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1.
J. physiol. biochem ; 74(2): 207-221, mayo 2018. graf, tab
Artigo em Inglês | IBECS | ID: ibc-178978

RESUMO

The dissociated dorsal root ganglion (DRG) neurons with or without culture were widely used for investigation of their electrophysiological properties. The culture procedures, however, may alter the properties of these neurons and the effects are not clear. In the present study, we recorded the action potentials (AP) and the voltage-gated Na+, K+, and Ca2+ currents with patch clamp technique and measured the mRNA of Nav1.6-1.9 and Cav2.1-2.2 with real-time PCR technique from acutely dissociated and 1-day (1-d) cultured DRG neurons. The effects of the nerve growth factor (NGF) on the expression of Nav1.6-1.9 and Cav2.1-2.2 were evaluated. The neurons were classified as small (DRG-S), medium (DRG-M), and large (DRG-L), according to their size frequency distribution pattern. We found 1-d culture increased the AP size but reduced the excitability, and reduced the voltage-gated Na+ and Ca2+ currents and their corresponding mRNA expression in all types of neurons. The lack of NGF in the culture medium may contribute to the reduced Na+ and Ca2+ current, as the application of NGF recovered some of the reduced transcripts (Nav1.9, Cav2.1, and Cav2.2). 1-d culture showed neuron-type specific effects on some of the AP properties: it increased the maximum AP depolarizing rate (MDR) and hyperpolarized the resting membrane potential (RP) in DRG-M and DRG-L neurons, but slowed the maximum AP repolarizing rate (MRR) in DRG-S neurons. In conclusion, the 1-d cultured neurons had different properties with those of the acutely dissociated neurons, and lack of NGF may contribute to some of these differences


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Assuntos
Animais , Feminino , Ratos , Gânglios Espinais/citologia , Gânglios Espinais/fisiologia , Neurônios/fisiologia , Potenciais de Ação , Canais de Cálcio/genética , Canais de Cálcio/fisiologia , Células Cultivadas , Fator de Crescimento Neural , Técnicas de Patch-Clamp , Canais de Potássio de Abertura Dependente da Tensão da Membrana/genética , Canais de Potássio de Abertura Dependente da Tensão da Membrana/fisiologia , RNA Mensageiro/genética , Ratos Sprague-Dawley
2.
Med. clín (Ed. impr.) ; 133(6): 224-228, jul. 2009. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-73239

RESUMO

Fundamento y objetivo: La encefalitis límbica (EL) asociada a anticuerpos contra canales de potasio dependientes de voltaje (Ac-CKDV) es una entidad de reciente descripción, en general no asociada a cáncer y con buena respuesta al tratamiento inmunodepresor. Describimos las características clínicas, radiológicas y el perfil evolutivo de una serie de pacientes en nuestro medio. Pacientes y método: Análisis retrospectivo de variables demográficas, clínicas y evolutivas de una serie de pacientes con EL con Ac-CKDV y ausencia de anticuerpos onconeuronales. Resultados: En un período de 10 años se identificaron 13 pacientes; el 77% fueron varones y la mediana de edad de 67 años (extremos 50 75). La mediana de presentación fue de 10 semanas (extremos 1 24 semanas). Todos presentaron alteración de la memoria episódica, un 77% crisis epilépticas y un 77% síndrome confusional o alteración de la conducta. Un trastorno de conducta sueño-REM (TCSR) se detectó en 9 pacientes interrogados. La resonancia magnética (RM) fue anormal en el 84,6%, el electroencefalograma (EEG) en el 91%, el líquido cefalorraquídeo (LCR) en el 60% y se detectó hiponatremia en el 83% de los casos. Todos los pacientes recibieron tratamiento con inmunoglobulinas y/o glucocorticoides y el 61,5% quedó asintomático o con síntomas mínimos. Tras un seguimiento mediano de 24 meses (extremos 3 108 meses) ningún paciente desarrolló cáncer. Conclusiones: Las características demográficas y clínicas de nuestra serie son similares a las ya publicadas y se confirma que se trata de una entidad con buena respuesta al tratamiento. La presentación crónica y la normalidad de la RM no descarta el diagnóstico sindrómico y en estos casos la existencia de un TCSR, hiponatremia o un LCR inflamatorio resultan de especial ayuda diagnóstica (AU)


Background and objective: Limbic encephalitis (LE) associated with antibodies to voltage-gated potassium channels (VGKC-Ab) have recently been reported as an immunotherapy responsive encephalitis typically not associated with cancer. In the present study, we report the clinical, radiological and evolution features of LE associated with VGKC-Ab in our area. Patients and method: Retrospective analysis of clinical, radiological and evolution variables in a series of LE associated with VGKC-Ab without onconeuronal antibodies. Results: Thirteen patients were detected during a 10-years period; 77% were male and the median age was 67 years (50 75 years). The median time of presentation was 10 weeks (1 24 weeks). All patients had episodic memory impairment, 77% had epileptic seizures and 77% had confusion or behaviour disturbances. A REM-behaviour disorder (RBD) was detected in all asked patients. MRI was abnormal in the 84.6%, EEG in the 91%, CSF in 60% and hyponatraemia was detected in the 83% of cases. All cases were treated with IGIV and/or costicosteroids and 61.5% remained asymptomatic or with minimal symptoms. After a median follow-up of 24 months (3 108 months) no patient developed cancer. Conclusions: Demographic and clinical characteristics of our series are similar to those previously reported, confirming its immunotherapy-responsive condition. Chronic presentation or normal MRI does not exclude the syndromic diagnoses, and the coexistence of RBD, hyponatraemia or inflammatory CSF are especially useful to make the diagnoses in these cases (AU)


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Encefalite Límbica/imunologia , Canais de Potássio de Abertura Dependente da Tensão da Membrana/imunologia , Encefalite Límbica/tratamento farmacológico , Estudos Retrospectivos , Imunoglobulinas/uso terapêutico , Glucocorticoides/uso terapêutico , Evolução Clínica , Encefalite Límbica
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