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2.
Rev. senol. patol. mamar. (Ed. impr.) ; 32(1): 17-25, ene.-mar. 2019. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-187029

RESUMO

A pesar de los avances conseguidos en el tratamiento del cáncer de mama, todavía sigue siendo una causa importante de mortalidad en las mujeres. Por tanto, resulta necesario plantear nuevos enfoques de la fisiopatología de la enfermedad que contribuyan a realizar una mejor evaluación pronóstica, y a la mejora de las estrategias terapéuticas. Para ello, deberíamos considerar que el cáncer no es solo una transformación maligna de las células epiteliales y su progresión meramente autónoma; sino que, hoy en día, existen datos que apoyan el concepto del cáncer como un ecosistema basado en una sociología de diferentes tipos celulares, con sus interacciones complejas. Entre los diversos tipos de células que conforman el estroma tumoral, y que tienen un papel relevante en la progresión del cáncer de mama, se encuentran los fibroblastos asociados al cáncer, las células inflamatorias y las células endoteliales. Existen diferentes factores moleculares expresados por esas células que se asocian con el desarrollo de metástasis, tales como las metaloproteasas de matriz y sus inhibidores tisulares, citoquinas o receptores tipo toll. En base a la expresión de todos ellos, aquí proponemos 2 fenotipos de estroma del cáncer de mama con influencias marcadamente diferentes sobre el pronóstico de las pacientes. También analizamos los mecanismos involucrados en la interrelación tumor-estroma que pueden llevarnos a mejorar las estrategias terapéuticas en el cáncer de mama


Despite advances in the treatment of breast cancer, it remains an important cause of mortality in women. Therefore, it is necessary to propose new approaches to the pathophysiology of the disease that could help to improve prognostic evaluation and therapeutic strategies. To do this, we should consider that cancer is not only a malignant transformation of the epithelial cells or their purely autonomous growth; nowadays, there are data that support the concept of cancer as a system based on a sociology of different cell types, with complex interactions. Among the various types of cells that make up the tumour stroma and which play an important role in the progression of breast cancer are cancer-associated fibroblasts, inflammatory cells and endothelial cells. Several molecular factors expressed by these cells are associated with the development of metastases, such as matrix metalloproteases and their tissue inhibitors, cytokines or toll-like receptors. Based on the expression of all of these factors, here we propose two types of stroma from breast cancer that display markedly different influences on patient prognosis. We also analyse mechanisms involved in the tumour-stroma interrrelationship that could help to improve therapeutic strategies in breast cancer


Assuntos
Humanos , Células Estromais/patologia , Neoplasias da Mama/patologia , Fibroblastos/patologia , Células Endoteliais/patologia , Mediadores da Inflamação/análise , Prognóstico , Receptores Toll-Like/análise , Junções Intercelulares/patologia
3.
Clin. transl. oncol. (Print) ; 20(9): 1185-1195, sept. 2018. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-173704

RESUMO

Purpose: Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide, and its outcome is poor. The purpose of this study was to determine the association between JNK1 and vitamin D receptor (VDR) expression and the prognosis of ESCC. Methods: Immunohistochemical staining was conducted on ESCC tissue microarrays (362 pairs of ESCC and normal esophagus tissues). The epithelial and stromal expression levels of c-jun NH2-terminal kinase 1 (JNK1) and VDR were scored and correlated with the ESCC characteristics. Laser-capture-based quantitative RT-PCR was performed on ESCC tissues. The effects of JNK1 and VDR on ESCC cell proliferation and migration were analyzed in vitro by transient transfection, and protein changes were evaluated by immunoblotting. Results: Both JNK1 and VDR were reduced in ESCC epithelial cells in comparison with the normal esophagus, but the expression of JNK1 and VDR in ESCC stromal tissues, not epithelial cells, was strongly associated with the survival time of ESCC patients. Functional studies showed that increased JNK1 suppressed cancer cell proliferation, mobility, and migration, which were linked to the alterations of VDR and metastasis-associated proteins. Conclusion: JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinoma


No disponible


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Células Estromais/patologia , Neoplasias Esofágicas/patologia , Carcinoma de Células Escamosas/patologia , Proteínas Quinases JNK Ativadas por Mitógeno/isolamento & purificação , Receptores de Calcitriol/isolamento & purificação , Prognóstico , Biomarcadores Tumorais/análise , Proteínas Supressoras de Tumor/isolamento & purificação
5.
Cir. plást. ibero-latinoam ; 43(4): 381-386, oct.-dic. 2017. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-170453

RESUMO

Introducción y Objetivo. El tejido adiposo es una fuente de células estromales de fácil acceso. Para su almacenamiento y posterior aplicación en clínica es importante que el método de criopreservación a utilizar mantenga adecuadas tasas de viabilidad tras un ciclo de congelación. El objetivo del presente trabajo es obtener células estromales humanas derivadas del tejido adiposo, implementar un protocolo de criopreservación libre de proteína animal y medir las tasas de viabilidad posterior a un ciclo de criopreservación para su eventual aplicación clínica. Material y Método. Utilizamos grasa fresca de 5 pacientes sometidas a lipoaspiración y aislamos las células estromales mediante técnicas de digestión enzimática y expansión celular. Realizamos la caracterización de las células mediante inmunofenotipificación. Criopreservamos las células utilizando dimetil-sulfóxido 10% y las almacenamos durante 1 mes en nitrógeno líquido. Evaluamos la tasa de viabilidad mediante ioduro de propidio y citometría de flujo, antes y después de un ciclo de criopreservación. Resultados. Obtuvimos células estromales derivadas del tejido adiposo, confirmado con el panel de inmunofenotipificación. Las tasas de viabilidad promedio obtenidas con ioduro de propidio fue 61.89% mientras que la tasa de mortalidad fue 32.68% tras un ciclo de criopreservación. Conclusiones. Mediante un protocolo de criopreservación utilizando un medio definido con dimetil-sulfóxido 10% en ausencia de proteína animal, es posible obtener tasas aceptables de viabilidad de las células estromales derivadas del tejido adiposo tras un ciclo de criopreservación (AU)


Background and Objective. Adipose tissue is an easy access source of stromal cells. For storage and subsequent clinical application, it is important that the method of cryopreservation used maintain adequate viability rates after a cycle of freezing. Our aim is to get stromal cells derived from human adipose tissue, implement a protocol of cryopreservation free of animal protein and subsequent measure viability rates after a cryopreservation cycle for its posterior clinical use. Methods. Fresh adipose tissue of 5 patients undergoing liposuction was used and stromal cells were isolated by enzymatic digestion techniques and cell expansion. Characterization of the cells was performed by immunophenotyping. Cells using 10% dimethylsulfoxide and stored for 1 month were cryopreserved in liquid nitrogen. Viability rates were assessed by propidium iodide and flow cytometry before and after a cryopreservation cycle. Results. Stromal cells derived from adipose tissue, confirmed immunophenotyping panel, were obtained. The average viability rates obtained with propidium iodide was 61.89% while the mortality rate was 32.68% after a cryopreservation cycle. Conclusions. Using a cryopreservation protocol with a defined medium with 10% dimethylsulfoxide and animal protein free, it is possible to obtain acceptable viability rates of stromal cells derived from adipose tissue after a cryopreservation cycle (AU)


Assuntos
Humanos , Feminino , Adulto , Células Estromais , Tecido Adiposo/cirurgia , Transplante de Células-Tronco/métodos , Gordura Abdominal/cirurgia , Imunofenotipagem/métodos , Citometria de Fluxo/métodos , Índice de Massa Corporal
6.
Rev. esp. patol ; 50(4): 229-233, oct.-dic. 2017. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-166038

RESUMO

Recientemente se ha descrito un tumor placentario que se ha denominado corangiocarcinoma. Consiste en una proliferación vascular, con células estromales, rodeadas por una neoplasia trofoblástica atípica. La proliferación vascular y estromal se pone de manifiesto con CD-34 y vimentina. Las células trofoblásticas atípicas, pleomórficas con frecuentes mitosis son positivas para CK 8-18, panCK y BHCG. Es difícil distinguir de un tumor de colisión del tipo corangioma-coriocarcinoma. Hasta el momento actual se han descrito menos de diez casos de esta entidad en la literatura. Tras el seguimiento realizado en cada caso no se han observado metástasis en las progenitoras ni en los descendientes (AU)


Chorangiocarcinoma is a recently described placental tumour showing a vascular proliferation with stromal cells surrounded by an atypical trophoblastic neoplasia. The stromal and vascular proliferation is demonstrated with CD-34 and Vimentin. Atypical pleomorphic trophoblastic cells with frequent mitosis are positive for CK-8-18, pan CK and BCHG. It is difficult to differentiate this carcinoma from a collision tumour of the corangioma-choriocarcinoma type. To date, less than ten cases of this tumour have been reported but follow ups did not reveal any metastases in either mother or child in any case (AU)


Assuntos
Humanos , Feminino , Adulto , Doença Trofoblástica Gestacional/patologia , Células Estromais/patologia , Tumores do Estroma Endometrial/patologia , Coriocarcinoma/patologia , Placenta/patologia , Hemorragia Uterina/complicações , Hemorragia Uterina/patologia , Programas de Rastreamento/métodos , Mitose
7.
Actas urol. esp ; 41(6): 376-382, jul.-ago. 2017. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-164453

RESUMO

Objetivo: Analizar el comportamiento de la metaloprotesa 11 (MMP11) en fibroblastos cultivados procedentes de tumores prostáticos humanos con diferentes características clinicopatológicas. Material y métodos: Para este estudio se analizaron muestras de biopsias de próstata obtenidas por vía transrectal de tumores con diferentes características, tratados o no con privación androgénica (PA). Tras la optimización del método de cultivo, se aislaron y cultivaron los fibroblastos para realizar el estudio (PCR) del ARNm de MMP11. Resultados: Se estudiaron finalmente 37 casos: 5 muestras de hiperplasia benigna de próstata, 14 casos con neoplasias localizadas (7 de alto riesgo según la clasificación de D’Amico), 5 con tumores con metástasis óseas y 13 tratados con PA, de los que 6 cumplían los requisitos para ser definidos como resistentes a la castración. En los tumores sin PA, la expresión de MMP11 fue significativamente mayor (p = 0,001) en los fibroblastos de tumores de grados más altos. Se encontró una correlación significativa (p = 0,001) entre PSA y expresión de MMP11 fibroblástica y un aumento significativo de la expresión de MMP11 en los tumores metastásicos. En los tumores con PA se objetivó una expresión significativamente mayor de MMP11 en pacientes resistentes a la castración que en los sensibles a esta (p = 0,003). Conclusión: En tumores de próstata avanzados o en fases de mayor agresividad tumoral, la producción de MMP11 por los fibroblastos resulta significativamente mayor que en tumores no metastásicos o en fases de sensibilidad a la PA


Objective: To analyze the expression of metalloprotein 11 (MMP11) in cultured fibroblasts obtained from human prostate tumors with different clinical and pathological characteristics. Material and methods: For this study we analyzed samples of transrectal prostate biopsies from tumors with different characteristics, treated with or whithout androgen deprivation (AD). After optimization of the culture method, fibroblasts were isolated and cultured to perform the study (PCR) of MMP11 mRNA. Results: Finally, 37 cases were studied: 5 samples of benign prostatic hyperplasia, 14 cases with localized neoplasms (7 high-risk according to the D’Amico classification), 5 with metastasic tumors (bone metastases), and 13 treated with AD therapy, of which 6 fulfilled the requirements to be defined as resistant to castration. In tumors without AD therapy, MMP11 expression was significantly higher (P= .001) in fibroblasts of higher grade tumors. A significant (P= .001) correlation was found between PSA and expression of MMP11 in fibroblast s and a significant increase of MMP11 expression in metastatic tumors. In tumors with AD therapy, a significantly greater expression of MMP11 was observed in resistant to castration patients than in those sensitive to castration (P= .003). Conclusion: In advanced prostate tumors or in stages of increased tumor aggressiveness, the production of MMP11 by fibroblasts is significantly greater than in non-metastatic tumors or in AD sensitive tumors


Assuntos
Humanos , Masculino , Neoplasias da Próstata/patologia , Metaloproteinase 11 da Matriz/análise , Neoplasias de Próstata Resistentes à Castração/patologia , Biomarcadores Tumorais/análise , Estadiamento de Neoplasias , Fibroblastos/ultraestrutura , Células Estromais/patologia
8.
Arch. esp. urol. (Ed. impr.) ; 70(6): 617-620, jul.-ago. 2017. ilus
Artigo em Espanhol | IBECS | ID: ibc-164566

RESUMO

Objetivo: Describir y conocer los tumores de células de la granulosa testicular del adulto (TCG) (clasificado como tumores del estroma y cordones sexuales de las gónadas) ya que conforman una variante rara con pocos casos publicados y de comportamiento clínico poco conocido. Método; Presentación de un nuevo caso de TCG testicular del adulto en un varón de 59 años, asintomático, con hallazgo ecográfico de una masa intratesticular de 3,3 cm heterogénea, con áreas sólidas y quísticas; marcadores tumorales y estudio de extensión negativos. Resultado: Confirmación del caso con análisis anatomopatológico e inmunohistoquímico, similar a su homólogo ovárico. Conclusiones: El TCG del adulto, es un tumor infrecuente de comportamiento incierto. Aunque suele cursar con buen pronóstico, se ha descrito su potencial metastásico incluso años después de la orquiectomía, por lo que requiere un seguimiento a largo plazo (AU)


Objective: To describe the adult type granulosa cell testicular tumors (classified as sex cord-stromal tumor) due to their behavior, hardly known with a small number of cases reported. Method: We report a new case of a 59-year-old man presenting an adult type granulosa cell tumor of the testis (AGCTT), painless, with a 3.3 centimeter intratesticular heterogeneous mass on ultrasound, with solid and cystic areas. Serum tumor markers and extension study were negative. Results: Histologic and inmunohistochemical studies confirmed an AGCTT, similar to its ovarian counterpart. Conclusion: AGCTT are rare neoplasms with unpredictable behavior. Their metastatic potential has been described, reason why they need a long follow-up; however, they usually have a good prognosis (AU)


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Tumor de Células da Granulosa/patologia , Neoplasias Testiculares/patologia , Orquiectomia , Células Estromais/patologia , Cordão Espermático/patologia
9.
Eur. j. anat ; 21(2): 97-112, abr. 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-163135

RESUMO

The gastrointestinal stromal tumour (GISTs), the most common mesenchymal neoplasm in the gastrointestinal tract, has been the subject of great interest in recent years in terms of prognosis, diagnosis and treatment. Its etiology is linked to the mutation of c-KIT and PDGFRA genes, although between 5 and 15% show no signs of such mutations. It is still diagnosed using immunohistochemical staining. The first line of treatment continues to be surgery, although advances in the molecular biology of GISTs are facilitating the development of new treatment strategies. Those that act by regulating tyrosine kinase activity are of particular interest. Drugs such as imatinib and sunitinib have improved the prognosis of these patients, although the development of resistance constitutes one of the main limitations of the treatment. The aim of this review is to present an up-to-date overview of the main etiopathogenic, diagnostic and therapeutic aspects of these tumours


No disponible


Assuntos
Humanos , Tumores do Estroma Gastrointestinal/patologia , Mesoderma/patologia , Neoplasias Gastrointestinais/patologia , Células Estromais/patologia , Nanotecnologia/métodos , Proteínas Proto-Oncogênicas c-kit/análise , Marcadores Genéticos
10.
J. physiol. biochem ; 73(1): 1-4, feb. 2017. ilus
Artigo em Inglês | IBECS | ID: ibc-168387

RESUMO

Obesity is a leading health problem facing the modern world; however, no effective therapy for this health issue has yet been developed. A promising research direction to identify novel therapies to prevent obesity has emerged from discoveries on development and function of brown/brite adipocytes in mammals. Importantly, there is evidence for the presence and function of active thermogenic brown adipocytes in both infants and adult humans. Several new investigations have shown that thermogenic adipocytes are beneficial to maintain glucose homeostasis, insulin sensitivity, and a healthy body fat content. Such thermogenic adipocytes have been considered as targets to develop a therapy for preventing obesity. This short review seeks to highlight recent findings on the development and function of brown/brite adipocytes in humans and to discuss potential treatments based on these adipocytes to reduce obesity and its related disorders (AU)


No disponible


Assuntos
Humanos , Animais , Adipócitos Bege/metabolismo , Adipócitos Marrons/metabolismo , Adipogenia , Modelos Biológicos , Termogênese , Adipócitos Brancos , Adiposidade , Capilares/citologia , Resistência à Insulina , Obesidade , Células Estromais/citologia
12.
Rev. esp. patol ; 49(1): 62-65, ene.-mar. 2016. ilus
Artigo em Espanhol | IBECS | ID: ibc-149069

RESUMO

Los tumores de células de la granulosa (TCG) pertenecen al grupo de tumores del estroma y cordones sexuales de las gónadas. Se distinguen 2 tipos: juvenil y adulto. Los TCG de tipo adulto testicular son extremadamente raros, y solo un pequeño número de casos han sido publicados. Su comportamiento biológico es incierto, con casos descritos de metástasis a distancia años después de su presentación inicial. Su diagnóstico se basa en el reconocimiento de las características citológicas y arquitecturales descritas en su homólogo ovárico, ya que ambos son morfológicamente indistinguibles. Describimos un nuevo caso de TCG de tipo adulto testicular en un varón de 23 años, haciendo hincapié en su comportamiento clínico, así como en las características inmunofenotípicas de este raro tumor (AU)


Granulosa cell tumors (GCT) belong to the group of sex cord-gonadal stromal tumours. Two types can be differentiated: juvenile and adult. Adult testicular GCT are extremely rare with only a small number of published cases. Their biological behaviour is not completely clear with cases of distant metastasis appearing years after the initial diagnosis. Diagnosis is based on the cytological and architectural characteristics described in its ovarian counterpart, since both are morphologically indistinguishable. We present a further case of GCT of the adult type in a 23 year old man. We discuss the clinical behavior and immunophenotypical characteristics of this rare tumour (AU)


Assuntos
Humanos , Masculino , Adulto , Tumor de Células da Granulosa/complicações , Tumor de Células da Granulosa/diagnóstico , Tumor de Células da Granulosa/patologia , Neoplasias Testiculares/patologia , Células Estromais , Tumores do Estroma Gonadal e dos Cordões Sexuais/patologia , Neoplasias Testiculares , Testículo/patologia , Testículo
14.
Rev. esp. patol ; 48(1): 35-40, ene.-mar. 2015. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-132464

RESUMO

El tumor del estroma gastrointestinal (GIST) es la neoplasia mesenquimal gastrointestinal más frecuente. Desde el descubrimiento de la inmunoexpresión de c-KIT y la existencia de mutaciones en KIT y PDGFRA ha sido un ejemplo de terapia molecular dirigida que permitió la aplicación de un tratamiento, el mesilato de imatinib, altamente eficaz. Desde entonces hay evidencia de que en realidad el GIST constituye un grupo heterogéneo desde el punto de vista clínico, morfológico, inmunohistoquímico y molecular. Esta variabilidad puede implicar distintos acercamientos diagnósticos y terapéuticos para cada caso particular, por lo que se debe establecer cuando hay que realizar estudio molecular. Es también imprescindible valorar el riesgo de recurrencia y metástasis para establecer un correcto pronóstico y por la posibilidad de utilizar terapia adyuvante. En este artículo se revisan tanto las características clínicas, morfológicas, inmunohistoquímicas y moleculares del GIST atendiendo a su complejidad, como la correcta valoración del riesgo (AU)


Gastrointestinal stromal tumor (GIST) is the most common gastrointestinal mesenchymal neoplasm. Since the discovery of c-KIT immunoexpression and the presence of KIT and PDGFRA mutations, it has represented an example of molecular targeted therapy that allowed the use of a highly effective treatment: imatinib mesylate. Since then there is evidence that GIST actually constitute a rather heterogeneous group from the clinical, morphological, immunohistochemical and molecular point of view. This variability may imply possible different diagnostic and therapeutic approaches for each specific case, making necessary to establish when molecular analyses must be performed. Is also mandatory to evaluate the risk of recurrence and metastases not only to know the prognosis but because adjuvant therapy may be used. n this paper the clinical, morphological, immunohistochemical and molecular features of GIST and the correct evaluation of risk are reviewed attending to its complexity (AU)


Assuntos
Humanos , Masculino , Feminino , Células Estromais/patologia , Tumores do Estroma Gastrointestinal/diagnóstico , Tumores do Estroma Gastrointestinal/patologia , Imuno-Histoquímica/métodos , Imuno-Histoquímica/tendências , Imuno-Histoquímica , Patologia Molecular/métodos , Patologia Molecular/tendências , Quimioterapia Adjuvante , Técnicas de Diagnóstico do Sistema Digestório , Neoplasias Gastrointestinais/diagnóstico , Neoplasias Gastrointestinais/patologia , Patologia Molecular/instrumentação , Patologia Molecular/organização & administração , Patologia Molecular/normas
15.
Clin. transl. oncol. (Print) ; 17(1): 1-10, ene. 2015. ilus
Artigo em Inglês | IBECS | ID: ibc-131899

RESUMO

The purpose of translation is the development of effective medicinal products based on validated science. A parallel objective is to obtain marketing authorization for the translated product. Unfortunately, in solid cancer, these two objectives are not mutually consistent as evidenced by the contrast between major advances in science and the continuing dismal record of pharmaceutical productivity. If the problem is unrelated to science, then the process of translation may require a closer examination, namely, the criteria for regulatory approval. This realization is important because, in this context, the objective of translation is regulatory approval, and science does not passively translate into useful medicinal products. Today, in solid cancer, response criteria related to tumor size are less useful than during the earlier cytotoxic drugs era; advanced imaging and biomarkers now allow for tracking of the natural history of the disease in the laboratory and the clinic. Also, it is difficult to infer clinical benefit from tumor shrinkage since it is rarely sustained. Accordingly, size-based response criteria may represent an anachronism relative to translation in solid cancer and it may be appropriate to align preclinical and clinical effort and shift the focus to local invasion and metastasis. The shift from a cancer cellcentric model to a stroma centric model offers novel opportunities not only to interupt the natural history of the disease, but also to rethink the relevance of outdated criteria of clinical response. Current evidence favors the opinion that, in solid cancer, a different, broader, and contextual approach may lead to interventions that could delay local invasion and metastasis. All elements supporting this shift, especially advanced imaging, are in place(AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Neoplasias/tratamento farmacológico , Neoplasias/imunologia , Antineoplásicos/uso terapêutico , Metástase Neoplásica/tratamento farmacológico , Adjuvantes Imunológicos , Fibroblastos , Fibroblastos/patologia , Pesquisa Médica Translacional/métodos , Pesquisa Médica Translacional/tendências , Receptores Proteína Tirosina Quinases/análise , Testes Imunológicos/tendências , Células Estromais , Células Estromais/patologia
16.
Trauma (Majadahonda) ; 25(4): 237-244, oct.-dic. 2014. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-132826

RESUMO

Objetivo: Estudiar si las células madre adultas mesenquimales en combinación con beta-fosfato tricálcico contribuyen significativamente a la regeneración en una lesión ósea traumatizada. Material y método: Se utilizó un modelo de defecto traumático en el fémur de ratas Wistar (n=39). Fueron diseñados dos grupos: el grupo 1 (control), con nueve ratas a las que se les realizó un defecto óseo vacío; y el grupo 2 (experimental), con tres subgrupos de 10 ratas cada uno, a las que se les administró beta-fosfato tricálcico (β-TCP) y β-TCP en combinación con células madre mesenquimales (MSC). Se valoraron resultados a las tres, seis y nueve semanas de evolución, respectivamente. Resultados: Se observó el 65% de las fracturas en los fémures derechos de las ratas, que en grupo experimental corresponde a la zona donde se implanta el carrie en combinación con las MSC, aunque sólo el 35% de éstas coinciden exactamente con esta zona. No existen diferencias de comportamiento biológico significativas, aunque se demuestra actividad osteogénica. Conclusión: El uso de β-TCP en combinación con células estromales mesenquimales no genera resultados significativos en la regeneración ósea. Todavía es necesario potenciar la investigación en fase experimental (AU)


Objective: To study whether adult mesenchymal stem cells in combination with a carrier of beta-tricalcium phosphate contributes significantly in regenerating bone injury in traumatized. Material and method: A traumatic defect model on the femur was used in Wistar rats (n=38). Two groups were designed: the group 1 (control) with 9 rats to which they there was realized a bone defect empty fault, and the group 2 (experimental), with three subgroups (2a, 2b, 2c) of 10 rat each, which were administered the carrier beta-tricalcium phosphate (β-TCP), and β-TCP in combination with mesenchymal stem cells (MSC). Results were valued to 3, 6 and 9 weeks of evolution, respectively. Results: There were 65% of the fractures in the right femur of rats in the experimental group that corresponds to the area where the carrier is implanted in combination with the MSC, but only 35% of these coincide exactly with this area. No significant differences in biological behavior, although it shows osteogenic activity. Conclusion: The use of β-TCP carrier in combination with mesenchymal stromal cells does not produce significant results in bone regeneration. There is a need for further research in experimental phase (AU)


Assuntos
Animais , Masculino , Feminino , Ratos , Regeneração Óssea , Regeneração Óssea/fisiologia , Células-Tronco/fisiologia , Fraturas do Fêmur/reabilitação , Fraturas do Fêmur/cirurgia , Fraturas do Fêmur/veterinária , Pesquisa com Células-Tronco , Modelos Animais , Fraturas do Fêmur/tratamento farmacológico , Fraturas do Fêmur , Células Estromais/citologia , Células Estromais
18.
Reumatol. clín. (Barc.) ; 10(1): 43-47, ene.-feb. 2014.
Artigo em Espanhol | IBECS | ID: ibc-120444

RESUMO

El calcio (Ca2+) es un catión con capacidad multifuncional como segundo mensajero en diferentes grupos celulares del sistema inmunitario que incluyen los linfocitos T y B, los macrófagos, los mastocitos, entre otras. Los recientes descubrimientos en relación con la entrada de Ca2+ dependiente de depósito (SOCE por su sigla en inglés, store operated calcium entry) han abierto nuevos caminos en la investigación de cómo este catión dirige el destino celular, en especial en los linfocitos T y B. La SOCE actúa a través de canales CRAC (del inglés Ca2+ release-activated Ca2+ channels) y su mecanismo de activación depende de la interacción de dos moléculas reguladoras: un sensor del Ca2+ del retículo endoplásmico o molécula de interacción estromal (STIM-1, del inglés stromal interaction molecule) y una subunidad poro del canal CRAC (Orai1). Esta revisión se centra principalmente en las funciones del Ca2+ en los linfocitos B y T, así como las alteraciones de estas vías implicadas en el desarrollo de enfermedades autoinmunes (AU)


Calcium (Ca2+) is an important cation able to function as a second messenger in different cells of the immune system, particularly in B and T lymphocytes, macrophages and mastocytes, among others. Recent discoveries related to the entry of Ca2+ through the store-operated calcium entry (SOCE) has opened a new investigation area about the cell destiny regulated by Ca2+ especially in B and T lymphocytes. SOCE acts through calcium-release-activated calcium (CRAC) channels. The function of CRAC depends of two recently discovered regulators: the Ca2+ sensor in the endoplasmic reticulum or stromal interaction molecule (STIM-1) and one subunit of CRAC channels called Orai1. This review focuses on the role of Ca2+ signals in B and T lymphocytes functions, the signalling pathways leading to Ca2+ influx, and the relationship between Ca2+ signals and autoimmune diseases (AU)


Assuntos
Humanos , Masculino , Feminino , Autoimunidade , Autoimunidade/imunologia , Autoimunidade/fisiologia , Células Estromais/patologia , Cálcio , Cálcio/isolamento & purificação , Sinalização do Cálcio/imunologia , Sinalização do Cálcio/fisiologia , Doenças Autoimunes/epidemiologia , Doenças Autoimunes/prevenção & controle , Espaço Intracelular/fisiologia , Espaço Intracelular
19.
Trauma (Majadahonda) ; 24(4): 230-238, oct.-dic. 2013. ilus
Artigo em Espanhol | IBECS | ID: ibc-118634

RESUMO

Objetivo: Estudiar el efecto terapéutico de la administración de células madre mesenquimales (CMM) para tratar secuelas neurológicas crónicas producidas tras una lesión cerebral. Material y método: En 20 ratas Wistar adultas y dos meses después de una lesión cerebral traumática, se realizó la administración subaracnoidea de CMM o suero. Se valoró la respuesta funcional de los animales y se hizo un análisis histológico del tejido cerebral. Resultados: No se obtuvieron mejoras significativas en la respuesta funcional de animales con CMM. Se localizaron CMM dentro del tejido cerebral, obteniéndose diferencias estadísticamente significativas en el tamaño de la lesión pero no en la expresión de marcadores neurales. Conclusión: El tratamiento subaracnoideo con CMM no tiene efecto en la respuesta neurológica de animales lesionados, aunque las células migraron y se integraron en el tejido cerebral, afectando a la morfología del mismo (AU)


Objective: To study the therapeutic effect of administration of mesenchymal stem cells (MSCs) to treat chronic neurological sequelae produced after traumatic brain injury (TBI). Material and method: In 20 adult Wistar rats and two months after TBI, subarachnoid administration of CMM or saline were performed. Functional response of the animals was assessed and a histological analysis of brain tissue was conducted. Results: There was no significant improvement in the functional response in animals with MSCs. MSCs were located within the brain tissue obtained statistically significant difference in lesion size but not in the expression of neural markers. Conclusion: Subarachnoid treatment with MSCs has no effect on neurological response of injured animals, although the cells migrated and integrated into the brain tissue affecting their morphology (AU)


Assuntos
Animais , Masculino , Feminino , Ratos , Lesão Encefálica Crônica/complicações , Lesão Encefálica Crônica/diagnóstico , Lesão Encefálica Crônica/cirurgia , Pesquisa com Células-Tronco , Células Estromais/patologia , Células Estromais/metabolismo , Células-Tronco/metabolismo , Transplante/tendências , Transplante
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