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1.
Allergol. immunopatol ; 47(1): 12-15, ene.-feb. 2019. graf
Artigo em Inglês | IBECS | ID: ibc-180765

RESUMO

Introduction and objectives: Profilin is a panallergen contained in pollen, plant foods and latex. Although cross-reactivity is expected while performing skin prick tests (SPT) with allergens that contain profilin, this is not always noticed. The purpose of this study was to detect if profilin is contained in the commercial SPT extracts of pollen and plant foods which, in their fresh form, contain determined epitopes of profilin. Material and methods: Commercial SPT extracts of different pharmaceuticals were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The study included purified palm date profilin, peach (whole, pulp and peel extracts), hazelnut, Olea europea, Parietaria judaica and Phleum pratense. Results: Profilin was detected in all, but peach extracts; it was neither contained in the whole peach extract nor in the ones of peel or pulp. Conclusion: The only accurate way to detect sensitization to profilin, while performing SPT, is the use of purified profilin extract. Even if a plant food or pollen contain an identified molecule of profilin, the relevant SPT commercial extract may not


No disponible


Assuntos
Humanos , Alérgenos/metabolismo , Antígenos de Plantas/metabolismo , Hipersensibilidade/diagnóstico , Extratos Vegetais/metabolismo , Erros de Diagnóstico/prevenção & controle , Profilinas/metabolismo , Testes Cutâneos/métodos , Alérgenos/imunologia , Antígenos de Plantas/imunologia , Reações Cruzadas , Frutas/imunologia , Olea/imunologia , Parietaria/imunologia , Extratos Vegetais/imunologia , Pólen/imunologia , Profilinas/imunologia , Prunus persica/imunologia
2.
J. investig. allergol. clin. immunol ; 28(1): 1-12, 2018. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-171200

RESUMO

Profilin is a protein that is present in all eukaryotic cells and is responsible for cross-reactivity between pollen, latex, and plant foods. It has been classically acknowledged as a minor or nearly irrelevant allergen, although recent data are changing this conception. The objective of this manuscript is to provide a comprehensive review of published data on the role of this ubiquitous allergen in pollen, latex, and plant food allergy. The patterns of recognition of this minor allergen follow a north-south gradient. Although present in all pollens and vegetables, profilin is significantly associated with allergy to grass pollen and to Cucurbitaceae fruits. Heb v 8, the latex profilin, is usually a marker of profilin allergy in plant food-allergic patients, although it has no clinical relevance in latex allergy. Sensitization to profilin jeopardizes the diagnosis of pollen allergy and selection of immunotherapy, and although component-resolved diagnosis can identify its impact, there are no tailored treatments available. In recent years, several new publications have shown how profilin should be taken into account and, under certain circumstances, considered a marker of severity, an allergen capable of inducing respiratory symptoms, and, in its natural purified form, a potential candidate for etiological treatment of food allergy. Current data on profilin strongly support the need for a shift in the previously accepted paradigm for this allergen. More research should be done to assess the real clinical impact of sensitization in specific populations and to develop therapeutic strategies (AU)


La profilina es una proteína presente en todas las células eucariotas, siendo responsable de la reactividad cruzada entre polen, látex y alimentos vegetales. Ha sido reconocida clásicamente como un alérgeno menor o irrelevante; sin embargo, datos recientemente publicados están modificando esta interpretación. El objetivo de este manuscrito es realizar una revisión comprensiva de la literatura sobre el papel de este ubicuo alérgeno en el polen, látex y los alimentos vegetales. El patrón de reconocimiento de este alérgeno menor sigue un gradiente de norte a sur, y a pesar de estar presente en todos los pólenes y vegetales, está significativamente asociado al polen de gramíneas y a las frutas de la familia Cucurbitaceae. Heb v 8, la profilina del látex, es habitualmente un marcador de alergia a profilina en pacientes alérgicos a vegetales pero sin relevancia clínica en la alergia a látex. La presencia de la sensibilización a profilina dificulta el diagnóstico de alergia a pólenes y la selección de la inmunoterapia, y a pesar de que el diagnóstico por componentes puede identificar su impacto, no existen tratamientos personalizados disponibles. En los últimos años, diversas publicaciones nuevas han demostrado como la profilina debe ser tenida en cuenta y considerada bajo determinadas circunstancias, como un marcador de gravedad, como un alérgeno capaz de inducir síntomas respiratorios, y en su forma natural purificada, como un potencial candidato para realizar un tratamiento etiológico para tratar la alergia a alimentos. El conocimiento actual sobre la profilina impulsa la necesidad de cambiar el concepto que previamente se tenía sobre este alérgeno. Sería preciso investigar más para valorar el impacto clínico real de esta sensibilización en poblaciones específicas y desarrollar estrategias terapéuticas (AU)


Assuntos
Humanos , Profilinas/análise , Alérgenos/análise , Dessensibilização Imunológica , Rinite Alérgica Sazonal/imunologia , Hipersensibilidade ao Látex/imunologia , Hipersensibilidade Alimentar/imunologia , Asma/imunologia
4.
J. investig. allergol. clin. immunol ; 27(2): 98-103, 2017. tab
Artigo em Inglês | IBECS | ID: ibc-162317

RESUMO

Background and Objectives: Lipid transfer protein (LTP) sensitization is the most common cause of food allergy in the Mediterranean area, with peach allergy acting as the primary sensitizer in most cases. Lettuce has been described as a common offending food in patients with LTP syndrome. The aim of the study was to investigate the frequency and clinical expression of LTP syndrome in a sample of lettuceallergic patients. Methods: We determined specific IgE to Pru p 3 and lettuce in a sample of 30 patients with a diagnosis of lettuce allergy. Symptoms elicited by other LTP-containing plant-derived foods and the presence of cofactors were assessed. Results: The clinical symptoms of lettuce allergy were frequently severe, with 18 of the 30 patients experiencing anaphylaxis. All the patients had allergic reactions to other plant foods. Cofactors were involved in the clinical reactions of 13 of the 30 patients. Sensitization to pollens was found in 90% of patients. Conclusions: Lettuce allergy is found not as an isolated condition but in the context of LTP syndrome and it is characterized by severe reactions and frequent cofactor association (AU)


Introducción y Objetivo: La sensibilización a la LTP es la causa más frecuente de alergia alimentaria en el área mediterránea, siendo la alergia al melocotón el sensibilizador primario en la mayoría de los casos. La alergia a la lechuga ha sido descrita como un manifestación frecuente en los pacientes que sufren síndrome de LTP. El objetivo del estudio fue investigar la frecuencia del síndrome de LTP en una muestra de pacientes alérgicos a lechuga y evaluar su patrón clínico. Métodos: Se determinó la IgE específica a Pru p 3 y a lechuga en una muestra de 30 pacientes con un diagnóstico de alergia a la lechuga. Se evaluaron los síntomas con otras LTPs de alimentos de origen vegetal y la presencia de cofactores. Resultados: Los síntomas clínicos de la alergia a lechuga fueron frecuentemente graves, ya que 18/30 pacientes experimentaron anafilaxia. Todos los pacientes experimentaron reacciones alérgicas a otros alimentos vegetales. En 13/30 pacientes, los cofactores estaban implicados en las manifestaciones clínicas. Se observó que el 90% de los pacientes estaban sensibilizados a pólenes. Conclusiones: La alergia a la lechuga más que de forma aislada, ocurre en el contexto del síndrome LTP y se caracteriza por su frecuente asociación a cofactores y la gravedad de sus reacciones (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Hipersensibilidade Alimentar/imunologia , Alface/efeitos adversos , Alface/imunologia , Imunoglobulina E/análise , Técnicas In Vitro , Imunização/tendências , Profilinas/isolamento & purificação , Alérgenos/imunologia , Dessensibilização Imunológica/métodos
5.
Allergol. immunopatol ; 44(2): 160-166, mar.-abr. 2016. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-150663

RESUMO

Background: Pollens represent a rich source of proteins that are also potential elicitors of IgE-mediated pollen allergy. Sensitisation to panallergens could play an important role in diagnosis and specific immunotherapy, because these molecules are present in different plant pollens and plant foods and have marked structural similarity in different species. Profilins are one of the most common panallergens to be studied because they are responsible for a large number of sensitisations and are clearly related to cross-reactivity and co-sensitisation. This study aimed to isolate and characterise a new allergen of Amaranthus palmeri pollen and to determine its allergenicity. Methods: A. palmeri pollen profilin was purified using poly-l-proline-Sepharose affinity chromatography followed by anion exchanger chromatography. Identification of purified protein was carried out by mass spectrometry. Specific IgE was estimated in sera of patients with positive skin prick test to A. palmeri pollen extract, by enzyme-linked immunosorbent assay (ELISA). Principal findings: Purified protein appeared as a single band at 14 kDa in SDS-PAGE gel. Mass spectrometric analysis of the gel band identified two highly conserved peptides corresponding to allergenic profilins from pollen of other plants. Sera from about 60% of allergic patients have IgE that recognises the purified A. palmeriprotein. Conclusion: A 14 kDa protein of A. palmeri pollen was purified and identified as allergenic profilin, which was recognised by sera from pollen allergic patients (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Rinite Alérgica Sazonal/diagnóstico , Rinite Alérgica Sazonal/prevenção & controle , Profilinas/isolamento & purificação , Imunoglobulina E/sangue , Cromatografia de Afinidade , Espectrometria de Massas
6.
Rev. clín. med. fam ; 9(1): 60-62, feb. 2016.
Artigo em Espanhol | IBECS | ID: ibc-153708

RESUMO

Presentamos el caso de una paciente atendida en urgencias del centro de salud por un cuadro clínico de shock anafiláctico desencadenado tras la ingesta de manzana y realización posterior de ejercicio físico. En alergia a alimentos, hay que tener presente que en algunas personas es necesaria la presencia de determinados cofactores como el ejercicio físico o la ingesta de determinados fármacos como los AINEs para que suceda una reacción alérgica. Se sospecha que el mecanismo responsable consiste en que aceleren la absorción del alimento en el intestino y así lleguen a modular la severidad de los síntomas. Por este motivo cuando las LTP (proteína de transferencia de lípidos) están implicadas, si los cofactores no se detectan y previenen, pueden representar un serio riesgo para el desarrollo de episodios de anafilaxia severos o fatales (AU)


We report the case of a patient treated in an emergency department of a health center for a clinical picture of anaphylactic shock triggered after ingestion of apple and subsequent practice of physical exercise. In food allergy, it must be remembered that in some people the allergic reaction requires the presence of certain cofactors, such as physical exercise or use of certain prescription drugs such as NSAIDs. It is suspected that the mechanism responsible for this consists in accelerating the absorption of food in the intestine and thereby modulating the severity of symptoms. That is why when lipid transfer proteins (LTP) are involved, if the cofactors are not detected and prevented, they can pose a serious risk for developing severe or fatal episodes of anaphylaxis (AU)


Assuntos
Humanos , Feminino , Adolescente , Anafilaxia/complicações , Anafilaxia/diagnóstico , Anafilaxia/etiologia , Malus/efeitos adversos , Hipersensibilidade Alimentar/diagnóstico , Hipersensibilidade Alimentar/tratamento farmacológico , Proteínas Ligadas a Lipídeos/análise , Profilinas/administração & dosagem , Profilinas/análise , Anafilaxia/fisiopatologia , Anafilaxia/terapia , Exercício Físico , Epinefrina/uso terapêutico , Corticosteroides/uso terapêutico , Antagonistas dos Receptores Histamínicos/uso terapêutico
7.
J. investig. allergol. clin. immunol ; 25(4): 283-287, 2015. tab
Artigo em Inglês | IBECS | ID: ibc-138424

RESUMO

Objetivo: Comparar las pruebas cutáneas prick (PC) con técnicas in vitro (fluoro enzimoinmunoensayo –FEIA- en detección única y múltiple) para detectar sensibilización a profilina y a LTP. Métodos: Se estudiaron retrospectivamente 181 pacientes con alergia a polen y a alimentos vegetales y 61 controles. Se realizaron PC frente a profilina de palmera (Pho d 2) y LTP de melocotón (Pru p 3) y se analizó la IgE específica a Phl p 12 y Pru p 3 por FEIA y por micromatriz de proteínas alergénicas. Resultados: Quince de los 201 sujetos con PC negativa a LTP mostraron ensibilización a este alérgeno mediante IgE específica sérica y en 18 de 41 con PC positivas a LTP no se observó esta sensibilización por otras técnicas. Diecisiete de los 186 sujetos con PC negativa a profilina detectaron IgE específica sérica frente a Phl p 12 y en 30 de los 56 con PC positiva a profilina no se objetivó sensibilización a Phl p 12 en suero. Se observó un acuerdo moderado entre las tres técnicas estudiadas. Conclusiones: La PC frente e a LTP y profilina es un método sensible detectando estas sensibilizaciones y muestra un acuerdo aceptable con las técnicas in vitro, especialmente en los pacientes con negatividad de la PC frente a LTP y a profilina (AU)


Objective: To compare the skin prick test (SPT) with in vitro techniques (single and multiplex fluorescence enzyme-immunoassay [FEIA]) for detecting sensitization to profilin and lipid transfer protein (LTP). Methods: We retrospectively studied 181 patients with pollen and/or plant food allergy and 61 controls. SPT was performed with date palm profilin (Pho d 2) and peach LTP (Pru p 3), and specific IgE (sIgE) to Phl p 12 and Pru p 3 was analyzed using single FEIA and microarray. Results: Fifteen of 201 patients with negative results for LTP in the SPT were sensitized to this allergen in the in vitro tests, and 18 of 41 patients with positive results for LTP in the SPT were not sensitized according to the in vitro tests. Seventeen of 186 patients with negative results for profilin in the SPT were sensitized to Phl p 12 by serum sIgE, and 30 out of 56 patients with positive results for profilin in SPT were not sensitized to Phl p 12 according to the other tests. Moderate agreement was observed between the 3 techniques studied. Conclusions: SPT is a sensitive technique for detecting sensitization to LTP and profilin. Its results are similar to those of in vitro techniques, especially in patients with negative SPT results for peach LTP and palm tree profiling (AU)


Assuntos
Feminino , Humanos , Masculino , Testes Cutâneos , Técnicas In Vitro/métodos , Técnicas In Vitro , Hipersensibilidade/diagnóstico , Profilinas/análise , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Alimentar/imunologia , Proteínas Ligadas a Lipídeos/imunologia , Estudos Retrospectivos , Imunoensaio/métodos , Alérgenos , Técnicas Imunológicas/métodos , Grupos Controle , Hipersensibilidade Imediata/imunologia
8.
Allergol. immunopatol ; 42(5): 387-394, sept.-oct. 2014. tab
Artigo em Inglês | IBECS | ID: ibc-127270

RESUMO

Background: Sensitisation to pan-allergens has become an interesting tool for the study of the allergenic profile of different populations. Profilins are one of the most common pan-allergens to be studied because they are responsible for a large number of sensitisations and are clearly related to cross-reactivity and co-sensitisation. Objectives: The objective of this study was to investigate the profile of sensitisation to profilins and to correlate it with sensitisation to foods and pollens. Methods: Six hundred and fifty-four consecutive patients were skin-prick tested with a battery of common allergens including pollens, epithelia, mites and moulds and profilin and divided into three groups depending on their sensitisation profile (non-atopic, atopic with pollinosis and atopic without pollinosis). Patients with symptoms were challenged and diagnosed with the offending food extracts. Profilin sensitisation was identified and analysed in detail. Results: According to the classification of the population, the prevalence of profilin sensitisation was estimated at 2.9% in patients suffering respiratory allergy, 4.2% in atopic patients, and 5.9% in pollen-sensitised individuals. Positive association was observed between pollen (except Cupressus and olive) and profilin but not with moulds, mites or epithelia. With respect to foods, positive association was only observed between profilin and melon sensitisation. Lastly, in terms of symptoms, positive association was only observed between profilin sensitisation and OAS. Conclusion: Profilin sensitisation seems to be a marker of pollen-related poly-sensitisation in our area. Pan-allergen diagnosis seems to be an essential tool for developing and improving selection of the correct treatment for allergic patients (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Profilinas/análise , Profilinas , Profilinas/imunologia , Hipersensibilidade/diagnóstico , Hipersensibilidade/imunologia , Alérgenos/análise , Alérgenos , Pele/enzimologia , Pele/imunologia
10.
J. investig. allergol. clin. immunol ; 23(5): 345-350, ago. 2013. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-114947

RESUMO

Antecedentes y objetivo: El polen de Humulus japonicus constituye la causa mayor de polinosis en Corea. La profilina (15 kDa) de H. scandens ha mostrado ser fuertemente alergénica en los pacientes chinos. También la profilina se ha detectado en extractos de polen del H. japonicus mediante análisis de proteómica e inmunoblotting. Sin embargo la alergenicidad de los alérgenos aislados no ha sido investigada en los pacientes de Corea. En este estudio, se produce la profilina recombinante del polen de H. japonicus y se evalúa su alergenicidad. Métodos: Las secuencias de cDNA que codifican dos isoalérgenos fueron clonadas mediante RT-PCR y las proteína recombinantes se expresaron en Escherichia coli. Las reactividades de IgE para los alérgenos recombinantes fueron analizadas mediante ELISA. Resultados: Se comprobó que las secuencias de aminoácidos deducidas de la profilina de H. japonicus mostraban una homología del 68.7-80.2% con las profilinas de Artemisia (Art v 4), Ambrosia (Amb a 14) y abedul (Bet v 2). Los dos isoalérgenos de la profilina de H. japonicus mostraron una identidad del 78.2%. Las secuencias de cDNA de estos dos isoalérgenos mostraron una identidad del 98.5 (AY268422) y 98.7% (AY268424) con los de H. scandens. Muestras de suero de los pacientes sensibilizados a H. japonicus mostraron una reactividad de IgE en el 12.9% de los casos. La reactividad de IgE frente a ambos isoalérgenos recombinantes de la profilina de H. japonicus indica que dicha profilina puede no ser un componente alergénicamente dominante en este tipo de polinosis. Las profilinas recombinantes mostraron una leve inhibición del 0-9.3% sobre el extracto crudo de polen de H. japonicus. Conclusiones: Los dos isoalérgenos aislados de la profilina de H. japonicus están altamente conservados y muestran homología con la de Artemisia, Ambrosía y abedul, y juegan un papel menor en la polinosis más frecuente de Corea (AU)


Background and objective: Pollen from Japanese hop, Humulus japonicus, is a major cause of pollinosis in Korea. Profilin (15 kDa) from Humulus scandens has been associated with strong allergenicity in allergic Chinese patients. Profilin has also been detected in pollen extract from Korean Japanese hop by proteomic analysis and immunoglobulin (Ig) E immunoblotting. However, the allergenicity of allergens isolated from Japanese hop has not been investigated in Korean individuals. This study was undertaken to produce recombinant profilin from Japanese hop and evaluate its allergenicity. Methods: Complementary DNA sequences encoding 2 isoallergens were cloned by reverse transcription polymerase chain reaction and their recombinant proteins expressed in Escherichia coli. The IgE-binding reactivities of the recombinant allergens were assessed by enzymelinked immunosorbent assay. Results: The deduced amino acid sequences of the H japonicus profilins were 68.7% to 80.2% homologous with profilins from mugwort (Art v 4), ragweed (Amb a 14), and birch (Bet v 2). Two isoallergens of profilin from H japonicus were 78.2% identical. Notably, the cDNA sequences of these 2 isoallergens were 98.5% (AY268422) and 98.7% (AY268424) identical to those of H scandens. Serum samples from Japanese hop–sensitized individuals showed 12.9% IgE reactivity to both of the recombinant profilin isoallergens from H japonicus, indicating that profilin may not be an allergenically dominant component of Japanese hop pollen. The recombinant profilins showed only 0% to 9.3% inhibition of the crude extract. Conclusions: Two isoallergens of profilin that are highly conserved with those of mugwort, ragweed, and birch were identified in H japonicus. Profilins from Japanese hop pollen may play a minor role in the pathogenesis of pollinosis in Koreans (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Profilinas , Profilinas/imunologia , Lupulus humulus/efeitos adversos , Lupulus humulus/isolamento & purificação , Rinite Alérgica Sazonal/diagnóstico , Rinite Alérgica Sazonal/imunologia , Rinite Alérgica Sazonal/complicações , Western Blotting/métodos , Western Blotting/normas , Western Blotting , Ensaio de Imunoadsorção Enzimática/instrumentação , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática , Rinite Alérgica Sazonal/epidemiologia , Rinite Alérgica Sazonal/prevenção & controle
13.
Allergol. immunopatol ; 39(3): 145-149, mayo-jun. 2011. tab, graf
Artigo em Inglês | IBECS | ID: ibc-90102

RESUMO

Background: Date palm pollen allergy is frequently associated with polysensitisation. Observational studies have suggested that date-palm-sensitised individuals could be included in a distinct group of polysensitised patients. The objectives of the study were to analyse the clinical characteristics of a group of patients diagnosed of date-palm pollen allergy and to compare them with pollen allergic patients without date-palm sensitisation. Methods: Forty-eight palm-pollen sensitised individuals were classified as Group A. A control group of 48 patients sensitised to pollens but without palm-pollen allergy were included as Group B. All individuals were skin prick tested with a common battery of aeroallergens. Information about age, sex, family history of atopy, respiratory symptoms, food allergy and sensitisation to other pollens were considered variables of the study. Specific IgE and the allergogram todate-palm pollen were determined in a subgroup of Group A. Results: Significant differences in the family history of atopy and number of sensitisations were observed. Both parameters were significantly higher in Group A. Group A showed high prevalence of asthma and higher level of sensitisation to foods (p < 0.05). Significant differences were obtained for sensitisation to epithelia and pollens. Pho d 2 was the most commonly recognised allergen (83.3%) in the palm-pollen allergic group. Conclusions: Date-palm pollen allergic patients constitute a homogeneous group characterised for showing bronchial asthma, sensitisation to food allergens and polysensitisation. These results suggest that the reasons for sensitisation to date-palm pollen remain to be elucidated, but could relate to the existence of as yet non-identified pan-allergens (AU)


Assuntos
Humanos , Masculino , Feminino , Rinite Alérgica Sazonal/complicações , Rinite Alérgica Sazonal/diagnóstico , Região do Mediterrâneo/epidemiologia , Profilinas/efeitos adversos , Profilinas/imunologia , Rinite Alérgica Sazonal/etnologia , Estudos Retrospectivos , Testes Cutâneos/métodos , Imunoglobulina E , Hipersensibilidade Alimentar/etiologia , Hipersensibilidade Alimentar/imunologia
15.
Allergol. immunopatol ; 35(supl.1): 36-40, mayo 2007. ilus
Artigo em Espanhol | IBECS | ID: ibc-144839

RESUMO

La sensibilización múltiple a pólenes es una situación clínica frecuente en España que condiciona la eficacia de la inmunoterapia alérgeno-específica. Hoy sabemos que se necesita optimizar el diagnóstico para seleccionar el tratamiento adecuado para cada paciente. Abordar la alergología a nivel molecular aparentemente complica el manejo asistencial y sin embargo, basta cruzar los registros de una base de datos de familias de proteínas secuenciadas1 con los de otra de alergenos conocidos , para obtener un número limitado de proteínas alergénicas que a su vez pueden ser clasificadas por su origen taxonómico3. La información final puede consultarse de forma integrada en otra base de datos4 que actualiza ágilmente lo referente a características bioquímicas, origen, función biológica, documentación clínica y disponibilidad industrial de alergenos. Todos los alergenos polínicos pertenecen a 29 familias de proteínas, siendo las más abundantes las expansinas, profilinas y polcalcinas. La naturaleza ubicua de proteínas como las profilinas y polcalcinas les confieren carácter de panalergenos y explica la reactividad cruzada, que es interpretada erróneamente por los clínicos como multisensibilidad. Otras familias de alergenos, como las proteínas transportadoras de calcio (LTPs), son más restringidas pero se asocian a un perfil de especial gravedad clínica, lo que resulta especialmente útil a la hora de indicar la inmunoterapia. El diagnóstico más preciso lo dan las proteínas muy selectivas de especie, como las expansinas de las gramíneas. Aunque es posible la fabricación de alergenos recombinantes para el diagnóstico in vitro, la legislación actual sólo permite el uso de proteínas naturales para inmunoterapia. Sin embargo, es posible aplicar la misma tecnología al estudio de los extractos para vacunas, y parece que la cuantificación de alergenos por parte de los fabricantes es un camino sin retroceso que los clínicos estamos obligados a seguir (AU)


No disponible


Assuntos
Humanos , Dessensibilização Imunológica/métodos , Pólen/efeitos adversos , Rinite Alérgica Sazonal/terapia , Reações Cruzadas/imunologia , Profilinas/imunologia , Testes de Provocação Brônquica , Hipersensibilidade Respiratória/imunologia
16.
Allergol. immunopatol ; 30(2): 62-69, mar. 2002. graf
Artigo em Inglês | IBECS | ID: ibc-134815

RESUMO

Background: allergic disease caused by Parietaria judaica (Pj) has been widely documented in Mediterranean area. Profilins have been identified as widely distributed allergenic proteins. The role of Pj profilin in specific immune response in Pj-sensitized patients is unknown. Methods: skin prick test and determination of specific and total IgE levels in serum were performed in all patients (n = 28) and non-allergic controls (n = 18). Peripheral blood mononuclear cells (PBMC) were isolated from both groups and stimulated with crude extract or highly purified Pj profilin. The production of type I and type II cytokines was determined by specific and polyclonal stimuli in patients and controls. T-cell lines specific to Pj profilin were established and cross-reactivity with another highly purified profilin from Phleum pratense (Phl p) was evaluated. Results: Pj profilin-sensitized patients showed a small but significantly increased in T-cell proliferative response to this profilin compared with non-atopic controls. The production of interleukin (IL)-4 and interferon (IFN)-γ in response to the specific stimulus was undetectable. However, the production of IL-4 in response to a polyclonal stimulus [phytohemagglutinin (PHA)] was significantly higher in atopic patients than in controls. The T-cell response did not correlate with the magnitude of response to skin prick tests with Pj profilin or with Pj-specific serum IgE levels. In addition, the production of IL-4 in response to a polyclonal stimulus (PHA) did not correlate with the individual skin prick tests to Pj profilin or with Pj-specific IgE levels in serum. The T-cell lines tested showed no cross-reactivity with Phl p profilin. Conclusions: our results suggest that Pj profilin is partly responsible for the T-cell-mediated response in patients allergic to Pj. The high skin reactivity to Pj profilin is these patients was accompanied by a small increase in the T-cell response to this profilin. The response was highly specific since Pj profilin specific T-cell lines showed no cross-reactivity with a highly homologous profilin from Phl p. The lack of correlation between the proliferative T-cell response and polyclonal IL-4 production with allergen-specific serum IgE and skin reactivity probably indicates that some of the responding T-cells may be involved in immune reactions other than those supporting IgE production (AU)


Antecedentes: La enfermedad alérgica originada por Parietaria judaica (Pj) ha sido ampliamente documentada en la zona Mediterránea. Las profilinas han sido identificadas como proteínas alergénicas de amplia distribución. El papel de la profilina de Pj en la respuesta inmunológica específica en pacientes sensibilizados a la Pj es desconocida. Métodos: Se determinaron en todos los pacientes (n = 28) y controles normales no atópicos (n = 18) el test de reactividad cutánea y los niveles tanto de IgE específicas como totales en suero. Se aislaron células mononucleares de sangre periféica (PBMC) en ambos grupos y fueron estimuladas con el extracto crudo o con la profilina altamente purificada de Pj. Se determinaron también la producción de citocinas de tipo I y de tipo II, con estimulaciones específicas o policlonales en pacientes y controles. Establecimos líneas de células T específicas por la profilina de Pj y evaluamos la reactividad cruzada con otra profilina altamente purificada de Phleum pratense (Phl p). Resultados: Los pacientes sensibilizados a la profilina de Pj muestran un ligero pero significativo incremento en la respuesta proliferativa T frente la profilina respecto con el grupo de controles no alérgicos. La producción de IL-4 y IFN-γ en respuesta a estímulos específicos fue indetectable. Sin embargo, la producción de IL-4 en respuesta a un estímulo policlonal (PHA), fue significativamente mayor en pacientes atópicos que en los controles no alérgicos. La respuesta celular T no se corresponde ni con la magnitud de la repuesta en la reactividad cutánea a la profilina de Pj ni con los niveles de IgE en suero específicos para la Pj. La producción de IL-4 frente a un estímulo policlonals (PHA), no se correlaciona con la reactividade cutánea a la profilina ni con los niveles de IgE en suero. Las líneas específicas T testadas no mostraron ninguna reactividad cruzada con la profilina de (Phl p). Conclusiones: Nuestros resultados sugieren que la profilina de Pj es responsable en parte de la respuesta mediada por células T en pacientes alérgicos a la Pj. La elevada reactividad cutánea de estos pacientes, se acompaña de un pequeño incremento en la respuesta de células T a esta profilina. La respuesta es muy específica porque las líneas de células T no presentaron respuesta cruzada con la profilina altamente homóloga de Phl p. La falta de correlación entre la respuesta proliferativa de las células T y la producción policlonal de IL-4 con los niveles de IgE específicos en suero y la reactividad cutánea probablemente indica que algunas de las células T respondedoras estarían involucradas en una reacción inmunitaria diferente a la que promueve la producción de IgE (AU)


Assuntos
Humanos , Parietaria/efeitos adversos , Hipersensibilidade Alimentar/imunologia , Imunoglobulina E/análise , Hipersensibilidade Imediata/imunologia , Antígenos de Plantas/efeitos adversos , Profilinas/imunologia , Testes de Irritação da Pele , Proteínas na Dieta/efeitos adversos , Estudos de Casos e Controles
17.
Allergol. immunopatol ; 29(1): 9-15, ene. 2001. graf
Artigo em Inglês | IBECS | ID: ibc-150535

RESUMO

Background: Phleum pratense (Phl p) pollen is a known cause of allergic disease worldwide. Profilins have been identified as functional plant pan-allergens. The role of Phl p profilin in the specific immune response in sensitized Phl p patients is unknown. Methods: skin prick test and specific serum IgE levels were performed in 26 patients allergic to Phl p and in 18 nonallergic control donors. Peripheral blood mononuclear cells were isolated from both groups and stimulated with crude extract or highly purified Phl p profilin, and the production of type I and type ll cytokines was determined in patients and controls stimulated with specific and polyclonal stimulus. T-cell lines specific to Phl p profilin were established from PBMCs and cross-reactivity with another highly purified profilin from Parietaria judaica (Pj) was evaluated. Results: patients allergic to Phl p profilin showed increased T-cell-proliferative responses to this profilin compared with control subjects. The production of IL-4 and IFN-g in response to the specific stimulus was undetectable. However, the production of IL-4 and IFN-g in response to a polyclonal stimulus (PHA) was measurable and different for atopic patients and control subjects: IL-4 was higher (p < 0.001) in allergic patients and IFN-g lower (although not significant) in controls. Neither the T-cell responses nor the production of IL-4 in response to a polyclonal stimulus (PHA) correlated with the individual degree of cutaneous response to Phl p profilin or to the levels of specific Phl p IgE. The T-cell lines tested did not show any cross-reactivity with Pj profilin. Conclusions: Phl p profilin is in part responsible for the T-cell mediated immunological response in patients allergic to Phl p. The response is very specific since Phl p profilin specific T-cell lines did not show cross-reactivity with a highly homologous profilin from Parietaria judaica (Pj). The lack of correlation between the proliferative T-cell response and polyclonal IL-4 production with allergen-specific serum IgE and SPT probably indicates that some of the responding T-cells may be involved in immune reactions other than the support of IgE (AU)


Antecedentes: el polen de Phleum pratense (Phl p) es una de las causas más frecuentes de alergia respiratoria en el mundo occidental. Las profilinas han sido identificadas como proteínas pan-alergénicas con una amplia distribución en el reino vegetal. El papel de la profilina de Phl p en la respuesta inmunológica específica en pacientes sensibilizados al Phl p es desconocida. Métodos: se practicaron a 26 pacientes alérgicos a Phl p y a 18 controles no atópicos pruebas cutáneas cuantificadas a profilina de Phl p, así como determinación de IgE específica a polen de Phl p. Se aislaron células mononucleares de sangre periférica (PBMC) en ambos grupos y tras estímulo con el extracto crudo o con la profilina altamente purificada de Phl p, se determinó la producción de citocinas de tipo I y de tipo II, por medio de estimulaciones específicas o policlonales tanto en pacientes como en controles. Se establecieron líneas de células T específicas por la profilina de Phl p y se evaluó la posible reactividad cruzada con otra profilina altamente purificada de Parietaria judaica (Pj). Resultados: los pacientes sensibilizados a la profilina de Phl p mostraron un incremento en la respuesta proliferativa T frente la profilina respecto al grupo de controles no alérgicos. La producción de IL-4 e IFN-g en respuesta al estímulo específico fue indetectable. Sin embargo, la producción de IL-4 en respuesta a un estímulo policlonal (PHA) fue significativamente mayor en pacientes alérgicos que en los controles y la producción de IFN-Υ fue menor en el grupo de alérgicos que en los controles. Ni la respuesta celular T ni la producción de IL-4 frente a un estímulo policlonal se corresponden con la magnitud de la respuesta en la reactividad cutánea a la profilina de Phl p, o con los niveles de IgE específica a Phl p en suero. Las líneas específicas T testadas no mostraron ninguna reactividad cruzada con la profilina de Pj.Conclusiones: nuestros resultados sugieren que la profilina de Phl p es responsable en parte de la respuesta mediada por células T en pacientes alérgicos a Phl p. La respuesta es muy específica porque las líneas de células T no presentaron respuesta cruzada con la profilina altamente homóloga de Pj. La falta de correlación entre la respuesta proliferativa de las células T y la producción policlonal de IL-4 con los niveles de IgE específicos en suero y la reactividad cutánea probablemente indica que algunas de las células T respondedoras podrían estar involucradas en reacciones inmunitarias distintas de las que promueven la producción de IgE (AU)


Assuntos
Humanos , Adulto , Alérgenos/imunologia , Hipersensibilidade Imediata/imunologia , Imunoglobulina E/imunologia , Proteínas dos Microfilamentos/imunologia , Proteínas de Vegetais Comestíveis/imunologia , Pólen/imunologia , Rinite Alérgica Sazonal/imunologia , Subpopulações de Linfócitos T/imunologia , Linhagem Celular , Reações Cruzadas , Hipersensibilidade Imediata/etiologia , Interferon gama/biossíntese , Interleucina-4/biossíntese , Ativação Linfocitária , Profilinas , Rinite Alérgica Sazonal/etiologia , Testes Cutâneos , Subpopulações de Linfócitos T/metabolismo
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