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1.
Int. microbiol ; 22(1): 121-130, mar. 2019. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-184820

RESUMO

The aim of the present study was to isolate novel lactic acid bacteria (LAB) from hairy vetch forage crop and characterize their probiotic and fermentative potential for preparing Korean cabbage kimchi. First, functional characterization of isolated strains such as antagonistic property, auto-aggregation, antibiotic susceptibility, and extracellular enzyme production was performed. The isolated Lactobacillus plantarum KCC-41 strain was able to inhibit pathogenic fungal spore formation. It showed susceptibility to common commercial antibiotics drugs. The selected LAB strain was then subjected to microencapsulation with alginate biopolymer. Its ability to survive in in vitro simulated gastro-intestinal fluid was evaluated. It was also used in the fermentation of cabbage kimchi samples. The encapsulated KCC-41 strain could effectively lead to kimchi fermentation in terms of reducing its pH and dominating bacterial count. It also significantly increased organic acid production than non-encapsulated LAB (KCC-41) for cabbage kimchi samples


No disponible


Assuntos
Brassica/microbiologia , Células Imobilizadas/metabolismo , Microbiologia de Alimentos , Probióticos/metabolismo , Endófitos/isolamento & purificação , Lactobacillus plantarum/isolamento & purificação , Vicia/microbiologia , Composição de Medicamentos , Endófitos/metabolismo , Lactobacillus plantarum/metabolismo
2.
Eur. j. anat ; 23(2): 121-129, mar. 2019. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-182422

RESUMO

Polycystic ovary syndrome (PCOS) is one of the most common causes of infertility. The aim of this study was to investigate the protective role of broccoli extract on estradiol valerate (EV)-induced PCOS in female rats. Forty adult female rats were divided into four main groups; control, broccoli-treated, EV, single intramuscular injection of 16mg/kg)-treated, EV+broccoli (1 g/kg/day)-treated groups. The protected rats were treated orally by gastric tube daily for 4 weeks. At the end of the experiment, blood samples were collected and the ovary were subjected to histological and immunohistochemical analyses. EV treated group exhibited the characteristic features of PCOS. Disturbed ovarian cyclicity in addition to histopathological alterations, including decreased number of healthy follicles and corpora lutea, increased degenerated, cystic follicles and increased collagen fiber deposition were detected by light microscopic studies. Moreover, increased immune-reactivity for iNOS and altered proliferation index were observed by immunohistochemical assessments. Co-adminis-tration of broccoli extract improved EV-induced PCOS in rat model. In conclusion, broccoli may be an effective therapeutic candidate for the treatment of PCOS


No disponible


Assuntos
Animais , Ratos , Síndrome do Ovário Policístico/induzido quimicamente , Síndrome do Ovário Policístico/veterinária , Estradiol/efeitos adversos , Brassica/efeitos adversos , Antioxidantes/efeitos adversos , Ratos Sprague-Dawley , Análise de Variância , Corpo Lúteo/anatomia & histologia
3.
Nutr. hosp ; 33(2): 373-378, mar.-abr. 2016. tab
Artigo em Inglês | IBECS | ID: ibc-153315

RESUMO

The effect of a cooking treatment (boiling in water at 100 ºC for 10 min) on antioxidant composition and sensorial quality of cauliflower that has been previously cut, packaged in a modified atmosphere (5.7-2.3% CO2 and 17.6-19.7% O2 ) and stored at 5 ºC for up to 13 days has been studied in comparison with cauliflower at harvest day. An increase of ascorbic acid (18%), phenolics (74%) and antioxidant activity (21%) has been observed in stored cauliflower. But the cooking treatment has produced a loss of these compounds by leaching. These losses have been greater for stored cauliflower than for the initial one at harvest day (19% and 13% respectively for ascorbic acid and 31% and 28% respectively for phenolics). However, no variations in antioxidant activity by cooking effect were observed at harvest day, while variations were by 16% in stored cauliflower. As a result, the antioxidant activity of stored and cooked cauliflower became similar to that of the fresh cauliflower at harvest day. The storage of cauliflower has produced some changes of color (an increase in b* parameter from 19 to 20.9) and texture (an increase in shear force from 1948 N to 2375 N). The sensorial acceptability for color and taste of cooked stored cauliflower has been lower than that of the cooked cauliflower at harvest day, but inside acceptable levels. However, the increase in hardness in raw stored cauliflower was not detected by judges in cooked cauliflower. A small incidence of molds on stored cauliflower at 13 days has been observed, thus a washing treatment is recommended (AU)


Se ha estudiado el efecto de la cocción en agua sobre la capacidad antioxidante y calidad sensorial de coliflor que previamente ha sido cortada, envasada en atmósfera modificada (5,7-2,3% de CO2 y 17,6-19,7% de O2 ) y almacenada a 5 ºC durante 13 días, comparándola con la coliflor recién recolectada. Se ha observado un aumento de ácido ascórbico (18%), polifenoles (74%) y actividad antioxidante (21%) en la coliflor almacenada cruda. La cocción produjo pérdidas de estos compuestos por lixiviación, que fueron mayores en la coliflor almacenada respecto a la inicial (19% y 13% respectivamente para el ácido ascórbico y 31% y 28% respectivamente para los polifenoles. Sin embargo, no se observaron variaciones en la actividad antioxidante de la coliflor inicial cocida respecto a la coliflor inicial fresca, mientras que en la coliflor almacenada dicha variación fue del 16%. Como resultado, la actividad antioxidante de la coliflor almacenada y cocida ha resultado similar a la de la coliflor inicial fresca. El almacenamiento produjo pequeños cambios en color (un aumento del parámetro b* de 19 a 20,9) y de la textura (un aumento de la fuerza de cizallamiento de 1948 N a 2375 N). La valoración sensorial del color y sabor de la coliflor cocida almacenada fue inferior al de la coliflor cocida inicial, pero dentro de niveles aceptables. El aumento de la dureza en la coliflor fresca almacenada no fue detectado por el panel de catadores en la coliflor cocida. Se observó una ligera incidencia de mohos en la coliflor almacenada 13 días, por lo que se recomienda realizar un tratamiento de lavado (AU)


Assuntos
Humanos , Masculino , Feminino , Análise de Alimentos/métodos , Antioxidantes/isolamento & purificação , Ácido Ascórbico/isolamento & purificação , Brassica , Qualidade dos Alimentos , Armazenamento de Alimentos , Manipulação de Alimentos , Culinária
4.
Nutr. hosp ; 31(2): 559-569, feb. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-133441

RESUMO

Introduction: Sulforaphane (SFN) is an isothiocyanate derived from glucoraphanin (GRA), which is found in great amounts especially in broccoli. Its consumption has been reported to be associated with a lower risk of myocardial infarction and cancer development. Additionally, its effects have been studied in neurodegenerative diseases, diabetes, and atherosclerosis, most of the times using animal models and cell cultures. Objectives: Given the promising results of SFN, this review aimed to investigate evidence documented in human intervention studies with broccoli, GRA and SFN. Methods: A search was performed on PubMed and Virtual Health Library databases by two independent researchers using the descriptors 'broccoli' or 'glucoraphanin' or 'sulforaphane', which should appear on the study’s title or abstract. This review included randomized linical trials performed in humans that were published in English and Portuguese from 2003 to 2013 and that considered clinical and molecular parameters of cell damage as outcomes of interest. Results: Seventeen studies were selected, and the predominant type of intervention was broccoli sprouts. More consistent results were obtained for the clinical parameters blood glucose and lipid profile and for molecular parameters of oxidative stress, indicating that there was an improvement in these parameters after intervention. Less solid evidence was found with regard to decreased inflammation, Helicobacter pylori colonization, and protection against cancer. Conclusion: Although being relevant, the evidence for the use of broccoli, GRA and SFN in humans are limited; Introduction: Sulforaphane (SFN) is an isothiocyanate derived from glucoraphanin (GRA), which is found in great amounts especially in broccoli. Its consumption has been reported to be associated with a lower risk of myocardial infarction and cancer development. Additionally, its effects have been studied in neurodegenerative diseases, diabetes, and atherosclerosis, most of the times using animal models and cell cultures. Objectives: Given the promising results of SFN, this reviewaimed to investigate evidence documented in human intervention studies with broccoli, GRA and SFN. Methods: A search was performed on PubMed and Virtual Health Library databases by two independent researchers using the descriptors 'broccoli' or 'glucoraphanin' or 'sulforaphane', which should appear on the study’s title or abstract. This review included randomized clinical trials performed in humans that were published in English and Portuguese from 2003 to 2013 and that considered clinical and molecular parameters of cell damage as outcomes of interest. Results: Seventeen studies were selected, and the predominanttype of intervention was broccoli sprouts. More consistent results were obtained for the clinical parameters blood glucose and lipid profile and for molecular parameters of oxidative stress, indicating that there wasan improvement in these parameters after intervention. Less solid evidence was found with regard to decreased inflammation, Helicobacter pylori colonization, and protection against cancer. Conclusion: Although being relevant, the evidence for the use of broccoli, GRA and SFN in humans are limited thus, further intervention studies are needed to evaluate outcomes more consistently and reach better grounded conclusions (AU)


Introducción: El sulforafano (SFN) es un isotiocianato derivado de la glucorafanina (GRA), encontrada en gran cantidad especialmente en el brócolis. Su consumo está asociado a un menor riesgo de infarto del miocardio y de cáncer. Además, sus efectos están siendo estudiados en enfermedades neurodegenerativas, diabetes y aterosclerosis, casi siempre utilizando modelos animales y cultivos celulares. Objetivos: Debido a los resultados prometedores del compuesto SFN, esta revisión buscó investigar evidencias ya documentadas en intervenciones con brócoli, GRA y SFN en humanos. Métodos: Se realizó una búsqueda en las bases de datos PubMed y Biblioteca Virtual en Salud, por dos investigadores independientes, utilizando los descriptores 'broccoli' o 'glucoraphanin' o 'sulforaphane', que debían constar en el título o resumen del trabajo. Se incluyeron ensayos clínicos randomizados realizados en humanos, publicados en inglés y portugués entre 2003 y 2013, y que consideraron como desenlaces de interés parámetros clínicos y moleculares de daño celular. Resultados: Se seleccionaron 17 estudios y el tipo de intervención predominante fueron brotes de brócoli. Los resultados más consistentes fueron obtenidos con los parámetros clínicos glicemia y perfil lipídico y los parámetros moleculares de estrés oxidativo, que presentaron mejora después de la intervención. Se encontraron evidencias menos sólidas respeto a la disminución de la inflamación, de la colonización por Helicobacter pylori y protección contra cáncer. Conclusión: Aunque relevantes, las evidencias del uso Correspondence: Carolina Guerini de Souza. de brócoli, GRA y SFN en humanos son limitadas, siendo necesarios más estudios de intervención para avaluar los desenlaces de forma más consistente y producir conclusiones mejor fundamentadas (AU)


Assuntos
Humanos , Anticarcinógenos/uso terapêutico , Brassica , Glucosinolatos/uso terapêutico , Imidoésteres/uso terapêutico , Isotiocianatos/uso terapêutico , Infarto do Miocárdio/prevenção & controle , Neoplasias/prevenção & controle , Dieta
5.
Gac. sanit. (Barc., Ed. impr.) ; 17(6): 512-514, nov. 2003.
Artigo em Espanhol | IBECS | ID: ibc-28722

RESUMO

El objetivo de este artículo es dar a conocer las decisiones tomadas por el Comité de Ética del Instituto de Salud Carlos III para el Síndrome del Aceite Tóxico en relación con el desarrollo de proyectos de investigación en los que se podían utilizar muestras recogidas con anterioridad y para cuyo uso no se disponía del consentimiento de los pacientes, sobre una situación muy común en el ámbito de la investigación biomédica. A partir del proceso de debate acerca de la idoneidad ética de la utilización secundaria de estas muestras, se llegó a la conclusión de que en los estudios prospectivos se debe solicitar expresamente y por escrito el consentimiento del participante en el estudio para conservar las muestras y que éstas puedan ser utilizadas en investigaciones futuras, estableciendo con el donante los límites de su utilización (AU)


Assuntos
Humanos , Brassica , Doenças Raras , Comitês de Ética em Pesquisa , Pesquisa Biomédica , Óleos Vegetais , Espanha , Síndrome
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