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1.
Allergol. immunopatol ; 37(4): 180-187, jul.-ago. 2009. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-72807

RESUMO

Background The present study was designed to explore the possible changes in endogenous hydrogen sulphide (H2S), a novel gasotransmitter, on the pathogenesis of allergic rhinitis (AR). Methods AR guinea pig model was established by nasal ovalbumin sensitisation. Guinea pigs were divided into four groups: Saline control, AR sensitised, sodium hydrosulphide (NaHS) treated, and propargylglycine (PPG) treated group. The frequency of sneezing and nose rubbing was recorded. Leukocyte infiltration in nasal lavage fluid (NLF) and plasma H2S level were measured. Expression of Cystathionine-â-synthase (CBS) and Cystathionine-ã-lyase (CSE) mRNA as H2S-producing enzymes in nasal mucosa was determined by real time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Results The frequency of sneezing and nose rubbing, and levels of leukocyte infiltration in NLF were higher than those of control (P<0.01), but plasma H2S in sensitised guinea pigs was lower than those of control (P<0.05). From the results of RT-PCR, it was found that the expression of CSE was higher than CBS in nasal mucosa, and in sensitised guinea pigs it was lower than that of control (P<0.05). NaHS successfully increased the level of H2S and alleviated the symptoms of AR accompanied by up-regulation of CSE as compared with AR group (P<0.05). PPG significantly suppressed the expression of CSE and decreased the H2S level, yet also aggravated the symptoms of AR. Conclusion H2S level may be negatively correlated with the process of inflammation and positively correlated with expression of CSE in nasal mucosa. The endogenous H2S pathway is down-regulated in AR (AU)


Assuntos
Animais , Rinite Alérgica Perene/fisiopatologia , Sulfeto de Hidrogênio/análise , Mucosa Nasal/fisiopatologia , /farmacocinética , Cistationina gama-Liase/farmacocinética , Suínos , Inflamação/fisiopatologia , Modelos Animais de Doenças , Reação em Cadeia da Polimerase
2.
Rev. toxicol ; 24(1): 45-47, 2007. tab
Artigo em Espanhol | IBECS | ID: ibc-75358

RESUMO

Un varón de 49 años de edad presentó una intoxicación aguda por sulfuro de hidrógeno mientras realizaba tareas de mantenimiento en una industria de refinado de hidrocarburos, sin utilizar ningún tipo de equipo de protección individual. A los seis meses de la intoxicación presenta secuelas neurológicas irreversibles de tipo cerebeloso y cognitivo, reuniendo además criterios diagnósticos del síndrome de fatiga crónica. El paciente ha quedado en una situación de invalidez absoluta para cualquier tipo de actividad laboral. Se destacan los aspectos preventivos de las intoxicaciones por sulfuro de hidrógeno, ya que ocasionan un número elevado de casos mortales y los pacientes que sobreviven pueden quedar con secuelas neurológicas irreversibles(AU)


A 49-year-old male presented acute hydrogen sulfide poisoning while carrying out maintenance work without any type of protective equipment in a hydrocarbon refining plant. Six months after the poisoning, the patient presents irreversible cerebellar and cognitive neurological sequelae and also fulfils the criteria for diagnosis of chronic fatigue syndrome. He has been declared permanently work-disabled. We stress the importance of preventive measures against hydrogen sulfide poisonings, a large percentage of which result in death, with surviving patients often suffering irreversible neurological sequelae(AU)


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Sulfeto de Hidrogênio/efeitos adversos , Sulfeto de Hidrogênio/análise , Sulfeto de Hidrogênio/toxicidade , Riscos Ocupacionais , Fatores de Risco , Síndrome de Fadiga Crônica/complicações , Síndrome de Fadiga Crônica/diagnóstico , Saúde do Trabalhador
3.
Rev. iberoam. micol ; 23(4): 224-232, dic. 2006. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-75395

RESUMO

El Chacolí de Vizcaya/Bizkaiko Txaxolina es un vino blanco característico del País Vaso con denominación de origen (BOPV 14/6/94). El objetivo del presente estudio fue seleccionar cepas de levaduras autóctonas para mejorar las condiciones de elaboración manteniendo las características propias de los vinos de esta región. Se aislaron levaduras identificadas como Saccharomyces bayanus durante las campañas 1996-1998 y se sometieron a un proceso selectivo en función de sus características enológicas y su comportamiento fermentativo. Tres de las cepas seleccionadas se inocularon a escala de bodega sobre mostos monovarietales de las dos variedades de uva aceptadas en esta denominación de origen, Hondarrabi Zuri y Folle Blanche. La implantación de las cepas inoculadas en las respectivas vinificaciones fue controlada mediante el análisis del polimorfismo de restricción ADN mitocondrial (REAmt) con la comparación con la enzima Alul, dada su especificidad, rapidez y sencillez tecnológica en comparación con otras técnicas de tipificación molecular utilizadas también en este estudio: cariotipificación cromosómica mediante electroforesis en campo pulsado, Random Amplified Polymorphic DNA-pCR (RAPD-pCR) y análisis del polimorfismo de restricción originado por la enzima Sfil de corte infrecuente (REA infrecuente). Este estudio ha demostrado que cepas con comportamientos fenotípicos diferentes presentan los mismos patrones de restricción con REAmt, pero pueden ser diferenciadas con otras técnicas aplicadas en este estudio, como RAPD-PCR, que pese a su baja reproductibilidad pueden ser una herramienta complementaria a REAmt. Nuestros resultados demuestran que, a pesar de utilizar cepas autóctonas seleccionadas, la inoculación de una caldo con una cepa no es garantía de que doina y dirija la fermentación del mosto, ya que una misma cepa puede imponerse o no dependiendo del tipo de mosto y de la campaña de que se trate. De las levaduras estudiadas, la cepa 2 fue la que mejores resultados proporcionó tanto en cata como en implantación, por lo que podría ser utilizada para la producción de Chacolí de Vizcaya con fines comerciales, sobre todo de mostos procedentes de Folle Blanche(AU)


The white wine Chacolía de Vizcaya/Bizkaiko Txakolina is characteristic from The Basque Country region and regulated under Appellation Contrôlée standards (BOPV 14/6/94). The objective of this study was the identification and selection of autochthonous yeast strains, to improve the conditions used to maintain the typical characteristics of this region wines. Yeasts identified as Saccharomyces bayanus isolated around these fields from 1996 to 1998, were subjected to a selective procedure based on enological characteristics and fermentative behaviour. Three of the selected strains were used to inoculate, at winery scale, two grape juice varieties accepted by the Appellation Contrôlée (Hondarrabi Zuri and Folle Blanche). The inoculated strains on the respective vinifications was followed by restriction fragment length polymorphism of mitochondrial DNA (REAmt) method with AluI enzyme, due to their specificity, short outcome, and technological simplicity compared with other molecular typing methods such as: chromosomal karyotyping analyzed by pulsed field gel electrophoresis, Random Amplified Polymorphic DNA-PCR (RAPD-PCR) and restriction fragment length polymorphism using the infrequently cutting enzyme SfiI (REA infrequent). This study demonstrated that strains with different phenotypic traits could show indistinguishable restriction patterns with REAmt, but could be discriminated using other typing methods such as RAPD-PCR, which although showing low reproducibility could be used as complementary to REAmt. Our results demonstrate that in spite of using autochthonous selected strains, the inoculation of musts with a particular strain do not guarantee its predominance and driving fermentation features. Of all yeast strains studied, strain no. 2 showed the best results in sensory testing and at the implantation process. Therefore, it could be used with commercial purposes for the production of Chacolí de Vizcaya/Bizkaiko Txakolina, especially when using musts from Folle Blanche(AU)


Assuntos
Microbiologia Industrial/métodos , Reação em Cadeia da Polimerase , Técnica de Amplificação ao Acaso de DNA Polimórfico , Saccharomyces/isolamento & purificação , Vinho/microbiologia , Saccharomyces/genética , Proteínas de Saccharomyces cerevisiae/biossíntese , DNA Fúngico/análise , DNA Mitocondrial/análise , Eletroforese em Gel de Campo Pulsado , Fermentação , Sulfeto de Hidrogênio/metabolismo , Fenótipo , Polimorfismo de Fragmento de Restrição , Proteínas/metabolismo , Saccharomyces/classificação , Saccharomyces/crescimento & desenvolvimento
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