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1.
Endocrinol. diabetes nutr. (Ed. impr.) ; 67(3): 186-193, mar. 2020. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-188147

RESUMO

Introducción: Evaluar la seguridad y eficacia de un extracto de aceite de rosa mosqueta en la prevención y tratamiento de las lesiones cutáneas en las manos de los pacientes con diabetes mellitus tipo 1 (DMT1) secundarias a las punciones digitales para el control glucémico. Pacientes y métodos: Estudio prospectivo, aleatorizado, controlado, abierto, con evaluadores ciegos e intervencionista en pacientes de edades entre 6 y 17 años con DMT1 y control intensivo de la glucemia con ≥ 7 punciones capilares diarias durante 12 días. Se evaluaron 3 variables principales (eritema, engrosamiento cutáneo, pérdida de la integridad cutánea) de la siguiente forma: 0: ausente, 1: leve, 2: moderado, 3: intenso. El estudio fue aprobado por el Comité Ético del hospital. Resultados: Se incluyó a 68 niños, por tanto, 136 manos: 80 recibieron aceite de rosa mosqueta y 56 fueron controles. Las características basales de los 2 grupos fueron similares. El 76,3% y el 78,6% presentaban alguna lesión dermatológica inicial, respectivamente. La mediana de valoración global final fue de 0,10 (0,03; 0,30) y de 0,06 (0,00; 0,23), en el grupo de aceite de rosa mosqueta y grupo control, respectivamente. Se encontró una mejoría estadísticamente significativa de la valoración global solo en el grupo control (p = 0,049). No se encontraron diferencias estadísticamente significativas para la comparación de medianas del resto de las variables principales. No se registraron efectos adversos. Conclusión: Se encontró una alta frecuencia de lesiones dermatológicas secundarias a punciones capilares digitales, la mayoría de las cuales fueron lesiones leves. La aplicación de aceite de rosa mosqueta fue segura y no supuso una mejoría en las lesiones dermatológicas


Introduction: This study was intended to assess the efficacy and safety of a rosehip seed oil (RHO) extract in the prevention and treatment of skin lesions in the hands of patients with type 1 diabetes mellitus (T1DM) caused by finger prick blood glucose monitoring. Patients and method: A prospective, randomized, controlled, open-label, rater-blinded trial in patients aged 6-17 years with T1DM and intensive blood glucose control (≥ 7 finger pricks daily) for 12 days. Three main variables (erythema, skin thickening, and loss of skin integrity) were assessed using a scale ranging from 0 (absent) to 3 (severe involvement). The study was approved by the ethics committee of the hospital. Results: Sixty-eight children, and thus 136 hands, were included; 80 hands received rosehip seed oil and 56 hands acted as controls. Baseline characteristics of both groups were similar, with 76.3% and 78.6% of the hands respectively showing skin lesions at study start. Median final global assessment was 0.10 (0.03; 0.30) in the group that received rosehip seed oil and 0.06 (0.00; 0.23) in the control group. A statistically significant improvement in global assessment was found in the control group (P=0.049). No significant differences were found when the medians of the other main variables were compared. No adverse effects were recorded. Conclusion: A high prevalence of skin lesions secondary to finger prick glucose monitoring, most of them mild lesions, was found at study start. Treatment with rosehip seed oil was safe and was not effective for improving skin lesions


Assuntos
Humanos , Criança , Adolescente , Resultado do Tratamento , Rosa , Capilares/lesões , Traumatismos dos Dedos/terapia , Extratos Vegetais/uso terapêutico , Técnica Clamp de Glucose/métodos , Índice Glicêmico , Diabetes Mellitus Tipo 1/diagnóstico , Estudos Prospectivos , Eritema/terapia , Segurança do Paciente
2.
Nutr. hosp ; 37(1): 46-55, ene.-feb. 2020. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-187573

RESUMO

Introducción: las enfermedades crónicas no transmisibles (ECNT) son la principal causa de muerte en todo el mundo. Los metabolitos secundarios provenientes de fuentes vegetales como Cnidoscolus aconitifolius pueden usarse como coadyuvante en la prevención de las enfermedades relacionadas con el estrés oxidativo y la inflamación, tales como las ECNT. Objetivo: se evaluó la actividad antioxidante y antiinflamatoria in vitro de los compuestos biológicamente activos de extractos de C. aconitifolius. Métodos: se determinó el contenido de fenoles, flavonoides, flavanonas e hidroflavonoles. El potencial antioxidante se determinó con los ensayos de 1,1-difenil-2-picrilhidrazilo (DPPH), ácido 2,2'-azinobis (3-etilbenzotiazolina-6-sulfónico) (ABTS) y la actividad inhibitoria de la enzima convertidora de la angiotensina (ACE). Para la actividad antiinflamatoria se utilizaron la reacción en cadena de la polimerasa (PCR) cuantitativa en tiempo real y el ensayo por inmunoabsorción ligado a enzimas (ELISA) en macrófagos diferenciados de monocitos THP-1 y estimulados con lipopolisacárido (LPS). Resultados: el extracto acuoso registró el mayor contenido de fenoles (70,61  0,07 g/100 g de extracto) y el extracto etanólico registró el mayor contenido de flavonoides (47,76  4,84 g/100 g de extracto), flavanonas y dihidroflavonoles (70,10  7,29 g/100 g de extracto). El extracto acetónico registró la mayor inhibición del radical DPPH (49,85 ± 5,30 %) mientras que el etanólico presentó la mayor inhibición del radical ABTS (41,01 ± 3,81 %). Los extractos etanólico y acuoso inhibieron la ECA. El extracto etanólico tuvo la mayor actividad antiinflamatoria al reducir la expresión génica de TNF- en un 39,78 % y la de IL-6 en un 97,81 %, y su producción en un 46 % y un 48,38 %, respectivamente. Conclusiones: los extractos mostraron in vitro su potencial antioxidante y antiinflamatorio por su contenido en compuestos bioactivos


Introduction: noncommunicable diseases (NCDs) are the main cause of death worldwide. Secondary metabolites from plant sources such as Cnidoscolus aconitifolius may be used as adjuvants in the prevention of diseases related to oxidative stress and inflammation such as NCDs. Objective: the in vitro antioxidant and anti-inflammatory activities associated with biologically active compounds in C. aconitifolius extracts were evaluated. Methods: the contents of phenols, flavonoids, flavonones and hydroflavonoles were determined. The potential antioxidant activity was determined with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, and angiotensin-converting enzyme (ACE) activity. For anti-inflammatory activity quantitative PCR and enzyme-linked immunosorbent assay (ELISA) tests were used in macrophages derived from THP-1 monocytes and stimulated with LPS. Results: the aqueous extract recorded the highest phenolic content (70.61  0.07 g/100 g of extract), and the ethanolic extract registered the highest content in flavonoids (47.76  4.84 g/100 g of extract), flavonones and dihydroflavonoles (70.10  7.29 g/100 g of extract). The acetone extract obtained the highest DPPH inhibition (49.85 ± 5.30 %), while the ethanolic extract showed the highest ABTS inhibition (41.01 ± 3.81 %). The etanolic and aqueous extracts had the highest ACE inhibition. The ethanolic extract had the highest anti-inflammatory activity, decreasing gene expression for TNF- by 39.78 % and for IL-6 by 97.81 %, and their production by 46 % and 48.38 %, respectively, in macrophages stimulated with LPS. Conclusions: these extracts demonstrated in vitro their antioxidant and anti-inflammatory potential due to their content of bioactive compounds


Assuntos
Técnicas In Vitro , Antioxidantes/isolamento & purificação , Jatropha/metabolismo , Inflamação/tratamento farmacológico , Extratos Vegetais/farmacologia , Antioxidantes/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Inflamação/metabolismo , Doença Crônica/prevenção & controle , Fenóis , Flavonoides , Flavanonas , Ensaio de Imunoadsorção Enzimática
3.
Nutr. hosp ; 36(5): 1081-1086, sept.-oct. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-184630

RESUMO

Introduction: Stevia rebaudiana extracts can be used as a sweetener due to their glycoside content: specifically stevioside and rebaudioside. Both compounds have adequate pharmacological characteristics for human consumption. Objective: the aim of this study was to standardize the formulation of marmalades using nopal-pineapple-stevia aqueous extract ratios. Methods: the products were evaluated to determine their physicochemical properties, in vitro inhibition of α-amylase and glycemia in healthy volunteers. Storage study was conducted for 20 days at room temperature 23-30 °C and relative humidity 80-85%. Results: incorporation of stevia significantly modified physicochemical properties like °Brix, color and flow index. After storage, the presence of molds and bacteria were not detected. Sensory evaluation indicated that marmalade with 50% stevia replacement was equally accepted as marmalade with sucrose. Marmalade with 50 and 100% of stevia inhibited 35.89 and 38.50% of the α-amylase activity. After an intake of 30 g, it seems that marmalades with stevia had a significant effect on the glycemia of the volunteers. Conclusions: however, further studies with larger doses of nopal-pineapple-stevia marmalade and consumed for longer in both healthy volunteers and patients with diabetes are needed to achieve results that are more precise


Introducción: los extractos de Stevia rebaudiana pueden ser utilizados como edulcorante debido a su contenido de glucósidos: específicamente esteviósido y rebaudiósido. Ambos compuestos presentan características farmacológicas adecuadas para el consumo humano. Objetivos: el objetivo del presente trabajo fue estandarizar formulaciones de mermeladas con diferentes proporciones de nopal-piña-extracto acuoso de stevia. Métodos: se estudiaron las propiedades fisicoquímicas de las mermeladas, su capacidad de inhibir in vitro a la enzima α-amilasa y la glicemia en voluntarios sanos. Los estudios de vida de anaquel se efectuaron durante 20 días a temperatura ambiente 23-30 °C y humedad relativa 80-85%. Resultados: la incorporación de estevia modificó significativamente los grados Brix, el color y el índice de flujo de las mermeladas. Concluido el estudio de anaquel, no se observó la presencia de hongos o bacterias. La evaluación sensorial indicó que la mermelada con 50% de estevia fue aceptada con el mismo nivel de agrado que la mermelada con sacarosa. Las mermeladas con 50 y 100% de estevia inhibieron la actividad de la α-amilasa con valores de 35.89% y 38.50%, respectivamente. Posterior a una ingesta de 30 g de mermelada se observó un efecto significativo en la glicemia de voluntarios sanos. Conclusiones: deberán efectuarse estudios de consumo prolongado y de mayores cantidades de mermelada tanto en voluntarios sanos como con diabetes para obtener resultados más precisos


Assuntos
Humanos , Geleia de Frutas , Ananas/enzimologia , Físico-Química/métodos , alfa-Amilases/biossíntese , Stevia/química , Ananas/química , Voluntários Saudáveis , Extratos Vegetais/farmacologia , Glicemia/análise , Índice Glicêmico
4.
Allergol. immunopatol ; 47(4): 357-364, jul.-ago. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-186507

RESUMO

Introduction: Amaranthus retroflexus (Redroot Pigweed) is one of the main sources of allergenic pollens in temperate areas. Polcalcin is a well-known panallergen involved in cross-reactivity between different plants. The aim of this study was the molecular cloning and expression of polcalcin, as well as evaluating its IgE-reactivity with A. retroflexus sensitive patients' sera. Methods: Allergenic extract was prepared from A. retroflexus pollen and the IgE-reactivity profile was determined by ELISA and immunoblotting using sera from twenty A. retroflexus sensitive patients. Polcalcin-coding sequence was amplified by conventional PCR method and the product was inserted into pET-21b(+) vector. The recombinant protein was expressed in E. coli BL21 and purified by metal affinity chromatography. The IgE-binding capability of the recombinant protein was analyzed by ELISA and immunoblotting assays, and compared with crude extract. Results: Of 20 skin prick test positive patients, 17 patients were positive in IgE-specific ELISA. Western blotting confirmed that approximately 53% of ELISA positive patients reacted with 10kDa protein in crude extract. The A. retroflexus polcalcin gene, encoding to 80 amino acid residues was cloned and expressed as a soluble protein and designated as Ama r 3. The recombinant polcalcin showed rather identical IgE-reactivity in ELISA and western blotting with 10 kDa protein in crude extract. These results were confirmed by inhibition methods, too. Conclusion: The recombinant form of A. retroflexus polcalcin (Ama r 3) could be easily produced in E. coli in a soluble form and shows rather similar IgE-reactivity with its natural counterpart


No disponible


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Alérgenos/imunologia , Amaranthus/imunologia , Antígenos de Plantas/imunologia , Proteínas de Ligação ao Cálcio/imunologia , Pólen/imunologia , Rinite Alérgica Sazonal/imunologia , Testes Cutâneos , Alérgenos/isolamento & purificação , Antígenos de Plantas/isolamento & purificação , Proteínas de Ligação ao Cálcio/isolamento & purificação , Clonagem Molecular , Reações Cruzadas , Escherichia coli/genética , Expressão Gênica , Imunoglobulina E/metabolismo , Extratos Vegetais , Proteínas Recombinantes/isolamento & purificação
5.
Allergol. immunopatol ; 47(4): 378-385, jul.-ago. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-186510

RESUMO

Introduction and objectives: Allergic asthma is a chronic inflammatory disorder of the airways. Th1, Th2 and Th17 cells are the main cells involved in the pathophysiology of asthma. The function of these cells is affected by T-bet, GATA3 and RORgammat transcription factors (respectively). Therefore, the aim of this study was to evaluate the effect of ginger (officinal Roscoe) extract on the expression of T-bet, GATA-3 and ROR-gamma in peripheral blood mononuclear cells (PBMC) of asthmatic patients, in comparison with healthy volunteers as controls. Materials and methods: In this case-control study, a total of 50 individuals including 25 patients with severe, moderate and mild allergic asthma and 25 unrelated healthy controls were involved. The PBMCs were isolated and divided into four groups: negative control, two positive controls (Budesonide and PHA) and ginger-extract treated group. After cell treatment and incubation for 48h, PBMCs were isolated and cDNA was synthesized. Gene expressions of T-bet, GATA3 and ROR-γt were evaluated by Real-time PCR. Results: According to the results of this study, hydroalcoholic extract of ginger could reduce the expression of GATA-3, ROR-gammat, and T-bet in PBMCs of asthmatic patients in comparison with untreated PBMCs (P values = 0.001, 0.001, and 0.002, respectively). It was also shown that the ginger extract could affect T-bet/GATA-3, T-bet/ROR-gamma, and ROR-gammat/GATA-3 expression ratios. Conclusions: This study showed that the use of ginger extract could control asthma and decrease the severity of this disease by affecting the main cells involving the symptoms of asthma in the airways


No disponible


Assuntos
Humanos , Criança , Adolescente , Adulto Jovem , Adulto , Antiasmáticos/farmacologia , Asma/tratamento farmacológico , Fator de Transcrição GATA3/metabolismo , Hipersensibilidade/tratamento farmacológico , Leucócitos Mononucleares/fisiologia , Extratos Vegetais/farmacologia , Proteínas com Domínio T/metabolismo , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo , Estudos de Casos e Controles , Fator de Transcrição GATA3/genética , Gengibre/imunologia , Regulação da Expressão Gênica , Proteínas com Domínio T/genética
6.
Neurología (Barc., Ed. impr.) ; 34(5): 300-308, jun. 2019. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-180846

RESUMO

Introducción: La acetona cianohidrina (ACH) es una sustancia tóxica resultante de la hidrólisis enzimática de linamarina, contenido en las raíces de yuca (Manihot esculenta Crantz); su consumo a largo plazo se asocia con 2 trastornos neurológicos: konzo y la neuropatía atáxica tropical. Estudios anteriores han evaluado las alteraciones conductuales después del consumo de esta sustancia, pero los efectos tóxicos sobre los procesos fisiológicos se desconocen. Método: Se asignaron 32 ratas Wistar macho a 4 grupos experimentales (n = 8): un grupo vehículo (solución salina 0,3 ml/rata, ip) y 3 grupos con ACH (PubChem CID: 6406) a concentraciones de 10, 15 y 20 mM, durante 28 días, cada 24 h. Se evaluó la actividad motora espontánea en campo abierto y la coordinación motora en pruebas de rotarod y nado a 0, 7, 14, 21 y 28 días de tratamiento. Al final de las pruebas conductuales (día 28) se tomaron muestras de sangre por punción transcardiaca para evaluar la función renal y hepática. Resultados: La ACH promovió alteraciones en la actividad locomotora y promovió tanto el nado lateral como la conducta de giro en la prueba de nado los días 21 y 28 del tratamiento. La ACH incrementó los parámetros de la función renal y hepática de una manera dependiente de la concentración, excepto la glucosa y la bilirrubina total. Conclusión: Estos datos indican que el contenido de este compuesto tóxico contenido en las raíces de yuca podría ser potencialmente peligroso bajo el consumo a largo plazo en sujetos vulnerables


Introduction: Acetone cyanohydrin (ACH) is a toxic substance present in cassava roots (Manihot esculenta Crantz) which results from enzymatic hydrolysis of linamarin. Long-term consumption is associated with 2 neurological disorders: konzo and tropical ataxic neuropathy. Previous studies have evaluated behavioural alterations linked to ACH consumption, but the toxic effects of this substance on physiological processes remain unknown. Method: 32 male Wistar rats were assigned to 4 experimental groups (n = 8 per group): a vehicle group (0.3 mL saline solution, IP) and 3 ACH groups (PubChem CID: 6406) dosed at 10, 15, and 20 mM/24h for 28 days. We evaluated spontaneous motor activity with the open field test and motor coordination with the rotarod and forced swimming tests at 0, 7, 14, 21, and 28 days of treatment. At the end of the assessment period (day 28), blood samples were collected by transcardiac puncture to evaluate kidney and liver function. Results: ACH caused alterations in locomotor activity and promoted both lateral swimming and spinning in the forced swimming test at 21 and 28 days of treatment. Furthermore, it led to an increase in the levels of the parameters of kidney and liver function in a concentration-dependent manner, except for glucose and total bilirubin. Conclusion: Our results suggest that long-term consumption of this toxic compound present in cassava roots may be potentially dangerous for vulnerable subjects


Assuntos
Animais , Ratos , Atividade Motora/efeitos dos fármacos , Fígado/efeitos dos fármacos , Rim/efeitos dos fármacos , Neurotoxinas/isolamento & purificação , Acetona/efeitos adversos , Manihot/efeitos adversos , Ratos Wistar , Extratos Vegetais/farmacocinética , Modelos Animais de Doenças , Testes de Função Renal/estatística & dados numéricos , Testes de Função Hepática/estatística & dados numéricos , Testes de Toxicidade Aguda/métodos
7.
Allergol. immunopatol ; 47(3): 272-276, mayo-jun. 2019. ilus
Artigo em Inglês | IBECS | ID: ibc-186489

RESUMO

Background: Murine models have been widely used in the study of allergy as sensitized mice can produce IgE and/or IgG1in response after the injection of an antigen/adjuvant combination. Ailanthus altissima pollen (AAP) has been recently reported as an emerging aeroallergen in Iran. So far, several AAP candidate allergens by the screening of allergen-specific IgE in the sera from AAP sensitized patients in Iran. Objective: The aim of the present study was to detect and compare the allergens eliciting an IgE response in a mouse model, and in human, using pollen extract of A. altissima and an immunoproteomics based approach. Methods: The pollen proteins were extracted in phosphate-buffered saline (PBS). Thirty male BALB/c mice were randomly divided into two groups of AP extract sensitized and sham that respectively received AAP PBS extract and a PBS control by intraperitoneal injections at regular intervals. The optimized AAP protein extracts were analyzed using 2D-gel electrophoresis and were subsequently confronted to pooled sera of sensitized mice. Results: Two-D gel electrophoresis of AAP extract allowed the separation of 125 protein spots distributed in a wide range of pI and molecular masses. Two-DE immunoblotting using pooled sera of sensitized mice led to the detection of 14 IgE reactive spots with molecular masses ranging from 12 to 40-42kDa. Conclusion: The results do not correlate with our previous analyses using human AAP-sensitized sera. These findings reflect some differences in the sIgE reactivity to allergenic proteins in animal models


No disponible


Assuntos
Humanos , Animais , Masculino , Camundongos , Ailanthus/imunologia , Alérgenos/metabolismo , Antígenos de Plantas/metabolismo , Soros Imunes/metabolismo , Pólen/metabolismo , Rinite Alérgica Sazonal/imunologia , Alérgenos/imunologia , Antígenos de Plantas/imunologia , Modelos Animais de Doenças , Imunoglobulina E/metabolismo , Camundongos Endogâmicos BALB C , Extratos Vegetais , Pólen/imunologia , Eletroforese em Gel Diferencial Bidimensional
8.
Rev. int. androl. (Internet) ; 17(1): 8-14, ene.-mar. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-182209

RESUMO

Objective: Extract of Pygeum africanum (PAE) is commonly used herbal medication in the treatment of benign prostatic hyperplasia. In Montenegro and neighboring countries, PAE is primarily advertised as dietary supplement in the treatment of erectile dysfunction. The purpose of this study was to broaden the current cognition concerning its safety profile. Material and methods: Twenty-four adult male Wistar rats were used. The first control group (O) received water and second control group (OO) received olive oil for 30 days. The third and fourth groups (PA5 and PA10) were treated with PAE dissolved in olive oil (50 and 100mg/kg p.o. daily). The behavior of animals was observed continuously, bodyweight gain (BWG) was calculated weekly and the weight of selected organs was measured at the end of experiment. Total protein and glutathione content of the liver were analyzed. Standard biochemical analyses were also performed. Results: BWG was higher in PA5 compared to both controls at all measuring intervals. Liver weight/body weight ratio was significantly higher in PA10 in comparison with O. Prostate weight/body weight ratio was lower in both PA5 and PA10 compared to OO, achieving statistical significance in PA5. The value of creatinine was higher in PA5 and PA10 compared to both control groups, but achieving statistical significance in PA10 only. LDH was also increased in PA5 and PA10 compared to both controls. Conclusions: Both dosage regimens of PAE, particularly PA10, caused some toxicological effects in Wistar rats after one month of application. Kidney, skeletal muscle and/or myocardium are suspected as target sites of PA toxicity most likely. In order to provide more reliable conclusion it is necessary to conduct an additional research on the basis of these findings


Objetivo: El extracto de Pygeum africanum (PAE) es un producto de origen vegetal que frecuentemente se utiliza en el tratamiento de la hiperplasia benigna de próstata. En Montenegro y en los países limítrofes, el PAE se anuncia principalmente como suplemento dietético en el tratamiento de la disfunción eréctil. El propósito de este estudio fue ampliar el conocimiento actual respecto a su perfil de seguridad. Material y métodos: Se utilizaron 24 ratas macho adultas de raza Wistar. El primer grupo de control (O) recibió agua y el segundo grupo de control (OO) recibió aceite de oliva durante 30 días. El tercer y cuarto grupos (PA5 y PA10) se trataron con PAE disuelto en aceite de oliva (50 y 100mg/kg vo diariamente). Se observó continuamente el comportamiento de los animales, semanalmente se calculó el incremento del peso corporal (IPC), y el peso de los órganos seleccionados se midió al final del experimento. Se analizaron el contenido total de proteína y glutatión del hígado. También se realizaron análisis bioquímicos habituales. Resultados: El IPC fue mayor en PA5 que en los 2 grupos control en todos los intervalos de medición. La relación entre peso del hígado y peso corporal fue considerablemente mayor en PA10 que en O. La relación entre peso de la próstata y peso corporal de la próstata fue menor tanto en PA5 como en PA10 en comparación con OO, por lo que se obtuvo significación estadística en PA5. El valor de la creatinina fue más elevado en PA5 y PA10 en comparación con ambos grupos de control, pero alcanzó significación estadística solo en PA10. La LDH también se incrementó en PA5 y PA10 en comparación con los 2 grupos control. Conclusiones: Las 2 pautas de dosificación de PAE, sobre todo en PA10, provocaron algunos efectos toxicológicos en ratas de raza Wistar después de un mes de aplicación. Se sospecha que muy probablemente el riñón, el musculoesquelético o el miocardio sean lugares diana de los efectos tóxicos de PAE. Para ofrecer una conclusión más fiable, es necesario llevar a cabo más investigación sobre la base de estos hallazgos


Assuntos
Animais , Ratos , Extratos Vegetais/farmacocinética , Hiperplasia Prostática/tratamento farmacológico , Prunus africana/toxicidade , Sistema Musculoesquelético/efeitos dos fármacos , Coração/efeitos dos fármacos , Modelos Animais de Doenças , Estudos de Casos e Controles
9.
Ars pharm ; 60(1): 15-25, ene.-mar. 2019. tab, ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-182796

RESUMO

Objective: Costus pictus D. Don is a traditionally used plant in Naojan area of Golaghat district of Assam, India specifically for treating diabetes. Six compounds were isolated from standardized methanolic extract of the aerial parts (MECP). The prime objective was to select most potent antidiabetic compounds among the isolated compounds viz. F67, F12, F16, F3032, F37 & F48 by using in vitro and in vivo methods. Methods: Isolated compounds were subjected to initial screening by in vitro alfa-amylase inhibition activity assay using iodine-starch and DNSA (3,5-dinitrosalicylic acid) methods. Compounds depicting promising in vitro activity were selected for in vivo Streptozotocin (STZ) induced antidiabetic screening activity. Then based on the in vivo results, most potent compounds were selected for instrumental characterization by Q-TOF ESI-MS, 1HNMR, 13CNMR & FTIR. Results: Amongst the six compounds isolated from MECP, three compounds viz. F12, F16 & F48 showed potent in vitro activity. They were subsequently subjected to evaluation of the antidiabetic activity in vivo by oral administration, at dose of 10, 20 & 50 mg/kg body weight respectively, using Wister rat (120-150 g) and Glibenclamide (10mg/k body weight) as standard. Two compounds, F12 and F48 at dose of 50 mg/kg body weight, reversed STZ induced diabetic parameters (increased blood glucose level, altered plasma profile and histoarchitecture of the pancreatic and hepatic cells) with statistical significance (P<0.05), that was comparable with the standard. Hence, instrumental characterization by Q-TOF ESI-MS, 1HNMR, 13CNMR & FTIR of compounds F12 and F48 isolated from MECP was carried out which established their identity as (3,5,7-Trihydroxy-3'-hydroxy-4'-methoxy) flavanone or [3,5,7-Trihydroxy-2-(3'-hydroxy-4'-methoxy phenyl)-2,3-dihydrochromen-4-one] and 3,5,8-trihydroxy-7-methoxy-2-phenyl-2,3-dihydrochromen-4-one or [7-methoxy-3, 5, 8 trihydroxy flavanone] respectively. Conclusion: The study culminated in elucidation of two flavanones as most potent compounds in exhibiting antidiabetic activities. The findings were thus successful in validating the traditional practices in Golaghat district of Assam, India, associated with the use of Costus pictus D. Don in the treatment of diabetes


Objetivo: Costus pictus D. Don es una planta usada tradicionalmente en la zona del distrito de Golaghat Naojan de Assam, India específicamente para el tratamiento de la diabetes. Seis compuestos se aislaron a partir de extracto metanólico estandarizados de las hojas (MECP). El principal objetivo fue seleccionar compuestos antidiabéticos más potentes entre los compuestos aislados viz. F67, F12, F16, F3032, F37 y F48 mediante métodos in vitro e in vivo. Métodos: Los compuestos aislados fueron sometidos a cribado inicial mediante ensayo de actividad de inhibición in vitro alfa-amilasa utilizando yodo-almidón y métodos DNSA (ácido 3,5-dinitrosalicilico). Los compuestos que presentaban una actividad in vitro prometedora se seleccionaron para la actividad de cribado antidiabético inducida por estreptozotocina (STZ) in vivo. En función de los resultados in vivo, la mayoría de los compuestos potentes se seleccionaron para la caracterización instrumental por Q-TOF ESI-MS, 1HNMR, 13CNMR y FTIR. Resultados: Entre los seis compuestos aislados de MECP, tres compuestos viz. F12, F16 y F48 mostraron una potente actividad. Posteriormente se sometieron a evaluación de la actividad antidiabética in vivo mediante administración oral, en dosis de 10, 20 y 50 mg / kg peso, utilizando ratas Wister (120-150 g) y glibenclamida (10 mg / kg peso) como estándar. Dos compuestos, F12 y F48 en dosis de 50 mg/kg peso, revirtieron los parámetros diabéticos inducidos por STZ (aumento del nivel de glucosa en sangre, plasma perfil alterado y histoarquitectura del páncreas y células hepáticas), con significación estadística (P<0,05) que era comparable con la norma. Se llevo a cabo la caracterización instrumental por Q-TOF ESI-MS, RMN 1H, RMN 13C y FTIR de los compuestos F12 y F48 aislado de MECP, lo que estableció su identidad como (3,5,7-trihidroxi-3'-hidroxi-4'- metoxi) flavanona o [3,5,7-trihidroxi-2- (fenil 3'-hidroxi-4'-metoxi) -2,3-dihydrochromen-4-ona] y 3,5,8-trihidroxi-7-metoxi -2-fenil-2,3-dihydrochromen-4-ona o [7-metoxi-3, 5, 8 trihidroxi flavanona] respectivamente. Conclusión: El estudio culminó en la elucidación de dos flavanonas como los compuestos más potentes en la exposición de actividades antidiabéticas. Los resultados lograron validar las prácticas tradicionales en el distrito de Golaghat de Assam, India, asociados con el uso de Costus pictus D. Don en el tratamiento de la diabetes


Assuntos
Humanos , Feminino , Ratos , Hipoglicemiantes/isolamento & purificação , Hipoglicemiantes/farmacologia , Extratos Vegetais/química , Diabetes Mellitus Experimental/tratamento farmacológico , Costus/química , Hipoglicemiantes/química , Ratos Wistar
10.
Arch. med. deporte ; 36(190): 92-99, mar. 2019. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-186194

RESUMO

Objectives: During maturation period in which the immune system of lung tissue is not fully developed, physical exercises may have a negative effect and cause inflammation. This study aimed to investigate the effects of tapering and Nigella sativa (NS) hydro-alcoholic extract on the reduction of lung tissue inflammation caused due to increasing interval exercise training (IIET ) during maturation period by histological and stereological methods. Methods: Nighty-five three weeks old rats after adaption were randomly divided into two control and exercise groups and 19 subgroups. The exercise group carried out a period of six weeks of undulating IIET followed by three weeks of load reduction performed by three models in two different times. Rats entered the taper phase were administrated by NS supplement in tapering and control groups. The lung tissue samples were processed by standard paraffin embedding, stained by H&E and examined by using point counting method through systematic random sampling in stereological study. The results were analyzed using by two-way ANOVA and LSD post hoc in α=0.05.Results: The result showed that IIET caused severe inflammation in lung tissue and an increase in infiltration of inflammatory cells and lymphocytes into the connective tissues surrounding the respiratory air ways, vessels and interstitial lamellae. This severity of inflammation was considerably and similarly more in comparison to the basic and control groups (p=0.001). Ste-reological analysis in the taper exercise training groups with NS and without NS as well, reveled a significant decrease in the degree and intensity of lung tissue inflammation in the examined times in comparison to the IIET group (p=0.001).Conclusion: Generally it can be concluded that performing NS and a three weeks period of tapering has a noticeable effect in the reduction of inflammation in lung tissue followed by interval exercise training


Objetivos: Durante el período de maduración en el que el sistema inmunitario del tejido pulmonar no está completamente desarrollado, el ejercicio físico puede tener un efecto negativo y causar inflamación. Este estudio tuvo como objetivo investigar los efectos del tapering y del extracto hidroalcohólico de Nigella sativa (NS) sobre la reducción de la inflamación del tejido pulmonar causada por el aumento de entrenamiento interválico (IIET ) durante el período de maduración mediante métodos histológicos y estereológicos.Métodos: Noventa y cinco ratas de tres semanas de edad, después de la adaptación, se dividieron aleatoriamente en dos grupos de control y ejercicio y 19 subgrupos. El grupo de ejercicio llevó a cabo un período de seis semanas de IIET ondulado seguido de tres semanas de tapering realizadas por tres modelos en dos momentos diferentes. Las ratas entraron en la fase de tapering y se les administró un suplemento de NS en ambos grupos. Las muestras de tejido pulmonar se procesaron mediante inclusión convencional de parafina, se tiñeron con H & E y se examinaron mediante el método de conteo puntual mediante muestreo aleatorio sistemático en un estudio estereológico. Los resultados se analizaron usando ANOVA de dos factores y LSD post hoc en α = 0,05Resultados: Los resultados mostraron que el IIET causó inflamación severa en el tejido pulmonar y un aumento en la infil-tración de células inflamatorias y linfocitos en los tejidos conectivos que rodean las vías respiratorias, los vasos y las lamelas intersticiales. Esta gravedad de la inflamación fue considerablemente mayor y similar en comparación con los grupos básico y de control (p = 0,001). El análisis estereológico en los grupos de tapering con NS y sin NS también, reveló una disminución significativa en el grado e intensidad de la inflamación del tejido pulmonar en las mediciones examinados en comparación con el grupo IIET (p = 0,001).Conclusión: en general, se puede concluir que la realización de NS y un período de tapering de tres semanas tiene un efecto notable en la reducción de la inflamación en el tejido pulmonar seguida de entrenamiento de ejercicios a intervalos


Assuntos
Humanos , Masculino , Ratos , Exercício Físico/fisiologia , Pneumonia/prevenção & controle , Pneumonia/fisiopatologia , Nigella sativa/química , Extratos Vegetais/administração & dosagem , Condicionamento Físico Animal/efeitos adversos , Condicionamento Físico Animal/fisiologia , Ratos Wistar , Modelos Animais de Doenças
11.
Gastroenterol. hepatol. (Ed. impr.) ; 42(2): 127-132, feb. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-182108

RESUMO

Aim: This research was conducted to obtain accurate information on the protective effects of Portulaca oleracea L. against hepatogastric diseases. Results: P. oleracea L. (Purslane) has traditionally been used for the treatment of hepatogastric diseases. However, the low number of research studies has shown that P. oleracea L. possesses protective effects against hepatotoxic agents. The safety of P. oleracea L. has been demonstrated in several clinical trials. Conclusion: Modern pharmacological studies have indicated the gastroprotective and hepatoprotective effects of P. oleracea L. by using in vivo and in vitro models. However, due to lack of information of its effects in humans, more studies should be conducted to confirm the efficacy of P. oleracea L. in humans


Objetivo: Se realizó esta investigación para disponer de información precisa sobre los efectos protectores de Portulaca oleracea L. frente a enfermedades hepatogástricas. Resultados: Portulaca oleracea L. (Purslane) se ha utilizado tradicionalmente para el tratamiento de enfermedades hepatogástricas. Sin embargo, las investigaciones limitadas han demostrado que Portulaca oleracea L. posee efectos protectores frente a sustancias hepatotóxicas. Varios ensayos clínicos han demostrado la seguridad de Portulaca oleracea L. Conclusión: Estudios farmacológicos modernos han revelado los efectos gastroprotectores y hepatoprotectores de Portulaca oleracea L. mediante el uso de modelos in vivo e in vitro. Sin embargo, debido a la falta de información sobre sus efectos en el ser humano, se deben realizar más estudios para confirmar la eficacia de Portulaca oleracea L. en el ser humano


Assuntos
Humanos , Animais , Hepatopatias/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Portulaca , Gastropatias/tratamento farmacológico
12.
Allergol. immunopatol ; 47(1): 12-15, ene.-feb. 2019. graf
Artigo em Inglês | IBECS | ID: ibc-180765

RESUMO

Introduction and objectives: Profilin is a panallergen contained in pollen, plant foods and latex. Although cross-reactivity is expected while performing skin prick tests (SPT) with allergens that contain profilin, this is not always noticed. The purpose of this study was to detect if profilin is contained in the commercial SPT extracts of pollen and plant foods which, in their fresh form, contain determined epitopes of profilin. Material and methods: Commercial SPT extracts of different pharmaceuticals were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The study included purified palm date profilin, peach (whole, pulp and peel extracts), hazelnut, Olea europea, Parietaria judaica and Phleum pratense. Results: Profilin was detected in all, but peach extracts; it was neither contained in the whole peach extract nor in the ones of peel or pulp. Conclusion: The only accurate way to detect sensitization to profilin, while performing SPT, is the use of purified profilin extract. Even if a plant food or pollen contain an identified molecule of profilin, the relevant SPT commercial extract may not


No disponible


Assuntos
Humanos , Alérgenos/metabolismo , Antígenos de Plantas/metabolismo , Hipersensibilidade/diagnóstico , Extratos Vegetais/metabolismo , Erros de Diagnóstico/prevenção & controle , Profilinas/metabolismo , Testes Cutâneos/métodos , Alérgenos/imunologia , Antígenos de Plantas/imunologia , Reações Cruzadas , Frutas/imunologia , Olea/imunologia , Parietaria/imunologia , Extratos Vegetais/imunologia , Pólen/imunologia , Profilinas/imunologia , Prunus persica/imunologia
13.
Rev. int. androl. (Internet) ; 16(4): 147-158, oct.-dic. 2018. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-178043

RESUMO

Objective: Prismatomeris memecyloides Craib (Rubiaceae) is a medicinal plant traditionally used by ethnic minorities in Vietnam for the treatment of erectile dysfunction (ED). The aim of this study was to investigate the chemical compositions and screen in silico its possible inhibitory effect against PDE-5 which reduced cyclic guanosine-3′,5′-monophosphate (cGMP) levels and indirectly caused the male ED. Methods: Separation of natural compounds were carried out on chromatographic column with silica gel or reversed phase materials, eluting with different solvent gradients. The structures of all isolated compounds were elucidated on the basis of spectroscopic data (HR-MS, 1D/2D-NMR). Docking simulation study of compound (1-7) was performed by using flexible side chains protocol based on Iterated Local Search Global Optimizer Algorithm of AutoDock/Vina v.1.1.2. Pharmacokinetic parameters and toxicity prediction were also calculated by appropriate softwares. Results: From the methanol extract of roots of P. memecyloides collected in Vietnam, seven compounds including four anthraquinone/one anthraquinone glycoside namely damnacanthal (1), lucidin-ω-methyl ether (2), 3-methylalizarin (3), rubiadin-3-methyl ether (4), and 1-O-methylrubiadin 3-O-primeveroside (5) along with two iridoid glucosides, asperulosidic acid (6) and aitchisonide A (7) were isolated. The molecular modeling results showed that 5 anthraquinone compounds possess the lowest binding energies to PDE-5. The anthraquinone glucoside 1-O-methylrubiadin 3-O-primeveroside (5) potentially inhibited PDE-5 similarly to commercial PDE-5Is sildenafil (SLD) and tadalafil (TLD). Calculated pharmacokinetic results like pIC50,pred; miLogP, TPSA, enzyme inhibitory of anthraquinone glucoside (5) were similar and even higher to those of the commercial PDE-5 inhibitors. Especially the predictive toxicity of 1-O-methylrubiadin 3-O-primeveroside (5) was even lower than those of SLD and TLD. Conclusion: This is the first study to find a scientific-based evidence for the ethnic use of P. memecyloides as medicinal plant for the treatment of ED. The result indicates that the anthraquinones (damnacanthal (1), lucidin-ω-methyl ether (2), 3-methylalizarin (3) and rubiadin-3-methyl ether (4)), especially anthraquinone glycoside (1-O-methylrubiadin 3-O-primeveroside (5)) are compounds of potential novel drug class for the ED treatment


Objetivo: Prismatomeris memecyloides Craib (Rubiaceae) es una planta medicinal utilizada tradicionalmente por las minorías étnicas en Vietnam para el tratamiento de la disfunción eréctil (DE). El objetivo de este estudio fue investigar sus composiciones químicas y realizar un cribado virtual de su posible efecto inhibitorio frente a PDE-5 con niveles reducidos de guanosín-3′,5′-monofosfato cíclico (cGMP) y la causa indirecta de la DE en los varones. Métodos: Se realizó la separación de los componentes naturales en una columna cromatográfica con gel de silicio o materiales de cambio de fase, diluyéndolos con diferentes gradientes de disolventes. Se dilucidaron las estructuras de los componentes aislados sobre la base de los datos espectroscópicos (HR-MS, 1D/2D-NMR). Se realizó el estudio de simulación de acoplamiento del componente (1-7) utilizando el protocolo de cadenas laterales flexibles basado en el algoritmo «Iterated Local Search Global Optimizer» de los parámetros farmacocinéticos AutoDock Vina v.1.1.2., calculándose asímismo la predicción de toxicidad mediante los softwares adecuados. Resultados: A partir de los extractos de metanol de las raíces de P. memecyloides recolectadas en Vietnam se aislaron 7 componentes, incluyendo 4 antraquinonas/un glucósido antraquinona denominado damnacantal (1), lucidín-ω-metil éter (2), 3-metilalizarín (3), rubiadín-3-metil éter (4) y 1-O-metilrubiadín 3-O-primeverosida (5) junto con 2 glucósidos iridoides, ácido asperulosídico (6) andaitquisonida A (7). Los resultados de modelación molecular reflejaron que 5 componentes de antraquinona poseen las menores energías de ligamiento a PDE-5. El glucósido antraquinona 1-O-metilrubiadín 3-O-primeverosida (5) inhibió potencialmente PDE-5 de manera similar a las PDE-5Is comerciales sildenafil (SLD) y tadalafil (TLD). Los resultados farmacocinéticos calculados como pIC50, pred, miLogP, TPSA, enzima inhibidora de antraquinona glucósido, 1-O-metilrubiadín 3-O-primoverosida (5), fueron similares e incluso más altos que los de los inhibidores comerciales de PDE-5. Especialmente, la toxicidad predictiva de (5) fue incluso menor que la de SLD y TLD. Conclusión: Este es el primer estudio que encuentra una evidencia con base científica para el uso étnico de Prismatormeris memecyloides como planta medicinal para el tratamiento de la DE. El resultado indica que las antraquinonas (damnacantal (1), lucidín-ω-metil éter (2), 3-metilalizarín (3) y rubiadín-3-metil éter (4)), y en especial el glucósido antraquinona (1-O-metilrubiadín 3-O-primeverosida (5)) son componentes de clase farmacológica novel potencial para el tratamiento de la DE


Assuntos
Humanos , Masculino , Disfunção Erétil/tratamento farmacológico , Inibidores da Fosfodiesterase 5/farmacocinética , Antraquinonas/farmacocinética , Extratos Vegetais/farmacocinética , Rubiaceae/química , Resultado do Tratamento
14.
Rev. fitoter ; 18(1): 5-20, dic. 2018. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-181222

RESUMO

La dispepsia funcional (DF) y el síndrome del intestino irritable (SII) son las alteraciones gastrointestinales funcionales más frecuentes. Requieren un tratamiento multifactorial ya que su aparición se relaciona con diversas afecciones funcionales, como alteraciones de la motilidad, hipersensibilidad gastrointestinal, inflamación, alteración de la microbiota intestinal, estrés y trastornos psicológicos, entre otros. SWT 5 es una combinación de 9 extractos vegetales con actividades sinérgicas y complementarias que es capaz de modular la motilidad gástrica e intestinal, inhibe la secreción ácida gástrica, tiene actividad antiinflamatoria y protectora de la mucosa, y reduce la hipersensibilidad intestinal.Desde 1990, se ha demostrado una eficacia estadísticamente significativa en 6 ensayos clínicos aleatorizados, a doble ciego y controlados: cinco en DF (4 frente a placebo y uno frende a cisaprida) y uno en SII (frente a placebo). La eficacia en DF y SII también se ha mostrado en estudios observacionales que involucran más de 5000 adultos y 44000 niños tratados con STW 5. La combinación de extractos presenta una excelente tolerabilidad y un buen perfil de seguridad, con una incidencia de efectos adversos muy baja


A dispepsia funcional (DF) e a síndrome do cólon irritável (SCI) são as alterações gastrointestinais funcionais mais frequentes. Requerem um tratamento multifactorial, porque o seu aparecimento está relacionado com vários distúrbios funcionais, tais como alterações de motilidade, hipersensibilidade gastrointestinal, inflamação, microbioma intestinal alterado, stress e distúrbios psicológicos, entre outros. SWT 5 é uma combinação de 9 extractos vegetais com actividades sinérgicas e complementares que podem modular a motilidade gástrica e intestinal, têm actividade inibidora da secreção ácida gástrica, anti-inflamatória e protectora da mucosa, e reduzem a sensibilidade intestinal. Desde 1990, demonstrou-se uma eficácia estatisticamente significativa em seis ensaios clínicos randomizados, duplocego, e controlados: cinco em DF (4 versus placebo e um versus cisaprida) e um em SCI (versus placebo). A eficácia no tratamento de DF e SCI também foi demonstrada atra-vés de estudos observacionais envolvendo mais de 5000 adultos e 44000 crianças tratadas com STW 5. A combinação dos extractos mostra uma excelente tolerabilidade e um bom perfil de segurança, com uma incidência muito baixa de efeitos adversos


Functional dyspepsia (FD) and irritable bowel syndrome (IBS) are the most frequent functional gastrointestinal (GI) disorders. They require a multifactorial treatment since they appear to be related to several functional conditions, such as alterations of motility, hypersensitivity, inflammation, alteration of the gut microbiota, stress and psychological disorders, among others. SWT 5 is a combination of 9 herbal extracts with synergistic and complementary activities that is able to modulate gastric and intestinal motility, inhibits gastric acid secretion, shows mucosal anti-inflammatory and protective activities, and reduces intestinal hypersensitivity.Since 1990, statistically significant efficacy has been shown in 6 randomized, double-blind, controlled clinical trials: five in DF (4 versus placebo and one in front of cisapride) and one in IBS (versus placebo). The efficacy in DF and IBS has also been shown in observational studies involving more than 5000 adults and 44000 children treated with STW 5. The combination of extracts presents an excellent tolerability and a good safety profile, with a very low incidence of adverse effects


Assuntos
Humanos , Dispepsia/tratamento farmacológico , Síndrome do Intestino Irritável/tratamento farmacológico , Medicamentos Fitoterápicos , Azia/tratamento farmacológico , Fitoterapia/métodos , Resultado do Tratamento , Extratos Vegetais/farmacocinética
15.
Rev. fitoter ; 18(1): 53-69, dic. 2018. ilus, tab
Artigo em Português | IBECS | ID: ibc-181225

RESUMO

A pele é a primeira linha de defesa do corpo humano. Quando surge uma ferida há uma descontinuidade celular e anatómica de um tecido e por isso há maior suscetibilidade a infeções e outros tipos de agressões. A cicatrização da ferida é um processo fisiológico complexo e dinâmico, que se pode dividir em quatro fases: Homeostase; Inflamatória; Proliferativa; Remodelação. Os produtos de origem natural, particularmente, vários tipos de extratos vegetais e/ou compostos isolados têm demonstrado grande potencial no tratamento de feridas. Em alguns casos, o tratamento convencional pode não ser suficiente para a cicatrização completa das feridas, por exemplo, devido à resistência a antibióticos. A fitoterapia tem demonstrado um enorme potencial na cicatrização de feridas, quer como adjuvante, quer como alternativa à medicina convencional com eficácia demonstrada em ensaios in vitro e in vivo e em ensaios clínicos. Assim, este artigo tem como objetivo não só abordar o tema das feridas e fatores que condicionam a sua cicatrização, como também fazer uma revisão sobre os benefícios de alguns produtos naturais, como o mel de Leptospermum scoparium e extratos de diversas plantas, nomeadamente, Rosa rubiginosa, Centella asiatica, Aloe vera, Curcuma longa e Matricaria recutita


La piel es la primera línea de defensa del cuerpo humano. Cuando se produce una herida hay una discontinuidad celular y anatómica de un tejido y en consecuencia una mayor susceptibilidad a infecciones y otro tipo de agresiones. La cicatrización de la herida es un proceso fisiológico complejo y dinámico, que se puede dividir en cuatro fases: homeostasis, inflamatoria, proliferativa y de remodelación. Los productos de origen natural, particularmente, varios tipos de extractos vegetales y / o compuestos aislados, han demostrado un gran potencial en el tratamiento de las heridas. En algunos casos, el tratamiento convencional puede no ser suficiente para la cicatrización completa de las heridas, debido por ejemplo a la resistencia a los antibióticos. La fitoterapia ha demostrado un enorme potencial en la cicatrización de heridas, ya sea como adyuvante, o como alternativa a la medicina convencional, con eficacia demostrada en ensayos in vitro e in vivo y en estudios clínicos.Este artículo tiene como objetivo no sólo abordar el tema de las heridas y factores que condicionan su cicatrización, sino también hacer una revisión sobre los beneficios de algunos productos naturales, como la miel de Leptospermum scoparium y extractos de diversas plantas, en particular, Rosa rubiginosa, Centella asiatica, Aloe vera, Curcuma longa y Matricaria recutita


The skin is the first line of defense of the human body. When a wound is formed there is a disruption of the cel-lular and anatomic continuity of a tissue and therefore greater susceptibility to infections and other types of agressions. Wound healing is a complex and dynamic process, which can be divided into four phases: Homeostasis; Inflammatory; Proliferative; Remodeling. Natural products have shown good potential in wound treatment. Also, various types of plant extracts and/or plant isolates have demonstrated good efficacy. Sometimes, the convencional treatment is not enough for complete wound healing, for example due to antibiotic resistance. The phytotherapy has demonstrated enormous potencial in treat-ing wounds either as an adjuvante or as an alternative to conventional medicine with demonstrated efficacy in vitroand in vivo assays and in clinical trial. The purpose of this article is not only to address the issue of wounds and the factors that influence healing but also do a review about natural products as Leptospermum scoparium honey and extracts of many plants such as Rosa rubiginosa, Centella asiatica, Aloe vera, Curcuma longa and Matricaria recutita


Assuntos
Humanos , Cicatrização/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Medicamentos Fitoterápicos , Fitoterapia/métodos , Técnicas de Fechamento de Ferimentos , Centella , Rosa , Aloe , Curcuma , Matricaria
17.
Arch. med. deporte ; 35(188): 358-368, nov.-dic. 2018. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-179823

RESUMO

Introducción: La artrosis de rodilla se manifiesta en una primera fase como una gonalgia leve y produce una disminución de la fuerza muscular, que puede ser valorada objetivamente con la prueba isocinética. Dentro de su tratamiento se debe considerar el ácido hialurónico por vía oral. Objetivo: Valorar la evolución de los parámetros isocinéticos de fuerza muscular en la articulación de la rodilla con gonalgia leve antes y después del consumo de un extracto de cresta de gallo (ECG), rico en ácido hialurónico, por vía oral. Metodología: Ensayo de intervención nutricional, doble ciego, aleatorizado, controlado con placebo y en paralelo con dos grupos de tratamiento: grupo activo con ingesta de un yogur bajo en grasa con 80 mg de ECG rico en ácido hialurónico y grupo control con ingesta de un yogur bajo en grasa sin ECG. Se valoraron los parámetros isocinéticos de pico torque (PT), trabajo total (TT) y potencia media (PM) a las velocidades de 180º/seg y 240º/seg para los movimientos de flexión y extensión de la rodilla. Resultados: Después de 12 semanas del consumo del yogur, los hombres del grupo activo, en comparación con los del grupo control, obtuvieron diferencias estadísticamente significativas (p<0,05) y mejora clínica (>10%) a la velocidad de 180º/seg en el movimiento de extensión en la variable PT (p=0,048) (19,33%), TT (p=0,020) (37,97%) y PM (p=0,029) (47,25%), y en el movimiento de flexión en la variable PT (p=0,007) (25,41%), TT (p=0,014) (42,98%) y PM (p=0,022) (48,90%). Conclusión: La ingesta de un yogur bajo en graso con extracto de cresta de gallo rico en ácido hialurónico mejora la fuerza muscular de la rodilla en hombres con gonalgia leve


Background: Knee osteoarthritis manifests itself in a first phase as a mild gonalgia and produces a decrease in muscle strength, which can be objectively assessed with isokinetic testing. In the treatment of knee osteoarthritis, should be considered the hyaluronic acid orally. Objective: Assess the evolution of isokinetic parameters of muscle strength in the knee joint with mild gonalgia before and after the consumption of RCE, rich in hyaluronic acid, orally. Methodology: Nutritional intervention trial, double-blind, randomized, controlled with placebo and in parallel with two treatment groups: the active group with a low-fat yogurt with 80 mg of roster comb extract (RCE) and the control group with a low-fat yogurt without RCE. The main variables of the study were the peak torque, total work and mean power of the isokinetic valuation at the speed of 180 º/seg and 240 º/seg by the movements of flexion and extension of the knee joint. Results: After 12 weeks of consumption of yogurt, men of the active group, compared with those in the control group, obtained statistically significant differences (p <0.05) and clinical improvement (>10%) at the speed of 180º/seg in the movement extension in the PT variable (p=0.048) (19.33%), TT (p=0.020) (37.97%) and PM (p=0.029) (47.25%), and in the flexion movement in the variable PT (p=0.007) (25.41%), TT (p=0.014) (42.98%) and PM (p=0.022) (48.90%). Conclusions: The intake of a low-fat yogurt with RCE rich in hyaluronic acid improves the muscle strength of the knee in men with mild gonalgia


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Osteoartrite do Joelho/tratamento farmacológico , Celosia/química , Ácido Hialurônico/uso terapêutico , Extratos Vegetais/uso terapêutico , Força Muscular , Medição da Dor , Ácido Hialurônico/isolamento & purificação , Método Duplo-Cego , Resultado do Tratamento , Índice de Gravidade de Doença , Estudos de Casos e Controles
18.
J. physiol. biochem ; 74(4): 539-547, nov. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-179032

RESUMO

The link between obesity-induced systemic inflammation and decreased insulin signalling is well-known. It is also known that peripherally produced inflammatory cytokines can cross the blood-brain barrier, resulting in the release of neurotoxins that can ultimately lead to the demise of central nervous system integrity. A high-mesembrine Sceletium tortuosum extract was recently shown to possess cytoprotective and mild anti-inflammatory properties in monocytes and to target specific p450 enzymes to reduce adrenal glucocorticoid synthesis. This is significant since the aetiology of both obesity and diabetes is linked to inflammation and excess glucocorticoid production. Given the interlinked nature of glucocorticoid action and inflammation, central immunomodulatory effects of two Sceletium tortuosum extracts prepared by different extraction methods were investigated. Human astrocytes were pre-treated for 30 min, before exposure to Escherichia coli lipopolysaccharide for 23.5 h (in the presence of treatment). Cytotoxicity, mitotoxicity and cytokine responses (basally and in response to inflammatory stimulus) were assessed. In addition, total polyphenol content, antioxidant capacity and selected neural enzyme inhibition capacity were assessed for both extracts. The high-mesembrine Sceletium extract exerted cytoprotective and anti-inflammatory effects. In contrast, the high delta7-mesembrenone extract, rich in polyphenols, exhibited potent antioxidant effect, although with relatively higher risk of adverse effects with overdose. We conclude that both Sceletium tortuosum extracts may be employed as either a preventative supplement or complimentary treatment in the context of obesity and diabetes; however, current data also highlights the impact that extraction methods can have on plant product mechanism of action


No disponible


Assuntos
Humanos , Anti-Inflamatórios não Esteroides/farmacologia , Astrócitos , Alcaloides Indólicos/farmacologia , Mesembryanthemum/química , Extratos Vegetais/farmacologia , Acetilcolinesterase/metabolismo , Anti-Inflamatórios não Esteroides/análise , Anti-Inflamatórios não Esteroides/isolamento & purificação , Antioxidantes , Linhagem Celular , Inibidores da Colinesterase , Alcaloides Indólicos/análise , Alcaloides Indólicos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação
19.
J. physiol. biochem ; 74(4): 549-558, nov. 2018. graf
Artigo em Inglês | IBECS | ID: ibc-179033

RESUMO

The modern lifestyle is characterised by various factors that cause accelerating ageing by the upregulation of oxidative stress and inflammation—two processes that are inextricably linked in an endless circle of self-propagation. Inflammation in particular is commonly accepted as aetiological factor in many chronic disease states, such as obesity, diabetes and depression. In terms of disease prevention or treatment, interventions aimed at changing dietary and/or exercise habits have had limited success in practise, mostly due to poor long-term compliance. Furthermore, other primary stimuli responsible for eliciting an oxidative stress or inflammatory response-e.g. psychological stress and anxiety-cannot always be easily addressed. Thus, preventive medicine aimed at countering the oxidative stress and/or inflammatory responses has become of interest. Especially in developing countries, such as South Africa, the option of development of effective strategies from plants warrants further investigation. A brief overview of the most relevant and promising South African plants which have been identified in the context of inflammation, oxidative stress and chronic disease is provided here. In addition, and more specifically, our group and others have shown considerable beneficial effects across many models, after treatment with products derived from grapes. Of particular interest, specific cellular mechanisms have been identified as therapeutic targets of grape-derived polyphenols in the context of inflammation and oxidative stress. The depth of these studies afforded some additional insights, related to methodological considerations pertaining to animal vs. human models in natural product research, which may address the current tendency for generally poor translation of positive animal model results into human in vivo models. The importance of considering individual data vs. group averages in this context is highlighted


No disponible


Assuntos
Humanos , Animais , Senilidade Prematura/prevenção & controle , Suplementos Nutricionais , Modelos Biológicos , Estresse Oxidativo , Fitoterapia , Senilidade Prematura/imunologia , Senilidade Prematura/metabolismo , Anti-Inflamatórios não Esteroides/uso terapêutico , Antioxidantes/uso terapêutico , Etnofarmacologia , Medicina Tradicional , Infiltração de Neutrófilos , Extratos Vegetais/uso terapêutico , Especificidade da Espécie
20.
Eur. j. anat ; 22(6): 449-459, nov. 2018. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-182112

RESUMO

The aim of this work was to elucidate a possible protective role of Rumex vesicarius L. (RV) against malathion (MT)-induced hepatotoxicity in adult male albino rats. Forty-eight adult male albino rats were divided into three groups: Group I (control group), 12 rats, divided into two equal subgroups: subgroup Ia received a single intraperitoneal (ip) dose of ½ ml corn oil. Subgroup Ib received oral RV 200 mg/kg daily. Two rats from each subgroup were sacrificed at one day, seven and 28 days. Group II (MT- treated group) 18 rats, each received a single ip dose of 250 mg/kg MT. They were further divided into three equal subgroups: IIa sacrificed at one day, IIb at seven days and IIc at 28 days. Group III (MT plus RV-treated group) 18 rats, each received a single ip dose of MT plus daily RV orally. They were further divided into three equal subgroups: IIIa sacrificed at one day, IIIb at seven days and IIIc at 28 days. Body (BW) and liver weights were recorded and blood samples were collected for Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP). Livers of rats were processed for light and electron microscopic examination.MT administration induced significant decrease in BW and relative liver weights and significant increase in ALT, AST, and ALP levels in addition to histological alterations. Some hepatocytes showed pyknotic nuclei, rarefied, vacuolated, or dark cytoplasm, fat-like droplets with congestion of blood sinusoids and widened space of Disse, appearance of collagenous bundles, abnormal mitochondria with fusion of cytoplasmic organelles in Electron microscopy. Addition of RV to MT in group III significantly improved BW and relative liver weights, biochemical parameters as well as histological picture. MT administration resulted in marked degeneration in the liver that was ameliorated by with concomitant administration of RV


No disponible


Assuntos
Animais , Masculino , Adulto , Ratos , Rumex , Extratos Vegetais/uso terapêutico , Malation/toxicidade , Extratos Vegetais/farmacologia , Fígado/anatomia & histologia , Fígado/ultraestrutura
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