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1.
Endodoncia (Madr.) ; 37(2): 22-28, sept. 2019. graf, ilus
Artigo em Espanhol | IBECS | ID: ibc-186296

RESUMO

Objetivos. El propósito de este trabajo fue evaluar los efectos biológicos de MTA Repair HP y ProRoot MTA en células madre procedentes de ligamento periodontal (hPDLSCs) tras ser ex-puestos los cementos a ambiente ácido y neutro. Material y Métodos: Discos de cada material (n=30) fueron ex-puestos a un tampón fosfato-salino (pH 7.4) o a ácido butírico (pH 5.2) durante 7 días, posteriormente se realizaron pruebas biológicas in vitro usando hPDLSCs. A partir de eluatos de los diferentes materiales de obturación a retro, se analizaron pruebas de viabilidad celular y apoptosis. Para evaluar la adhesión celular, hPDLSCs se sembraron directamente sobre la superficie de los materiales y se observaron bajo microscopio electrónico de barrido. Para analizar estadísticamente los resultados se usaron los test ANOVA y Tukey test (p < 0.05). Resultados: Los cementos endodónticos expuestos a ambiente ácido mostraron un similar grado de adhesión celular, y sorprendentemente, MTA Repair HP a pH 5.2 exhibió una mayor viabilidad celular que ProRootMTA (p<0.05). A pH 7.4, ProRooT MTA obtuvo una tasa mayor de viabilidad celular que con MTA Repair HP. Conclusiones: Los materiales ProRoot MTA y MTA Repair HP presentaron adecuadas propiedades biológicas en los diferentes ambientes, en términos de viabilidad celular, apoptosis y adhesión


Objectives: The purpose of this work was to evaluate the biolo-gical effects of MTA Repair HP and ProRoot MTA on stem cells from periodontal ligament (hPDLSCs) after exposure to acidic and neutral environments. Material and Methods: Discs of each material (n=30) were ex-posed to phosphate buffered saline (pH = 7.4) or butyric acid (pH = 5.2) for 7 days, and biological testing was carried out in vitro on hPDLSCs. Cell viability and apoptosis assays were performed using eluates of each root-end filling material. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. Statistical differences were assessed by ANOVA and Tukey test (p < 0.05).Results: Endodontic cements exposure to an acidic environment showed a similar degree of cell adherence, and, surprisingly, MTA Repair HP exhibited higher cell viability rates at pH 5.2 than Pro-Root MTA, whereas ProRoot MTA 7.4 showed higher cell viability rates than MTA Repair HP. Conclusions: Adequate biological properties of ProRoot MTA and MTA Repair HP in terms of cell viability, cell death and cell attach-ment were observed in both environments


Assuntos
Humanos , Adolescente , Adulto Jovem , Adulto , Cimentos Dentários/uso terapêutico , Concentração de Íons de Hidrogênio , Ligamento Periodontal , Células-Tronco , Reações Biológicas , Separação Celular , Sobrevivência Celular , Apoptose , Materiais Restauradores do Canal Radicular/classificação , Materiais Restauradores do Canal Radicular/uso terapêutico , Obturação do Canal Radicular/métodos
2.
Med. oral patol. oral cir. bucal (Internet) ; 24(4): e529-e536, jul. 2019. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-185667

RESUMO

Background: Bisphosphonate-related osteonecrosis of the jaw is a pathological condition without effective established treatment and preventive strategies. The aim of this study was to analyse the effect of adipose-derived stem cells (ASC) in an experimental murine model of osteonecrosis. Material and Methods: 38 Wistar rats were injected intraperitoneally with zoledronic acid. After treatment, upper jaw molars were extracted. The animals were randomly assigned to one of two groups. In the control group, saline solution was applied over the alveolar sockets after the tooth extractions. In the treatment group, ASCs were applied instead of saline solution. The control and treatment groups were subdivided based on the time of euthanasia. A clinical and histological analysis was performed. Results: The presence of osteonecrosis in alveolar bone was observed in a similar distribution in both groups. In the ASC-treated group, new bone formation was greater than in controls. Conclusions: In this study, application of ASCs showed greater new bone formation in an osteonecrosis-like murine model. Previous inhibited post-extraction bone remodelling could be reactivated, and these findings appeared to be secondary to implantation of ASCs


No disponible


Assuntos
Animais , Camundongos , Ratos , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos , Conservadores da Densidade Óssea , Osteonecrose , Difosfonatos , Modelos Animais de Doenças , Imidazóis , Células-Tronco , Extração Dentária , Ratos Wistar
3.
Cir. plást. ibero-latinoam ; 45(2): 107-114, abr.-jun. 2019. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-184218

RESUMO

Introducción y objetivo. Las células madre son candidatas terapéuticas para una amplia gama de enfermedades. Resultan de gran interés en Cirugía Plástica para el tratamiento de heridas crónicas, transferencia de tejido adiposo y colgajos. El objetivo de este estudio es describir el método de aislamiento, cultivo y caracterización de células madre derivadas de tejido adiposo y cómo estas pueden precondicionarse con hipoxia y generar cambios in vitro en su capacidad proliferativa y migratoria. El trabajo es un complemento didáctico a otro publicado por nosotros en esta misma revista utilizando esta metodología en comparación al grupo de retardo de colgajo y grupo control en colgajos cutáneos aleatorizados en ratas. Métodos. Obtuvimos las células madre de tejido graso ínguino-abdominal de ratas adultas: 10 en el grupo de células madre derivadas de tejido adiposo y otras 10 en el grupo de células madre derivadas de tejido adiposo precondicionadas con hipoxia (2% O2 y 5% CO2). Realizamos análisis morfológico directo y con inmunofluorescencia con el marcador vimentina y CD90 y estudio de proliferación y migración celular in vitro. Resultados: Utilizamos en promedio 1.64 +/- 1.13 gr de tejido adiposo en el grupo sin precondicionamiento y 0.93 +/- 0.34 gr en el grupo con precondicionamiento con hipoxia para el aislamiento. Las células madre derivadas de tejido adiposo precondicionadas con hipoxia presentaron un aumento de la capacidad migratoria a las 24 horas de 2.44 +/- 0.85 mm frente a 2.24 +/- 0.82 mm (p ≤ 0.01) y proliferativa 5.42 x105 +/- 1.03 x105 céls/ml frente a 3.26 x105 +/- 8.61 x104 céls/ml) (p ≤ 0.001) de forma significativa en comparación a las sin precondicionamiento. Conclusiones. Describimos en detalle un método de precondicionamiento de células madre mediante hipoxia. Logramos potenciar el efecto de las células madre aumentando en forma significativa su capacidad migratoria y proliferativa de forma precoz


Background and objective. Stem cells are therapeutic candidates for a wide range of diseases. They are of great interest in Plastic Surgery for the management of chronic wounds, adipose tissue transfer and flaps. The objective of this study is to describe the method of isolation, culture and characterization of stem cells derived from adipose tissue and how these can be preconditioned with hypoxia and generate in vitro changes in their proliferative and migratory capacity. This study is a didactic supplement to a paper published by us in this same journal using this methodology in comparison to the group of flap delay and control group in skin flaps randomized in rats. Methods. Stem cells were obtained from inguinal-abdominal fatty tissue of adult rats: 10 in the group of stem cells derived from adipose tissue and another 10 in the group of stem cells derived from adipose tissue preconditioned with hypoxia (2% O2 and 5% CO2). Direct morphological analysis was carried out and with immunofluorescence (vimentin and CD90 marker). Study proliferation and in vitro cell migration was performed. Results. An average of 1.64 +/- 1.13 gr of adipose tissue of the inguinoabdominal area was used in the group of stem cells without preconditioning and 0.93 +/- 0.34 gr. in the group with hypoxic preconditioning for the isolation. Stem cells derived from adipose tissue preconditioned with hypoxia showed an increase in migratory capacity at 24 hours of 2.44 +/- 0.85 mm v/s 2.24 +/- 0.82 mm (p ≤ 0.01) and proliferative of 5.42 x105 +/- 1.03 x105 cells / ml v/s 3.26 x 105 +/- 8.61 x104 cells / ml) (p ≤0.001) significantly compared to those without preconditioning. Conclusions. A method of preconditioning stem cells by hypoxia is described in detail. It is possible to enhance the effect of the stem cells, significantly increasing their early migratory and proliferative capacity


Assuntos
Animais , Masculino , Ratos , Células-Tronco/citologia , Tecido Adiposo/citologia , Hipóxia/veterinária , Tecido Adiposo/enzimologia , Movimento Celular , Ratos Sprague-Dawley , Técnicas In Vitro/instrumentação , Tecido Adiposo/metabolismo , Imuno-Histoquímica , Proliferação de Células , Imunofluorescência , Neovascularização Fisiológica
4.
Cient. dent. (Ed. impr.) ; 16(1): 47-54, ene.-abr. 2019. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-183381

RESUMO

Cuando se combinan entre sí, las células madre, las matrices y los factores de crecimiento tienen la capacidad de regenerar un tejido, tal como el complejo dentino-pulpar. La ingeniería tisular está adquiriendo cada vez más importancia en el campo de la endodoncia como una alternativa a la obturación clásica con gutapercha. El objetivo de esta revisión es comparar los diferentes materiales que actuarían como matrices disponibles en la actualidad, así como conocer el alcance del conocimiento sobre los factores de crecimiento relacionados con la regeneración de la pulpa, señalando las principales limitaciones y elaborando un protocolo para futuros procedimientos regenerativos. Las matrices de colágeno han demostrado ser una opción óptima, ya que son biodegradables, pueden ser autólogos y existe una alta disposición. El factor de crecimiento insulínico tipo 1 y el factor de crecimiento derivado de plaquetas han demostrado estar implicados en la proliferación celular. El factor de crecimiento endotelial vascular es uno de los más importantes para la proliferación de la red vascular. El aislamiento celular y los altos costos son los principales obstáculos. La combinación de un andamio de colágeno, factor de crecimiento insulínico tipo 1, factor de crecimiento derivado de plaquetas, factor de crecimiento endotelial vascular y células madre de tejido pulpar de dientes deciduos (SHEDs) podría dar buenos resultados en la regeneración de la pulpa dental


When combined together, stem cells, scaffolds and growth factors have the ability of regenerating a whole tissue, for example the dental-pulp complex. Tissue engineering is getting more and more importance in the endodontic field as an alternative to the standard filling of root canals with gutta-percha material. The aim of this review is to compare the different scaffold materials available today, find out the extent of knowledge about the growth factors related to pulp regeneration, pointing out the main limitations and devise a protocol for future regenerative procedures. Collagen scaffolds material have shown to be an optimal choice as they are biodegradable, can be autologous and are highly available. Insulin-like growth factor 1 and platelet derived growth factor have shown to be involved in cells proliferation. Vascular endothelial growth factor is one of the most important for vascular network proliferation. Cells isolation and high costs are the main obstacles. As a conclusion, the combination of a collagen scaffold, insulin-like growth factor 1, platelet derived growth factor, vascular endothelial growth factor and dental pulp stem cells from deciduous teeth (SHEDs) might give good outcomes in dental pulp regeneration


Assuntos
Humanos , Engenharia Tecidual/métodos , Endodontia Regenerativa/métodos , Transplante de Células-Tronco , Tecido Periapical/citologia , Regeneração/fisiologia , Células-Tronco/fisiologia
5.
Actas urol. esp ; 42(7): 435-441, sept. 2018. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-174748

RESUMO

Contexto y objetivo: La reconstrucción vesical es el procedimiento para sustituir o ampliar la vejiga, siendo el intestino el tejido utilizado en la práctica clínica habitual. Las complicaciones de su uso van desde las propias de una resección intestinal hasta las resultantes del contacto continuo de la orina con un tejido no preparado para ello. En este artículo se describen y clasifican los diferentes biomateriales y cultivos celulares utilizados en la ingeniería tisular vesical y se revisan los estudios realizados en humanos. Adquisición de la evidencia: Se ha realizado una revisión de la literatura publicada en la base Pubmed entre 1950 y 2017, siguiendo los principios de la declaración PRISMA. Síntesis de la evidencia: Se han realizado múltiples estudios in vitro y en modelo animal, pero solo se han realizado 18 experimentos en humanos, con un total de 169 pacientes. La pruebas actuales indican que utilizar una matriz acelular o bien un polímero sintético y adherirle in vitro células uroteliales y musculares lisas autógenas, o bien células madre, sería la aproximación más realista para realizar una reconstrucción vesical experimental. Conclusiones: La sustitución o ampliación vesical sin utilizar intestino continúa siendo hoy un reto, a pesar del progreso en la fabricación de biomateriales y del desarrollo de la terapia celular. Para plantear una traslación clínica efectiva será necesario en el futuro realizar estudios bien diseñados, con mayor número de pacientes y tiempo de seguimiento, además de estandarizar las pruebas funcionales de control


Context and objective: Bladder reconstruction is performed to replace or expand the bladder. The intestine is used in standard clinical practice for tissue in this procedure. The complications of bladder reconstruction range from those of intestinal resection to those resulting from the continuous contact of urine with tissue not prepared for this contact. In this article, we describe and classify the various biomaterials and cell cultures used in bladder tissue engineering and reviews the studies performed with humans. Acquisition of evidence: We conducted a review of literature published in the PubMed database between 1950 and 2017, following the principles of the PRISM declaration. Synthesis of the evidence: Numerous in vitro and animal model studies have been conducted, but only 18 experiments have been performed with humans, with a total of 169 patients. The current evidence suggests that an acellular matrix, a synthetic polymer with urothelial and autologous smooth muscle cells attached in vitro or stem cells would be the most practical approach for experimental bladder reconstruction. Conclusions: Bladder replacement or expansion without using intestinal tissue is still a challenge, despite progress in the manufacture of biomaterials and the development of cell therapy. Well-designed studies with large numbers of patients and long follow-up times are needed to establish an effective clinical translation and standardisation of the check-up functional tests


Assuntos
Humanos , Engenharia Tecidual/métodos , Derivação Urinária/métodos , Medicina Regenerativa/métodos , Bexiga Urinária/cirurgia , Terapia Baseada em Transplante de Células e Tecidos , Materiais Biocompatíveis , Medicina Regenerativa/instrumentação , Medicina Regenerativa/normas , Bexiga Urinária/patologia , Procedimentos Cirúrgicos Urológicos , Células-Tronco
6.
Med. oral patol. oral cir. bucal (Internet) ; 23(5): e569-e578, sept. 2018. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-176376

RESUMO

Background: Aggregatibacter-actinomycetemcomitans (A.actinomycetemcomitans) are strongly associated with localized-aggressive-periodontitis (LAgP). The study's aim was to test for the first time the effect of total sonicated A.actinomycetemcomitans-bacterial-fragments on gingival mesenchymal stem/progenitor cells' (G-MSCs) proliferation and regenerative gene expression in-vitro. Material and Methods: G-MSCs were isolated, characterized, expanded and stimulated by total sonicated A.actinomycetemcomitans-bacterial-fragments (0 (negative-control), 15, 60, 120 and 240μg/ml; serovar-b; n=6/group). Cellular proliferation and NF-κβ (NFKB1), Alkaline Phosphatase (ALPL), Collagen-I (COL1A1), Collagen-III (COL3A1), Osteonectin (SPARC) and Osteopontin (SPP1) m-RNA expression were assessed via reverse-transcription-polymerase-chain-reaction (RT-PCR) at 24, 48 and 72 hours and CFUs-ability evaluated at twelve days. Results: G-MSCs demonstrated stem/progenitor cells' characteristics. A.actinomycetemcomitans-bacterial-fragments (up to 72 hours) resulted in marked G-MSCs' proliferation over-time (p<0.001) and elevated NFKB1 (p= 0.017), COL1A1 (p=0.025), SPARC (p=0.025), decreased ALPL (p=0.017), with no significant differences for COL3A1 and SPP1 expression or stimulation times (p>0.05; Friedman-test). Longer-term stimulation for twelve days reduced G-MSCs' CFUs. Conclusions: Sonicated A.actinomycetemcomitans-bacterial-fragments' exert beneficial short-term effects on G-MSCs' proliferative and non-mineralized tissue forming aptitude. Results shed new light on the importance of periodontal treatment for LAgP patients, using power driven sonic/ultrasonic devices, which, in addition to reducing the subgingival microbial load, produces cell-stimulatory A.actinomycetemcomitans-bacterial-fragments, with positive attributes on tissue reparative/regenerative responses of tissue resident stem/progenitor cells in their niche


No disponible


Assuntos
Humanos , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Infecções por Pasteurellaceae/patologia , Células-Tronco/microbiologia , Gengiva/microbiologia , Periodontite/microbiologia , Periodontite/patologia , Proliferação de Células , Fatores Estimuladores de Colônias
7.
Cir. plást. ibero-latinoam ; 44(3): 259-268, jul.-sept. 2018. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-180024

RESUMO

Introducción y Objetivo: Realizar una clasificación de necrosis en colgajos cutáneos aleatorios (predispuesto a necrosis) según los hallazgos macroscópicos e histopatológicos, con o sin tratamiento para aumentar la supervivencia del colgajo, podría ser útil para analizar de forma global el colgajo y evaluar distintas modalidades para disminuir la necrosis. Nuestro objetivo es evaluar si existe más de un patrón de necrosis cutánea del colgajo cutáneo aleatorio al séptimo día de postoperatorio realizando una clasificación de necrosis y comparar la efectividad de los métodos de retardo del colgajo y células madres derivadas de tejido adiposo (ASCs) con y sin precondicionamiento para aumentar la supervivencia del colgajo. Material y Método: Utilizamos 40 ratas macho adultas sometidas a colgajo cutáneo aleatorio en el área dorsal de 2 x 8 cm agrupadas en control, RTC (retardo del colgajo), ASCs y ASCs-H (células madre derivadas de tejido adiposo precondicionadas con hipoxia). Realizamos cálculo del área, puntuación de necrosis y estudio histológico de los colgajos al séptimo día con hematoxilina-eosina, inmunohistoquímica con VEGF (factor de crecimiento endotelial vascular) y cuantificación del colágeno tipo I y III. Resultados: Según la clasificación, el tipo 0 fue considerado como piel normal; tipo 0+ con cambio adaptativo reversible; tipo 1 necrosis menor con inflamación superficial; tipo 2 necrosis menor con inflamación profunda; y finalmente el tipo 3 necrosis en su grado máximo. La piel sana mantiene la coloración y textura elástica y blanda. A medida que progresa la necrosis cambia hacia un tono más oscuro, y la textura y consistencia van aumentando hasta tornarse acartonada y delgada. En los tipos 0+ y 1 observamos focos de reepitelización temprana al séptimo día, mientras que en estadios más avanzados la necrosis fue completa. El grupo de RTC presenta una menor puntuación y por lo tanto necrosis de menor grado respecto al resto de los grupos tratados. Por otra parte, el porcentaje global de necrosis fue menor en los grupos tratados con ASCs y ASCs-H respecto al grupo control (p ≤ 0.05). Conclusiones: La clasificación y puntuación de necrosis parece ser un método adecuado para comprender la evolución de la necrosis del colgajo cutáneo aleatorio y como herramienta para investigación. La necrosis de bajo grado permitiría un afrontamiento conservador y expectante respecto a la de mayor grado. El grupo RTC presentó un puntuación menor al séptimo día con mayor capacidad de reepitelización, mientras que los tratamientos con ASCs y ASCs-H lograron una menor área de necrosis de forma global al séptimo día


Background and Objective: Perform a characterization and classification of necrosis in random skin flaps (predisposed to necrosis) according to the macroscopic and histopathological findings, with or without treatment to increase the survival of the flap, it could be useful to analyze in a global way the flap and evaluate different modalities for decrease necrosis. Our aim is to evaluate if there is more than one skin cutaneous necrosis pattern of the random cutaneous flap on the seventh postoperative day, performing a necrosis classification and to compare the effectiveness of the flap delay and adipose tissue derived stem cells (ASCs) with and without preconditioning methods to increase the survival of the flap. Methods: Forty adult male rats subjected to random skin flap in the dorsal area of 2 x 8 cm grouped into control group, RTC (flap delay), ASCs (adipose-derived stem cells) and ASCs-H (hypoxic preconditioning of adipose-derived stem cells), were used. Area calculation, necrosis score and histological study of flaps on the seventh day with hematoxylin-eosin, immunohistochemistry with VEGF (vascular endothelial growth factor) and quantification of type I and III collagen were performed. Results: According to the classification of necrosis, type 0 was considered normal skin; type 0+ with reversible adaptive change; type 1 minor necrosis with superficial inflammation; type 2 minor necrosis with deep inflammation; and finally type 3 necrosis in its maximum degree. Healthy skin maintains coloring, elastic and soft texture. As necrosis progresses shifts towards a darker tone, texture and consistency increases until it becomes stiff and thin. In types 0+ and 1, foci of early reepithelialization was observed on the seventh day, while more advanced in the classification stages, necrosis is complete. The group of RTC presents a lower score and therefore lesser degree of necrosis with respect to the rest of the treated groups. Moreover the overall percentage of necrosis was lower in the groups treated with ASCs and ASCs-H in the control group (p ≤ 0.05). Conclusions: The classification and the score of necrosis seems to be a suitable method for understanding the evolution of skin flap necrosis and as a tool for research. Low-grade necrosis allows conservative and expectant confrontation with respect to higher grades. The RTC group presented a lower necrosis score on the seventh day with greater reepithelialization capacity, whereas treatment with ASCs and ASCs-H achieved a lower area of necrosis overall on the seventh day


Assuntos
Animais , Ratos , Retalho Miocutâneo/cirurgia , Retalho Miocutâneo/veterinária , Células-Tronco , Ratos Sprague-Dawley , Cirurgia Plástica/veterinária , Retalho Miocutâneo/classificação , Reepitelização , Imuno-Histoquímica
8.
An Real Acad Farm ; 84(2): 154-163, abr.-jun. 2018.
Artigo em Espanhol | IBECS | ID: ibc-178053

RESUMO

La mayor parte de los tumores están estructurados jerárquicamente, siendo la población de células madre del cáncer la encargada de iniciar y mantener el tumor. Estas células madre del cáncer, debido a sus similitudes con las células madre sanas, tienen capacidad de auto-renovación y diferenciación. Existe un balance altamente regulado que controla ambos procesos. De forma que la eliminación de la auto-renovación directamente o la inducción terminal de diferenciación producen la eliminación de las células madre del cáncer y, por lo tanto, la capacidad de mantenimiento y regeneración de la neoplasia. El desarrollo de dos plataformas distintas para buscar compuestos de pequeño tamaño bioactivos que induzcan la diferenciación terminal de las células madre del cáncer ha permitido identificar distintos fármacos con potencial acción neoplásica. Estas plataformas están basadas en cribados de alto rendimiento y alto contenido usando un sistema de células madre embrionarias transformadas y un análisis bioinformático in silico. Usando la leucemia mieloide aguda como modelo de tumor jerárquico, estos compuestos se estudiado ex vivo y in vivo y se han optimizado para su evaluación clínica. Estas plataformas de descubrimiento de fármacos se han validado como una nueva aproximación experimental para identificar nuevos fármacos con actividad diferenciadora anti-tumoral


Most of the tumors are hierarchically organized, with a cellular subpopulation of cancer stem cells in the apex. This population is responsible for the initiation and maintenance of the tumor. Due to their "stem cell"-like properties, cancer stem cells display self-renewal and differentiation potential, both properties are tightly regulated. Thus, direct inhibition of self-renewal or induction of terminal differentiation will eradicate cancer stem cells and, thus, the maintenance and regeneration capacity of the neoplasia will be eliminated. Two distinct drug discovery platforms were developed to identify small bioactive compounds that induce terminal differentiation of cancer stem cells. These platforms are based on highthroughput and high-content screenings using a transformed embryonic stem cell system and an in silico bioinformatics analysis. Using acute myeloid leukemia as a hierarchically organized tumor model, these compounds were studied ex vivo and in vivo and optimized for clinical settings. These platforms have been validated as a new experimental approach to identify differentiating-inducing drugs as anti-neoplastic compounds


Assuntos
Humanos , Preparações Farmacêuticas , Neoplasias/tratamento farmacológico , Células-Tronco , Sistema Hematopoético , Diferenciação Celular , Autorrenovação Celular , Camundongos Endogâmicos , Antígenos CD34 , ADP-Ribosil Ciclase 1
9.
Rev. clín. esp. (Ed. impr.) ; 218(4): 199-206, mayo 2018. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-174259

RESUMO

Los avances científicos y técnicos en el área biomédica y la medicina regenerativa han permitido el desarrollo de nuevos tratamientos, denominados «terapias avanzadas», que engloban la terapia celular, la génica y la ingeniería tisular. Los productos biológicos que pueden fabricarse a partir de estos elementos se clasifican desde el punto de vista de la Agencia Española del Medicamento y Productos Sanitarios en medicamentos de terapias avanzadas, productos derivados de la sangre y trasplantes. Esta revisión pretende aportar información científica y administrativa, de utilidad para el clínico, sobre el uso de estos recursos biológicos


Scientific and technical advances in the areas of biomedicine and regenerative medicine have enabled the development of new treatments known as "advanced therapies", which encompass cell therapy, genetics and tissue engineering. The biologic products that can be manufactured from these elements are classified from the standpoint of the Spanish Agency of Medication and Health Products in advanced drug therapies, blood products and transplants. This review seeks to provide scientific and administrative information for clinicians on the use of these biologic resources


Assuntos
Humanos , Terapia Baseada em Transplante de Células e Tecidos/tendências , Medicina Regenerativa/tendências , Células-Tronco , Transferência de Tecnologia , Aprovação de Drogas/legislação & jurisprudência , Engenharia Tecidual/tendências , Pesquisa com Células-Tronco , Terapia Baseada em Transplante de Células e Tecidos/normas , Controle de Qualidade
10.
An Real Acad Farm ; 84(1): 39-51, ene.-mar. 2018. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-178048

RESUMO

Una hernia incisional consiste en una protrusión de tejido a través de una cicatriz traumática o quirúrgica en la pared abdominal. El tratamiento de este y de otros tipos de hernias pasa frecuentemente por la implantación de una malla quirúrgica para reforzar el tejido debilitado. Sin embargo, a menudo se produce una respuesta inflamatoria exacerbada que desemboca en diferentes complicaciones, teniendo consecuencias graves para el paciente. Considerando el potencial inmunomodulador de las células madre mesenquimales (MSCs) y de sus exosomas (exo-MSCs), en este estudio planteamos que la administración de ambos productos terapéuticos, conjuntamente con los selladores de fibrina que se utilizan frecuentemente para la fijación de las mallas quirúrgicas, podría ejercer un efecto biológico y terapéutico que ayudara a controlar esa respuesta inflamatoria y mejorara, por tanto, el éxito del tratamiento con mallas quirúrgicas. Los resultados obtenidos en este estudio mostraron, en un modelo murino de hernia incisional, que los exo-MSCs reducen la infiltración de macrófagos inflamatorios M1 y que existe una predominancia de citoquinas relacionadas con la respuesta Th2, y con ello, con la polarización de macrófagos hacia un fenotipo M2 antiinflamatorio, en el tejido circundante a las mallas en las que se vehicularon MSCs o sus exosomas. Este estudio concluye que la fijación de mallas quirúrgicas con selladores de fibrina combinados con MSCs o exo-MSCs tendría un efecto beneficioso en el tratamiento de la hernia incisional, en términos de reducción de la respuesta inflamatoria y modulación de una reacción exacerbada frente a un cuerpo extraño


An incisional hernia constitutes a tissue protrusion through a traumatic or surgical scar in the abdominal wall. Frequently, the treatment of the incisional hernia, as well as other types of hernia, involves the implantation of a surgical mesh to reinforce the weakened tissue. However, an exacerbated inflammatory response is commonly developed after this implantation, having serious consequences for the patient. Considering the immunomodulatory potential of mesenchymal stem cells (MSCs) and their exosomes (exo-MSCs), in this study we proposed that the administration of these two therapeutic products, together with fibrin sealants that are frequently used to fix surgical meshes, could have a beneficial biological and therapeutic effect that could help to modulate the inflammatory response and improve the success of the surgical mesh implantation. The results obtained in this work showed, in a murine model of incisional hernia, that exo-MSCs reduce M1 inflammatory macrophages infiltration and that there is a predominance of Th2- related cytokines in the surrounding tissue of MSCs or exo-MSCs treated meshes, favoring the macrophage polarization towards a M2 anti-inflammatory phenotype. This study concludes that mesh fixation with fibrin sealants co-administered with MSCs or exo-MSCs would have a beneficiary effect on the treatment of incisional hernia in terms of reduction of the inflammatory response and modulation of the foreign body reaction


Assuntos
Animais , Camundongos , Células-Tronco , Exossomos , Hérnia/tratamento farmacológico , Telas Cirúrgicas , Proteínas Inflamatórias de Macrófagos/uso terapêutico , Medula Óssea , Citometria de Fluxo/métodos , Citometria de Fluxo/veterinária
11.
Arch. bronconeumol. (Ed. impr.) ; 54(1): 31-38, ene. 2018. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-170417

RESUMO

Lung biofabrication is a new tissue engineering and regenerative development aimed at providing organs for potential use in transplantation. Lung biofabrication is based on seeding cells into an acellular organ scaffold and on culturing them in an especial purpose bioreactor. The acellular lung scaffold is obtained by decellularizing a non-transplantable donor lung by means of conventional procedures based on application of physical, enzymatic and detergent agents. To avoid immune recipient's rejection of the transplanted bioengineered lung, autologous bone marrow/adipose tissue-derived mesenchymal stem cells, lung progenitor cells or induced pluripotent stem cells are used for biofabricating the bioengineered lung. The bioreactor applies circulatory perfusion and mechanical ventilation with physiological parameters to the lung during biofabrication. These physical stimuli to the organ are translated into the stem cell local microenvironment - e.g. shear stress and cyclic stretch - so that cells sense the physiological conditions in normally functioning mature lungs. After seminal proof of concept in a rodent model was published in 2010, the hypothesis that lungs can be biofabricated is accepted and intense research efforts are being devoted to the topic. The current experimental evidence obtained so far in animal tests and in ex vivo human bioengineered lungs suggests that the date of first clinical tests, although not immediate, is coming. Lung bioengineering is a disrupting concept that poses a challenge for improving our basic science knowledge and is also an opportunity for facilitating lung transplantation in future clinical translation (AU)


La biofabricación de pulmones es un nuevo desarrollo en ingeniería de tejidos y medicina regenerativa destinada a proporcionar órganos que se podrían usar para trasplante. Se basa en la siembra de células en un andamio acelular del órgano y su cultivo en un biorreactor específico. El andamio acelular se obtiene descelularizando un pulmón no trasplantable por medio de procedimientos convencionales basados en agentes físicos, enzimáticos y detergentes. Para evitar el rechazo inmunitario del receptor del pulmón una vez trasplantado, en la biofabricación se usan células madre autólogas mesenquimales derivadas de médula ósea/tejido adiposo, células progenitoras de pulmón o células madre pluripotentes inducidas. El biorreactor aplica perfusión circulatoria y ventilación mecánica con parámetros fisiológicos al pulmón durante el proceso. Estos estímulos físicos al órgano se traducen en el microambiente local de la célula madre -por ejemplo, tensión de cizallamiento y estiramiento cíclico- para que las células perciban las condiciones fisiológicas típicas en el pulmón maduro con funcionamiento normal. Tras la publicación en 2010 de la prueba de concepto en un modelo de roedor, se ha aceptado la hipótesis de que los pulmones pueden ser biofabricados y se están dedicando grandes esfuerzos de investigación a este tema. Las pruebas experimentales obtenidas hasta ahora en ensayos con animales y ex vivo en pulmones humanos biofabricados indican que la fecha de las primeras pruebas clínicas, aunque no inmediata, se va acercando. La bioingeniería pulmonar es un concepto disruptivo que nos desafía a mejorar nuestros conocimientos científicos básicos y da una oportunidad para facilitar el trasplante de pulmón en una futura traslación clínica (AU)


Assuntos
Humanos , Bioengenharia/tendências , Transplante de Pulmão/métodos , Medicina Regenerativa/métodos , Pneumopatias/cirurgia , Órgãos Artificiais , Células-Tronco/imunologia
13.
Clin. transl. oncol. (Print) ; 19(11): 1358-1374, nov. 2017. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-167117

RESUMO

Purpose. To explore the inhibitory effect and mechanism of MSCs on melanoma proliferation. Methods. The inhibitory effect of MSCs on melanoma A375 cells was detected by co-culture and conditioned medium (CM) experiments using MTT method. The cell cycle was analyzed by flow cytometry. Then, Western Blot experiment detected the expression of proteins related to NF-κB signaling in A375 cells. The expression of IL-1Ra in MSCs was proved by RT-PCR. The over-expression and silencing vector pcDNA3.1-EGFP-IL-1Ra and pGPH1-IL-1R were constructed and transfected into MSCs cells. After that, the changes of inhibitory effect and cell cycle from MSCs-S and MSCs-O CM on A375 cells were explored. The expression of proteins related to NF-κB signaling in A375 cells after MSCs-S or MSCs-O CM treatment was detected by Western Blot. MSCs, MSCs-S, or MSCs-O and A375 cells were co-injected into nude mice under the arms, the growth of tumor was observed, the frozen sections were made, and H&E staining of tumor tissue was performed. Results. The proliferation of A375 cells was inhibited and the cell cycle of A375 was arrested by MSCs. The expressions of cytokines related to NF-κB signaling were down-regulated. Over-expression and silence of Interleukin 1 receptor antagonist (IL-1Ra), specifically blocking activation of NF-κB signaling, indicated that inhibitory effect from MSCs was enhanced or weakened respectively, which suggested that IL-1Ra was involved in the inhibitory effect. In vivo, tumor initiation and growth were significantly inhibited when A375 cells were co-injected with MSCs into nude mice, which were related to the expression level of IL-1Ra. Conclusion. MSCs could inhibit the proliferation and tumor initiation of melanoma A375 cells through NF-κB signaling. MSCs could secret IL-1Ra and inhibit expressions of NF-κB signaling-related factors of tumor cells, and cause cell cycle arrest in G1 phase (AU)


No disponible


Assuntos
Humanos , Células-Tronco/patologia , Melanoma/diagnóstico , Proliferação de Células , Técnicas de Cultura de Células/métodos , Condrossarcoma Mesenquimal/diagnóstico , Western Blotting/métodos
14.
Clin. transl. oncol. (Print) ; 19(7): 907-914, jul. 2017. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-163446

RESUMO

Purpose. Biological effects of low-dose radiation (LDR) are distinguishable from those of high-dose radiation. Adaptive response is an important biological effect following low-dose radiation. Cancer stem cells (CSCs) have self-renewal and multidirectional differentiation potency which results in relapse and metastasis of cancer. In this study, we aimed to examine whether adaptive response could be induced in CSCs by LDR. Methods. Parental cells of three colon cancer cell lines (HRT18, HT29, and HCT116) and CSCs of these three cell lines were irradiated with LDR (i.e., D1) and then high-dose radiation (HDR) of X-rays (i.e., D1 + D2) or only HDR (D2 alone), followed by examination of adaptive response. Results. Adaptive response was not observed either in the three tumor parental cells lines or in three CSCs lines following LDR, due to the lack of resistance to subsequent D2-induced cell growth inhibition. Conclusion. These results suggested that LDR may not induce adaptive response in colon cancer cells or colon CSCs under in vitro conditions. Our study provided experimental and clinical foundations for the application of LDR in the treatment of colon cancers (AU)


No disponible


Assuntos
Humanos , Neoplasias do Colo/radioterapia , Células-Tronco/patologia , Células-Tronco/efeitos da radiação , Linhagem Celular/patologia , Linhagem Celular/efeitos da radiação , Radioterapia/métodos , Imunofluorescência/métodos , Relação Dose-Resposta à Radiação , Colo/citologia , Colo/patologia , Colo/efeitos da radiação
15.
Med. oral patol. oral cir. bucal (Internet) ; 22(3): e314-e323, 1 mayo 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-163198

RESUMO

Background: Primordial Odontogenic Tumor (POT) is a recently described odontogenic tumor characterized by a variably cellular loose fibrous tissue with areas similar to the dental papilla, covered by cuboidal to columnar epithelium that resembles the internal epithelium of the enamel organ, surrounded at least partly by a delicate fibrous capsule. The purpose of this study was to investigate the possible histogenesis and biological behavior of this rare tumor by means of a wide immunohistochemical analysis of its epithelial and mesenchymal components. Material and Methods: The immunoexpression of twenty-three different antibodies were evaluated in four cases of POT. Results: The epithelial cells that cover the periphery of the tumor showed immunopositivity for Cytokeratins 14 and 19, while Amelogenin, Glut-1, MOC-31, Caveolin-1. Galectin-3, PITX2, p53, Bax, Bcl-2, Survivin and PTEN were variably expressed in focal areas. The mesenchymal component of the tumor was positive for Vimentin, Syndecan-1, PITX2, Endoglin (CD105), CD 34, Cyclin D1, Bax, Bcl-2, Survivin and p53. PTEN and CD 90 showed a moderate positivity. BRAF V600E and Calretinin were negative in all samples. Cell proliferation markers (Ki-67, MCM-7) were expressed in < 5% of the tumor cells. Conclusions: According to these immunohistochemical findings, we may conclude that POT is a benign odontogenic tumor in which there is both epithelial and mesenchymal activity during its histogenesis, as there is expression of certain components in particular zones in both tissues that suggests this tumor develops during the immature (primordial) stage of tooth development, leading to its inclusion within the group of benign mixed epithelial and mesenchymal odontogenic tumours in the current World Health Organization classification of these lesions (AU)


No disponible


Assuntos
Humanos , Tumores Odontogênicos/patologia , Imuno-Histoquímica/métodos , Neoplasias Maxilares/patologia , Mesoderma/patologia , Células-Tronco/patologia , Epitélio/patologia
16.
Nefrología (Madr.) ; 37(2): 126-137, mar.-abr. 2017. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-162166

RESUMO

En la década de los 90 se descubrió un nuevo sistema de comunicación célula-célula, que consiste en la liberación de vesículas cargadas con partículas bioactivas (proteínas, mRNA, miRNA, metabolitos, etc.) en el espacio extracelular. Este tipo de comunicación se ha conservado durante la evolución, hecho que justificaría que la mayoría de los tipos celulares puedan generarlas. Estas vesículas extracelulares (VE) pueden regular diversos procesos fisiológicos, así como el desarrollo y progresión de enfermedades. En los últimos años se ha extendido el estudio de las VE generadas principalmente por células madre adultas o embrionarias, células sanguíneas, células del sistema inmune y nervioso, así como células tumorales. El análisis de VE en fluidos corporales ha sido utilizado como herramienta de diagnóstico en cáncer y recientemente para distintas enfermedades renales. Sin embargo, en esta revisión pretendemos analizar la importancia, función y posible aplicación clínica de las VE generadas por células madre en enfermedades renales y en trasplantes (AU)


A new cell-to-cell communication system was discovered in the 1990s, which involves the release of vesicles into the extracellular space. These vesicles shuttle bioactive particles, including proteins, mRNA, miRNA, metabolites, etc. This particular communication has been conserved throughout evolution, which explains why most cell types are capable of producing vesicles. Extracellular vesicles (EVs) are involved in the regulation of different physiological processes, as well as in the development and progression of several diseases. EVs have been widely studied over recent years, especially those produced by embryonic and adult stem cells, blood cells, immune system and nervous system cells, as well as tumour cells. EV analysis from bodily fluids has been used as a diagnostic tool for cancer and recently for different renal diseases. However, this review analyses the importance of EVs generated by stem cells, their function and possible clinical application in renal diseases and kidney transplantation (AU)


Assuntos
Humanos , Vesículas Extracelulares , Insuficiência Renal Crônica/terapia , Lesão Renal Aguda/terapia , Terapia Baseada em Transplante de Células e Tecidos/métodos , Comunicação Celular , Células-Tronco/fisiologia , Transplante de Rim
18.
Rev. osteoporos. metab. miner. (Internet) ; 9(1): 5-12, ene.-mar. 2017. tab, ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-162865

RESUMO

El tejido adiposo contiene un gran número de células madre mesenquimales (Adipose Stem Cells, ASCs) que residen en su estroma vascular. Aunque existe controversia acerca de la capacidad de generar tejido óseo de estas células in vivo, in vitro constituyen un buen modelo de diferenciación osteogénica debido a su semejanza fenotípica con las células estromales de la médula ósea (Bone Marrow Stromal Cells, BMSCs) en cultivo. La diferenciación de las poblaciones osteoprogenitoras de la médula ósea está intensamente regulada por factores locales, como el factor de crecimiento endotelial vascular (VEGF) y la proteína relacionada con la parathormona (PTHrP), que modulan la proliferación de estas poblaciones en distintos estadios de diferenciación. Tanto el VEGF como el fragmento N-terminal de la PTHrP ejercen efectos osteogénicos. En este estudio hipotetizamos que sus efectos sobre la proliferación celular de los osteoprogenitores son dependientes del estadio de diferenciación osteoblástica. Tras confirmar su capacidad de diferenciación in vitro por expresión génica de Runx2 y acumulación de calcio, se analizó la respuesta proliferativa a estímulos con VEGF o PTHrP(1-36) de ASCs sometidas o no a inducción osteogénica. VEGF pero no PTHrP(1-36) estimuló la capacidad proliferativa de las ASCs no inducidas mientras que PTHrP(1-36), pero no VEGF, estimuló la proliferación de las ASCs inducidas, corroborando el papel diferencial de estos factores de crecimiento en distintos estadios de diferenciación (AU)


Adipose tissue contains a large number of mesenchymal stem cells (ASCs) residing in their vascular stroma. Although there is controversy regarding the ability to generate bone tissue from these cells in vivo, the in vitro cells offer a good model of osteogenic differentiation due to its phenotypic similarity with the bone marrow stromal cells (BMSCs) in culture. The differentiation of osteo-progenitor populations of bone marrow is intensely regulated by local factors, such as vascular endothelial growth factor (VEGF) and parathyroid hormone-related protein (PTHrP), which modulate these populations' proliferation in different stages of differentiation. Both the VEGF and the N-terminal fragment of the PTHrP exert osteogenic effects. In this study, we posited that its effects on proliferation of osteo-progenitors are stage dependent of osteoblastic differentiation. After confirming its capacity to in vitro differentiation by Runx2 gene expression and accumulation of calcium, the proliferative response to stimuli was analyzed with VEGF or PTHrP (1-36) of ASCs submitted or not to osteogenic induction. VEGF, but not PTHrP (1- 36), stimulated the proliferative capacity of uninduced ASCs, whereas BMSCs, but not VEGF, stimulated the proliferation of induced ASCs, corroborating the differential role of this growth in different stages of differentiation (AU)


Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Fatores de Crescimento do Endotélio Vascular/análise , Células-Tronco/metabolismo , Tecido Adiposo/cirurgia , Tecido Adiposo/metabolismo , Medula Óssea/metabolismo , Anticorpos Monoclonais Murinos/análise , Citometria de Fluxo , Antígenos de Diferenciação/análise , Antígenos CD/análise , Proliferação de Células , Reação em Cadeia da Polimerase
19.
Rev. clín. esp. (Ed. impr.) ; 217(1): 30-34, ene.-feb. 2017. ilus
Artigo em Espanhol | IBECS | ID: ibc-159530

RESUMO

Al cumplirse 10 años del descubrimiento de las células pluripotenciales inducidas se revisan los principales resultados en sus distintos campos de aplicación, los obstáculos con los que se ha encontrado su experimentación, así como las posibles aplicaciones en la práctica clínica. La eficacia de las células pluripotenciales inducidas en la experimentación clínica puede equipararse a la de las células troncales embrionarias humanas, pero, a diferencia de estas, no presentan la grave dificultad ética que conlleva la necesidad de destruir embriones humanos para su obtención. El hallazgo de estas células, que constituyó en su día un verdadero hito científico merecedor de un Premio Nobel de Medicina, está hoy rodeado de luces y sombras: grandes esperanzas en la medicina regenerativa frente a riesgos aún no bien controlados de reacciones imprevisibles, tanto en los procesos de desdiferenciación como en la posterior diferenciación hacia las estirpes celulares empleadas con fines terapéuticos o de experimentación (AU)


On the 10-year anniversary of the discovery of induced pluripotent stem cells, we review the main results from their various fields of application, the obstacles encountered during experimentation and the potential applications in clinical practice. The efficacy of induced pluripotent cells in clinical experimentation can be equated to that of human embryonic stem cells; however, unlike stem cells, induced pluripotent cells do not involve the severe ethical difficulties entailed by the need to destroy human embryos to obtain them. The finding of these cells, which was in its day a true scientific milestone worthy of a Nobel Prize in Medicine, is currently enveloped by light and shadow: high hopes for regenerative medicine versus the, as of yet, poorly controlled risks of unpredictable reactions, both in the processes of dedifferentiation and subsequent differentiation to the cell strains employed for therapeutic or experimentation goals (AU)


Assuntos
Humanos , Masculino , Feminino , Células-Tronco Pluripotentes Induzidas/fisiologia , Células-Tronco/patologia , Células-Tronco/fisiologia , Medicina Regenerativa/história , Medicina Regenerativa/métodos , Reprogramação Celular/fisiologia , Medicina Regenerativa/organização & administração , Medicina Regenerativa/normas , Medicina Regenerativa/tendências , Reprogramação Celular/genética , Técnicas de Reprogramação Celular/instrumentação , Técnicas de Reprogramação Celular/normas
20.
Prog. obstet. ginecol. (Ed. impr.) ; 60(1): 16-23, ene.-feb. 2017. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-164028

RESUMO

Objetivo: valorar el estado actual del tratamiento de la incontinencia urinaria en estudios de experimentación animal y clínica. Revisión: en el mundo hay más de 200 millones de mujeres con incontinencia urinaria, circunstancia que limita la calidad de vida. La incontinencia urinaria de esfuerzo es el tipo de incontinencia más frecuente. En los últimos años se ha propuesto el tratamiento con células madre habiendo sido objeto de trabajos de experimentación animal y clínicos. Se realiza una revisión crítica de las ventajas e inconvenientes de la utilización de células autólogas procedentes de medula ósea, tejido graso, muscular y de cordón umbilical. Se valoran las vías de administración y la metodología utilizada, proponiendo nuevas formas de administración y de los trabajos clínicos, mecanismos de acción y potenciales efectos secundarios. Por último se analizan los dispares resultados clínicos que oscilan entre el 88,9% y el 13,2%. Conclusión: el tratamiento de la incontinencia urinaria con células madre en el futuro podría colaborar a mejorar la calidad de vida de estas pacientes (AU)


Objective: Study the actual status of the urinary incontinence treatment with stem cells on animal and clinical experience. Review: More than 200 million people worldwide, affected with urinary incontinence with reduced quality of life. Stress urinary incontinence has been reported as the most common type of urinary incontinence. Stem cell for the regenerative repair of the stress urinary incontinence has been proposed during the last years, many experimental studies on animal models and some in clinical. This is a critical review of advantages and disadvantages of autologous cells use from bone marrow, fat tissue, muscle and umbilical cord. Administration ways and procedures are described and methology of administration with new ways of treatments are proposed. Study designs are discussed in terms of action mechanisms and possible disadvantages. Finally, discordant results ranging from 88.9% to 13.2% improvement rates are discussed. Conclusion: Urinary stress incontinence treatment with stem cells in the future could improve the quality of life of this kind of patients (AU)


Assuntos
Animais , Células-Tronco/fisiologia , Incontinência Urinária por Estresse/terapia , Qualidade de Vida , Modelos Animais , Transplante de Células-Tronco , Biópsia , Células-Tronco/classificação , Incontinência Urinária/terapia , Mioblastos/citologia , Células da Medula Óssea/fisiologia , Estimulação Elétrica/métodos , Músculo Estriado/transplante , Músculo Estriado/citologia
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