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1.
Rev. lab. clín ; 12(3): e25-e39, jul.-sept. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-187162

RESUMO

Varios miembros de diferentes asociaciones científicas y expertos de la reproducción han actualizado las recomendaciones de estudio genético e inmunológico en las parejas con disfunción en la reproducción con el fin de mejorar la asistencia sanitaria. El estudio se ha considerado altamente recomendable cuando la prueba diagnóstica es relevante para la toma de decisiones, moderada cuando estas han mostrado un resultado poco consistente y baja, cuando el beneficio de la prueba es incierto. Con la indicación de estas recomendaciones obtendremos una información relevante para el diagnóstico, pronóstico y tratamiento de la pareja con disfunción en la reproducción


In this article several members of diverse scientific associations and reproduction experts from Spain have updated different genetic and immunological procedure recommendations in couples affected by reproductive dysfunction with the goal of providing a set of useful guidelines for the clinic. The laboratory test has been considered as highly recommendable for making clinical decisions when the result of the diagnostic test is relevant, moderately recommendable when the results are of limited evidence because they are inconsistent, and low when the benefit of the test is uncertain. It is expected that these recommendations will provide some useful guidelines for the diagnosis, prognosis and treatment of couples presenting reproductive dysfunction


Assuntos
Humanos , Infertilidade/diagnóstico , Testes Imunológicos/métodos , Testes Genéticos/métodos , Técnicas Reprodutivas/ética , Aborto Habitual/genética , Análise Citogenética/métodos , Fenômenos Reprodutivos Fisiológicos/genética , Fenômenos Reprodutivos Fisiológicos/imunologia , Padrões de Prática Médica , Aconselhamento Genético/organização & administração , Infertilidade Masculina/genética , Doenças Genéticas Inatas/prevenção & controle
3.
Rev. esp. enferm. dig ; 111(2): 101-105, feb. 2019. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-182191

RESUMO

Aim: the number of intestinal IgA+ lymphocytes are decreased in acute liver necrosis and the mechanism remains poorly understood. The purpose of this study was to observe the role of lymphocyte homing and apoptosis associated with decreased intestinal IgA positive lymphocytes in acute liver necrosis. Methods: the acute liver necrosis mouse model and LTßR pre-treatment were used to assess intestinal mucosal addressin cell adhesion molecule-1 (MAdCAM - 1) expression, cell apoptosis, IgA+ cells and secretory immunoglobulin A (SIgA). Results: MAdCAM - 1 mRNA and protein expression decreased significantly in the acute necrosis group; 0.57 ± 0.032 fold vs. baseline (p < 0.05) and 0.45 ± 0.072 fold vs. baseline (p < 0.05), respectively. LTßR pre-treatment could significantly improve the decline of MAdCAM - 1 mRNA and protein expression in the intestinal mucosa (1.83 ± 0.064 fold vs. baseline, p < 0.05 and 1.75 ± 0.046 fold vs. baseline, p < 0.05, respectively) and partially restore the decline in IgA+ lymphocytes and SIgA levels. There were increased rates of enterocyte apoptosis in both the acute liver necrosis and LTßR pre-treatment group; 0.79% vs. control (p < 0.05) and 0.77% vs. control (p < 0.05), respectively). Conclusion: our results suggest that the dysfunction of lymphocyte homing and apoptosis are both involved with decreased intestinal IgA+ lymphocytes in acute liver necrosis. LTßR pre-treatment can partially restore IgA+ cells and SIgA by increasing MAdCAM - 1 expression, rather than inhibiting lymphocyte apoptosis


No disponible


Assuntos
Animais , Camundongos , Necrose Hepática Massiva/fisiopatologia , Depleção Linfocítica/métodos , Imunoglobulina A/isolamento & purificação , Morte Celular/fisiologia , Contagem de Linfócitos/métodos , Mucosa Intestinal/fisiopatologia , Testes Imunológicos/métodos , Modelos Animais de Doenças
7.
Allergol. immunopatol ; 46(2): 181-189, mar.-abr. 2018. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-172178

RESUMO

Background: Eosinophilic esophagitis (EoE) is characterized by esophageal dysfunction and, histologically, by eosinophilic inflammation. There is not a clear etiologic treatment. Biopsies analysis using plant histology methods may show callose and pollen tubes in the esophageal mucosa. Component-resolved diagnosis (CRD) with microarrays could detect possible allergens involved and indicate an elimination diet and allergen immunotherapy (AIT). Methods: One hundred and twenty-nine patients with EoE were tested for environmental and food allergens. CRD, histological and botanical analysis were performed. Clinical scores and endoscopic biopsy were performed every six months for three years. Fifty healthy patients, 50 asthmatics due to pollen, and 53 celiac disease patients were included as comparison groups. CRD-directed AIT was administered in 91 EoE patients and elimination diet in 140 patients (87 EoE and all 53 CD patients). Results: CRD detected allergen hypersensitivity in 87.6% of patients with EoE. The predominant allergens were grass group 1 (55%), lipid transfer proteins (LTP) of peach and mugwort, hazelnuts and walnuts. Callose from pollen tubes was found in 65.6% of biopsies. After CRD-guided elimination diet and/or AIT, 101 (78.3%) EoE patients showed significant clinical improvement (p < 0.017) and 97 (75.2%) were discharged (negative biopsy, no symptoms, no medication) without relapse (AU)


No disponible


Assuntos
Humanos , Esofagite Eosinofílica/imunologia , Rinite Alérgica Sazonal/imunologia , Dessensibilização Imunológica , Biópsia , Tubo Polínico/crescimento & desenvolvimento , Pólen/efeitos adversos , Testes Cutâneos , Testes Imunológicos , Estudos de Casos e Controles , Fluorimunoensaio , Estudos Longitudinais , Asma/imunologia , Doença Celíaca/imunologia
8.
Rev. iberoam. micol ; 35(1): 27-31, ene.-mar. 2018. ilus
Artigo em Inglês | IBECS | ID: ibc-170919

RESUMO

Background. Current methods for the production of Histoplasma capsulatum antigens are problematic in terms of standardization, specificity, stability, repeatability and reproducibility. Aims. In this study, we sought to optimize the methodology for producing H. capsulatum antigens, and to evaluate its applicability. Methods. Antigenic preparations obtained from 12 H. capsulatum isolates were evaluated by double immunodiffusion and immunoblotting assays against homologous and heterologous sera. Results. The evaluated and optimized protocol allowed a more stable production, as well as repeatable, reproducible, with shorter culture time and less costly. By double immunodiffusion and immunoblotting assays, the best pattern of reactivity was observed for antigens obtained with 33 days of culture from the isolates 200 and 406 against the M antigen and for the isolate 200 with 15 days against H antigen. The SDS-PAGE presented antigenic components of molecular masses between 17 and 119kDa. The immunoblotting sensitivity was 95.5% and 100% with histoplasmosis sera from ill patients and sera from H. capsulatum infected but otherwise healthy patients, respectively, to the antigen derived from isolates 200 and 406. Conclusions. We suggest the employment of the antigen from isolate 200, with 15 or 30 days of culture, in the double immunodiffusion and immunoblotting assays due to its good ability to discriminate both sera from patients with histoplasmosis illness and histoplasmosis infection, in addition to its high specificity against heterologous sera (AU)


Antecedentes. Los métodos actuales para la producción de antígenos de Histoplasma capsulatum son problemáticos en términos de estandarización, especificidad, estabilidad, repetitividad y reproducibilidad. Objetivos. En este estudio se buscó optimizar la metodología para la producción de antígenos y evaluar su aplicabilidad. Métodos. Las preparaciones antigénicas obtenidas de 12 cepas de H. capsulatum se evaluaron por doble inmunodifusión en gel de agar e inmunotransferencia frente a sueros homólogos y heterólogos. Resultados. El protocolo evaluado y optimizado permitió mayor sensibilidad en la producción, más estabilidad, repetitividad y reproducibilidad con menos tiempo de cultivo y menor coste. Los mejores patrones de reactividad por inmunodifusión en gel de agar e inmunotransferencia se observaron en el antígeno M obtenido tras 33 días de cultivo de las cepas 200 y 406, y en el antígeno H de la cepa 200 tras 15 días de cultivo. Con la técnica SDS-PAGE se separaron componentes antigénicos de masas moleculares entre 17 y 119kDa. La sensibilidad de la inmunotransferencia fue del 95,5% y del 100% con sueros obtenidos de pacientes con enfermedad y con sueros de pacientes infectados, pero sanos, respectivamente, con antígenos derivados de las cepas 200 y 406. Conclusiones. Sugerimos el empleo del antígeno de la muestra 200, con 15 o 30 días de cultivo, en los ensayos de doble inmunodifusión en gel e inmunotransferencia debido a su buena capacidad para discriminar los sueros de pacientes enfermos de histoplasmosis y los de pacientes portadores sanos, además de su elevada especificidad frente a sueros heterólogos (AU)


Assuntos
Humanos , Histoplasmose/imunologia , Histoplasma/isolamento & purificação , Antígenos de Diferenciação/imunologia , Testes Imunológicos/métodos , Immunoblotting/métodos , Imunodifusão/métodos
9.
J. investig. allergol. clin. immunol ; 28(5): 321-329, 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-174533

RESUMO

Background: Allergic diseases are highly prevalent in industrialized populations. In Spain, children suspected of having an allergic disease are usually referred by their primary care pediatrician to an allergy unit at a general hospital or a children’s hospital. We report data from a subanalysis of the pediatric population in Alergológica 2015. Methods: Data were collected from pediatric patients (age, ≤14 years) consulting an allergist for the first time in 2014 and the first quarter of 2015 in order to determine variations compared with data reported in Alergólogica 2005. Results: Alergológica 2015 included fewer pediatric patients than Alergológica 2005. The study population comprised 481 patients aged ≤14 years from more than 200 centers throughout Spain. Males accounted for 56.5%. Rhinoconjunctivitis was the main reason for consulting an allergist (53.8% vs 46.3% in 2005), followed by asthma (30.2% vs 34.6%), and food allergy (20.0% vs 14.5%). Conclusions: The findings of Alergológica 2015 show a notable increased frequency of allergic rhinitis, drug allergy, and food allergy. The frequency of other allergic conditions remained unchanged, except for asthma, whose frequency decreased, as in adult patients


Antecedentes: Las enfermedades alérgicas son muy prevalentes en las poblaciones industrializadas. En España, los niños con sospecha de enfermedad alérgica son habitualmente derivados por su médico de atención primaria a una unidad de alergia de un hospital general o de un hospital infantil. Se notifican los datos del subanálisis de la población pediátrica de Alergológica 2015. Métodos: Se recogieron los datos de los pacientes pediátricos (edad ≤ 14 años) que acudieron al alergólogo por primera vez en 2014 y el primer trimestre de 2015 con el objetivo de determinar las variaciones con respecto a los datos de Alergológica 2005. Resultados: Alergológica 2015 incluyó menos pacientes pediátricos que Alergológica 2005. La población estudiada comprendía 481 pacientes de edad ≤14 años, de más de 200 centros españoles. Los varones representaron el 56,5%. La rinoconjuntivitis fue el principal motivo de consulta al alergólogo (53,8% frente a 46,3% en 2005), seguida del asma (30,2% frente a 34,6%) y la alergia alimentaria (20,0% frente a 14,5%). Conclusiones: Los hallazgos de Alergológica 2015 muestran un aumento notable de la frecuencia de la rinitis alérgica, la alergia a fármacos y la alergia alimentaria. La frecuencia de otras afecciones alérgicas permaneció inalterada, excepto para el asma, cuya frecuencia disminuyó, al igual que en los pacientes adultos


Assuntos
Humanos , Criança , Adolescente , Hipersensibilidade/epidemiologia , Testes Imunológicos/estatística & dados numéricos , Inquéritos de Morbidade , Hipersensibilidade Respiratória/epidemiologia , Dermatite de Contato/epidemiologia , Hipersensibilidade a Drogas/epidemiologia , Hipersensibilidade Alimentar/epidemiologia , Hipersensibilidade Imediata/epidemiologia , Espanha/epidemiologia , Urticária/epidemiologia , Angioedema/epidemiologia
11.
Rev. esp. sanid. penit ; 20(3): 115-124, 2018. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-179556

RESUMO

Objetivos: Evaluar el impacto de los tests rápidos para tuberculosis (TB) añadidos a los sistemas de cribado y de búsqueda pasiva de casos en la carga de enfermedad de TB en centros penitenciarios con alta incidencia de la misma en Azerbaiyán. Material y métodos: Inclusión retrospectiva de todos los casos nuevos y recurrentes de TB notificados desde el 1 de enero de 2009 hasta el 31 de diciembre de 2015. Resultados: Se identificaron un total de 2315 pacientes en 19 centros penitenciarios diferentes. Se implementaron los tests rápidos a los algoritmos de identificación de casos con la consiguiente reducción en las tasas anuales de los casos de TB notificados, los casos de baciloscopia positiva y los de TB resistente a la rifampicina (a una tercera, décima y quinta parte respectivamente). Tras la introducción de los tests rápidos en los algoritmos de cribado se observó una tendencia lineal significativa a la reducción de los casos notificados (p=0,009), los casos de baciloscopia positiva (p=0,011) y los de TB resistente a la rifampicina (p=0,02) con tasas anuales de reducción (con intervalo de confianza al 95%) de -432 (-614;-255), -356 (-517; -195) y -99 (-160;-38) respectivamente. La utilización de los tests rápidos también se tradujo en un incremento significativo del éxito terapéutico con fármacos de primera línea en todos los casos: los detectados por cribado y los identificados por búsqueda pasiva [odds ratio ajustado (Ora)= 2.38, IC95% 1,86-3,05; ORa=4,56, IC 95%:2,64-7,89 y ORa=2,60, IC 95% :1,81-3,75, respectivamente]. Conclusiones: La introducción de los tests rápidos en los sistemas de cribado de TB conllevan reducciones en la carga de enfermedad y en la resistencia a rifampicina con mejores resultados terapéuticos con fármacos de primera línea en centros penitenciarios


Aims: To evaluate the impact of addition of rapid tests for tuberculosis (TB) to mass screening and passive case finding on the burden of TB in high-incidence prisons of Azerbaijan. Materials and methods: All new and relapse TB cases notified in 01.01.2009-31.12.2015 were retrospectively included. Results: 2,315 TB patients were identified in 19 prisons. Implementation of the rapid tests to the case finding algorithms lead to 3-, 10- and 5-fold decrease in the annual rates of the notified, smear-positive and RIF-resistant TB cases, respectively. After introduction of rapid tests into the screening algorithms, there were significant linear trends towards decrease in the notified (p=0.009), smear-positive (p=0.011) and RIF-resistant TB cases (p=0.02) with the annual rates of decrease (95% confidence interval (CI)) being -435 (-614; -255), -356 (-517; -195), and -99 (-160; -38), respectively. Utilization of rapid tests also significantly increased treatment success with first-line drugs among all cases, cases detected by mass screening and those, detected by passive case finding [adjusted odds ratio (aOR)=2.38, 95% CI:1.86-3.05, aOR=4.56, 95% CI:2.64-7.89 and aOR=2.60, 95% CI:1.81-3.75, respectively]. Conclusions: Introduction of rapid tests into the screening lead to decline in the burden of TB and RIF-resistance, and improved outcomes of treatment with first-line drugs in prisons


Assuntos
Humanos , Tuberculose/diagnóstico , Mycobacterium tuberculosis/isolamento & purificação , Prisioneiros/estatística & dados numéricos , Testes Imediatos/estatística & dados numéricos , Azerbaijão/epidemiologia , Tuberculose/epidemiologia , Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia , Tuberculose Latente/epidemiologia , Testes Imunológicos/métodos
12.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 35(10): 655-658, dic. 2017. tab
Artigo em Espanhol | IBECS | ID: ibc-169566

RESUMO

La mayor parte de los casos de fiebre de duración intermedia (FDI) en España corresponden a enfermedades infecciosas (principalmente fiebre Q y rickettsiosis). En la práctica clínica el diagnóstico causal de estas entidades se basa en el inmunodiagnóstico, con una escasa utilidad en fases precoces. Por ello, el objetivo de este trabajo fue la evaluación de la utilidad de técnicas moleculares en el diagnóstico precoz de fiebre Q y rickettsiosis en pacientes con FDI. Se estudió mediante PCR la presencia de material genético de Coxiella burnetii y Rickettsia spp. en muestras sanguíneas de 271 pacientes con FDI. La especificidad de ambas técnicas es elevada, permitiendo el diagnóstico en casos no diagnosticados mediante detección de anticuerpos específicos. Estos datos sugieren que el empleo de técnicas moleculares, con una adecuada selección de la muestra de estudio y el empleo de cebadores adecuados, es un elemento útil en el diagnóstico precoz de las principales causas de FDI, principalmente si la serología es negativa o no es concluyente (AU)


Most cases of fever of intermediate duration (FDI) in Spain are associated with infectious diseases (mainly Q fever and rickettsia infections). In clinical practice, the causal diagnosis of these entities is based on immunodiagnostic techniques, which are of little help in the early stages. Therefore, the aim of this study was to evaluate the usefulness of molecular techniques for the early diagnosis of Q fever and rickettsia diseases in patients with FDI. A PCR method was used to detect the presence of genetic material of Coxiella burnetii and Rickettsia spp. in blood specimens from 271 patients with FDI. The specificity of both techniques is high, allowing diagnosis in cases undiagnosed by specific antibodies detection. These data suggest that the use of molecular techniques, with proper selection of the study specimen, and using appropriate primers is a useful tool in the early diagnosis of the main causes of FDI, especially if serology is negative or inconclusive (AU)


Assuntos
Humanos , Febre Q/diagnóstico , Febre Q/microbiologia , Diagnóstico Precoce , Infecções por Rickettsiaceae/microbiologia , Coxiella burnetii/isolamento & purificação , Infecções por Rickettsiaceae/complicações , Testes Imunológicos/métodos , Testes Sorológicos/métodos
17.
Allergol. immunopatol ; 44(4): 286-291, jul.-ago. 2016. graf, tab
Artigo em Inglês | IBECS | ID: ibc-154428

RESUMO

Background: Chronic urticaria can be the initial clinical presentation of a number of different diseases. The objective of the present study was to report the associated diseases during a ten-year clinical-laboratory follow-up in patients with an initial diagnosis of chronic spontaneous urticaria (CSU) of unknown cause. Methods: A prospective, longitudinal cohort study with a ten-year clinical-laboratory follow-up was conducted. Patients with a history of urticarial plaques of over six weeks presenting as the only clinical symptom were selected. Individuals with other clinical conditions, urticaria of known causes or chronic physical urticaria were excluded. The following tests were initially performed: haemogram, urine type I, stool parasite exam and sedimentation rate. The following exams were ordered during follow-up: PPD; urine culture; serology tests; antithyroid and antinuclear antibodies, rheumatoid factor, lupus anticoagulant; thyroid hormones; serum immunoglobulin; paranasal sinus and thorax radiographs; testing for BK and Helicobacter pylori; and prick tests. Results: Infections were diagnosed in 29% of patients (syphilis, parasitosis, H. pylori, urinary infection, tuberculosis, hepatitis B and C); autoimmune diseases in 21% (thyroiditis, rheumatoid arthritis and antiphospholipid antibody syndrome); primary immunodeficiencies in 4% (IgA and IgG2 deficiencies); and chronic myeloid leukaemia in 1%. At ten-years of follow-up, the urticaria diagnosis was CSU of unknown cause in 45% of the cases. Conclusion; This ten-year clinical-laboratory follow-up of 100 individuals with chronic urticaria as the initial diagnosis revealed the presence of associated diseases in over half of the cases. The most prevalent diseases were infections and autoimmune diseases besides primary immunodeficiencies and blood diseases (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Adulto , Urticária/complicações , Urticária/diagnóstico , Urticária/imunologia , Doenças Autoimunes/imunologia , Leucemia Mieloide de Fase Crônica/complicações , Leucemia Mieloide de Fase Crônica/imunologia , Testes Imunológicos/métodos , Biópsia/métodos , Imunoglobulina G/análise , Estudos Prospectivos , Seguimentos , Estudos Longitudinais , Estudos de Coortes
18.
Rev. Asoc. Esp. Espec. Med. Trab ; 25(1): 10-18, mar. 2016. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-151429

RESUMO

Objetivo: Cribado del cáncer colorrectal mediante la detección de sangre oculta en heces (SOH) con test inmunoquímico, en trabajadores mayores de 50 años. Material y métodos: Estudio descriptivo y transversal de 1.065 trabajadores mayores de 50 años, durante dos años. Se les hizo un test inmunológico de SOH, de muestra única; los que dieron positivo fueron sometidos a colonoscopia completa y estudio anatomopatológico en su caso. Los datos obtenidos se trataron estadísticamente con una regresión logística multivariante, confrontándose la variable dependiente (SOH) con otras independientes (edad, sexo, antecedentes médicos, parámetros bioquímicos, etc.) para comprobar la existencia o no de significación estadística. Resultados: 115 trabajadores (10,8% de la población estudiada) dieron positivo al test de SOH. En 36 casos se detectaron pólipos colorrectales, (28 hombres y 8 mujeres) y se diagnosticó un adenocarcinoma 'in situ' de colon en una mujer. Conclusión: El screening de SOH es coste-efectivo a partir de los 50 años de edad, por lo que se recomienda su empleo en los reconocimientos médico-laborales periódicos (AU)


Objective: Screening of colorectal cancer in a working population, using the fecal occult blood by immunochemical test. Material and methods: it is made a descriptive and transversal study of workers over 50 years old. The population sample consisted in 1,065 people. All of them made the test once. The positive ones were sent to the gastroenterologist in order to have a colonoscopy.The results were studied with a multivariate logistic regression, to determine the existence or not of statistical significance among the test and other variables took into consideration. Results: 115 workers got a positive test. In 36 cases it was found a colonic polyposis affection, and in one case was detected an 'in situ' adaenocarcinoma colonic. Conclusions: The test has high cost effectiveness and that is why it is recommended to apply it as a preventive diagnostic method in workers older 50 years (AU)


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Neoplasias Colorretais/imunologia , Neoplasias Colorretais/patologia , Neoplasias Colorretais/terapia , Programas de Rastreamento/análise , Programas de Rastreamento/métodos , Testes Imunológicos/instrumentação , Testes Imunológicos/métodos , Testes Imunológicos , Análise Custo-Benefício/métodos , Análise Custo-Benefício , Avaliação de Custo-Efetividade , Colonoscopia/instrumentação , Colonoscopia/métodos , Colonoscopia , Medicina do Trabalho/instrumentação , Medicina do Trabalho/métodos , Epidemiologia Descritiva , Estudos Transversais , Espanha
19.
Allergol. immunopatol ; 44(1): 46-53, ene.-feb. 2016. tab, graf
Artigo em Inglês | IBECS | ID: ibc-147483

RESUMO

Background: Early identification of septic patients at risk of mortality is important in their prognosis. Objective: Identification of septic patients at risk of mortality in Pediatric Intensive Care Units (PICUs) at Cairo University Hospitals, through measuring the levels of certain immunological parameters. Methods: A hospital-based prospective cohort study was conducted in two PICUs at Cairo University Hospitals; all patients with diagnosis of severe sepsis or septic shock on admission were included. A total of 57 patients were prospectively followed at the selected PICUs and their demographic and clinical data were recorded. Microbiological and immunological workup (at days 1 and 7) was conducted for all patients to detect the causative organism of sepsis and to measure the levels of immunoglobulins (IgG, IgM and IgA), complement factors (C3 and C4), mature lymphocyte subpopulations (CD3+) and natural killer (NK) cells (CD3-CD16+CD56+), respectively. Results: Mortality rate was 24.6%; the most frequent causes of death were multi-organ dysfunction and refractory shock. PELOD and PRISM III scores were significantly higher among non-survivors. At day 1, non-survivors had significantly higher levels of IgG, C4 and NK cells than survivors. However, from day 1 to day 7, survivors had a progressive increase in most of the immunological parameters (IgG, IgM, C4and CD3+ T lymphocytes). Survival curve analysis revealed the significant predictive ability of NK cells to detect early mortality. Conclusion: Monitoring the levels of cellular and humoral immunological parameters together with assessing PELOD and PRISM III scores can significantly affect prognosis and survival of septic children (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Criança , Sepse/complicações , Sepse/epidemiologia , Sepse/imunologia , Prognóstico , Imunoglobulina G , Imunoglobulina M/imunologia , Imunoglobulina A , Imunoglobulina A/imunologia , Imunidade Humoral/imunologia , Testes Imunológicos/métodos , Testes Imunológicos/normas , Indicadores de Morbimortalidade , Unidades de Terapia Intensiva Pediátrica
20.
J. investig. allergol. clin. immunol ; 26(1): 31-39, 2016. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-150187

RESUMO

Background: Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy. Objective: To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy. Methods: sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cor a 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges. Results: Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP-sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P<.05). Similar rates of Cor a 8 and Jug r 3 sensitization were detected by both techniques. Conclusions: The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved (AU)


Introducción: La utilidad clínica del diagnóstico por componentes no ha sido evaluada en el estudio de la alergia a frutos secos (FS). Objetivo: Evaluar la capacidad diagnóstica de una micromatriz comercial de proteínas alergénicas en la alergia a cacahuete, avellana y nuez. Métodos: Se determinó la sIgE en pacientes alérgicos a FS mediante la micromatriz ISAC 112, e ImmunoCAP en los pacientes con sIgE negativa frente a los componentes de ISAC. Además, se realizó ImmunoCAP frente a Ara h 9, Cor a 8 y Jug r 3 en un subgrupo de pacientes sensibilizados a LTP. La sIgE detectada por ImmunoCAP fue comparada con los rangos de ISAC. Resultados: La mayoría de los alérgicos a cacahuete (66,7%), avellana (80,5%) y nuez (84%) estaba sensibilizados a su LTP. Sin embargo, no se detectó sIgE frente a los componentes de ISAC en el 33,3% de alérgicos a cacahuete, 13,9% de alérgicos a avellana y 13,6% de los alérgicos a nuez. El ImmunoCAP permitió detectar sIgE a Ara h 9 en 61,5%, Cor a 8 en 60% y Jug r 3 en 83,3% de los ISAC negativo. En el subgrupo LTP, ImmunoCAP (94,4%) fue superior a ISAC (72,2%) en la detección de sIgE a Ara h 9 (p<0,05). La sIgE frente a Cor a 8 y Jug r 3 fue detectada de forma similar por ambas técnicas. Conclusiones: La micromatriz ISAC es adecuada para el diagnóstico de alergia a avellana y nuez. La sensibilidad del componente Ara h 9 de ISAC debe ser mejorada (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Hipersensibilidade a Noz/imunologia , Arachis/imunologia , Hipersensibilidade a Amendoim/imunologia , Corylus/imunologia , Hipersensibilidade/diagnóstico , Hipersensibilidade/imunologia , Testes Imunológicos/instrumentação , Testes Imunológicos/métodos , Testes Imunológicos , Técnicas Imunológicas/métodos , Testes Imunológicos/classificação , Testes Imunológicos/estatística & dados numéricos , Testes Imunológicos/normas , Técnicas Imunológicas/instrumentação , Técnicas Imunológicas/normas , Técnicas Imunológicas
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