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1.
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1102964

RESUMO

Introducción: las perforaciones septales y su corrección quirúrgica constituyen un reto para los otorrinolaringólogos. En la literatura se describen diferentes técnicas para el cierre de las perforaciones septales; sin embargo, sus resultados en términos de efectividad son muy variables y con pocos pacientes. Desde hace 8 años se viene realizando la técnica de cierre de perforación septal con injertos de cartílago en el servicio de otorrinolaringología del Hospital de San José, y se ha observado una respuesta clínica exitosa. El objetivo del presente estudio fue describir los resultados posoperatorios de los pacientes manejados con la técnica de cierre quirúrgico de perforación septal con injertos de cartílago, en términos de aparición de complicaciones y frecuencia de perforación septal residual. Materiales y métodos: mediante un estudio de cohorte descriptiva se presentan los pacientes manejados con la técnica de cierre quirúrgico de perforación septal con injertos de cartílago de banco o cartílago autólogo. Se incluyen pacientes a partir enero de 2014 a junio 2018. De la historia clínica se extrajeron los datos demográficos, clínicos, complicaciones y presentación de perforación septal residual. Resultados: la tasa de éxito de cierre de perforación septal fue de 78,3 %; siendo las etiologías más frecuentes antecedente de cirugía e idiopática. La complicación más común fue epistaxis en el 26 % de los pacientes, seguida de dolor en el 21,7 % en el posoperatorio mediato, el cual mejoró en los controles posteriores. Conclusión: los resultados con la técnica de cierre de perforación septal con injerto de banco fueron satisfactorios en esta población.


Introduction: septal perforations and surgical correction are a challenge for ENT specialists. Several techniques for closing septal perforations are described in the literature; however, its results in terms of effectiveness are variable and with small sample sizes. The technique of closure of septal perforation with cartilage grafts has been performed for 8 years in the ENT department of Hospital de San José with a successful clinical response. Aims: to describe the postoperative results of patients managed with the technique of surgical closure of septal perforation with cartilage grafts, in terms of complications and frequency of residual septal perforation. Design: descriptive cohort study. Methods: a cohort of patients managed with the surgical closure technique of septal perforation with grafts of bank cartilage or autologous cartilage are described. Patients were included from January 2014 to June 2018. Demographic, clinical data, complications and presentation of residual septal perforation were extracted from the clinical history. Results: The success rate of septal perforation closure was 78.3 %; being the most frequent etiologies antecedent of surgery and idiopathic. The most common complication was epistaxis in 26 % of patients, followed by pain in 21.7 % in the postoperative period, which improved in subsequent controls. Conclusion: the results with the technique of closure of septal perforation with bank grafting were satisfactory in this population.


Assuntos
Humanos , Perfuração do Septo Nasal , Cartilagem , Deformidades Adquiridas Nasais , Procedimentos Cirúrgicos Reconstrutivos
2.
Rev. bras. ortop ; 54(3): 316-321, May-June 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1013716

RESUMO

Abstract Objective Focal osteochondral lesions of the knee are found in two thirds of patients undergoing arthroscopy; their treatment, when isolated and especially in young individuals, remains a debating topic. The present study analyzes the results obtained by the application of the mosaicplasty technique on the treatment of isolated knee femoral condyle osteochondral lesions. Methods Retrospective study of patients submitted tomosaicplasty and to subjective analyseswith pre- and postsurgery International KneeDocumentation Committee (IKDC) scores. Results A total of 13 cases with an average age of 34 years old, with male patients (n = 4; 31%) with an average age of 23 years old (range: 17-31 years old), and female patients (n = 9; 69%) with an average age of 39 years old (range: 16-56 years old); medial versus lateral femoral (n = 11; 85% versus n = 2; 15%); the average size of the lesion was 1.8 cm2 (range: 0.6-4 cm2); average follow-up time: 5.045 ± 3.47 years (range: 1.15-11.01 years). The average preoperative IKDC score was of 31.63 points (± 20.24), the average postoperative IKDC score was of 74.18 points (± 20.26). The difference between the post- and preoperative IKDC scores was of 42.55 (± 21.05) points, being theminimal score increase of 8.1 points andthemaximumscore increaseof82.8 points.Astatistically significant difference (p < 0.001) was found between the IKDC scores before and after the surgery. A statistically significant relation (p = 0.038) was found between the IKDC score increase (the difference between the postoperative and the preoperative scores) and the dimension of the lesion. Conclusions Mosaicplasty with osteochondral autograft transfer, when adequately used, can produce excellent results with great durability and functional impact, low morbidity rates and costs. Expansion of the indication criteria shows promising midterm and long-term results.


Resumo Objetivo Lesões osteocondrais focais do joelho são encontradas em dois terços dos pacientes submetidos a artroscopia; seu tratamento, quando isoladas e, principalmente, em indivíduos jovens, ainda é debatido. O presente estudo analisa os resultados obtidos com a aplicação da técnica de mosaicoplastia no tratamento de lesões osteocondrais isoladas do côndilo femoral do joelho. Métodos Estudo retrospectivo de pacientes submetidos à mosaicoplastia e análise subjetiva com pontuações do International Knee Documentation Committee (IKDC, na sigla em inglês) antes e após a cirurgia. Resultados Um total de 13 casos, com média de idade de 34 anos; pacientes do sexo masculino (n = 4; 31%) apresentaram média de idade de 23 anos (17-31 anos), e pacientes do sexo feminino (n = 9; 69%) apresentaram média de 39 anos; (16-56 anos); femoral medial ou lateral (n = 11, 85% versus n = 2, 15%, respectivamente); o tamanho médio da lesão foi de 1,8 cm2 (0,6-4 cm); o tempo médio de acompanhamento foi de 5,045 ± 3,47 anos (1,15-11,01 anos). A pontuação IKDC média préoperatória foi 31,63 pontos ( ± 20,24), e a pós-operatória foi 74,18 pontos ( ± 20,26). A diferença entre as pontuações IKDC obtidas depois e antes da cirurgia foi de 42,55 ( ± 21,05) pontos, com o aumento mínimo de 8,1 pontos e o aumento máximo de 82,8 pontos. Uma diferença estatística significativa (p < 0,001) foi encontrada entre a pontuação IKDC antes e após a cirurgia. Uma relação estatisticamente significativa (p = 0,038) foi observada entre o aumento da pontuação IKDC (a diferença entre a pontuação pré- e pós-operatória) e as dimensões da lesão. Conclusões A mosaicoplastia com transferência de autoenxerto osteocondral, quando adequadamente usada, pode produzir resultados excelentes com grande durabilidade e impacto funcional, baixas taxas de morbidade e baixos custos. A expansão dos critérios de indicação mostra resultados promissores no médio e longo prazo.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Osteocondrite/cirurgia , Osteocondrite/diagnóstico , Artroscopia , Cartilagem , Cartilagem Articular , Articulação do Joelho
3.
Int. arch. otorhinolaryngol. (Impr.) ; 22(4): 374-377, Oct.-Dec. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-975610

RESUMO

Abstract Introduction Different types of autologous graft materials are used for myringoplasty, with the temporalis fascia and cartilage being the most frequently used tissues. Periosteal tissue has been used for a long time in our department, and many advantages support its use in myringoplasty. To the best of our knowledge, this issue is scarcely discussed in the previously published literature. Objective To present our experience with periosteal graft myringoplasty, describing the technique and the anatomical and functional outcomes. Methods A prospective clinical study involving 88 patients (72 females and 16 males) with a mean age 26.9 years. The patients underwent myringoplasty using the mastoid cortex periosteum; they were all operated using the postauricular approach, and the graft was applied using the underlay technique. The patients performed pre- and postoperative pure tone audiometry for tested frequencies (0.5 kHz, 1 kHz, 2 kHz, and 4 kHz). All patients were followed-up for at least 12 months after the operation. Results The anatomical success rate among all patients was of 93%, which is comparable to the rate of success in procedures using other usual grafting materials. In addition, there was a highly significant postoperative improvement in pure tone audiometry results as compared with the preoperative ones (the main hearing gain was of ∼ 11 dB; p< 0.001). Conclusion The periosteal graft is easily harvested, easy to apply, with excellent anatomical and functional success.


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Periósteo/transplante , Miringoplastia , Audiometria de Tons Puros , Transplante Autólogo , Membrana Timpânica/cirurgia , Cartilagem/transplante , Estudos Prospectivos , Fáscia/transplante
4.
Med. UIS ; 31(3): 47-56, sep.-dic. 2018. graf
Artigo em Espanhol | LILACS | ID: biblio-1002519

RESUMO

Resumen Introducción: el cartílago es un tejido conectivo especializado ampliamente estudiado por sus componentes mecánicos y su aporte para el funcionamiento articular. El entendimiento de su rol requiere necesariamente del abordaje del comportamiento biomecánico. Objetivo: realizar una revisión de literatura acerca de la biomecánica del cartílago articular y sus respuestas a las fuerzas aplicadas. Materiales y Métodos: se realizó una búsqueda bibliográfica en las bases de datos Pubmed, Scielo, Science Direct y Google académico de artículos publicados entre los años 1998 y 2017, con los términos: "Cartilage Biomechanic", "Cartilage Fisiology", y "Cartilage Histology". Se encontraron 55 artículos, 44 en idioma inglés y 11 en idioma español, los cuales contenían información relevante a cerca de la biomecánica del cartílago articular. Resultados: en este artículo se resume un conjunto de conceptos derivados de estudios experimentales y otras revisiones de tema, abordando actualizaciones en cuanto a la histología, la fisiología y las diferentes respuestas mecánicas ante distintos estímulos como lo son la anisotropía, la viscoelasticidad la histéresis, fluencia, entre otros. Conclusiones: el cartílago articular es un tejido conectivo trifásico que permite el soporte y transmisión de cargas gracias a la mecanotransducción. El abordaje y comprensión de la biomecánica de los tejidos se hace necesaria para la prescripción del ejercicio en condiciones aparentemente normales y patológicas. MÉD.UIS. 2018;31(3):47-56.


Abstract Background: cartilage is a specialized connective tissue widely studied for its mechanical components and its contribution to joint functioning. The understanding of cartilage role necessarily requires an approach of biomechanical behavior. Objective: to perform a literature review about the biomechanics of the articular cartilage and its responses to applied forces. Materials and Methods: a bibliographic search was conducted in Pubmed, Scielo, Science Direct and Google academic databases, of articles published between 1998 and 2017, with the terms: "Cartilage Biomechanic", "Cartilage Physiology", and "Cartilage Histology". 55 articles were found, 44 in English and 11 in Spanish, which contained relevant information about the biomechanics of articular cartilage. Results: this article summarizes a set of concepts derived from experimental studies and other reviews of the topic, addressing updates regarding histology, physiology and different mechanical responses to different stimuli such as anisotropy, viscoelasticity, hysteresis and fluency. Conclusions: the articular cartilage is a three-phase connective tissue that allows the support and transmission of loads thanks to the mechanotransduction. The approach and understanding of the biomechanics of the tissues is necessary for the prescription of exercise in apparently normal and pathological conditions. MÉD.UIS. 2018;31(3):47-56.


Assuntos
Humanos , Fenômenos Biomecânicos , Cartilagem , Condrócitos , Módulo de Elasticidade , Histologia
5.
Rev. bras. ortop ; 53(1): 45-52, Jan.-Feb. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-899238

RESUMO

ABSTRACT Objective: To evaluate the ability of the eluate from fibrin-rich plasma (FRP) membrane to induce proliferation and differentiation of isolated human adipose-derived stem cells (ASCs) into chondrocytes. Method: FRP membranes were obtained by centrifugation of peripheral blood from two healthy donors, cut, and maintained in culture plate wells for 48 h to prepare the fibrin eluate. The SCATh were isolated from adipose tissue by collagenase digestion solution, and expanded in vitro. Cells were expanded and treated with DMEM-F12 culture, a commercial media for chondrogenic differentiation, and eluate from FRP membrane for three days, and labeled with BrdU for quantitative assessment of cell proliferation using the High-Content Operetta® imaging system. For the chondrogenic differentiation assay, the SCATh were grown in micromass for 21 days and stained with toluidine blue and aggrecan for qualitative evaluation by light microscopy. The statistical analysis was performed using ANOVA and Tukey's test. Results: There was a greater proliferation of cells treated with the eluate from FRP membrane compared to the other two treatments, where the ANOVA test showed significance (p < 0.001). The differentiation into chondrocytes was visualized by the presence of mucopolysaccharide in the matrix of the cells marked in blue toluidine and aggrecan. Conclusions: Treatment with eluate from FRP membrane stimulated cell proliferation and induced differentiation of the stem cells into chondrocytes, suggesting a potential application of FRP membranes in hyaline cartilage regeneration therapies.


RESUMO Objetivo: Avaliar a capacidade do eluato proveniente da membrana de plasma rico em fibrina (PRF) de induzir proliferação e diferenciação das células-tronco humanas isoladas de tecido adiposo (CTDAh) em condrócitos. Método: As membranas de PRF foram obtidas por centrifugação de sangue periférico de dois indivíduos saudáveis, cortadas, colocadas em poços de placa de cultivo por 48 h para obtenção do eluato de fibrina. As CTDAh foram isoladas do tecido adiposo por digestão com solução de colagenase e expandidas in vitro. As células foram expandidas e tratadas com meio de cultivo DMEM-F12, meio comercial para diferenciação condrocítica, e eluato de fibrina durante três dias e marcadas com BrdU para avaliação quantitativa da proliferação celular com o uso do sistema de imagens High-Content Operetta®. Para o ensaio de diferenciação condrogênica, as CTDAh foram cultivadas em micromassa por 21 dias e coradas com azul de toluidina e agrecana para avaliação qualitativa em microscópio óptico. As avaliações estatísticas foram feitas por meio dos testes Anova e Tukey. Resultados: Houve uma maior proliferação das células tratadas com o eluato de fibrina comparativamente com os outros dois tratamentos, nos quais o teste Anova apontou significância (p < 0,001). A diferenciação em condrócitos foi visualizada pela presença de mucopolissacarídeos na matriz das células tratadas com meio de diferenciação ou eluato e marcação positiva para agrecana. Conclusões: O tratamento com o eluato da membrana de fibrina estimulou a proliferação celular e induziu a diferenciação das células-tronco em condrócitos, o que sugere uma potencial aplicação da membrana de PRF nas terapias de regeneração de cartilagem hialina.


Assuntos
Humanos , Cartilagem , Membranas , Plasma Rico em Plaquetas , Regeneração
6.
J. oral res. (Impresa) ; 6(11): 291-298, nov. 30, 2017. tab
Artigo em Inglês | LILACS | ID: biblio-1118598

RESUMO

The aim of this study is to establish the position of the inferior alveolar nerve in relation to the Meckel's cartilage, the anlage of the mandibular body and primordia of the teeth, and also to trace the change in nerve trunk structure in the human prenatal ontogenesis. serial sections (20µm) from thirty-two 6-12 weeks-old entire human embryos and serial sections (10µm) of six mandibles of 13-20 weeks-old human fetuses without developmental abnormalities were studied. histological sections were impregnated with silver nitrate according to Bilshovsky-Buke and stained with hematoxylin and eosin. during embryonic development, the number of branches of the inferior alveolar nerve increases and its fascicular structure changes. in conclusion, the architecture of intraosseous canals in the body of the mandible, as well as the location of the foramina, is predetermined by the course and pattern of the vessel/nerve branching in the mandibular arch, even before the formation of bony trabeculae. particularly, the formation of the incisive canal of the mandible can be explained by the presence of the incisive nerve as the extension of the inferior alveolar nerve. It has also been established that Meckel's cartilage does not participate in mandibular canal morphogenesis.


Assuntos
Humanos , Raiz Dentária/anatomia & histologia , Nervo Trigêmeo/anatomia & histologia , Nervo Mandibular/anatomia & histologia , Cartilagem/anatomia & histologia , República de Belarus , Desenvolvimento Embrionário e Fetal , Nervo Mandibular/crescimento & desenvolvimento
7.
Actual. osteol ; 13(1): 58-66, Ene - Abr. 2017. ilus
Artigo em Inglês | LILACS | ID: biblio-1118913

RESUMO

Connexins (Cxs) are a family of transmembrane proteins that form gap junctions and hemi-channels, which mediate cell-cell communication between neighboring cells and the respective extracellular milieu in different tissues. Most tissues and cell types throughout the body express one or more Cx proteins, highlighting its importance in regulating cell growth, differentiation, adhesion, migration, cell death and others. Moreover, Cx can propagate intracellular signals through its C-terminus domain, and thus function beyond a mere channel. Cx43 is the most highly expressed and most well studied Cx in bone and musculoskeletal tissues, although Cx40, Cx45, Cx46 and more recently, the Cx37 have been described in bone tissue, along with Cx26, Cx32 and Cx39 in other musculoskeletal tissues. Here, we discuss the basic structure of gap junctions and the role of the Cxs in musculoskeletal tissue, with special focus on Cx37. (AU)


Las conexinas (Cxs) son una familia de proteínas transmembrana que forman uniones en hendidura y hemicanales encargados de mediar la comunicación entre células vecinas y el respectivo medio extracelular en diferentes tejidos. La mayoría de los tejidos y células expresan una o más proteínas conexina, jugando un papel importante en la regulación de la proliferación celular, diferenciación, adhesión, migración y muerte celular, entre otras funciones. Además de actuar como un canal, las conexinas pueden propagar señales intracelulares a través del dominio C-terminal. La Cx43 es la conexina mas expresada y mejor estudiada en el tejido óseo y el músculo, aunque las Cx40, Cx45, Cx46, y mas recientemente Cx37, son también detectadas en el hueso. A su vez la expresión de la Cx26, Cx32 y Cx39 ha sido observada en otros tejidos músculoesqueléticos. En este manuscrito describimos la estructura básica de las uniones tipo gap y el papel que las Cxs, y en especial la Cx37, tienen en tejidos músculo-esqueléticos. (AU)


Assuntos
Humanos , Osso e Ossos/metabolismo , Reabsorção Óssea/prevenção & controle , Conexinas/fisiologia , Osteoblastos/metabolismo , Osteócitos/metabolismo , Tendões/metabolismo , Transdução de Sinais/fisiologia , Cartilagem/metabolismo , Comunicação Celular/fisiologia , Fenômenos Fisiológicos Celulares , Junções Comunicantes/efeitos dos fármacos , Junções Comunicantes/fisiologia , Conexina 43/fisiologia , Músculo Esquelético/metabolismo , Conservadores da Densidade Óssea/uso terapêutico , Ligamentos/metabolismo , Antiarrítmicos/efeitos adversos
8.
An. bras. dermatol ; 91(5,supl.1): 140-143, Sept.-Oct. 2016. graf
Artigo em Inglês | LILACS | ID: biblio-837938

RESUMO

Abstract The paramedian forehead flap is a great option for restoration of complex nasal defects. For full-thickness defects, it may be used alone or in combination with other methods. We present a patient with a basal cell carcinoma on the distal nose treated by Mohs micrographic surgery, and a resulting full-thickness defect repaired with paramedian forehead flap combined with a hinge flap. For optimal results with the paramedian forehead flap, adequate surgical planning, patient orientation and meticulous surgical technique are imperative.


Assuntos
Humanos , Masculino , Idoso , Neoplasias Cutâneas/cirurgia , Retalhos Cirúrgicos , Carcinoma Basocelular/cirurgia , Neoplasias Nasais/cirurgia , Cirurgia de Mohs/métodos , Fatores de Tempo , Cartilagem/cirurgia , Nariz/cirurgia , Reprodutibilidade dos Testes , Resultado do Tratamento , Testa
9.
Clinics ; 71(7): 404-411, tab, graf
Artigo em Inglês | LILACS | ID: lil-787438

RESUMO

OBJECTIVES: To evaluate the accuracy of magnetic resonance imaging measurements of cartilage tissue-mimicking phantoms and to determine a combination of magnetic resonance imaging parameters to optimize accuracy while minimizing scan time. METHOD: Edge dimensions from 4 rectangular agar phantoms ranging from 10.5 to 14.5 mm in length and 1.25 to 5.5 mm in width were independently measured by two readers using a steel ruler. Coronal T1 spin echo (T1 SE), fast spoiled gradient-recalled echo (FSPGR) and multiplanar gradient-recalled echo (GRE MPGR) sequences were used to obtain phantom images on a 1.5-T scanner. RESULTS: Inter- and intra-reader reliability were high for both direct measurements and for magnetic resonance imaging measurements of phantoms. Statistically significant differences were noted between the mean direct measurements and the mean magnetic resonance imaging measurements for phantom 1 when using a GRE MPGR sequence (512x512 pixels, 1.5-mm slice thickness, 5:49 min scan time), while borderline differences were noted for T1 SE sequences with the following parameters: 320x320 pixels, 1.5-mm slice thickness, 6:11 min scan time; 320x320 pixels, 4-mm slice thickness, 6:11 min scan time; and 512x512 pixels, 1.5-mm slice thickness, 9:48 min scan time. Borderline differences were also noted when using a FSPGR sequence with 512x512 pixels, a 1.5-mm slice thickness and a 3:36 min scan time. CONCLUSIONS: FSPGR sequences, regardless of the magnetic resonance imaging parameter combination used, provided accurate measurements. The GRE MPGR sequence using 512x512 pixels, a 1.5-mm slice thickness and a 5:49 min scan time and, to a lesser degree, all tested T1 SE sequences produced suboptimal accuracy when measuring the widest phantom.


Assuntos
Cartilagem/diagnóstico por imagem , Precisão da Medição Dimensional , Imagem por Ressonância Magnética/métodos , Imagens de Fantasmas , Variações Dependentes do Observador , Interpretação de Imagem Radiográfica Assistida por Computador , Valores de Referência , Reprodutibilidade dos Testes , Razão Sinal-Ruído , Fatores de Tempo
10.
Rev. bras. cir. plást ; 31(1): 66-73, jan.-mar. 2016. ilus, tab
Artigo em Inglês, Português | LILACS | ID: biblio-1503

RESUMO

INTRODUÇÃO: A proeminência dos pavilhões auriculares é a forma mais comum de deformidade desta estrutura, afetando em torno de 5% da população. A maioria dos trabalhos acerca da cirurgia de otoplastia apresenta avaliações subjetivas, não permitindo apreciar acuradamente os resultados no pós-operatório, bem como dificultando a comparação entre técnicas. Propõe-se o desenvolvimento e aplicação de protocolo específico para avaliação dos resultados. MÉTODO: Avaliação prospectiva pelo período de um ano de pacientes submetidos à otoplastia bilateral utilizando técnica baseada em modelagem cartilaginosa com suturas, utilizando medidas da distância hélice-mastoide em pontos padronizados. RESULTADOS: Foram operados 23 pacientes com idade média de 17,8 anos. A incidência de reoperação foi de 21,7% dos pacientes ou 10,7% das orelhas. Houve perda de cerca de 45% da correção obtida no ponto superior e 35% nos pontos médio e inferior nos pacientes não reoperados. CONCLUSÕES: O protocolo foi de fácil utilização e permitiu a avaliação objetiva tanto da deformidade no pré-operatório quanto dos resultados cirúrgicos. A técnica utilizada produziu resultados considerados adequados e comparáveis aos da literatura.


INTRODUCTION: Prominence is the most common deformity of the ear, affecting about 5% of the population. Most reports on otoplasty describe subjective evaluations, and do not provide accurate postoperative assessment or a comparison between techniques. We propose the development and implementation of a specific protocol to evaluate results. METHOD: A prospective evaluation for a period of one year in patients who underwent bilateral otoplasty was performed, using a technique based on modeling of the cartilage with sutures, and helix-to-mastoid distance measurements at standardized points. RESULTS: A total of 23 patients with an average age of 17.8 years underwent surgery. Reoperation was performed in 21.7% of the patients or 10.7% of the ears. Nearly 45% of the correction obtained at the upper point and 35% at the middle and lower points were lost in patients who did not undergo reoperation. CONCLUSIONS: The protocol was easily used and allowed objective evaluation of the preoperative deformity and surgical results. This technique produced results considered adequate and comparable to the literature.


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto , História do Século XXI , Suturas , Cartilagem , Antropometria , Protocolos Clínicos , Estudos Prospectivos , Estudos Retrospectivos , Procedimentos Cirúrgicos Reconstrutivos , Estudo de Avaliação , Orelha , Deformidades Adquiridas da Orelha , Orelha Externa , Suturas/normas , Cartilagem/cirurgia , Antropometria/instrumentação , Antropometria/métodos , Protocolos Clínicos/normas , Procedimentos Cirúrgicos Reconstrutivos/métodos , Orelha/cirurgia , Deformidades Adquiridas da Orelha/cirurgia , Deformidades Adquiridas da Orelha/patologia , Orelha Externa/cirurgia
11.
Acta cir. bras ; 31(2): 74-83, Feb. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-775567

RESUMO

PURPOSE: To investigated the effects of exposure to an 1800 MHz electromagnetic field (EMF) on bone development during the prenatal period in rats. METHODS: Pregnant rats in the experimental group were exposed to radiation for six, 12, and 24 hours daily for 20 days. No radiation was given to the pregnant rats in the control group. We distributed the newborn rats into four groups according to prenatal EMF exposure as follows: Group 1 was not exposed to EMF; groups 2, 3, and 4 were exposed to EMF for six, 12, and 24 hours a day, respectively. The rats were evaluated at the end of the 60th day following birth. RESULTS: Increasing the duration of EMF exposure during the prenatal period resulted in a significant reduction of resting cartilage levels and a significant increase in the number of apoptotic chondrocytes and myocytes. There was also a reduction in calcineurin activities in both bone and muscle tissues. We observed that the development of the femur, tibia, and ulna were negatively affected, especially with a daily EMF exposure of 24 hours. CONCLUSION: Bone and muscle tissue development was negatively affected due to prenatal exposure to 1800 MHz radiofrequency electromagnetic field.


Assuntos
Humanos , Animais , Masculino , Feminino , Recém-Nascido , Efeitos Tardios da Exposição Pré-Natal/patologia , Desenvolvimento Ósseo/efeitos da radiação , Calcineurina/metabolismo , Campos Eletromagnéticos/efeitos adversos , Fatores de Tempo , Gravidez , Cartilagem/patologia , Ratos Sprague-Dawley , Apoptose/efeitos da radiação , Condrócitos/metabolismo , Condrócitos/patologia , Modelos Animais , Músculo Quadríceps/metabolismo , Músculo Quadríceps/patologia , Cabeça do Fêmur/patologia
12.
Univ. med ; 57(4): 488-501, oct. - dic. 2016. ilus, tab
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1007307

RESUMO

Introduction: The anatomic diaphanization technique consists in the transparency of soft tissues of vertebrate organisms (clearing), in order to dye the mineralized tissue and visualize bone and cartilage (staining). Objective: To review the reports available on specialized literature that describes the embryonic development of mammal ́s skeletal system through the diaphonization technique and simple staining or double staining processes. Materials and methods: A literature review was made on PubMed, Academic Google and SciELO, based on PRISMA, through the association of the term double staining with the health descriptors of the Medical Subject Headings (MeSH) alizarin red, alician blue, bone and cartilage , and the combination of the Boolean operators + and &. Results: There were included 22 articles that described the diaphanization technique and the simple staining or double staining technique used in the observation, registration and analysis of the embryonic development of mammal ́s skeletal system. Conclusion: The diaphanization technique and simple staining or double staining technique is an anatomic study technique­versatile and of low cost­of the embryonic development of mammal ́s skeletal system, which may be used in toxicology studies, discarding the possibility of developmental anomalies during the cartilage or bone formation (chondrogenesis and osteogenesis, respectively) associated to the exposure to a possible teratogenic agent.


Assuntos
Humanos , Anatomia Comparada , Desenvolvimento Ósseo , Cartilagem , Estruturas Embrionárias
13.
Rev. biol. trop ; 63(1): 155-164, Jan.-Mar. 2015. ilus
Artigo em Espanhol | LILACS | ID: lil-753782

RESUMO

The spotted rose snapper (Lutjanus guttatus) is an important commercial species in Mexico with good culture potential. The osteological study at early stages in this species is an important tool to confirm normal bone structure and for the detection of malformations that may occur during early development. This study was carried out in order to evaluate and describe the normal osteological development of the vertebral column and caudal complex of this species grown under controlled conditions. For this, a total of 540 larvae of L. guttatus, between 2.1 and 17.5mm of total length (TL), were cultured during 36 days; culture conditions were 28ºC, 5.74mg/L oxygen and 32.2ups salinity with standard feeding rates. To detect growth changes, a sample of 15 organisms was daily taken from day one until day 36 of post-hatch (DPH). Samples were processed following standard techniques of clearing, and cartilage (alcian blue) and bone staining (alizarin red). Results showed that the vertebral column is composed of ten vertebrae in the abdominal region, and 14 vertebrae including the urostyle in the caudal region. The development of the axial skeleton starts with the neural arches and haemal arches at 3.8mm TL. Caudal elements such as the hypurals and parahypural began to develop at 4.1mm TL. Pre-flexion and flexion of the notochord and the formation of all hypurals were observed between 5.3 and 5.8mm TL. Ossification of the vertebrae in the abdominal region and in some neural arches initiated at 9.5mm TL. In the caudal region, all the neural and haemal arches ossified at 10.2mm TL. All the abdominal vertebrae and their respective neural arches and parapophyses ossified at 11.2mm TL, while the elements of the caudal complex that ossified were the hypurals, parahypurals and modified haemal spines. All caudal fin rays, 12 neural spines and 3 haemal arches were ossified by 15.5mm. The complete ossification process of this specie under laboratory culture conditions was observed when larvae reached 17.3mm TL on 36 DPH. Detailed analysis of the osteological structures will allow a reference description to evaluate and detect malformations that may occur during the larval culture of the spotted rose snapper.


El pargo flamenco (Lutjanus guttatus) es una especie de importancia comercial en México con un gran potencial para su cultivo. El estudio osteológico en estadios tempranos de esta especie bajo condiciones controladas, es una herramienta importante para el conocimiento de su estructura ósea normal y poder detectar las malformaciones que se puedan presentar. El objetivo del presente trabajo se realizó para conocer y describir el desarrollo osteológico normal de la columna vertebral y el complejo caudal de 540 larvas de 2.1 a 17.5mm de longitud total (LT) bajo condiciones de cultivo a 28°C, 5.74mg/L de oxígeno y 32.2UPS de salinidad. Diariamente se tomó una muestra de 15 organismos desde el día uno hasta el 36 después de la eclosión (DDE) y se procesaron con las técnicas de clareado y tinción de cartílago (azul aciano) y hueso (rojo alizarina) para llevar a cabo la descripción de las estructuras. La columna vertebral se divide en región abdominal con diez vértebras y región caudal compuesta por 14 vértebras incluido el urostilo. El desarrollo del esqueleto axial inicia con la formación de los arcos neurales y hemales a los 3.8mm de LT. A los 4.1mm de LT empieza la formación de los hipurales y parahipural que son elementos caudales. Entre los 5.3 y 5.8mm de LT se observó en pre-flexión y flexión del notocordio y la formación de todos los hipurales. La osificación de las vértebras en la región abdominal y en algunos arcos neurales inició a los 9.5mm de LT. A los 10.2mm de LT se osificó la región caudal y todos los arcos neurales y hemales. A los 11.2mm LT se osificaron todas las vértebras abdominales con sus respectivos arcos neurales y los parapófisis, mientras que los elementos del esqueleto caudal que se osificaron fueron los hipurales, parahipurales y las espinas hemales modificadas. A los 15.5mm de LT se osificaron los radios de la aleta caudal y 12 espinas neurales y 3 hemales. El proceso de osificación de las larvas de esta especie en condiciones de cultivo se completó a los 17.3mm LT o 36 DDE. El análisis detallado de las estructuras osteológicas, permitirá una descripción de referencia para evaluar y detectar las malformaciones que se puedan presentar durante el cultivo larvario.


Assuntos
Animais , Nadadeiras de Animais/embriologia , Cartilagem/embriologia , Osteogênese/fisiologia , Perciformes/embriologia , Coluna Vertebral/embriologia , Larva/crescimento & desenvolvimento , México
14.
Acta méd. costarric ; 57(1): 49-51, ene.-mar. 2015. ilus
Artigo em Espanhol | LILACS | ID: lil-753613

RESUMO

Se presenta el caso clínico de un niño de 1 año y 6 meses, atendido en el Hospital Nacional de Niños, por dificultad para deambular, que presentaba datos radiológicos de sobrecrecimiento osteocondral epifisiario de tibia proximal y distal, astrágalo y primer metatarsiano del miembro inferior izquierdo, y se diagnostica displasia epifisiaria hemimelia. La displasia epifisiaria hemimélica o enfermedad de Trevor, es un defecto osteocartilaginoso, no hereditario. Dicha condición afecta las epífisis y centros de osificación, y se observa principalmente a nivel de las extremidades inferiores. La prevalencia global es de uno en un millón y su etiología es desconocida. Es tres veces más común en varones que en mujeres. El diagnóstico es radiológico y el manejo puede ser conservador o quirúrgico.


We describe the clinical case of an 18-month old boy, evaluated at the National Children’s Hospital for difficulties in gait, with radiological findings of osteochondral overgrowth of the epiphysis of the proximal and distal tibia, astragalus and first metatarsal bone of the left leg. The patient was diagnosed with epiphyseal dysplasia hemimelica. Epiphyseal dysplasia hemimelica or Trevor´s disease is a non-hereditary osteo-cartilaginous defect. It affects the epiphysis and ossification centers, occurring predominantly in the lower limbs. Global prevalence is one in a million and its etiology is unknown. It is three times more common in boys than girls. Diagnosis is made through radiologic studies. Management options include clinical observation or surgery.


Assuntos
Humanos , Masculino , Criança , Cartilagem , Epífises , Extremidade Inferior , Esqueleto
15.
Acta otorrinolaringol. cir. cabeza cuello ; 43(4): 277-280, 20150000. ilus
Artigo em Espanhol | LILACS | ID: biblio-966452

RESUMO

Introducción: El uso de injertos autólogos de cartílago en rinoplastia primaria se encuentra reportado ampliamente en la literatura, al igual que los diferentes sitios donantes de donde se pueden obtener. En el presente artículo se propone el uso del dorso cartilaginoso como sitio donante para tallar injertos de cartílago, siendo esta, una opción diferente y eficaz para la corrección de diversas deformidades nasales. Objetivo: Reportar la experiencia con el uso de cartilago del dorso cartilaginoso como fuente de material autólogo suficiente y adecuado para injertos en rinoplastia primaria. Diseño: Reporte de caso. Materiales y métodos: Descripción detallada de caso clínico, planeamiento y técnica quirúrgica del autor principal. Resultados: Paciente de 23 años de edad, género femenino con giba dorsal prominente y una punta nasal ptosica. Al examen estético nasal se encuentra una giba de tipo osteocartilaginosa, con la punta nasal con pobre definición, subproyectada e hiporotada. Se realizó el procedimiento quirúrgico corrigiendo exitosamente las deformidades nasales obteniendo una paciente satisfecha. Conclusiones: Es posible aprovechar el cartílago disponible en pacientes que presentan una giba cartilaginosa y requieren injertos para la corrección de las deformidades nasales, evidenciando beneficios como evitar incisiones adicionales o la preservación del cartílago septal y auricular.


Introduction: The use of autologous cartilage grafts in primary rhinoplasty is widely reported in the literature, as well as the different donor sites. In the present article the use of cartilaginous dorsum as donor site is proposed to carve grafts cartilage, this proposal is a different and effective option for correction of various nasal deformities. Objective: To report the experience with the use of cartilaginous dorsum as a source of sufficient and suitable autologous grafts in primary rhinoplasty. Design: Case report. Methodology: Detailed description of a clinical case, planning and surgical technique of the experienced lead author. Results: Female 23 year old patient with a prominent dorsal hump and ptotic nasal tip. In the aesthetic nasal examination there is a cartilaginous hump, with poorly nasal tip definition, sub-projected and underrotated nasal tip. The surgical procedure was successfully performed with correcting nasal deformities and obtaining a satisfied patient. Conclusions: It is feasible to use cartilage available in patients with a cartilaginous hump and that require grafting for correction of nasal deformities, showing benefits such as avoiding additional incisions or preserving the septal and aural cartilage.


Assuntos
Rinoplastia , Cartilagem , Deformidades Adquiridas Nasais , Autoenxertos
16.
Anon.
Acta ortop. mex ; 28(6): 378-381, nov.-dic. 2014. ilus
Artigo em Espanhol | LILACS | ID: lil-745200

RESUMO

El condroblastoma de la cabeza femoral es un tumor poco frecuente (aproximadamente 1 o 2% de los tumores benignos de hueso), se presenta con mayor frecuencia en pacientes jóvenes de sexo masculino. El condroblastoma es más frecuente en el húmero proximal, fémur proximal, fémur distal y tibia proximal. La cabeza femoral es el tercer sitio más frecuente, No existe un tratamiento específico para esta entidad; se han descrito desde osteotomías acetabulares, injertos osteocondrales, injertos vascularizados de peroné, etcétera, todos reportando buenos resultados. Pero es clínicamente impredecible si no recibe tratamiento. Se presenta un caso que se manejó con aloinjerto osteocondral y la evolución de tres años posterior al procedimiento quirúrgico...


Femoral head chondroblastoma is an infrequent tumor, accounting for approximately 1-2% of benign bone tumors. It occurs more frequently in young male patients. It's most frequent locations include the proximal humerus, proximal femur, distal femur and proximal tibia. The femoral head is the third most frequent site of this tumor. There is no specific treatment for this entity; reported treatments range from acetabular osteotomies and osteochondral grafts, to vascularized fibular grafts, all of them with good results. However, this tumor is clinically unpredictable if left untreated. We report a case managed with osteochondral graft and followed-up for three years after the surgical procedure...


Assuntos
Adolescente , Humanos , Masculino , Condroblastoma/cirurgia , Cabeça do Fêmur , Neoplasias Femorais/cirurgia , Aloenxertos , Transplante Ósseo , Cartilagem/transplante
17.
Braz. j. otorhinolaryngol. (Impr.) ; 80(6): 522-526, Nov-Dec/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-730455

RESUMO

Introduction: Cartilage graft tympanoplasty has a better success rate in the treatment of chronic otitis media if regularly prepared and placed. Objective: To prepare cartilage island material and evaluate its effect on the success rate of tympanoplasty. Methods: The medical records of 87 patients (48 males and 39 females; mean age, 27.3 ±11.2 years; range, 14–43 years) with chronic otitis media without cholesteatoma who underwent intact canal-wall-up tympanoplasty and revision surgery between December of 2007 and October of 2011 were retrospectively evaluated. Surgery was performed under general anesthesia via a retroauricular approach. Results: The overall success rate of this technique was 93% in terms of perforation closure. No graft lateralization or displacement into the middle ear occurred. The overall average preoperative air bone gap was 37.27 ± 12.35 dB, and the postoperative air bone gap was 27.58 ± 9.84 dB. The mean postoperative follow-up period was 15.3 months (range: 7–21 months). Conclusion: If cartilage graft is properly prepared and placed, cartilage graft tympanoplasty appears to provide better success rates and hearing results. .


Introducão: A timpanoplastia de enxerto de cartilagem tem uma melhor taxa de sucesso no tratamento de otite média crónica se for preparada e colocada de forma sistemática. Objetivo: Preparar o material de enxerto de cartilagem “em ilha" e avaliar o seu impacto na taxa de sucesso da timpanoplastia. Método: Os registos médicos de 87 pacientes (48 do sexo masculino e 39 do sexo feminino; idade média 27.3 ±11.2 anos; intervalo 14–43 anos) com otite média crónica sem colesteatoma que foram submetidos a timpanoplastia de levantamento de parede do canal intacto e cirurgia de revisão entre Dezembro 2007 e Outubro 2011 foram avaliados em retrospetiva. A cirurgia foi realizada sob anestesia geral através de uma abordagem retro auricular. Resultados: A taxa de sucesso global da nossa técnica foi de 93% em termos de sutura da perfuração. Não ocorreu lateralização do enxerto nem deslocação para o ouvido médio. O intervalo aéreo-ósseo pré-operatório médio global (ABG) era de 37.27 ±12.35 dB e o ABG pós-operatório era de 27.58±9.84dB. O período médio de seguimento pós-operatório era de 15.3 meses (intervalo 7-21 meses). Conclusão: Se o enxerto de cartilagem for devidamente preparado e colocado, a Timpanoplastia de enxerto de cartilagem apresenta melhores taxas de sucesso e resultados ao nível da audição. .


Assuntos
Adolescente , Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Cartilagem/transplante , Otite Média/cirurgia , Perfuração da Membrana Timpânica/cirurgia , Timpanoplastia/métodos , Otite Média/complicações , Estudos Retrospectivos , Resultado do Tratamento , Perfuração da Membrana Timpânica/etiologia
18.
Braz. j. med. biol. res ; 47(7): 533-539, 07/2014. tab
Artigo em Inglês | LILACS | ID: lil-712964

RESUMO

Development and selection of an ideal scaffold is of importance for tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a biocompatible bioresorbable copolymer that belongs to the polyhydroxyalkanoate family. Because of its good biocompatibility, PHBHHx has been widely used as a cell scaffold for tissue engineering. This review focuses on the utilization of PHBHHx-based scaffolds in tissue engineering. Advances in the preparation, modification, and application of PHBHHx scaffolds are discussed.


Assuntos
Humanos , /química , Materiais Biocompatíveis/química , Caproatos/química , Engenharia Tecidual/métodos , Tecidos Suporte/química , /uso terapêutico , Materiais Biocompatíveis/uso terapêutico , Osso e Ossos/fisiologia , Caproatos/uso terapêutico , Cartilagem/fisiologia , Liofilização , Músculo Liso/fisiologia , Regeneração , Propriedades de Superfície
19.
Acta ortop. bras ; 22(4): 202-205, Jul-Aug/2014. ilus, tab
Artigo em Inglês, Português | LILACS | ID: lil-784746

RESUMO

Verificar a celularidade e espessura da cartilagem articulardo fêmur em ratos com artrite após terapia por iontoforese.Métodos: Para avaliar estes objetivos, uma análise histológicafoi realizada em hematoxilina e eosina, onde a celularidade e asespessuras da cartilagem foram observadas e avaliadas qualitativae quantitativamente por contagem manual por área de700.09 μm². Resultados: O grupo tratado com IAA obteve normalidadede celularidade (40,1 células/µm²) e manutenção da cartilagemnão calcificada (75,5 μm), sugerindo espessura normal. Ogrupo não tratado C+, por outro lado, apresentou menor médiado número de condrócitos (13.0 µm²), (P <0,05), e em relação àespessura da cartilagem mostrou maior média de cartilagem calcificadacom espessura (104,8 μm) e menor média de cartilagemnão calcificada (53,3 µm) Conclusão: A terapia da iontoforese comL-ácido ascórbico através da corrente elétrica continua contribuiupara um ganho quantitativo de condrócitos e melhorou a distribui-ção de espessuras da cartilagem calcificadas e não calcificadas.Nível de Evidência III, Estudo de Caso Controle...


To examine the cellularity and thickness of the articularcartilage of the femur in rats with arthritis after treatment withiontophoresis. Methods: To evaluate these objectives, a histologicalanalysis was performed on hematoxylin and eosin, wherecellularity and cartilage thickness were observed and evaluatedqualitatively and quantitatively by manual counting by 700.09 μm²area. Results: The group treated with IAA had normal cellularity(40.1 cells/μm2) and maintenance of non-calcified cartilage(75.5 μm), suggesting normal thickness. The non-treated groupC+, on the other hand, had a lower mean number of chondrocytes(13.0 μm2) (P <0.05) and, when the cartilage thickness was compared,it showed higher average thickness of calcified cartilage(104.8 μm) and lower mean of non-calcified cartilage (53.3 μm)Conclusion: The use of iontophoresis with L-ascorbic acid bythe continuous electric current contributed to a quantitative gainof chondrocytes and improved the thickness distribution of calcifiedand non-calcified cartilage. Level of Evidence III, CaseControl Study...


Assuntos
Ratos , Artrite , Cartilagem , Iontoforese , Osteoartrite , Ácido Ascórbico
20.
Braz. j. med. biol. res ; 47(6): 445-451, 06/2014. graf
Artigo em Inglês | LILACS | ID: lil-709443

RESUMO

Current studies find that degenerated cartilage endplates (CEP) of vertebrae, with fewer diffusion areas, decrease nutrient supply and accelerate intervertebral disc degeneration. Many more apoptotic cells have been identified in degenerated than in normal endplates, and may be responsible for the degenerated grade. Previous findings suggest that inhibition of apoptosis is one possible approach to improve disc regeneration. It is postulated that inhibition of CEP cell apoptosis may be responsible for the regeneration of endplates. Caspase-3, involved in the execution phase of apoptosis, is a candidate for regulating the apoptotic process. In the present study, CEP cells were incubated in 1% fetal bovine serum. Activated caspases were detected to identify the apoptotic pathway, and apoptosis was quantified by flow cytometry. Lentiviral caspase-3 short hairpin RNA (shRNA) was employed to study its protective effects against serum deprivation. Silencing of caspase-3 expression was quantified by reverse transcription-polymerase chain reaction and Western blots, and inhibition of apoptosis was quantified by flow cytometry. Serum deprivation increased apoptosis of rat CEP cells through activation of a caspase cascade. Lentiviral caspase-3 shRNA was successfully transduced into CEP cells, and specifically silenced endogenous caspase-3 expression. Surviving cells were protected by the downregulation of caspase-3 expression and activation. Thus, lentiviral caspase-3 shRNA-mediated RNAi successfully silenced endogenous caspase-3 expression, preventing inappropriate or premature apoptosis.


Assuntos
Animais , Bovinos , Apoptose/fisiologia , /metabolismo , Condrócitos/metabolismo , Lentivirus/genética , Interferência de RNA/fisiologia , Inanição/metabolismo , Western Blotting , Cartilagem/metabolismo , Caspase 9/metabolismo , /metabolismo , Citometria de Fluxo , Vetores Genéticos/metabolismo , Microscopia de Fluorescência , Cultura Primária de Células , Propídio , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Soro/fisiologia , Transfecção
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