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Med. oral patol. oral cir. bucal (Internet) ; 22(3): e314-e323, 1 mayo 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-163198


Background: Primordial Odontogenic Tumor (POT) is a recently described odontogenic tumor characterized by a variably cellular loose fibrous tissue with areas similar to the dental papilla, covered by cuboidal to columnar epithelium that resembles the internal epithelium of the enamel organ, surrounded at least partly by a delicate fibrous capsule. The purpose of this study was to investigate the possible histogenesis and biological behavior of this rare tumor by means of a wide immunohistochemical analysis of its epithelial and mesenchymal components. Material and Methods: The immunoexpression of twenty-three different antibodies were evaluated in four cases of POT. Results: The epithelial cells that cover the periphery of the tumor showed immunopositivity for Cytokeratins 14 and 19, while Amelogenin, Glut-1, MOC-31, Caveolin-1. Galectin-3, PITX2, p53, Bax, Bcl-2, Survivin and PTEN were variably expressed in focal areas. The mesenchymal component of the tumor was positive for Vimentin, Syndecan-1, PITX2, Endoglin (CD105), CD 34, Cyclin D1, Bax, Bcl-2, Survivin and p53. PTEN and CD 90 showed a moderate positivity. BRAF V600E and Calretinin were negative in all samples. Cell proliferation markers (Ki-67, MCM-7) were expressed in < 5% of the tumor cells. Conclusions: According to these immunohistochemical findings, we may conclude that POT is a benign odontogenic tumor in which there is both epithelial and mesenchymal activity during its histogenesis, as there is expression of certain components in particular zones in both tissues that suggests this tumor develops during the immature (primordial) stage of tooth development, leading to its inclusion within the group of benign mixed epithelial and mesenchymal odontogenic tumours in the current World Health Organization classification of these lesions (AU)

No disponible

Humanos , Tumores Odontogênicos/patologia , Imuno-Histoquímica/métodos , Neoplasias Maxilares/patologia , Mesoderma/patologia , Células-Tronco/patologia , Epitélio/patologia
Eur. j. anat ; 21(2): 97-112, abr. 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-163135


The gastrointestinal stromal tumour (GISTs), the most common mesenchymal neoplasm in the gastrointestinal tract, has been the subject of great interest in recent years in terms of prognosis, diagnosis and treatment. Its etiology is linked to the mutation of c-KIT and PDGFRA genes, although between 5 and 15% show no signs of such mutations. It is still diagnosed using immunohistochemical staining. The first line of treatment continues to be surgery, although advances in the molecular biology of GISTs are facilitating the development of new treatment strategies. Those that act by regulating tyrosine kinase activity are of particular interest. Drugs such as imatinib and sunitinib have improved the prognosis of these patients, although the development of resistance constitutes one of the main limitations of the treatment. The aim of this review is to present an up-to-date overview of the main etiopathogenic, diagnostic and therapeutic aspects of these tumours

No disponible

Humanos , Tumores do Estroma Gastrointestinal/patologia , Mesoderma/patologia , Neoplasias Gastrointestinais/patologia , Células Estromais/patologia , Nanotecnologia/métodos , Proteínas Proto-Oncogênicas c-kit/análise , Marcadores Genéticos
Av. odontoestomatol ; 32(2): 97-105, mar.-abr. 2016.
Artigo em Espanhol | IBECS | ID: ibc-152001


Desde la organogénesis y hasta estadios adultos, las células madre mesenquimales participan activamente dando origen y manteniendo la homeostasis del organismo. En la cavidad oral han sido aisladas desde variadas estructuras del órgano dental tales como el ligamento periodontal, pulpa dental, tejido gingival, folículo dental y papila apical significando una prometedora fuente de células madre mesenquimales las que pueden ser caracterizadas de acuerdo a los criterios mínimos establecidos por 'The International Society for Cellular Therapy' que son: a) La adherencia al plástico; b) La expresión de marcadores CD73, CD90, CD105 y la carencia de CD34,CD45, CD14, CD11, CD79, CD19 y HLA-DR (clase II); c) Capacidad multipotencial de diferenciación hacia linajeosteogénico, condrogénico y adipogénico. El objetivo de esta revisión consiste en realizar un levantamiento de la situación actual de este tema efectuando una revisión comprensiva de la literatura en los campos de; identificación a través de marcadores de superficie, aislamiento por medio de mecanismos de digestión enzimática o explante, almacenamiento atendiendo a la necesidad de suprimir el uso de suero fetal bovino como medio de cultivo en un esfuerzo por avanzar hacia aplicaciones terapéuticas, banca o criopreservación destacando nuevas experiencia en este campo como lo es la criopreservación de piezas dentales completas gracias a la tecnología láser Nd:YAG. Y, finalmente, las aplicaciones clínicas que promete este grupo de células a través de la medicina regenerativa y la ingeniería tisular tanto en el campo de la odontología como la medicina general (AU)

Since organogenesis and even adult stages, mesenchymal stem cells actively participate starting and maintaining body homeostasis. In the oral cavity they have been isolated from various structures dental organ such as the periodontal ligament, dental pulp, gingiva, dental follicle and apical papilla meaning a promising source of mesenchymal stem cells which can be characterized according to the minimum criteria set by The International Society for Cellular Therapy that are: a) the adherence to plastic, b) expression of markers CD73, CD90, CD105 and the absence of CD34, CD45, CD14, CD11, CD79, CD19 and HLA-DR (class II ) c) multipotent differentiation capacity to osteogenic lineage, chondrogenic and adipogenic. The objective of this review is to conduct a survey of the current status of this issue by a comprehensive review of the literature in the fields; identification through surface markers, isolation through mechanisms of enzymatic or explant, storage digestion with the need to eliminate the use of fetal bovine serum as the culture medium in an effort to move towards therapeutic applications, highlighting new banking and cryopreservation experience in this field such as the cryopreservation of whole teeth thanks to the NdYAG laser technology. And finally promising clinical applications this group of cells through regenerative medicine and tissue engineering both in the field of dentistry and general medicine (AU)

Humanos , Mesoderma/fisiologia , Células-Tronco , Medicina Regenerativa/métodos , Odontologia/tendências , Medicina Bucal/tendências , Terapia Baseada em Transplante de Células e Tecidos/tendências , Criopreservação , Engenharia Celular/tendências
Rev. esp. enferm. dig ; 107(10): 633-639, oct. 2015. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-141429


El tumor fibroso solitario (TFS) es una neoplasia mesenquimal infrecuente. Dado su origen, puede aparecer en prácticamente cualquier localización. En la literatura sólo hay 50 casos descritos de TFS localizado en el parénquima hepático. A pesar de su rareza, debe ser considerada dentro del diagnóstico diferencial de una masa hepática. Presentamos el primer caso con seguimiento por imagen desde 5 años antes del diagnóstico, tratado mediante embolización portal derecha y embolización arterial tumoral con posterior resección hepática, así como una revisión exhaustiva de los casos descritos hasta la actualidad (AU)

Solitary fibrous tumor (SFT) is a rare mesenchymal tumor. Given its origin, it can appear in almost any location. In the literature, only 50 cases of SFT in the liver parenchyma have been reported. Despite its rarity, this entity should be included in the differential diagnosis of liver masses. We report the first case with imaging data from five years prior to diagnosis, which was treated by right portal embolization and arterial tumor embolization, and subsequent liver resection. We also present an exhaustive review of the cases described to date (AU)

Idoso , Feminino , Humanos , Tumores Fibrosos Solitários/complicações , Tumores Fibrosos Solitários/cirurgia , Tumores Fibrosos Solitários , Embolização Terapêutica/instrumentação , Prognóstico , Tumores Fibrosos Solitários/fisiopatologia , Neoplasias Hepáticas/cirurgia , Neoplasias Hepáticas , Mesoderma/patologia , Mesoderma , Tomografia Computadorizada de Emissão/métodos , Tomografia Computadorizada de Emissão , Imuno-Histoquímica/métodos , Imuno-Histoquímica
Rev. esp. cir. ortop. traumatol. (Ed. impr.) ; 58(3): 138-143, mayo-jun. 2014. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-122523


Objetivo: Los miARN actúan como silenciadores génicos que están implicados en la regulación de funciones celulares esenciales. El miR-335 participa regulando los procesos de diferenciación celular en células progenitoras. Las células madre mesenquimales (MSC) son células progenitoras de los condrocitos y osteoblastos encargados del mantenimiento homeostático del cartílago y hueso. El objetivo de este estudio era determinar una posible asociación entre la expresión de miR-335 y la enfermedad artrósica. Metodología: Las MSC obtenidas de la médula ósea de 3 pacientes artrósicos y 3 controles sin signos clínicos de artrosis ni osteoporosis se cultivaron y caracterizaron fenotípica y funcionalmente en el pase 3 de cultivo. Así mismo, mediante PCR cuantitativa se determinaron los niveles de expresión de miR-335 y del gen mesoderm-specific transcript ---MEST---, que controla su expresión. Resultados: Se detectaron diferencias entre las MSC aisladas de pacientes con artrosis y los controles en los niveles de expresión de miR-335 y de MEST (mediana [rango intercuartílico]: 1,69 [0,85-1,74]; 3,85 [3,20-5,67]). Aunque las diferencias detectadas no alcanzaron una significación estadística (p = 0,1), sí se apreció una clara tendencia a una menor expresión de miR-335 en las MSC de pacientes artrósicos. Conclusiones: Teniendo en cuenta que miR-335 tiene como dianas potenciales diferentes genes que participan en la vía de se˜nalización de la Wnt, la tendencia observada podría determinar, al menos en parte, algunas de las alteraciones que determinan el inicio o progresión de la artrosis, y puede, por lo tanto, servir en el diseño de futuras dianas terapéuticas para el tratamiento de esta enfermedad (AU)

Objective: MiRNAs act as gene silencers that are involved in the regulation of essential cell functions. miR-335 is involved in regulating cell differentiation processes in progenitor cells. Mesenchymal stem cells (MSCs) are progenitor cells of chondrocytes and osteoblasts responsible for homeostatic maintenance of cartilage and bone. The aim of this study was to determine a possible relationship between the expression of miR-335 and osteoarthritis. Methods: MSCs obtained from the bone marrow of 3 osteoarthritic patients and 3 controls with no clinical signs of osteoarthritis or osteoporosis were cultured and phenotypically and functionally characterised in a 3-step culture. Expression levels of miR-335 and the mesodermspecific transcript gene ---MEST--- that controls its expression were determined by quantitative PCR. Results: Differences in the expression levels of miR-335 and MEST (median [interquartile range]: 1.69 [0.85-1.74], and 3.85 [3.20-5.67] were detected between MSCs isolated from patients with osteoarthritis and controls. Although the differences detected did not reach statistical significance (P = .1), a clear trend towards lower expression of miR-335 in osteoarthritis MSCs was observed. Conclusions: Given that miR-335 has the different genes involved in the Wnt signalling pathway as potential targets, the observed trend may help to ascertain, at least partially, some of the alterations which determine the onset or progression of osteoarthritis, and can therefore serve for the design of future therapeutic targets for the treatment of this disease (AU)

Humanos , MicroRNAs/fisiologia , Osteoartrite/fisiopatologia , Células-Tronco/fisiologia , Mesoderma/fisiologia , Inativação Gênica , Estudos de Casos e Controles , Transcrição Genética/fisiologia
Prog. obstet. ginecol. (Ed. impr.) ; 56(8): 418-423, oct. 2013. ilus
Artigo em Espanhol | IBECS | ID: ibc-115540


Los STUMPs (tumoraciones mesenquimales de músculo liso uterino de potencial incierto) son tumoraciones infrecuentes, que pertenecen a las tumoraciones mesenquimales de músculo liso uterino. Estas tumoraciones no pueden definirse ni como totalmente benignas ni malignas. Presentamos un caso clínico de una paciente de 27 años que consultó por sensación de plenitud, aumento del perímetro abdominal y polaquiuria de 6 meses de evolución. Ecográficamente se visualizó una masa intramural-subserosa de 9 cm en fondo uterino, con sospecha de degeneración hialina, que fue extirpada quirúrgicamente. El estudio anatomopatológico reveló el diagnóstico de tumoración muscular lisa de potencial maligno incierto, variante epiteloide. Conclusión. Los STUMPs se diagnostican anatomopatológicamente, siendo este proceso complejo y requiriendo, en gran número de ocasiones, técnicas inmunohistoquímicas para conseguir un diagnóstico y pronóstico más preciso. El tratamiento es quirúrgico, pudiendo realizar una histerectomía o bien, siendo más conservadores, la exéresis de la tumoración, ya que la mayoría tienen un comportamiento benigno (AU)

Uterine smooth-muscle tumours with unusual growth patterns represent an histologically heterogeneous and uncommon group of uterine smooth-muscle tumours that cannot be diagnosed as either benign or malignant.We report the case of a 27year-old woman who consulted for a 6-month history of bladder fullness sensation, abdominal distension and polaquiuria. Transvaginal ultrasound showed a 9cm intramural mass at the uterine fundus that was surgically excised. The anatomopathological diagnosis of uterine smooth-muscle tumour with growth pattern, epithelioid variant, was reported. Conclusions: The diagnosis of uterine smooth-muscle tumours may create great challenges for the pathologist who classifies these tumours by their histological features. The immunohistochemical staining may be helpful to establish their behaviour and prognosis. The clinical management remains a dilema and surgical treatment of choice has not been well defined, due to their uncertain clinical behaviour. Most of these tumours have a benign clinical course but these patients should receive long-term follow-up (AU)

Humanos , Feminino , Adulto , Músculo Liso/patologia , Músculo Liso/cirurgia , Músculo Liso , Leiomioma/cirurgia , Leiomioma , Neoplasias Uterinas/cirurgia , Neoplasias Uterinas , Mesoderma/patologia , Mesoderma/cirurgia , Mesoderma , Neoplasias do Colo do Útero/complicações , Neoplasias do Colo do Útero/cirurgia , Imuno-Histoquímica/métodos , Imuno-Histoquímica , Radiografia Torácica/métodos
Clin. transl. oncol. (Print) ; 14(4): 243-253, abr. 2012.
Artigo em Inglês | IBECS | ID: ibc-126184


Malignant mixed Müllerian tumours (malignant mixed mesodermal tumours, MMMT) of the uterus are metaplastic carcinomas with a sarcomatous component and thus they are also called carcinosarcomas. It has now been accepted that the sarcomatous component is derived from epithelial elements that have undergone metaplasia. The process that produces this metaplasia is epithelial to mesenchymal transition (EMT), which has recently been described as a neoplasia-associated programme shared with embryonic development and enabling neoplastic cells to move and metastasise. The ubiquitin proteasome system (UPS) regulates the turnover and functions of hundreds of cellular proteins. It plays important roles in EMT by being involved in the regulation of several pathways participating in the execution of this metastasis-associated programme. In this review the specifi c role of UPS in EMT of MMMT is discussed and therapeutic opportunities from UPS manipulations are proposed (AU)

Humanos , Masculino , Feminino , Carcinoma/metabolismo , Neoplasias do Endométrio/metabolismo , Transição Epitelial-Mesenquimal , Regulação Neoplásica da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Complexo de Endopeptidases do Proteassoma/química , Ubiquitinas/química , Adesão Celular , Citoplasma/metabolismo , Mesoderma/metabolismo , Modelos Biológicos , beta Catenina/metabolismo , Proteína Supressora de Tumor p53/metabolismo
Clin. transl. oncol. (Print) ; 12(3): 234-237, mar. 2010. ilus
Artigo em Inglês | IBECS | ID: ibc-124063


Hepatobiliary cystadenocarcinomas (BCACs) with mesenchymal stroma are a rare cystic lesion. This tumour needs to be distinguished from benign biliary cystadenoma, which is antecedent in most cases. The treatment of choice is radical excision of the mass. The diagnostic evaluation, surgical management, pathological characteristics, treatment and follow-up of one patient with hepatobiliary cystadenocarcinoma with ovarian stroma is described. Preoperative diagnosis of BCACs is often difficult, because their clinical manifestations are similar to those of other hepatic cystic lesions. MRI is suitable for accurate characterisation of cystic biliary lesions, but distinguishing between cystadenoma and cystadenocarcinoma remains difficult on the basis of imaging findings. Complete surgical excision gives a relatively good chance of long-term survival because of the slow growth rate of these tumours (AU)

No disponible

Humanos , Feminino , Adulto , Neoplasias dos Ductos Biliares/patologia , Neoplasias do Sistema Biliar/patologia , Cistadenocarcinoma/patologia , Mesoderma/patologia , Segunda Neoplasia Primária/patologia , Segunda Neoplasia Primária/cirurgia , Adenocarcinoma Papilar/patologia , Antineoplásicos/uso terapêutico , Neoplasias dos Ductos Biliares/cirurgia , Quimioterapia Adjuvante/métodos , Quimioterapia Adjuvante , Hepatectomia/métodos , Hepatite B/complicações , Neoplasias da Glândula Tireoide/patologia
Cir. plást. ibero-latinoam ; 35(2): 135-140, abr.-mayo 2009. ilus
Artigo em Espanhol | IBECS | ID: ibc-85478


Los defectos abdominales congénitos de la línea media inferior, como la extrofia cloacal, se producen por fallos en el mesodermo entre la región umbilical y la membrana cloacal provocando severos defectos viscerales, musculares y óseos. Los reiterados intentos para la reconstrucción de los tractos intestinal y génitourinario en este tipo de malformaciones, pueden ocasionar secuelas graves en la pared malformada. La complejidad de esta malformación y los numerosos procedimientos a los que deben ser sometidos estos pacientes, requieren de un abordaje interdisciplinario desde el inicio del tratamiento y en cada una de las etapas reconstructivas a fin de evitar, al máximo, las lesiones delos tejidos abdominales para lograr, al final, una pared adecuada. Presentamos 2 casos de reconstrucción de la pared abdominal en sendos pacientes de sexo femenino con secuelas importantes de extrofia cloacal, utilizando tejidos expandidos, colgajos musculares y complementando el tratamiento en una de las pacientes con una malla protésica. En ambos casos, y a pesar de la falta de tejido provocada por la malformación y las secuelas de múltiples cirugías, obtuvimos un buen resultado funcional y estético (AU)

Abdominal congenital defects of the middle line have their origin in developmental faults of mesoderm between the umbilical region and the cloacal membrane, originating visceral, muscular and osseous defects in the abdominal wall. Repeated attempts to reconstruct the intestinal and genitourinary tract here and in other malformations, can cause serious sequeals in the previously deformed abdominal wall. We present 2 cases of abdominal wall reconstruction inpatients with serious sequelae of cloacal extrophy. Complexity of this malformation calls for an interdisciplinary treatment to avoid the severe damage that may becaused during reconstructive attempts. In spite of lack of tissue because of the malformation and the sequelae of multiple surgeries we obtain a functional and aesthetic result thanks to the adequate utilization of the expanded tissue and of the remnant tissue complemented in one patient with a prosthetic mesh (AU)

Humanos , Feminino , Adolescente , Cloaca/anormalidades , Parede Abdominal/anormalidades , Procedimentos Cirúrgicos Reconstrutivos/métodos , Cloaca/cirurgia , Mesoderma/fisiopatologia , Parede Abdominal/cirurgia , Anormalidades da Pele/cirurgia
Acta otorrinolaringol. esp ; 59(8): 384-389, oct. 2008. ilus
Artigo em Espanhol | IBECS | ID: ibc-67795


Objetivo: Estudiar el desarrollo de la articulación incudoestapedial en embriones y fetos humanos. Material y método: Se han estudiado 46 huesos temporale scon ejemplares comprendidos entre 9 mm y recién nacidos. Las preparaciones estaban cortadas en serie y teñidas con la técnica de tricrómico de Martins. Resultados: La articulación incudoestapedial adquiere las características de una articulación sinovial de tipo enartrosisa las 16 semanas de desarrollo. El cartílago que recubre las superficies articulares está formado por diferentes estratos que se desarrollan sucesivamente: el superficial, a las 19 semanas; el de transición, entre las 20 y las 23 semanas, y el radial, a partir de las 24 semanas. El hueso subcondral se desarrolla a partir de las 29 semanas por los mecanismos de aposición y extensión del periostal y el endostal, pero no es hasta la semana 34 cuando recubre por completo las superficies articulares, constituidos los fascículos óseos por los que se transmitirán las líneas de fuerza. La cápsula articularse forma a partir de la interzona, la zona superficial desarrolla el ligamento capsular y la interna, la sinovial. Conclusiones: En el momento del nacimiento la articulación incudoestapedial está completamente desarrollada (AU)

Objective: To study the development of the incudostapedial joint in human embryos and foetuses. Material and method: 46 temporal bones with specimens between 9 mm and new-borns were studied. The preparations were sliced serially and dyed using the Martins trichrome technique. Results: The incudostapedial joint takes on the characteristics of a spheroidal joint at 16 weeks of development. The cartilage covering the articular surfaces is formed by different strata that develop in succession: the superficial stratum at 19 weeks, the transitional between 20 and 23 weeks, and the radial from 24 weeks on. The subchondral bone develops after 29 weeks by the mechanisms of apposition and extension of the periosteal and endosteal bones, but it is not until week 34 that it completely covers the articular surfaces, following constitution of the bone fascicles transmitting the lines of force. The articular capsule is formed from the inter-zone, the surface zone develops the capsular ligament, and the internal surface develops the synovial membrane. Conclusions: At the time of birth, the incudostapedial joint is completely developed (AU)

Humanos , Reflexo Acústico/genética , Reflexo Acústico/fisiologia , Desenvolvimento Embrionário e Fetal/genética , Desenvolvimento Embrionário e Fetal/fisiologia , Osso Temporal/crescimento & desenvolvimento , Estribo/fisiologia , Cartilagem Articular , Bigorna/fisiologia , Origem da Vida , Mesoderma/fisiologia , Epífises/crescimento & desenvolvimento
Cir. plást. ibero-latinoam ; 34(1): 71-79, ene.-mar. 2008. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-64980


La obtención de tejido adiposo supone un nuevo y prometedor mercado de trabajo para los cirujanos plásticos, ya que los bancos de tejidos escogerán de forma acertada la grasa como el medio más fácil para obtener fuentes de células madre de alto rendimiento, en la medida en que este tejido es capaz de producir al menos cinco veces más unidades formadores de colonias (UFCs) que la médula ósea. El objetivo del presente trabajo es mostrar lo que se puede esperar del tejido adiposo como origen de células adultas de fracción vacularestromal (FVE), y señalar las mejores áreas del cuerpo humano para ser elegidas como donantes de tejido adiposo, extraído mediante liposucción. Describimos la rutina seguida para la obtención de células de FVE mediante la digestión de las muestras de tejido adiposo humano con colagenasa. En el momento de su recolección, esas células presentaban una viabilidad de 92+/- 1% basada en exclusión por Azul de Trypan. Las células de FVE recontadas después de permanecer48 horas en medio de cultivo de Eagle modificado por Dulbecco(DMEM), dentro de una cámara de Neubauer, tras lo cual el rendimiento medio de las células de FVE fue de 7,2 +/- 1,3 x 103células por mililitro de tejido lipoaspirado. En conclusión, pensamos que supone un desafío en la actualida del mejorar las estrategias para la obtención de células de FVE. Este trabajo, por ahora preliminar, muestra que las células de FVE pueden ser fácilmente obtenidas por medio de lipoaspiración. La comparación entre las diferentes áreas donantes, mostró un rendimiento22% más alto para las células de FVE cuando el tejido adiposo había sido obtenido del tronco, en comparación a cuando lo había sido delos miembros (AU)

The harvest of adipose tissue will be a promising labor marketing for plastic surgeons, since tissue banks will certainly choose fat as the easiest way to obtain a high-yield source of stem cells, as this type of tissue can produce at least five times more colony-forming units (CFUs) than bone marrow extracts. The aim of this study is to show what can be expected from fat tissues as an origin of adult stromal vascular fraction (SVF) cells, and to evaluate the best areas to be elected as donor sites within the human body, all obtained by liposuction. The routine to obtain SVF cells by collagenase digestion of human adipose tissue samples was described. At the time of harvest, these cells displayed a viability of 92+/- 1% based on Try pan Blue exclusion, SVF cells were counted after 48 hours culture in Dulbecco´s modified Eagle medium (DMEM) in a Neuberger counting chamber. The average yield of SVF cells was 7,2 +/- 1,3 x 103 cells per millilitre of liposuctioned tissue. As a conclusion, best strategies to obtain SVF cells are an important challenge nowadays. This study, although preliminary, showed that SVF may be easily obtained From liposuction. Comparison among different donor sites showed a 22% higher yield of SVF cells when fat tissue had been obtained from the trunk regions, when confronted with limbs (AU)

Células-Tronco/fisiologia , Topografia Médica/métodos , Tecido Adiposo/citologia , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/ultraestrutura , Células-Tronco/citologia , Lipectomia/tendências , Tecido Adiposo , Pregas Cutâneas , Mesoderma/citologia , Lipectomia/educação , Lipectomia/métodos
Rev. esp. med. nucl. (Ed. impr.) ; 27(2): 112-117, mar.2008. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-66007


Presentamos el caso de un paciente con tumor del estroma gastrointestinal (GIST) en el que se realizaron dos estudios de tomografía por emisión de positrones (PET) con 18F-fluorodeoxiglucosa (18F-FDG), el primero antes de iniciar tratamiento con Glivec y el segundo al mes del inicio del tratamiento. La primera PET 18F-FDG detectó múltiples lesiones en el esófago distal, el hígado y en una adenopatía interaortocava. La segunda PET 18F-FDG mostró muy buena respuesta al tratamiento, con una remisión prácticamente completa de la enfermedad. Tras un seguimiento de 23 meses se confirma la respuesta metabólica detectada precozmente con PET 18F-FDG. Se discute la utilidad de la PET 18F-FDG en la valoración de la respuesta al tratamiento en el GIST y se compara con la tomografía axial computarizada (TAC)

We present the case of a male patient with gastrointestinal stromal tumor (GIST) in whom we conducted two 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET) studies, the first one prior to beginning the treatment with Glivec® and the second after one month of treatment. The first 18F-FDG PET scan detected multiple FDG avid foci in distal esophagus, liver and in an interaortocava lymph node. The second 18F-FDG PET showed very good response to therapy, with an almost complete disease remission. After 23 months of follow-up, the early response to treatment detected by 18F-FDG PET was confirmed. The utility of 18F-FDG PET in the evaluation of response to treatment in GIST is discussed and compared with CT

Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Gastrointestinais/tratamento farmacológico , Células Estromais/patologia , Tomografia Computadorizada de Emissão/métodos , Fluordesoxiglucose F18 , Mesoderma , Proteínas Tirosina Quinases/antagonistas & inibidores
Gastroenterol. hepatol. (Ed. impr.) ; 30(7): 391-394, ago. 2007. ilus
Artigo em Espanhol | IBECS | ID: ibc-62484


El sarcoma indiferenciado (embrionario) del hígado es una neoplasia primaria maligna, que se diagnostica de forma excepcional en el adulto. La ausencia de síntomas específicos, el rápido crecimiento tumoral y la normalidad de los marcadores tumorales hacen de esta neoplasia un proceso de mal pronóstico. Histológicamente, se caracteriza por la presencia de una matriz mesenquimal y un pleomorfismo nuclear. Diversas características histológicas y cromosómicas harían pensar en una posible relación con el hamartoma mesenquimal. El tratamiento de elección es la cirugía, y puede recurrirse a la quimioterapia como tratamiento coadyuvante

Undifferentiated (embryonal) sarcoma (UES) of the liver is a primary malignant tumor, rarely diagnosed in adults. Because of the absence of specific symptoms, rapid tumor growth, and normality of the common tumor markers, this neoplasm has a poor prognosis. Histologically, UES of the liver is characterized by anaplastic cells within myxoid matrix. Histological, immunohistochemical and chromosomic alterations are similar in UES and in mesenchymal hamartoma, suggesting a relation between these entities. The mainstay of treatment is surgery, while adjuvant treatment could increase survival

Humanos , Masculino , Pessoa de Meia-Idade , Anemia Hipocrômica/diagnóstico , Anemia Hemolítica/diagnóstico , Neoplasias Hepáticas/complicações , Anemia Hipocrômica/etiologia , Anemia Hemolítica/etiologia , Lipossarcoma/complicações , Biomarcadores Tumorais/análise , Mesoderma/patologia , Hamartoma/patologia
Clin. transl. oncol. (Print) ; 9(7): 420-428, jul. 2007. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-123333


The Hedgehog (Hh) family of intercellular signalling proteins have come to be recognised as key mediators in many fundamental processes in embryonic development. Their activities are central to the growth, patterning and morphogenesis of many different regions within the bodies of vertebrates. In some contexts, Hh signals act as morphogens in the dose-dependent induction of distinct cell fates within a target field, in others as mitogens in the regulation of cell proliferation or as inducing factors controlling the form of a developing organ. These diverse functions of Hh proteins raise many intriguing questions about their mode of action. Various studies have now demonstrated the function of Hh signalling in the control of cell proliferation, especially for stem cells and stem-like progenitors. Abnormal activation of the Hh pathway has been demonstrated in a variety of human tumours. Hh pathway activity in these tumours is required for cancer cell proliferation and tumour growth. Recent studies have uncovered the role for Hh signalling in advanced prostate cancer and demonstrated that autocrine signalling by tumour cells is required for proliferation, viability and invasive behaviour. Thus, Hh signalling represents a novel pathway in prostate cancer that offers opportunities for prognostic biomarker development, drug targeting and therapeutic response monitoring (AU)

Humanos , Animais , Masculino , Proteínas Hedgehog , Proteínas Hedgehog/metabolismo , Neoplasias da Próstata/diagnóstico , Neoplasias da Próstata/metabolismo , Transdução de Sinais/genética , Proteínas Hedgehog/genética , Células Epiteliais/metabolismo , Mesoderma/metabolismo , Modelos Biológicos , Próstata/metabolismo , Próstata/patologia
Acta otorrinolaringol. esp ; 58(1): 4-6, ene. 2007. ilus
Artigo em Espanhol | IBECS | ID: ibc-053714


Objetivo: Determinar si existen conexiones entre la médula ósea de los osículos timpánicos y el mesénquima que rellena la futura cavidad timpánica. Material y métodos: Se han examinado 90 huesos temporales pertenecientes a embriones y fetos, y se han seleccionado 15 de edades comprendidas entre las semanas 20 y 30 del desarrollo al presentar conexiones entre médula del osículo y el mesénquima. Resultados: Las conexiones son de tipo transitorio y aparecen en el martillo y el yunque entre las semanas 20 y 24 de desarrollo, mientras que en el estribo se manifiestan posteriormente, entre las semanas 24 y 28. Conclusiones: Estas conexiones pueden tener un papel importante en la fagocitosis de los restos mesenquimales y sumarse a los mecanismos de eliminación de los detritus producidos durante la regresión

Objective: To investigate the presence of connections between the bone marrow of the ossicles and the mesenchyme that fills the future tympanic cavity. Material and methods: Ninety temporal bones from embryos and foetuses were examined, selecting 15 aged between 20th to 30th weeks of development, to show connections between ossicle marrow and mesenchyme. Results: The connections are transitory and appear in the malleus and the incus between 20th to 24th weeks of development, while in the stapes appear later, being between 24th to 28th weeks. Conclusions: These connections may have an important role in the phagocytosis of the mesenchymal remains and join in the detritus elimination mechanisms produced during the regression

Humanos , Ossículos da Orelha/embriologia , Medula Óssea/embriologia , Mesoderma , Osso Temporal/embriologia , Fagocitose , Idade Gestacional