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1.
Endodoncia (Madr.) ; 37(3): 23-30, dic. 2019. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-189959

RESUMO

OBJETIVO: El objetivo del presente estudio fue evaluar la conformación obtenida por los sistemas TruNatomy, WaveOne Gold y ProTaper Gold en conductos curvos simulados de Endo Training Blocks. MATERIALES Y MÉTODOS: Se utilizaron 30 Endo Training Blocks con conductos curvos simulados divididos en 3 grupos de 10 muestras cada uno, instrumentados con TruNatomy, WaveOne Gold y ProTaper Gold respectivamente. Todos los sistemas se emplearon siguiendo el protocolo sugerido por el fabricante. Los conductos simulados se irrigaron con 3 ml de agua destilada a cada cambio de instrumento y al finalizar la preparación. Se utilizó para este procedimiento la aguja plástica del sistema TruNatomy. Las muestras fueron teñidas con tinta china a fin de poder visualizar correctamente el espacio conformado. A continuación, se realiza-ron mediciones del ancho de los conductos curvos simulados a 7 mm, 11 mm y 14 mm del acceso coronal con un software de imágenes. Para la evaluación estadística se llevó a cabo el análisis de variancia de medidas repetidas y la prueba de Tukey. RESULTADOS: Se observaron diferencias estadísticamente significativas entre el grupo de TruNatomy y los otro dos. Las diferencias fueron significativas entre los tercios de cada grupo (P < 0.05). CONCLUSIÓN: El uso del sistema TruNatomy genera una conformación más conservadora que el WaveOne Gold y Protaper Gold en los tres tercios del conducto simulado


OBJECTIVE: The aim of this study was to evaluate the shaping ability performed by TruNatomy, WaveOne Gold and ProTaper Gold systems in simulated curved canals of Endo Training Blocks. MATERIALS AND METHODS: Thirty Endo Training Blocks with simulated curved canals were used. After dividing into 3 groups of 10 samples each, blocks were instrumented with TruNatomy, WaveOne Gold and ProTaper Gold respectively. All systems were used following the protocol suggested by the manufacturer. The canals were irrigated with 3 ml of distilled water between each instrument and as a final rinse. The TruNatomy plastic needle was used for this procedure. The samples were dyed with Chinese ink in order to visualize the final shape. The wide measurements of the simulated curved canals were carried out at 7 mm, 11 mm and 14 mm of the coronal access using image software. For the statistical evaluation the analysis of variance of repeated measures and the Tukey test were done. RESULTS: Statistically significant differences were observed be-tween the TruNatomy group and the other two. The differences were significant between thirds of each group (P < 0.05). CONCLUSIONS: The use of the TruNatomy system generates a more conservative shape than the WaveOne Gold and ProTaper Gold in the three thirds of the simulated canals


Assuntos
Humanos , Instrumentos Odontológicos , Ligas Dentárias , Desenho de Equipamento , Teste de Materiais , Valores de Referência , Reprodutibilidade dos Testes
2.
Med. oral patol. oral cir. bucal (Internet) ; 23(6): e646-e655, nov. 2018. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-176386

RESUMO

Background: The purpose of this study was to evaluate nanohardness and viscoelastic behavior of dentin surfaces treated with two canal sealer cements for dentin remineralization. Material and Methods: Dentin surfaces were subjected to: I) 37% phosphoric acid (PA) or II) 0.5 M ethylenediaminetetraacetic acid (EDTA) conditioning prior to the application of two experimental hydroxyapatite-based cements, containing sodium hydroxide (calcypatite) or zinc oxide (oxipatite), respectively. Samples were stored in simulated body fluid during 24 h or 21 d. The intertubular and peritubular dentin were evaluated using a nanoindenter to assess nanohardness (Hi). The load/displacement responses were used for the nano-dynamic mechanical analysis to estimate complex modulus (E*) and tan delta (δ). The modulus mapping was obtained by imposing a quasistatic force setpoint to which a sinusoidal force was superimposed. AFM imaging and FESEM analysis were performed. Results: After 21 d of storage, dentin surfaces treated with EDTA+calcypatite, PA+calcypatite and EDTA+oxipatite showed viscoelastic discrepancies between peritubular and intertubular dentin, meaning a risk for cracking and breakdown of the surface. At both 24 h and 21 d, tan δ values at intertubular dentin treated with the four treatments performed similar. At 21 d time point, intertubular dentin treated with PA+oxipatite achieved the highest complex modulus and nanohardness, i.e., highest resistance to deformation and functional mineralization, among groups. Conclusions: Intertubular and peritubular dentin treated with PA+oxipatite showed similar values of tan δ after 21 d of storage. This produced a favorable dissipation of energy with minimal energy concentration, preserving the structural integrity at the dentin surface


No disponible


Assuntos
Humanos , Cemento Dentário/fisiologia , Calcificação Fisiológica , Dentina/metabolismo , Cimentos para Ossos/química , Cimento de Fosfato de Zinco/administração & dosagem , Fenômenos Biomecânicos , Teste de Materiais
3.
Arch. esp. urol. (Ed. impr.) ; 69(8): 553-564, oct. 2016. ilus
Artigo em Espanhol | IBECS | ID: ibc-156800

RESUMO

Una de los principales anhelos en el campo de los catéteres y stents urinarios es dotarlos de características biodegradables. Ya que se entiende como un fracaso de estos dispositivos la necesidad de retirada, el síndrome de catéter olvidado, así como los efectos adversos que provocan los dispositivos permanentes tras cumplir su finalidad. Los esfuerzos centrados en nuevos diseños, recubrimientos, así como biomateriales buscan incrementar la biocompatibilidad de estos dispositivos internos. En los últimos tiempos los principales retos con respecto a los dispositivos biodegradables ureterales han avanzado en la correcta dirección. Así, la tasa de degradación se ha conseguido modular gracias a los nuevos biomateriales y al empleo de copolímeros que permiten elegir el tiempo de permanencia al igual que se programa con los catéteres doble jota convencionales. La biocompatibilidad ha mejorado de la mano de nuevos polímeros que se adaptan mejor al medio urinario. Por último uno de los principales problemas, la eliminación de fragmentos degradados, demuestra a nivel experimental que con nuevos diseños que provocan la degradación controlada, de distal a proximal, empleando el trenzado y combinando copolímeros, la degradación se puede provocar por dilución, reduciendo la fragmentación a los últimos estadios de vida de la prótesis. Además se demuestra que potencialmente los catéteres biodegradables pueden provocar menor infección urinaria, menor incrustación y previsiblemente disminuirán la morbilidad de los catéteres, ya que su proceso de degradación reduce los efectos adversos. Con respecto al desarrollo de stents uretrales biodegradables se necesita encontrar biomateriales que permitan mantener sus propiedades biomecánicas a largo plazo, manteniendo la luz uretral abierta tanto en pacientes con HBP como en los casos de estenosis uretral. Se ha conseguido modular el tiempo de degradación de las prótesis, pero todavía la aparición de hiperplasia urotelial polipoidea es una constante en las fases iniciales post-implantación. El desarrollo de stents liberadores de fármaco, antiinflamatorio o antiproliferativos, al igual que stents biodegradables biorrecubiertos es un campo del que se espera venga la solución a estos efectos adversos. Son por tanto muchos aspectos que deben ser mejorados para obtener los ansiados stents biodegradables, pero en los últimos años se han ido cumpliendo hitos, gracias a los avances en Bioingeniería, que permiten vislumbrar en un futuro próximo soluciones seguras y eficaces (AU)


One of the main wishes in the field of urinary catheters and stents is to arm them with biodegradable characteristics because we consider a failure of these devices the need for retrieval, the forgotten catheter syndrome as well as the adverse effects permanent devices cause after fulfilling their aim. The efforts focused in new designs, coatings and biomaterials aim to increase the biocompatibility of theses internal devices. Lately, there have been correct advances to answer the main challenges regarding biodegradable ureteral devices. Thus, modulation of the rate of degradation has been achieved thanks to new biomaterials and the use of copolymers that enable to choose the time of permanence as it is programmed with conventional double J catheters. Biocompatibility has improved with the use of new polymers that adapt better to the urine. Finally, one of the main problems is elimination of degraded fragments and experimentally it has be demonstrated that new designs elicit controlled degradation, from distal to proximal; using stranding and combination of copolymers degradation may be caused by dilution, reducing fragmentation to the last stages of life of the prosthesis. Moreover, it has been demonstrated that biodegradable catheters potentially may cause less urinary tract infection, less encrustation and predictably they will diminish catheter morbidity, since their degradation process reduces adverse effects. Regarding the development of biodegradable urethral stents, it is necessary to find biomaterials that enable maintaining their biomechanical properties in the long term, keeping open the urethral lumen both in patients with BPH and urethral stenosis. Modulation of the time of degradation of the prosthesis has been achieved, but the appearance of urothelial hyperplasia is still a constant in the initial phases after implantation. The development of drug eluting stents, anti-proliferative or anti-inflammatory, as well as biodegradable stents biocoated is a field from which it is expected the arrival of the solution of theses adverse effects. Therefore, many features need to be improved to obtain biodegradable stents, but over the last years some turning points have been accomplished thanks to the advances in Bioengineering, allowing to foresee safe and effective solutions in the nearest future (AU)


Assuntos
Humanos , Masculino , Feminino , Implantes Absorvíveis/classificação , Implantes Absorvíveis/normas , Implantes Absorvíveis , Cateteres/normas , Cateteres , Cateteres Urinários , Fluoroscopia/instrumentação , Fluoroscopia/métodos , Ureteroscopia/instrumentação , Ureteroscopia/métodos , Stents , Teste de Materiais/métodos , Teste de Materiais/normas , Polímeros/uso terapêutico , Hiperplasia
4.
Ars pharm ; 57(3): 137-142, jul.-sept. 2016. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-158251

RESUMO

Aim: Current experimental investigation is dedicated to prepare microspheres with small size and good sphericity by Phase Separation method using Isoniazid (INH) as model drug. Silk fibroin has unique intrinsic qualities like biodegradability, biocompatibility or release properties and their tunable drug loading capacity. The delivery loading proficiency of the drug molecules in silk spheres be contingent on their charge, and hydrophobicity or subsequent in altered drug release profiles. Methods: In the present work Isoniazid loaded silk fibroin microsphere was prepared by using phase separation method. Microsphere was evaluated for Ultraviolet-visible spectroscopy, Fourier Transform infrared spectroscopy, Entrapment efficiency, Scanning electron microscopy Studies. Results: Scanning electron microscopy studies revealed that Isoniazid Loaded Silk Fibroin Microspheres were spherical. Entrapment Efficiency of Isoniazid loaded Microspheres of different Formulation from F1 to F5 was in range of 53 to 68 %. F3 showed 68.47 % entrapment Efficiency and the optimized formulation drug release was 93.56 % at 24 hours. Conclusion: Experimental report disclosed a new aqueous based formulation method for silk spheres with controllable shape or size and sphere. Isoniazid loaded silk microspheres may act as ideal nano formulation with elaborated studies


Objetivo: La investigación experimental en curso está dedicada a la preparación de microesferas de pequeño tamaño y buena esfericidad mediante el método de separación de fases con isoniazida (INH) como fármaco modélo. La fibroina de seda tiene cualidades intrínsecas únicas como la biodegradabilidad, biocompatibilidad o propiedades de liberación y su capacidad de carga de fármacos ajustable. La aptitud de entrega de carga de las moléculas de fármaco en las esferas de seda estar supeditada a su carga, y la hidrofobicidad o subsiguiente alteración en los perfiles de liberación de fármacos. Métodos: En el presente trabajo la microesfera de fibroina de seda cargada de isoniazida fue preparada utilizando el método de separación de fases. La microesfera fue evaluada por espectroscopia ultravioleta-visible, espectroscopia infrarroja con transformado de Fourier, se midió la eficiencia de atrapamiento y se estudios mediante microscopia electrónica de barrido. Resultados: Estudios con el microscopio de escaneo de electrones revelaron que las microesferas de fibroina cargada de isoniazida eran esféricas. La eficacia de atrapamiento de las microesferas de formulación diferente de F1 a F5 estuvo en el rango de 53 a 68 %. F3 mostró un 68,47 % de eficiencia de atrapamiento y tras optimizar la formulación de liberación de fármacos fue de 93,56 %, a las 24 horas


Assuntos
Isoniazida/síntese química , Isoniazida/farmacologia , Fibroínas/farmacologia , Desenvolvimento Experimental , Teste de Materiais/métodos , Projetos de Pesquisa , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Microscopia Eletrônica de Varredura/instrumentação , Química Farmacêutica/métodos
5.
Med. oral patol. oral cir. bucal (Internet) ; 20(1): 88-93, ene. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-132062

RESUMO

Objectives: To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application. Study Design: Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: GroupI: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscope (SEM) and confocal laser scanning electron microscope( CLSM).Osteoblast adhesion, proliferation and cell morphology were determined by means of fluorescence microscopy with Image-Pro Plus software and SEM. Results: Group II presented the roughest discs, while the least rough were Group III. Cell adhesion was greatest in Group II. The addition of melatonin improved cell proliferation. Conclusions: 1. Surface treatments (dual acid etching, calcium phosphate impaction) increase surface roughness in comparison with machined titanium.2. Greater surface roughness tends to favor cell adhesion after 24-hour cell culture.3. The addition of melatonin tends to favor osteoblast proliferation (AU)


Assuntos
Humanos , Titânio/farmacocinética , Osteoblastos , Melatonina/farmacocinética , Osseointegração/fisiologia , Implantação Dentária/métodos , Teste de Materiais/métodos
6.
Trauma (Majadahonda) ; 25(4): 232-236, oct.-dic. 2014. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-132825

RESUMO

Objetivo: Evaluar, mediante ensayos in vivo, la biocompatibilidad y el grado de penetración ósea en la microestructura porosa del carburo de silicio (SiC). Material y métodos: se implantaron en cóndilos femorales de conejos cilindros de SiC obtenidos a partir de maderas de pino y sapeli. Como material de referencia fueron utilizados cilindros de titanio. El sacrificio de los animales se realizó a las 12 semanas de implantación y se procedió al examen histológico de las muestras. Resultados: Comprobamos un crecimiento de trabéculas óseas, tanto en la superficie del implante como a través de su estructura porosa, sin apreciarse signos de inflamación ni aparición de tejido fibroso alrededor de la muestra. Estos resultados son confirmados mediante microscopía electrónica de barrido (SEM) y análisis de rayos X por dispersión de energías. Conclusión: El carburo de silicio biomórfico es una cerámica con excelentes propiedades mecánicas y porosidad interconectada que le confiere un especial atractivo de cara a sus aplicaciones biomédicas en implantes ortopédicos (AU)


Objective: To evaluate, using in vivo tests, the biocompatibility and the degree of bone penetration into the porous microstructure of silicon carbide (SiC). Material and methods: For this purpose cylindres of SiC obatained from wood of pine and sapeli were implanted in femoral condyles of rabbits. As reference material we used titanium cylinders. The slaughter of animals was performed at 12 weeks of implantation and proceeded to the histological examination of the samples. Results: We see a growth of bone trabeculae, both on the surface of the implant and through its porous structure, without signs of inflammation or appearance of fibrous tissue around the samples. These results are confirmed by scanning electron microscopy scanning (SEM) and X-ray analysis by dispersion of energies (EDS). Conclusion: Biomorphic silicon carbide (SiC) is a ceramic with excellent mechanical properties and interconnected porosity which gives a special attraction for their biomedical applications in orthopaedic implants (AU)


Assuntos
Animais , Masculino , Feminino , Coelhos , Materiais Biocompatíveis/análise , Materiais Biocompatíveis/uso terapêutico , Compostos de Silício/análise , Compostos de Silício/isolamento & purificação , Osseointegração , Osseointegração/imunologia , Teste de Materiais/métodos , Teste de Materiais/veterinária , Modelos Animais , Fêmur/cirurgia , Microscopia Eletrônica de Varredura/métodos , Microscopia Eletrônica de Varredura , Próteses e Implantes , Próteses e Implantes/veterinária
7.
Av. periodoncia implantol. oral ; 26(2): 83-89, ago. 2014. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-126671

RESUMO

El agrandamiento gingival es común entre los pacientes y puede ser causado por una variedad de factores etiológicos. La razón más común es la inflamación gingival provocada por mala higiene oral y alta carga bacteriana. Otros factores implicados son los medicamentos sistémicos, en donde se encuentran bloqueadores de los canales de calcio, inmunosupresores y anticonvulsivantes. Algunos crecimientos gingivales podrían estar asociados a condiciones hormonales como la pubertad, el embarazo o la diabetes o ser un síntoma de una enfermedad sistémica y en raras ocasiones el agrandamiento gingival se asocia a factores genéticos y hereditario llamado fibromatosis gingival. Otra causa del agrandamiento gingival se viene presentando en la consulta odontológica y es la relacionada con la aparatología ortodóncica fija, cuya causa específica no está totalmente definida aún. El presente artículo es una revisión narrativa cuyo objetivo es describir las generalidades del agrandamiento gingival y reportes en la literatura sobre la acción de la aparatología ortodóncica fija en el agrandamiento gingival, pues se presume del níquel como otro factor causal de esta patología (AU)


Gingival overgrowth is common among patients and can be caused by a variety of etiological factors. The most common reason is gingival inflammation caused by poor oral hygiene and high bacterial load. Other factors include systemic drugs, like channel blockers are calcium, immunosuppressants and anticonvulsants. Some gingival overgrowth are associated with hormonal conditions such as puberty, pregnancy or diabetes or be a symptom of a systemic disease and rarely the gingival overgrowth is associated with genetic factors, who is called hereditary gingival fibromatosis. Another cause of gingival overgrowth has been presented in dentistry and is related to fixed orthodontic appliances, whose specific cause is not fully defined yet. This article is a narrative review which aims to describe the generalities of gingival overgrowth and reports in the literature of the effect of fixed orthodontic appliances in gingival overgrowth because nickel probably is another factor causing this condition (AU)


Assuntos
Humanos , Crescimento Excessivo da Gengiva/etiologia , Níquel/efeitos adversos , Aparelhos Ortodônticos/efeitos adversos , Hiperplasia Gengival/etiologia , Doenças da Gengiva/etiologia , Corrosão , Materiais Biocompatíveis/efeitos adversos , Teste de Materiais
8.
Med. oral patol. oral cir. bucal (Internet) ; 18(4): 745-751, jul. 2013. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-114501

RESUMO

Objectives: The purpose of this study was to evaluate and compare the cytotoxic effects of ProRoot MTA and DiaRoot BA, a bioceramic nanoparticulate cement, on subcutaneous rat tissue. Study Design: Fifty Sprouge Dawley rats were used in this study. Polyethylene tubes filled with ProRoot MTA and DiaRoot BioAggregate, along with a control group of empty, were implanted into dorsal connective tissue of rats for 7, 15, 30, 60, and 90 days. After estimated time intervals the rats were sacrificed. The specimens were fixed, stained with hematoxylin and eosin, and then evaluated under a light microscope for inflammatory reactions and mineralization. Results: All groups evoked a severe to moderate chronic inflammatory reaction at 7 and 15 days, which decreased with time. Both the MTA and BioAggregate groups showed similar inflammatory reactions, except at 90 days when MTA showed statistically significant greater inflammation (p>0.05). The MTA group showed foreign body reaction at all times. Compared to BioAggregate, MTA showed significantly more foreign body reaction at 60 and 90 days (p<0.0001). After 30 days foreign body reaction of BioAggregate decreased significantly. Both MTA and BioAggregate groups showed similar necrosis at 7 and 15 days (p=0.094 and p=0.186 respectively). No necrosis was observed after 15 days. Similarly there was no fibrosis after 30 days for both MTA and BioAggregate groups (p>0.05). Conclusions: Since DiaRoot BioAggregate showed significantly better results than MTA, we can conclude that it is more biocompatible. However, further studies are required to confirm this result (AU)


Assuntos
Animais , Ratos , Citotoxinas/análise , Cimentos Dentários/análise , Materiais Biocompatíveis/análise , Tecido Conjuntivo , Tela Subcutânea , Teste de Materiais
9.
Enferm. nefrol ; 15(4): 271-276, oct.-dic. 2012. tab
Artigo em Espanhol | IBECS | ID: ibc-109002

RESUMO

Objetivo: Comparar la biocompatibilidad entre la hemodiafiltración en línea (HDF) y la hemodiafiltración con reinfusión endógena (HFR). Material y método: Estudio comparativo observacional en una población de 15 pacientes en hemodiálisis crónica elegidos al azar entre los pacientes de nuestra unidad. Se compararon cambios en el perfil hematológico, nivel de PCR y constantes vitales, pre y post hemodiálisis, tras someterse a ambas técnicas de hemodiafiltración. Se comparó las diferencias entre los parámetros estudiados pre y post hemodiálisis en cada técnica. Resultados: Los niveles de plaquetas descendieron más en la HDF (HDF -1,33 vs HFR -19,73 x103/mm3, p=0,005). El nivel de leucocitos disminuyó en la HDF y aumentó en la HFR (HDF -0,46 vs HFR +0,8 x103/ mm3; p=0,006). Respecto a la fórmula leucocitaria hubo resultados dispares: segmentados HDF -1,7 vs HFR +5,4%, p<0,001; linfocitos HDF +1,96 vs HFR -3,62%, p<0,001. Con la HFR disminuyeron menos los niveles de PCR (HDF -0,05 vs HFR -0,001 mg/ dl; p=NS). En lo referente a las constantes vitales, la tensión arterial sistólica descendió más en la HFR que en la HDF (HDF -9,93 vs HFR -10,33 mmHg; p<0,001), a la inversa que la diastólica (HDF -5,2 vs HFR -3 mmHg; p=0,007) y la frecuencia cardiaca (HDF -1,46 vs HFR +1,73 lpm; p=NS). La temperatura corporal aumentó más con la HDF que con la HFR (HDF +0,35 vs HFR +0,06 ºC; p=NS). Conclusiones: Según nuestros resultados la HFR parece más biocompatible que la HDF, probablemente derivado por la reinfusión exógena de la HDF (AU)


Aim: To compare biocompatibility between online hemodiafiltration (HDF) and hemodiafiltration with endogenous reinfusion. Methods: Observational comparative study in a population of 15 chronic hemodialysis patients randomly selected among the patients in our unit. We compared changes in hematological profile, CRP level and vital signs, pre and post hemodialysis, after undergoing both hemodiafiltration techniques. Comparing the differences between the parameters studied before and after each hemodialysis technique. Results: Platelet levels decreased more in the HDF (HDF -1,33 vs HFR -19,73 x103/mm3, p=0,005). Leukocyte levels decreased in the HDF and increased with HFR (HDF -0,46 vs HFR +0,8 x103/mm3; p=0,006). Regarding the leukocyte formula had mixed results: segmented HDF -1,7 vs HFR +5,4%, p<0,001; lymphocytes HDF +1,96 vs HFR -3,62%, p<0,001. With the HFR decreased CRP levels less (HDF -0,05 vs HFR -0,001 mg/dl; p= NS). Regarding vital signs, systolic blood pressure decreased more in the HFR than HDF (HDF -9,93 vs HFR -10,33 mmHg; p<0,001), conversely that the diastolic blood pressure (HDF -5,2 vs HFR -3 mmHg; p=0,007), and heart rate (HDF -1,46 vs HFR +1,73 lpm; p=NS). Body temperature increased more with the HDF that the HFR (HDF +0,35 vs HFR +0,06 ºC; p=NS). Conclusions: According to our results the HFR seems more biocompatible than the HDF, probably derived by exogenous reinfusion of HDF (AU)


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Teste de Materiais/métodos , Teste de Materiais/normas , Hemodiafiltração/instrumentação , Hemodiafiltração/métodos , Hemodiafiltração , Reação em Cadeia da Polimerase/métodos , Contagem de Leucócitos/métodos , Contagem de Leucócitos/tendências , Leucopenia/complicações , Leucopenia/diagnóstico , Hemodiafiltração/normas , Hemodiafiltração/tendências , Reação em Cadeia da Polimerase/normas , Temperatura Corporal/fisiologia , Reação em Cadeia da Polimerase/tendências , Temperatura Corporal , Frequência Cardíaca/fisiologia , Leucopenia/fisiopatologia
10.
An. R. Acad. Farm ; 78(1): 82-97, ene.-mar. 2012. ilus
Artigo em Espanhol | IBECS | ID: ibc-102743

RESUMO

La pectina es un polisacárido de origen vegetal que posee excelentes características de gelificación, y biocompatibilidad, por lo que ha sido ampliamente estudiada como excipiente en diversas formas farmacéuticas para modular la liberación de moléculas activas. La pectina permanece intacta en la parte alta del tracto gastrointestinal, y sólo es degradada por la microflora del colon. Por tal motivo, se ha incluido en esta revisión el desarrollo de diferentes formulaciones de liberación colónica de fármacos basadas en este polisacárido para tratar afecciones locales como el cáncer de colon y la enfermedad de Crohn. También se incluyen diversos estudios acerca de aplicaciones terapéuticas de la pectina como coadyuvante en diferentes patologías digestivas como el reflujo gastroesofágico y la diarrea persistente. El efecto que, sobre los niveles de glucosa, insulina y lípidos en plasma, puede tener una dieta rica en pectina es así mismo analizado(AU)


Pectin: Pharmaceutical and Therapeutic UsesPectin is a polysaccharide of vegetarian origin, combining both excellent gelation and biocompatibility characteristics, and thus has been widely studied as an excipient in different pharmaceutical forms in order to modulate the release of active molecules. Pectin remains intact in the upper gastrointestinal tract and only gets degraded by colonic microflora. Hence, different formulations based on this polysaccharide, which have been developed to target drugs to the colon and treat local affections like colon cancer and Crohn’s disease, were included in this review. Also, different studies on therapeutic uses of pectin as an adjuvant in various digestive pathologies such as the gastro-esophageal reflux and persistent diarrhea have been discussed. The effects of a rich pectin-diet over the glucose, insulin and lipid plasma levels are also commented(AU)


Assuntos
Animais , Masculino , Feminino , Camundongos , Pectinas/uso terapêutico , Polissacarídeo-Liase/uso terapêutico , Hidrogéis/uso terapêutico , Anticarcinógenos/uso terapêutico , Pectinas/metabolismo , Pectinas/farmacologia , Pectinas/farmacocinética , Colesterol/uso terapêutico , Teste de Materiais/métodos
12.
Artigo em Inglês | IBECS | ID: ibc-98915

RESUMO

Objectives: One of the most important factors for suitable materials for pulp therapy is biocompatibility. Two histopathologic methods of Cox and Federation Dentaire International (FDI) were used to evaluate inflammation. In Cox method, density of inflammatory cells, tissue reactions like fibrosis, vascular responses like congestion and fibrin extravasation have been used to evaluate inflammatory reactions. The aim of this study was to compare the accuracy of pathologists’ interpretations using two different methods. Study design: Three pathologists observed the degree of inflammation in 225 histopathologic sections. These sections showed inflammation in subcutaneous connective tissue of rats adjacent to polyethylene tubes, filled with white or gray mineral trioxide aggregate. Empty tubes served as controls. Samples were harvested after 7-, 15-,30-, 60-, and 90-days. All pathologists examined the sections under a light microscope (Carl Zeiss, Oberkochen,Germany) at ×400 magnifications. Chi-Square test was used to evaluate the difference between inflammation grades when one pathologist used two methods. Cohen’s Kappa value was used to measure agreement of three pathologists to recognize the degrees of inflammations when using one of the methods(..) (AU)


Assuntos
Humanos , Imuno-Histoquímica/métodos , Tela Subcutânea/imunologia , Teste de Materiais/métodos , Inflamação/induzido quimicamente , Materiais Revestidos Biocompatíveis/análise , Tecido Conjuntivo/imunologia
13.
Med. oral patol. oral cir. bucal (Internet) ; 17(1): 129-134, ene. 2012. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-98929

RESUMO

This study examined whether the number and type of sutures used in oral surgery influence two ad hoc variables(incision plane and displaced area), which are two variables related to whether the suture needle is suitable for the task. Seventy-five TB-15 needles were studied, which were used to suture between zero and three mucosa and/subperiosteal sutures, producing 15 groups with 5 needles in each one. The incision plane and displaced area were measured for each group, which are two variables related to how the needle has worn and altered. Statistical treatment was conducted using the Kruskal-Wallis H test to compare multiple values and the Mann-Whitney Utest to compare pairs. A multi-stage regression model was applied with the aim of predicting the changes in the dependent variables based on the number and type of sutures performed. The incision plane ranged from 126.67 to346.24ìm among the different groups. The displaced area was measured as being between 14 524.83ìm² and 128311.91ìm². The best predictive model for the incision plane obtained a coefficient of determination (R2) of 0.149,while it reached 0.249 for the displaced area. Subperiosteal sutures held more weighting among the variables studied. Mucosal sutures did not seem to greatly affect needle wear. Observations reported in this paper indicate that the needle should be changed after having performed two subperiosteal sutures, given the wear and change to the incision plane that is produced, which causes the needle’s cutting ability to reduce (AU)


Assuntos
Humanos , Técnicas de Sutura/instrumentação , Procedimentos Cirúrgicos Bucais/métodos , Agulhas , Teste de Materiais/métodos , Microscopia Confocal
14.
Clin. transl. oncol. (Print) ; 14(1): 50-59, ene. 2012. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-126101

RESUMO

INTRODUCTION: Chemotherapeutic drug-eluting beads (DEBs) are microspheres that are in clinical use for intraarterial chemoembolisation of liver cancer. Here we report on the biocompatibility and anti-tumour efficacy of DEBs after intratumoral application in a rat BT4Ca glioma model. METHODS AND RESULTS: Doxorubicin and irinotecan-eluting DEBs were suspended in a Ca(2+)-free aqueous alginate solution that provides a sol-gel transition when injected into the Ca(2+) rich brain tissue. In this way the DEBs are immobilised at the implantation site. Forced elution studies in vitro using a USP-4 flow-through apparatus demonstrated that the alginate excipient helped to reduce the burst effect and rate the elution from the beads. From the in vivo evaluation, doxorubicin DEBs demonstrated a significant local toxicity, while irinotecan-loaded DEBs showed good local tissue compatibility. Doxorubicin at higher concentrations and irinotecan-loaded DEBs were found to decrease tumour volume, increase survival time and decrease the Ki67 proliferation index of the tumour. Doxorubicin was shown by fluorescent microscopy to diffuse into the peritumoral tissue, but also penetrates along white matter tracts, to more distant areas. DISCUSSION: We conclude that the alginate suspension of irinotecan DEBs can be considered safe and effective in a clinical setting (AU)


Assuntos
Animais , Masculino , Feminino , Ratos , Alginatos/química , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias Encefálicas/tratamento farmacológico , Carga Tumoral , Glioma/tratamento farmacológico , Microesferas , Sistemas de Liberação de Medicamentos , Teste de Materiais , Neoplasias Encefálicas/mortalidade , Neoplasias Encefálicas/patologia , Camptotecina/análogos & derivados , Camptotecina/administração & dosagem , Doxorrubicina/administração & dosagem , Glioma/mortalidade , Glioma/patologia , Ácido Glucurônico/química , Ácidos Hexurônicos , Taxa de Sobrevida
15.
Rev. Soc. Esp. Enferm. Nefrol ; 14(4): 222-229, oct.-dic. 2011. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-93840

RESUMO

Introducción: En los últimos años se han ido sustituyendo las líneas de hemodiálisis esterilizadas con óxido de etileno por las esterilizadas con rayos gamma, dado que numerosos autores han demostrado que es un método más biocompatible. Al utilizar líneas esterilizadas con rayos gamma observamos que se formaban más coágulos que con las esterilizadas con óxido de etileno. Objetivos: Observar si hay diferencia en las dosis de heparina utilizadas y en la formación de coágulos en los circuitos extracorpóreos esterilizados con rayos gamma y óxido de etileno. Material y método: Hemos realizado un estudio cuasi-experimental, longitudinal y prospectivo, utilizando en el mismo paciente líneas de hemodiálisis esterilizadas con rayos gamma y con óxido de etileno. Se realizaron en cada paciente 12 sesiones de hemodiálisis consecutivas con cada tipo de línea. Después de cada sesión se valoraron las pérdidas hemáticas en las cámaras arteriales y venosas, así como las necesidades de heparina. Resultado: Los resultados obtenidos demuestran que la formación de coágulos es mayor en las líneas esterilizadas con rayos gamma y que sí se modificó la dosis de heparina aunque no fue estadísticamente significativa. Discusión: Mantener una buena anticoagulación del circuito extracorpóreo durante la diálisis permite: realizar un tratamiento clínicamente satisfactorio durante el tiempo requerido, evitar pérdidas estimadas altas y minimizar la heparina circulante disminuyendo los efectos secundarios (AU)


Introduction: In recent years, haemodialysis lines sterilized using ethylene oxide have been replaced with lines sterilized using gamma irradiation, as several authors have proven that it is a more biocompatible method. On using lines sterilized by gamma irradiation, we observed that more clots were formed than with lines sterilized with ethylene oxide. Aims: To observe whether there is a difference in the heparin doses used and in the formation of clots in the extracorporeal circuits sterilized using gamma irradiation and ethylene oxide. Material and method: We carried out a quasiexperimental, longitudinal and prospective study using haemodialysis lines sterilized by gamma irradiation and by ethylene oxide on the same patient. On each patient, 12 consecutive haemodialysis sessions with each type of line were carried out. After each session, the blood loss in the arterial and venous chambers was evaluated, together with the heparin needs. Results: The results obtained show that clot formation is higher in the lines sterilized using gamma irradiation and that the heparin dose was indeed modified, although it was not statistically significant. Discussion: Maintaining good anticoagulation of the extracorporeal circuit during dialysis makes it possible to: carry out a clinically satisfactory treatment during the time required, prevent estimated high losses and minimize the circulating heparin, reducing the side effects (AU)


Assuntos
Humanos , Masculino , Feminino , Anticoagulantes/uso terapêutico , Esterilização/instrumentação , Óxido de Etileno/uso terapêutico , Heparina/uso terapêutico , Soluções para Diálise/uso terapêutico , Diálise Renal/enfermagem , Raios gama/uso terapêutico , Teste de Materiais/métodos , Trombose/epidemiologia , Trombose/enfermagem , Eritropoetina/uso terapêutico , Estudos Prospectivos , Trombose/prevenção & controle , Esterilização/métodos , Óxido de Etileno/administração & dosagem , Estudos Longitudinais
17.
Med. oral patol. oral cir. bucal (Internet) ; 15(5): 788-790, sept. 2010. tab
Artigo em Inglês | IBECS | ID: ibc-95901

RESUMO

Objectives: Today many clinicians use both stainless-steel hand K-files and nickel- titanium rotary files during endodontic treatment. It is of great importance for the clinician to have confidence in the accuracy of an apex locator even if these files were used interchangeably. The purpose of this in-vitro study was to evaluate the accuracy of the Raypex5 electronic apex locator using stainless-steel hand K-file versus nickel-titanium rotary Mtwo file.Study design: Twenty straight and single canals of maxillary central teeth were used. Access cavities were prepared;actual working length were determined and compared with electronic working length obtained by means of apex locator Raypex5 using stainless-steel hand K-file and nickel-titanium rotary Mtwo file. Data was analyzed by paired T-test.Results: There was no significant difference between 15/0.02 stainless-steel hand K- file and 10/0.04 NiTi rotary Mtwo file for the mean differences between actual and electronic working length (p=0.126).Conclusions: Under the conditions of this in-vitro study, Raypex5 registered more measurements in acceptable range using 15/0.02 stainless-steel hand K- file and 10/0.04 NiTi rotary Mtwo file. It is possible to use them interchangeably without compromising the working length (AU)


No disponible


Assuntos
Humanos , Titânio/análise , Aço Inoxidável/análise , Instrumentos Odontológicos , Preparo de Canal Radicular/instrumentação , Teste de Materiais/métodos , Materiais Biocompatíveis/análise
18.
Ars pharm ; 51(supl.3): 203-207, jul. 2010.
Artigo em Espanhol | IBECS | ID: ibc-99478

RESUMO

Los magnetosomas (magnetoliposomas) han permitido en los últimos años un gran avance en la terapia del cáncer, gracias a su capacidad para transportar de forma eficiente diferentes agentes quimioterápicos hasta la masa tumoral. Estos sistemas no sólo se usan en el transporte de fármacos sino que también presentan importantes posibilidades en el diagnóstico de esta severa enfermedad, en terapia génica (transfección magnética o magnetofección) y en hipertermia. Sin embargo, su destino biológico, una vez que son administrados, se encuentra fuertemente condicionado por su geometría y propiedades fisicoquímicas. El presente trabajo se centra en la investigación de los principales aspectos relacionados con el diseño y utilización clínica de los magnetosomas. En concreto, estrategias de formulación, biocompatibilidad, toxicidad y situación actual a nivel preclínico(AU)


During the last years, magnetosomes (magnetoliposomes) have contributed to a significant improvement in cancer therapy, thanks to their capability to transport very efficiently the antitumor drug dose to the malignant cells. Interestingly, these systems also offer very promising possibilities in cancer diagnosis, gene therapy (magnetic transfection or magnetofection), and hyperthermia. However, the biological fate of magnetosomes, upon administration, is markedly conditioned by their geometry and physicochemical properties. This work is focussed on the investigation of the main aspects related to the engineering and clinical use of liposomes. Concretely, formulation strategies, biocompatibility, toxicity, and current state of the art at the preclinical level(AU)


Assuntos
Humanos , Masculino , Feminino , Lipossomos/metabolismo , Lipossomos/farmacologia , Lipossomos/farmacocinética , Neoplasias/tratamento farmacológico , Carga Tumoral , Transfecção/métodos , Transfecção , Febre/tratamento farmacológico , Teste de Materiais/métodos
19.
Med. oral patol. oral cir. bucal (Internet) ; 15(2): 407-412, mar. 2010. ilus
Artigo em Inglês | IBECS | ID: ibc-80252

RESUMO

Objective: The aim of this study was to carry out an in vivo assessment of bone ingrowth in two different typesof porous titanium -the first being completely porous, and the second with a porous surface and dense nucleus,manufactured by powder metallurgy- and to evaluate their mechanical properties. Study design: Ten scaffoldsfrom each group were submitted to metallographic analysis and compression tests. Next, two scaffolds of eachtype were inserted into 14 rabbits, which were sacrificed 8 weeks after surgery. The samples were submitted forhistological examination. Results: Metallographic analysis revealed interconnected pores, and the average interconnectedpore diameter was about 360 mm, with 36% total porosity. The totally porous titanium samples and thetitanium samples with porous surface and dense nucleus showed an average compressive strength of 16.19 MPaand 69.27 MPa, respectively. After 8 weeks, the animals showed bone ingrowth, even into the most internal pores.Conclusions: The pore morphology was effective in permitting bone ingrowth in both groups. Titanium scaffoldswith a porous surface and dense nucleus showed the best mechanical properties and most adequate interface (AU)


Assuntos
Animais , Coelhos , Osseointegração , Titânio , Fenômenos Biomecânicos , Teste de Materiais
20.
Endodoncia (Madr.) ; 28(1): 7-11, ene.-mar. 2010.
Artigo em Espanhol | IBECS | ID: ibc-102088

RESUMO

Objetivo: El propósito de este estudio fue determinar si la adición de cloruro de calcio (CaCl2) y clorhexidina (CHX) líquidos mexclados con MTA- Angelus@ y cemento Portland blanco (CBP) interfieren negativamente en el tejido subcutáneo de ratas. Material y Métodos: Se utilizaron 21 ratas tipo wistar (rattus novergicus) que se clasificaron en cuatro grupos experimentales, a los que se les implantaron 4 tubos de de dentina rellenos de MTA+CaCl2, MTA+-CHX, CPB+CaCl2 y CPB+CHX. Los animales fueron sacrificados a los 7,15 y 30 días por sobredosis, obteniéndose cortes microscópicos seriados de 5µm, tiñéndose con hematoxilina y eosina. Resultados: La prueba estadística de X2 de Pearson demostró que entre los grupos experimentales existió diferencia estadísticamente significativa, en los linfocitos a los 7 días, células gigantes multinucleadas a los 7 y 15 días, edema a los 7 días, proliferación fibroblastica y angioblástica a los 7 días. Conclusiones: Se concluye que los aditivos utilizados en ambos materiales no interfirieron respecto a la biocompatibilidad, por lo que sería factible su indicación en determinadas situaciones clínicas (AU)


Objective: The purpose of this study was to determine if the addiction of calcium chloride (CaCl2) and chlorhexidine (CHX) mixed the liquids with MT-Angelus@ and white Portland cement (CBP) do not interfere negatively in rats subcutaneous tissue. Material and Methodology: Twenty-one Wistar type rats (rattus novergicus) were used and were classified three experimental groups and one control group, to which the implanted 4 tubes of dentin filld with MTA+CaCl2, MTA+-CHX, CPB+CaCl2 y CPB+CHX. The animals were sacrifices by overdose on the 7th, 15th and the 30th day, getting microscopic shavings having the serial 5µn, dying its color with hematoxylin and cosin. Results: Pearson´s statistical X2 test showed that there was a significant statistical difference between the experimental groups, in the lymphocytes at 7 days, CGMi at 7 and 15 days, edema after 7 days, fibroblasts and angioblastical proliferation at 7 days. Conclusions: It is therefore concluded that the additives used in both materials do not interfere with the biocompatibility; they may be noticeable in clinical situations (AU)


Assuntos
Animais , Clorexidina/uso terapêutico , Cimentos Dentários/análise , Cloreto de Cálcio/uso terapêutico , Teste de Materiais/métodos , Tela Subcutânea , Ratos , Materiais Biocompatíveis/análise
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