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1.
Endodoncia (Madr.) ; 37(2): 22-28, sept. 2019. graf, ilus
Artigo em Espanhol | IBECS | ID: ibc-186296

RESUMO

Objetivos. El propósito de este trabajo fue evaluar los efectos biológicos de MTA Repair HP y ProRoot MTA en células madre procedentes de ligamento periodontal (hPDLSCs) tras ser ex-puestos los cementos a ambiente ácido y neutro. Material y Métodos: Discos de cada material (n=30) fueron ex-puestos a un tampón fosfato-salino (pH 7.4) o a ácido butírico (pH 5.2) durante 7 días, posteriormente se realizaron pruebas biológicas in vitro usando hPDLSCs. A partir de eluatos de los diferentes materiales de obturación a retro, se analizaron pruebas de viabilidad celular y apoptosis. Para evaluar la adhesión celular, hPDLSCs se sembraron directamente sobre la superficie de los materiales y se observaron bajo microscopio electrónico de barrido. Para analizar estadísticamente los resultados se usaron los test ANOVA y Tukey test (p < 0.05). Resultados: Los cementos endodónticos expuestos a ambiente ácido mostraron un similar grado de adhesión celular, y sorprendentemente, MTA Repair HP a pH 5.2 exhibió una mayor viabilidad celular que ProRootMTA (p<0.05). A pH 7.4, ProRooT MTA obtuvo una tasa mayor de viabilidad celular que con MTA Repair HP. Conclusiones: Los materiales ProRoot MTA y MTA Repair HP presentaron adecuadas propiedades biológicas en los diferentes ambientes, en términos de viabilidad celular, apoptosis y adhesión


Objectives: The purpose of this work was to evaluate the biolo-gical effects of MTA Repair HP and ProRoot MTA on stem cells from periodontal ligament (hPDLSCs) after exposure to acidic and neutral environments. Material and Methods: Discs of each material (n=30) were ex-posed to phosphate buffered saline (pH = 7.4) or butyric acid (pH = 5.2) for 7 days, and biological testing was carried out in vitro on hPDLSCs. Cell viability and apoptosis assays were performed using eluates of each root-end filling material. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. Statistical differences were assessed by ANOVA and Tukey test (p < 0.05).Results: Endodontic cements exposure to an acidic environment showed a similar degree of cell adherence, and, surprisingly, MTA Repair HP exhibited higher cell viability rates at pH 5.2 than Pro-Root MTA, whereas ProRoot MTA 7.4 showed higher cell viability rates than MTA Repair HP. Conclusions: Adequate biological properties of ProRoot MTA and MTA Repair HP in terms of cell viability, cell death and cell attach-ment were observed in both environments


Assuntos
Humanos , Adolescente , Adulto Jovem , Adulto , Cimentos Dentários/uso terapêutico , Concentração de Íons de Hidrogênio , Ligamento Periodontal , Células-Tronco , Reações Biológicas , Separação Celular , Sobrevivência Celular , Apoptose , Materiais Restauradores do Canal Radicular/classificação , Materiais Restauradores do Canal Radicular/uso terapêutico , Obturação do Canal Radicular/métodos
2.
Int. microbiol ; 22(3): 355-361, sept. 2019. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-184842

RESUMO

The effect of oxygen on anaerobic protozoa was studied in anaerobic batch reactors inoculated with sludge and protozoa cultures. Among the protozoa genera, Metopus, Brachonella, Plagiopyla, Trepomonas, and Vanella were more sensitive to oxygen compared to other genera. Protozoa genera Menoidium, Rhynchomonas, Cyclidium, Spathidium, and Amoeba were found to survive under aerobic conditions, and the growth rate was slightly higher or similar to anaerobic condition. O2 tension resulted in the loss of free and endosymbiotic methanogens in anaerobic system, while methanogens were observed inside the protozoan cysts. Survival of anaerobic protozoa declined considerably when the O2 tension exceeded 1% atm. sat. and showed chemosensory behavior in response to O2 exposure. Superoxide dismutase activity was detected in survived protozoa cells under O2 tension. Facultative anaerobic protozoa with SOD activity can provide a mechanism to overcome possible occurrence of oxygen toxicity in the treatment of wastewater in anaerobic reactor


No disponible


Assuntos
Amoeba/efeitos dos fármacos , Cilióforos/efeitos dos fármacos , Meios de Cultura/química , Euglênidos/efeitos dos fármacos , Kinetoplastida/efeitos dos fármacos , Oxigênio/toxicidade , Aerobiose , Amoeba/crescimento & desenvolvimento , Amoeba/metabolismo , Anaerobiose , Reatores Biológicos/parasitologia , Cilióforos/crescimento & desenvolvimento , Cilióforos/metabolismo , Euglênidos/crescimento & desenvolvimento , Euglênidos/metabolismo , Kinetoplastida/crescimento & desenvolvimento , Kinetoplastida/metabolismo , Metano/metabolismo , Sobrevivência Celular
3.
Allergol. immunopatol ; 47(2): 179-184, mar.-abr. 2019. graf, ilus, tab
Artigo em Inglês | IBECS | ID: ibc-180807

RESUMO

Introduction: Asthma is a chronic inflammatory, heterogeneous airway disease affecting millions of people around the world. Dendritic cells (DCs) are considered the most important antigen-presenting cell in asthma airway inflammatory reaction. But whether osteoprotegerin (OPG) mediate RANK/RANKL signaling inhibition influences asthma development by affecting the survival and function of DCs remains unclear. In this study, we assessed the effects of OPG on DCs and asthma. Material and methods: BALB/c mice immunized with ovalbumin (OVA) were challenged thrice with an aerosol of OVA every second day for eight days. Dexamethasone (1.0mg/kg) or OPG (50 mig/kg) was administered intraperitoneally to OVA-immunized BALB/c mice on day 24 once a day for nine days. Mice were analyzed for effects of OPG on asthma, inflammatory cell infiltration and cytokine levels in lung tissue. The expression of RANK and β-actin was detected by Western Blot. DCs were isolated from mouse bone morrow. Cell survival was assessed by cell counting. The content of IL-12 was detected by ELISA. Results: Results showed that OVA increased the number of inflammatory factors in BALF, elevated lung inflammation scores in mice. OPG reversed the alterations induced by OVA in the asthmatic mice. OPG inhibited the survival and function of DC via inhibition of RANK/RANKL signaling. Conclusions: This research proved inhibition of RANK/RANKL signaling by OPG could ease the inflammatory reaction in asthma, providing new evidence for the application of OPG on asthma


No disponible


Assuntos
Humanos , Animais , Feminino , Camundongos , Asma/metabolismo , Células Dendríticas/fisiologia , Osteoprotegerina/metabolismo , Pneumonia/metabolismo , Apresentação do Antígeno , Asma/imunologia , Sobrevivência Celular , Citocinas/metabolismo , Camundongos Endogâmicos BALB C , Pneumonia/imunologia , Ligante RANK/metabolismo , Receptor Ativador de Fator Nuclear kappa-B/metabolismo
4.
J. physiol. biochem ; 74(3): 359-367, ago. 2018. graf
Artigo em Inglês | IBECS | ID: ibc-178991

RESUMO

A large number of researches have led to a substantial growth of knowledge about exercise and oxidative stress. Initial investigations reported that physical exercise generates free radical-mediated damages to cells; however, in recent years, studies have shown that regular exercise can upregulate endogenous antioxidants and reduce oxidative damage. Yet, strenuous exercise perturbs the antioxidant system by increasing the reactive oxygen species (ROS) content. These alterations in the cellular environment seem to occur in an exercise type-dependent manner. The source of ROS generation during exercise is debatable, but now it is well established that both contracting and relaxing skeletal muscles generate reactive oxygen species and reactive nitrogen species. In particular, exercises of higher intensity and longer duration can cause oxidative damage to lipids, proteins, and nucleotides in myocytes. In this review, we summarize the ROS effects and interplay of antioxidants in skeletal muscle during physical exercise. Additionally, we discuss how ROS-mediated signaling influences physical exercise in antioxidant system


No disponible


Assuntos
Humanos , Animais , Antioxidantes/uso terapêutico , Exercício Físico , Estilo de Vida Saudável , Músculo Esquelético/metabolismo , Estresse Oxidativo , Espécies Reativas de Oxigênio/antagonistas & inibidores , Traumatismo por Reperfusão/prevenção & controle , Anti-Inflamatórios não Esteroides/metabolismo , Anti-Inflamatórios não Esteroides/uso terapêutico , Antioxidantes/metabolismo , Sobrevivência Celular , Dieta Saudável , Mitocôndrias Musculares , Fadiga Muscular , Músculo Esquelético/fisiopatologia
5.
Cient. dent. (Ed. impr.) ; 15(1): 53-60, ene.-abr. 2018. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-172861

RESUMO

La superficie de los implantes dentales es un parámetro fundamental a considerar para mejorar la regeneración ósea basada en implantes. Junto con las técnicas quirúrgicas y los materiales y herramientas empleados, las modificaciones superficiales de los implantes han ido evolucionando con el tiempo para permitir acortar los tiempos de tratamiento y abordar reconstrucciones cada vez más complejas. La superficie unicCa® resulta de la incorporación a la superficie multirrugosa optima® de una capa de iones de calcio. Las modificaciones realizadas en el desarrollo de esta nueva superficie hacen que presente ventajas como: impedimento del envejecimiento del óxido de titanio, mejora de la unión hueso-implante y aceleración de la fase de la osteointegración. Para demostrar las propiedades de esta nueva superficie se realizaron estudios en cóndilo femoral de conejo y en tibia de oveja. En ambos casos se demostró un incremento y aceleración de la osteointegración. El calcio, presente en la superficie unicCa®, asegura la estimulación celular desde los primeros momentos tras la implantación hasta la consolidación de los tejidos y la formación de la capa calcificada de osteointegración de la que es el constituyente principal. Esto implica una regeneración peri-implante más rápida y de mejor calidad


The surface of the dental implants is a fundamental parameter to consider for improving bone regeneration based on implants. Together with the surgical techniques and materials and tools used, surface modifications of the implants have evolved over time to allow to shorten treatment times and address increasingly complex reconstructions. The unicCa® surface is the result of the addition to the optima® surface of a layer of calcium ions. The modifications made in the development of this new surface make this advantages such as: impairment of the aging of the titanium oxide, improvement of the union bone-implant and acceleration of the phase of the osseointegration. To demonstrate the properties of this new area studies were performed in rabbit femoral condyle and in tibia of sheep. In both cases showed an increase and acceleration of the osseointegration. The calcium, present on the the unicCa® surface ensures the cellular stimulation from the first moments after implantation until the consolidation of the tissues and the formation of the osseointegration of calcified layer which is the main constituent. This implies a regeneration peri-implant faster and of better quality


Assuntos
Humanos , Implantação Dentária Endo-Óssea/métodos , Osseointegração , Ionóforos de Cálcio/uso terapêutico , Sobrevivência Celular/imunologia , Cálcio/farmacocinética
6.
Endocrinol. diabetes nutr. (Ed. impr.) ; 65(4): 200-205, abr. 2018. graf
Artigo em Inglês | IBECS | ID: ibc-172150

RESUMO

Introduction: Vascular endothelial growth factor (VEGF) plays an essential role in development of diabetic macular edema (DME). While there is evidence suggesting that silymarin, a flavonoid extracted from Silybum marianum, could be useful for prevention and treatment of diabetic nephropathy, no studies have been conducted in diabetic retinopathy (DR). The aim of this study was to assess the effect of silymarin on disruption of inner blood retinal barrier (BRB), the primary cause of DME. Materials and methods: Human retinal endothelial cells (HRECs) were cultured under standard (5.5mM D-glucose) and diabetogenic conditions (25mM D-glucose and 25mM D-glucose + recombinant vascular endothelial growth factor [rVEGF, 25mg/mL]). To assess cell viability, three concentrations of silymarin were tested (2, 4 and 10μg/mL). The effect of silymarin on HREC disruption was determined using a dextran (70kD) permeability asssay. Results: No differences were found in the viability of HRECs treated with 2 or 4μg/mL of silymarin as compared to untreated cells, but viability significantly decreased after using 10 μg/mL. The concentration of 4 μg/mL was therefore selected. Silymarin (4μg/mL) caused a significant decrease in VEGF-induced permeability in both media with 5.5nM (422±58 vs. 600±72 ng/mL/cm2; p<0.03) and 25nM of D-glucose (354 ± 28 vs. 567 ± 102 ng/mL/cm2; p<0.04). Discussion: Our results show that silymarin is effective for preventing hyperpermeability induced by diabetic conditions in HRECs. Further studies are needed to assess whether silymarin could be useful to treat DME (AU)


Introducción: El Vascular endothelial growth factor (VEGF) juega un papel esencial en el desarrollo del edema macular diabético (EMD). Existe evidencia que indica que el uso de la silimarina, extracto flavonoide del Silybum marianum, podría ser útil en la prevención y el tratamiento de la nefropatía diabética pero no se dispone de datos en retinopatía diabética (RD). El objetivo del estudio es evaluar el efecto de la silimarina sobre la disrupción de la barrera hematorretininana, que es la causa primaria del EMD. Material y métodos: Células endoteliales de retina humana (HRECs) se cultivaron en condiciones estándar (5.5mM de D-glucosa) y en condiciones suprafisiológicas de glucosa (25mM de D-glucosa y 25mM de D-glucosa + VEGF 25mg/dl). Para evaluar la viabilidad de las células se probaron 3 concentraciones de silimarina (2, 4 y 10μg/ml). El efecto de la silimarina sobre la disrupción de las HRECs se determinó mediante análisis de permeabilidad a dextrano (70kD). Resultados: No se observaron diferencias en la viabilidad de las HRECs tratadas con 2 o 4μg/ml de silimarina en comparación con las células no tratadas, pero se observó una reducción de la viabilidad con la concentración de 10μg/ml. Por consiguiente, se seleccionó la concentración de 4μg/ml de silimarina. La silimarina (4μg/ml) produjo un descenso significativo de la permeabilidad inducida por VEGF tanto en medio con 5.5mM de D-glucosa (422 ±58 vs. 600 ±72 ng/ml/cm2; p<0.03) como en medio con 25mM de D-glucosa (354±28 vs. 567±102 ng/ml/cm2; p<0.04). Discusión: Nuestros resultados demuestran que la silimarina es efectiva para prevenir la hiperpermeabilidad inducida por condiciones suprafisiológicas de glucosa en HRECs. Son necesarios más estudios para evaluar si la silimarina podría ser útil para el tratamiento del EMD (AU)


Assuntos
Humanos , Masculino , Feminino , Silimarina/uso terapêutico , Retinopatia Diabética/complicações , Retinopatia Diabética/dietoterapia , Degeneração Macular/dietoterapia , Edema Macular/complicações , Células Endoteliais , Dextranos/análise , Células Cultivadas , Proliferação de Células , Sobrevivência Celular , Análise de Variância
7.
Arch. med. deporte ; 35(183): 50-55, ene.-feb. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-177444

RESUMO

Introduction: Therapeutic ultrasound is one of the most used physical resources in the area of physiotherapy for the treatment of injuries. However, the multiplicity of dosimetry used in clinical practice points to its indiscriminate use for pathologies that surround skeletal muscle and expresses the limitation of the available literature on the ideal dosimetric standardization to the tissue restoration, mechanism of action and its real effects on the treatment in question. Objective: The objective of this study was to promote a systematic review about the different effects and the dosimetric parameters of therapeutic ultrasonic irradiation on the process of restoration of fibroblast cells in vitro. Methods: To select the articles, three electronic data banks were consulted, with publication from January 2000 to September 2016. The studies were tracked by three freestanding reviewers, according to inclusion and exclusion criteria. Results: 669 articles were selected and after the application of inclusion and exclusion criteria, 647 were excluded. Among the exclusions reasons there are: the utilization of another physical method, exclusive focus on another type of cell line, other experimental models or the use of another language, reaching at the end 22 studies directed to qualitative analysis. Conclusion: The results of this study showed that the scientific basis is not enough to stablish real effects and dosimetric parameters of therapeutic ultrasonic on the process of restoration of fibroblast cells in vitro, due to the lack of generalization and conflict of found results


Introducción: El ultrasonido terapéutico es uno de los recursos físicos más utilizados en el área de fisioterapia para el tratamiento de lesiones. Sin embargo, la gran cantidad de dosimetrías utilizadas en la práctica clínica muestra su uso indiscriminado para patologías que circundan el músculo esquelético y además expresa la limitación de la literatura sobre la estandarización dosimétrica ideal para la restauración del tejido, mecanismo de acción y sus efectos reales sobre el tratamiento en cuestión. Objetivos: El objetivo de este estudio fue realizar una revisión sistemática sobre los diferentes efectos y parámetros dosimétricos de la irradiación ultrasónica terapéutica en el proceso de reparación de células fibroblásticas in vitro. Material y método: Para la selección de los artículos fueron consultadas tres bases de datos para buscar publicaciones entre enero de 2000 y septiembre de 2016. La búsqueda de trabajos se realizó por tres revisores independientes, conforme a los criterios de inclusión y exclusión. Resultados: Se seleccionaron 669 artículos y tras la aplicación de los criterios de inclusión, se excluyeron 647 estudios. Entre los motivos de exclusión están la utilización de otro medio físico, enfoque exclusivo de otro tipo de línea celular, otros modelos experimentales o el uso de otro idioma, quedando 22 estudios para el análisis cualitativo. Conclusión: Los hallazgos de este estudio mostraron que la base científica todavía es insuficiente para el establecimiento de los efectos reales y parámetros dosimétricos de la irradiación ultrasónica terapéutica en el proceso de reparación de células fibroblásticas in vitro, por la falta de generalización y conflicto de los resultados encontrados


Assuntos
Dosimetria in Vivo/métodos , Fibroblastos/efeitos da radiação , Sobrevivência Celular/efeitos da radiação , Terapia por Ultrassom/métodos , 25783 , Fibroblastos/citologia , Doses de Radiação , Terapia com Luz de Baixa Intensidade/métodos
8.
Rev. esp. patol. torac ; 29(4): 216-225, dic. 2017. graf, ilus
Artigo em Espanhol | IBECS | ID: ibc-170398

RESUMO

En este trabajo usamos dióxido de titanio (TiO2), fabricado mediante nanotecnología. Para demostrar su superioridad respecto al talco, realizamos un estudio in vitro comparando la respuesta pro-inflamatoria de ambos agentes sobre células malignas y mesoteliales benignas; investigando la posible inducción de apoptosis y la posible inhibición de angiogénesis también por ambos agentes. Realizamos cultivo de líneas celulares derivadas de mesotelio humano, procedente de mesotelioma bifásico humano y adenocarcinoma bronquial humano. Las células se co-cultivaron con diferentes dosis de talco y de nanopartículas de TiO2. En todas las muestras de sobrenadantes de los cultivos se analizaron los niveles de diferentes mediadores inflamatorios. La tasa de apoptosis se analizó por la expresión de Caspasa-3. Para el estudio de angiostasis se determinaron los niveles de endostatina mediante técnica ELISA. Observamos que la viabilidad de las células mesoteliales benignas es mucho menor al emplear TiO2. En el caso de las células mesoteliales malignas, se observó el mismo efecto con dosis alta de TiO2. En el adenocarcinoma de pulmón, la viabilidad de estas células expuestas al talco fue netamente inferior a la que se observó en la línea celular benigna. La producción de IL-8 fue mucho mayor por parte de las células mesoteliales neoplásicas que por las benignas y aumentó siguiendo un patrón dosis dependiente frente al talco, mientras que cayó con el TiO2. Según estos resultados, se demuestra que el talco es superior al TiO2 en su capacidad de producir mediadores que favorecerían la pleurodesis para el control del derrame pleural maligno


For this study, we used titanium dioxide (TiO2), produced using nanotechnology. To show its superiority with respect to talc, we completed an in vitro study comparing the pro-inflammatory response of both agents towards malignant and benign mesothelial cells; researching the possible apoptosis induction and possible inhibition of angiogenesis for both agents. We took a culture of cell lines derived from human mesothelioma, originating from human biphasic mesothelioma and human bronchial adenocarcinoma. The cells were cocultured with different doses of talc and TiO2 nanoparticles. The levels of different inflammatory mediators were analyzed for each culture supernatant sample. The apoptosis rate was analyzed using caspase-3 expression. The endostatin levels were determined for the angiostasis study using the ELISA technique. We observed that the viability of the benign mesothelial cells is much lower after using TiO2. In the case of malignant mesothelial cells, the same effect was observed with a high dose of TiO2. In adenocarcinoma of the lung, the viability of these cells exposed to talc was distinctly lower than that which was observed in the benign cell line. IL-8 production was much higher in neoplastic mesothelial cells than in benign cells and increased following a dose-dependent pattern with talc, while it decreased with TiO2. According to these results, we can see that talc is superior to TiO2 in its ability to produce mediators which favor pleurodesis for the control of malignant pleural effusions


Assuntos
Humanos , Titânio/uso terapêutico , Nanotecnologia/métodos , Talco/uso terapêutico , Derrame Pleural/prevenção & controle , Indutores da Angiogênese/uso terapêutico , Nanopartículas/análise , Células Epiteliais , Técnicas In Vitro/métodos , Apoptose , Endostatinas/análise , Derrame Pleural/terapia , Ensaio de Imunoadsorção Enzimática/métodos , Pleurodese/métodos , Sobrevivência Celular , Epitélio
9.
Med. oral patol. oral cir. bucal (Internet) ; 22(5): e651-e659, sept. 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-166662

RESUMO

Background: The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish. Material and Methods: An in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours. Results: A statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group). Conclusions: In comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it (AU)


No disponible


Assuntos
Humanos , Calcificação Fisiológica , Osteoblastos , Proteína Morfogenética Óssea 2/farmacocinética , Técnicas In Vitro , Estudos Prospectivos , Proteínas do Citoesqueleto/fisiologia , Apoptose/fisiologia , Matriz Óssea/crescimento & desenvolvimento , Sobrevivência Celular/fisiologia
10.
J. physiol. biochem ; 73(3): 371-380, ago. 2017. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-178888

RESUMO

A series of protective responses could be evoked to achieve compensatory adaptation once cardiomyocytes are subjected to chronic hypoxia. MLK3/JNK/c-jun signaling pathway was previously demonstrated to be involved in this process. In the present study, we aim to further examine the performance of MLK3 in hypoxic H9C2 cells and potential mechanism. Myocardial samples of patients with congenital heart disease (CHD) were collected. H9C2 cells were cultured in hypoxic conditions for various durations. MLK3 was silenced by transfection of shRNA to evaluate its role in cell viability. We found expression of MLK3 protein was lower in patients with cyanotic CHD. In hypoxic H9C2 cells, its expression was gradually decreased in a time-dependent manner. However, there was no significant difference about expression of MLK3 mRNA. According to the results of MTT, LDH, and TUNEL, faster cell growth curve, lower death rate, and less apoptotic cells could be observed in MLK-shRNA group compared with scramble-shRNA group. Silencing of MLK3 significantly reduced expression of cleaved caspase-3, cleaved PARP, Bad, and Bax, together with increased expression of Bcl-2 and ration of Bcl-2/Bax. Both ratio of phospho-JNK/total JNK and ratio of phospho-c-jun/total c-jun were significantly decreased once MLK3 was silenced. At various reoxygenation time, MLK3 shRNA could significantly promote cell survival and decrease cell death according to MTT and LDH. Our results suggested that chronic hypoxia could reduce MLK3 expression in a posttranscriptional regulatory manner. Downregulation of MLK3 protects H9C2 cells from hypoxia-induced apoptosis and H/R injury via blocking the activation of JNK and c-jun


No disponible


Assuntos
Humanos , Animais , Masculino , Feminino , Lactente , Pré-Escolar , Ratos , MAP Quinase Quinase Quinases/genética , Miocárdio/enzimologia , Adaptação Fisiológica , Proteínas Reguladoras de Apoptose/metabolismo , Hipóxia Celular , Linhagem Celular , Sobrevivência Celular , MAP Quinase Quinase Quinases/metabolismo , Sistema de Sinalização das MAP Quinases , Traumatismo por Reperfusão Miocárdica/enzimologia , Fatores de Proteção
11.
J. physiol. biochem ; 73(3): 405-414, ago. 2017. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-178892

RESUMO

Sodium butyrate (NaBu) is a by-product of microbial fermentation of dietary fiber in the gastrointestinal tract and has been shown to increase the activity of antioxidant enzymes, such as catalase or heme oxidase-1, in vivo. However, the mechanism of this effect is still unclear. This study investigated the antioxidant effect of NaBu on HepG2 cells under H2O2-induced oxidative stress. NaBu (0.3 mM) attenuated cell death and accumulation of reactive oxygen species and improved multiple antioxidant parameters in H2O2-injured HepG2 cells. NaBu inhibited glycogen synthase kinase-3 beta (GSK-3Beta) by increasing the p-GSK-3 Beta (Ser9) level and promoted nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), which increased the expression of downstream antioxidant enzymes. Together with promotion of peroxisome proliferator-activated receptor gamma coactivator 1-alpha and mitochondrial DNA copy number, NaBu modulated energy metabolism and mitochondrial function, decreasing glycolysis, increasing Beta -oxidation, and enhancing the tricarboxylic acid cycle and oxidative phosphorylation. NaBu increased mitochondrial manganese-superoxide dismutase and glutathione peroxidase activity. In conclusion, NaBu protected HepG2 cells against oxidative stress by modulating Nrf2 pathway activity and mitochondrial function


No disponible


Assuntos
Humanos , Ácido Butírico/farmacologia , Mitocôndrias/metabolismo , Estresse Oxidativo , Sobrevivência Celular , Fator 2 Relacionado a NF-E2/metabolismo , Variações do Número de Cópias de DNA , Apoptose , Ciclo do Ácido Cítrico , Citoproteção , DNA Mitocondrial/genética , Glicólise , Células Hep G2 , Peróxido de Hidrogênio/farmacologia , Mitocôndrias , Fosforilação Oxidativa , Transdução de Sinais
12.
J. physiol. biochem ; 73(2): 259-266, mayo 2017. graf
Artigo em Inglês | IBECS | ID: ibc-168482

RESUMO

The primary features of Alzheimer’s disease (AD) are extracellular amyloid plaques consisting mainly of deposits of amyloid β (Aβ) peptides and intracellular neurofibrillary tangles (NFTs). Sets of evidence suggest that interleukin-5 (IL-5) is involved in the pathogenesis of AD. Herein, we investigated the protective role of IL-5 in PC12 cells, to provide new insights into understanding this disease. Western blot was employed to assess the protein levels of Bax and phospho-tau as well as phospho-JAK2; MTT assay was performed to decipher cell viability. Treatment of IL-5 decreased Aβ25-35-induced tau phosphorylation and apoptosis, effects blunted by JAK2 inhibition. IL-5 prevents Aβ25-35-evoked tau protein hyperphosphorylation and apoptosis through JAK2 signaling (AU)


No disponible


Assuntos
Animais , Ratos , Neurônios/metabolismo , Interleucina-5/metabolismo , Apoptose , Peptídeos beta-Amiloides/metabolismo , Subunidade alfa de Receptor de Interleucina-5/agonistas , Proteínas tau/metabolismo , Processamento de Proteína Pós-Traducional , Doença de Alzheimer , Sulfonamidas/farmacologia , Sobrevivência Celular , Ativação Enzimática , Janus Quinase 2 , Proteínas do Tecido Nervoso , Células PC12 , Pirrolidinas/farmacologia , Interferência de RNA , Inibidores de Proteínas Quinases/farmacologia
13.
J. physiol. biochem ; 73(1): 121-131, feb. 2017. tab, graf
Artigo em Inglês | IBECS | ID: ibc-168399

RESUMO

Methylglyoxal (MG) can react with amino acids of proteins to induce protein glycation and consequently the formation of advanced glycation end-products (AGEs). Previous studies reported that ferulic acid (FA) prevented glucose-, fructose-, and ribose-induced protein glycation. In this study, FA (0.1-1 mM) inhibited MG-induced protein glycation and oxidative protein damage in bovine serum albumin (BSA). Furthermore, FA (0.0125-0.2 mM) protected against lysine/MG-mediated oxidative DNA damage, thereby inhibiting superoxide anion and hydroxyl radical generation during lysine and MG reaction. In addition, FA did not have the ability to trap MG. Finally, FA (0.1 mM) pretreatment attenuated MG-induced decrease in cell viability and prevented MG-induced cell apoptosis in pancreatic β-cells. The results suggest that FA is capable of protecting β-cells from MG-induced cell damage during diabetes (AU)


No disponible


Assuntos
Animais , Ratos , Apoptose , Ácidos Cumáricos/farmacologia , Células Secretoras de Insulina , Dano ao DNA , Depuradores de Radicais Livres/farmacologia , Processamento de Proteína Pós-Traducional , Biomarcadores/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular , Produtos Finais de Glicação Avançada , Concentração Osmolar , Estresse Oxidativo , Substâncias Reativas com Ácido Tiobarbitúrico , Carbonilação Proteica
14.
Rev. iberoam. micol ; 32(3): 197-199, jul.-sept. 2015. tab
Artigo em Inglês | IBECS | ID: ibc-142081

RESUMO

Background. Both Cryptococcus neoformans and Cryptococcus gattii have been isolated from a variety of environmental sources in Colombia. Aim. To determine the viability of C. neoformans/C. gattii isolates in stored soil samples, filtrates and bird droppings from which these yeasts were previously recovered. Methods. A total of 964 samples collected between 2003 and 2009, and kept at room temperature were processed. From them, 653 samples were from trees decaying wood, 274 from soil filtrates and 37 from bird droppings. When C. neoformans or C. gattii were recovered, the molecular type of each isolate was established by PCR fingerprinting using the single primer (GTG)5. Results. Among the processed samples, 161 isolates were recovered. From those, 81 (50.3%) corresponded to C. gattii recovered from decaying wood of Eucalyptus spp., Corymbia ficifolia, Terminalia catappa and Ficus spp. trees, and 80 (49.7%) corresponded to C. neoformans recovered from Ficus spp. and eucalyptus trees, as well as from bird droppings. The most prevalent molecular type among the C. gattii and C. neoformans isolates was VGII and VNI, respectively. Conclusions . The re-isolation of C. neoformans/C. gattii from 10-year stored samples suggests that these yeasts are able to keep viable in naturally colonized samples (AU)


Antecedentes. Cryptococcus neoformans y Cryptococcus gattii han sido aislados de diversas fuentes ambientales en Colombia. Objetivos. Determinar la viabilidad de C. neoformans/C. gattii en muestras de suelo, filtrados y excrementos de aves en las que se habían aislado las levaduras previamente. Métodos. Se procesaron 964 muestras recogidas entre los años 2003 y 2009, y almacenadas a temperatura ambiente. De estas, 653 muestras provenían de detritos, 274 de filtrados de suelo y 37 de excrementos de aves. Una vez recuperados C. neoformans y C. gattii, se determinó el patrón molecular mediante la técnica de PCR huella digital con el iniciador (GTG)5. Resultados. Entre las muestras procesadas, se recuperaron 161 aislamientos. De estos, 81 (50,3%) fueron C. gattii recuperados de detritos de Eucalyptus spp., Corymbia ficifolia, Terminalia catappa y Ficus spp., y 80 (49,7%) eran C. neoformans recuperados de Ficus spp. y eucaliptos, así como de excrementos de aves. El patrón molecular más prevalente entre C. gattii y C. neoformans fue VGII y VNI, respectivamente. Conclusiones. El reaislamiento de C. neoformans/C. gattii de muestras almacenadas durante 10 años evidencia que estas levaduras son capaces de mantenerse viables en muestras colonizadas naturalmente (AU)


Assuntos
Cryptococcus neoformans/crescimento & desenvolvimento , Cryptococcus gattii/crescimento & desenvolvimento , Criptococose/microbiologia , Sobrevivência Celular , Viabilidade Microbiana , Leveduras/crescimento & desenvolvimento
15.
Arch. bronconeumol. (Ed. impr.) ; 51(7): 328-337, jul. 2015. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-138229

RESUMO

Introducción: El enfisema se ha asociado a una disminución de la expresión de VEGF y VEGFR-2 y a la presencia de un número elevado de células alveolares apoptósicas. El factor de crecimiento queratinocítico estimula la síntesis de VEGF, lo cual proporciona, a su vez, un mantenimiento de la estructura pulmonar normal a través de la vía de Akt. En este estudio hemos investigado el posible papel del rHuKGF en la mejora de la falta de regulación de la vía de supervivencia celular mediada por Akt en ratones enfisematosos. Métodos: Se establecieron 3 grupos experimentales: grupos de enfisema, tratamiento y control. Los pulmones de los ratones se trataron terapéuticamente en 3 ocasiones mediante la instilación orofaríngea de 10 mg de rHuKGF/kg de peso corporal tras la inducción del enfisema mediante elastasa pancreática porcina. Posteriormente, se obtuvo tejido pulmonar de los ratones para la realización de exámenes de histopatología y biología molecular. Resultados y discusión: Las microfotografías de histopatología y el análisis del índice de destrucción han mostrado que el agrandamiento del espacio aéreo inducido por la elastasa y la pérdida de alvéolos se recuperaron en el grupo de tratamiento. El rHuKGF estimula la producción de VEGF, que a su vez induce la vía de supervivencia celular mediada por Akt en los pulmones enfisematosos. Se produjo un aumento significativo de la expresión de mRNA de VEGF, VEGFR, PI3K y Akt, mientras que hubo una disminución notable de Pten, caspasa-9 y Bad en el grupo de tratamiento en comparación con el grupo de enfisema, y los resultados fueron comparables a los del grupo de control. Además, la expresión de VEGF a nivel proteico concordaba con la observada a nivel de mRNA. Conclusión: Los suplementos terapéuticos de rHuKGF mejoran la mala regulación de la vía de Akt en el trastorno del enfisema, dando lugar a una supervivencia celular alveolar a través de una activación de la vía de la supervivencia celular dependiente de VEGF endógena. Así pues, el rHuKGF podría ser un posible fármaco para el tratamiento del enfisema


Introduction: Emphysema has been associated with decreased VEGF andVEGFR-2 expression and the presence of high numbers of apoptotic alveolar cells. Keratinocyte growth factor stimulates VEGF synthesis which in turn confers normal lung structure maintenance via the Akt pathway. In this study the potential role of rHuKGF in the improvement of deregulated Akt mediated cell survival pathway in emphysematous mice was investigated Methods: Three experimental groups, i.e., emphysema, treatment and control groups, were prepared. Lungs of mice were treated on 3 occasions by oropharyngeal instillation of 10 mg rHuKGF per kg body weight after induction of emphysema with porcine pancreatic elastase. Subsequently, lung tissues from mice were collected for histopathology and molecular biology studies. Results and discussion: Histopathology photomicrographs and destructive index analysis have shown that elastase-induced airspace enlargement and loss of alveoli recovered in the treatment group. rHuKGF stimulates VEGF production which in turn induces the Akt mediated cell survival pathway in emphysematous lungs. mRNA expression of VEGF, VEGFR, PI3K and Akt was significantly increased while Pten, Caspase-9 and Bad was notably decreased in treatment group when compared with emphysema group, being comparable with the control group. Moreover, VEGF protein expression was in accordance with that found for mRNA. Conclusion: Therapeutic rHuKGF supplementation improves the deregulated Akt pathway in emphysema, resulting in alveolar cell survival through activation of the endogenous VEGF-dependent cell survival pathway. Hence rHuKGF may prove to be a potential drug in the treatment of emphysema


Assuntos
Animais , Camundongos , Peptídeos e Proteínas de Sinalização Intercelular/farmacocinética , Proteína Oncogênica v-akt , Enfisema/fisiopatologia , Queratinócitos , Sobrevivência Celular , Substâncias Protetoras/farmacocinética , Modelos Animais de Doenças
16.
Med. oral patol. oral cir. bucal (Internet) ; 20(3): e273-e277, mayo 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-139041

RESUMO

BACKGROUND: This study is conducted mainly to evaluate the changes in quality and quantity of oral epithelial cells during the course of IMRT.MATERIAL AND METHODS:30 Patients undergoing chemo-radiotherapy were followed through course of treatment. They were compared with a group of age- and sex-matched healthy individuals. The procedure involved WHO clinical scoring, collection of oral washings and preparation of buccal smears from both study group and control group. The changes occurred were recorded as a way of assessing the severity of oral mucositis. RESULTS: revealed a significant occurrence of oral mucositis in almost all patients during weekly follow up. There was a significant increase in percentage of viable buccal epithelial cells in study group when compared to normal controls (P<0.005) during and at the end of chemo-radiotherapy. CONCLUSIONS: quantification of oral mucositis can be done at cellular level by determining the oral mucosal cell viability and their maturation during IMRT (AU)


Assuntos
Humanos , Quimiorradioterapia/efeitos adversos , Estomatite/epidemiologia , Neoplasias de Cabeça e Pescoço/complicações , Sobrevivência Celular , Sobrevivência Celular/efeitos da radiação , Radioterapia de Intensidade Modulada/efeitos adversos , Técnicas In Vitro/métodos
17.
Clin. transl. oncol. (Print) ; 17(4): 314-321, abr. 2015. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-134251

RESUMO

Objectives: A variety of inflammatory cytokines have been demonstrated to participate in tumorigenesis and progression. Secretory leukocyte protease inhibitor (SLPI) has been demonstrated to show a broad-spectrum of anti-inflammatory effects. This study investigates the expression of SLPI in human pancreatic cancer tissues and cells as well as its biological effects in human pancreatic cancer cells. Methods: Reverse transcription-polymerase chain reaction, immunohistochemistry, and Western blot were used to detect SLPI mRNA and protein levels in human pancreatic cancer tissues, adjacent tissues, and pancreatic cancer Bxpc-3 and Panc-1 cells. Knockout of SLPI expression was established by recombinant viral vector expressing short hairpin RNA (shRNA) targeting SLPI. Cell viability was analyzed by MTT assay. Cell apoptosis was detected by Hochest33258 staining and flow cytometry assay. Results: Higher SLPI expression was observed in pancreatic tissues, Bxpc-3 cells, and Panc-1 cells compared to the peritumoral tissues (p < 0.01). SLPI expression in Bxpc-3 and Panc-1 cells was effectively silenced by shRNA (p < 0.001). Silencing of SLPI expression significantly reduced cell viability, inhibited cell proliferation, and induced cell apoptosis (p < 0.001). Conclusions: Abnormal over-expression of SLPI in pancreatic cancer cells may be associated with the development of disease through its roles in promoting cancer cell survival and proliferation as well as anti-apoptosis. SLPI can be used as a target for developing targeted therapy of pancreatic cancer (AU)


No disponible


Assuntos
Humanos , Inibidor Secretado de Peptidases Leucocitárias/farmacocinética , Neoplasias Pancreáticas/patologia , Ilhotas Pancreáticas/patologia , Proteínas Reguladoras de Apoptose/farmacocinética , Proliferação de Células , Sobrevivência Celular
18.
An. R. Acad. Farm ; 80(4): 720-734, oct.-dic. 2014. ilus
Artigo em Inglês | IBECS | ID: ibc-132485

RESUMO

Apoptosis is an important cellular physiological function to continually balance the cell proliferation with cell death to maintain the healthy internal milieu. This study assessed the ability of Curcumin to inhibit 3T3-L1 adipocytes viability and its effects on apoptosis to identify a potential molecular approach for preventing adipocytes growth. To optimize the growth inhibitory concentration 3T3-L1 adipocytes treated with different concentrations of Curcumin (5, 10, 20, 40, 60, and 80 μM) for 24 hours, and were analyzed by MTT and found 40 μM as an optimal dose. Cell cycle was analysed by FACS using the optimal dose for different periods (4-24h). Western blot was carried out using antibodies to detect protein expression and Phosphorylation of Adiponectin, AMPK, CREB, PKA catalytic subunit, and Akt. PKA relative activity and ROS generation was also assessed by DCFHDA fluorescence. The findings of the study revealed that Curcumin has successfully arrested 3T3L1 adipocytes cycle and exerted apoptotic action on the cells in a concentration- time dependent manner; increased Adiponectin expression; activated phosphorylated AMPK (p-AMPK) and inhibited PKA activation. Curcumin prevented the production of ROS generation. Results indicate that Curcumin exerts a very potent apoptotic action on 3T3L1 adipocytes and modulates adiponectin expression and AMPK and PKA signalling. These findings lead us to further investigate and support the potential use of Curcumin therapy in obesity resistance


La apoptosis es una función fisiológica celular importante para equilibrar de manera continua la proliferación y la muerte celulares con el objeto de mantener el entorno interno sano. Este estudio se determina la capacidad de la curcumina de inhibir la viabilidad de los adipocitos 3T3-L1 y sus efectos sobre la apoptosis para identificar un posible acercamiento molecular para prevenir el crecimiento de los adipocitos. Para optimizar la concentración inhibitoria del crecimiento, los adipocitos 3T3-L1 han sido tratados con diversas concentraciones de curcumina (5, el μM 10, 20, 40, 60, y 80) durante 24 horas y analizados por MTT, encontrando el μM 40 como dosis óptima. El ciclo de la célula ha sido analizado por FACS usando la dosis óptima en diversos períodos (4-24h). El Western blot se realizó usando los anticuerpos para detectar la proteína y la fosforilación de la adiponectina, de la subunidad catalítica de AMPK, de CREB, de PKA, y de Akt. La actividad relativa de PKA y la generación del ROS también fueron determinadas por fluorescencia de DCFHDA. Los resultados del estudio revelaron que la curcumina ha detenido con éxito el ciclo de los adipocitos 3T3L1 y que la acción apoptótica ejercida en las células en una concentración mide el tiempo de manera dependiente; expresión creciente de adiponectina; AMPK fosforilado activado (p-AMPK) y activación inhibida de PKA. La curcumina previno la producción de la generación del ROS. Los resultados indican que la curcumina ejerce una acción apoptótica muy potente en los adipocitos 3T3L1 y modula la expresión de la adiponectina indicando AMPK y PKA. Estos resultados nos conducen a ir más lejos en la investigación y el uso potencial de la terapia de la curcumina para combatir la obesidad


Assuntos
Humanos , Adipócitos , Curcumina/farmacocinética , Sobrevivência Celular , Obesidade/fisiopatologia , Adiponectina , Obesidade/tratamento farmacológico , Fármacos Antiobesidade/farmacologia
19.
Int. microbiol ; 17(3): 131-139, sept. 2014. ilus
Artigo em Inglês | IBECS | ID: ibc-132087

RESUMO

In this study, we analyzed the metabolite features of the yeasts Saccharomyces cerevisiae, Naumovia castellii, and Saccharomyces mikatae. The three species are closely related genetically but differ in their tolerance of desiccation stress. Specifically, we determined whether certain metabolites correlated with cell viability after stress imposition. The metabolomic profiles of these strains were compared before cell desiccation and after cell rehydration. In S. mikatae, the presence of lysine or glutamine during rehydration led to a 20% increase in survival whereas during dehydration the levels of both amino acids in this yeast were drastically reduced (AU)


No disponible


Assuntos
Humanos , Metabolômica/métodos , Leveduras/metabolismo , Desidratação/microbiologia , Saccharomyces/metabolismo , Saccharomyces cerevisiae/metabolismo , Sobrevivência Celular/imunologia
20.
Nutr. hosp ; 29(2): 388-392, 2014. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-120600

RESUMO

Background: Silibinin a flavonoid from milk thistle(Silybum marianum) exhibit a variety of pharmacological actions, including anti-proliferative and apoptotic activities against various types of cancers in intact animals and cancer cell lines. In the present study, the effect of silibininon human colon cancer HT-29 cells was studied. Method: Incubations of cells with different silibinin concentrations (0.783-1,600 ug/ml) for 24, 48 or 72 h showed a progressive decline in cell viability. Results: Loss of cell viability was time dependent and optimum inhibition of cell growth (78%) was observed at72 h. Under inverted microscope, the dead cells were seenas cell aggregates. IC50 (silibinin concentration killing50% cells) values were 180, 110 and 40ug/ml at 24, 48 and72 h respectively. Conclusion: These findings re-enforce the anticancer potential of silibinin, as reported earlier for various other cancer cell lines (Ramasamy and Agarwal (2008), Cancer Letters, 269: 352-62) (AU)


Antecedentes: Silibinina un flavonoide a partir de laleche de cardo mariano (Silybum marianum) exhiben una variedad de acciones farmacológicas, incluyendo actividad esantiproliferativos y apoptóticos contra varios tipos de cánceres en animales intactos y líneas celulares de cáncer. En el presente estudio, se estudió el efecto de silibinina en células humanas de cáncer de colon HT-29.Método: Las incubaciones de las células con diferentes concentraciones silibinin (0,783-1.600 ug/ml) para 24, 48o 72 horas mostró un descenso progresivo de la viabilidad celular. Resultados: La pérdida de la viabilidad celular fue de tiempo de inhibición dependiente y óptima de crecimiento de las células (78%) se observó a las 72 horas. Bajo microscopio invertido, las células muertas fueron vistos como los agregados de células. IC50 (concentración de silibinina matar a las células 50%) los valores fueron 180,110 y 40 ug/ml a las 24, 48 y 72 horas, respectivamente. Conclusión: Estos resultados volver a hacer cumplir la potenciales contra el cáncer de silibinina, como se informó anteriormente para varias otras líneas celulares de cáncer (Ramasamy y Agarwal (2008), Cancer Letters,269: 352-62) (AU)


Assuntos
Humanos , Neoplasias do Colo/tratamento farmacológico , Tumor Adenomatoide/tratamento farmacológico , Células HT29/patologia , Flavonoides/farmacocinética , Cardo-Mariano , Proliferação de Células , Sobrevivência Celular , Extratos Vegetais/farmacocinética
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