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1.
Int. microbiol ; 22(3): 369-376, sept. 2019. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-184844

RESUMO

Escherichia coli cells have been observed earlier to display caspase-3-like protease activity (CLP) and undergo programmed cell death (PCD) when exposed to gamma rays. The presence of an irreversible caspase-3 inhibitor (Ac-DEVD-CMK) during irradiation was observed to increase cell survival. Since radiation is known to induce SOS response, the effect of a caspase-3 inhibitor on SOS response was studied in E. coli. UV, a well-known SOS inducer, was used in the current study. Cell filamentation in E. coli upon UV exposure was found to be inhibited by ninefold in the presence of a caspase-3 inhibitor. CLP activity was found to increase twofold in UV-exposed cells than in control (non-treated) cells. Further, bright fluorescing filaments were observed in UV-exposed E. coli cells treated with FITC-DEVD-FMK, a fluorescent dye tagged with an irreversible caspase-3 inhibitor (DEVD-FMK), indicating the presence of active CLP in these cells. Unlike caspase-3 inhibitor, a serine protease inhibitor, phenylmethanesulfonyl fluoride (PMSF), was not found to improve cell survival after UV treatment. Additionally, a SOS reporter system known as SIVET (selectable in vivo expression technology) assay was performed to reconfirm the inhibition of SOS induction in the presence of caspase-3 inhibitor. SIVET assay is used to quantify cells in which the SOS response has been induced leading to a scorable permanent selectable change in the cell. The SIVET induction frequency (calculated as the ratio of SIVET-induced cells to total viable cells) increased around tenfold in UV-exposed cultures. The induction frequency was found to decrease significantly to 51 from 80% in the cells pre-incubated with caspase-3 inhibitor. On the contrary, caspase-3 inhibitor failed to improve cell survival of E. coli ΔrecA and E. coli DM49 (SOS non-inducible) cells post UV treatment. Summing together, the results indicated a possible linkage of SOS response and the PCD process in E. coli. The findings also indicated that functional SOS pathway is required for CLP-like activity; however, the exact mechanism remains to be elucidated


No disponible


Assuntos
Inibidores de Caspase/metabolismo , Inibidores de Cisteína Proteinase/metabolismo , Escherichia coli/efeitos dos fármacos , Escherichia coli/efeitos da radiação , Viabilidade Microbiana/efeitos dos fármacos , Viabilidade Microbiana/efeitos da radiação , Raios Ultravioleta , Caspase 3/metabolismo , Escherichia coli/enzimologia , Escherichia coli/crescimento & desenvolvimento , Resposta SOS em Genética/efeitos dos fármacos , Resposta SOS em Genética/efeitos da radiação , Inibidores de Serino Proteinase/metabolismo
3.
Med. clín (Ed. impr.) ; 151(8): 299-307, oct. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-173999

RESUMO

Background: Numerous studies have evaluated the use of ultraviolet-C devices for terminal disinfection in hospitals, however, to date there is little information about the device's final impact on patients. We investigated the effect of an ultraviolet air sterilizer (UVAS) on the clinical outcomes of cardiac surgery patients. Materials and methods: This random, prospective and non-interventional study included 1097 adult patients undergoing elective cardiac surgery: 522 stayed in an ICU room with UVAS (Medixair®) and 575 patients ICU room without UVAS and were used as a control. The primary outcome measure was to evaluate the effect of a UVAS on the overall prevalence of nosocomial infections in postoperative cardiac patients in ICUs. Results: No significant differences in ventilator-associated pneumonia (4.6% vs. 5.0%, p=0.77) and total infection (14.0% vs. 15.5%, p=0.45) rates were detected in patients with and without the UVAS. The length of stay in the intensive care unit and at the hospital was similar in both groups, UVAS (4.6 (8.2) days and 18.3 (5.5) days) and without UVAS (4.6 (7.3) days and 19.2 (18.6) days). The 30-day in-hospital mortality rate was 5.3%, no significant differences between groups were observed (p=0.053). Conclusion: Novel ultraviolet-C technology has not been shown to significantly reduce nosocomial infections or mortality rates in cardiac surgery patients


Introducción: Numerosos estudios han evaluado el uso de dispositivos ultravioleta C para la desinfección terminal en hospitales; sin embargo, hasta la fecha existen pocos datos sobre el impacto en los pacientes. Hemos evaluado el efecto de un esterilizador de aire ultravioleta (UVAS) sobre las variables clínicas de los pacientes operados de cirugía cardíaca. Materiales y métodos: Este estudio prospectivo, aleatorizado no intervencional incluyó 1.097 pacientes adultos intervenidos de cirugía cardíaca electiva: 522 ingresaron en una habitación de UCI con UVAS (Medixair®) y 575 pacientes en habitación de UCI sin UVAS se utilizaron como control. La variable principal medida fue el efecto del UVAS sobre la prevalencia global de infecciones en el postoperatorio de cirugía cardíaca en UCI. Resultados: No hubo diferencias significativas en la neumonía asociada a ventilación mecánica (4,6% vs. 5,0%, p=0,77) e índices totales de infección (14,0% vs. 15,5%, p=0,45) detectados en pacientes con y sin UVAS. La duración del ingreso en UCI y en el hospital fue similar en ambos grupos, UVAS (4,6 [8,2] días y 18,3 [5,5] días) y sin UVAS (4,6 [7,3] días y 19,2 [18,6] días). La mortalidad a 30 días hospitalaria fue del 5,3%, sin diferencias significativas entre los grupos (p=0,053). Conclusión: La nueva tecnología ultravioleta C no ha demostrado disminuir las infecciones nosocomiales ni la mortalidad en pacientes de cirugía cardíaca


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Cirurgia Torácica/instrumentação , Raios Ultravioleta , Unidades de Cuidados Coronarianos , Desinfecção/instrumentação , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/mortalidade , Cuidados Pós-Operatórios , Desinfecção/métodos , Estudos Prospectivos
4.
SEMERGEN, Soc. Esp. Med. Rural Gen. (Ed. Impr.) ; 44(6): 420-429, sept. 2018. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-181235

RESUMO

Objetivos: El cáncer cutáneo (melanoma y no melanoma) es el tumor más frecuente del ser humano, siendo el melanoma el más agresivo. Dado que la exposición a la radiación ultravioleta (UV) es el único factor etiológico modificable, la fotoprotección es una medida preventiva primordial. Con estos fundamentos, se realizó una investigación cuyo objetivo principal fue comparar tres grupos poblacionales con distintos niveles de conocimiento sobre fotoprotección para conocer si existen diferencias en hábitos de exposición solar y grado de concienciación de los efectos perjudiciales del sol sobre la piel. Material y métodos: Estudio observacional transversal realizado mediante una encuesta entre octubre y diciembre de 2015 a 317 universitarios españoles con edades entre 18 y 25años. Se emplearon medidas estadísticas descriptivas y el test de chi-cuadrado. Resultados: Se realizaron seis preguntas para evaluar los hábitos de exposición solar. Se detectaron diferencias significativas en dos de ellas: autoexamen periódico de lunares (p<0,001) y empleo de factor de protección adecuado (p=0,025). Respecto al nivel de conocimientos, se realizaron cinco preguntas, encontrándose diferencias en todos los casos (p<0,001). Conclusiones: Tener más conocimientos sobre los riesgos de la exposición solar solo mejoró dos de los seis hábitos cuestionados sobre exposición solar. Probablemente existen otros factores que influyen en esta conducta, como los factores estéticos o socioculturales. La prevención temprana del cáncer de piel es fundamental, y es necesario establecer programas de promoción de la salud que tengan en cuenta estos otros condicionantes


Objectives: Skin cancer (melanoma and non-melanoma) is the most common cancer in humans, with melanoma being the most aggressive. Due to the fact that ultraviolet (UV) radiation exposure is the only adjustable aetiological factor, UV protection is the essential preventive measure. Based on these grounds, a study was conducted in order to compare three population groups with different levels of knowledge about UV protection, as well as to determine any differences in sun exposure habits, and the level of awareness of the damaging effects of the sun on the skin. Materials and methods: An observational, cross-sectional study was conducted using a questionnaire survey of 317 Spanish university students aged 18-25years old, between October and December 2015. Descriptive statistics methods and Pearson's Chi-squared were used. Results: Six questions were used to evaluate the sun exposure habits. Significant differences were detected in two of them: mole self-assessment (P<.001) and the use of an appropriate sun protection factor (P=.025). Five questions were asked concerning the level of knowledge about UV protection, with differences (P<.001) being found in all cases. Conclusions: Despite having more knowledge about the risks of sun exposure in the medical dermatology students group, only two of the six habits questioned were improved. Other factors that could be involved are the aesthetic or sociocultural factors. The early prevention of skin cancer is essential, with health promotion programs needing to be established that take into account all these conditions


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Conhecimentos, Atitudes e Prática em Saúde , Melanoma/prevenção & controle , Neoplasias Cutâneas/prevenção & controle , Estudantes de Medicina/psicologia , Atividade Solar , Raios Ultravioleta/efeitos adversos , Estudos Transversais , Hábitos , Comportamentos Relacionados com a Saúde , Educação em Saúde/métodos , Promoção da Saúde , Inquéritos e Questionários
7.
Med. oral patol. oral cir. bucal (Internet) ; 23(1): e7-e12, ene. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-170297

RESUMO

Background: Lower lip squamous cell carcinoma (LLSCC) is a common malignancy of the head and neck, being mainly a consequence of a chronic exposure to ultraviolet (UV) light solar radiation. Here, we evaluated the clinicopathological profile of patients with photosensitive disorders (xeroderma pigmentosum, lupus erythematosus and albinism) that developed LLSCC. Material and Methods: Data from patients who had a diagnosed LLSCC with a prior xeroderma pigmentosum, lupus erythematosus or albinism diagnosis that were treated at INCA from 1999 to 2012 were collected from patients' medical records (n=16). The control group was composed of 68 patients with LLSCC without a medical history of photosensitivity. The clinicopathological data of this study population were collected and the association between these variables was analyzed by Fisher's exact test. Survival curves were constructed using the Kaplan-Meier method and compared by log-rank test. All statistical analyses were performed using SPSS statistics package. Results: The mean age of patients in the photosensitive and non-photosensitive groups was 42 years and 67 years, respectively (p<0.0001). A previous history of malignant diseases was more common in the photosensitive group (p=0.001). In both groups, most tumors showed a pathological stage I/II disease. Overall and cancer-specific survival were not statistically different. However, disease-free interval showed a significant difference (p=0.01) between the photosensitive and non-photosensitive patients. Conclusions: Photosensitive patients presented LLSCC at earlier age but it usually was not the primary tumor in these patients. Furthermore, a more aggressive pathological behavior was not seen when compared with tumors from non-photosensitive patients. The disease-free interval was lower in photosensitive patients, as expected (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Neoplasias Labiais/complicações , Carcinoma de Células Escamosas/complicações , Transtornos de Fotossensibilidade/diagnóstico , Xeroderma Pigmentoso/diagnóstico , Albinismo/diagnóstico , Transtornos de Fotossensibilidade/terapia , Raios Ultravioleta/efeitos adversos , Radiação Solar/efeitos adversos , Estimativa de Kaplan-Meier
8.
Actas dermo-sifiliogr. (Ed. impr.) ; 108(9): 852-858, nov. 2017. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-168143

RESUMO

Introducción y objetivos: La dosis de inicio en la fototerapia UVB de banda estrecha (UVBBE) viene determinada por el fototipo o por la determinación de la dosis eritematógena mínima (DEM). El cálculo de la DEM identifica pacientes con fotosensibilidad no sospechada. El objetivo de nuestro estudio es conocer la influencia que puedan tener en una DEM disminuida los fármacos fotosensibilizantes concomitantes, el diagnóstico, la asociación con acitretina en pacientes con psoriasis y evidenciar si la DEM alterada provoca más reacciones adversas y más graves. Material y métodos: Se trata de un estudio observacional de una cohorte retrospectiva desde el período comprendido entre el 1 de febrero de 2009 al 31 de marzo de 2015. La determinación de la DEM se categorizó en DEM normal o patológica. Resultados: Trescientos dos pacientes con distintas dermatosis inician UVBBE en función de la DEM. No se han encontrado diferencias entre el grupo con DEM patológica respecto al normal, ni en el número de fármacos consumidos (p = 0,071), ni en el potencial fotosensibilizante (p = 0,806). El análisis multivariante ajustado por edad, sexo y fototipo reveló que la psoriasis es un factor protector de DEM patológica (OR = 0,31 [IC 95%: 0,16-0,58]). No se encontró riesgo significativo de eritema ni prurito en los pacientes con DEM alterada OR = 1,68 (IC 95%: 0,91-3,29) y OR = 2,04 (IC 95%: 0,99-4,22), respectivamente. Conclusiones: La psoriasis protege de tener una DEM patológica. Si bien el eritema y el prurito fueron más frecuentes en los pacientes con DEM patológica, las diferencias no fueron estadísticamente significativas (AU)


Introduction and objectives: The starting dose for narrowband UV-B phototherapy is determined by a patient's skin phototype or minimal erythema dose (MED). Calculation of MED identifies patients with unsuspected photosensitivity. The aim of this study was to investigate the influence of factors such as concomitant use of photosensitizing agents, diagnosis, and combination with acitretin in patients with psoriasis on the frequency and severity of adverse effects in patients with a low MED to narrowband UV-B phototherapy. Material and methods: We undertook a retrospective observational cohort study between February 1, 2009 and March 31, 2015. MED values were classified as normal or low. Results: In total, 302 patients with different skin conditions started narrowband UV-B phototherapy at a dose determined by their MED. No differences were found between patients with a low MED and those with a normal MED for number of drugs taken (P = .071) or use of photosensitizing agents (P = 0.806). Following adjustment for age, sex, and phototype, the multivariate analysis showed that psoriasis exerted a protective effect against a low MED (OR = 0.31 [95% CI, 0.16-0.58]). No significant risk of erythema or pruritus was detected in patients with a low MED (OR = 1.68; 95% CI, 0.91-3.29 and OR = 2.04; 95% CI, 0.99-4.22, respectively). Conclusions: Psoriasis protects against a low MED. Although erythema and pruritus were more common in patients with a low MED, the differences were not significant (AU)


Assuntos
Humanos , Eritema/prevenção & controle , Terapia Ultravioleta/métodos , Dermatopatias/terapia , Fototerapia/métodos , Doses de Radiação , Transtornos de Fotossensibilidade/diagnóstico , Raios Ultravioleta/efeitos adversos , Psoríase/terapia
9.
Pediatr. catalan ; 77(3): 97-99, jul.-sept. 2017. ilus
Artigo em Catalão | IBECS | ID: ibc-168784

RESUMO

Introducció: les fitofotodermatitis són les reaccions cutànies induïdes pel contacte de certs vegetals amb la pell sumades a l'efecte de la radiació ultraviolada. Les substàncies que hi estan implicades més sovint solen ser les furocumarines, presents en rutàcies (llimoner, taronger i llima), moràcies (figuera) i umbel•líferes (pastanaga, api i julivert), entre altres. Aquestes reaccions es manifesten amb plaques eritematoses urticariformes sobres les quals es poden arribar a formar vesícules i butllofes, simulant una cremada. Típicament, el contacte amb les plantes ocasiona lesions cutànies amb patrons lineals. Aquest treball permet desenvolupar les característiques clíniques de l'entitat partint d'un cas particular. Cas clínic: un nen de 9 anys va acudir a urgències per una placa eritematosa a la cama esquerra sobre la qual havien aparegut butllofes i en què s'intuïa una disposició lineal. Dos dies abans havia estat jugant al camp exposat al sol i amb pantalons curts. El pacient va ser hospitalitzat i es va procedir a la flictenectomia de les ampolles tenses més grans, a l'administració d'analgèsics, corticoides i antihis-tamínics per via endovenosa, i a cures amb foments de permanganat potàssic i crema de sulfadiazina argèntica. L'evolució va ser favorable i el nen va poder continuar amb les cures de forma ambulatòria. Revisant les plantes presents al camp que va visitar el pacient trobem algun exemplar d'umbel•lífera.Comentaris: la fitofotodermatitis és una condició infreqüent que generalment no suposa gravetat, però les lesions ampul•lars locals poden ser molt cridaneres ja que semblen una cremada. El diagnòstic és fonamentalment clínic, i és imprescindible mantenir un alt grau de sospita


Introducción. Las fitofotodermatitis son aquellas reacciones cutáneas inducidas por el contacto de ciertos vegetales con la piel sumadas a la radiación ultravioleta. Las sustancias más frecuentemente implicadas suelen ser furocumarinas, presentes en rutáceas (limonero, naranjo y lima), moráceas (higuera) y umbelíferas (zanahoria, apio y perejil), entre otras. Dichas reacciones se manifiestan con placas eritematosas urticariformes sobre las que pueden llegar a formarse vesículas y ampollas, simulando una quemadura. Típicamente, el contacto con las plantas ocasiona lesiones cutáneas con patrones lineales. Este trabajo permite desarrollar las características clínicas de la entidad partiendo de un caso particular. Caso clínico. Un niño de 9 años acudió a urgencias por una placa eritematosa en la pierna izquierda sobre la que habían aparecido ampollas, y en la que se intuía una disposición lineal. Dos días antes había estado jugando en el campo y, con pantalón corto, había estado también expuesto al sol. El paciente fue hospitalizado y se procedió a la flictenectomía de las ampollas tensas de mayor tamaño, a la administración de analgésicos, corticoides y antihistamínicos por vía endovenosa, y a curas con fomentos de permanganato potásico y crema de sulfadiazina argéntica. La evolución fue favorable y el niño pudo continuar con las curas de forma ambulatoria. Revisando las plantas presentes en el campo que visitó el paciente encontramos algún ejemplar de umbelífera. Comentarios. La fitofotodermatitis es una condición infrecuente que generalmente no supone gravedad, pero las lesiones ampollosas locales pueden ser muy llamativas simulando una quemadura. El diagnóstico es fundamentalmente clínico, siendo imprescindible mantener un alto grado de sospecha (AU)


Introduction. Phytophotodermatitis is a skin reaction occurring after contact of the skin with a plant in conjunction with exposure to UV irradiation. The substances most frequently involved are furocoumarines, which are usually present in Rutaceae (lemon, orange, and lime), Moraceae (fig), and Umbelliferae (carrot, celery, and parsley), among others. Such reactions manifest by erythematous urticarial plaques that can develop vesicles and blisters, simulating a burn. Contact with the plants usually causes skin lesions with linear patterns. In this report we present a clinical characterization of this condition. Case report. A 9 years old boy presented to the Emergency Department with an erythematous plaque and blisters in a linear pattern in his left leg. Two days earlier he had been playing in a field wearing shorts and exposed to the sun. The patient was admitted, the large blisters were drained and treated with potassium permanganate soaks and silver sulfadiazine cream, and he received intravenous analgesics, corticosteroids, and antihystaminics. The outcome was good and topical treatments were continued as outpatient. Evaluation of the plants in the playing field revealed the presence of Umbelliferae. Comments. Phytophotodermatitis is not a common or severe disease, but bullous lesions can be striking and mimic a burn. Diagnosis is clinical, and a high level of suspicion is required (AU)


Assuntos
Humanos , Masculino , Criança , Transtornos de Fotossensibilidade/diagnóstico , Dermatite Alérgica de Contato/diagnóstico , Raios Ultravioleta/efeitos adversos , Plantas/efeitos adversos , Urticária/etiologia
10.
Actas dermo-sifiliogr. (Ed. impr.) ; 108(5): e33-e37, jun. 2017. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-163790

RESUMO

We present a series of 6 cases of disseminated superficial actinic porokeratosis and describe their dermoscopic features. Dermoscopy is a noninvasive in vivo technique that is useful as a tool for the diagnosis and follow-up of porokeratosis. This condition has specific dermoscopic features that were observed in our series of cases and that are consistent with reports in the international literature (AU)


Presentamos una serie de 6 casos de poroqueratosis actínica superficial diseminada en los cuales se describen las características dermatoscópicas halladas en cada uno de ellos. La dermatoscopia es una técnica no invasiva in vivo útil como herramienta diagnóstica y de seguimiento en la poroqueratosis ya que se describen elementos dermatoscópicos específicos de esta entidad observados en nuestra serie de casos, que concuerdan con lo comunicado en la literatura internacional (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Poroceratose/diagnóstico , Dermoscopia/métodos , Raios Ultravioleta/efeitos adversos , Transtornos de Fotossensibilidade/diagnóstico , Biópsia
14.
Med. oral patol. oral cir. bucal (Internet) ; 21(1): e53-e58, ene. 2016. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-149425

RESUMO

Background: Actinic prurigo (AP) is an idiopathic photodermatosis, this entity requires exposure to UV-B and -A to develop lesions. Apoptosis is a physiological death program that can be initiated by a permanently active mechanism (extrinsic pathway) or irreparable damage (intrinsic pathway). Material and methods: Descriptive study, the sample size comprised 64 paraffin blocks of tissue with a diagnosis of AP. In H&E-stained slides, the diagnosis of AP was corroborated, and 1-µm-thick sections were processed for immunohistochemistry (IHC). A database was constructed with SPSS version 20, Inc., Chicago, IL, USA, and descriptive statistics were analyzed by X2 test and comparison of means. Results: A total of 64 cases were processed, of which 40 (62.5%) were cheilitis AP and 24 (37.5%) were AP in the skin. Of the 40 cheilitis samples, 27 were positive for Bcl-2 and caspase 3 (67.5%), p53 was expressed in 30 (75%). Of the skin lesions, p53 and caspase 3 were expressed in 18 of 24 cases (75%), and 13 were positive for Bcl-2 (54%). Conclusions: We propose that apoptosis is the last step in the type IV subtype a-b hypersensitivity responseactivation of the intrinsic pathway indicates that external factors, such as UV-A and -B are the trigger (AU)


No disponible


Assuntos
Humanos , Apoptose/fisiologia , Prurigo/etiologia , Ceratose Actínica/diagnóstico , Queilite/diagnóstico , Diagnóstico Diferencial , Raios Ultravioleta/efeitos adversos
19.
Actas dermo-sifiliogr. (Ed. impr.) ; 105(8): 780-788, oct. 2014. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-128815

RESUMO

ANTECEDENTES: La técnica del fototest evalúa la sensibilidad de la piel a la radiación ultravioleta (RUV) mediante la determinación de la mínima dosis de radiación capaz de producir eritema (dosis mínima eritemática [DEM]) y la respuesta anómala a UVA. No existen guías protocolizadas para la técnica del fototest. METODOLOGÍA: Estudio multicéntrico de cohortes prospectivo. Un total de 232 voluntarios sanos fueron reclutados en 9 centros hospitalarios. El fototest se realizó con simuladores solares (SS) o lámparas fluorescentes de UVB de banda ancha (UVBBA). Cada sujeto recibió un total de 5 o 6 dosis progresivas de radiación eritemática y 4 dosis de UVA. La lectura se realizó a las 24 h. RESULTADOS: La DEM media por fototipo fue de 23 ± 8, 28 ± 4, 35 ± 4 y 51 ± 6 mJ/cm2 (fototipos I a IV respectivamente) para los centros que utilizaron SS y de 28 ± 5, 32 ± 3 y 34 ± 5 mJ/cm2 cuando se utilizaron lámparas de UVBBA para fototipos del II al IV. Se consideraron valores de DEM patológica 7, 19, 27 y 38 mJ/cm2, para los fototipos I al IV respectivamente cuando se emplearon SS y de 18, 24 y 24 mJ/cm2 para los fototipos II-IV expuestos a lámparas de UVBBA. A dosis de hasta 20 J/cm2 de UVA no se observaron respuestas anómalas. CONCLUSIONES: Existe homogeneidad de resultados en los diferentes centros participantes, lo que permite estandarizar el método del fotodiagnóstico para los diferentes fototipos cutáneos, así como establecer las dosis umbral que definen una respuesta anómala a la radiación ultravioleta


BACKGROUND: Phototesting is a technique that assesses the skin's sensitivity to UV radiation by determining the smallest dose of radiation capable of inducing erythema (minimal erythema dose [MED]) and anomalous responses to UV-A radiation. No phototesting protocol guidelines have been published to date. METHODOLOGY: This was a multicenter prospective cohort study in which 232 healthy volunteers were recruited at 9 hospitals. Phototests were carried out with solar simulators or fluorescent broadband UV-B lamps. Each individual received a total of 5 or 6 incremental doses of erythemal radiation and 4 doses of UV-A radiation. The results were read at 24 hours. RESULTS: At hospitals where solar simulators were used, the mean (SD) MED values were 23 (8), 28 (4), 35 (4), and 51 (6) mJ/cm2 for skin phototypes I to IV, respectively. At hospitals where broadband UV-B lamps were used, these values were 28 (5), 32 (3), and 34 (5) mJ/cm2 for phototypes II to IV, respectively. MED values lower than 7, 19, 27, and 38 mJ/cm2 obtained with solar simulators were considered to indicate a pathologic response for phototypes I to IV, respectively. MED values lower than 18, 24, and 24 mJ/cm2 obtained with broadband UV-B lamps were considered to indicate a pathologic response for phototypes II to IV, respectively. No anomalous responses were observed at UV-A radiation doses of up to 20 J/cm2. CONCLUSIONS: Results were homogeneous across centers, making it possible to standardize diagnostic phototesting for the various skin phototypes and establish threshold doses that define anomalous responses to UV radiation


Assuntos
Humanos , Masculino , Feminino , Raios Ultravioleta/efeitos adversos , Raios Ultravioleta , Raios Ultravioleta/classificação , Pele/patologia , Pele/efeitos da radiação
20.
Index enferm ; 23(3): 144-148, jul.-sept. 2014. tab
Artigo em Espanhol | IBECS | ID: ibc-130253

RESUMO

Objetivo principal: La higiene de manos (HM) es fundamental para una atención sanitaria limpia y segura. Los futuros profesionales de enfermería deben adquirir conocimientos tanto teóricos como prácticos en materia de HM. Metodología: Se evaluó la técnica de HM, registrando las regiones de las manos con/sin exposición a la solución alcohólica marcada con fluoresceína mediante una lámpara de luz ultravioleta. Resultados principales: Evaluamos 71 alumnos, 56,3 % de 2o curso y 43,7% de 3er curso de Enfermería. El 73,3% fueron mujeres. Realizaron la HM bien o muy bien el 45,1% de los alumnos. Las zonas donde con más frecuencia no se extendió la solución alcohólica fueron los pulgares (54,9%) y dorso (46,5%). No hubo diferencias significativas entre los dos cursos. Conclusión principal: El papel de la educación es fundamental para sentar las bases de buenas prácticas en HM, en conocimientos teóricos y en el desarrollo de habilidades y reforzamiento de buenas prácticas


Objective: Hand hygiene (HH) is essential for a clean and safe healthcare. The future nurses have to acquire both, theoretical and practical, knowledge, competencies and skills on HH. Methods: The HH technique was evaluated using an alcoholic solution incorporating fluorescent marker and ultraviolet lamp, and recording regions of the hands with / without exposure. Results: We evaluated 71 students, 56.3% in the 2nd year and 43.7% of 3rd year of Nursing School. The 73.3% were women. The HH was performed very good or good by 45.1% of students. The areas that alcoholic solution didn't reached most frequently were thumbs (54.9%) and dorsal part of hand (46.5%). No significant differences between courses were found. Conclusions: The role of education is essential to lay the foundation of good technique in HH, in theoretical knowledge and in the development of skills and reinforcement of good practices


Assuntos
Humanos , Infecção Hospitalar/prevenção & controle , Desinfecção das Mãos/normas , Assepsia/normas , Raios Ultravioleta , Estudantes de Enfermagem/estatística & dados numéricos , Higienizadores de Mão/análise
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