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3.
J. physiol. biochem ; 71(4): 635-648, dic. 2015.
Artigo em Inglês | IBECS | ID: ibc-145717

RESUMO

Diabetes mellitus is an ever growing world-wide health problem. The patient has to stick to a firm life-long therapeutic regimen, otherwise diabetic complications will develop. Diabetic nephropathy (DN) is one of the most common diabetic complications and it requires careful medical attendance. Nilotinib hydrochloride is a protein tyrosine kinase inhibitor reported to have numerous therapeutic efficacies besides being an anticancer. In the current study, single I.P. streptozotocin (50 mg/kg) injection was used to induce type I diabetes mellitus in male Sprague–Dawley rats. After 8 weeks, significant deterioration of renal function with urinary excretion of nephrin, podocalyxin, and albumin was observed. Daily oral administration of nilotinib (20 mg/kg) for 8 weeks significantly improved signs of DN on all investigated scales. On a biochemical scale, kidney functions, albuminuria, urinary nephrin, podocalyxin excretion, and host oxidant/antioxidant balance significantly improved. Kidney content of nitric oxide, expression of toll-like receptors 4 and NF-káppaB/p65 activity significantly declined as well. On a histopathological scale, Alpha-smooth muscle actin and nestin expression significantly declined. Meanwhile, area of fibrosis significantly declined as seen with significant reduction in accumulation of extracellular matrix components and kidney content of collagen. Ultimately, such improvements were accompanied by significant restoration of normal kidney physiology and function. In conclusion, nilotinib can hinder progression of DN through various mechanisms. Reduction of oxidative stress, enhancement of host antioxidant defense system, reduction of inflammation, angiogenesis, tissue hypoxia, and pro-fibrogenic biomarker expression can be implicated in the beneficial therapeutic outcome observed with nilotinib therapy (AU)


No disponible


Assuntos
Animais , Ratos , Nefropatias Diabéticas/prevenção & controle , Insuficiência Renal/prevenção & controle , Proteínas Tirosina Quinases/antagonistas & inibidores , Substâncias Protetoras/farmacocinética , Modelos Animais de Doenças , Receptor 4 Toll-Like , Miócitos de Músculo Liso , Actinas , Nestina
4.
Eur. j. anat ; 19(4): 323-330, oct. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-145661

RESUMO

Seven Baladi goats of both sexes (9 to 24 months old) were used to describe the distribution pattern of ki-67, alpha smooth muscle actin (áSMA) and vimentin (VIM) in the reticulum and omasum. This study was carried out using the avidin-biotin immunoperoxidase method. Ki-67 immunostaining was restricted to the basal cells layer in the epithelia of both the reticulum and omasum, suggesting the importance of ki-67 in epithelial cells proliferation and keratin biosynthesis. Immunostaining for áSMA was detected in smooth muscle cells in reticular folds, omasal lamiae and muscularis in both the reticulum and omasum, indicating the critical role of áSMA in muscular motility. The widespread distribution of VIM immunostainings in epithelia, fibroblasts in lamina propria and submucosa, and endothelia of blood vessels supports the importance of VIM as an intermediate filament protein. Detection of VIM in glial cells of enteric plexuses indicates its supportive role in the nervous control of both reticulum and omasum. Overall, this immunohistochemical study revealed non-significant differences in the expression of ki-67, áSMA, and VIM between the reticulum and omasum. This study thus verifies the important roles of ki-67, áSMA and VIM in the structure and function of the reticulum and omasum of Baladi goats


No disponible


Assuntos
Animais , Antígeno Ki-67/análise , Vimentina/biossíntese , Actinas/biossíntese , Retículo/fisiologia , Omaso/fisiologia , Músculo Liso/fisiologia , Cabras/fisiologia , Imuno-Histoquímica/métodos
5.
Rev. esp. cir. oral maxilofac ; 37(2): 108-112, abr.-jun. 2015. ilus
Artigo em Inglês | IBECS | ID: ibc-139757

RESUMO

Low-grade myofibroblastic sarcoma (LGMS) represents an atypical tumor composed of myofibroblasts with a predilection for the head and neck, especially in the tongue and oral cavity, with a high tendency to local recurrences and metastases, even after a long period. LGMS arising in the maxillary sinus and in the neck are extremely uncommon. To the best of our knowledge, only 50 cases of low-grade myofibroblastic sarcoma have been reported. We report two cases of LGMS of the maxillary sinus and neck, discussing clinical, histological, inmunohistochemical and therapeutic features (AU)


El sarcoma miofibroblástico de bajo grado (SMFBG) representa un tumor atípico, formado por miofibroblastos, que tiene predilección por cabeza y cuello, en especial la lengua y la cavidad oral, y se caracteriza por una elevada tendencia a las recidivas locales y a las metástasis, incluso después de transcurrido un período prolongado. Los SMFBG que se originan en el seno maxilar y en el cuello son excepcionales. Hasta lo que conocen los autores, solo se han publicado 50 casos de sarcoma miofibroblástico de bajo grado. Describimos 2 casos en los que se identificaron estos tumores, uno en el seno maxilar y el otro en el cuello, y abordamos sus características clínicas, histológicas, inmunohistoquímicas y terapéuticas (AU)


Assuntos
Idoso , Feminino , Humanos , Miofibroma/patologia , Sarcoma/patologia , Neoplasias Bucais/patologia , Imuno-Histoquímica/métodos , Actinas/análise , Recidiva Local de Neoplasia/patologia
10.
Sanid. mil ; 70(1): 20-24, ene.-mar. 2014. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-122900

RESUMO

INTRODUCCIÓN: La gestión de bancos de germoplasma implica la conservación y uso de dosis seminales, pero también pueden ser una fuente de estudio sobre la calidad de los sementales y las propiedades del semen para su empleo post descongelación. Un criterio para medir la calidad seminal puede basarse en las diferencias de expresión de algunos genes implicados en la espermatogénesis y la maduración espermática. OBJETIVO: Análisis de genes expresados en semen equino criopreservado que ofrezcan una adecuada amplificación, especificidad y estabilidad para su empleo como genes de referencia en futuros estudios de expresión genética. MATERIAL Y MÉTODOS: Purificación de espermatozoides vivos mediante un gradiente de concentración discontinua a partir de pajuelas de semen criopreservado correspondiente a cuatro sementales. Extracción orgánica de ácidos ribonucleicos con tratamiento con la enzima desoxiribonucleasa y la amplificación selectiva de siete genes candidatos mediante retrotranscripción y reacción en cadena de la polimerasa en tiempo real en un solo paso. RESULTADOS: Tres de los genes seleccionados, β-Actina, Ubiquitina B y proteína Ribosomal L32 se amplifican correctamente. β-Actina, Ubiquitina B manifiestan la mayor estabilidad. CONCLUSIÓN: En los espermatozoides procedentes de muestras de semen criopreservado equino se puede detectar la presencia de ARNm, siendo el gen de la β-Actina y de la Ubiquitina B los más indicados como genes de referencia de los siete candidatos analizados


INTRODUCTION: The germoplasm bank management involves the conservation and use of semen doses, but can also be a source of study on the quality of stallions and semen properties for use after thawing. A criterion for measuring the semen quality may be based on differences in expression of some genes involved in spermatogenesis and sperm maturation. OBJECTIVE: Analysis of genes expressed in equine cryopreserved sperm that can provide adequate amplification, specificity and stability for use as future reference genes in gene expression studies. MATERIAL AND METHODS: Purification of live sperm through a discontinuous concentration gradient from cryopreserved semen straws corresponding to four stallions. Organic extraction of ribonucleic acids with deoxyribonuclease treatment and the selective amplification of seven candidate genes using a retrotranscription and a real time chain reaction of the polymerase in one step mode. Specificity is tested by melting curves and agarose gel electrophoresis. Also the stability of the genes is calculated. RESULTS: Three of the selected genes, β-actin, Ubiquitin B and Ribosomal protein L32 were properly amplified. β-Actin and Ubiquitin B showed the best stability. CONCLUSION: mRNA was amplified from equine cryopreserved semen samples, being the β-Actin and the Ubiquitin B genes the most suitable reference genes of the seven candidates analyzed


Assuntos
Animais , Cavalos/genética , Criopreservação , Análise do Sêmen/métodos , Expressão Gênica , Preservação do Sêmen/métodos , Bancos de Esperma , Reação em Cadeia da Polimerase/métodos , RNA Mensageiro/análise , Actinas/genética
11.
Clin. transl. oncol. (Print) ; 16(2): 184-190, feb. 2014. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-127723

RESUMO

OBJECTIVE: The common reference genes of choice in relative gene expression studies based on quantitative real time polymerase chain reaction, ACTB and B2M, were shown to be regulated differently in respect to tissue type. In this study, the stability of the selected housekeeping genes for normalizing the qPCR data were identified in the tumor and its adjacent tissues in invasive breast cancer, and the variability of their levels according to the stages and the histopathologic subtypes was analyzed. METHODS: Four housekeeping genes: PUM1, RPL13A, B2M, and ACTB were analyzed in 99 surgically excised tissue specimens (50 tumor, 45 tumor adjacent and 4 normal breast tissues). Three of the most common softwares (GeNorm, NormFinder, and BestKeeper) were used for calculation purposes. RESULTS: When all of the tissue samples were included in analyses, PUM1 was the most stable gene according to calculations made with both NormFinder and BestKeeper; while PUM1/RPL13A combination was the most stable by GeNorm software. The PUM1 gene was also identified as the most stable gene among the four in all sample groups (in both Estrogen Receptor positive and Estrogen Receptor negative subgroups of invasive breast carcinoma and in normal breast tissue) according to calculations made using the NormFinder software. CONCLUSION: While suggesting PUM1 is one of the most stable single gene and the PUM1/RPL13A pair as one of the best housekeeping genes for the normalization of expression studies in invasive breast tumor studies, it will be more practical to evaluate stability once more and decide upon the reference gene accordingly within the sample group itself (AU)


No disponible


Assuntos
Humanos , Feminino , Neoplasias da Mama/genética , Perfilação da Expressão Gênica/normas , Genes Essenciais , Reação em Cadeia da Polimerase Via Transcriptase Reversa/normas , Actinas/genética , Neoplasias da Mama/epidemiologia , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica , Estabilidade de RNA , Proteínas de Ligação a RNA/genética , Padrões de Referência , Proteínas Ribossômicas/genética , Microglobulina beta-2/genética
13.
Clín. investig. arterioscler. (Ed. impr.) ; 24(3): 141-146, mayo-jun. 2012. ilus
Artigo em Espanhol | IBECS | ID: ibc-105088

RESUMO

Los inhibidores de la 3-HMG-CoA-reductasa (estatinas) son fármacos cuyos beneficios clínicos en la prevención de las enfermedades cardiovasculares están ampliamente demostrados. Una parte de tales beneficios, y particularmente los que pueden tener lugar de forma más inmediata, se deben a efectos que pueden ser independientes del descenso de los niveles de colesterol y de cLDL. Estos efectos se conocen como efectos pleiotrópicos. En el presente trabajo, mediante cultivos de células implicadas en el proceso aterogénico —fibroblastos, monocitos, células endoteliales y células musculares lisas— sometidos a distintos tipos y dosis de estatinas, hemos intentado evidenciar los cambios en el comportamiento celular. Nuestros resultados apoyan la idea de que se producen cambios subsiguientes a la adición de las estatinas en el medio de cultivo, y que afectan tanto a la adhesividad celular como a la movilidad y —en el caso de las células musculares lisas— a la contractilidad. Estos cambios pueden explicar, al menos en parte, algunos de los efectos distintos a los directamente relacionados con el efecto hipocolesterolemiante. En el fondo de tales cambios se pueden encontrar modificaciones en el metabolismo intracelular (AU)


In the present study, using cultures of the cells involved in the atherogenic process: fibroblasts, monocytes, endothelial cells and smooth muscle cells submitted to different types and doses of statins, we have attempted to demonstrate the changes in cell behaviour. Our results support the idea that subsequent changes are produced on the addition of statins into the culture medium, and which affect cell adhesiveness, as well as mobility and. in the case of smooth muscle cells, the contractility. These changes may explain, at least partly, some of the effects other than those directly related to the lipid lowering effect. Behind such changes may be found alterations in intracellular metabolism.3-HMG-CoA-reductase inhibitors (statins) are drugs which have been widely demonstrated to have clinical benefits in the prevention of cardiovascular diseases. Some of these benefits, particularly those that may take place immediately, are due to effects that may be independent of the decrease in cholesterol and LDL-cholesterol levels. These effects are known as pleiotropic effects (AU)


Assuntos
Humanos , Inibidores de Hidroximetilglutaril-CoA Redutases/farmacocinética , Actinas/fisiologia , Aterosclerose/fisiopatologia , Anticolesterolemiantes/farmacocinética , Microscopia , Fibroblastos
14.
Rev. neurol. (Ed. impr.) ; 52(12): 720-724, 16 jun., 2011. ilus
Artigo em Espanhol | IBECS | ID: ibc-91665

RESUMO

Introducción. La distrofia muscular de Duchenne (DMD) es un trastorno caracterizado por atrofia muscular progresiva y debilidad, debido a la ausencia o alteración de la función de la distrofina, una proteína que protege a las células del músculo de la tensión mecánica inducida durante la contracción. Las mutaciones en el gen DMD pueden dar lugar a diferentes fenotipos clínicos, conocidos colectivamente como distrofinopatías, de los cuales la DMD es el más grave. Caso clínico. Se presenta una deleción nueva de los exones 24-41, que no interrumpe el marco de lectura y se espera que origine un fenotipo leve. Por el contrario, el paciente tiene un fenotipo DMD grave. Conclusiones. Nuestra comunicación apoya la hipótesis de que la interrupción del sitio de unión a gamma-actina situado en el dominio cilíndrico central desempeña un papel crucial en la función de amortiguador de distrofina en las células musculares. La descripción de las variantes patogénicas en el gen DMD y los fenotipos resultantes tienen importantes implicaciones en el diseño de estrategias terapéuticas moleculares para la DMD (AU)


Introduction. Duchenne muscular dystrophy (DMD) is a genomic disorder characterized by progressive muscle wasting and weakness due to the absence or abnormal function of dystrophin; a protein that protects muscle cells from mechanical induced stress during contraction. Mutations in the DMD gene, may lead to different clinical phenotypes, collectively known as dystrophinopathies, of which DMD has the earliest onset and most severe progression. Case report. We report a novel deletion of exons 24-41, predicted to maintain the reading frame and expected to result in a mild phenotype. Conversely, the patient has a severe DMD phenotype. Conclusions. Our report supports the hypothesis that disruption of the gamma-actin-binding site located in the central rod domain plays a crucial role in the shock absorber function of dystrophin in muscle cells. Description of pathogenic variants in the DMD gene and the resulting phenotypes has important implications on the designing of molecular therapeutic approaches for DMD (AU)


Assuntos
Humanos , Masculino , Distrofia Muscular de Duchenne/genética , Fenótipo , Genótipo , Actinas/genética , Sítios de Ligação/genética
15.
Arch. Soc. Esp. Oftalmol ; 85(12): 400-404, dic. 2010. ilus
Artigo em Espanhol | IBECS | ID: ibc-88239

RESUMO

Propósto: La destrucción de la barrera del epitelio limbal es el mecanismo más importante en la formación del pterygium. Se piensa que proviene de la activación y proliferación de células madre (stem cells) del epitelio limbal. El objetivo de este estudio ha sido determinar la presencia de células mesenquimíticas indiferenciadas (stem cells) en cultivos celulares obtenidos de pterygium humano. Métodos: Las células de 6 pterygium humanos fueron aisladas mediante explantos y cultivadas en amniomax. Al alcanzar la confluencia, las células fueron sembradas en microplacas de 24 pocillos. Los cultivos celulares fueron estudiados en el 2.¡- subcultivo. Se analizá mediante inmunofluorescencia la expresión de los marcadores de células madre embrionarias, OCT3/4 y CD9. Los diferentes fenotipos celulares fueron caracterizados con los anticuerpos monoclonales anti-CD31, Á-actina y vimentina. Resultados: Toda la población celular obtenida de pterygium muestra expresión para vimentina. Menos de un 1% de las células presentaron expresión para los marcadores CD31 y Á-actina. La mayor parte de la población celular fue positiva para los marcadores embrionarios OCT3/4 y CD9. Conclusión: La población celular expandida a partir de pterygium está compuesta por células de fenotipo mesenquimal, que muestran expresión de marcadores embrionarios OCT3/4 y CD9. Esta población, con un alto grado de indiferenciación, podrá ser responsable de la alta tasa de recurrencia del pterygium tras su extirpación quirurgica (AU)


BackgroundDestruction of the limbal epithelium barrier is the most important mechanism of pterygium formation (conjunctiva proliferation, encroaching onto the cornea). It is thought to arise from activated and proliferating limbal epithelial stem cells. The objective of this study is to evaluate the presence of undifferentiated mesenchymal cells (stem cells) in cultured cells extracted from human pterygium.Material and methodsCells from 6 human pterygium were isolated by explantation and placed in cultures with amniomax medium. Once the monolayer was reached the cells were seeded onto 24 well microplates. The cells were studied in the second sub-culture. The immunohistochemical expression of different embryonic stem cell markers, OCT3/4 and CD9, was analysed. The differentiated phenotypes were characterised with the monoclonal antibodies anti-CD31, ¦Á-actin and vimentin.ResultsAll the cell populations obtained from pterygium showed vimentin expression. Less than 1% of the cells were positive for CD31 and ¦Á-actin markers. The majority of the cell population was positive for OCT3/4 and CD9.ConclusionsThe cell population obtained from pterygium expressed mesenchymal cell phenotype and embryonic markers, such us OCT3/4 and CD9. This undifferentiated population could be involved in the large recurrence rate of this type of tissue after surgery(AU)


Assuntos
Humanos , Pterígio/patologia , Expressão Gênica , Células-Tronco Mesenquimais , Biomarcadores/análise , Actinas/análise , Molécula-1 de Adesão Celular Endotelial a Plaquetas/isolamento & purificação
16.
Int. microbiol ; 12(4): 243-251, dic. 2009. tab
Artigo em Inglês | IBECS | ID: ibc-77877

RESUMO

A collection of 69 eae-positive strains expressing 29 different intimin types and eight tir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BFP) expression. All of the nine typical enteropathogenic Escherichia coli (tEPEC) studied harbored the enteropathogenic E. coli adherence factor (EAF) plasmid, as shown by PCR and/or EAF probe results. In addition, they were positive for bfpA, as shown by PCR, and BFP expression, as confirmed by immunofluorescence (IFL) and/or immunoblotting (IBL) assays. Localized adherence (LA) was exclusively displayed by those nine tEPEC, while localized-adherence-like (LAL) was the most frequent pattern among atypical EPEC (aEPEC) and Shiga-toxinproducing E. coli (STEC). All LA and LAL strains were able to cause attaching and effacing (AE) lesions, as established by means of the FAS test. There was a significant association between the presence of tir allele alpha1 and bfpA-positive strains, and consequently, with the LA pattern. However, intimin type or bfpA was not associated with the adherence pattern displayed in HeLa cells. Among the eight bfpA alleles detected, a new type (beta10; accession number FN391178) was identified in a strain of serotype O157:H45, and a truncated variant (beta3.2-t; accession number FN 391181) in four strains belonging to different pathotypes (AU)


No disponible


Assuntos
Humanos , Aderência Bacteriana , Actinas/metabolismo , Escherichia coli Enteropatogênica/patogenicidade , Escherichia coli Shiga Toxigênica/patogenicidade , Alelos , Fímbrias Bacterianas/fisiologia , Proteínas de Escherichia coli/genética , DNA Bacteriano/genética , Genótipo , Células HeLa , Microscopia de Fluorescência , Reação em Cadeia da Polimerase , Plasmídeos
17.
Rev. esp. enferm. dig ; 101(5): 336-342, mayo 2009. tab
Artigo em Espanhol | IBECS | ID: ibc-74399

RESUMO

Chronic intestinal pseudoobstruction (CIPO) is a rare entitycharacterized by recurrent clinical episodes of intestinal obstructionin which no mechanical cause is identified. There are multiplecauses for this syndrome but two main groups can be distinguished:a) secondary to a systemic non-gastrointestinal disease;and b) primary or idiopathic originated from alterations in thecomponents of the intestinal wall. The latter forms are the mostuncommon and their diagnosis is generally difficult. In the presentarticle, we describe nine patients with CIPO that were diagnosedin our center over the last six years. Four of them were diagnosedwith primary or idiopathic form of CIPO and another four wereclearly secondary to a systemic disease. The ninth case, whichwas initially diagnosed as secondary, is probably also a primaryform of the disease. The number of patients diagnosed in our center,even thought small, makes us to hypothesize that the prevalenceof CIPO is probably greater than is generally believed andthat the reasons of its rarity are the incomplete understanding ofits physiopathology and the difficulties to achieve a correct diagnosis(AU)


Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Pseudo-Obstrução Intestinal/diagnóstico , Músculo Liso/fisiopatologia , Trânsito Gastrointestinal , Ileostomia/métodos , Doenças Neuromusculares/complicações , Escleroderma Sistêmico/complicações , Actinas/deficiência , Doença Crônica , Colectomia/métodos , Constipação Intestinal/etiologia , Pseudo-Obstrução Intestinal/epidemiologia , Pseudo-Obstrução Intestinal/fisiopatologia , Pseudo-Obstrução Intestinal/cirurgia , Transtornos Puerperais/etiologia , Laparoscopia/métodos , Manometria/métodos
18.
Rev. esp. enferm. dig ; 100(12): 752-757, dic. 2008. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-71083

RESUMO

Introducción: el receptor del factor de crecimiento epidérmico,EGFR(HER-1), es un receptor de tirosina quinasas cuya activaciónpermite un aumento de la proliferación celular, angiogénesis,proceso metastásico y disminución de la apoptosis celular. Nuestroobjetivo es conocer el valor pronóstico de la inmunotinción deEGFR en tumores estromales gastrointestinales (GIST).Pacientes y método: estudio retrospectivo que incluye todoslos GIST intervenidos quirúrgicamente entre 1995-2007 en elServicio de Cirugía General y del Aparato Digestivo del HospitalGeneral de Ciudad Real. Variables clínicas: edad, sexo, clínica,mortalidad, recidiva. Variables patológicas: a) macroscópicas: localización,diámetro; b) microscópicas: necrosis tumoral, índicemitótico, tipo celular; y c) inmunohistoquímicas: vimentina (V9,Dako A/s); actina del músculo liso (HHF-35, Biogenex); CD34(QBEND/10); S100 (Policlonal Dako A/S); CD117 (c-kit Rabbit,antihuman polyclonal antibody, 1:600); PDGFR-alfa (Rabbitpolyclonal antibody, 1:50, Sta. Cruz Biotechnology). Variablesmoleculares pronósticas: P-53, PAb240 (DakoCytomation), 1:75,Ki-67, clona MIB1 (Dako), 1:120 y EGFR pharmDx™ Dako Autostainer(Dako, Dinamarca). Criterios de malignidad: criteriosde Fletcher.Resultados: entre 1995 y 2007, 35 GIST, fueron intervenidosquirúrgicamente en nuestro Servicio. Edad media: 61,11 ±11,02, siendo mujeres en el 62,9% de los casos. Debutaron conhemorragia digestiva en un 40%. La mediana de seguimiento fuede 28 meses (3-133). La mortalidad fue de 54,3%, con recidivadel 40%. Variables morfológicas: la localización más frecuente fuegástrica, 51,4% (18). Existió necrosis tumoral en un 57,1%, 20.El patrón celular fue fusocelular en un 57,1%, y epitelioide en un14,3%. El diámetro máximo fue de 9,58 ± 6,29. El índice mitóticopor 50 campos de gran aumento fue de 13,44 ± 16,08. En un51,45%, 18, fueron neoplasias de alto riesgo. Valores inmunohistoquímicos:CD117+, 85,7%. PDGFRA+, 85,7%. CD34+,77,1%. EGFR+, 62,9%. S100+, 34,3%. Actina+, 20%. Vimentina+,100%. p53+, 40%. ki67+, 10,71 ± 10,82. La expresión deEGFR no se relacionó con la recidiva y/o mortalidad del enfermo p = 0,156, y p = 0,332, respectivamente. El índice mitótico serelacionó con la mortalidad del enfermo, p = 0,02, y recidiva neoplásica,p = 0,013.Conclusión: en nuestra muestra no existió relación entre lainmunotinción de EGFR y el pronóstico del tumor estromal gastrointestinal


Introduction: the epidermal growth factor receptor, EGFR(HER-1), is a tyrosine kinase receptor. EGFR activation plays animportant role in increased cell proliferation, angiogenesis, anddecreased apoptosis. Our objective was to study EGFR immunoexpressionin GIST, as well as its prognostic value.Patients and method: a retrospective study that included allpatients operated on with a histologic diagnosis of GIST at Departmentof Surgery, Hospital General, Ciudad Real, between1995 and 2007. Clinical features: age, sex, manifestations, mortality,recurrence. Pathological features: origin, size, tumoralnecrosis, mitotic index, cell type. Immunohistochemical features:vimentin, (V9, Dako A/s); smooth muscle actin (HHF-35,Biogenex); CD34 (QBEND/10); S100 (Policlonal Dako A/S),CD117, (c-kit Rabbit, antihuman polyclonal antibody, 1:600);PDGFR-alfa (Rabbit polyclonal antibody, 1:50, Sta. Cruz Biotechnology).Prognostic molecular features: P-53, PAb240 (DakoCytomation)1:75; Ki-67, clona MIB1 (Dako), 1:120 y (EGFR)pharmDx™ Dako Autostainer (Dako, Denmark). Malignancycritera: Fletcher's critera.Results: from 1995 to 2007, 35 GISTs were resected in ourDepartment. Mean age: 61.11 ± 11.02, with a female predominanceof 62.9%. Initial clinical manifestation included digestivehemorrhage in 40%. Median follow-up was 28 months (3-133).Mortality was 54.3%, and recurrence rate was 40%. The mostfrequent origin was the stomach, 51.4%, (18). There was tumornecrosis in 57.1% (20). There were spindle-like cells in 57.1%,and epithelioid cells in 14.3%. Mean size was 9.58 ± 6.29. Mitoticindex per 50 high-power fields was 13.44 ± 16.08; 51.45%(18) were high-risk tumors. Immunohistochemical expression:CD117+, 85.7%. PDGFRA+, 85.7%. CD34+, 77.1%. EGFR+,62.9%. S100+, 34.3%. Actin+, 20%. Vimentin+, 100%. p53+,40%. ki67+, 10.71 ± 10.82. There was no correlation betweenEGFR expression and recurrence and/or mortality, p = 0.156and p = 0.332, respectively. Mitosis index related to mortality, p= 0.02, and recurrence, p = 0.013. Conclusion: in our study there was no relation betweenEGFR immunohistochemical expression and the prognosis of GIST (AU)


Assuntos
Receptores de Fatores de Crescimento/análise , Neoplasias Gastrointestinais/química , Neoplasias Gastrointestinais/mortalidade , Imuno-Histoquímica , Prognóstico , Estudos Retrospectivos , Vimentina/análise , Actinas/análise , Recidiva Local de Neoplasia
19.
Av. odontoestomatol ; 24(1): 69-80, ene.-feb. 2008. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-62948

RESUMO

Estudios recientes han demostrado la capacidad del organismo humano para detener el crecimiento de potenciales células cancerígenas y paralizarlas. Este mecanismo antitumoral, que actúa como freno del proceso maligno, se conocía ya en estudios de laboratorio “in vitro”, pero se ha comprobado también su presencia en modelos “in vivo”, tanto en ratones como en muestras de tejido de pacientes con cáncer. A este mecanismo se le denomina senescencia celular y se define como un sistema de defensa de emergencia de las células que están en camino de convertirse en cancerosas, una respuesta ante el estímulo de un oncogén. Se trataría pues de un freno a la progresión de las lesiones cancerizables, condenando a esas células a una “cadena perpetua celular”. Este artículo de revisión se propone describir este mecanismo y poner al día la evidencia al respecto de este proceso, así como los marcadores de senescencia existentes en relación con el cáncer y precáncer oral This article reviews the paper of oral biopsy on the precancerous lesions diagnosis and on the oral cancer early diagnosis. Different techniques, procedures, materials, indications and other surgical aspects are debated. It proposes to do incisional biopsies on malignant lesions and on malignant suspicious lesions, while doing excisional biopsies on precancerous lesions when the size allows it ( AU)


Recent studies have demonstrated the capacity of the human organism to prevent the growth of potentially carcinogenic cells, paralyzing them. This antitumor mechanism, which acts as a brake on the malignant process, was already known in lab studies “in vitro” but has now also been verified “in vivo” in mice and in tissue samples from cancer patients. This mechanism is known as cellular senescence and is defined as an emergency defense system for cells on the way to becoming cancerous, i.e., a response to the stimulation of an oncogene. These cells are sentenced to “life imprisonment”, impeding the progression of premalignant lesions. This review aims to describe this mechanism and present an update of the evidence on this phenomenon in the setting of oral cancer and precance This article reviews the paper of oral biopsy on the precancerous lesions diagnosis and on the oral cancer early diagnosis. Different techniques, procedures, materials, indications and other surgical aspects are debated. It proposes to do incisional biopsies on malignant lesions and on malignant suspicious lesions, while doing excisional biopsies on precancerous lesions when the size allows it (AU)


Assuntos
Senescência Celular/imunologia , Senescência Celular/fisiologia , Biomarcadores/análise , Neoplasias Bucais/diagnóstico , Senescência Celular , Senescência Celular/genética , Telomerase/análise , Telomerase , Heterocromatina , Heterocromatina/patologia , Actinas/análise
20.
Actas urol. esp ; 30(6): 638-640, jun. 2006. ilus
Artigo em Espanhol | IBECS | ID: ibc-048182

RESUMO

Se presenta un caso de leiomiosarcoma de escroto en un paciente de 87 años que debuta como una lesión en hemiescroto izquierdo indolora, y de un año de evolución. En el momento del diagnóstico se observa la presencia de metástasis óseas. Se realiza una revisión de la literatura comprobándose la rareza de este tipo de lesión (sólo se han descrito 27 casos), y que a diferencia de otro tipo de leiomiosarcomas, su evolución agresiva no es habitual en esta localización


A case of scrotum leiomyosarcoma is presented in a 87 year-old patient. It debuts as a painless lesion in left hemiescrotum, of one year of evolution. Bony metástasis were observed in the moment of the diagnosis. We carried out a literature revision proving the rarity of this lesion type (only 27 cases have been described), and that, contrary to another leiomiosarcomas type, their aggressive evolution is not habitual in this localization


Assuntos
Masculino , Idoso , Humanos , Leiomiossarcoma/complicações , Leiomiossarcoma/diagnóstico , Leiomiossarcoma/terapia , Tomografia Computadorizada de Emissão/métodos , Vimentina , Desmina , Actinas , Orquiectomia/métodos , Metástase Neoplásica/patologia , Metástase Neoplásica/fisiopatologia , Leiomiossarcoma/patologia , Leiomiossarcoma/fisiopatologia , Escroto/patologia , Escroto , Condrossarcoma Mesenquimal/complicações , Neoplasias Ósseas/complicações , Neoplasias Testiculares/complicações , Neoplasias Testiculares/diagnóstico
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