en el grupo II (BoneSource®) + CMMO) y el grupo III (hueso autólogo) se demostró una formación ósea similar, y sólo se evidenció una diferencia estadísticamente significativa en comparación con el grupo I (BoneSource®) y el grupo de control. Conclusión. El uso de fosfato de calcio en combinación con CMMO indujo un comportamiento similar en el proceso de reparación ósea en defectos de tamaño crítico, en comparación con injerto de hueso autólogo (AU)


Assuntos
Animais , Masculino , Feminino , Ratos , Regeneração Óssea/fisiologia , Crânio/anormalidades , Crânio/cirurgia , Medula Óssea/anormalidades , Medula Óssea/cirurgia , Medula Óssea , Células-Tronco , Osteoblastos/patologia , Osteoblastos , Pirofosfato de Cálcio/uso terapêutico , Modelos Animais
13.
Med. oral patol. oral cir. bucal (Internet) ; 20(1): 88-93, ene. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-132062

RESUMO

Objectives: To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application. Study Design: Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: GroupI: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscope (SEM) and confocal laser scanning electron microscope( CLSM).Osteoblast adhesion, proliferation and cell morphology were determined by means of fluorescence microscopy with Image-Pro Plus software and SEM. Results: Group II presented the roughest discs, while the least rough were Group III. Cell adhesion was greatest in Group II. The addition of melatonin improved cell proliferation. Conclusions: 1. Surface treatments (dual acid etching, calcium phosphate impaction) increase surface roughness in comparison with machined titanium.2. Greater surface roughness tends to favor cell adhesion after 24-hour cell culture.3. The addition of melatonin tends to favor osteoblast proliferation (AU)


Assuntos
Humanos , Titânio/farmacocinética , Osteoblastos , Melatonina/farmacocinética , Osseointegração/fisiologia , Implantação Dentária/métodos , Teste de Materiais/métodos
14.
Med. oral patol. oral cir. bucal (Internet) ; 20(1): 111-116, ene. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-132065

RESUMO

Objective: The aim of this study was to determine whether the use of platelet rich fibrin (PRF) improved the healing of extraction sockets. Study Design: A total of 20 patients with bilateral soft tissue impacted mandibular third molars were included in this study. The left and right third molars were extracted during the same session. Subsequently, the PRF membrane was randomly administered to one of the extraction sockets, whereas the contralateral sockets were left without treatment. On postoperative 30. and 90. days, panoramic images and bone scintigrams were taken to evaluate the bone healing between PRF-treated and non-PRF-treated sockets. Also, periodontal evaluation was performed in the same control sessions. Dependent group t test for paired samples was used for statistical analysis. Results: The average increase in technetium-99m methylene diphosphonate uptake as an indication of enhanced bone healing did not differ significantly between PRF-treated and non-PRF-treated sockets 30 and 90 days postoperatively. Radioopacity that can show the bone healing on panoramic images were measured by Image J programme and they did not differ significantly. Also periodontal values did not differ significantly. Conclusions: PRF might not lead to enhanced bone healing in impacted mandibular third molar extraction sockets30 and 90 days after surgery. It is thought that PRF has the potential characteristics of an autologous fibrin matrix and can accelerate the healing. To better understand the effects of PRF on healing, further research is warranted with larger sample sizes (AU)


Assuntos
Humanos , Osteoblastos/fisiologia , Extração Dentária/métodos , Plasma Rico em Plaquetas , Adesivo Tecidual de Fibrina/uso terapêutico , Alvéolo Dental/cirurgia , Cicatrização/fisiologia , Dente Serotino/cirurgia , Resultado do Tratamento
16.
Artigo em Inglês | IBECS | ID: ibc-118252

RESUMO

Background: Sexual hormones have an important role in many hormone-dependant tumors like breast and prostate carcinomas, and also a relationship has been found with bone metabolism and bone tumors. Some studies have demonstrated that the expression of hormonal receptors (HR) in osteosarcomas (OS) of long bones is associd with gender, histological grade, histological type, and possibly may be connection with pathogenesis and evolution. However, to our knowledge there are no studies of HR in osteosarcomas of craniofacial bones (OS-CF).Objectives: To assess the expression of hormonal receptors in OS-CF. Material and Methods: Twenty one cases of OS-CF were included in this study. Clinical outcome was obtained from clinical charts. Histological sections were reviewed, and immunohistochemistry studies for estrogen, progesterone and androgen receptors were performed. Results: A striking female predominance was found (2:1), with a median age of 35 years. The predominant type of OS was osteoblastic (52.4%), and histological grade was high in 86%. Follow-up was obtained in 13 cases and ranged from 6 to 118 months (median 29 months). There were 8 patients (61.5%) dead or alive with progressive disease in the last follow up. Negative expression of HR was found in 19/21 cases; one showed weak nuclear expression for estrogen receptor, and another for androgen receptor. Progesterone receptor was negative in all cases. Conclusions: OS-CF mostly affected females, most of them were of the osteoblastic type and of high grade. Hormonal expression was practically negative in osteosarcoma of craniofacial bones (AU)


Assuntos
Humanos , Receptores de Esteroides/análise , Osteossarcoma/diagnóstico , Receptores Androgênicos/análise , Neoplasias Maxilomandibulares/diagnóstico , Osteoblastos , Biomarcadores Tumorais/análise
17.
Rev. esp. cir. ortop. traumatol. (Ed. impr.) ; 57(6): 384-390, nov.-dic. 2013.
Artigo em Espanhol | IBECS | ID: ibc-116864

RESUMO

Introducción. Existen muchos estudios referentes a los diferentes efectos producidos por el fresado intramedular en el callo de fractura, pero no existe evidencia en la literatura del efecto de dicho fresado en la osteogénesis. Realizamos un estudio prospectivo para analizar el efecto del fresado endomedular y enclavado en la producción de factores de crecimiento durante el proceso de consolidación de la fractura en el fémur producida en ratas. Material y métodos. Producimos una fractura diafisaria, transversa, no conminuta de fémur en 64 ratas: 34 ratas no recibieron ningún tratamiento y las otras 30 se trataron mediante un procedimiento quirúrgico estandarizado, consistente en fresado del canal medular de distal a proximal y fijación de la fractura con una aguja de Kirschner. Las ratas fueron sacrificadas a las 24 h, 4.°, 7.° y 15.° días después de la fractura. Medimos la cantidad de factores de crecimiento (PDGFA, TGF2 y TGFBeta-R2) en el callo de fractura mediante estudio anatomopatológico en los diferentes momentos del sacrificio. Resultados. Los resultados de las variables primarias del estudio, estratificadas por tiempo hasta el sacrificio, no mostraron diferencias estadísticas significativas. Discusión. Aunque la presencia de una aguja intramedular facilita la estabilización de la fractura y la formación del callo de fractura, no hemos encontrado ninguna evidencia significativa de que el fresado endomedular produzca cambios en la expresión de los factores de crecimiento estudiados (TGFBEta-R2, PDGFA y TGFBeta2) durante la formación del callo de fractura de fémur en ratas (AU)


Introduction: Many studies have been conducted to determine the different effects that reaming or intramedullary nailing have on fracture healing, but there is no evidence in the literature of the effect of intramedullary reaming on osteogenesis. We performed a prospective study to analyse the effect of intramedullary reaming and nailing on the production of growth factors during the process of fracture healing in the femur of rats. Material and methods: A transverse mid-shaft non-comminuted femur fracture was produced in 64 rats; 34 rats did not receive any treatment, and a standardized surgical procedure was performed on 30 rats, by exposing the left knee, reaming the medullary canal from distal to proximal, and then fixing the fracture with a steel pin. The rats were sacrificed at the 24th hour, 4th, 7th and 15th days after the fracture. The amount of growth factors that appeared in the callus fracture was measured using histopathology studies. The primary categorical variables analysed were PDGFA, TGF2 and TGFBeta-R2. These variables were analysed in each group at the different sacrifice times. Results: The results of the primary variables of the study, stratified by the time until sacrifice, showed no statistically significant differences. Discussion: Even if the presence of an intramedullary wire facilitates the fracture repair and the stabilising the bridge of bone between both edges of the fracture site, no evidence was found that reaming changes the expression of the growth factors studied (PDGFA, TGFBeta-R2 and TGFBeta) during the callus formation in rats (AU)


Assuntos
Animais , Masculino , Feminino , Ratos , Fraturas do Fêmur/diagnóstico , Fraturas do Fêmur/cirurgia , Fraturas do Fêmur/veterinária , Fêmur , Calo Ósseo/patologia , Calo Ósseo/cirurgia , Calo Ósseo , Período Perioperatório/métodos , Período Perioperatório/tendências , Período Perioperatório/veterinária , Anestesia/métodos , Anestesia , Fêmur/lesões , Fêmur/fisiopatologia , Osteoblastos/citologia , Microanálise por Sonda Eletrônica/tendências , Microscopia/métodos , Microscopia/normas , Microscopia
18.
Reumatol. clín. (Barc.) ; 9(5): 303-315, sept.-oct. 2013. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-115101

RESUMO

El tejido óseo es una estructura fuertemente regulada, que desempeña un papel esencial en diferentes funciones fisiológicas. A través de acciones autocrinas y paracrinas participa en la hematopoyesis, influyendo en el destino de las células madre hematopoyéticas. Existe además una serie de moléculas compartidas y también múltiples conexiones entre el sistema inmune y el tejido óseo. Con el objetivo de agrupar los conocimientos que relacionan ambos sistemas, se ha desarrollado una nueva disciplina conocida con el término «osteoinmunología». Su progresión en los últimos años ha sido exponencial y nos ha permitido relacionar e incrementar el conocimiento en temas aparentemente alejados como la erosión reumatoide, la osteoporosis posmenopáusica, las metástasis óseas o la enfermedad periodontal. En la presente revisión hemos tratado de resumir los avances más relevantes que se han producido en la última década, sobre todo en aquellos aspectos que interesan de manera preferente a la reumatología(AU)


Bone tissue is a highly regulated structure, which plays an essential role in various physiological functions. Through autocrine and paracrine mechanisms, bone tissue is involved in hematopoiesis, influencing the fate of hematopoietic stem cells. There are a number of molecules shared by bone cells and immune system cells indicating that there are multiple connections between the immune system and bone tissue. In order to pool all the knowledge concerning both systems, a new discipline known under the term «osteoimmunology» has been developed. Their progress in recent years has been exponential and allowed us to connect and increase our knowledge in areas not seemingly related such as rheumatoid erosion, postmenopausal osteoporosis, bone metastases or periodontal disease. In this review, we have tried to summarize the most important advances that have occurred in the last decade, especially in those areas of interest related to rheumatology(AU)


Assuntos
Humanos , Masculino , Feminino , Sistema Imunitário/imunologia , Sistema Imunitário/patologia , Osso e Ossos/imunologia , Osteoporose/epidemiologia , Osteoporose/prevenção & controle , Linfócitos/imunologia , Artrite Reumatoide/epidemiologia , Artrite Reumatoide/prevenção & controle , Osteoblastos/imunologia , Osteoporose Pós-Menopausa/epidemiologia , Osteoporose Pós-Menopausa/prevenção & controle
20.
Arch. esp. urol. (Ed. impr.) ; 66(5): 463-474, jun. 2013. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-113260

RESUMO

Las metástasis óseas son complicaciones frecuentes y a menudo desvastadoras de los pacientes con cáncer. Recientemente se han producido avances significativos en nuestra comprensión de los mecanismos moleculares responsables de metástasis tanto osteolíticas como osteoblásticas. El uso de las “OMICAS” y la disponibilidad de modelos animales preclínicos de metástasis óseas adecuados han permitido la identificación de los factores producidos por el tumor o por el estroma en respuesta al tumor. Este tipo de estudios deberían resultar en una disminución de las morbilidades esqueléticas graves asociadas con el cáncer de próstata metastásico y pueden en el futuro mejorar la supervivencia general. En esta revisión, se resumirá las dianas moleculares en las metástasis óseas de nueva generación (AU)


Bone metastases are a frequent and devastating complication in cancer patients. Recently, significant advances in our understanding of the molecular mechanisms responsible for both osteolytic and osteoblastic bone metastases have occurred. The use of OMICS and the availability of appropriate preclinical animal models of bone metastasis have permitted the identification of factors produced by the tumor or by the host stroma in response to the tumor. These types of studies should result in a decrease of the serious skeletal morbidities associated with metastatic prostate cancer and may in the future improve overall survival. In this review the next generation of molecular targets in bone metastasis will be summarized (AU)


Assuntos
Humanos , Patologia Molecular/métodos , Neoplasias Ósseas/secundário , Metástase Neoplásica/patologia , Biologia Molecular/tendências , Osteoclastos , Osteoblastos
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