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Ars pharm ; 59(3): 145-151, jul.-sept. 2018. graf, tab
Artigo em Inglês | IBECS | ID: ibc-177730


Objective: The study aimed to verify the hypoglycemic effect of Murraya koenigii (M. koenigii) and Catharanthus roseus (C. roseus) by using various in-vitro techniques. Method: The extracts were studied for their effects on glucose adsorption capacity, in-vitro glucose diffusion, in-vitro amylolysis kinetics and glucose transport across the yeast cells. Results: It was observed that the extracts of M. koenigii and C. roseus adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. There were no significant (p≤0.05) differences between their adsorption capacities. In the amylolysis kinetic experimental model the rate of glucose diffusion was found to be increased with time from 30 to 180 min and both the plant extracts exhibited significant inhibitory effects on the movement of glucose into external solution across the dialysis membrane as compared to control. The extracts also promoted glucose uptake by the yeast cells and the enhancement of glucose uptake was dependent on both the sample and glucose concentration. The extract of M. koenigii exhibited significantly higher (p≤0.05) activity than the extract of C. roseus at all concentrations used in the study. Our report suggests the mechanism(s) for the hypoglycemic effect of M. koenigii and C. roseus. Conclusion: The said effect was observed to be mediated by inhibiting alpha amylase, inhibiting glucose diffusion by adsorbing glucose and by increasing glucose transport across the cell membranes as revealed by in-vitro model of yeast cells. However, these effects need to be affirmed by using different in vivo models and clinical trials

Objetivo: El estudio tuvo como objetivo verificar el efecto hipoglucémico de Murraya koenigii (M. koenigii) y Catharanthus roseus (C. roseus) mediante el uso de diversas técnicas in vitro. Método: Los extractos se estudiaron por sus efectos sobre la capacidad de adsorción de glucosa, la difusión de glucosa in vitro, la cinética de amilolisis in vitro y el transporte de glucosa a través de las células de levadura. Resultados: se observó que los extractos de M. koenigii y C. roseus adsorbieron glucosa y la adsorción de glucosa aumentó notablemente con un aumento en la concentración de glucosa. No hubo diferencias significativas (p≤0.05) entre sus capacidades de adsorción. En el modelo experimental cinético de amilolisis, se encontró que la velocidad de difusión de glucosa aumentaba con el tiempo de 30 a 180 min y ambos extractos de planta exhibían efectos inhibitorios significativos sobre el movimiento de la glucosa hacia la solución externa a través de la membrana de diálisis en comparación con el control. Los extractos también promovieron la absorción de glucosa por las células de levadura y la mejora de la captación de glucosa dependió tanto de la muestra como de la concentración de glucosa. El extracto de M. koenigii exhibió una actividad significativamente mayor (p≤0.05) que el extracto de C. roseus en todas las concentraciones utilizadas en el estudio. Nuestro informe sugiere el mecanismo (s) para el efecto hipoglucemiante de M. koenigii y C. roseus. Conclusión: Se observó que dicho efecto estaba mediado por la inhibición de la alfa amilasa, la inhibición de la difusión de glucosa por la adsorción de glucosa y el aumento del transporte de glucosa a través de las membranas celulares según lo revelado por el modelo in vitro de células de levadura. Sin embargo, estos efectos deben ser afirmados mediante el uso de diferentes modelos in vivo y ensayos clínicos

Hipoglicemia/tratamento farmacológico , Hipoglicemiantes/análise , Hipoglicemiantes/farmacologia , Técnicas In Vitro , Murraya/química , Catharanthus/química , Glucose/farmacologia , Extratos Vegetais/farmacologia , alfa-Amilases/antagonistas & inibidores , alfa-Amilases/farmacologia
J. physiol. biochem ; 69(3): 459-466, sept. 2013.
Artigo em Inglês | IBECS | ID: ibc-121665


High intake of dietary fructose has been shown to exert a number of adverse metabolic effects in humans and experimental animals. The present study was proposed to elucidate the effect of Catharanthus roseus (C. roseus) leaf powder treatment on alterations in carbohydrate and lipid metabolisms in rats fed with high-fructose diet. Male Wistar rats of body weight around 180 g were divided into four groups, two of these groups (groups C and C+CR) were fed with standard pellet diet and the other two groups (groups F and F+CR) were fed with high-fructose (66 %) diet. C. roseus leaf powder suspension in water (100 mg/kg body weight/day) was administered orally to group C+CR and group F+CR. At the end of a 60-day experimental period, biochemical parameters related to carbohydrate and lipid metabolisms were assayed. C. roseus treatment completely prevented the fructose-induced increased body weight, hyperglycemia, and hypertriglyceridemia. Hyperinsulinemia and insulin resistance observed in group F was significantly decreased with C. roseus treatment in group F+CR. The alterations observed in the activities of enzymes of carbohydrate and lipid metabolisms and contents of hepatic tissue lipids in group F rats were significantly restored to near normal values by C. roseus treatment in group F+CR. In conclusion, our study demonstrates that C. roseus treatment is effective in preventing fructose-induced insulin resistance and hypertriglyceridemia while attenuating the fructose-induced alterations in carbohydrate and lipid metabolisms. This study suggests that the plant can be used as an adjuvant for the prevention and/or management of insulin resistance and disorders related to it (AU)

Animais , Ratos , Erros Inatos do Metabolismo dos Carboidratos/fisiopatologia , Transtornos do Metabolismo dos Lipídeos/fisiopatologia , Catharanthus , Frutose/metabolismo , Modelos Animais de Doenças , Substâncias Protetoras/farmacocinética , Extratos Vegetais/farmacocinética , Síndrome Metabólica/metabolismo
Eur. j. anat ; 14(3): 111-119, dic. 2010. ilus
Artigo em Inglês | IBECS | ID: ibc-107661


Botanical drugs are complementary therapies in the management and treatment of clinical conditions. This study was aimed at investigating the possible changes in the structural and functional entities of two vital organs, the liver and kidney, following oral administration of ethanolic leaf extracts of Catharanthus roseus. Thirty-two wistar rats were used for this study and were randomly assigned into three treatment (n=24) and control (n=8) groups. The animals in the treatment groups A, B, and C respectively- received 400 mg, 300 mg and 200 mg per kg body weight of Madagascar periwinkle extract for twenty-one days, while the animals in the control group (group D) received an equal amount of phosphate buffered saline (PBS). The administration was performed orally using an orogastric tube over twenty-one days (21d). Twenty-four hours after the last administration, all the animals were sacrificed by cervical dislocation. Laparatomy was performed and the liver and kidney excised, trimmed free of fat, and rinsed in cold phosphate buffered saline solution. The liver was quickly fixed in 10% formolsaline, while the kidney was fixed in Bouin’s fluid for histological processing. Blood samples collected from the abdominal aorta and portions of the liver stored at -20°C in the refrigerator were used for the biochemical analysis of kidney metabolites and liver enzymes respectively. It was observed that the activities of the kidney metabolites and liver enzymes following the administration of the ethanolic extract of C. roseus were statistically and significantly reduced in a dose-dependent pattern in all the experimental groups when compared with the control group. The results obtained from this study suggest that the oral administration of ethanolic extracts of C. roseus has no compromising effect on the kidney and liver and may enhance the functional features of the organs of Wistar rats to a greater extent (AU)

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Animais , Ratos , Catharanthus , Extratos Vegetais/farmacocinética , Fígado , Rim , Experimentação Animal