Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 4.243
Filtrar
1.
Enferm. clín. (Ed. impr.) ; 29(5): 313-317, sept.-oct. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-184157

RESUMO

Objetivo: Conocer la opinión de un grupo de pacientes jóvenes ante el personal sanitario con tatuajes. Método: Estudio descriptivo realizado en el primer semestre de 2017 en las pacientes ingresadas en la Unidad de Obstetricia del Complexo Hospitalario de Ourense. La recogida de datos se realizó mediante un cuestionario autoadministrado de elaboración propia, donde se mostraban varias fotografías de profesionales sanitarios con y sin tatuajes. Se realizó un análisis descriptivo de las variables y se realizaron pruebas no paramétricas para determinar la asociación potencial entre las variables, utilizándose el paquete estadístico SPSS 15.0(R). Resultados: Participaron un total de 66 pacientes. Independientemente de si las participantes del estudio tuviesen o no tatuajes, todas puntuaron mejor a los profesionales sanitarios no tatuados, en comparación con los tatuados, aunque estas diferencias son menores en las participantes que a su vez tienen tatuajes. No se han encontrado datos significativos en ninguno de los casos. Conclusiones: Los participantes de nuestro estudio valoran peor a los profesionales sanitarios tatuados en relación con su profesionalidad, credibilidad y cercanía e implicación en comparación con sus homólogos sin tatuajes


Objective: To learn the opinion of a group of young patients about health personnel with tattoos. Method: Descriptive study conducted in the first semester of 2017 on patients admitted to the Obstetrics Unit of the Hospital Complex of Ourense. The data collection was carried out by means of a self-administered questionnaire prepared "in-house" where several photographs of health professionals with and without tattoos were shown. A descriptive analysis of the variables and nonparametric tests were carried out to determine the potential association between the variables, using the statistical package SPSS 15.0(R). Results: A total of 66 patients participated. Regardless of whether or not the study participants had tattoos themselves, they all scored the non-tattooed better than the tattooed health professionals, although these differences were less in the participants who had tattoos. No significant data were found in any of the cases. Conclusions: The participants of our study scored tattooed health professionals more poorly in terms of their professionalism, credibility, closeness and involvement compared to their counterparts without tattoos


Assuntos
Animais , Adolescente , Adulto Jovem , Adulto , Opinião Pública , Pessoal de Saúde , Tatuagem , Estigma Social , Aparência Física , Relações Profissional-Paciente , Inquéritos e Questionários , Profissionalismo
2.
Nutr. hosp ; 36(5): 1163-1170, sept.-oct. 2019. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-184641

RESUMO

Introduction: aberrant crypt foci (ACF) are colon preneoplastic lesions that can be used as a tool to study preventive processes for colorectal cancer (CRC). This model consists of initiation induced by azoxymethane (AOM) and promoted by sodium dextran sulfate (DSS), simulating human colonic carcinogenesis in a rat model. There is no direct information on the effects of this process on nutritional markers. Objective: to determine the effect on nutritional markers after the induction of ACF by AOM/DSS in a rat model. Methods: ACF were induced in 24 four-week-old Sprague Dawley male rats by administration of 2 AOM injections (10 mg/kg) and 7 days of 2% DSS in their drinking water. Body weight gain, food and fluid intake, weight of sacrificial organs, nutritional biochemical profiles, liver and kidney toxicity were evaluated. Cell counts in blood were also performed and histological sections evaluated in specific organs. The model was confirmed with identification and counts of ACF. Half of the rats were sacrificed at the sub-chronic stage and the rest at the chronic stage. Results: at the sub-chronic stage, changes in the liver and colon weight, and in the lymphocyte count were observed. For both stages, histopathological damage was observed in liver, kidney and colon, along with alterations in serum glucose levels. Conclusions: the model for proposed ACF can be used at the sub-chronic stage without the need for observation at the chronic stage. More research is needed to determine the mechanism of the observed effects


Introducción: los focos de criptas aberrantes (ACF) son lesiones preneoplásicas en colon que pueden ser utilizados como herramienta para estudiar procesos preventivos para el cáncer colorectal (CCR). Este modelo consiste en la iniciación inducida por azoximetano (AOM) y promovida por dextrano sulfato sódico (DSS) simulando una carcinogénesis colónica humana en un modelo de rata. No existe información directa de los efectos sobre marcadores nutricios para este proceso. Objetivo: determinar el efecto sobre marcadores nutricios tras la inducción de ACF por AOM/DSS en un modelo de rata. Métodos: se utilizaron veinticuatro ratas machos Sprague Dawley de 4 semanas para la inducción de ACF por administración de 2 inyecciones de AOM (10 mg/kg) y 7 días de DSS al 2% en el agua para beber. Se evaluó la ganancia de peso corporal, el consumo de alimento y de líquidos, el peso de órganos al sacrificio, perfiles bioquímicos nutricios, de toxicidad hepática y renal. Asimismo, se realizaron conteos celulares en sangre y se evaluaron cortes histológicos en órganos específicos. El modelo se confirmó con la identificación y conteos de ACF. Se sacrificó la mitad de las ratas en etapa subcrónica y las demás en etapa crónica. Resultados: en la etapa subcrónica se observaron cambios entre grupos en el peso del hígado y colon, y en el conteo de linfocitos. En ambas etapas se observaron daños histopatológicos en hígado, riñón y colon, así como alteraciones en los niveles de glucosa sérica. Conclusiones: el modelo para ACF propuesto puede ser utilizado en etapa subcrónica sin necesidad de llevarlo a tiempo crónico. Es necesaria más investigación para determinar el mecanismo de los efectos observados


Assuntos
Animais , Masculino , Ratos , Focos de Criptas Aberrantes/induzido quimicamente , Lesões Pré-Cancerosas/induzido quimicamente , Lesões Pré-Cancerosas/veterinária , Lesões Pré-Cancerosas/diagnóstico , Estudos Longitudinais , Modelos Animais de Doenças , Ratos Sprague-Dawley
3.
Endocrinol. diabetes nutr. (Ed. impr.) ; 66(7): 434-442, ago.-sept. 2019. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-182863

RESUMO

Background: Non-alcoholic fatty liver disease (NAFLD), a condition that leads to fibrosis, is caused by intake of very high-fat diets (HFDs). However, while the negative impact on the liver of these diets has been an issue of interest, systematic research on the effect of HFDs are lacking. Objective: To characterize the overall impact of HFDs on both molecular and morphological signs of liver remodeling. Methods: A study was conducted on male C57BL/6J mice to assess the effect of 4- and 8-week HFDs (60% kcal from fat) on (I) liver steatosis and fibrosis, and (II) expression of factors involved in inflammation and angiogenesis. Results: After an 8-week HFD, vascular endothelial growth factor type-2 receptor (VEGF-R2) and fatty acid translocase/trombospondin-1 receptor (CD36) were overexpressed in liver tissue of mice given HFDs. These changes suggest impaired liver angiogenesis and occurred together with (I) increased GPR78-BiP and EIF2alpha phosphorylation, suggesting endoplasmic reticulum stress, (II) induction of Col1a1 gene expression, a marker of fibrosis, and (III) increased CD31 immunolabeling, consistent with active angiogenesis and fibrosis. Conclusion: Our data show that very HFDs promote a rapid inflammatory response, as well as deregulation of angiogenesis, both consistent with development of liver fibrosis


Antecedentes: El hígado graso no alcohólico (HGNA) es una enfermedad hepática que ocasiona fibrosis y se genera por la ingesta de dietas ricas en grasa. Aunque los efectos nocivos de este tipo de dietas son de gran interés, no son muy abundantes los estudios sistemáticos sobre las consecuencias que su consumo puede tener en el hígado. Objetivo: Evaluar los efectos de una dieta rica en grasa sobre el remodelado hepático, tanto a nivel morfológico como molecular. Métodos: Se utilizaron ratones macho C57BL/6J tratados durante 4/8 semanas con una dieta que contenía un 60% de las kilocalorías procedentes de grasa sobre: 1) la aparición de esteatosis y/o fibrosis hepática y 2) la expresión de factores implicados en procesos de inflamación y angiogénesis. Resultados: Tras 8 semanas de dieta se observó un incremento en el receptor del factor de crecimiento vascular endotelial tipo 2 (R2-FCVE) y en la translocasa de ácidos grasos (CD36). Estos cambios, que sugieren que los procesos angiogénicos del hígado están alterados, fueron simultáneos a: 1) un aumento del GPR78-BiP y de la fosforilación de EIF2alpha, marcadores de estrés del retículo endoplásmico, 2) la inducción en la expresión del gen de colágeno Col1a1, indicador de fibrosis y 3) un incremento de células inmunofluorescentes para CD31 compatible con procesos de angiogénesis y de fibrosis. Conclusiones: Nuestros resultados muestran que las dietas con alto contenido en grasa inducen la rápida activación de respuestas inflamatorias, así como alteraciones en la angiogénesis, siendo ambos procesos compatibles con el desarrollo de fibrosis hepática


Assuntos
Animais , Camundongos , Gorduras na Dieta/efeitos adversos , Dieta Hiperlipídica/efeitos adversos , Inibidores da Angiogênese , Fígado Gorduroso/complicações , Fígado Gorduroso/veterinária , Inflamação/complicações , Fatores de Crescimento Endotelial/administração & dosagem , Antígenos CD36 , Fibrose , Projetos de Pesquisa , Western Blotting
4.
Med. intensiva (Madr., Ed. impr.) ; 43(6): 362-372, ago.-sept. 2019. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-183255

RESUMO

El traumatismo craneoencefálico (TCE) es una de las patologías más importantes en la actualidad, ya que afecta a un alto porcentaje de individuos de todas las edades. A pesar de los avances en el campo del diagnóstico, la monitorización y el tratamiento del TCE, quedan importantes cuestiones sin resolver alrededor de la fisiopatología de este tipo de traumatismo. Con el fin de profundizar en dicho conocimiento y poder evaluar y aplicar un posible tratamiento que resulte eficaz para estos pacientes, se han desarrollado diferentes modelos experimentales que simulan los mecanismos de acción y el cuadro clínico del TCE. A su vez, cada modelo representa un determinado tipo de traumatismo y evalúa un aspecto concreto de la cascada fisiopatológica desencadenada tras el TCE. El objetivo de este trabajo es detallar los principales modelos experimentales que abordan la lesión cerebral tras un TCE, así como su potencial traslación a la práctica clínica diaria


Traumatic brain Injury (TBI) is a major public healthcare concern, affecting people of all ages. Despite advances in the diagnosis, monitoring and clinical management of TBI, many unresolved questions remain regarding its physiopathology. In an attempt to understand the pathological features of TBI and to evaluate single potential therapeutic strategies, various animal models have been developed to simulate the mechanisms of action and the clinical manifestations of TBI patients. In turn, each model represents a specific type of trauma and evaluates a specific physiopathological aspect of the cascade triggered as a result of TBI. This review describes the main experimental models currently available referred to TBI and their possible application to the clinical setting


Assuntos
Animais , Modelos Animais , Traumatismos Cranianos Penetrantes/veterinária , Técnicas In Vitro/instrumentação , Lesões Encefálicas Traumáticas/fisiopatologia , Técnicas In Vitro/métodos , Lesões Encefálicas/veterinária
6.
Eur. j. anat ; 23(5): 325-332, sept. 2019. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-183862

RESUMO

Liver ischemia reperfusion is induced during surgical procedures like liver transplantation and resection. Multiple mechanisms have been postulated to liver damage following liver ischemia reperfusion injury, such as oxidative stress and inflammatory reactions. The present study declares the possible mechanism of tadalafil, toward modulating the inflammatory response. Forty-eight rats were divided into 4 groups as follows; Sham group subjected to midline laparotomy only. Tadalafil group administered Tadalafil 10 mg/kg intraperitoneal 45 min before sham operation. I/R (Ischemiareperfusion) group, rats undergo 60 min of hepatic ischemia followed by 60 min of reperfusion. Tadalafil + I/R group rats undergo a similar pattern of I/ R after the treatment with Tadalafil 10 mg/kg, 45 min before ischemia. At the end of the reperfusion, the blood samples were collected for estimation of biochemical markers including liver enzymes using colorimetric assay method and serum: TNF-α (tumor necrosis factor-α), IL-6 (interleukin 6) levels, ICAM- 1 (Intercellular Adhesion Molecule-1) were measured. Tissues were evaluated by semiquantitative and morphometrical approaches. Tadalafil succeeded in restoring normal levels of liver enzymes and ameliorating the oxidative stress as evidenced by decreasing MDA and restoring reduced glutathione levels in liver tissue homogenate. Also, Tadalafil exhibits anti-inflammatory effects, as it significantly decreased the levels of TNF-α, IL6 and ICAM-1. The findings are supported by BCL-2, TNF-α immunomarkers. It is concluded that modulation of the inflammatory response might be one of the mechanisms of Tadalafil-mediated hepatoprotection, so it is recommended as an adjuvant therapy in liver surgery


No disponible


Assuntos
Animais , Ratos , Traumatismo por Reperfusão/veterinária , Estresse Oxidativo , Transplante de Fígado/veterinária , Apoptose/efeitos dos fármacos , Fator de Necrose Tumoral alfa , Tadalafila/administração & dosagem , Fígado/anatomia & histologia , Fígado/enzimologia , Injeções Intraperitoneais/veterinária , Imuno-Histoquímica
7.
Eur. j. anat ; 23(5): 361-368, sept. 2019. ilus
Artigo em Inglês | IBECS | ID: ibc-183866

RESUMO

Peg10 (paternally expressed 10) is a retrotransposon-derived gene that is highly conserved across mammalian species. Peg10 is involved in cell proliferation and differentiation, and is essential for placenta formation in mice. Although a number of studies have examined Peg10 expression in the placenta, its cellular localization in the brain is still unclear. The function of Peg10 in the brain is also unknown. Here, we examined Peg10 distribution in the mouse brain. In situ hybridization revealed intense expression of the gene in the core region of the accumbens nucleus, lateral division of the bed nucleus of the stria terminalis, medial preoptic nucleus, paraventricular nucleus, arcuate nucleus, dorsomedial hypothalamic nucleus, premammillary nucleus, central amygdaloid nucleus and lateral parabrachial nucleus. Moderate to intense expression of Peg10 was also observed in monoaminergic nuclei such as the substantia nigra, dorsal raphe nucleus and locus coeruleus. These results suggest that Peg10 may play a role in motivational processes, emotional regulation, and autonomic functions in the brain. The findings also suggest that Peg10 may have contributed to the evolution of mammals, not only by participating in placenta formation, but also by regulating parental behavior and hormonal secretions necessary for maternal responsiveness


No disponible


Assuntos
Animais , Camundongos , Cérebro/anatomia & histologia , Hipotálamo/anatomia & histologia , Sistema Límbico/anatomia & histologia , Proteínas/genética , Hibridização in Situ Fluorescente/veterinária , Hipocampo/anatomia & histologia , Diencéfalo/anatomia & histologia
8.
Cient. dent. (Ed. impr.) ; 16(2): 155-160, mayo-ago. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-183728

RESUMO

Las extracciones dentarias producen una pérdida ósea en sentido horizontal y vertical, que conllevan alteraciones funcionales para los pacientes, y dificultan la colocación de implantes dentales para los profesionales. Para minimizar esta pérdida ósea, se utilizan diferentes materiales de injerto, entre los cuales destaca el injerto autógeno, por cumplir las características de osteogénesis, osteoconducción y osteoinducción. En el año 2010 se describe por primera vez la utilización de dentina como material de injerto autógeno, demostrando que este material puede ser una alternativa terapéutica al resto de materiales de injerto, al permitir la osteoconducción y la osteoinducción, y mostrar la formación de hueso nuevo en un 46-87% del área con injerto de dentina autógena, 3 meses después de su utilización. La última revisión sistemática publicada en el año 2018, concluyó que los implantes colocados en áreas regeneradas en zonas en las que se empleó dentina como material de injerto, presentaron tasas de supervivencia del 97,7% después de 1 año de seguimiento, sugiriendo este nuevo material como una alternativa con resultados prometedores, aunque son necesarios más estudios al respecto


Subsequent to tooth extraction, a reduction of the length and width of alveolar ridge can be observed. It causes functional alterations to patiens, and problems to proper insertion of dental implants. In order to prevent this bone atrophy, different graft materials can be used, being considered autogenous graft the best because allows osteogenesis, osteoconduction and osteoinduction. In 2010 it was first published the use of autogenous dentine as a graft material, showing it could be an ideal graft material, as a material with excellent osteoconduction and osteoinduction. Besides, this graft material is slowly absorbed and replaced by new bone, in 46-87% of the áreas grafted with dentine, 3 months after regeneration. Last systematic review published in 2018 concluded dental implants inserted in regenerated areas with autogenous dentine had survival rates of 97,7% for over a year follow-up, so this new material is considered an alternative with good results, but there are necessary more studies with long term follow-up


Assuntos
Humanos , Animais , Cirurgia Bucal/instrumentação , Transplante Autólogo/métodos , Osteogênese , Regeneração Óssea , Dentina/metabolismo , Dentina Secundária/crescimento & desenvolvimento , Dentinogênese/fisiologia , Odontoblastos/fisiologia
9.
Eur. j. anat ; 23(4): 243-251, jul. 2019. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-182997

RESUMO

Atomoxetine (ATX) is a noradrenaline reuptake inhibitor used to treat Attention deficit hyperactive syndrome (ADHD), or improve cognition in normal subjects. The cognitive effects of ATX require inputs from the hippocampus. Moreover, proliferation is said to be located in the dentate gyrus (DG) of the hippocampus.In the present study, we hypothesised that ATX improves memory and proliferation of the adult rat hippocampus. To test this hypothesis, 5 intraperitoneal injections of ATX (30 mg/kg/day) over 5 consecutive days were delivered to rats. 30 minutes after the last injection, spatial memory was tested using the Novel location recognition (NLR) test. Proliferation of hippocampal cells was quantified using immunohistochemistry for the proliferative marker Ki67. ATX-treated rats showed cognitive enhancement in the NLR task and increase in cell proliferation in the Subgranular zone (SGZ) of the DG, compared to saline-treated controls. The results demonstrate that ATX is able to enhance cognition through increasing the levels of proliferation in the adult rat brains


No disponible


Assuntos
Animais , Adulto , Ratos , Cloridrato de Atomoxetina/farmacologia , Cérebro/efeitos dos fármacos , Transtorno do Deficit de Atenção com Hiperatividade/tratamento farmacológico , Estimulantes do Sistema Nervoso Central/farmacologia , Hipocampo/anatomia & histologia , Transtorno do Deficit de Atenção com Hiperatividade/metabolismo , Imuno-Histoquímica , Habituação Psicofisiológica/efeitos dos fármacos , Modelos Animais de Doenças , Neurogênese/efeitos dos fármacos
10.
Eur. j. anat ; 23(4): 267-272, jul. 2019. ilus
Artigo em Inglês | IBECS | ID: ibc-183000

RESUMO

Ayurvedic medicines is known to use heavy metals in their preparation. Nagabhasma is one such form of a lead-based medicine. Even though lead is known to be toxic to several systems of the human body, according to Ayurveda, the metallic toxicity of the lead gets nullified and thereby imbibes medicinal property when it is prepared using many herbs and stringent traditional methods. Therefore, the present study is designed to evaluate the effect of such detoxified lead in various stages of authentically prepared Nagabhasma on the histopathology of liver in comparison with lead acetate and commercially available Nagabhasma-administered animals. Less than the human-equivalent doses of Nagabhasma at four intermittent stages of its preparation were administered orally for 30 days and 60 days (short term and long term exposure) to Wistar rats. In another set of experiment, test-material-administered animals were kept under observation for an additional period of two months to record the residual effect. Immediately after the administration and after the observation period, the animals were sacrificed to collect the liver for histopathological examination. The histopathological results of the immediate and residual effects showed varying alterations in the microarchitecture of the liver as the stages of Nagabhasma preparation advanced. The final product (stage 4 bhasma), showed very less toxic effect in comparison with other stages. In conclusion, the results state that, by following the traditional procedures while preparing Nagabhasma, the metallic lead gets converted into non-toxic organometallic compound


No disponible


Assuntos
Humanos , Animais , Metais Pesados/toxicidade , Medicina Ayurvédica/efeitos adversos , Fígado/efeitos dos fármacos , Chumbo/toxicidade , Hepatócitos/efeitos dos fármacos , Capilares/efeitos dos fármacos , Fígado/anatomia & histologia , Fígado/patologia , Ratos Wistar , Vasos Sanguíneos/anatomia & histologia , Vasos Sanguíneos/patologia
11.
Eur. j. anat ; 23(4): 289-300, jul. 2019. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-183003

RESUMO

Many artemisinin-based combination therapies (ACTs) have been approved for malaria treatment, yet reports indicate that some ACTs pose reversible testicular toxicity; however there is no comparative study of these ACTs on the testes in a curative malarial model. We investigated the ameliorative activity of six ACTs on Plasmodium berghei (PB) induced perturbations in testicular antioxidants, serum testosterone levels, sperm motility and the testes microanatomy. Forty male Swiss mice were divided into 8 groups of 5 each: Group 1 normal control (NC), uninfected and untreated, received placebo; group 2 was parasitized non-treated (PNT), while groups 3 - 8 received PB inoculum intraperitoneally. Initial parasitemia was established after 72 hours. Groups 3 - 8 thereafter received oral therapeutic doses of artesunate/amodiaquine (PBAA), artesunate/mefloquine (PBAM), artesunate/sulfadoxine-pyrimethamine (PBASP), artemisinin-piperaquine (PBAP), dihydroartemisinin/piperaquine (PBDP) and artemether/lumefantrine (PBAL) per kg body weight respectively. final parasitemia was performed 24 hours after last treatment, and animals euthanized. Result for parasitemia level was significantly (p < 0.05) declined in ACT-treated groups, except PBASP compared with PNT. Enzymatic antioxidants were significantly (p < 0.0001) altered in ACT-treated groups compared to PNT. Non-enzymatic antioxidants were significantly (p < 0.0001) increased in PBDP compared to NC and PNT. Progressive sperm motility significantly (p < 0.0001) declined in PNT, PBASP, PBAP and PBDP groups compared to NC. Testosterone showed decreasing trend in PBAP compared to PNT, and severe testicular distortions were demonstrated in PNT, PBASP, PBAP and PBDP. This study concludes that therapeutic doses of AA, AM and AL moderately protects against the deleterious effects of Plasmodium berghei-induced testicular toxicity in Swiss mice


No disponible


Assuntos
Animais , Camundongos , Artemisininas/administração & dosagem , Plasmodium berghei/efeitos dos fármacos , Testículo/anatomia & histologia , Testículo/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Antioxidantes/toxicidade , Artesunato/administração & dosagem , Projetos de Pesquisa , Testosterona/análise , Ensaio de Imunoadsorção Enzimática , Testículo/patologia
12.
Eur. j. anat ; 23(4): 307-312, jul. 2019. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-183005

RESUMO

Monkey and other hominids species have 5 sacral segments in 10% cases. The similar frequencies for each 6-segmented and 4-segmented sacra in human are known as lumbo-sacral transitional vertebra (LSTV). Achieving the erect posture in human has necessitated much skeletal modification, but these are more apparent in the lumbosacral region. Sacral kyphosis is a distinguishing feature of the human sacrum, which helps to differentiate them from the animal. The monkey has a sacral index near 80, and humans a sacral index is near 100. The sacral index was 88 in six-segmented sacra with negligible sacral kyphosis, having sacralisation of the 5th lumbar vertebra. Therefore, SI is 88 and lack of sacral kyphosis challenge its human origin. On the contrary, gross morphology, actual sacral index, and comparison with apes gave sufficient evidence of human origin. Later excluding 5th Lumbar vertebra, the sacral index is 107.34 and might belong to a male which corresponds with bone bank record


No disponible


Assuntos
Humanos , Animais , Região Lombossacral/anatomia & histologia , Cifose/complicações , Sacro/anormalidades , Sacro/anatomia & histologia , Região Lombossacral/anormalidades , Antropometria , Sacro/diagnóstico por imagem , Primatas/anormalidades
13.
Int. microbiol ; 22(2): 227-237, jun. 2019. graf, tab
Artigo em Inglês | IBECS | ID: ibc-184829

RESUMO

Chicken feather waste is generally insufficiently utilized despite its high content of protein, constituting an environmental issue. Biodegradation of the waste with enabling microbes provides an advantageous option among the available solutions. In this study, an efficient whole feather-degrading strain was strategically isolated from a soil sample taken from a local tea plantation that has little or nothing to do with feathers. The strain was identified as Bacillus thuringiensis (designated as FDB-10) according to the cloned complete 16S rRNA sequence. The FDB-10 could efficiently degrade briefly heat-treated whole feather (102 °C, 5 min; up to 90% of a maximum concentration of 30 g/L) in a salt medium supplemented with 0.1 g/L yeast extract within 24 h (37°C, 150 rpm). Addition of carbon sources (glycerol, glucose, starch, Tween 20, Tween 80, 1.25 g/L as glycerol) to the fermentation medium could improve the degradation. However, significant inhibition could be observed when the added carbon source reached the amount usually adopted in the investigation of carbon source preference (1%). Nitrogen source (NH4Cl, (NH4)2SO4, peptone) adversely influenced the performance of the strain. When the molar concentrations of NH4+ were equal for the two salt, the inhibitory effect on degradation of whole feathers was similar. Entirely different from other reported feather-degrading strains showing a preference to melanin-free feather substrates, the strain isolated in this study could degrade melanin-containing feather equally efficiently, and higher protease activity could be detected in the digest mix. As a plus, the strain could degrade feathers in rice wash produced in daily cooking, indicating its potential use in the simultaneous treatment of rice cooker wastewater produced by a rice processing plant. All these results imply that the FDB-10 is a strain with great potential in the biodegradation of feather waste


No disponible


Assuntos
Animais , Bacillus thuringiensis/isolamento & purificação , Plumas/metabolismo , Microbiologia do Solo , Bacillus thuringiensis/genética , Biotransformação , Bacillus thuringiensis/metabolismo , Bacillus thuringiensis/classificação , Galinhas , Análise por Conglomerados , Meios de Cultura , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico , Resíduos Industriais , Queratinas/metabolismo , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Chá/crescimento & desenvolvimento , Temperatura Ambiente
14.
Clín. investig. arterioscler. (Ed. impr.) ; 31(3): 111-118, mayo-jun. 2019. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-182704

RESUMO

The very low-density lipoprotein receptor (VLDLR) plays an important function in the control of serum triglycerides and in the development of non-alcoholic fatty liver disease (NAFLD). In this study, we investigated the role of peroxisome proliferator-activated receptor (PPAR)ß/δ activation in hepatic VLDLR regulation. Treatment of mice fed a high-fat diet with the PPARß/δ agonist GW501516 increased the hepatic expression of Vldlr. Similarly, exposure of human Huh-7 hepatocytes to GW501516 increased the expression of VLDLR and triglyceride accumulation, the latter being prevented by VLDLR knockdown. Finally, treatment with another PPARß/δ agonist increased VLDLR levels in the liver of wild-type mice, but not PPARß/δ-deficient mice, confirming the regulation of hepatic VLDLR by this nuclear receptor. Our results suggest that upregulation of hepatic VLDLR by PPARß/δ agonists might contribute to the hypolipidemic effect of these drugs by increasing lipoprotein delivery to the liver. Overall, these findings provide new effects by which PPARß/δ regulate VLDLR levels and may influence serum triglyceride levels and NAFLD development


El receptor de las lipoproteínas de muy baja densidad (VLDLR) desempeña una función muy importante en el control de los niveles de triglicéridos séricos y en el desarrollo de la enfermedad del hígado graso no alcohólico (EHGNA). En este estudio hemos investigado el papel de la activación del receptor activado por los proliferadores peroxisómicos (PPAR)ß/δ en la regulación hepática del VLDLR. El tratamiento de ratones alimentados con una dieta rica en grasas con el agonista PPARß/δ GW501516 aumentó la expresión hepática de Vldlr. Asimismo, la exposición de hepatocitos humanos Huh-7 a GW501516 aumentó la expresión de VLDLR y la acumulación de triglicéridos, siendo este ultimo aumento evitado por el knockdown de VLDLR. Finalmente, el tratamiento con otro agonista PPARß/δ incrementó los niveles de VLDLR en el hígado de ratones wild-type, pero no en el de ratones deficientes en PPARß/δ, confirmando la regulación del VLDLR hepático por este receptor. En conjunto, nuestros resultados proporcionan un nuevo efecto por el que PPARß/δ regula los niveles de VLDLR y puede influenciar los niveles de triglicéridos séricos así como el desarrollo de la EHGNA


Assuntos
Animais , Camundongos , PPAR beta/efeitos dos fármacos , PPAR beta/metabolismo , Hepatócitos/metabolismo , Lipoproteínas VLDL/agonistas , Lipoproteínas VLDL/metabolismo , Gorduras na Dieta , Triglicerídeos/metabolismo , Western Blotting , Análise de Variância , PPAR beta/farmacologia
15.
Neurología (Barc., Ed. impr.) ; 34(5): 300-308, jun. 2019. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-180846

RESUMO

Introducción: La acetona cianohidrina (ACH) es una sustancia tóxica resultante de la hidrólisis enzimática de linamarina, contenido en las raíces de yuca (Manihot esculenta Crantz); su consumo a largo plazo se asocia con 2 trastornos neurológicos: konzo y la neuropatía atáxica tropical. Estudios anteriores han evaluado las alteraciones conductuales después del consumo de esta sustancia, pero los efectos tóxicos sobre los procesos fisiológicos se desconocen. Método: Se asignaron 32 ratas Wistar macho a 4 grupos experimentales (n = 8): un grupo vehículo (solución salina 0,3 ml/rata, ip) y 3 grupos con ACH (PubChem CID: 6406) a concentraciones de 10, 15 y 20 mM, durante 28 días, cada 24 h. Se evaluó la actividad motora espontánea en campo abierto y la coordinación motora en pruebas de rotarod y nado a 0, 7, 14, 21 y 28 días de tratamiento. Al final de las pruebas conductuales (día 28) se tomaron muestras de sangre por punción transcardiaca para evaluar la función renal y hepática. Resultados: La ACH promovió alteraciones en la actividad locomotora y promovió tanto el nado lateral como la conducta de giro en la prueba de nado los días 21 y 28 del tratamiento. La ACH incrementó los parámetros de la función renal y hepática de una manera dependiente de la concentración, excepto la glucosa y la bilirrubina total. Conclusión: Estos datos indican que el contenido de este compuesto tóxico contenido en las raíces de yuca podría ser potencialmente peligroso bajo el consumo a largo plazo en sujetos vulnerables


Introduction: Acetone cyanohydrin (ACH) is a toxic substance present in cassava roots (Manihot esculenta Crantz) which results from enzymatic hydrolysis of linamarin. Long-term consumption is associated with 2 neurological disorders: konzo and tropical ataxic neuropathy. Previous studies have evaluated behavioural alterations linked to ACH consumption, but the toxic effects of this substance on physiological processes remain unknown. Method: 32 male Wistar rats were assigned to 4 experimental groups (n = 8 per group): a vehicle group (0.3 mL saline solution, IP) and 3 ACH groups (PubChem CID: 6406) dosed at 10, 15, and 20 mM/24h for 28 days. We evaluated spontaneous motor activity with the open field test and motor coordination with the rotarod and forced swimming tests at 0, 7, 14, 21, and 28 days of treatment. At the end of the assessment period (day 28), blood samples were collected by transcardiac puncture to evaluate kidney and liver function. Results: ACH caused alterations in locomotor activity and promoted both lateral swimming and spinning in the forced swimming test at 21 and 28 days of treatment. Furthermore, it led to an increase in the levels of the parameters of kidney and liver function in a concentration-dependent manner, except for glucose and total bilirubin. Conclusion: Our results suggest that long-term consumption of this toxic compound present in cassava roots may be potentially dangerous for vulnerable subjects


Assuntos
Animais , Ratos , Atividade Motora/efeitos dos fármacos , Fígado/efeitos dos fármacos , Rim/efeitos dos fármacos , Neurotoxinas/isolamento & purificação , Acetona/efeitos adversos , Manihot/efeitos adversos , Ratos Wistar , Extratos Vegetais/farmacocinética , Modelos Animais de Doenças , Testes de Função Renal/estatística & dados numéricos , Testes de Função Hepática/estatística & dados numéricos , Testes de Toxicidade Aguda/métodos
16.
Arch. bronconeumol. (Ed. impr.) ; 55(6): 306-311, jun. 2019. graf
Artigo em Inglês | IBECS | ID: ibc-181765

RESUMO

Introduction: The endotoxin lipopolysaccharide (LPS)-induced pulmonary endothelial barrier disruption is a key pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the molecular mechanisms underlying LPS-impaired permeability of pulmonary microvascular endothelial cells (PMVECs) are not fully understood. Methods: Rat PMVECs were isolated and monolayered cultured, then challenged with different doses of LPS (0.1 mg/L, 1 mg/L, and 10 mg/L). Trans-endothelial electrical resistance (TER) was utilized to measure the integrity of the endothelial barrier. Ras-related C3 botulinum toxin substrate 1 (Rac1) activity and the phosphorylation of Ezrin/Radixin/Moesin proteins (ERM) were assessed by pulldown assay and Western Blotting. Small interfering RNA (siRNA) inhibition of Rac1 and Moesin were applied to evaluate the effect of PMVEs permeability and related pathway. Results: LPS induced dose and time-dependent decreases in TER and increase in ERM threonine phosphorylation, while inactivated Rac1 activity in PMVEC. siRNA study demonstrated that both Rac1 and Moesin were involved in the mediation of the LPS-induced hyperpermeability in PMVECs monolayers, and Rac1 and Moesin could regulate each other. Conclusion: Phosphorylated ERM mediates LPS induced PMVECs permeability through negatively regulating Rac1 activity


Introducción: La disrupción de la barrera endotelial pulmonar inducida por endotoxina o lipopolisacárido (LPS) es un factor patogénico clave en la lesión pulmonar aguda (LPA) y el síndrome de distrés respiratorio agudo (SDRA). Sin embargo, los mecanismos que subyacen al empeoramiento de la permeabilidad de las células endoteliales de la microvasculatura pulmonar (PMVECs, por sus siglas en inglés) no se conocen. Métodos: Se aislaron y cultivaron en monocapa PMVEC de rata, y se expusieron a diferentes dosis de LPS (0,1, 1 y 10 mg/l). Se utilizó la resistencia eléctrica transendotelial (TER, por sus siglas en inglés) para medir la integridad de la barrera endotelial. Se analizó la actividad del sustrato 1 de la toxina botulínica C3 relacionado con Ras (Rac1) y la fosforilación de las proteínas erzina/raxidina/moesina (ERM) mediante ensayos pulldown y Western blot. Para evaluar la permeabilidad de las PMVEC y las vías relacionadas se inhibieron Rac1 y moesina mediante ARN pequeño de interferencia (siRNA, por sus siglas en inglés). Resultados: El LPS indujo una disminución dependiente de dosis y tiempo de la TER e incrementó la fosforilación en treonina de ERM, al mismo tiempo que inactivó a Rac1 en las PMVEC. El estudio con siRNA demostró que, tanto Rac1 como la moesina estaban implicadas en la mediación de la permeabilidad de las PMVEC en monocapa inducida por LPS, y que Rac1 y la moesina podrían regularse mutuamente. Conclusión: La fosforilación de ERM media la permeabilidad de las PMVECs inducida por LPS mediante la regulación negativa de la actividad de Rac1


Assuntos
Animais , Masculino , Ratos , Polissacarídeos/farmacologia , Fosforilação/fisiologia , Células Endoteliais/metabolismo , Permeabilidade Capilar/efeitos da radiação , Pulmão/irrigação sanguínea , Proteínas rac1 de Ligação ao GTP/metabolismo , Ratos Sprague-Dawley
17.
Sanid. mil ; 75(2): 98-101, abr.-jun. 2019.
Artigo em Espanhol | IBECS | ID: ibc-183712

RESUMO

El uso de perros detectores está ampliamente implantado entre las Fuerzas Armadas y Policiales de todo el mundo. Pero cada país, e incluso a menudo cada rama de los ejércitos, tienen un sistema de entrenamiento diferente para estos animales, lo que dificulta la tarea de establecer un estándar con el que medir sus resultados al desarrollar dicha práctica. A través de una revisión bibliográfica de estudios de etología canina, el autor trata de dirimir cuál es el mínimo porcentaje de detección que podemos esperar de un perro para decir que está correctamente entrenado, a fin de que se convierta en un estándar válido y eficaz por el que puedan medirse los resultados de los perros detectores de las Fuerzas Armadas


The use of detector dogs is widely implanted among the Armed Forces and Police around the world. But each country, and even often each branch of the military, has a different training system for these animals, making it difficult to establish a standard against which to measure their performance in developing such a practice. Through a literature review of canine ethology studies, the author attempts to determine what is the minimum percentage of detection we can expect from a dog to say that it is properly trained, so that it becomes a valid and effective standard by which to measure the results of detection dogs in the Armed Forces


Assuntos
Animais , Cães , Etologia/métodos , Aptidão , Testes de Aptidão , Etologia/instrumentação , Etologia/tendências
18.
Cir. plást. ibero-latinoam ; 45(2): 107-114, abr.-jun. 2019. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-184218

RESUMO

Introducción y objetivo. Las células madre son candidatas terapéuticas para una amplia gama de enfermedades. Resultan de gran interés en Cirugía Plástica para el tratamiento de heridas crónicas, transferencia de tejido adiposo y colgajos. El objetivo de este estudio es describir el método de aislamiento, cultivo y caracterización de células madre derivadas de tejido adiposo y cómo estas pueden precondicionarse con hipoxia y generar cambios in vitro en su capacidad proliferativa y migratoria. El trabajo es un complemento didáctico a otro publicado por nosotros en esta misma revista utilizando esta metodología en comparación al grupo de retardo de colgajo y grupo control en colgajos cutáneos aleatorizados en ratas. Métodos. Obtuvimos las células madre de tejido graso ínguino-abdominal de ratas adultas: 10 en el grupo de células madre derivadas de tejido adiposo y otras 10 en el grupo de células madre derivadas de tejido adiposo precondicionadas con hipoxia (2% O2 y 5% CO2). Realizamos análisis morfológico directo y con inmunofluorescencia con el marcador vimentina y CD90 y estudio de proliferación y migración celular in vitro. Resultados: Utilizamos en promedio 1.64 +/- 1.13 gr de tejido adiposo en el grupo sin precondicionamiento y 0.93 +/- 0.34 gr en el grupo con precondicionamiento con hipoxia para el aislamiento. Las células madre derivadas de tejido adiposo precondicionadas con hipoxia presentaron un aumento de la capacidad migratoria a las 24 horas de 2.44 +/- 0.85 mm frente a 2.24 +/- 0.82 mm (p ≤ 0.01) y proliferativa 5.42 x105 +/- 1.03 x105 céls/ml frente a 3.26 x105 +/- 8.61 x104 céls/ml) (p ≤ 0.001) de forma significativa en comparación a las sin precondicionamiento. Conclusiones. Describimos en detalle un método de precondicionamiento de células madre mediante hipoxia. Logramos potenciar el efecto de las células madre aumentando en forma significativa su capacidad migratoria y proliferativa de forma precoz


Background and objective. Stem cells are therapeutic candidates for a wide range of diseases. They are of great interest in Plastic Surgery for the management of chronic wounds, adipose tissue transfer and flaps. The objective of this study is to describe the method of isolation, culture and characterization of stem cells derived from adipose tissue and how these can be preconditioned with hypoxia and generate in vitro changes in their proliferative and migratory capacity. This study is a didactic supplement to a paper published by us in this same journal using this methodology in comparison to the group of flap delay and control group in skin flaps randomized in rats. Methods. Stem cells were obtained from inguinal-abdominal fatty tissue of adult rats: 10 in the group of stem cells derived from adipose tissue and another 10 in the group of stem cells derived from adipose tissue preconditioned with hypoxia (2% O2 and 5% CO2). Direct morphological analysis was carried out and with immunofluorescence (vimentin and CD90 marker). Study proliferation and in vitro cell migration was performed. Results. An average of 1.64 +/- 1.13 gr of adipose tissue of the inguinoabdominal area was used in the group of stem cells without preconditioning and 0.93 +/- 0.34 gr. in the group with hypoxic preconditioning for the isolation. Stem cells derived from adipose tissue preconditioned with hypoxia showed an increase in migratory capacity at 24 hours of 2.44 +/- 0.85 mm v/s 2.24 +/- 0.82 mm (p ≤ 0.01) and proliferative of 5.42 x105 +/- 1.03 x105 cells / ml v/s 3.26 x 105 +/- 8.61 x104 cells / ml) (p ≤0.001) significantly compared to those without preconditioning. Conclusions. A method of preconditioning stem cells by hypoxia is described in detail. It is possible to enhance the effect of the stem cells, significantly increasing their early migratory and proliferative capacity


Assuntos
Animais , Masculino , Ratos , Células-Tronco/citologia , Tecido Adiposo/citologia , Hipóxia/veterinária , Tecido Adiposo/enzimologia , Movimento Celular , Ratos Sprague-Dawley , Técnicas In Vitro/instrumentação , Tecido Adiposo/metabolismo , Imuno-Histoquímica , Proliferação de Células , Imunofluorescência , Neovascularização Fisiológica
19.
Eur. j. anat ; 23(3): 201-213, mayo 2019. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-182981

RESUMO

Cisplatin is a potent chemotherapeutic agent used to treat a variety of cancers such as ovarian, uterine, and bladder. The major limiting side effect of cisplatin is its hepatotoxicity. The possible mechanism of cisplatin hepatotoxicity was due to the affection of oxidant-antioxidant system. Platelet-rich plasma (PRP) has a powerful therapeutic option for its ability to deliver a great variety of biologically active GFs to the site of injury. PRP has grown as an attractive biologic instrument in regenerative medicine for its powerful healing properties. It is considered as a source of growth factors that may induce tissue repairing and improve fibrosis. This product has proven its efficacy in multiple studies, but its effect on cisplatin-induced hepatotoxicity has not yet been elucidated. The present study was designed to analyze the therapeutic role of PRP in cisplatin-induced hepatotoxicity. 30 adult male adult male albino rats were used in the present study divided into 3 groups (control group, cisplatin-treated group and PRP-treated group). By the end of the experimental period, blood samples were collected for measurement of serum AST, ALT and ALP enzymes; then the rats were sacrificed by cervical dislocation. Fresh liver parts were used to measure the oxidative markers in liver homogenates, while other parts were processed and subjected for histopathological and histomorphometric and immunohistochemical analyses for VEGF, Caspase 3 expression of the different experimental groups. The statistical study was done for the resultant data. Group II (Cisplatin-treated group) showed marked pathological hepatic changes; loss of architecture, congested dilated sinusoids lined by darkly stained pyknotic Kupffer cells; and hepatocytes nuclei were pyknotic and karyolitic. Dilated congested portal vein, interstitial acidophilic exudate, marked polymorphic cellular infiltration. There were increased collagen fibers deposition, a weak positive PAS reaction, strong positive caspase 3 reactions and strong positive VEGF reaction. Also, there were a marked increase in hepatic enzymes, MDA levels and a marked decrease in GSH level. Treatment with PRP in Group III revealed improvement of the hepatic parenchymal architecture with strong PAS reaction and minimal collagen fibers deposition. Weak positive caspase immunoreaction and strong positive VEGF reaction were noticed. Also, there was a marked improvement in the parameter of hepatic enzymes, MDA and GSH level comparable with the control group. It is concluded that PRP could ameliorate the liver against cisplatin-induced hepatotoxicity


No disponible


Assuntos
Animais , Ratos , Terapêutica , Plasma Rico em Plaquetas/química , Cisplatino/toxicidade , Fator Apoptótico 1 Ativador de Proteases/toxicidade , Caspase 3/toxicidade , Fator Apoptótico 1 Ativador de Proteases/efeitos adversos , Plasma Rico em Plaquetas/enzimologia , Imuno-Histoquímica , Receptores de Fatores de Crescimento do Endotélio Vascular/administração & dosagem , Projetos de Pesquisa , Fígado/química , Fígado/enzimologia , Biomarcadores , Análise de Variância
20.
Eur. j. anat ; 23(3): 215-221, mayo 2019. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-182982

RESUMO

Monosodium glutamate (MSG), known as AJI-NO-MOTO, is the sodium salt of glutamic acid. Glutamate is one of the most common amino acids found in nature, and is the main component of many proteins and peptides of most tissues. Glutamate is also produced in the body and plays an essential role in human metabolism. MSG is commonly used as a flavor enhancer. Lycopene (LPN) is a member of carotenoids, and it is an antioxidant substance found in tomato, and in other red fruits, and vegetables. The present study aimed to investigate the potential protective effects of LPN on MSG-induced nephrotoxicity in adult male albino rats. 40 adult male albino rats were divided into 4 groups with 10 rats in each group. Group I (control group). Group ІІ animals received lycopene orally in a dose of 4 mg/kg b.w. per day for 14 days. Group ІІI animals received MSG subcutaneously in a dose of 4 mg/g b.w. per day for 14 days. Group ІV animals received MSG Plus LPN. At the end of the experiment, kidney specimens were processed for histopathological, immunohistochemistry and biochemical studies. Administration of lycopene decreased elevated serum creatinine, blood urea nitrogen and immunoexpression of the proapoptotic protein (Bax), induced by MSG. It increased the immunoexpression of the antiapoptotic protein (Bcl2). It also ameliorated the morphological changes induced by MSG. MSG has toxic effects on the kidneys as indicated by biochemical, histological and immunohistochemical results. Lycopene has protective effects against MSG-nephrotoxicity by reducing elevated serum creatinine, blood urea nitrogen, kidney damage, and apoptosis


No disponible


Assuntos
Animais , Ratos , Licopeno/administração & dosagem , Glutamato de Sódio/toxicidade , Fígado/efeitos dos fármacos , Córtex Renal/anatomia & histologia , Imuno-Histoquímica , Fígado/lesões , Projetos de Pesquisa , Rim/efeitos dos fármacos , Fotomicrografia , Peso Corporal
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA