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Allergol. immunopatol ; 47(1): 73-78, ene.-feb. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-180775


Background: Serum IgE evaluation of peanut, hazelnut and walnut allergens through the use of component-resolved diagnosis (CRD) can be more accurate than IgE against whole food to associate with severe or mild reactions. Objectives: The aim of the study was to retrospectively define the level of reaction risk in children with peanut, hazelnut and walnut sensitization through the use of CRD. Methods: 34 patients [n = 22 males, 65%; median age eight years, interquartile range (IQR) 5.0-11.0 years] with a reported history of reactions to peanut and/or hazelnut and/or walnut had their serum analyzed for specific IgE (s-IgE) by ImmunoCAP(R) and ISAC(R) microarray technique. Results: In children with previous reactions to peanut, the positivity of Arah1 and Arah2 s-IgE was associated with a history of anaphylaxis to such food, while the positivity of Arah8 s-IgE were associated with mild reactions. Regarding hazelnut, the presence of positive Cora9 and, particularly, Cora14 s-IgE was associated with a history of anaphylaxis, while positive Cora1.0401 s-IgE were associated with mild reactions. Concerning walnut, the presence of positive Jug r 1, Jug r 2, Jug r 3 s-IgE was associated with a history of anaphylaxis to such food. ImmmunoCAP® proved to be more useful in retrospectively defining the risk of hazelnut anaphylaxis, because of the possibility of measuring Cor a14 s-IgE. Conclusions: Our data show that the use of CRD in patients with allergy to peanut, hazelnut and walnut could allow for greater accuracy in retrospectively defining the risk of anaphylactic reaction to such foods

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Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Anafilaxia/epidemiologia , Hipersensibilidade Alimentar/diagnóstico , Imunoglobulina E/sangue , Alérgenos/imunologia , Anafilaxia/etiologia , Arachis/imunologia , Hipersensibilidade Alimentar/complicações , Imunização , Itália/epidemiologia , Estudos Retrospectivos , Juglans/imunologia
Allergol. immunopatol ; 44(6): 524-530, nov.-dic. 2016. graf, tab, ilus
Artigo em Inglês | IBECS | ID: ibc-157873


Background: Peanut allergies are common and can be life-threating for sensitised individuals. Peanut allergens share significant amino acid homology with those of other legumes and tree nuts, but their cross-reactivity still remains unclear. Objective: We sought to determine the clinical significance of the cross-reactivity of peanut allergens with those of walnut and soybean. Methods: Pooled sera from eight subjects with both peanut and walnut specific IgE were investigated in an inhibition test. After the sera were incubated with either peanut or walnut protein extracts, the quantity of IgE antibodies against the peanut and walnut was measured using an immunoCAP test. Likewise, pooled sera from 18 subjects with both peanut and soybean specific IgE antibodies were incubated with either peanut or soybean protein extracts and evaluated with a peanut and soybean immunoCAP test. SDS-PAGE and immunoblotting were also performed with peanut, walnut and soybean protein extracts and relevant sera. Results: Peanut specific IgE was inhibited up to 20% and 26% by walnut and soybean protein extracts, respectively. In reverse, walnut and soybean specific IgE were inhibited up to 21% and 23% by peanut protein extracts, respectively. In the immunoblot analysis, pooled serum from the subjects with peanut specific IgE antibodies reacted with walnut protein extracts significantly. Conclusion: Although the clinical significance of the cross-reactivity of peanut specific IgE with walnut and soybean protein extracts has not been established, we believe that individuals who are allergic to peanuts need to be cautious about consuming walnuts and soybeans (AU)

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Humanos , Criança , Hipersensibilidade Alimentar/imunologia , Hipersensibilidade Imediata/imunologia , Apresentação Cruzada/imunologia , Soja/efeitos adversos , Arachis/efeitos adversos , Hipersensibilidade a Amendoim/imunologia , Juglans/efeitos adversos , Hipersensibilidade a Noz/imunologia
J. investig. allergol. clin. immunol ; 26(1): 31-39, 2016. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-150187


Background: Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy. Objective: To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy. Methods: sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cor a 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges. Results: Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP-sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P<.05). Similar rates of Cor a 8 and Jug r 3 sensitization were detected by both techniques. Conclusions: The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved (AU)

Introducción: La utilidad clínica del diagnóstico por componentes no ha sido evaluada en el estudio de la alergia a frutos secos (FS). Objetivo: Evaluar la capacidad diagnóstica de una micromatriz comercial de proteínas alergénicas en la alergia a cacahuete, avellana y nuez. Métodos: Se determinó la sIgE en pacientes alérgicos a FS mediante la micromatriz ISAC 112, e ImmunoCAP en los pacientes con sIgE negativa frente a los componentes de ISAC. Además, se realizó ImmunoCAP frente a Ara h 9, Cor a 8 y Jug r 3 en un subgrupo de pacientes sensibilizados a LTP. La sIgE detectada por ImmunoCAP fue comparada con los rangos de ISAC. Resultados: La mayoría de los alérgicos a cacahuete (66,7%), avellana (80,5%) y nuez (84%) estaba sensibilizados a su LTP. Sin embargo, no se detectó sIgE frente a los componentes de ISAC en el 33,3% de alérgicos a cacahuete, 13,9% de alérgicos a avellana y 13,6% de los alérgicos a nuez. El ImmunoCAP permitió detectar sIgE a Ara h 9 en 61,5%, Cor a 8 en 60% y Jug r 3 en 83,3% de los ISAC negativo. En el subgrupo LTP, ImmunoCAP (94,4%) fue superior a ISAC (72,2%) en la detección de sIgE a Ara h 9 (p<0,05). La sIgE frente a Cor a 8 y Jug r 3 fue detectada de forma similar por ambas técnicas. Conclusiones: La micromatriz ISAC es adecuada para el diagnóstico de alergia a avellana y nuez. La sensibilidad del componente Ara h 9 de ISAC debe ser mejorada (AU)

Humanos , Masculino , Feminino , Adolescente , Hipersensibilidade a Noz/imunologia , Arachis/imunologia , Hipersensibilidade a Amendoim/imunologia , Corylus/imunologia , Hipersensibilidade/diagnóstico , Hipersensibilidade/imunologia , Testes Imunológicos/instrumentação , Testes Imunológicos/métodos , Testes Imunológicos , Técnicas Imunológicas/métodos , Testes Imunológicos/classificação , Testes Imunológicos/estatística & dados numéricos , Testes Imunológicos/normas , Técnicas Imunológicas/instrumentação , Técnicas Imunológicas/normas , Técnicas Imunológicas
Nutr. hosp ; 29(5): 1024-1032, mayo 2014. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-143840


Background: Evidences suggest that nuts consumption can improve energy metabolism. Purpose: This study aimed to compare the effects of acute ingestion of high-oleic and conventional peanuts on appetite, food intake, and energy metabolism in overweight and obese men. Methods: Seventy one subjects (29.8 ± 2.4 kg/m2) were assigned to the groups: control (CT, n = 24); conventional peanuts (CVP, n = 23); high-oleic peanuts (HOP, n = 24). Subjects consumed 56 g of peanuts (CVP and HOP) or control biscuits (CT) after overnight fasting. Thereafter, energy metabolism was evaluated over 200 minutes, during which diet-induced thermogenesis (DIT) and substrate oxidation were analyzed. Appetite sensation was recorded for 3 hours. Statistical analyses were performed using the SAS software considering 5% as the significance level. Results: Postprandial energy expenditure and DIT were significantly higher in HOP than in CVP. Substrate oxidation did not differ between groups. Only HOP presented score below 100 indicating incomplete compensation. CT and CVP showed a complete caloric compensation (scores > 100). Regarding appetite sensation, CVP group felt less «full» than HOP and CT. After 3 hours, satiety score of CVP returned to baseline, whereas HOP and CT remained significantly higher. Hunger scores returned to baseline in CVP and CT and they were maintained significantly lowered in HOP. Conclusion: High-oleic peanuts contributed to higher DIT, higher sensation of fullness and incomplete compensation for energy intake compared to conventional peanuts and may be useful to dietary intervention to reduce body weight (AU)

Antecedentes: Las pruebas sugieren que el consumo de frutos secos puede mejorar el metabolismo energético. Propósito: Este estudio tenía por finalidad comparar los efectos de la ingesta aguda de cacahuetes con alto contenido en oleico y cacahuetes convencionales sobre el apetito, el consumo de alimentos y el metabolismo energético in hombres con sobrepeso y obesos. Métodos: Se distribuyó a 71 individuos (29,8 ± 2,4 kg/m2) a los grupos: control (CT, n = 24); cacahuetes convencionales (CVP, n = 23); cacahuetes con alto contenido en oleico (HOP, n = 24). Los individuos consumieron 56 g de cacahuetes (CVP y HOP) o control (CT) tras un ayuno nocturno. Posteriormente, se evaluó el metabolismo energético a lo largo de 200 minutes, durante los cuales se analizaron la termogénesis inducida por la dieta (TID) y la oxidación de sustratos. La sensación de apetito se registró durante 3 horas. Se realizaron los análisis estadísticos con el programa SAS considerando un nivel de significación del 5%. Resultados: El consumo de energía posprandial y la TID fueron significativamente superiores en el HOP que el CVP. La oxidación de sustratos no difirió entre los grupos. Sólo el HOP presentó una puntuación por debajo de 100, lo que indicaba una compensación incompleta. El CT y el CVP mostraron una compensación calórica completa (puntuaciones > 100). Con respecto a la sensación de apetito, el grupo CVP se mostró menos «lleno» que los grupos HOP y CT. A las 3 horas, la puntuación de saciedad del CVP volvió a la situación basal, mientras que en los grupos HOP y CT permanecía significativamente superior. Las puntuaciones de hambre volvieron a la situación basal in los grupos CVP y CT y se mantuvieron significativamente por debajo a las del grupo HOP. Conclusión: Los cacahuetes con alto contenido en oleico contribuyen a una mayor TID, mayor sensación de plenitud y una compensation incompleta del consumo de energía en comparación con los cacahuetes convencionales y pueden ser de ayuda como intervention dietética para disminuir el peso corporal (AU)

Humanos , Termogênese/fisiologia , Arachis , Obesidade/fisiopatologia , Sobrepeso/fisiopatologia , Ácidos Oleicos/farmacocinética , Metabolismo Energético/fisiologia , Estresse Oxidativo/fisiologia , Estudos de Casos e Controles , Dieta Redutora , Apetite/fisiologia
Nutr. hosp ; 28(1): 6-92, ene.-feb. 2013. tab
Artigo em Inglês | IBECS | ID: ibc-123113


Introduction: Prebiotic and food with functional properties are beneficial for consumers through prevention of many diseases. Aim: Verify the acceptance of handmade product (chocolate bar, soy sweet and sweet bread) formulated based on oil seeds (flaxseed, peanut and Brazil nut) and or fructooligosaccharides (FOS). Methods: Four samples of each handmade product were prepared adding different concentrations of oil seed and FOS. The sensory evaluation was performed by a sample of 373 consumers; 126, 121 and 126 tasters of chocolate bar, soy sweet and sweet bread, respectively, using a hedonic scale of nine points. The results were submitted to analysis of variance (ANOVA) and Tukey's test. Results and Discussion: Observing the trials averages, we inferred that samples of sweet bread with Brazil nut and/or FOS had the greater acceptance. However, all the samples are good market alternatives because they had presented averages between 6 and 9 points, and conferred accretion of omega-3 and omega-6 fatty acids, protein, fiber, antioxidant vitamins and minerals, as well as, phytochemicals, which plays an important role in health promotion. Conclusion: The handmade products formulated based on oil seeds and FOS had good acceptance and can improve the consumer dietary patterns. But, in order to prove the functionality of these products, new studies should be performed (AU)

Introducción: Los prebióticos y alimentos con propiedades funcionales proporcionan beneficios para la salud de los consumidores a través de la prevención de muchas enfermedades. Objetivo: Verificar la aceptación de productos artesanales (chocolate en barra, dulce de soja y pan dulce) formulados con nueces (linaza, maní y nueces de Brasil) y, o fructooligosacáridos (FOS). Métodos: Cuatro muestras de cada producto fueron preparados con adición de diferentes concentraciones de nueces y FOS. La evaluación sensorial se realizó mediante una muestra de 373 consumidores, con 126, 121 y 126 probadores para muestras de chocolate en barra, dulce de soja y pan dulce, respectivamente, utilizándose la escala hedónica de nueve puntos. Los resultados fueron sometidos a Análisis de Varianza (ANOVA) y el test de Tukey. Resultados y Discusión: Observándose las medias de los juzgamientos, se infiere que las muestras con mayor aceptación han sido de pan dulce con nueces de Brasil y, o FOS. Sin embargo, todas las muestras son buenas alternativas de mercado y se lo mostró un promedio de entre 6 y 9 puntos, más un aumento de ácidos grasos omega 3 y 6, proteínas, fibras, vitaminas, antioxidantes y minerales, así como fitoquímicos, los cuales desempeñan un papel importante en la promoción de la salud. Conclusión: Los productos artesanales formulados con oleaginosas y, o FOS tuvieron una buena aceptabilidad y pueden mejorar los hábitos alimentarios de los consumidores. Pero para probar la funcionalidad de estos productos, se necesitan nuevos estudios (AU)

Humanos , Oligossacarídeos/uso terapêutico , Frutose/uso terapêutico , Prebióticos , Alimento Funcional , Preferências Alimentares , Linho , Nozes , Cacau , Arachis , Comportamento Alimentar
Nutr. hosp ; 26(4): 745-751, jul.-ago. 2011. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-111147


Introduction: Few studies have evaluated the effect of nuts processing on the glycemic response and satiety. Objective: To evaluate the effect of peanut processing on glycemic response, and energy and nutrients intake. Method: Thirteen healthy subjects (4 men and 9women), with a mean age of 28.5 ± 10 years, BMI 22.7 ±2.5 kg/m², and body fat 23.7 ± 5.7% participated in this randomized crossover clinical trial. After 10-12 h of fasting, one of the following types of test meals were consumed: raw peanuts with skin (RPS), roasted peanuts without skin, ground-roasted peanuts without skin(GRPWS) or control meal. The test meals had the same nutrient composition, and were consumed with 200 ml of water in 15 minutes. Glycemic response was evaluated 2 hours after each meal. Energy and nutrients intake were assessed through diet records reflecting the habitual food intake and food consumption 24 hours after the ingestion of test meal. Result: The area under the glycemic response curve after GRPWS was lower (p = 0.02) the one obtained for RPS. There was no treatment effect on energy intake, macronutrients and fiber consumption after the test meal. Conclusion: The consumption of ground-roasted peanuts may favor the control and prevention of diabetes due to its reduction on postprandial glucose response. However, more prospective studies are needed to confirm this hypothesis (AU)

Introducción: Escasos estudios han evaluado el efecto del procesado industrial de los frutos secos sobre la respuesta glicérica y la saciedad. Objetivos: Evaluar el efecto del procesamiento de maní sobre la respuesta glicémica y la ingesta de energía y nutrientes. Métodos: Trece sujetos sanos (4 hombres y 9 mujeres),con una edad media de 28,5 ± 10 años, IMC 22,7 ± 2,5kg/m², y un porcentaje de grasa corporal de 23,7 ± 5,7% participaron en este ensayo clínico aleatorizado y cruzado. Tras 10-12 h de ayuno uno de los siguientes tipos de comidas test fueron consumidas: maní crudo con la piel(RPS), maní tostado sin piel, maní tostado y molido sin piel (GRPWS) o comida control. Las comidas test presentaban la misma composición nutricional, y fueron consumidas con 200 ml de agua en 15 minutos. Se evaluó la respuesta glucémica 2 horas después de cada una de las comidas. La ingesta de energía y nutrientes contenida en la toma alimentaria y las 24 horas posteriores a la comida test fueron determinadas mediante registros dietéticos. Resultados: El área bajo la curva de respuesta glicémica después de GRPWS fue menor (p = 0,02) que la de RPS. No hubo efecto de los tratamientos sobre la ingesta de energía, macronutrientes y fibra posterior a la comida test. Conclusión: El consumo de maní tostado y molido sin piel, al reducir la respuesta glucémica postprandial podría ser beneficioso para el control y prevención de la diabetes. Sin embargo son necesarios estudios de intervención a largo plazo que lo confirmen (AU)

Humanos , Ingestão de Alimentos/fisiologia , Índice Glicêmico/fisiologia , Arachis , Glicemia/análise , Diabetes Mellitus/prevenção & controle
Allergol. immunopatol ; 30(6): 338-341, nov. 2002.
Artigo em Inglês | IBECS | ID: ibc-21045


Background: Food allergy is quite common in children, but it usually trends to improve with ageing. When an individual has specific IgE to a large variety of foods (multifood allergy) the clinical picture may be of remarkable severity and the avoidance of the offending foods may lead to severe dietary impairment. We describe a case of a child with ascertained multifood allergy. Methods: The prick-by-prick testing with fresh foods and serum IgE tests were used to evaluate the patient's sensitivity to food allergens. The clinical effect of sensitizations were evaluated by DBPCFC which was carried out for egg, fish, peanuts, walnut, fig, asparagus, orange, chicory, medlar, peach, strawberry and cherry. Each challenge was performed on a separate day. Medical assistance and resuscitation facilities were available during the whole challenge procedures. Results: SPT with fresh food gave a 4 mm wheal for fig, asparagus, cherry and walnut; a 5 mm wheal for medlar and orange; a 6 mm wheal for chicory and strawberry; a 7 mm wheal for fish and peanuts; an 8 mm wheal for peach and a 9 mm wheal for egg. The RAST assay confirmed the presence of specific IgE to egg, fish, peanuts, walnut, fig, orange, strawberry, peach, and cherry. The total serum IgE was 730,6 kU/l. The DBPCFC was positive, at various degrees, for all foods tested according to skin sensitizations. Conclusions: The case herein described is a true multifood allergy, as confirmed by the DBPCFC. Multifood allergy is not common, but when present it can lead to severe dietary limitation (AU)

Antecedentes: La alergia a alimentos es muy común en niños y tiende a mejorar con la edad. Cuando un individuo presenta IgE específica frente a una gran variedad de alimentos (alergia alimentaria múltiple), su cuadro clínico puede ser de gravedad notable y el evitar los alimentos responsables de esa alergia, puede generar problemas dietéticos. En este estudio, se describe el caso de un niño con alergia alimentaria múltiple establecida. Métodos: Para evaluar la sensibilidad del paciente a alergenos de alimentos, se utilizó la prueba prick-prick con alimentos frescos y además, los valores de IgE en suero. La respuesta clínica a las sensibilizaciones, se evaluó por PPDCCP, el cual se llevó a cabo con huevo, pescado, cacahuete, nuez, higo, espárrago, naranja, escarola, níspero, melocotón, fresa y cereza. Cada prueba se realizó en un día diferente y, se dispuso siempre de asistencia médica y medidas de resucitación cardio-respiratoria. Resultados: El SPT con alimentos frescos, dio una pápula de 4 mm para higo, espárrago, cereza y nuez; de 5 mm, para níspero, y naranja; de 6 mm para escarola y fresa; de 7 mm para pescado y cacahuete; de 8 mm para melocotón y de 9 mm para huevo. El análisis de RAST confirmó la presencia de IgE específica frente a huevo, pescado, cacahuete, nuez, higo, naranja, fresa, melocotón y cereza. El resultado de IgE total en suero fue de 730,6 kU/l.El PPDCCP fue positivo, a varios niveles, para todos los alimentos probados en relación a las sensibilizaciones cutáneas. Conclusiones: El caso descrito en este artículo, es una alergia alimentaria múltiple como se confirmó por el PPDCCP. La alergia alimentaria múltiple, no es muy común, pero cuando se presenta, conlleva limitaciones importantes en la dieta (AU)

Animais , Pré-Escolar , Masculino , Humanos , Plantas , Urticária , Alimentos Marinhos , Arachis , Nozes , Especificidade de Anticorpos , Asma , Alérgenos , Imunoglobulina E , Ovos , Hipersensibilidade Alimentar , Gastroenterite , Peixes , Frutas , Testes Cutâneos
Allergol. immunopatol ; 29(2): 69-71, mar. 2001.
Artigo em Inglês | IBECS | ID: ibc-8445


Background: the sharon fruit is the edible fruit of the persimmon tree (Diospyros Kaki) which belongs to the Ebenaceae family. There are few references of allergic reaction to this fruit. We introduce a case of a 33-years old man with a anaphylactic reaction immediately after ingestion of sharon fruit. Methods: skin prick test (SPT) were performed by prick by prick with sharon fruit, legumes, vegetables, and fresh fruit. The test was considered positive when the average diameter of the wheal was > 3 mm. Serum-specific IgE was identified with use of the EIA, and SDS-PAGE Immunoblotting. Results: the skin by prick test with sharon fruit was positive in our case, and negative in five controls patients. Specific IgE in patient serum, assayed by EIA was positive for sharon fruit, but the results of immunoblotting were non-specific. Conclusion: we have introduced a cause of anaphylaxis by sharon fruit that suggest an IgE mediated hypersensitivity reaction (AU)

Antecedentes: el saroni es la fruta comestible del árbol Persimon (Diospyros kaki), perteneciente a la familia de las Ebenáceas. Existen pocas referencias de reacciones alérgicas a esta fruta. Presentamos el caso de un hombre de 33 años con una reacción anafiláctica inmediatamente después de comer un saroni.Métodos: se realizaron pruebas cutáneas por prick-by-prick con saroni, legumbres, verduras y frutas frescas. Se consideró la prueba positiva cuando el diámetro medio del habón fue > 3 mm. La IgE específica fue identificada por EIA Resultados: el prick-by-prick con saroni fue positivo en el paciente, y negativo en cinco controles. La determinación de IgE específica por EIA fue positiva para saroni, pero los resultados del immunoblotting fueron inespecíficos.Conclusión: en este caso de anafilaxia por saroni, el mecanismo probable parece ser una reacción de hipersensibilidad mediada por IgE (AU)

Adulto , Masculino , Humanos , Proteínas de Plantas , Arachis , Nozes , Angioedema , Grão Comestível , Anafilaxia , Alérgenos , Imunoglobulina E , Frutas , Hipersensibilidade Alimentar , Testes Cutâneos , Doenças Profissionais