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1.
Acta pediatr. esp ; 76(9/10): 99-104, sept.-oct. 2018. tab
Artigo em Espanhol | IBECS | ID: ibc-177416

RESUMO

La osteopontina (OPN) es una proteína bioactiva cuya ubicuidad y alta variabilidad molecular determinan que asuma funciones muy diversas por todo el organismo, aunque aún no están totalmente establecidas. La OPN está presente en la leche humana, donde es capaz de interaccionar favorablemente con otros componentes de la leche materna, como el calcio, y potenciar la actividad de proteínas como la lactoferrina y la lactoperoxidasa. Sin embargo, también desempeña un papel fundamental en el paso del estado prenatal al posnatal del sistema de respuesta inmunitaria-inflamatoria. La OPN láctea se perfila como un componente bioactivo capaz de emitir señales esenciales para la maduración del sistema inmunitario del lactante y la defensa frente a microorganismos patógenos. Por su similitud estructural y funcional, la adición de OPN de leche bovina a fórmulas infantiles puede promover una actividad fisiológica no nutritiva beneficiosa para el lactante. Se espera disponer de nuevos estudios que consoliden la evidencia y proporcionen datos cuantitativos de estos beneficios


Osteopontin (OPN) is a bioactive protein, whose ubiquity and high molecular variability makes it assume very different functions throughout the body, although they are not yet fully established. Milk OPN is present in human milk and is capable to interact favorably with other breast milk components as calcium, and enhance the activity of proteins such as lactoferrin and lactoperoxidase. But it plays a key role in the change from prenatal to postnatal state of the immune-inflammatory system response. Milk OPN is shaped up as a bioactive component able to send out essential signals for the maturation of the infant’s immune system and the defense against pathogenic organisms. Since their structural and functional similarity, the addition of bovine milk OPN to infant formulas could promote a non-nutritive physiologic activity beneficial to the infant. New studies are expected in order to gain solid evidence and provide quantitative data of these benefits


Assuntos
Humanos , Animais , Lactente , Pré-Escolar , Camundongos , Osteopontina/uso terapêutico , Fórmulas Infantis , Sistema Imunitário , Nutrição do Lactente , Saúde da Criança , Nutrientes , Medicina Baseada em Evidências
2.
Allergol. immunopatol ; 46(2): 144-118, mar.-abr. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-172172

RESUMO

Background: Allergic conjunctivitis (AC) is one of the most common allergic ocular diseases worldwide. Osteopontin (OPN), as a recently described Th2 inflammation related protein, may play a role in the pathogenesis of AC. The aim of this study was to identify the expression of OPN in children with AC. Methods: Eighty AC children (seasonal and perennial AC) and twenty controls were enrolled in this study. Serum and tears of different time points (during and out of the pollen season) were collected and used for enzyme-linked immunosorbent assay (ELISA) of OPN and T-help cell related cytokines, respectively. The relationship between serum and tears OPN and Th1/2/17Treg related cytokines as well as disease severity were analysed. Results: Our results showed that expression of tear OPN protein by perennial AC patients increased significantly compared with controls or seasonal AC patients out of the pollen season. Tear OPN expression was positively related to local Th2/17 cytokines and negatively related to IL-10 and TGF-Beta expression. The tear OPN expression was also significantly related to disease severity. Conclusion: Tear OPN reflects the local clinical status of ocular allergy and might play an important pathophysiological role in local Th2/17/Treg inflammation in children with AC (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Conjuntivite Alérgica/imunologia , Lágrimas/imunologia , Osteopontina/imunologia , Citocinas/imunologia , Células Th1/imunologia , Células Th17/imunologia , Células Th2/imunologia , Ensaio de Imunoadsorção Enzimática/métodos
3.
Allergol. immunopatol ; 45(6): 534-540, nov.-dic. 2017. tab
Artigo em Inglês | IBECS | ID: ibc-168460

RESUMO

Background: It is thought that airway inflammation is more common in obese asthmatic patients because inflammation is harder to control and does not respond well to glucocorticoid treatment. Objective: This study's aim was to investigate the effect of obesity on airway and systemic inflammation in children with asthma and to identify the biomarkers that play a role in this inflammation. Methods: The study included patients aged 6-16 years who were diagnosed with asthma in the paediatric allergy outpatient clinic of Bagcilar Training and Research Hospital in Turkey. Complete blood count parameters were compared between three groups: obese asthmatic (n = 43), obese non-asthmatic (n = 45), and non-obese non-asthmatic (control group, n = 30). Levels of high-sensitive CRP (hs-CRP), neutrophil gelatinase-associated lipocalin (NGAL), osteopontin (OPN), and matrix metalloproteinase-9 (MMP-9), and 25(OH)-vitamin D were compared between the groups. Results: No statistically significant differences were observed in 25(OH)-vitamin D, NGAL, OPN, hs-CRP, and MMP-9 levels between groups. There was a statistically significant negative correlation between FEV1/FVC and NGAL and MMP-9. Conclusion: This is the first study to investigate levels of hs-CRP, NGAL, OPN, MMP-9, and 25(OH)-vitamin D in obese asthmatic children. Larger studies with sputum and BAL examinations are required to determine the potential of biomarkers for identifying inflammation in obese asthmatic children (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Inflamação/fisiopatologia , Asma/fisiopatologia , Obesidade/fisiopatologia , Biomarcadores/análise , Mediadores da Inflamação/análise , Osteopontina/análise , Lipocalina-2/análise , Metaloproteinase 9 da Matriz/análise , Líquido da Lavagem Broncoalveolar/citologia , Doença Pulmonar Obstrutiva Crônica/fisiopatologia
4.
Angiología ; 68(6): 459-464, nov.-dic. 2016. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-157708

RESUMO

Introducción: La osteopontina (OPN) incrementa el reclutamiento, migración y adhesión de los macrófagos y modula la expresión de citocinas proinflamatorias e interleucinas. Actualmente, no está definida su asociación con la inestabilidad de la placa de ateroma carotídea y la sintomatología clínica de los pacientes. Objetivos: Estudiar los niveles en plasma de OPN de pacientes intervenidos quirúrgicamente de endarterectomía carotídea (ECA) y correlacionarlos con la sintomatología clínica preoperatoria, con el fin de valorar el riesgo neurológico y la inestabilidad de placa. Material y métodos: Se diseñó un estudio prospectivo con una muestra de pacientes consecutivos intervenidos quirúrgicamente de ECA, previamente evaluados por el neurólogo o con la realización de una TAC o RMN cerebral. Los pacientes se dividieron en 2 grupos (sintomáticos y asintomáticos) y se compararon con un grupo control. Se excluyeron aquellos con enfermedades intercurrentes. La OPN se determinó mediante enzimoinmunoanálisis. Se utilizó para el análisis estadístico el programa SPSS v. 18.0. Las variables categóricas se describen como frecuencias y las cuantitativas como media y desviación estándar en el caso de utilizar pruebas paramétricas, y como mediana y rango intercuartil en el caso de utilizar pruebas no paramétricas. Se estableció que la relación fue estadísticamente significativa si p era inferior a 0,05. Resultados: Durante el periodo de estudio, 44 pacientes (39 hombres, 5 mujeres), de edad media 75±6,62 años, fueron intervenidos de ECA por presentar una placa de ateroma que producía una estenosis significativa (>70% con ecodoppler). De acuerdo con sus antecedentes cerebrovasculares, 24 fueron sintomáticos y 20 asintomáticos. El grupo control fue de 25 sujetos sanos. La OPN en el grupo control fue de 60±6,62 ng/mL, de 74,3±60,8 ng/mL en asintomáticos y de 90,3±45,4 ng/mL en sintomáticos (p = 0,003). Conclusiones: La (OPN) se comporta como un buen indicador de riesgo cerebrovascular en pacientes con placa carotídea, a pesar de que sus niveles y los mecanismos moleculares de expresión no están del todo aclarados (AU)


Introduction: Osteopontin (OPN) increases recruitment, migration and adhesion of macrophages and modulates the expression of proinflammatory cytokines and interleukins. Its association with the instability of carotid atheromatous plaque and the clinical symptoms of patients is currently not defined. Aims: To study plasma levels of OPN in patients operated on by carotid endarterectomy (ECA) and correlate them with preoperative clinical symptoms, in order to assess the neurological risk and instability of plaque. Material and methods: A prospective study was designed with a sample of consecutive patients who underwent surgery by ECA, and previously evaluated by a neurologist and/or by performing a CT or MRI brain scan. Patients were divided into 2 groups (symptomatic and asymptomatic) and compared with a control group. Those with intercurrent diseases were exclude. OPN was determined by enzyme immunoassay. Windows SPSS v. 18.0 program was used for statistical analysis. Categorical variables are described as frequencies, and quantitative variables as mean and standard deviation, in the case of using parametric tests, and median and interquartile range when using non-parametric tests. A P<.05 was established as a statistically significant relationship. Results: During the study period, 44 patients (39 males, 5 females), mean age 75±6.62 years old, were operated on by ECA due to having a plaque that produced a significant stenosis (> 70% with echodoppler). According to their stroke history, 24 were symptomatic and 20 asymptomatic. The control group was 25 healthy subjects. The OPN in the control group was 60±6.62 ng/mL, 74.3±60.8 ng/mL in asymptomatic, and 45.4±90.3 ng/mL in symptomatic patients (P=.003). Conclusions: OPN behaves as a good indicator of stroke risk in patients with carotid plaque, even though its levels and molecular mechanisms of expression are not entirely clear (AU)


Assuntos
Humanos , Masculino , Feminino , Osteopontina/administração & dosagem , Osteopontina/metabolismo , Doenças das Artérias Carótidas/patologia , Estenose das Carótidas/congênito , Citocinas/administração & dosagem , Arteriosclerose/congênito , Arteriosclerose/patologia , Calcificação Vascular/sangue , Osteopontina/provisão & distribução , Osteopontina/uso terapêutico , Doenças das Artérias Carótidas/metabolismo , Estenose das Carótidas/complicações , Citocinas/metabolismo , Estudos Prospectivos , Arteriosclerose/metabolismo , Calcificação Vascular/patologia
5.
Rev. esp. med. nucl. imagen mol. (Ed. impr.) ; 35(2): 102-106, mar.-abr. 2016. tab, graf
Artigo em Inglês | IBECS | ID: ibc-148916

RESUMO

Objective. In this study, an evaluation was made of the relationship between the serum levels of carcinoembryonic antigen (CEA), osteopontin (OPN), and the semi-quantitative parameters of 18-fluoro-2-deoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) in lung cancer patients with bone metastasis. Material and methods. The evaluation included 42 non-small cell lung cancer (NSCLC) and 31 small cell lung cancer (SCLC) patients who were referred to our institution for staging by 18F-FDG PET/CT. The biochemical parameters measured included CEA and OPN serum levels. Results. Serum levels of OPN in NSCLC patients with and without bone metastasis were 21.20 ± 4.97 ng/ml and 13.33 ± 4.53 ng/ml, respectively (p < 0.05). In SCLC patients with and without bone metastasis serum OPN levels were 23.95 ± 4.78 ng/ml and 17.30 ± 3.09 ng/ml, respectively (p < 0.05). Serum levels of CEA in NSCLC patients with and without bone metastasis were 33.79 ± 6.49 ng/ml and 11.74 ± 2.96 ng/ml, respectively (p < 0.05). In SCLC patients with and without bone metastasis serum levels of CEA were 28.93 ± 4.59 ng/ml and 13.88 ± 4.47 ng/ml, respectively (p < 0.05). There were no correlations between primary tumor SUVmax, and serum levels of CEA and OPN. Conclusions. Bone metastasis can be detected in patients with lung cancer by measuring CEA and OPN levels. Increased levels of CEA and OPN levels may be considered an early warning sign in patients needing accurate imaging, as they are at higher risk of bone metastasis (AU)


Objetivo. Evaluar la relación entre los niveles de antígeno carcinoembriionario (CEA), osteopontina (OPN) y los valores semicuantitativos (SUV) de la PET/TC con 18F-FDG en pacientes con metástasis óseas por cáncer de pulmón. Material y método. Se incluyeron 40 pacientes con cáncer de pulmón de células no pequeñas (NSCLC) y 31 pacientes con cáncer de pulmón de células pequeñas (SCLC) referidos a nuestro centro para la realización de un estudio PET/TC con 18F-FDG de estadificación. Se analizarón los niveles sanguíneos de OPN y CEA. Resultados. Los niveles de OPN en pacientes con NSCLC con y sin metástasis óseas fueron de 21.20 ± 4.97 ng/ml y 13.33 ± 4.53 ng/ml, respectivamente (p < 0.05). En pacientes con SCLC con y sin metástasis óseas fueron de 23.95 ± 4.78 ng/ml y 17.30 ± 3.09 ng/ml, respectivamente (p < 0.05). Los niveles sanguíneos de CEA en pacientes de NSCLC con y sin metástasis óseas fueron de 33.79 ± 6.49 ng/ml y 11.74 ± 2.96 ng/ml, respectivamente (p < 0.05). En pacientes con SCLC con y sin metástasis óseas fueron de 28.93 ± 4.59 ng/ml y 13.88 ± 4.47 ng/ml, respectivamente (p < 0.05). No hubo correlación entre el SUV máximo del tumor primario, los niveles OPN ni de CEA. Conclusiones. La metástasis ósea puede ser detectada en pacientes con cáncer de pulmón con la determinación de los niveles de OPN y CEA. Los niveles incrementados de CEA y OPN pueden ser considerados como una señal de advertencia temprana en pacientes que necesiten imágenes precisas, porque ellos están en mayor riesgo de metástasis en el hueso (AU)


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Carcinoma Pulmonar de Células não Pequenas/sangue , Carcinoma Pulmonar de Células não Pequenas/complicações , Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares , Antígeno Carcinoembrionário/análise , Osteopontina/análise , Osteopontina , Metástase Neoplásica/patologia , Metástase Neoplásica , Tomografia por Emissão de Pósitrons/métodos , Tomografia por Emissão de Pósitrons , Sorologia/tendências
6.
Allergol. immunopatol ; 42(4): 275-281, jul.-ago. 2014. tab, graf
Artigo em Inglês | IBECS | ID: ibc-125195

RESUMO

Background: The role of osteopontin (OPN) has not been elucidated in childhood asthma. Objective: Our purpose was to investigate whether OPN levels change due to allergic inflammation in pre-school and school-age children. Methods: In this prospective, cross-sectional study, 42 healthy children and a total of 51 children with asthma were recruited. OPN levels and its association with clinical and laboratory parameters were investigated in the study population. The asthma group were divided into two groups with respect to age, ≤5-years (n = 23) and >5-years (n = 28), and labelled Asthma Group 1 and Asthma Group 2, respectively. OPN levels were compared between subgroups. Results: Serum OPN levels were significantly higher in the asthma group when compared to the control group (p = 0.004). OPN levels were similar in Asthma Group 1 and control groups, whereas it was found to be higher in Asthma Group 2 (p > 0.025, p = 0.001, respectively). In the >5-years age asthmatic group, OPN levels of the patients with allergic rhinitis (n = 15) were higher than those of the patients (n = 13) without allergic rhinitis (p = 0.021). Conclusion: The study underscores the relationship between childhood asthma and OPN as the first study in the literature. In this study we found that OPN, which plays a role in Th2 mediated inflammation, may also play a role in childhood asthma. The fact that OPN levels do not increase in preschool-age children with asthma might be due to the transient wheezing in this group (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Osteopontina/sangue , Asma/sangue , Sons Respiratórios/imunologia , Estudos Prospectivos , Estudos de Casos e Controles , Células Th2 , Mediadores da Inflamação/análise , Inflamação/imunologia
7.
Med. oral patol. oral cir. bucal (Internet) ; 18(4): 657-663, jul. 2013. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-114488

RESUMO

Objectives: To assess the effect of topical application of melatonin to the gingiva on salivary fluid concentrations of acid phosphatase, alkaline phosphatase, osteopontin, and osteocalcin. Study Design: Cross-sectional study of 30 patients with diabetes and periodontal disease and 30 healthy subjects. Diabetic patients were treated with topical application of melatonin (1% orabase cream formula) once daily for 20 days and controls with a placebo formulation. Results: Before treatment with melatonin, diabetic patients showed significantly higher mean salivary levels of alkaline and acid phosphatase, osteopontin and osteocalcin than healthy subjects (P < 0.01). After treatment with melatonin, there was a statistically significant decrease of the gingival index (15.84± 10.3 vs 5.6 ± 5.1) and pocket depth (28.3 ± 19.5 vs 11.9 ± 9.0) (P < 0.001). Also, use of melatonin was associated with a significant reduction of the four biomarkers. Changes of salivary acid phosphatase and osteopontin correlated significantly with changes in the gingival index, whereas changes of alkaline phosphatase and osteopontin correlated significantly with changes in the pocket depth. Conclusions: Treatment with topical melatonin was associated with an improvement in the gingival index and pocket depth, a reduction in salivary concentrations of acid phosphatase, alkaline phosphatase, osteopontin and osteocalcin (AU)


Assuntos
Humanos , Diabetes Mellitus/epidemiologia , Doenças Periodontais/epidemiologia , Melatonina/farmacocinética , Fosfatase Ácida , Fosfatase Alcalina , Gengivite/tratamento farmacológico , Doenças da Gengiva/tratamento farmacológico , Osteopontina , Osteocalcina
8.
Clin. transl. oncol. (Print) ; 15(1): 65-71, ene. 2013. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-126969

RESUMO

AIM AND BACKGROUND: Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase involved in many important aspects of cell biology that are related to tumorigenesis. There are opposite evidences of the role of EGFR in renal cancer and the outcome of EGFR-targeted therapies, suggesting the complexity of EGFR signaling pathways. In vitro, osteopontin (OPN) and nuclear factor kappa B (NF-κB) are thought to be involved in specific ligand-independent EGFR activation that could have a role in resistance to EGFR mAb therapy. Aim of this study was to analyze the relationship between EGFR and OPN at the protein and mRNA level, as well as their relation to NF-κB in clear cell renal cell carcinoma (CCRCC). MATERIALS AND METHODS: Expression of EGFR, OPN, and p65 NF-κB protein was analyzed using immunohistochemistry and compared mutually in 88 CCRCC samples. Expression of EGFR and OPN mRNAs was analyzed using quantitative Real-time PCR in 22 CCRCC samples and compared mutually and with NF-κB protein expression. RESULTS: Epidermal growth factor receptor mRNA level was higher in CCRCC samples in comparison with normal renal tissue (p = 0.012) and was associated with high OPN mRNA level, and with NF-κB activation (p < 0.001 and p = 0.045, respectively). Immunohistochemical staining showed the inverse association; high EGFR protein expression was related with low OPN and NF-κB protein expression (p < 0.001 and p = 0.047, respectively). CONCLUSION: Epidermal growth factor receptor gene is upregulated in CRCC and associated with OPN gene expression and NF-kB signaling. The inverse relation between OPN and EGFR at the protein level could probably reflect dynamic changes that EGFR undergoes following activation (AU)


Assuntos
Humanos , Carcinoma de Células Renais/genética , Carcinoma de Células Renais/metabolismo , Carcinoma de Células Renais/patologia , Linhagem Celular Tumoral , Imuno-Histoquímica , NF-kappa B/genética , NF-kappa B/metabolismo , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Neoplasias Renais/genética , Neoplasias Renais/metabolismo , Neoplasias Renais/patologia , Osteopontina/genética , Osteopontina/metabolismo , Receptores ErbB/genética , Receptores ErbB/metabolismo
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