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1.
Allergol. immunopatol ; 47(5): 467-476, sept.-oct. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-186521

RESUMO

Background: House dust mite (Dermataphagoides pteronyssinus) is a widespread risk factor in the development of asthma. CD4+ T lymphocytes have an important role in the pathogenesis of allergic asthma by polarizing to Th2 cells. Objective: We aimed to evaluate the immunoregulatory effects of dental follicle mesenchymal stem cells with and without IFN-γ stimulation on peripheral blood mononuclear cells of house dust mite sensitive asthmatic patients, and compared those with Dexamethasone as a systemic steroid. Material and methods: PBMC of asthmatic patients and healthy individuals separately cultured with or without DF-MSCs in the presence and absence of IFN-γ or Der p1 or Dexamethasone for 72h. CD4+ T proliferation, cell viability, CD4+CD25+FoxP3+ Treg cell frequency and cytokine profiles of PBMC were evaluated via flow cytometry. Results: DF-MSCs suppressed proliferation of CD4+ T lymphocytes (pCDmix < 0.01, pDerp1 < 0.01, pIFN < 0.005) by increasing the number of FoxP3 expressing CD4 + CD25 + T regulatory cells (pCDmix < 0.005, pDerp1 < 0.01, pIFN < 0.001) and suppressed lymphocyte apoptosis (pCDmix < 0.05, pDerp1< 0.05, pIFN < 0.05), while Dexamethasone increased the apoptosis and decreased Treg cell frequency in asthmatic patients. IFN-γ stimulation increased the suppressive effect of DF-MSCs and also enhanced the frequency of FoxP3 expressing CD4+CD25 + T regulatory cells. The cytokine levels were regulated by DF-MSCs by reducing IL-4 cytokine levels (pCDmix < 0.01, pDerp1 < 0.05, pIFN < 0.05) and upregulating IFN-γ levels (pCDmix < 0.01, pDerp1< 0.05, pIFN < 0.005) in asthmatic patients. Conclusion: IFN-γ stimulated DF-MSCs were found to have a high modulatory effect on CD4 + T cell responses, while Dexamethasone had an apoptotic effect on CD4+ T cells in asthmatic patients. DF-MSCs may be a new cell-based therapy option for allergic diseases including asthma


No disponible


Assuntos
Humanos , Animais , Masculino , Feminino , Adulto Jovem , Adulto , Asma/imunologia , Linfócitos T CD4-Positivos/imunologia , Saco Dentário/patologia , Dermatophagoides pteronyssinus/imunologia , Interferon gama/imunologia , Células-Tronco Mesenquimais/imunologia , Antígenos de Dermatophagoides/imunologia , Proteínas de Artrópodes/imunologia , Células Cultivadas , Imunidade Celular , Imunização
2.
Allergol. immunopatol ; 47(1): 38-42, ene.-feb. 2019. graf
Artigo em Inglês | IBECS | ID: ibc-180769

RESUMO

Introduction: Disseminated BCG infections among other complications of Bacillus Calmette-Guérin (BCG) vaccine are rare and have occurred in children with immunodeficiency disorders such as mendelian susceptibility to mycobacterial disease (MSMD) which could be due to defects in some elements of IL-12/IFN-γ axis. MSMD-causing mutations have been identified in 10 genes during the last two decades. Among them, mutations in the IL12Rβ1 and IFN gamma R1 genes constitute about 80% of recorded cases of MSMD syndrome. The aim of this study was to investigate IL-12RBeta1 and IFN- gammaR1 deficiencies in patients with disseminated BCG infection. Methods: This study was performed on 31 children with disseminated BCG infections who referred to children's medical center. Whole blood cell culture was performed in presence of BCG, IL-12 and IFN- gamma stimulators. The supernatants were assayed for IFN-gamma and IL-12p70 by ELISA method. In order to evaluate IL12Rbeta1 and IFN- gammaR1 receptors expression, flow cytometry staining was performed on the patients’ T-cells stimulated with PHA. Results: Flow cytometry staining of 31 Iranian patients with disseminated BCG infections with the average age of 43 months showed lack of the expression of IL-12RBeta1 and IFN- gamma R1 genes in PHA-T-cells of the nine and one patients, respectively in whom the incomplete production of IFN- gamma and IL-12 was reported by ELISA. Among these 10 patients, eight cases had related parents (80%). Conclusion: It is recommended that to avoid BCG complications, screening be performed for MSMD before BCG inoculation in individuals with positive family history of primary immunodeficiency diseases and inhabitants of areas with high frequency of consanguinity


No disponible


Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Vacina BCG/imunologia , Síndromes de Imunodeficiência/epidemiologia , Mutação/genética , Infecções por Mycobacterium/epidemiologia , Receptores de Interferon/genética , Interleucina-12/genética , Linfócitos T/imunologia , Células Cultivadas , Predisposição Genética para Doença , Imunização , Síndromes de Imunodeficiência/genética , Síndromes de Imunodeficiência/imunologia , Interferon gama/metabolismo , Interleucina-12/metabolismo , Irã (Geográfico)/epidemiologia , Infecções por Mycobacterium/genética , Infecções por Mycobacterium/imunologia
3.
Allergol. immunopatol ; 45(4): 333-338, jul.-ago. 2017. tab
Artigo em Inglês | IBECS | ID: ibc-165092

RESUMO

Background: A clear picture of interaction of Th1/Th2 cytokines in pathogenesis of chronic spontaneous urticaria (CSU), remains elusive. Impaired IFN-γ production and decreased levels of IL-2 have been reported. The aim of this study was to evaluate the association of Th1 cytokines; IL-2, IL-12 and IFN-γ polymorphisms with CSU. Methods: 90 patients with CSU and 140 age-sex matched subjects were included in this study. DNA samples were evaluated through PCR-SSP assay in order to detect single nucleotide polymorphisms of IL-12 (A/C -1188) or (rs3212227), IFN-γ (A/T UTR5644) or (rs2069717) and IL-2 (G/T -330 and G/T +166) or (rs2069762 and rs2069763). Results: G allele at -330 at promoter region of IL-2 gene was overrepresented in CSU. Heterozygotes (GT) at this locus and heterozygotes at +166 of IL-2 gene (GT) were more prevalent in CSU group. Additionally, the haplotype GT for loci -330 and +166 of IL-2 gene was powerfully associated with CSU (OR (95%CI) = 57.29 (8.43-112.7)). Conclusions: SNP at position -330 and +166 of IL-2 gene are differently expressed in CSU. The haplotype GT of IL-2 at -330 and +166 might confer vulnerability to a number of immunological disorders in Iranian region (AU)


No disponible


Assuntos
Humanos , Urticária/imunologia , Polimorfismo de Nucleotídeo Único/imunologia , Interleucina-2/análise , Interleucina-12/análise , Interferon gama/análise , Doença Crônica , Suscetibilidade a Doenças
4.
Arch. bronconeumol. (Ed. impr.) ; 52(9): 477-481, sept. 2016. tab
Artigo em Espanhol | IBECS | ID: ibc-155573

RESUMO

El uso de las pruebas de liberación de interferón-gamma para el diagnóstico de la infección tuberculosa está muy generalizado en España. Sin embargo, no se ha alcanzado un consenso respecto a su aplicación en situaciones clínicas específicas. Con el fin de redactar una guía de empleo en la práctica clínica, un grupo de expertos que incluyó a especialistas en enfermedades infecciosas, enfermedades respiratorias, microbiología, pediatría y medicina preventiva, junto con un experto en metodología, efectuaron una búsqueda sistemática en la literatura, sintetizaron los resultados, calificaron la calidad de las evidencias y formularon recomendaciones de acuerdo con la metodología Grading of Recommendations of Assessment Development and Evaluations. Este documento es una guía basada en la evidencia para el empleo de las pruebas de liberación de interferón-gamma en el diagnóstico de la infección tuberculosa en pacientes en riesgo de padecer tuberculosis o en los que se sospeche enfermedad activa. La guía será aplicable tanto en atención primaria y especializada como en salud pública


Interferon-gamma release assays are widely used for the diagnosis of tuberculosis infection in Spain. However, there is no consensus on their application in specific clinical scenarios. To develop a guide-line for their use, a panel of experts comprising specialists in infectious diseases, respiratory diseases, microbiology, pediatrics and preventive medicine, together with a methodologist, conducted a systematic literature search, summarized the findings, rated the quality of the evidence, and formulated recommendations following the Grading of Recommendations of Assessment Development and Evaluations methodology. This document provides evidence-based guidance on the use of interferon-gamma release assays for the diagnosis of tuberculosis infection in patients at risk of tuberculosis or suspected of having active disease. The guidelines will be applicable to specialist and primary care, and public health


Assuntos
Humanos , Masculino , Feminino , Interferon gama/análise , Testes de Liberação de Interferon-gama/instrumentação , Testes de Liberação de Interferon-gama/métodos , Testes de Liberação de Interferon-gama , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/tratamento farmacológico , Tuberculose Latente/diagnóstico , Infecções Bacterianas/complicações , Infecções Bacterianas/diagnóstico , Tuberculose Latente/prevenção & controle , Medicina Preventiva/métodos , Doenças Respiratórias/prevenção & controle , Atenção Primária à Saúde/métodos , Atenção Primária à Saúde/tendências , Atenção Primária à Saúde , Saúde Pública/métodos
6.
Allergol. immunopatol ; 44(4): 303-306, jul.-ago. 2016. tab
Artigo em Inglês | IBECS | ID: ibc-154431

RESUMO

Background: Cytokines, including interleukin-2 (IL-2) and interferon-gamma (IFN-γ), seem to play a role in the pathogenesis of juvenile idiopathic arthritis (JIA). The aim of this study was to investigate the associations of IL-2 and IFN-γ single nucleotide polymorphisms (SNPs) with susceptibility to JIA in an Iranian population. Methods: enomic DNA of 54 Iranian patients with JIA and 139 healthy unrelated controls were typed for IL-2 (G/T at −330 and +166) as well as IFN-γ gene (A/T at +874), using polymerase chain reaction with sequence-specific primers method, and compared between patients and controls. Results: A significantly higher frequency of the IL-2 −330 GG genotype (p<0.01) was found in the JIA patients compared to the controls. However, the GT genotype at the same position was notably lower than in controls (p<0.01). Moreover, IL-2 (−330, +166) GT haplotype was more frequent in patients with JIA in comparison with controls. No significant differences was observed between the two groups of case and control for IL-2 (G/T at +166) and IFN-γ (A/T at +874) SNPs. Conclusion: The results of the current study suggest that certain SNPs of IL-2 gene have association with individuals’ susceptibility to JIA. However, further investigations are required to confirm the results of this study (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Interleucina-2/análise , Interleucina-2/imunologia , Interferon gama/imunologia , Interferon gama , Artrite Juvenil/imunologia , Artrite Juvenil/fisiopatologia , Reação em Cadeia da Polimerase/métodos , Complexo Principal de Histocompatibilidade/imunologia , Estudos de Casos e Controles
7.
Allergol. immunopatol ; 44(4): 314-321, jul.-ago. 2016. tab, graf
Artigo em Inglês | IBECS | ID: ibc-154433

RESUMO

Background: Since gamma interferon release assays (IGRAs) cannot differentiate between active tuberculosis and latent tuberculosis infection (LTBI), development of rapid and specific diagnosis tools are essential for discriminating between active tuberculosis (TB) from LTBI. Both IGRAs are based on Mycobacterium tuberculosis-specific antigens, namely, early secretory antigenic target 6 (ESAT-6) and 10kDa culture filtrate (CFP-10). The aim of this study was to evaluate the potential value of IL-2 secretion by whole blood cells after stimulation with rESAT-6 and rCFP-10 for discriminating between active and latent tuberculosis. Methods: Interleukin-2 and IFN-γ were measured after blood stimulation of 90 cases (30 with active TB, 30 with LTBI and 30 healthy controls) with recombinant ESAT-6 and CFP-10. Receiver operating characteristic (ROC) curve analysis was conducted to determine the best IL-2 and IFN-γ result thresholds in discriminating between cases with active or latent TB, and the corresponding sensitivity and specificity were recorded. Results: The IFN-γ release assay demonstrated a good sensitivity and specificity (sensitivity 83-84% and specificity 92%) for diagnosis of tuberculosis. The discrimination performance of IL-2 assay (assessed by the area under ROC curve) between LTBI and patients with active TB were 0.75 and 0.8 following stimulation with rESAT-6 and rCFP-10, respectively. Maximum discrimination was reached at a cut-off of 11.6pg/mL for IL-2 after stimulation with recombinant rESAT-6 with 72% sensitivity and 79% specificity and 10.7pg/mL for IL-2 following stimulation with rCFP-10 with 75% sensitivity and 79% specificity, respectively. Conclusion: This study demonstrates that rESAT-6 and rCFP-10 can provide a sensitive and specific diagnosis of TB. In addition, it was shown that IL-2 may be serving as a marker for discriminating LTBI and active TB (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Interleucina-2/análise , Interleucina-2/imunologia , Interferon gama/análise , Interferon gama/imunologia , Tuberculose/diagnóstico , Tuberculose/imunologia , Tuberculose/patologia , Tuberculose Latente/diagnóstico , Tuberculose Latente/imunologia , Ensaio de Imunoadsorção Enzimática/métodos
8.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 34(5): 303.e1-303.e13, mayo 2016. tab
Artigo em Inglês | IBECS | ID: ibc-152544

RESUMO

INTRODUCTION: Interferon-gamma release assays are widely used for the diagnosis of tuberculosis infection in low-prevalence countries. However, there is no consensus on their application. The objective of this study was to develop guidelines for the use of interferon-gamma release assays in specific clinical scenarios in Spain. METHODS: A panel of experts comprising specialists in infectious diseases, respiratory diseases, microbiology, pediatrics and preventive medicine, together with a methodologist, formulated the clinical questions and outcomes of interest. They conducted a systematic literature search, summarized the evidence and rated its quality, and prepared the recommendations following the GRADE (Grading of Recommendations of Assessment Development and Evaluations) methodology. RESULTS: The panel prepared recommendations on the use of interferon-gamma release assays for the diagnosis of tuberculosis infection in the contact-tracing study (both adults and children), health care workers, immunosuppressed patients (patients infected with human immunodeficiency virus, patients with chronic immunomediated inflammatory diseases due to start biological therapy and patients requiring organ transplant) and for the diagnosis of active tuberculosis. Most recommendations were weak, mainly due to the lack of good quality evidence to balance the clinical benefits and disadvantages of the interferon-gamma release assays as compared with the tuberculin skin test. CONCLUSION: This document provides evidence-based guidance on the use of interferon-gamma release assays for the diagnosis of tuberculosis infection in patients at risk of tuberculosis or with suspicion of active disease. The guidelines will be applicable in specialist and primary care and in public health settings


INTRODUCCIÓN: Las técnicas de detección in vitro de interferón-gamma (IGRA, del inglés interferon-gamma release assays) están ampliamente implantadas para el diagnóstico de infección tuberculosa en países de baja prevalencia. Sin embargo, no hay consenso sobre su aplicación. El objetivo fue desarrollar una guía de práctica clínica para el uso de los IGRA en los diferentes escenarios clínicos en España. MÉTODOS: Un grupo de expertos compuesto por especialistas en enfermedades infecciosas, enfermedades respiratorias, microbiología, pediatría y medicina preventiva, junto con un metodólogo formularon las preguntas clínicas y los desenlaces de interés, llevaron a cabo una búsqueda sistemática de la literatura, sintetizaron la evidencia y graduaron su calidad, y formularon las recomendaciones siguiendo la metodología Grading of Recommendations of Assessment Development and Evaluations(GRADE). RESULTADOS: El grupo de trabajo formuló las recomendaciones sobre el uso de los IGRA para el diagnóstico de infección tuberculosa en el estudio de contactos (adultos y niños), trabajadores sanitarios, pacientes inmunosuprimidos (pacientes infectados por el virus de la inmunodeficiencia humana, pacientes afectos de enfermedades inflamatorias inmunomediadas candidatos a terapias biológicas y pacientes que requieren trasplante de órganos), y en el diagnóstico de enfermedad tuberculosa activa. La mayor parte de las recomendaciones fueron débiles, principalmente debido a la falta de evidencia de calidad para establecer un balance entre beneficios y daños de los IGRA en comparación con la prueba de la tuberculina. CONCLUSIÓN: Este documento proporciona una guía basada en la evidencia para el uso de los IGRA en el diagnóstico de infección tuberculosa en pacientes en riesgo de tuberculosis o con sospecha de enfermedad activa. Esta guía es aplicable en la atención especializada y primaria, y salud pública


Assuntos
Humanos , Tuberculose/diagnóstico , Tuberculose Latente/diagnóstico , Mycobacterium tuberculosis/isolamento & purificação , Interferon gama/análise , Busca de Comunicante/métodos , Fatores de Risco
9.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 34(5): 304-308, mayo 2016. tab
Artigo em Inglês | IBECS | ID: ibc-152545

RESUMO

Interferon-gamma release assays are widely used for the diagnosis of tuberculosis infection in Spain. However, there is no consensus on their application in specific clinical scenarios. To develop a guideline for their use, a panel of experts comprising specialists in infectious diseases, respiratory diseases, microbiology, pediatrics and preventive medicine, together with a methodologist, conducted a systematic literature search, summarized the findings, rated the quality of the evidence, and formulated recommendations following the GRADE (Grading of Recommendations of Assessment Development and Evaluations) methodology. This document provides evidence-based guidance on the use of interferon-gamma release assays for the diagnosis of tuberculosis infection in patients at the risk of tuberculosis or suspected of having active disease. The guidelines will be applicable to specialist and primary care, and public health


Las técnicas de detección in vitro de interferón-gamma (IGRA, del inglés interferon-gamma release assays), están ampliamente implantadas para el diagnóstico de infección tuberculosa en España. Sin embargo, no hay consenso sobre su aplicación en diferentes escenarios clínicos. Para desarrollar una guía de práctica clínica sobre su uso, un grupo de trabajo compuesto por especialistas en enfermedades infecciosas, neumología, microbiología, pediatría y medicina preventiva, junto con un metodólogo, llevaron a cabo una búsqueda sistemática de la literatura, sintetizaron la evidencia y gradaron su calidad, y formularon las recomendaciones siguiendo el método GRADE (Grading of Recommendations of Assessment Development and Evaluations). Este documento proporciona una guía basada en la evidencia para el uso de los IGRA para el diagnóstico de infección tuberculosa en pacientes en riesgo de tuberculosis o con sospecha de enfermedad activa. Esta guía es aplicable en la atención especializada y primaria, y salud pública


Assuntos
Humanos , Tuberculose/diagnóstico , Tuberculose Latente/diagnóstico , Mycobacterium tuberculosis/isolamento & purificação , Interferon gama/análise , Busca de Comunicante/métodos , Fatores de Risco
10.
Allergol. immunopatol ; 43(5): 456-460, sept.-oct. 2015. tab, graf
Artigo em Inglês | IBECS | ID: ibc-141105

RESUMO

Introduction: Diagnosis of specific molecular defects of Mendelian susceptibility to mycobacterial diseases (MSMD) patients is important with respect to their clinical outcomes and their response to therapy. The aim of this study was to perform functional tests on blood samples of a group of patients who were suspected of having MSMD. Methods: This study was performed on 11 cases who had mycobacterial infections and suspected MSMD. Whole blood cell culture was performed in presence of different stimulators. The supernatants were assayed for IFN-γ, IL-12p40 by ELISA method. Results: All patients presented with complications of BCG vaccine in the form of localised lymphadenitis or disseminated BCG infection and chronic mycobacterial osteomyelitis. Infections with Salmonellaspecies occurred in two patients. In-vitro studies showed that 10 cases had impaired response to IL-12. However, the baseline levels of IL-12p40 were normal, while one of our patients may have a potential IFN-γ signalling defect or an IL-12p40 defect. Conclusions: Early detection of MSMD and commencing of appropriate combination therapy could prevent severe or even fatal complications of uncontrolled mycobacterial infections (AU)


No disponible


Assuntos
Criança , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Infecções por Mycobacterium/tratamento farmacológico , Vacina BCG/uso terapêutico , Interferon gama , Interleucina-12 , Subunidade p40 da Interleucina-12 , Subunidade beta 1 de Receptor de Interleucina-12/deficiência , Monitoramento Epidemiológico/tendências , Infecções por Mycobacterium não Tuberculosas , Infecções por Salmonella , Tuberculose , Ensaio de Imunoadsorção Enzimática , Mycobacterium bovis , Doenças do Sistema Imunitário , Suscetibilidade a Doenças , Irã (Geográfico)/epidemiologia
11.
Allergol. immunopatol ; 43(5): 482-486, sept.-oct. 2015. tab, graf
Artigo em Inglês | IBECS | ID: ibc-141110

RESUMO

Introduction: Asthma is an inflammatory disorder of the airways associated with bronchial hyperresponsiveness, airway obstruction, and increased mucus production, with a predominance of type 2 immune response (Th2). According to the hygiene hypothesis, exposure to environmental bacterial lipopolysaccharide (LPS) may induce a type 1 immune response (Th1), modulating the development of asthma. Objective: In this study we investigated cytokine production by peripheral blood mononuclear cells (PBMC) from children and adolescents with severe asthma, in response to LPS stimulation in vitro. Materials and methods: 26 children were selected: 13 severe asthmatics and 13 healthy controls, aged between 5 and 18 years. They were evaluated through routine medical history, physical examination and lung function test to diagnose severe asthma. Allergy status was confirmed by skin prick test and specific IgE assay. We collected blood samples to analyse in vitro LPS-induced cytokines release by PBMC. Results: PBMC from severe asthmatic children produced lower levels of IL-12p70 in basal conditions and after 12 and 24 h stimulation with LPS compared to healthy controls. PBMC from severe asthmatic children produced lower levels of IL-4 after 24 h LPS stimulation compared to healthy controls. PBMC from severe asthmatic children produced more levels IL-17 and IL-10 after stimulus with LPS compared to healthy controls. The release of IFN-γ, IL-5 and TNF-α by PBMC from severe asthmatic children was similar to healthy controls. Conclusion: Our results demonstrate that LPS directly influence the cytokine profile of PBMC in children with severe asthma. These observations may be potentially helpful in developing new treatment strategies (AU)


No disponible


Assuntos
Adolescente , Criança , Feminino , Humanos , Masculino , Asma/imunologia , Células Th2 , Células Th1 , Leucócitos Mononucleares , Interleucina-4 , Interleucina-12 , Interferon gama , Linfotoxina-alfa , Lipopolissacarídeos , Monitoramento Epidemiológico/tendências , Interleucina-5 , Interleucina-10 , Interleucina-17 , Citocinas , Hipersensibilidade , Brasil/epidemiologia
12.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 33(2): 105-109, feb. 2015. tab
Artigo em Espanhol | IBECS | ID: ibc-133234

RESUMO

INTRODUCCIÓN: El objetivo del presente trabajo fue medir la expresión de interferón gamma en la infección por VPH y por Chlamydia trachomatis en pacientes con lesión intraepitelial escamosa. MÉTODO: Se incluyeron 100 muestras de pacientes diagnosticadas por colposcopía, con y sin lesión intraepitelial escamosa, en quienes se efectuó el diagnóstico de infección por VPH y/o C. trachomatis. Se cuantificó la expresión relativa de interferón gamma con la prueba de transcriptasa reversa-PCR en tiempo real (RT-PCR). RESULTADOS: Las unidades relativas de la expresión de interferón gamma fueron de 13, 1,8 y 0,3 en la coinfección por VPH y C. trachomatis, en la infección por VPH y en la infección por C. trachomatis, respectivamente. CONCLUSIÓN: La infección por VPH y por C. trachomatis puede constituir un factor estimulante de la expresión de interferón gamma


INTRODUCTION: The aim of this study was to mesure the expression of gamma interferon in HPV and Chlamydia trachomatis infection in squamous intraepithelial lesions. METHOD: Samples from 100 patients diagnosed by colposcopy with or without squamous intraepithelial lesions were used in the present study. Each patient was found to be infected by HPV and C. trachomatis. Relative gamma interferon mRNA expression was assessed using a real-time reverse transcriptase PCR assay (RT-PCR). RESULTS: The relative units of expression of gamma interferon mRNA were 13, 1.8 and 0.3, for HPV and C. trachomatis co-infection, or HPV or C. trachomatis infection, respectively. CONCLUSIÓN: HPV and C. trachomatis could overstimulate the expression of gamma interferon


Assuntos
Humanos , Interferon gama/análise , Infecções por Papillomavirus/imunologia , Infecções por Chlamydia/imunologia , Neoplasias do Colo do Útero/microbiologia , Papillomaviridae/patogenicidade , Chlamydia trachomatis/patogenicidade , Coinfecção/microbiologia , Doenças Sexualmente Transmissíveis/imunologia , Neoplasia Intraepitelial Cervical/microbiologia
13.
Arch. bronconeumol. (Ed. impr.) ; 50(11): 484-489, nov. 2014. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-129841

RESUMO

El presente artículo analiza el concepto de lesiones fibróticas inactivas de presumible origen tuberculoso (old healed tuberculosis), su definición por sus características radiológicas y la presencia de prueba de la tuberculina (TST) positiva. Se revisa el fundamento basado en la evidencia de la indicación de tratamiento de infección tuberculosa latente en estos casos, el riesgo de reactivación en la literatura antigua y reciente, así como los problemas que plantea el diagnóstico diferencial con la tuberculosis activa con bacteriología negativa. Se consideran los datos sobre prevalencia de lesiones fibróticas en la literatura reciente. Se analiza el posible papel de las técnicas de Interferon Gamma Release Assay (IGRA) versus TST, así como otras técnicas moleculares de detección antigénica en esputo que pueden ayudar a hacer el diagnóstico. Se analizan las actuales indicaciones de quimioprofilaxis, las diferentes opciones y se proponen algoritmos diagnósticos y terapéuticos basados en la estratificación del riesgo según la edad y otros factores, para manejar las lesiones radiológicas que plantean diagnóstico diferencial entre lesión fibrótica inactiva y tuberculosis pulmonar con bacteriología negativa


This article analyzes the concept of inactive fibrotic lesions of presumed tuberculous origin (old healed tuberculosis), defined by radiological characteristics and a positive tuberculin skin test (TST), and we examine the evidence-based foundation for the indication of treatment of latent tuberculosis infection in these cases. We explore the risk of reactivation in older and recent literature, and the problems raised by the differential diagnosis with active tuberculosis with negative bacteriology. We also analyze data on the prevalence of fibrotic lesions in the recent literature. We examine the possible role of Interferon Gamma Release Assays (IGRAs) versus TST and other molecular antigen detection techniques in sputum that can aid in establishing the diagnosis and we discuss the current indications for chemoprophylaxis and the different options available. We propose diagnostic guidelines and therapeutic algorithms based on risk stratification by age and other factors in the management of radiological lesions that raise a differential diagnosis between fibrotic lesions and active pulmonary tuberculosis with negative bacteriology


Assuntos
Humanos , Tuberculose Pulmonar/diagnóstico , Fibrose Pulmonar/diagnóstico , Tuberculose Latente/diagnóstico , Teste Tuberculínico , Fatores de Risco , Interferon gama
14.
J. investig. allergol. clin. immunol ; 24(2): 87-97, mar.-abr. 2014. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-122268

RESUMO

Background: Specific oral tolerance induction (SOTI) for IgE-mediated food allergy (IFA) can be successfully achieved using interferon gamma (classic SOTI). Objective: In this study, a tolerable dose was introduced during tolerance induction with interferon gamma (dual SOTI), and its effectiveness was evaluated. Methods: The study population comprised 25 IFA patients. Blood samples were taken for analysis, including complete blood count with differential counts of eosinophils, serum total IgE levels, and specific IgE for allergenic foods. Skin prick tests were conducted with the allergens. Oral food challenges were performed to diagnose IFA. Ten patients received dual SOTI, 5 received classic SOTI, 5 received SOTI without interferon gamma (original SOTI), and 5 were not treated (controls). Results: Patients treated with dual SOTI and classic SOTI using interferon gamma became tolerant to the allergenic food. The tolerable dose was introduced successfully in dual SOTI. It was difficult to proceed with the same dosing protocol used for classic SOTI in cases treated with original SOTI. Following dual SOTI, the systemic reaction to oral intake subsided, but the local skin reaction to contact with the allergenic food persisted. Conclusions: Dual SOTI is an improved protocol for SOTI using interferon gamma for IFA. The local skin reaction and systemic reaction to oral intake were dissociated following dual SOTI. In cases of food allergy, tolerance appears to result from desensitization to allergens (AU)


Antecedentes: La inducción de tolerancia oral específica (SOTI) para la alergia alimentaria mediada por IgE (IFA) se ha logrado empleando IFN-γ (SOTI mejorada). Objetivo: Se evaluó la eficacia de la administración de dosis tolerables de alimento junto con IFN-g durante la inducción de tolerancia (SOTI doble). Material y métodos: Se incluyeron 25 pacientes con IFA. Se analizaron muestras de sangre, realizando un hemograma completo con recuento diferencial de eosinófilos, niveles de IgE total en suero, y de IgE específica, así como pruebas cutáneas para los alimentos implicados. El diagnóstico final de IFA se realizó mediante provocación oral controlada. Diez pacientes recibieron SOTI con dosis tolerables de alimento e IFN-γ (SOTI doble), 5 recibieron SOTI usando solo IFN-γ (SOTI mejorada), 5 recibieron SOTI sin IFN-γ (SOTI convencional), y 5 no fueron tratados (grupo control).Resultados: Los pacientes tratados con SOTI doble y SOTI mejorada utilizando IFN-γ, alcanzaron la tolerancia del alimento alergénico. La dosis tolerable se alcanzó con éxito en la SOTI doble. Fue difícil el aplicar el mismo protocolo con la misma dosis en la SOTI mejorada y en los casos tratados con SOTI sin IFN-γ (SOTI convencional). Mediante SOTI doble, las reacciones sistémicas a la ingesta oral disminuyeron; sin embargo, la reacción local cutánea al contacto con el alimento alergénico permaneció invariable. Conclusiones: La SOTI doble es un protocolo mejorado para SOTI utilizando IFN-γ para el tratamiento de la IFA. La reacción local de la piel y la reacción sistémica a la ingesta oral son independientes tras la realización de la SOTI doble. La inducción de tolerancia parece ser el resultado de la desensibilización a los alérgenos (AU)


Assuntos
Humanos , Hipersensibilidade Alimentar/terapia , Dessensibilização Imunológica , Interferon gama/uso terapêutico , Hipersensibilidade Imediata/terapia , Anafilaxia/terapia , Testes de Química Clínica/métodos , Tolerância Imunológica/imunologia , Hipersensibilidade Tardia/terapia , Alérgenos/uso terapêutico
15.
Med. clín (Ed. impr.) ; 140(7): 289-295, abr. 2013. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-110405

RESUMO

Fundamento y objetivos: El objetivo del estudio fue comparar la prueba de la tuberculina (PT) y el QuantiFERON-TB® Gold In-Tube (QFT) en el diagnóstico de la infección latente tuberculosa (ILT) en una población de contactos de enfermos con tuberculosis pulmonar, y analizar la influencia de distintas variables en la discordancia. Pacientes y método: Entre marzo de 2008 y septiembre de 2010, de una población de 300.000 habitantes del País Vasco, se analizaron todos los contactos de pacientes con tuberculosis pulmonar. A todos se les realizó la PT y se midió el valor del QFT. Se analizaron diferentes variables sociodemográficas y la vacunación, analizándose la discordancia entre ambas pruebas. Resultados: Un total de 704 sujetos fueron incluidos en el estudio, con una edad media de 27 años. De ellos, 397 estaban vacunados, siendo la proporción similar entre autóctonos y extranjeros. El incremento de la edad hasta los 59 años (odds ratio [OR] 10,53; p<0,001), ser extranjero (OR 2,71; p=0,02) y la vacunación (OR 4,22; p<0,001) fueron variables predictoras de la discordancia entre una PT positiva y un QFT negativo. Conclusiones: Parece que el QFT, solo o combinado con la PT, es un método seguro para el diagnóstico de la ILT y que su utilización contribuiría a una selección más específica de los individuos que necesitan un tratamiento preventivo (AU)


Background and objectives: Our objetive is to compare the tuberculin skin test (TST) and the QuantiFERON-TB® Gold In-Tube (QFT) in the diagnosis of latent tuberculosis infection (LTI) in a population of contacts of patients with pulmonary tuberculosis, and to analyze the influence of different variables in the discordance. Patients and method: From March 2008 to September 2010, among a population of 300,000 inhabitants of the Basque Country, we analyzed all contacts of patients with pulmonary tuberculosis. All patients underwent the TST and the value of QFT was measured. Sociodemographic variables and vaccination were examined and we analyzed the discordance between the 2 tests. Results: Seven hundred and four were included in the study, with a mean age of 27 years. Of these, 397 were vaccinated, with similar proportion between native and foreign. Increasing the age to 59 years (odds ratio [OR] 10.53, P<.001), being foreign (OR 2.71, P=.02) and vaccination (OR 4.22, P<.001) were predictors of the discordance between a positive TST and negative QFT. Conclusions: It seems that the QFT, alone or combined with the TST, is a safe method for the diagnosis of LTI and its use would contribute to a more specific selection of individuals who would need preventive treatment (AU)


Assuntos
Humanos , Teste Tuberculínico/métodos , Tuberculose/diagnóstico , Interferon gama , Busca de Comunicante/métodos , Mycobacterium tuberculosis/isolamento & purificação , Transmissão de Doença Infecciosa/estatística & dados numéricos
16.
Actas dermo-sifiliogr. (Ed. impr.) ; 103(10): 880-886, dic. 2012. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-107749

RESUMO

Las terapias biológicas representan una alternativa de indudable eficacia en la psoriasis moderada y grave. Sin embargo, existe una asociación entre el tratamiento con anti-TNF-alfa y la reactivación de la infección tuberculosa. La tuberculina se utiliza como herramienta para el diagnóstico de la infección tuberculosa, pero presenta una baja especificidad en pacientes vacunados con la BCG (Mycobacterium bovis bacilo de Calmette-Guérin) y una baja sensibilidad en pacientes con alteraciones de la inmunidad celular. En este sentido se han desarrollado diferentes metodologías in vitro que incorporan antígenos específicos de Mycobacterium tuberculosis para estimular células T sensibilizadas y detectar posteriormente IFN-y liberado para el diagnóstico in vitro de la infección tuberculosa. Los resultados obtenidos hasta ahora muestran a estas como una alternativa real a la tuberculina, ya que presentan una mayor especificidad y sensibilidad. Estas técnicas, además, están demostrando un elevado valor predictivo negativo que hace que nos podamos plantear a corto-medio plazo su utilización sin necesidad de combinarla con la tuberculina (AU)


Although there is no doubt that biologic agents are an effective alternative for the treatment of moderate and severe psoriasis, anti-tumor necrosis factor alpha therapy has been associated with reactivation of latent tuberculosis infection. Tuberculin skin testing (TST) is used to diagnose tuberculosis infection but it has low specificity in patients who have received the Mycobacterium bovis BCG vaccine and low sensitivity in patients with altered cell-mediated immunity. In vitro assays based on the detection of interferon y released by T cells stimulated by specific Mycobacterium tuberculosis antigens have emerged as an option for the diagnosis of tuberculosis infection. The results to date show that they are a viable alternative to TST thanks to their higher specificity and sensitivity. Furthermore, these assays are also proving to have high negative predictive value, meaning that we might be able to use them without TST in the short to medium term (AU)


Assuntos
Humanos , Psoríase/tratamento farmacológico , Tuberculose Cutânea/epidemiologia , Terapia Biológica , Interferon gama/análise , Psoríase/complicações , Teste Tuberculínico , Mycobacterium tuberculosis/isolamento & purificação , Valor Preditivo dos Testes
17.
Arch. bronconeumol. (Ed. impr.) ; 48(5): 144-149, mayo 2012. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-101448

RESUMO

Introducción: El diagnóstico de la tuberculosis como infección latente (TBL) en pacientes con artritis reumatoide (AR) ha cobrado importancia con la introducción de agentes anti-factor de necrosis tumoral alfa (anti-TNF-Alfa) y la aparición de casos de tuberculosis activa en estos pacientes. La prueba cutánea de la tuberculina (PT) tiene un valor limitado en pacientes con AR. Se están estudiando las pruebas basadas en la liberación de interferón-gamma (IFN-epsilon), pero su papel no ha sido bien establecido para este grupo de pacientes. Objetivos: Comparar el diagnóstico de TBL en pacientes con AR con respuesta inmune celular a la PT y T.SPOT-TB. Además, determinar los hallazgos de un estudio de tomografía compatibles con TBL. Métodos: Evaluación clínica, PT, T.SPOT-TB y tomografía computarizada de alta resolución (TCAR) en un grupo de pacientes con AR del Hospital de la Universidad Federal de Goiás. Resultados: La respuesta a la PT fue inferior en los pacientes con AR (13,5%), en relación a lo esperado en la población general. El T.SPOT-TB identificó un número mayor de pacientes con TBL al compararlo con la PT (36,8%). La TCAR mostró cambios compatibles con TBL en el 52,9% de los pacientes, incluyendo 8 de los 11 pacientes con PT negativo y T.SPOT-TB. Conclusiones: La PT por sí misma no es suficiente para diagnosticar la TBL. Un mayor número de resultados positivos se obtuvieron con el T.SPOT-TB, si se lo compara con la PT, aunque fue negativo en un gran porcentaje de pacientes con hallazgos consistentes entre la tomografía y la TBL. La TCAR está disponible en la mayoría de los grandes centros y podría ser incorporada en la estrategia para el diagnóstico de TBL en pacientes con AR(AU)


Introduction: The diagnosis of latent tuberculosis (LTB) in patients with rheumatoid arthritis (RA) has become important with the introduction of anti-tumor necrosis factor (anti-TNF-Alfa) agents and the appearance of active tuberculosis cases in these patients. The tuberculin skin test (TST) has limited value in patients with RA. Tests based on the release of interferon-gamma (IFN-epsilon) are being studied, but their role has not been well established for this group of patients. Objectives: To compare the diagnosis of LTB in patients with RA by using cellular immune response to the TST and T.SPOT-TB. Additionally, findings of tomography studies compatible with LTB were used. Methods: Clinical evaluation, TST, T.SPOT-TB and high-resolution computed tomography (HRCT) in a group of patients with RA at the University Hospital of the Federal University of Goiás. Results: Response to the TST was lower in patients with RA (13.5%) compared to the predicted values of the general population. T.SPOT-TB identified a higher number of patients with LTB than the TST (36.8%). HRCT showed changes compatible with LTB in 52.9% of the patients, including 8 of the 11 patients with negative TST and T.SPOT-TB. Conclusions: The TST by itself is insufficient to diagnose LTB. A higher number of positive results were obtained with T.SPOT-TB when compared to the TST. Nevertheless, it was negative in a large percentage of patients with tomography findings consistent with LTB. HRCT is readily available in most large health-care centers and it could be incorporated into the diagnostic strategy for LTB in patients with RA(AU)


Assuntos
Humanos , Masculino , Feminino , Artrite Reumatoide/complicações , Artrite Reumatoide/tratamento farmacológico , Tuberculose Latente/diagnóstico , Tuberculose Latente/terapia , Fator de Necrose Tumoral alfa/efeitos adversos , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/uso terapêutico , Tomografia Computadorizada Espiral , Teste Tuberculínico/métodos , Teste Tuberculínico , Interferon gama/antagonistas & inibidores , Interferon gama
18.
Clin. transl. oncol. (Print) ; 14(4): 271-279, abr. 2012.
Artigo em Inglês | IBECS | ID: ibc-126187

RESUMO

BACKGROUND: Immunotherapy using autologous dendritic cell (DC) vaccination has not been systematically evaluated in osteosarcoma. We therefore conducted a phase I trial to assess feasibility, safety and tumour-specific immune responses in patients with relapsed disease. PATIENTS AND METHODS: Of 13 recruited patients with relapsed osteosarcoma, 12 received 3 weekly vaccines of autologous DCs matured with autologous tumour lysate and keyhole limpet haemocyanin (KLH), to a maximum of 6 vaccinations. An additional 3 paediatric patients afflicted with other tumour types and with relapsed disease received vaccines generated with identical methodology. Immune responses were assessed using an ELISpot assay for the detection of interferon gamma, whilst interleukin-2 and granzyme B were additionally assessed in cases where interferon-γ responses were induced. RESULTS: In total 61 vaccines, of homogeneous maturation phenotype and viability, were administered with no significant toxicity. Only in 2 out of 12 treated osteosarcoma cases was there an induction of specific T-cell immune response to the tumour, whilst a strong but non-specific immune response was induced in 1 further osteosarcoma patient. Immune response against KLH was induced in only 3 out of 12 osteosarcoma patients. In contrast, three additional non-osteosarcoma patients showed significant T-cell responses to vaccine. CONCLUSION: We have shown the strategy of DC vaccination in relapsed osteosarcoma is safe and feasible. However, significant anti-tumour responses were induced in only 2 out of 12 vaccinated patients with no evidence of clinical benefit. Comparison of results with identically treated control patients suggests that osteosarcoma patients might be relatively insensitive to DC-based vaccine treatments (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Vacinas Anticâncer , Linfócitos T/citologia , Células Dendríticas/citologia , Osteossarcoma/imunologia , Imunoterapia/métodos , Estudos de Viabilidade , Hemocianinas/química , Osteossarcoma/terapia , Osteossarcoma/metabolismo , Fenótipo , Recidiva , Resultado do Tratamento , Granzimas/biossíntese , Interferon gama/biossíntese , Interleucina-2/biossíntese
20.
Inmunología (1987) ; 30(2): 36-44, abr.-jun. 2011. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-109192

RESUMO

La integridad del eje interleucina 12/interferón gamma (IL-12/INF-g) es esencial para un correcto control de la infección por Mycobacterium tuberculosis. El objetivo del presente estudio fue evaluar si la alta incidencia de tuberculosis (TB) en Galicia (España) podría estar relacionada con una respuesta alterada en el eje IL-12/INF-g en los pacientes con TB. Se estudió a 20 enfermos con TB y 21 controles sanos: 7 con prueba de la tuberculina positiva (PT+) y 14 con prueba de la tuberculina negativa (PT–). El estudio del eje IL-12/INF-g se realizó mediante la determinación de los niveles de INF-g y la expresión celular de los receptores IL-12Rb1 e INF-gR1 en linfocitos y monocitos, respectivamente. Los ensayos se realizaron tanto a nivel basal, como tras estimulación mediante incubación in vitro de células mononucleares de sangre periférica con fitohemaglutinina (PHA) y derivado proteico purificado (PPD). Se analizaron y compararon las respuestas de los enfermos y de los controles sanos. La estimulación in vitro con PHA y PPD incrementó de forma significativa los niveles de INF-g, y la expresión celular de los marcadores IL-12Rb1 y INF-gR1 respecto a los niveles basales, tanto en el grupo de enfermos con TB, como en el de controles sanos (con PT+ y PT–). Todo ello es indicativo de una respuesta inmunitaria adecuada en todos los grupos, en los que la funcionalidad del eje IL-12/INF-g está conservada en los pacientes analizados con TB en Galicia. Aunque nuestro estudio no ha analizado todas las vías de alteración posibles del eje IL-12/INF-g, las altas tasas de enfermedad que históricamente se observan en esta comunidad no parecen estar causadas por una disfunción de la respuesta inmunitaria a este nivel (AU)


The integrity of the interleukin 12/interferon gamma (IL-12/INF-g) axis has been shown to be essential in the control of the Mycobacterium tuberculosis infection. The aim of this study was to assess whether the high incidence of tuberculosis (TB) in Galicia, Spain, could be related to an altered response in the IL-12/INF-g axis in patients with TB. We studied 20 TB patients and 21 healthy controls: 7 with positive Tuberculin Skin test (TST+) and another 14 with TST–. The study of the IL-12/INF-g axis was conducted by the analysis of INF-glevels (in serum and supernatants of non-activated and activated cells) and by the cellular expression of IL-12Rb1 and INF-gR1 in lymphocytes and monocytes, respectively. The assays were performed at baseline levels and after in vitro stimulation of peripheral blood mononuclear cells with phytohaemagglutin in (PHA) and purified protein derivative (PPD). Responses in patients and in healthy controls were analysed and compared. PHA and PPD in vitro stimulation significantly increased INF-gamma levels and the cellular expression of IL-12Rb1 and INF-gR1 receptors compared to baseline levels in both TB patients and healthy controls (either with positive or negative TST). Our results suggest that there is an adequate immune response in all groups. Although the IL-12/INF-g axis may have other abnormalities not analysed in this work, the functionality of the IL-12/INF-g axis is preserved in the patients analysed with TB in Galicia. The high rates of disease historically observed in this community does not seem to be caused by a malfunction of the immune response at this level (AU)


Assuntos
Humanos , Tuberculose/imunologia , Interleucina-12/imunologia , Interferon gama/imunologia , Testes de Liberação de Interferon-gama , Imunidade Celular
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