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1.
Med. oral patol. oral cir. bucal (Internet) ; 24(4): e537-e544, jul. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-185668

RESUMO

Background: To determine whether saliva is a good means of evaluating concentrations of oxidative stress bio-markers, analyzing the correlation between concentrations in saliva and in follicular tissue, and to compare bio-marker concentrations in patients with one asymptomatic mandibular impacted third molar (MITM) (before ex-traction) with a healthy control, and to determine how biomarkers are modified by extraction. Material and Methods: 80 patients with one asymptomatic MITM and 80 healthy controls were included. Saliva samples were collected from all subjects (before extraction in the study group) to evaluate Myeloperoxidase (MPO) and Malondialdehyde (MDA) concentrations. Follicular tissues were obtained during surgery to measure biomarkers. One month after extraction, saliva samples were collected to assess changes of oxidative stress. Results: Salivary MPO and MDA showed positive correlation with concentrations in follicular tissue (MPO: correlation coefficient = 0.72, p = 0.025; MDA: = 0.92, p = 0.001). Patients with asymptomatic MITMs showed higher salivary concentrations of oxidative stress biomarkers than healthy control subjects, with statistical significance for both MPO (p < 0.001) and MDA (p < 0.001). One month after extraction, salivary biomarkers decreased significantly in the study group (p < 0.001)


No disponible


Assuntos
Humanos , Peroxidase , Dente Impactado , Biomarcadores , Malondialdeído , Dente Serotino , Saliva
2.
Int. microbiol ; 22(2): 203-215, jun. 2019. graf, tab
Artigo em Inglês | IBECS | ID: ibc-184827

RESUMO

The enzymatic and non-enzymatic antioxidant activities of a solid-state fermentation system (SSFS) employing six basidiomycete and four ascomycete fungi on orange peel have been evaluated. Class comparisons revealed highly significant effect of fungal group on the antioxidant activity. Peroxidase activity appeared only in the basidiomycete fungi (particularly Pleurotus columbinus, Ganoderma resinaceum, and Pleurotus floridanus) whereas catalase activity appeared in the two fungal groups in favor of the ascomycetes (particularly Paecilomyces variotii and Aspergillus fumigatus). Maximal peroxidase and minimal catalase activities were found at moderate phenolic content, with extreme phenolic levels leading to low peroxidase activity but high catalase activity. Production of the non-enzymatic antioxidants (phenolics, flavonoids, reducing power, and DPPH scavenging) was in favor of the ascomycetes, which showed great native ability to synthesize flavonoids and also to release flavonoids from orange peel. The basidiomycete fungi, which have limited native ability to produce phenolics, had high ability to consume orange peel phenolics. By contrast, the ascomycete fungi exhibited great native ability for production of phenolics and low ability to consume exogenous phenolics


No disponible


Assuntos
Antioxidantes/análise , Ascomicetos/crescimento & desenvolvimento , Basidiomycota/crescimento & desenvolvimento , Reatores Biológicos/microbiologia , Peroxidase/análise , Meios de Cultura/química , Ascomicetos/metabolismo , Basidiomycota/metabolismo , Catalase/análise , Fermentação
3.
Rev. esp. cardiol. (Ed. impr.) ; 72(4): 317-323, abr. 2019. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-187897

RESUMO

Introducción y objetivos: En el infarto agudo de miocardio con elevación del segmento ST, el ADN libre circulante podría originarse de los leucocitos activados en la lesión coronaria. El objetivo fue investigar la relación entre el ADN libre y la reperfusión coronaria. Métodos: Se incluyó a 116 pacientes, tratados con angioplastia primaria y tromboaspiración. Se cuantificó el ADN libre coronario (durante la aspiración) y periférico (al final del procedimiento), así como la troponina T ultrasensible y la mieloperoxidasa. El objetivo primario fue la no resolución del segmento ST (RST) (≥ 70%) y el secundario la ausencia de flujo Thrombolysis In Myocardial Infarction 3 (TIMI 3) final. Resultados: Se obtuvo RST en 51 (44%) pacientes y flujo TIMI 3 en 97 (84%). Los pacientes sin RST y flujo TIMI 3 tuvieron un menor gradiente ADN libre periférico-coronario (p = 0,02 y p = 0,04, respectivamente). Un gradiente pequeño de ADN libre (< 1,82 ng/ml) se asoció a una mayor frecuencia de no RST (65 frente al 30%; p = 0,001) y de falta de flujo TIMI 3 (21 frente al 3%; p = 0,05. Tras el ajuste multivariable, un gradiente de ADN libre pequeño fue predictivo de no RST (OR = 4,50; IC95%, 1,60-12,62; p = 0,004), en tanto que hubo una tendencia no significativa para el flujo TIMI 3 (p = 0,14). El ADN libre no se correlacionó con la troponina o la mieloperoxidasa. Conclusiones: Un gradiente pequeño de ADN libre periférico-coronario, como expresión de una alta carga de ADN libre coronario, se asocia con no RST en el infarto agudo de miocardio. El ADN libre coronario podría reflejar la activación de los neutrófilos. La potencial contribución de este fenómeno al fracaso de la tromboaspiración requiere nuevos estudios


Introduction and objectives: Cell-free DNA (cfDNA) in ST-segment elevation myocardial infarction might originate from hyperactivated leukocytes at the coronary lesion. Our aim was to investigate the relationship between cfDNA and coronary reperfusion. Methods: We studied 116 patients treated with primary angioplasty using thrombus aspiration. Coronary (during aspiration) and peripheral (at the end of the procedure) blood samples were drawn for cfDNA, as well as high-sensitivity troponin T and myeloperoxidase quantification. The primary endpoint was no ST-segment resolution (STR) (≥ 70%) and the secondary endpoint was lack of final Thrombolysis In Myocardial Infarction flow 3 (TIMI 3). Results: ST-segment resolution was achieved in 51 (44%) patients and TIMI 3 flow in 97 (84%). Patients without STR and TIMI 3 flow had a smaller peripheral-coronary cfDNA gradient (P = .02 and P = .04 respectively). A small cfDNA gradient (< 1.82 ng/mL) was associated with a higher rate of no STR (65% vs 30%; P = .001) and lack of TIMI 3 flow (21% vs 3%; P = .05). After multivariable adjustment, the small cfDNA gradient was predictive of no STR (OR, 4.50; 95%CI, 1.60-12.62; P = .004), while there was a nonsignificant trend for final TIMI 3 flow (P = .14). Cell-free DNA levels did not correlate with troponin T or myeloperoxidase. Conclusions: A small peripheral-coronary cfDNA gradient, as an expression of high coronary cfDNA burden, is associated with no STR in acute myocardial infarction. Intracoronary cfDNA might reflect neutrophil activation. Whether this phenomenon contributes to thrombus aspiration failure requires further study


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Infarto do Miocárdio com Supradesnível do Segmento ST/cirurgia , Intervenção Coronária Percutânea/métodos , Ácidos Nucleicos Livres/análise , Angioplastia/métodos , Reperfusão Miocárdica/métodos , Trombectomia/métodos , Troponina/análise , Peroxidase/análise , Estudos Prospectivos
4.
Rev. int. androl. (Internet) ; 16(3): 87-94, jul.-sept. 2018. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-178033

RESUMO

It has been reported in the literature that proinflammatory interleukin-1 beta (IL-1β) is increased in cases of testicular ischemia reperfusion (I/R) damage. This information suggests that anakinra, an IL-1β antagonist, may be effective in testicular I/R damage. Objective: In our study, we investigated the effect of anakinra on testicular I/R damage induced in rats with torsion/detorsion. Methods: The 50mg/kg anakinra+testicular torsion/detorsion (KTD-50) and 100mg/kg anakinra+testicular torsion/detorsion (KTD-100) groups received an intraperitoneal (i.p.) injection of 50mg/kg and 100mg/kg of anakinra, respectively. In turn, the testicular torsion/detorsion (TTD) and sham operation (SOG) groups received a single dose of distilled water as a solvent 1h before ketamine anaesthesia. After the testes of the TTD, KTD-50 and KTD-100 groups were subjected to torsion and detorsion for 4h each, the rats were killed with a high-dose anaesthesia, and their testicles were removed and evaluated through biochemical, gene expression and histopathological examinations. The results were evaluated in comparison with those of the SOG group. Results: The levels of malondialdehyde (MDA), myeloperoxidase (MPO) and IL-1β showed significant increases in the TTD group, which underwent torsion/detorsion, compared to the KTD-50, KTD-100 and SOG groups. Conversely, the levels of glutathione (tGSH), glutathione peroxidase (GPO) and glutathione s-transferase (GST) were found to be significantly higher in the KTD-50, KTD-100 and SOG groups than in the TTD group. Conclusion: Anakinra at a 100mg/kg dose histologically suppressed better oxidative stress and tunica albuginea, germ cell, seminiferous tubule and interstitial damage in the testicular tissue compared to a 50mg/kg dose. Experimental results indicate that anakinra might be beneficial in the attenuation of testicular I/R damage


Antecedentes: Se ha reportado en la literatura que citoquinas interleuquina-1 beta (IL-1β) es mayor en el daño de la isquemia reperfusión testicular (I/R). Esta información sugiere que la anakinra, que es un antagonista IL-1β puede ser eficaz en daño testicular I/R. Objetivo: En nuestro estudio se investigó el efecto de este medicamento en daño testicular I/R inducida en ratas con detorsion/torsión. Métodos: KTD-50 grupo recibido intraperitonealmente (i.p.) inyección de 50mg/kg y KTD-100 Grupo 100mg/kg de anakinra, mientras TTD (control) y SOG (sham grupo operación) recibieron una dosis única de agua destilada como solvente, una hora antes de ketamina anestesia. Después de que los testículos de TTD, KTD-50 y KTD-100 grupos fueron sometidas a torsión y detorsion para cuatro por cuatro horas, las ratas fueron asesinados con altas dosis de anestesia, sus testículos fueron extraídos y evaluados a través de la expresión génica, bioquímicas e histopatológicas de exámenes. Los resultados fueron evaluado en comparación con la de SCG grupo. Resultados: Los niveles de MDA, MPO y IL- 1β mostraron incrementos significativos en el grupo TTD/torsión detorsion administrados frente a-50, KTD KTD-100 y SOG grupos. Por el contrario, los niveles de tGSH, GPO y GST resultaron significativamente más altas en KTD-50 KTD-100 y grupos SOG de TTD en grupo. Conclusión: La anakinra en 100mg/kg dosis mejor histológicamente suprime el estrés oxidativo y la túnica albuginea, células germinales, túbulos seminíferos apretadamente enrollados intersticial y daño en el tejido testicular en comparación con la dosis de 50mg/kg. Los resultados experimentales indican que la anakinra puede ser beneficiosa en la atenuación de los daños I/R testicular


Assuntos
Animais , Ratos , Interleucina-1beta/antagonistas & inibidores , Traumatismo por Reperfusão , Estresse Oxidativo , Doenças Testiculares/tratamento farmacológico , Modelos Animais de Doenças , Peroxidase/fisiologia , Glutationa/análise , Glutationa Peroxidase/fisiologia , Expressão Gênica/fisiologia
6.
Rev. esp. patol. torac ; 29(3): 155-166, oct. 2017. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-167912

RESUMO

Objetivos: establecer un modelo murino de fibrosis pulmonar inducida por bleomicina, investigando el posible papel protector del sistema endocannabinoide (SE) frente a la fibrosis. Métodos: se emplearon ratones salvajes (C5BL/6 y Balb/c) así como la cepa TRPV1-/-. Tras una única dosis intratraqueal de bleomicina, se analizó la respuesta fibrótica mediante un análisis histológico, la determinación de la expresión de marcadores del proceso profibrótico, el estudio de la actividad mieloperoxidasa y del contenido en hidroxiprolina del pulmón, así como el análisis de la expresión génica de VIP, PACAP, IL-1β, IL-6, TNF-α e IL-11, y el estado de activación de las rutas MAPKs (fosfo-JNK, fosfo-ERK) de la ruta de NF-κB (p-IκBα), la ruta de β-catenina y del TGFβ (GSK-3B), la activación de SMAD (pSMAD2) y pSTAT3, a nivel proteico. Resultados: la fibrosis pulmonar inducida por bleomicina en los ratones de la cepa TRPV- /- fue más severa que en la cepa salvaje C5BL/6. El contenido en hidroxiprolina y la actividad mieloperoxidasa fue mayor en los ratones TRPV1-/-. Se detectó un incremento significativo en la expresión génica de citoquinas proinflamatorias (TNF-a, IL-1b, IL11 e IL-6), pero no de VIP o PACAP, en la cepa TRPV1-/-. A nivel proteico, la expresión de pIKBα, pSTAT3, pSMAD2 y pJNK, pero no la de pERK, se vio incrementada en los ratones TRPV1-/-. Conclusiones: el modelo murino de fibrosis pulmonar inducida por bleomicina sigue siendo clave para continuar profundizando los conocimientos acerca de la patogénesis de la FPI. La modulación del SE podría tener un papel protector frente a la fibrosis pulmonar


Objectives: to establish a murine model of bleomycin-induced pulmonary fibrosis, analysing the possible protective role of the Endocannabinoid System (ES) against fibrosis. Methods: wild C5BL/6 and Balb/c mice, as well as the genetically modified strain TRPV1- /- were used. After a single dose of intratracheal bleomycin, the fibrotic response was analysed though histologic studies, the assessment of proinflammatory markers, myeloperoxidase activity, hydroxyproline content, genetic expression of VIP, PACAP, IL-1 β, IL-6, TNF-α and IL-11, as well as MAPK route (phospho-JNK, phospho-ERK), NF-κB (p-IκBα), β-cathenin, TGF-β (GSK-3B), SMAD (p-SMAD2) and pSTAT3, at a protein level. Results: pulmonary fibrosis was more severe in TRPV1-/- mice compared to C5BL/6 mice. A significant increase in proinflammatory markers such as TNF-α, IL-1β, IL11 and IL-6, but not VIP or PACAP, was observed. pIKBα, pSTAT3, pSMAD2 and pJNK, but not pERK, were increased at a protein level in TRPV-/- mice. Conclusions: the murine model of bleomycin-induced lung fibrosis remains a keystone to pioneer current investigation in lung fibrosis. Modulation of the ES might have a protective role


Assuntos
Animais , Camundongos , Fibrose Pulmonar/induzido quimicamente , Fibrose Pulmonar/veterinária , Bleomicina/uso terapêutico , Peroxidase/uso terapêutico , Endocanabinoides/uso terapêutico , Expressão Gênica , Modelos Animais , Laparotomia/métodos , Laparotomia/veterinária , Imuno-Histoquímica/métodos , Western Blotting/métodos
7.
J. physiol. biochem ; 72(3): 405-419, sept. 2016. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-168284

RESUMO

A lot of evidence for the importance of CD26/dipeptidyl peptidase IV (CD26/DPP IV) in immunoactivation has been reported; however, its involvement in colitis remains unclear. The aim of this study was to investigate the influence of CD26/DPP IV deficiency on immunophenotypic changes associated with dextran sulfate sodium (DSS)-induced colitis in wild-type (WT) and CD26-deficient mice. Development of clinical symptoms of colitis and animal health status parameters were assessed; the expression of the nuclear factor (NF)-κB p65 subunit was measured by quantitative real-time PCR, while cell characterization was determined by flow cytometry and immunohistochemical staining. DSS treatment induced loss of body weight and colon length shortening in both mouse strains. An increase of myeloperoxidase activity in CD26-deficient mice was more intensive than in WT mice, in spite of similar histopathological changes. Furthermore, a significant increase in the expression of NF-κB p65 subunit in the colon of CD26-deficient mice was determined. The percentage of splenic CD4+ and CD8+ cells in the acute phase of colitis was significantly decreased in WT mice, while in the same period, an increase in the percentage of splenic CD8+ cells was present in CD26-deficient mice. Development of colitis was accompanied by a significant increase in the percentage of intrahepatic NKT cells in both mouse strains, but their percentage in spleen was increased only in CD26-deficient mice. CD26 deficiency was associated with a heightened response to DSS accompanied by increased expression of NF-κB p65 subunit and distinct changes in leukocyte trafficking. These results provide new insights into the role of CD26/DPP IV during the development of colitis (AU)


No disponible


Assuntos
Animais , Masculino , Feminino , Camundongos , Colite Ulcerativa/metabolismo , Colo/metabolismo , Modelos Animais de Doenças , Mucosa Intestinal/metabolismo , Infiltração de Neutrófilos , Dipeptidil Peptidase 4/metabolismo , Progressão da Doença , Regulação da Expressão Gênica , Remissão Espontânea , Tamanho do Órgão , Peroxidase/metabolismo , Biomarcadores , Fator de Transcrição RelA , Organismos Livres de Patógenos Específicos
8.
Reumatol. clín. (Barc.) ; 11(1): 17-21, ene.-feb. 2015. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-132358

RESUMO

Objetivo. Determinar la positividad y la correlación clínica de los anticuerpos contra el citoplasma del neutrófilo (ANCA), teniendo en cuenta la interferencia de los anticuerpos antinucleares (ANA). Material y método. Se realizó un estudio prospectivo en el Laboratorio de Inmunología del Centro Nacional de Genética Médica de Cuba durante un año. Se incluyó a 267 pacientes con indicación de ANCA. Las determinaciones de ANCA a diferentes puntos de corte y de ANA se realizaron mediante inmunofluorescencia indirecta. Los anticuerpos antiproteinasa 3 y antimieloperoxidasa fueron determinados mediante ELISA. Resultados. Nuestro estudio mostró que la mayor positividad de ANCA fue vista en pacientes con vasculitis asociadas a ANCA, artritis reumatoidea y lupus eritematoso sistémico. Fue superior la presencia de ANCA sin especificidad por la proteinasa 3 o la mieloperoxidasa en pacientes con ANA y se observó poca relación entre el patrón perinuclear confirmado en formalina y la presencia de anticuerpos frente a la mieloperoxidasa. Los mayores valores de sensibilidad y especificidad para el diagnóstico de las vasculitis se alcanzaron para la determinación de ANCA mediante inmunofluorescencia indirecta a un valor de corte de 1/80 y confirmando la especificidad antigénica mediante ELISA. Conclusiones. Los ANCA pueden estar presentes en un amplio número de enfermedades asociadas a estados inflamatorios y autoinmunes en la población estudiada. Su determinación mediante inmunofluorescencia indirecta seguida de la determinación mediante ELISA tiene gran valor para el diagnóstico de las vasculitis. La determinación de anticuerpos antimieloperoxidasa tiene mayor utilidad que el ensayo en láminas de formalina cuando hay ANA (AU)


Objective. To determine positivity and clinical correlation of anti-neutrophil cytoplasmic antibodies (ANCA), taking into account the interference of antinuclear antibodies (ANA). Material and methods. A prospective study was conducted in the Laboratory of Immunology of the National Cuban Center of Medical Genetic during one year. Two hounded sixty-seven patients with indication for ANCA determination were included. ANCA and ANA determinations with different cut off points and assays were determined by indirect immunofluorescense. Anti proteinase 3 and antimyeloperoxidase antibodies were determined by ELISA. Results. Most positivity for ANCA was seen in patients with ANCA associated, primary small-vessel vasculitides, rheumatoid arthritis and systemic lupus erythematosus. Presence of ANCA without positivity for proteinase 3 and myeloperoxidase was higher in patients with ANA and little relation was observed between the perinuclear pattern confirmed in formalin and specificity by myeloperoxidase. Highest sensibility and specificity values for vasculitides diagnostic were achieved by ANCA determination using indirect immunofluorescense with a cut off 1/80 and confirming antigenic specificities with ELISA. Conclusion. ANCA can be present in a great number of chronic inflammatory or autoimmune disorders in the population studied. This determination using indirect immunofluorescence and following by ELISA had a great value for vasculitis diagnosis. Anti mieloperoxidasa assay has a higher utility than the formalin assay when ANA is present (AU)


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , /metabolismo , Mieloblastina , Peroxidase , Anticorpos Antinucleares , Anticorpos Antinucleares/metabolismo , Sensibilidade e Especificidade , Estudos Prospectivos , Técnica Indireta de Fluorescência para Anticorpo/métodos , Técnica Indireta de Fluorescência para Anticorpo , Vasculite/complicações , Vasculite/diagnóstico , Lúpus Eritematoso Sistêmico/diagnóstico , Artrite Reumatoide/diagnóstico , Hepatite Autoimune/complicações , Hepatite Autoimune/diagnóstico
11.
Actas urol. esp ; 37(2): 79-82, feb. 2013. tab
Artigo em Espanhol | IBECS | ID: ibc-109522

RESUMO

Antecedentes y objetivos: El cáncer de próstata (CaP) es el cáncer más común entre los hombres en la mayoría de las poblaciones occidentales. El gen de polimorfismos de la mieloperoxidasa (MPO) se ha correlacionado con la expresión anormal de la MPO y el aumento del riesgo de varios tipos de cáncer. Nuestro estudio ha tenido como objetivo evaluar la asociación entre los polimorfismos genéticos y el riesgo de cáncer de próstata. Métodos: La genotipificación se realizó mediante el sistema de genotipificación (MassARRAY IPLEX; Sequenom, Inc., San Diego, CA, EE. UU.) en 1.108 pacientes con CaP y 1.525 controles sin cáncer en una población china Han. Resultados: Aunque un PNS (rs8082134, p <0,050) fue significativo, es muy poco común e inestable. Otros PNS no tuvieron diferencias significativas entre las distribuciones de los genotipos en los pacientes con CaP y el grupo control. En total, los PNS en el gen de la MPO no se asocian con el riesgo de CaP. Conclusión: Nuestros datos mostraron una asociación limitada entre los PNS de la MPO y la susceptibilidad al CaP en la población china Han. La posible asociación de rs8082134 de MPO con el riesgo de CaP necesita más aclaraciones (AU)


Background and objectives: Prostate cancer (PCa) is the most common cancer among men in most western populations. The polymorphisms of the myeloperoxidase (MPO) gene have been correlated with abnormal MPO expression and increased risk of various types of cancers. Our study aimed to evaluate the association between the genetic polymorphisms and the risk of prostate cancer. Methods: Genotyping was carried out by using the genotyping system (MassARRAY iPLEX; Sequenom, Inc., San Diego, CA, USA) on 1,108 PCa patients and 1,525 cancer-free controls in a Chinese Han population. Results: Although one SNP (rs8082134, P < 0.050) was significant, it is very rare and unstable. Other SNPs had no significant difference between genotype distributions in the PCa patients and the control group. Totally, SNPs in the MPO gene is not associated with PCa risk. Conclusion: Our data showed a limited association between the MPO SNPs and the susceptibility to PCa in population of Chinese Han population. The possible association of rs8082134 of MPO with PCa risk need further clarification (AU)


Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Peroxidase , Neoplasias da Próstata/diagnóstico , Teste de Histocompatibilidade/métodos , Genótipo , Técnicas de Genotipagem/instrumentação , Técnicas de Genotipagem/métodos , Próstata , Próstata/patologia , Neoplasias da Próstata , Grupos Controle
12.
Reumatol. clín. (Barc.) ; 7(supl.3): s18-s21, dic. 2011. tab
Artigo em Espanhol | IBECS | ID: ibc-147312

RESUMO

Una de las características principales de las vasculitis asociadas a ANCA (VAA) es la ausencia de depósito de complejos inmunes en las biopsias de los tejidos afectados y de consumo de complemento. Sin embargo, en etapas tempranas de enfermedades similares producidas en modelos animales, se ha observado que el sistema del complemento puede participar en la génesis de estas patologías. Varios modelos se han desarrollado en el intento de disecar los mecanismos patogénicos de enfermedades como granulomatosis con poliangitis (Wegener) (GPA) o poliangitis microscópica siendo más exitosos en esta última, sin que hasta el momento se disponga de un modelo satisfactorio para explicar los cambios que llevan a enfermedad granulomatosa vasculítica, máxime si se asocia a anticuerpos contra proteinasa-3 (PR-3), como es el caso en la GPA. Este manuscrito revisa en forma sucinta las evidencias recientes de la presencia de complemento en biopsias de pacientes con VAA, así como modelos animales que ponen de manifiesto la participación del sistema de complemento en su patogenia (AU)


One of the main characteristics of the vasculitis associated with antineutrophil cytoplasm autoantibodies (AASV) is the absence of immune complex deposition in biopsies of affected tissues as well as a lack of complement depletion. However, in early stages of disease induced in animal models, it has been observed that the complement system may be involved in the generation of these diseases. There are various animal models which have been developed with the aim of knowing which are the pathogenic mechanisms in granulomatosis with polyangiitis (Wegener) (GPA) and microscopic polyangiitis (MPA), the latter being explained using these approaches in a more satisfactory manner, as there is lack of a model which reproduces the changes leading to a granulomatous vasculitis associated with antibodies against proteinase-3, as in GPA. This short review presents recent evidence of the presence of complement in biopsies of patients with AASV and the most recent animal models, which show the participation of complement in their etiology (AU)


Assuntos
Humanos , Animais , Camundongos , Proteínas do Sistema Complemento/imunologia , Vasculite Associada a Anticorpo Anticitoplasma de Neutrófilos/imunologia , Anticorpos Anticitoplasma de Neutrófilos/análise , Anticorpos Anticitoplasma de Neutrófilos/imunologia , Anticorpos Monoclonais/uso terapêutico , Autoantígenos/imunologia , Camundongos Endogâmicos C57BL , Peroxidase/imunologia , Mieloblastina/imunologia , Poliangiite Microscópica/imunologia , Rim/imunologia , Rim/patologia , Biópsia , Ativação do Complemento , Complemento C5/antagonistas & inibidores , Complemento C5a/antagonistas & inibidores , Proteínas de Ligação a DNA/deficiência , Granulomatose com Poliangiite/imunologia
13.
Artigo em Inglês | IBECS | ID: ibc-93010

RESUMO

Introduction: As important effector cells of the innate immune system, neutrophils are involved in rejection of solidorgan and/or tissue transplants. But their role in rejection of oral mucosa transplantation (OMT) remains unclear. Theaim of this study is to observe the spatial-temporal change of neutrophils during acute rejection of OMT.Methods: In a rat model of oral mucosal xenotransplantation, myeloperoxidase (MPO), an indicator of influx ofneutrophils was detected by technique of ELISA on day 7 and 30 (D7, D30) of posttransplantation.Results: On D7, MPO level (6.183±0.416, ×102 ng/mg) in the OMT group was significantly higher than in trauma(0.681±0.073, ×102 ng/mg) and normal controls (0.262±0.043, ×102 ng/mg) (P<0.001, respectively), and this levelwas found to correlate with the index of submandibular lymph nodes (ILN), an indicator of inflammation of rejection(r=0.909, P<0.05). Moreover, this level was decreased significantly under FK506 treatment (2.103±0.146,×102 ng/mg, P= 0.005). On D30, MPO in the OMT group (1.063±0.096, ×102 ng/mg) was lower significantly thanthat on D7 (P<0.001), although this level was still higher that of normal controls on D30 (0.532±0.112, ×102 ng/mg, P = 0.042).Conclusion: Neutrophils infiltration was an early event of OMT, which may play important roles on acute rejectionof OMT (AU)


No disponible


Assuntos
Animais , Ratos , Transplante Heterólogo/métodos , Mucosa Bucal/transplante , Infiltração de Neutrófilos/fisiologia , Rejeição de Enxerto/fisiopatologia , Modelos Animais , Peroxidase/análise , Tacrolimo/uso terapêutico
15.
J. physiol. biochem ; 65(4): 339-344, dic. 2009.
Artigo em Inglês | IBECS | ID: ibc-122855

RESUMO

No disponible


Caffeic acid phenethyl ester (CAPE) is a natural product with potent anti-inflammatory, antitumor, and antioxidant activities, and attenuates inflammation and lipid peroxidation. The purpose of the present study was to investigate the effects of CAPE on iron-induced liver damage. Rats were divided into four groups and treated for 7 days with saline (control group), 10 µmol kg CAPE/day s.c. (CAPE group), 50 mg iron-dextran/kg i.p. (IRON group) and CAPE and iron at the same time (IRON+CAPE group). Seven days later, rats were killed and the livers were excised for biochemical analysis. The administration of IRON alone resulted in higher myeloperoxidase (MPO) activity and lipid peroxidation than in the control and CAPE treatment prevented the increase in MPO activity and malondialdeyde (MDA) level. No differences were observed in all four groups with regards to superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities. Our results collectively suggest that CAPE may be an available agent to protect the liver from injury via inhibition of MPO activity (AU)


Assuntos
Animais , Ratos , Insuficiência Hepática/fisiopatologia , Ácidos Cafeicos/farmacocinética , Peroxidase/antagonistas & inibidores , Substâncias Protetoras/farmacocinética , Modelos Animais de Doenças
16.
J. physiol. biochem ; 65(3): 235-241, sept. 2009.
Artigo em Inglês | IBECS | ID: ibc-122868

RESUMO

No disponible


Carbohydrates are thought to function as tags that mark circulatory glycoproteins for rapid clearance. Scavenger endothelial cells (SECs) play the primary role in clearing glycoproteins via receptor-mediated endocytosis in adult animals. We found that horseradish peroxidase (HRP), a glycoprotein, was removed quickly, mostly by receptor mediation from the chicken embryo circulation, but bovine serum albumin was not. The half-life of HRP in the circulation varied with the embryo stage and fell rapidly from 0.73 h at embryonic day 4 (E4) to 0.23 h at E5, with no great difference among stages after E5. HRP clearance was far slower at E3.5 than at E5, but was obviously suppressed by mannan. These results imply that the function of clearing glycoprotein or waste macromolecules from the circulation via receptor-mediated endocytosis appears early in the embryo (AU)


Assuntos
Animais , Embrião de Galinha , Glicoproteínas/metabolismo , Células Endoteliais/fisiologia , Endocitose/fisiologia , Embrião de Galinha/metabolismo , Peroxidase/farmacocinética , Fruturonato Redutase/farmacocinética , Oligossacarídeos/farmacocinética
17.
Rev. lab. clín ; 2(1): 34-46, ene. 2009. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-84590

RESUMO

La cardiopatía isquémica supone el 1,3% de los casos de atención en un servicio de urgencias hospitalario en España. El manejo del paciente es complejo por el riesgo de producir una alta médica incorrecta, el beneficio de instaurar una revascularización rápida y el gasto excesivo por admisiones injustificadas. La última década ha permitido un importante avance en el desarrollo de nuevos marcadores cardíacos. Tradicionalmente los marcadores del síndrome coronario agudo han sido indicadores de necrosis cardíaca. Esta función se ha ampliado actualmente. Aún hay muchas limitaciones en la medición de estos marcadores, como la falta de un procedimiento estandarizado o materiales de referencia certificados. Además de las troponinas cardíacas y el electrocardiograma, medir la albúmina modificada por isquemia puede ayudar a excluir un síndrome coronario agudo en pacientes con baja probabilidad de isquemia miocárdica. La proteína fijadora de ácidos grasos-H es un marcador de necrosis útil en el diagnóstico precoz del infarto agudo de miocardio. En el pronóstico del síndrome coronario agudo, la proteína C reactiva, los péptidos natriuréticos y la mieloperoxidasa complementan el valor pronóstico de la troponina. El ligando soluble CD40 permite la clasificación e individualización del tratamiento del síndrome coronario agudo. Actualmente no hay suficiente evidencia para que ningún nuevo marcador sustituya a los que recomiendan las sociedades científicas ni se dispone de procedimientos de medición rápidos para algunos de ellos. Deben establecerse paneles utilizando la evidencia científica disponible y tomando como objetivo su contribución a una mejor evolución del paciente(AU)


Myocardical ischemia involves 1.3% of the patients attending emergency departments in Spain. The management of these patients is complex, due to the risk of an incorrect discharge diagnosis, the benefit of rapid revascularization and the excessive cost due to unnecessary admissions. There has been a significant improvement in the development of new cardiac biomarkers over the last ten years. Biomarkers traditionally used for identifying acute coronary syndrome were indicators of myocardial necrosis. This role has currently been expanded. There are still some limitations in the measurement of these biomarkers, due to lack of standardised assays or certified calibrators. Ischemia-modified albumin in conjunction with cardiac troponin and electrocardiogram, can help to rule out an acute coronary syndrome in patients with a low probability of having myocardical ischemia. Heart-type fatty acid-binding protein is a strong necrosis biomarker in the early diagnosis of acute myocardical infarction. C-reactive protein, natriuretic peptides and myeloperoxidase have been shown to complement cardiac troponin in the prognosis of acute coronary syndrome. Soluble CD40 ligand enables the identification of a subgroup of patients who will benefit from a treatment in acute coronary syndrome. There is currently not enough evidence to replace new biomarkers with any of these already been recommended by the scientific societies. Also, the assays of some of them are not sufficiently rapid. A multimarker strategy must be created to take into account the existing scientific-based evidence, with the aim of improving outcomes in patients(AU)


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Biomarcadores/análise , Síndrome Coronariana Aguda/diagnóstico , Cardiopatias/diagnóstico , Reação em Cadeia da Polimerase/tendências , Reação em Cadeia da Polimerase , Isquemia Miocárdica/diagnóstico , Peroxidase/análise , Peroxidase/isolamento & purificação , Peptídeos Natriuréticos/análise , Peptídeos Natriuréticos , Albumina Sérica/biossíntese , Biomarcadores Farmacológicos/análise , Biomarcadores Farmacológicos/química , Biomarcadores Farmacológicos/metabolismo
19.
J. physiol. biochem ; 64(1): 27-36, ene.-mar. 2008. tab, graf
Artigo em Inglês | IBECS | ID: ibc-61321

RESUMO

The purpose of this study was to compare the pro-antioxidant status in healthymen exposed to muscle-damaging resistance exercise, and to investigate the practicalapplication of Loverro’s coefficient (P/A ratio) to evaluate the presence of oxidativestress. Twenty-eight healthy men were assigned to two groups performed multi-joint(M) or single-joint (S) resistance exercise. The activities of superoxide dismutase(SOD), glutathione peroxidase (GPx) and catalase (CAT) as well as the concentrationof lipid peroxidation products (TBARS) in blood were evaluated. The P/A ratio wascalculated from the mean values of erythrocyte TBARS, SOD, CAT and GPx. Creatinekinase (CK) activity was used as a marker of muscle damage. The applied resistanceexercises triggered off the changes in pro-antioxidant ratio towards peroxidationwhich was proved by significant increase in erythrocyte TBARS concentrationin M (+25%) and S (+27%) groups. Plasma TBARS increased only after multi-jointresistance exercise and correlated with erythrocyte P/A ratio (r= 0.536, P< 0.01). Themulti-joint exercise caused decrease in SOD activity by 28% whereas the single-jointresistance exercise elevated enzyme activity by 20%. Activities of the other antioxidantenzymes changed simultaneously i.e. CAT activity increased by 14%-16%immediately after exercise, and GPx activity declined by 18%-34% during recoveryin M and S groups. Even though, all erythrocyte parameters significantly changedfollowing multi-joint and single-joint resistance exercises, the assessment of proantioxidantratio showed the considerable increase in P/A only in M group. In summary,an analysis of pro- and antioxidant parameters showed significant changes inresponse to muscle-damaging exercise and demonstrated the practical application ofP/A ratio to evaluate the risk of oxidative stress in athletes (AU)


No disponible


Assuntos
Humanos , Adulto , Masculino , Antioxidantes/metabolismo , Exercício Físico/fisiologia , Músculo Esquelético/lesões , Estresse Oxidativo/fisiologia , Contração Isométrica/fisiologia , Peroxidação de Lipídeos/fisiologia , Catalase/metabolismo , Músculo Esquelético/metabolismo , Eritrócitos/metabolismo , Peroxidase/metabolismo , Glutationa/metabolismo , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo
20.
J. physiol. biochem ; 63(3): 195-202, jul.-sept. 2007. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-72012

RESUMO

In aging liver oxidative stress increases due to the decrease in antioxidant bio-moleculessuch as estrogens which can be modified by hormonal replacement therapy(HRT). With this in mind, we hypothesized that age-related decline in steroidogenesismay be associated with the impairment of the antioxidant defense cells in liver,the increase in lipid peroxidation, hepatic dysfunction and histological changes;estrogens prevent all these changes induced by aging. 17â-estradiol treatment wasinitiated in 12 month-old Wistar rats, and continued until 18 months of age. Ourresults showed that 17Beta-estradiol (E2) level in the serum of the aged untreated ratswas reduced by –32% in 18 month-old rats compared to the young animals (4-month-old). The superoxide dismutase (SOD), catalase (CAT), and gluthatione peroxidase(GPX) activities were reduced by –47, –46, and –29% respectively in old ratliver. In addition, the TBARs in liver and hepatic dysfunction parameters in plasmasuch as gamma-glutamyl transferase (GGT), phosphatase alkalin (PAL) as well asbilirubin level increased significantly in old rats, and histological changes were investigated.In E2-treated rats, protective effects were observed. Indeed, 17Beta-estradiolattenuates all changes induced by aging. The 17Beta-estradiol level was higher in old E2-treated rats compared to the control rats. Moreover, the SOD, CAT and GPX activitieswere higher by +28, +15, and +11% respectively. This anti-aging effect of estrogenswas clarified by a lower level of lipid peroxidation and liver dysfunction parametersas well as by histological observation (AU)


No disponible


Assuntos
Animais , Ratos , Masculino , Estradiol/fisiologia , Estradiol/uso terapêutico , Estresse Oxidativo/fisiologia , Fígado/fisiologia , Catalase/síntese química , Catalase/fisiologia , Peroxidase/fisiologia , Hepatopatias/fisiopatologia , Insuficiência Hepática/fisiopatologia , Superóxido Dismutase/metabolismo , Superóxido Dismutase/uso terapêutico
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