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1.
Nutr. hosp ; 37(1): 46-55, ene.-feb. 2020. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-187573

RESUMO

Introducción: las enfermedades crónicas no transmisibles (ECNT) son la principal causa de muerte en todo el mundo. Los metabolitos secundarios provenientes de fuentes vegetales como Cnidoscolus aconitifolius pueden usarse como coadyuvante en la prevención de las enfermedades relacionadas con el estrés oxidativo y la inflamación, tales como las ECNT. Objetivo: se evaluó la actividad antioxidante y antiinflamatoria in vitro de los compuestos biológicamente activos de extractos de C. aconitifolius. Métodos: se determinó el contenido de fenoles, flavonoides, flavanonas e hidroflavonoles. El potencial antioxidante se determinó con los ensayos de 1,1-difenil-2-picrilhidrazilo (DPPH), ácido 2,2'-azinobis (3-etilbenzotiazolina-6-sulfónico) (ABTS) y la actividad inhibitoria de la enzima convertidora de la angiotensina (ACE). Para la actividad antiinflamatoria se utilizaron la reacción en cadena de la polimerasa (PCR) cuantitativa en tiempo real y el ensayo por inmunoabsorción ligado a enzimas (ELISA) en macrófagos diferenciados de monocitos THP-1 y estimulados con lipopolisacárido (LPS). Resultados: el extracto acuoso registró el mayor contenido de fenoles (70,61  0,07 g/100 g de extracto) y el extracto etanólico registró el mayor contenido de flavonoides (47,76  4,84 g/100 g de extracto), flavanonas y dihidroflavonoles (70,10  7,29 g/100 g de extracto). El extracto acetónico registró la mayor inhibición del radical DPPH (49,85 ± 5,30 %) mientras que el etanólico presentó la mayor inhibición del radical ABTS (41,01 ± 3,81 %). Los extractos etanólico y acuoso inhibieron la ECA. El extracto etanólico tuvo la mayor actividad antiinflamatoria al reducir la expresión génica de TNF- en un 39,78 % y la de IL-6 en un 97,81 %, y su producción en un 46 % y un 48,38 %, respectivamente. Conclusiones: los extractos mostraron in vitro su potencial antioxidante y antiinflamatorio por su contenido en compuestos bioactivos


Introduction: noncommunicable diseases (NCDs) are the main cause of death worldwide. Secondary metabolites from plant sources such as Cnidoscolus aconitifolius may be used as adjuvants in the prevention of diseases related to oxidative stress and inflammation such as NCDs. Objective: the in vitro antioxidant and anti-inflammatory activities associated with biologically active compounds in C. aconitifolius extracts were evaluated. Methods: the contents of phenols, flavonoids, flavonones and hydroflavonoles were determined. The potential antioxidant activity was determined with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, and angiotensin-converting enzyme (ACE) activity. For anti-inflammatory activity quantitative PCR and enzyme-linked immunosorbent assay (ELISA) tests were used in macrophages derived from THP-1 monocytes and stimulated with LPS. Results: the aqueous extract recorded the highest phenolic content (70.61  0.07 g/100 g of extract), and the ethanolic extract registered the highest content in flavonoids (47.76  4.84 g/100 g of extract), flavonones and dihydroflavonoles (70.10  7.29 g/100 g of extract). The acetone extract obtained the highest DPPH inhibition (49.85 ± 5.30 %), while the ethanolic extract showed the highest ABTS inhibition (41.01 ± 3.81 %). The etanolic and aqueous extracts had the highest ACE inhibition. The ethanolic extract had the highest anti-inflammatory activity, decreasing gene expression for TNF- by 39.78 % and for IL-6 by 97.81 %, and their production by 46 % and 48.38 %, respectively, in macrophages stimulated with LPS. Conclusions: these extracts demonstrated in vitro their antioxidant and anti-inflammatory potential due to their content of bioactive compounds


Assuntos
Técnicas In Vitro , Antioxidantes/isolamento & purificação , Jatropha/metabolismo , Inflamação/tratamento farmacológico , Extratos Vegetais/farmacologia , Antioxidantes/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Inflamação/metabolismo , Doença Crônica/prevenção & controle , Fenóis , Flavonoides , Flavanonas , Ensaio de Imunoadsorção Enzimática
2.
Nutr. hosp ; 36(2): 470-478, mar.-abr. 2019. tab, graf, ilus
Artigo em Espanhol | IBECS | ID: ibc-184344

RESUMO

Introducción: en la actualidad, la población adulta mayor se encuentra en aumento y, con ello, la presencia de enfermedades. Los compuestos fenólicos son sustancias que tienen propiedades antioxidantes y pueden impactar en la prevención del daño oxidativo, muy relacionado con el inicio de diversas enfermedades. La mayoría de los compuestos fenólicos son bioaccesibles y biodisponibles mediante vías mecánicas, enzimáticas y químicas. Sin embargo, durante el envejecimiento se modifican estos factores afectando la absorción de estos compuestos. Objetivo: conocer la información reciente relacionada con el consumo de compuestos fenólicos y sus implicaciones para la salud en los adultos mayores. Métodos: se realizó una búsqueda en diferentes bases académicas y/o motores de búsqueda (EBSCOHOST, PubMed y Science Direct) sobre consumo total de fenoles en adultos mayores. Esta información fue analizada y subclasificada considerando enfermedades crónicas. Resultados: los adultos mayores tienen una ingesta de compuestos fenólicos que oscila de 280 ± 130 a 2.771 ± 1.552 mg/día y la cantidad que refleja un beneficio frente enfermedades cardiovasculares, síndrome metabólico y diabetes en adultos mayores oscila de 322 ± 153 a 2.861 mg/día. Conclusión: en algunos casos, el consumo de polifenoles en el adulto mayor no es suficiente para generar una prevención frente a diferentes enfermedades, por lo que se recomienda incrementar su cantidad. Existen diferentes situaciones que modifican la bioaccesibilidad y biodisponibilidad, entre ellas la deficiencia de transportadores de polifenoles, por lo que la cantidad necesaria se modifica para llevar a cabo su acción en el organismo. Además, existen pocos estudios de este tipo en esta población, por lo que se recomienda realizar más investigaciones


Introduction: currently, the elderly population is increasing and with it, the presence of diseases. Phenolic compounds are substances that have antioxidant properties which can impact on the prevention of oxidative damage, closely related to the onset of various diseases. Most phenolic compounds are bioaccessible and bioavailable through mechanical, enzymatic and chemical routes. However, during aging these factors are modified, affecting the absorption of these compounds. Objective: to know the recent information related to the consumption of phenolic compounds and implications for health in the elderly. Methods: a search was made in different academic bases and/or search engines (EBSCOHOST, PubMed and Science Direct) about total phenol consumption in older adults. This information was analyzed and subclassified considering chronic diseases. Results: older adults have an intake of phenolic compounds ranging from 280 ± 130 to 2,771 ± 1,552 mg/day and the amount that reflects a benefit against cardiovascular diseases, metabolic syndrome and diabetes in older adults ranges from 322 ± 153 to 2,861 mg/day. Conclusion: in some cases, the consumption of polyphenols in the elderly is not enough to generate a prevention against different diseases, so increasing their quantity is recommended. There are different situations that modify bioaccessibility and bioavailability, including the deficiency of polyphenol transporters, so the amount needed is modified to carry out its action in the body. There are few studies of this type in this population, so more research is recommended


Assuntos
Humanos , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Antioxidantes , Dieta , Polifenóis , Doenças Cardiovasculares/prevenção & controle , Flavonoides
3.
Ars pharm ; 59(2): 61-67, abr.-jun. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-177721

RESUMO

Objective: Glycyrrhizic acid is a widely used medicinal component as an anti-inflammatory agent, anti ulcer agent, anti-allergy agent and anti-psoriatic agent. The present investigation deals with the extraction of glycyrrhizic acid from licorice roots and evaluating its in-vitro anti inflammatory activity. Methods: Glycyrrhizic acid was extracted using the procedure of maceration. The extract was evaluated for its physicochemical property, biochemical tests and phytochemical properties. The Ii vitro anti- inflammatory activity was evaluated by albumin denaturation technique Results: The results showed that the ash value and the extractive values for the extract were found to be in the limit as given by Ayurvedic Pharmacopoeia of India. Presence of flavonoids, saponins and triterpinoids were identified in the extract from phytochemical parameters. Thin layer chromatographic technique showed a retention value of 0.5 cm. The percentage inhibition showed that the extract is having some potential of healing inflammation. Conclusion: Glycyrrhizic acid was successfully extracted from licorice roots. The evaluation parameters showed the presence of less impurity in the extract, with the potential of having anti-inflammatory property


Objetivo: El ácido glicirrícico es un componente medicinal ampliamente utilizado como agente antiinflamatorio, agente antiulceroso, agente antialérgico y agente anti-psoriásico. La presente investigación trata de la extracción de ácido glicirrícico a partir de raíces de regaliz y la evaluación de su actividad antiinflamatoria in vitro. Métodos: el ácido glicirrícico fue extraído usando el procedimiento de la maceración. El extracto fue evaluado por su propiedad fisicoquímica, pruebas bioquímicas y propiedades fitoquímicas. La actividad antiinflamatoria in vitro fue evaluada por la técnica de desnaturalización de albúmina Resultados: los resultados demostraron que el valor de la ceniza y los valores extractivos para el extracto se encontraron en el valor límite según lo dado por la farmacopea de Ayurveda de la India. La presencia de flavonoides, de saponinas y de triterpenoides fue identificada en el extracto mediante parámetros fitoquímico. La técnica cromatográfica en capa delgada demostró un valor de retención de 0,5 centímetros. La inhibición porcentual mostró que el extracto tiene algún potencial de curación de la inflamación. Conclusión: el ácido glicirrícico fue extraído con éxito de las raíces de regaliz. Los parámetros de evaluación mostraron la presencia de menos impureza en el extracto, con el potencial de tener propiedades antiinflamatorias


Assuntos
Ácido Glicirrízico/farmacologia , Anti-Inflamatórios/farmacologia , Glycyrrhiza uralensis/química , Saponinas/farmacologia , Flavonoides/farmacologia , Técnicas In Vitro , Absorção Fisico-Química , Cromatografia , Saponinas/isolamento & purificação , Flavonoides/isolamento & purificação , Inflamação/tratamento farmacológico , Mediadores da Inflamação/metabolismo , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia
4.
J. physiol. biochem ; 74(2): 301-312, mayo 2018. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-178986

RESUMO

Apocynum venetum L., belonging to the family Apocynaceae, is a popular medicinal plant, which is commonly used in the treatment of hypertension, neurasthenia, and hepatitis in China. In the present study, the total flavonoids (TFs) were prepared from the leaves of A. venetum, and its protective effects on carbon tetrachloride (CCl4)-induced hepatotoxicity in a cultured HepG2 cell line and in mice were investigated. Cell exposed to 0.4% CCl4 (v/v) for 6 h led to a significant decrease in cell viability, increased LDH leakage, and intracellular reactive oxygen species (ROS). CCl4 also induced cell marked apoptosis, which was accompanied by the loss of mitochondrial membrane potential (MMP). Pretreatment with TFs at concentrations of 25, 50, and 100 μg/mL effectively relieved CCl4-induced cellular damage in a dose-dependent manner. In vivo, TFs (100, 200, and 400 mg/kg BW) were administered via gavage daily for 14 days before CCl4 treatment. The high serum ALT and AST levels induced by CCl4 were dose-dependently suppressed by pretreatment of TFs (200 and 400 mg/kg BW). Histological analysis also supported the results obtained from serum assays. Furthermore, TFs could prevent CCl4-caused oxidative damage by decreasing the MDA formation and increasing antioxidant enzymes (CAT, SOD, GSH-Px) activities in liver tissues. In summary, both in vitro and in vivo data suggest that TFs, prepared from A. venetum, showed a remarkable hepatoprotective and antioxidant activity against CCl4-induced liver damage


No disponible


Assuntos
Humanos , Animais , Masculino , Camundongos , Apocynum/química , Tetracloreto de Carbono/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Flavonoides/farmacologia , Alanina Transaminase/sangue , Apoptose , Aspartato Aminotransferases/sangue , Catalase/metabolismo , Testes de Função Hepática , Superóxido Dismutase/metabolismo , Potencial da Membrana Mitocondrial
5.
Rev. esp. nutr. comunitaria ; 24(1): 0-0, ene.-mar. 2018. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-178344

RESUMO

Fundamentos: Una dieta basada en alimentos y bebidas con alto contenido en azúcar y almidón digestible se considera un factor de riesgo de padecer enfermedades crónicas no trasmisibles tales como obesidad y diabetes tipo 2. El objetivo es resumir las evidencias disponibles sobre la efectividad de los compuestos fenólicos en el metabolismo de carbohidratos y en la reducción del riesgo y el tratamiento de estas patologías. Métodos: Revisión bibliográfica mediante los sistemas de búsqueda PubMed y WOS para la identificación de compuestos fenólicos con efecto en el metabolismo de carbohidratos y las patologías metabólicas relacionadas. Resultados: Los compuestos fenólicos actúan a diferentes niveles: vía formación de complejos compuesto fenólico-carbohidrato, vía inhibición enzimática, modulación del transporte y regulación de la liberación de hormonas relacionadas con el metabolismo de los carbohidratos. Su efectividad puede ser igual o superior a la de fármacos utilizados comúnmente en el tratamiento de la diabetes, sin los efectos secundarios que se asocian a estos últimos. Conclusiones: Los flavonoides son los compuestos fenólicos de la dieta que podrían ser más efectivos en la regulación del metabolismo de carbohidratos. Estos pueden inhibir enzimas, modular transportadores; así como, estimular la secreción de hormonas de saciedad


Background: A diet based on foods and beverages with high sugar content and digestible starch is considered a risk factor of non-communicable chronic diseases such as obesity and type 2 diabetes. The aim is to summarize the evidence available on the effect of phenolic compounds in the metabolism of carbohydrates and in the reduction of risk and treatment of these pathologies. Methods: Literature search using PubMed and WOS as search systems for the identification of phenolic compounds with an effect on carbohydrate metabolism and related metabolic pathologies. Results: Phenolic compounds act at different levels: via complex formation of phenolic-carbohydrate compounds, via enzymatic inhibition, modulation of transporters and regulation of the release of hormones related to carbohydrate metabolism. Their effectiveness may be equal or superior to that of drugs commonly used in the treatment of diabetes, but without the side effects that are associated with the latter. Conclusions: Flavonoids are the phenolic compounds found in diet that are effective in regulating carbohydrate metabolism. They can inhibit enzymes, modulate transporters; as well as, stimulate the secretion of satiety hormones


Assuntos
Humanos , Compostos Fenólicos/análise , Metabolismo dos Carboidratos/fisiologia , Flavonoides/análise , Diabetes Mellitus Tipo 2/prevenção & controle , Obesidade/prevenção & controle , Substâncias Protetoras/análise , Resposta de Saciedade/fisiologia
6.
Neurología (Barc., Ed. impr.) ; 32(8): 516-522, oct. 2017. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-166982

RESUMO

Introducción: Se evaluó el efecto protector de 2 presentaciones comerciales de Ginkgo biloba sobre las alteraciones motoras inducidas por el consumo de jugo de yuca (Manihot esculenta Crantz) en ratas macho Wistar. Método: Los efectos se evaluaron en las pruebas de campo abierto y nado a los 0, 7, 14, 21 y 28 días de tratamiento, una hora después de la administración correspondiente. Resultados: A partir del día 21 del consumo de jugo de yuca incrementó el número de cuadros cruzados en campo abierto y, en la prueba de nado, produjo el nado lateral a partir del día 7, con respecto al grupo control. Conclusión: Los extractos de Ginkgo biloba previnieron las alteraciones motoras asociadas al consumo de jugo de yuca, probablemente por el contenido de flavonoides presentes en ambas presentaciones de Ginkgo biloba (AU)


Introduction: This study evaluated the protective effects of 2 commercial formats of Ginkgo biloba on motor alterations induced by cassava (Manihot esculenta Crantz) juice consumption in male Wistar rats. Methods: The effects were evaluated with the open field and swim tests at 0, 7, 14, 21, and 28 days of treatment, one hour after administering the product. Results: Compared to controls, open field crossings increased after day 21 of cassava juice consumption, and lateral swimming in the swim test was reported after day 7. Conclusion: Ginkgo biloba extracts prevented motor alterations associated with cassava juice consumption, probably due to the flavonoid content in both formats of Ginkgo biloba (AU)


Assuntos
Animais , Ratos , Ginkgo biloba , Preparações de Plantas/farmacocinética , Transtornos das Habilidades Motoras/induzido quimicamente , Yucca , Substâncias Protetoras/farmacocinética , Transtornos das Habilidades Motoras/prevenção & controle , Natação , Flavonoides/isolamento & purificação , Espectrofotometria
7.
Nutr. hosp ; 34(4): 841-846, jul.-ago. 2017. tab
Artigo em Espanhol | IBECS | ID: ibc-165345

RESUMO

Antecedentes: estudios previos han encontrado que el consumo de flavonoides se asocia a menor riesgo de deterioro cognitivo. Sin embargo, el efecto del consumo habitual de chocolate sobre el estado cognitivo no está bien esclarecido. Objetivo: examinar si el consumo habitual de chocolate se asocia con un mejor estado cognitivo en adultos mayores españoles. Metodología: estudio transversal, utilizando datos del estudio ENRICA-Senior. El consumo habitual de chocolate fue evaluado con una historia dietética informatizada. Los participantes fueron divididos en las siguientes categorías, de acuerdo a su consumo de chocolate: sin consumo, < 10 g/d y ≥ 10 g/d. El estado cognitivo fue determinado mediante la versión del test Mini-Mental State Examination (MMSE) para España. Los puntos de corte para definir deterioro cognitivo leve (DCL) fueron: ≤ 25, ≤ 24 y ≤ 23. Se utilizaron modelos de regresión lineal y logística ajustados por los principales factores confusores para calcular coeficientes beta y odds ratios (OR) de asociación. Resultados: en comparación con el grupo de personas que no reportaron consumir chocolate, aquellos con un consumo habitual de ≥ 10 g/d obtuvieron una mayor puntuación en el MMSE (coeficiente beta e intervalo de confianza al 95% [IC 95%] del modelo ajustado: 0,26 [0,02- 0,50]; p de tendencia lineal = 0,05). Cuando examinamos la asociación solo con chocolate negro, los resultados también fueron significativos (coeficiente beta: 0,48 [IC 95%: 0,18-0,78]; p de tendencia lineal < 0,001). El consumo de chocolate total no se asoció con DCL en ninguna de las definiciones propuestas. Sin embargo, el consumo de chocolate negro se asoció con menor probabilidad de DCL (OR e IC 95% para MMSE ≤ 25: 0,39 [0,20-0,77]; para MMSE ≤ 24: 0,26 [0,10-0,67]; y para MMSE ≤ 23: 0,25 [0,07-0,82]). Conclusión: estos resultados sugieren que puede existir una asociación entre consumo de chocolate y mejor función cognitiva en personas mayores (AU)


Background: There are associations described between dementia, mild cognitive impairment (MCI) and foods with a high content of polyphenols. Objective: To assess the influence of habitual chocolate consumption over the MMSE in Spanish older adults. Methodology: Cross-sectional study, using data of the follow-up of the Seniors-Study on Nutrition and Cardiovascular Risk in Spain (ENRICA) cohort. Habitual chocolate consumption in the last year was assessed with a computerized dietary history; differences between dark chocolate and milk chocolate were recorded. Chocolate intake was classified into the following categories: no consumption, < 10 g/day, and ≥ 10 g/day. Validated MMSE scores for Spain were obtained during an interview and different cutoff points were used to define ≤ 25, ≤ 24 and ≤ 23. Linear and logistic regression models were used to calculate adjusted beta coefficients and odds ratios (OR). Results: Compared to non-consumers, participants with a habitual chocolate consumption of ≥ 10 g/d had a better MMSE score (adjusted beta coefficient and 95% confidence interval: 0.26 (0.02-0.50; p trend = 0.05); for dark chocolate, the results were also statistically significant (0.48 [0.18-0.78]; p trend < 0.001). Total chocolate consumption was not associated with higher likelihood of having MCI. However, dark chocolate consumption was associated with less likelihood of MCI (OR and 95%CI for MMSE ≤ 25: 0.39 [0.20-0.77]; for MMSE ≤ 24: 0.26 [0.10-0.67]; and for MMSE ≤ 23: 0.25 [0.07-0.82]). Conclusion: Our results suggest that habitual dark chocolate consumption might improve cognitive function among the older population (AU)


Assuntos
Humanos , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Saúde do Idoso , Comportamento Alimentar/fisiologia , Cacau , Flavonoides , Transtornos Cognitivos/prevenção & controle , Estudos Transversais/métodos , Razão de Chances , Modelos Lineares , Modelos Logísticos , Estudos de Coortes , Intervalos de Confiança
8.
An. R. Acad. Farm ; 82(3): 297-302, jul.-sept. 2016.
Artigo em Inglês | IBECS | ID: ibc-158072

RESUMO

Bioactive compounds isolated from plants have gained a lot of attention in recent years. Among them flavonoids, which consist of a large group of polyphenolic compounds, are at the forefront in the treatment of various diseases including cancer. Flavonoids possess anti-cancer properties and they exert their curative effect by modulating different cellsignalling pathways like the Nf-kB pathway, PI3K/AKT/mTOR pathway and the JAK/STAT pathway. Flavonoids also possess anti-oxidant activity and they regulate the redox status and prevent damage caused by oxidative stress. Chemokines and cytokines play a key role in mediating the inflammatory response in a cell. Consequently, more inflammatory markers are recruited to the site of inflammation that leads to increased ROS and cause damage at the site of accumulation. The present review covers the recent studies, in vitro and in vivo, that highlight the promising potential of flavonoids in treating cáncer


Los compuestos bioactivos aislados de las plantas han ganado mucha atención en los últimos años. Entre ellos los flavonoides, que consisten en un gran grupo de compuestos polifenólicos, están en la vanguardia del tratamiento de diversas enfermedades incluyendo el cáncer. Los flavonoides poseen propiedades anticancerígenas y ejercen su efecto curativo mediante la modulación de diferentes vías de señalización intracelular como la vía Nf-kB, PI3K / AKT / mTOR y la vía JAK / STAT. Los flavonoides también poseen actividad antioxidante regulando el estado redox y previniendo los daños causados por el estrés oxidativo. Las quimiocinas y citocinas juegan un papel clave en la mediación de la respuesta inflamatoria en las células. Por lo tanto, el aumento de los marcadores inflamatorios que son reclutados en el sitio de inflamación conduce a un aumento de las especies reactivas del oxígeno causando daños en el lugar de su acumulación. La presente revisión abarca los estudios más recientes, tanto in vitro como in vivo, en donde se destaca el potencial que presentan los flavonoides en el tratamiento del cáncer


Assuntos
Humanos , Flavonoides/farmacocinética , Neoplasias/prevenção & controle , Polifenóis/farmacocinética , Anticarcinógenos/farmacocinética , Substâncias Protetoras/farmacocinética , Transdução de Sinais
9.
J. physiol. biochem ; 72(2): 269-280, jun. 2016. graf
Artigo em Inglês | IBECS | ID: ibc-168271

RESUMO

Morin is a natural bioflavonoid that exhibits antioxidant and anti-inflammatory properties. The present study was designed to evaluate the effect of morin on insulin resistance, oxidative stress, and inflammation in a high-fat-diet (HFD)-induced obese mice. Obesity was induced in ICR mice by feeding a HFD (60 % kcal from fat) for 12 weeks. After the first 6 weeks, obese mice were treated with morin (50 or 100 mg/kg/day) once daily for further 6 weeks. Blood glucose, lipid profile, insulin, leptin, adiponectin, and markers of oxidative stress and inflammation were then measured. Liver was excised, subjected to histopathology, glycogen determination, and gene and protein expression analysis. Morin administration reduced blood glucose, serum insulin, leptin, malondialdehyde, interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) levels and increased serum adiponectin levels. Moreover, there was a reduction in serum lipid and liver triglyceride levels. Liver histology indicated that morin limited accumulation of lipid droplets. Interestingly, morin reduced expression of hepatic sterol regulatory element binding protein 1c (SREBP1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) and up-regulated hepatic carnitine palmitoyltransferase 1a (CPT1a) expression. Morin also stimulated glycogen storage and suppressed phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) protein expression. Furthermore, hepatic superoxide dismutase (SOD) and catalase (CAT) expression were increased after morin treatment. These findings indicate that morin has a positive effect in the HFD-induced obesity condition by suppressing lipogenesis, gluconeogenesis, inflammation, and oxidative stress activities (AU)


No disponible


Assuntos
Animais , Feminino , Fígado , Obesidade , Flavonoides/uso terapêutico , Antioxidantes/uso terapêutico , Resistência à Insulina , Anti-Inflamatórios não Esteroides/uso terapêutico , Hiperglicemia , Hiperlipidemias , Hiperinsulinismo , Dieta Hiperlipídica/efeitos adversos , Estresse Oxidativo , Lipogênese , Regulação da Expressão Gênica , Fármacos Antiobesidade/administração & dosagem , Relação Dose-Resposta a Droga
10.
Av. odontoestomatol ; 32(1): 35-44, ene.-feb. 2016.
Artigo em Espanhol | IBECS | ID: ibc-148177

RESUMO

Se realiza una síntesis de las características estructurales y moleculares de las membranas celulares humanas y de las paredes celulares de las plantas medicinales (CWP). El presente análisis soporta una hipótesis acerca de las relaciones existente entre ambas. El propósito es establecer el mecanismo de acción en el tratamiento local de irritaciones, quemaduras, abrasiones, pequeñas úlceras y reacciones agudas ampollares alérgicas, y enfermedad de las encías. Las proteínas de las paredes celulares de las plantas son proteínas extracelulares glicosiladas, polisacáridos, proteasas y lectinas. Acerca del 90% de las CWP son capaces de realizar funciones bioquímicas y biológicas. Su actividad antiinflamatoria ha sido investigada por varios autores como una inhibición del ácido araquidónico metabolizado por flavonoides. Investigaciones clínicas sugieren que las plantas medicinales aceleran la curación de las heridas ya que ellas aumentan la síntesis de colágeno y de proteoglicanos, promoviendo la reparación de los tejidos (AU)


Previously, a synthesis is presented about structural and molecular characteristics of human cell´s membranes and cell´s walls of medicinal plants. This analyses support an hypothesis about the relationships between both of them. The purpose is to establish the possibility of using it, as local treatment on irritations, burns, abrasions, small ulcers, acute bullous allergic reaction, gums illness and fungal infections of oral mucosa. Cell wall proteins (CWP) are glycosided proteins and polysaccharides, proteasas and lectins. They have been described as being extracellular. About 90% of CWP are capable to realize biochemical and biological functions. Anti-inflammatory activity and inhibition of arachidonic acid metabolism by flavonoids, isolated from medicinal plants have been studied. Clinical investigations suggest that medicinal plants accelerate wound healing because they increase collagen and proteoglycan syntesis promoting tissue repair (AU)


Assuntos
Humanos , Plantas Medicinais , Extratos Vegetais/farmacocinética , Doenças Estomatognáticas/tratamento farmacológico , Mucosa Bucal , Ácidos Eicosanoicos/farmacocinética , Flavonoides/farmacocinética
11.
Nutr. clín. diet. hosp ; 36(3): 170-180, 2016. ilus, tab
Artigo em Português | IBECS | ID: ibc-155470

RESUMO

Introdução: A obesidade, caracterizada pelo desequilíbrio do metabolismo energético, consequência de padrões alimentares com sobrecarga de nutrientes, desencadeia inflamação subclínica, por meio da secreção de mediadores inflamatórios como: IL-2, IL-4, IL-13, IL-15 e IFN-γ e estresse oxidativo que promovem alterações metabólicas que possivelmente modulam o metabolismo energético e seus componentes. Assim, a adoção de padrões alimentares saudáveis, com a inserção de frutas ricas em flavonoides, que possuem ação antioxidante e anti-inflamatória, tem efeito direto e benéfico na modulação do metabolismo energético, por meio da modulação da síntese de mediadores inflamatórios, que alteram parâmetros antropométricos, de composição corporal e bioquímicos e, assim, previnem a obesidade. Objetivos: Esse artigo tem por objetivo apresentar e discutir estudos de intervenção com frutas ricas em flavonoides que contribuíram para a modulação de mediadores antropométricos, de composição corporal, bioquímicos e inflamatórios que modularam o metabolismo energético. Métodos: Consiste em revisão sistemática no qual realizous-e uma sumarização de ensaios clínicos que consistiam no consumo de frutas ricas em flavonoides (‘berries’) em relação ao seu potencial efeito anti-inflamatório e seus efeitos sobre mediadores inflamatórios e do metabolismo energético. As investigações clínicas foram identificadas na base dados do PubMed, Scielo e Lilacs. Resultados: Dos estudos encontrados, a maioria apresentou efeito direto da intervenção com frutas ricas em flavonoides e a redução de mediadores inflamatórios. Além disso, observou-se que a intervenção com frutas modulou parâmetros bioquímicos, antropométricos e de composição corporal. Conclusão: Associação direta entre secreção de biomarcadores inflamatórios e alterações no metabolismo energético está bem elucidado na literatura. Assim como, há indicações de que a inserção de frutas ricas em flavonoides, além de melhorar o padrão alimentar e a composição corporal, reduz a secreção destes biomarcadores e, consequemente, modula o metabolismo energético, prevenindo a obesidade (AU)


Introduction: Obesity, characterized by an imbalance in energy metabolism as a consequence of dietary patterns nutrient loading, triggers subclinical inflammation through the secretion of inflammatory mediators such as IL-2, IL-4, IL-13, IL-15 and IFN-γ which promote oxidative stress and metabolic changes that possibly modulate energy metabolism and its components. Thus, the adoption of healthier eating patterns, with the inclusion of fruits rich in flavonoids, which have antioxidant and anti-inflammatory, have a direct and beneficial effect on the modulation of energy metabolism, modulating the inflammatory mediators synthesis that alter anthropometric, biochemical and body composition, consequently, preventing obesity. Objectives: This article aims to present and discuss intervention studies with fruits rich in flavonoids that contributed to the regulation of anthropometric, body composition, biochemical and inflammatory mediators modulating the energy metabolism. Methods: Consists of systematic review in which we carried out a summarization of clinical trials consisting in the consumption of fruits rich in flavonoids (‘berries’) in relation to its potential anti-inflammatory effect and their effects on inflammatory mediators and energy metabolism. Clinical investigations have been identified in the database PubMed, Lilacs and Scielo. Results: Of the found studies, most had direct effect of intervention with fruits rich in flavonoids and reduction of inflammatory mediators. Furthermore, it was observed that the fruit intervention modulated biochemical, anthropometric and body composition parameters. Conclusion: A direct association between secretion of inflammatory biomarkers and changes in energy metabolism is well elucidated in the literature. Just as there are indications that the inclusion of fruits rich in flavonoids, besides improve eating patterns and body composition, reduces the secretion of these biomarkers and consequently, modulates energy metabolism and prevents obesity (AU)


Assuntos
Humanos , Flavonoides/farmacocinética , Mediadores da Inflamação/análise , Obesidade/metabolismo , Metabolismo Energético , Frutas , Inflamação/tratamento farmacológico , Composição Corporal
12.
Arch. esp. urol. (Ed. impr.) ; 68(10): 739-749, dic. 2015. graf, ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-146544

RESUMO

Objetivo: Determinar el efecto preventivo sobre la litiasis renal de una formulación botánica formada por Herniaria glabra, Agropyron repens, Equisetum arvense y Sambucus nigra en un modelo experimental de nefrolitiasis en ratas. Métodos: Seis grupos de animales con seis ratas Wistar macho cada uno fueron inducidos a nefrolitiasis mediante el tratamiento con etilenglicol (EG) 0,75% y cloruro de amonio 1% durante tres días y posteriormente con EG durante 15 días más. Un grupo fue tratado con placebo (grupo control) y los otros grupos (grupos tratados) fueron tratados con 30 mg/Kg, 60 mg/Kg, 125 mg/Kg, 250 mg/kg y 500 mg/Kg de la formulación de extractos de plantas (FEP). Se midió el volumen de agua ingerida y de orina excretada durante 24 h en diferentes días del experimento y se determinó la diuresis, cristaluria y bioquímica. Se realizó el análisis histológico del riñón. La caracterización fitoquímica de la FEP se realizó mediante técnicas cromatográficas. Resultado: La cantidad de depósitos de cristales de oxalato de calcio (OxCa) de los animales tratados con 125 mg/Kg de la FEP y el número de microcalcificaciones en todos los grupos tratados con la FEP fue menor comparado con el grupo control, siendo las diferencias estadísticamente significativas (d. e. s.). La presencia de fibrosis subcapsular fue mayor en el grupo control que en los grupos tratados (d. e. s.). La diuresis de los grupos tratados con 125 mg/Kg y 500 mg/Kg de la FEP fue mayor que la del grupo control (d. e. s.). El análisis fitoquímico demostró la presencia de flavonoides, ácidos dicarboxílicos y saponinas. Conclusiones: La administración de la FEP previene la formación de cristales de OxCa y de microcalcificaciones en el riñón y disminuye el riesgo de fibrosis subcapsular renal. La dosis de 125 mg/Kg de la FEP es la que presenta un mayor efecto sobre los parámetros estudiados (AU)


Objective: To determine the effect of a botanical formulation of Herniaria glabra, Agropyron repens, Equisetum arvense, and Sambucus nigra as a preventive agent in an experimentally induced nefrolithiasis model in rats. Methods: Six groups of six Wistar male rats each were induced for nefrolithiasis by treatment with 0.75% ethylene glycol (EG) and 1% ammonium chloride for three days and then EG only for 15 days. One group was treated with placebo (control group) and the other groups (treated groups) were treated with 30 mg/Kg, 60 mg/Kg, 125 mg/Kg, 250 mg/Kg and 500 mg/Kg of the plant extract formulation (PEF). 24-h urine and water samples were collected one day before EG administration and at 7, 13 and 18 days to determine diuresis, crystalluria and urine biochemistry. The kidneys were removed for histological analysis. The phytochemical characterization of PEF and each of its component plant extracts was performed using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Results: Animals treated with 125 mg/Kg of the PEF had statistically significantly lower calcium oxalate crystals deposits content compared to the control group. All PEF doses statistically significantly decreased the number of microcalcifications compared to the control group. Furthermore, the number of kidneys affected by subcapsular fibrosis was statistically significantly higher in control group than in treated groups with the PEF. The diuresis of the 125 mg/Kg and 500 mg/Kg PEF-treated groups was statistically significantly higher than that of the control group. A phytochemical analysis demonstrated the presence of flavonoids, dicarboxylic acids and saponins. Conclusions: Treatment with PEF prevents deposits of calcium oxalate crystals formation and of microcalcifications in the kidney, and reduces the risk of fibrosis subcapsular. 125 mg/Kg of PEF is the dose that has a greater effect on the studied parameters (AU)


Assuntos
Animais , Feminino , Masculino , Ratos , Nefrolitíase/diagnóstico , Nefrolitíase/terapia , Nefrolitíase/veterinária , Agropyron , Equisetum arvense/uso terapêutico , Sambucus nigra , Diurese , Cálculos Renais/tratamento farmacológico , Etilenoglicol/uso terapêutico , Cloreto de Amônio/uso terapêutico , Modelos Animais , Rim/anatomia & histologia , Flavonoides/análise , Flavonoides/uso terapêutico , Oxalato de Cálcio/uso terapêutico , Ratos Wistar , Plantas/metabolismo , Análise de Variância , Análise Estatística
13.
Nutr. hosp ; 32(5): 1885-1893, nov. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-145513

RESUMO

Background: exercise-induced muscle damage (EIMD) is a multifactorial phenomenon that induces muscle function loss because of mechanical and immune stressor stimuli. This immunological stress is mostly caused by inflammation and increased oxidative status. Cherries are fruits that contain a phenolic compound known as anthocyanin, which serves as a pigment in natura. However, research suggests this pigment might provide a potent antioxidant and anti-inflammatory strategy when consumed by humans. Objectives: the aim of this study was to critically review the literature on cherry consumption focusing on identifying protective strategies against EIMD conferred by it. Methods: a research was performed in PubMed database. This review presents the results about cherry consumption and EIMD. Results: the articles identified in this review support the notion that tart cherry consumption attenuates EIMD symptoms after intense exercise bouts. This attenuation seems to be related to the antioxidant and anti-inflammatory properties of anthocyanins and other phenolic compounds present in tart cherries. Conclusion: daily consumption of tart cherries may attenuate inflammatory and oxidative responses to EIMD, leading to faster recovery after exercise bouts (AU)


Introducción: el daño muscular es un fenómeno multifactorial que conduce a la pérdida de la función muscular como resultado de la tensión mecánica e inmune. Este estrés inmunológico es causado principalmente por la inflamación y el estado oxidativo aumentado. Las cerezas son frutas que contienen antocianinas con compuestos fenólicos conocidos, que sirven como pigmento en la naturaleza. Entre tanto, los estudios sugieren que este pigmento puede promover un potente efecto antioxidante y antiinflamatorio cuando se consume por los seres humanos. Objetivos: el objetivo de este estudio fue realizar una revisión crítica de la literatura sobre el consumo de cerezas, con un enfoque en la identificación de las estrategias de protección contra el daño muscular. Métodos: se realizó una encuesta en la base de datos PubMed. Esta revisión presenta los resultados para el consumo de cerezas y daño muscular. Resultados: los artículos que se encuentran en esta revisión apoyan la idea de que el consumo de cerezas alivia los síntomas de daño muscular después de las sesiones de ejercicio. Esta atenuación parece estar relacionada con las propiedades antioxidante y antiinflamatoria de las antocianinas y otros compuestos fenólicos presentes en las cerezas. Conclusión: el consumo diario de cerezas puede aliviar la respuesta inflamatoria y oxidativa de los entrenamientos y conseguir una recuperación más rápida de los marcadores de daño muscular (AU)


Assuntos
Humanos , Prunus , Flavonoides/farmacocinética , Contratura/tratamento farmacológico , Dor Musculoesquelética/tratamento farmacológico , Substâncias Protetoras/farmacocinética , Sistema Musculoesquelético/lesões , Traumatismos em Atletas/prevenção & controle
14.
Nutr. hosp ; 32(4): 1462-1467, oct. 2015.
Artigo em Inglês | IBECS | ID: ibc-143637

RESUMO

The effect of diet on cardiovascular disease prevention has been widely studied for many years. Numerous studies have confirmed that diets rich in fruits and vegetables (Mediterranean diet) are beneficial to the cardiovascular system and various bioactive food components have preventive effect on chronic diseases such as cardiovascular disease. In this paper we review the effect of bioactive substances included in the group of flavonoids (catechins and proanthocyanidins, anthocyanins and isoflavones), stilbenes such as resveratrol, bioactive peptides, plant sterols and polyunsaturated fatty acids omega-3 on the cardiovascular system (AU)


El efecto de la dieta sobre la prevención de las enfermedades cardiovasculares ha sido ampliamente estudiado durante muchos años. Numerosos estudios han corroborado que las dietas ricas en frutas y hortalizas (dieta mediterránea) resultan cardiosaludables y que diversas sustancias bioactivas que componen los alimentos tienen un efecto preventivo en diversas enfermedades crónicas como son las enfermedades cardiovasculares. En esta revisión vamos a tratar ciertas sustancias bioactivas, como son algunas incluidas en el grupo de los flavonoides (catequinas y proantocianidinas, antocianinas e isoflavonas), estilbenos como el resveratrol, péptidos bioactivos, esteroles vegetales y ácidos grasos poliinsaturados omega-3 (AU)


Assuntos
Humanos , Doenças Cardiovasculares/prevenção & controle , Substâncias Protetoras/farmacocinética , Terapia Nutricional/métodos , Flavonoides/farmacocinética , Estilbenos/farmacocinética , Fitosteróis/farmacocinética , Ácidos Graxos Insaturados/farmacocinética
15.
An. R. Acad. Farm ; 81(3): 258-265, jul.-sept. 2015. graf, tab
Artigo em Inglês | IBECS | ID: ibc-146512

RESUMO

Characterization of flavonoids and limonoids in the defatted acetone extract of Khaya senegalensis flowers (A. Juss.) contents was performed using ultra performance liquid chromatography (UPLC) coupled with ultraviolet (UV) and electrospray ionization (ESI) mass spectrometry, furthermore, tandem mass spectrometry (MS/MS) was performed to assist in the structural elucidation. The antimicrobial effect was tested against representative gram positive and negative bacteria and candida. Cytotoxicity of extract was evaluated using the mitochondrial- dependent reduction of MTT. The method used enabled identification of five flavonoid glycosides (di and mono- sugar) and twelve limonoids of different types viz: mexicanolides, phragmalins and angolensate were tentatively identified. The extract was effective against tested microorganism revealing potent growth inhibitory effect on Salmonella typhimurium ATCC 25566, Escherichia coli NRRN 3008 , Pseudomonas aeruginosa ATCC 10145 and fungus Candida albicans EMCC105, MIC ≤ 25µg/µl while MIC ≤ 50 µg/µl for Bacillus cereus, staphylococcus aureus ATCC 6538. Extract showed cytotoxicity against MCF7 (Breast carcinoma cell line) compared to doxorubicin, IC50=88.1(µg/mL) but no activity on HCT 116 (Colon carcinoma cell line) and HepG2 (liver cell carcinoma) was observed. Bioactive compounds in K senegalensis flowers acetone extract possesses promising antimicrobial activity with low cytotoxic effect warrants further investigation for their therapeutic and prophylactic roles


Se ha procedido a la caracterización de los flavonoides y limonoides del extracto acetónico de las flores de la especie Khaya senegalensis (A. Juss.) El análisis de sus componentes se realizó mediante cromatografía líquida de ultra resolución (UPLC) con detección ultravioleta (UV) y de espectrometría de masas de ionización por electrospray (ESI), así como espectrometría de masas (MS / MS) para ayudar en la elucidación estructural de los compuestos. Se comprobó el efecto antimicrobiano en bacterias gram positivas y negativas y levaduras como el género Candida. La citotoxicidad del extracto se evaluó mediante la reducción mitocondrial dependiente de MTT. El método de análisis permitió la identificación de cinco glucósidos flavonoides (di y mono-azúcar) y doce limonoides de diferentes tipos:se identificaron tentativamente mexicanolidos, phragmalinas y angolensato. El extracto fue efectivo contra microorganismos (≤ MIC 25µg / l ), revelando potente efecto inhibidor del crecimiento de Salmonella typhimurium (ATCC 25566), Escherichia coli (NRRN 3008), Pseudomonas aeruginosa (ATCC 10145) y el hongo Candida albicans (EMCC105). Igualmente fue eficaz a MIC ≤ 50 mg / l sobre Bacillus cereus, Staphylococcus aureus (ATCC 6538). Igualmente, el extracto mostró citotoxicidad contra línea celular de carcinoma de mama (MCF7) en comparación con la doxorrubicina, (IC50 = 88.1 (µg/ mL), pero no modificó la actividad en una línea celular de carcinoma colon (HCT 116) y en células de carcinoma del hígado (HepG2). Los compuestos bioactivos en extracto acetónico de flores de K. senegalensis poseen una actividad antimicrobiana prometedora con bajos efectos citotóxicos que garantizan la necesidad de una mayor investigación para conocer completamente sus papeles terapéuticos y profilácticos


Assuntos
Meliaceae , Meliaceae/imunologia , Meliaceae/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Plantas Medicinais/microbiologia , Flavonoides/química , Flavonoides/farmacologia , Testes de Sensibilidade Microbiana , Relação Dose-Resposta a Droga , Cromatografia Líquida de Alta Pressão , Espectrometria de Fluorescência , Produtos com Ação Antimicrobiana
16.
Rev. esp. nutr. comunitaria ; 21(3): 29-37, jul.-sept. 2015. tab
Artigo em Espanhol | IBECS | ID: ibc-163592

RESUMO

El mangostán (Garcinia mangostana L.) es un fruto de origen asiático, perteneciente a la familia de las Gutíferas y considerado en Tailandia como «la reina de las frutas». Sus efectos en beneficio de la salud se atribuyen a las xantonas, compuestos de naturaleza fenólica presentes en la corteza. Varios investigadores, en estudios in vitro e in vivo, han demostrado sus propiedades biológicas, entre las que podemos destacar una importante actividad para combatir especies reactivas de oxígeno/nitrógeno (ROS/RNS), actividad antiinflamatoria, efectos anticancerígenos sobre varias líneas celulares de cáncer (próstata, mama, leucemia, colon, etc.), actividad antimicrobiana, control de síntomas neuropsiquiátricos y cognitivos y mejora de parámetros relacionados con la obesidad. En España, el mangostán está empezando a utilizarse como complemento alimenticio aunque debemos ser cautos, pues son necesarios más ensayos clínicos en humanos para aclarar sus mecanismos de absorción, metabolismo y eliminación en el organismo (AU)


The mangosteen (Garcinia mangostana L.) is a fruit of Asian origin, belonging to the family of Gutifferae and considered in Thailand as the «queen of fruits». Its effects on health benefit are attributed to xanthones, phenolic compounds present in the pericarp. Several researchers, with in vitro and in vivo studies, have proved their biological properties, among which we can highlight an important activity to combat reactive species of oxygen/nitrogen (ROS/RNS), anti-inflammatory activity, anticancer effects on various cancer cell lines (prostate, breast, leukemia, colon, etc.), antimicrobial activity, control of neuropsychiatric and cognitive symptoms and improvement parameters associated with obesity. In Spain, the mangosteen is starting to be used as a dietary supplement but we must be cautious, as we need more clinical trials in human beings to clarify the mechanisms of absorption, metabolism and elimination within the organism (AU)


Assuntos
Humanos , Garcinia mangostana , Anti-Inflamatórios/metabolismo , Medicina Tradicional/métodos , Produtos com Ação Antimicrobiana , Antioxidantes/administração & dosagem , Flavonoides/metabolismo , Obesidade/prevenção & controle
17.
Nutr. hosp ; 31(3): 1163-1170, mar. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-134412

RESUMO

The recent introduction of the cultivation of Steviarebaudiana Bertoni in Mexico has gained interest for its potential use as a non-caloric sweetener, but some other properties of this plant require studies. Extracts from two varieties of S. rebaudiana Bertoni adapted to cultivation in Mexico were screened for their content of some phytochemicals and antioxidant properties. Total pigments, total phenolic and flavonoids contents of the extracts ranged between 17.7-24.3 mg/g, 28.7-28.4 mg/g, and 39.3-36.7 mg/g, respectively. The variety «Criolla» exhibited higher contents of pigments and flavonoids. Trolox equivalent antioxidant capacity ranged between618.5-623.7 mM/mg and DPPH decolorization assay ranged between 86.4-84.3%, no significant differences were observed between varieties. Inhibition of β-carotene bleaching ranged between 62.3-77.9%, with higher activity in the variety «Criolla». Reducing power ranged between 85.2-86% and the chelating activity ranged between57.3-59.4% for Cu2+ and between 52.2-54.4% forFe2+, no significant differences were observed between varieties. In conclusion, the results of this study showed that polar compounds obtained during the extraction like chlorophylls, carotenoids, phenolic compounds and flavonoids contribute to the antioxidative activity measured. The leaves of S. rebaudiana Bertoni could be used not only as a source of non-caloric sweeteners but also naturally occurring antioxidants (AU)


La reciente introducción del cultivo de Stevia rebaudiana Bertoni en México ha ganado interés debido a su potencial uso como fuente de edulcorantes no calóricos, pero otras propiedades de esta planta aun requieren de estudios. Extractos de hojas de dos variedades de S. rebaudiana Bertoni adaptadas al cultivo en México fueron evaluados en cuanto a su contenido de algunos fitoquímicos y sus propiedades antioxidantes. El contenido de pigmentos, fenoles totales y flavonoides en los extractos, osciló entre 17.7-24.3 mg/g, 28.7-28.4 mg/g, y 39.3-36.7 mg/g, respectivamente. La variedad «Criolla» exhibió los mayores contenidos de pigmentos y flavonoides. La capacidad antioxidante equivalente de Trolox osciló entre 618.5-623.7 mM/mg y el ensayo de decoloración del radical DPPH osciló entre 86.4-84.3%, no observándose diferencias significativas entre ambas variedades. La inhibición de la decoloración del β-caroteno osciló entre 62.3-77.9%, siendo mayor en la variedad «Criolla». El poder reductor osciló entre 85.2-86%, las capacidades quelantes de cobre y hierro oscilaron entre 57.3-59.4% y 52.2-54.4%, respectivamente, no observándose diferencias significativas entre ambas variedades. En conclusión, los resultados de este estudio demuestran que los compuestos de naturaleza polar obtenidos durante la extracción, tales como pigmentos clorofílicos, carotenoides, compuestos fenólicos y flavonoides contribuyen a la actividad antioxidante. Las hojas de S. rebaudiana Bertoni podrían ser empleadas no solo como fuente de edulcorantes no calóricos, sino también como fuente de antioxidantes de origen natural (AU)


Assuntos
Humanos , Antioxidantes/isolamento & purificação , Stevia , Extratos Vegetais/farmacocinética , Medicamentos Fitoterápicos , Adoçantes não Calóricos/farmacocinética , Clorofila/farmacocinética , Carotenoides/farmacocinética , Compostos Fenólicos/análise , Flavonoides/farmacocinética
18.
Clin. transl. oncol. (Print) ; 17(2): 145-151, feb. 2015. ilus
Artigo em Inglês | IBECS | ID: ibc-132885

RESUMO

Background. The treatment of castrate-resistant prostate cancer (CRPC) still remains as an important challenge of daily oncology practice. Docetaxel significantly prolongs overall survival in men with CRPC. Thymoquinone (TQ), one of the flavonoid compounds isolated from Nigealla sativa, has been shown to possess cytotoxic activity against a variety of cancer cell lines. Materials and Methods. The aim of the study was to investigate the possible synergistic cytotoxic/apoptotic effects of a novel combination, docetaxel and TQ in DU-145 hormone- and drug-refractory prostate cancer cells and their effects on PI3K and ERK signaling pathways. Results. We observed that the combination of docetaxel and TQ resulted in a significant synergistic cytotoxicy and apoptosis as compared to any single agent alone, in a dose-dependent manner. It was found that viability of the combination treated cells was not significantly changed in the presence of LY294002 as compared to inhibitor treated cells. However, in the presence of FR180204, viability of combination treated cells was significantly decreased as compared to inhibitor treated cells. In conclusion, cytotoxic effect of the docetaxel and TQ combination is correlated with the block of the PI3K/Akt signaling pathway in DU-145 cells. Conclusion. Therefore, this combination strategy may be an alternative approach for the challenging era of daily oncologic practice. Also, the combination of docetaxel and TQ might allow a reduction in docetaxel doses and diminish adverse effects of docetaxel while maintaining the therapeutic effect in patients with CRPC (AU)


No disponible


Assuntos
Humanos , Masculino , Apoptose , Proteína Oncogênica v-akt , Neoplasias da Próstata/complicações , Neoplasias da Próstata/diagnóstico , Flavonoides/uso terapêutico , Proteína Oncogênica v-akt , Proteínas Oncogênicas , Fosfatidilinositóis , Flavonoides/efeitos adversos , Neoplasias da Próstata/induzido quimicamente , Sinergismo Farmacológico , Citotoxicidade Celular Dependente de Anticorpos , Terapia Combinada/métodos
20.
J. physiol. biochem ; 70(4): 891-900, dic. 2014.
Artigo em Inglês | IBECS | ID: ibc-131423

RESUMO

The flavonoid content of tea (Camellia sinensis) has beneficial properties in the prevention of diseases. However, the mechanisms by which white tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 (Nrf2), NAD(P)H:quinone oxidoreductase 1 (Nqo1), glutathione S-transferase (Gst), haem oxygenase-1 (Ho1), catalase (Cat), superoxide dismutase (Sod) and glutathione reductase (Gr) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2, Gst, Nqo1, Ho1, Cat, Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of white tea increased in a higher degree the expression of Nrf2, Gst, Nqo1 and Ho1 in the tea + ADR group compared with the control group and C + ADR group. In addition, tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner (AU)


Assuntos
Animais , Ratos , Camellia sinensis , Chá , Estresse Oxidativo , Flavonoides/farmacocinética , Fator 2 Relacionado a NF-E2/farmacocinética , Polifenóis/farmacocinética , Biologia Molecular/métodos , Doxorrubicina/farmacocinética , Modelos Animais de Doenças , Substâncias Protetoras/farmacocinética
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