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1.
J. physiol. biochem ; 74(2): 301-312, mayo 2018. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-178986

RESUMO

Apocynum venetum L., belonging to the family Apocynaceae, is a popular medicinal plant, which is commonly used in the treatment of hypertension, neurasthenia, and hepatitis in China. In the present study, the total flavonoids (TFs) were prepared from the leaves of A. venetum, and its protective effects on carbon tetrachloride (CCl4)-induced hepatotoxicity in a cultured HepG2 cell line and in mice were investigated. Cell exposed to 0.4% CCl4 (v/v) for 6 h led to a significant decrease in cell viability, increased LDH leakage, and intracellular reactive oxygen species (ROS). CCl4 also induced cell marked apoptosis, which was accompanied by the loss of mitochondrial membrane potential (MMP). Pretreatment with TFs at concentrations of 25, 50, and 100 μg/mL effectively relieved CCl4-induced cellular damage in a dose-dependent manner. In vivo, TFs (100, 200, and 400 mg/kg BW) were administered via gavage daily for 14 days before CCl4 treatment. The high serum ALT and AST levels induced by CCl4 were dose-dependently suppressed by pretreatment of TFs (200 and 400 mg/kg BW). Histological analysis also supported the results obtained from serum assays. Furthermore, TFs could prevent CCl4-caused oxidative damage by decreasing the MDA formation and increasing antioxidant enzymes (CAT, SOD, GSH-Px) activities in liver tissues. In summary, both in vitro and in vivo data suggest that TFs, prepared from A. venetum, showed a remarkable hepatoprotective and antioxidant activity against CCl4-induced liver damage


No disponible


Assuntos
Humanos , Animais , Masculino , Camundongos , Apocynum/química , Tetracloreto de Carbono/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Flavonoides/farmacologia , Alanina Transaminase/sangue , Apoptose , Aspartato Aminotransferases/sangue , Catalase/metabolismo , Testes de Função Hepática , Superóxido Dismutase/metabolismo , Potencial da Membrana Mitocondrial
2.
Eur. j. anat ; 17(4): 220-229, oct. 2013. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-134667

RESUMO

Oxidative stress induced by free radicals is known to be a common cause of liver damage and hepatic fibrosis. Anise oil and its compounds have been identified to have antioxidant, anti-inflammatory and antifibrinogenic properties that may play a role in the management of hepatic disorders and promote liver regeneration. Thus, the purpose of the study was to evaluate the effects of anise oil on hepatotoxicity induced by carbon tetrachloride in adult male albino rats. Sixty male albino rats were divided into control group, CCL4-treated group that was injected with 1 mg /kg CCL4 intraperitoneally (ip), CCL4+anise oil-treated group that was injected with 1 mg /kg of CCL4 and 0.5 ml/ kg of anise oil (ip), and anise oil-treated group that was injected with 0.5 ml/kg of anise oil. Animals received treatment for 4 weeks and sacrificed 24 hours after the last administration. Livers were removed and processed for light and electron microscopy analysis. The CCL4-treated group revealed loss of normal architecture of hepatic lobules, steatosis, necrosis, cholestasis, portal congestion and progressed grading of lobular inflammation, ballooning degeneration and fibrosis. On the other hand, the CCL4 + anise group showed reduced liver damage and increased signs of regeneration. We conclude that anise oil has a protective effect on liver damage caused by CCL4and promotes liver regeneration (AU)


No disponible


Assuntos
Animais , Masculino , Feminino , Ratos , Tetracloreto de Carbono/efeitos adversos , Tetracloreto de Carbono/uso terapêutico , Intoxicação por Tetracloreto de Carbono/complicações , Intoxicação por Tetracloreto de Carbono/veterinária , Estresse Oxidativo , Pimpinella/efeitos adversos , Pimpinella/toxicidade , Microanálise por Sonda Eletrônica/métodos , Microanálise por Sonda Eletrônica/veterinária , Fotomicrografia/métodos
3.
J. physiol. biochem ; 69(3): 359-370, sept. 2013.
Artigo em Inglês | IBECS | ID: ibc-121656

RESUMO

The purpose of this study was to evaluate the effect of pomegranate (Punica granatum) in inhibiting and reversing the nephrotoxicity of carbon tetrachloride, a potent oxidative stress inducer which induces cellular kidney damage. Rats were intraperitoneally injected with carbon tetrachloride (2 mL/kg body weight) which produced severe renal tissue damage, as demonstrated by decreased uric acid and dramatic elevation of urea and creatinine. In addition, carbon tetrachloride injection caused oxidative stress in rats, as evidenced by increased lipid peroxidation and nitrite/nitrate (NOx) concentrations in the renal tissue, along with a remarkable reduction in superoxide dismutase, catalase, glutathione transferase, glutathione reductase, glutathione peroxidase activities and glutathione content. We suggested that pomegranate juice was able to elevate the antioxidant defense system, clean up free radicals, lessen oxidative damages and protect the kidney against carbon tetrachloride-induced toxicity, thus having a potential protective effect (AU)


Assuntos
Animais , Ratos , 26622/uso terapêutico , Lesão Renal Aguda/tratamento farmacológico , Modelos Animais de Doenças , Substâncias Protetoras/farmacocinética , Tetracloreto de Carbono
4.
J. physiol. biochem ; 68(1): 29-35, mar. 2012.
Artigo em Inglês | IBECS | ID: ibc-122375

RESUMO

No disponible


Liver fibrosis is a major health problem that can lead to the development of liver cirrhosis and hepatocellular carcinoma. On the other hand, several antioxidants have been shown to possess protective effect against liver fibrosis. Therefore, in the present work, the effectiveness of curcumin, á-lipoic acid, and N-acetylcysteine in protecting against carbon tetrachloride (CCl4)-induced liver fibrosis as well as the mechanism(s) implicated in this protective effect was studied. The antioxidants used in this study resulted in hepatoprotective effect as evident by substantial (..) (AU)


Assuntos
Animais , Ratos , Curcumina/farmacocinética , Cirrose Hepática/tratamento farmacológico , Tetracloreto de Carbono/farmacocinética , Substâncias Protetoras/farmacocinética , Modelos Animais de Doenças , Ácido Tióctico/farmacocinética , Acetilcisteína/farmacocinética , Elementos de Resposta Antioxidante
5.
J. physiol. biochem ; 67(4): 519-530, dic. 2011.
Artigo em Inglês | IBECS | ID: ibc-122389

RESUMO

No disponible


Acetyl-L-carnitine (ALCAR) has been shown to prevent experimental selenite cataractogenesis, a manifestation of oxidative stress, but little is known about its potential in other settings of oxidative stress. The present study was based on the hypothesis that ALCAR prevents carbon tetrachloride (CCl4)-induced oxidative stress in vital tissues. Male albino Wistar rats were divided into three groups, each of six rats. Group I (control) rats received only vehicle (1 ml/kg b.w.) for 4 days; Group II (CCl4-exposed, untreated) rats received CCl4 (2 ml/kg b.w.) on the second and third days and vehicle on the first and fourth days; Group III (CCl4-exposed, ALCAR-treated) rats received ALCAR (200 mg/kg b.w.) for 4 days and CCl4 on the second and third days. All administrations were made intraperitoneally. After the experimental period, significantly (P < 0.05) elevated mean serum levels of aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate dehydrogenase were observed in Group II rats when compared to Group I and Group III rats. The mean levels of vitamin C, vitamin E, and reduced glutathione and the mean activities of superoxide dismutase, catalase, and glutathione peroxidase were significantly (P < 0.05) lower in samples of hemolysate and of liver, kidney, and brain tissues of Group II rats than those in Group I and Group III rats. The mean level of lipid peroxidation was significantly (P < 0.05) higher in Group II rats than that in Group I and Group III rats. Moreover, the CCl4-induced upregulation of inducible nitric oxide synthase expression was prevented by ALCAR in the liver and brain tissues. These results suggest that ALCAR is able to prevent the CCl4-induced oxidative stress (AU)


Assuntos
Animais , Ratos , Estresse Oxidativo , Acetilcarnitina/farmacocinética , Substâncias Protetoras/farmacocinética , Modelos Animais de Doenças , Tetracloreto de Carbono/farmacocinética , Estudos de Casos e Controles
6.
J. physiol. biochem ; 67(4): 569-576, dic. 2011. ilus
Artigo em Inglês | IBECS | ID: ibc-122394

RESUMO

No disponible


Coumarins are a vast group of natural compounds and some of them possess antioxidant activities. The comparison of the antioxidant activity of some coumarins with various chemical molecular structure has not been investigated in previous studies. Therefore, this study was aimed to investigate the hepatoprotective effect against carbon tetrachloride (CCl4) -induced hepatic injury by coumarin (1,2-benzopyrone) and coumarin derivatives, esculetin (6,7-dihydroxycoumarin), scoparone (6,7-dimethoxycoumarin), and 4-methylumbelliferone (7-hyroxy-4-methyl) in male Sprague–Dawley rats. Product of lipid peroxidation, malondialdehyde (MDA), activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) were evaluated for oxidative stress in hepatic injury. Gamma glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH) were detected in plasma as a biomarker of hepatic injury. Significantly elevated levels of MDA and lowered levels of SOD and CAT activities were observed in liver of rats exposed to CCl4, when compared to control values. Similarly, administration of CCl4 increased LDH and GGT levels in serum. Pre-treatment of rats with esculetin (35 mg kg−1, orally) and scoparone (35 mg kg−1, orally) significantly prevented CCl4-induced decrease in MDA levels and increase in SOD and CAT, whereas 4-methylumbelliferone (35 mg kg−1) and coumarin (30 mg kg−1) had no effect against CCl4-induced rise in serum enzymes. Esculetin and scoparone also showed protective properties as was evidenced in reduced LDH and GGT levels in serum. The results of this study indicate that the chemical structures of coumarins play an important role in the prevention of oxidative stress (AU)


Assuntos
Animais , Ratos , Cumarínicos/farmacocinética , Doença Hepática Crônica Induzida por Substâncias e Drogas/fisiopatologia , Estresse Oxidativo , Substâncias Protetoras/farmacocinética , Modelos Animais de Doenças , Tetracloreto de Carbono/farmacocinética
8.
J. physiol. biochem ; 65(3): 225-233, sept. 2009.
Artigo em Inglês | IBECS | ID: ibc-122867

RESUMO

No disponible


This study aimed to investigate whether treatments with vitamin E, L-carnitine and melatonin can protect against CCl4 and diabetes-induced hepatic oxidative stress. Hepatic oxidative stress was performed in rats through 50% v/v carbon tetrachloride (CCl4) (1 ml/kg/3days, i.p.), and through diabetes mellitus induced by streptozotocin (STZ) (40 mg/kg, i.p.). Vitamin E (100 mg/kg/day, i.p), L-carnitine (300 mg/kg/day, i.p.) and melatonin (10 mg/kg/day, i.p.) were injected for a period of 6 weeks. Thereafter, changes in serum glucose level, liver function tests, hepatic malondialdehyde (MDA) content, hepatic reduced glutathione (GSH) content, hepatic superoxide (..) (AU)


Assuntos
Animais , Ratos , Vitamina E/farmacocinética , Melatonina/farmacocinética , Carnitina/farmacocinética , Estresse Oxidativo , Diabetes Mellitus/fisiopatologia , Tetracloreto de Carbono/farmacocinética , Modelos Animais de Doenças , Substâncias Protetoras/farmacocinética , Antioxidantes/farmacocinética
9.
Ars pharm ; 48(4): 351-360, 2007. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-64398

RESUMO

Se examinó el extracto acuoso y etanólico (100 mg/kg) de Luffa acutangula Linn (frutos) para determinar la actividadantihepatotóxica en ratas druckrey mediante la hepatotoxicidad inducida por tetracloruro de carbono (CCl4)y paracetamol (PCM). Se demostró que el extracto posee un efecto hepatoprotector signifi cativo, ya que reduce losniveles séricos de transaminasas (SGPT y SGOT), fosfatasa alcalina (ALP) y bilirrubina. La signifi cativa actividadhepatoprotectora de Luffa acutangula es comparable a la de la silimarina, agente hepatoprotector estándar, lo quejustifi ca su uso en afecciones del hígado


The ethanolic and aqueous extract (100mg/kg) of Luffa acutangula Linn(fruits) was examined for antihepatotoxic activityin druckrey rats by inducing hepatotoxicity with Carbon tetrachloride(CCl4) and Paracetamol(PCM). The extracthas shown to posses signifi cant hepatoprotective effect by lowering the serum level of transaminases (SGPT & SGOT),Alkaline phosphatase (ALP) and bilirubin. The signifi cant hepatoprotective activity of Luffa acutangula is comparableto that standard hepatoprotective agent silymarin, which justify its use in liver affection


Assuntos
Ratos , Masculino , Feminino , Animais , Extratos Vegetais/química , Luffa acutangula/análise , Luffa acutangula/farmacologia , Luffa acutangula/uso terapêutico , Tetracloreto de Carbono/química , Tetracloreto de Carbono/síntese química , Acetaminofen/química , Acetaminofen/farmacologia , Silimarina/farmacologia , Fitoterapia/classificação , Fitoterapia , Luffa acutangula/provisão & distribução , Tetracloreto de Carbono/análise , Tetracloreto de Carbono/farmacologia , Tetracloreto de Carbono/farmacocinética , Silimarina/farmacocinética
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