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1.
Dement. neuropsychol ; 14(1): 35-40, Jan.-Mar. 2020. tab
Artigo em Inglês | LILACS | ID: biblio-1089807

RESUMO

ABSTRACT Currently, the most likely hypotheses as the cause of Alzheimer's disease are deposition of amyloid beta peptide in the cerebral cortex and hyperphosphorylation of Tau protein. The diagnosis of Alzheimer's disease is based on the exclusion of other diseases, behavioral assessments, and blood and imaging tests. Biotechnology has created interesting perspectives for the early detection of Alzheimer's disease through blood analysis, with special attention to platelets, hemoglobin and vitamin B12. Objective: To evaluate the concentrations of platelets, hemoglobin and vitamin B12 in the blood of older adults with and without dementia of Alzheimer's disease. Methods: A case-control study involving 120 individuals was conducted, seeking to establish a correlation between changes in platelet, hemoglobin and vitamin B12 concentrations in patients with confirmed AD and in individuals in the inclusion group without AD. The study met the established ethical requirements. Results: Hemoglobin and platelet levels were statistically lower in patients with AD. The biochemical evaluation in AD patient and healthy groups for vitamin B12 showed a decrease in the levels of this compound in patients with AD. Conclusion: We demonstrated the feasibility of the use of blood biomarkers as predictive markers for the diagnosis of AD.


RESUMO Atualmente, as hipóteses mais prováveis como causa da doença de Alzheimer são a deposição do peptídeo beta amiloide no córtex cerebral e a hiperfosforilação da proteína Tau. O diagnóstico da doença de Alzheimer baseia-se na exclusão de outras doenças, avaliações comportamentais e exames de imagem e sangue. A biotecnologia criou perspectivas interessantes para a detecção precoce da doença de Alzheimer, pela análise sanguínea, com atenção especial às plaquetas, hemoglobina e vitamina B12. Objetivo: Avaliar as concentrações de plaquetas, hemoglobina e vitamina B12 no sangue de idosos com e sem demência de Alzheimer. Métodos: O estudo de caso-controle envolveu 120 indivíduos, buscando correlação entre mudanças nas concentrações de plaquetas, hemoglobina e vitamina B12 em pacientes com DA confirmada e indivíduos do grupo de inclusão, sem DA. Resultados: Os níveis de hemoglobina e plaquetas são estatisticamente mais baixos em pacientes com DA. A avaliação bioquímica em pacientes com DA e grupos saudáveis para vitamina B12 mostrou uma diminuição nos níveis deste composto em pacientes com DA. Conclusão: Demonstramos a viabilidade do uso de biomarcadores sanguíneos como marcadores preditivos para o diagnóstico de DA.


Assuntos
Humanos , Vitamina B 12 , Plaquetas , Hemoglobinas , Biomarcadores , Demência , Doença de Alzheimer
2.
Rev. ADM ; 77(1): 28-36, ene.-feb. 2020. tab
Artigo em Espanhol | LILACS | ID: biblio-1087974

RESUMO

Las alteraciones hematológicas pueden tener el primer signo en la cavidad oral y los signos varían dependiendo de la línea celular que se encuentre afectada: eritrocitos, leucocitos y plaquetas. La formación de las células sanguíneas se lleva a cabo en la médula ósea a través de un proceso denominado hematopoyesis que se encarga de la formación, desarrollo y especialización de todas sus células sanguíneas funcionales, pasan de células troncales pluripotenciales a células hematopoyéticas maduras que emergen a la sangre periférica. El odontólogo debe ser capaz de identificar los distintos signos en la cavidad oral que podrían sugerir que el paciente padece un trastorno hematológico, el cual podría complicar el tratamiento dental. La identificación oportuna de estos signos a través de una minuciosa exploración física y la historia clínica completa evita que se presenten complicaciones en el paciente y que éstas puedan poner en riesgo su vida, por lo que al encontrar algún signo sugerente de un trastorno hematológico debe referirse al paciente con el hematólogo (AU)


Hematological alterations may be the first sign in the oral cavity and symptoms vary depending on the cell line that is affected: Erythrocytes, leukocytes and platelets. The formation of blood cells are held in the bone marrow through a process called hematopoiesis, which is responsible for training, development and specialization in all its functional blood cells, they move from pluripotent stem cell to hematopoietic cells mature emerging to peripheral blood. The dentist must be able to identify the different signs in the oral cavity that could suggest that the patient has a haematological disorder, which could complicate dental treatment. The timely identification of these signs through a thorough physical examination and the complete clinical history prevents complications from occurring in the patient and may put their lives at risk, so when finding any sign suggestive of a hematological disorder should refer to the patient with the hematologist (AU)


Assuntos
Humanos , Manifestações Bucais , Transtornos da Coagulação Sanguínea/classificação , Doenças Hematológicas , Doenças Periodontais , Plaquetas , Assistência Odontológica para Doentes Crônicos , Úlceras Orais , Eritrócitos , Leucócitos
3.
Acta cir. bras ; 34(7): e201900710, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1038119

RESUMO

Abstract Purpose: To investigate the prognostic value of 17 platelet-based prognostic scores in patients with malignant hepatic tumors after TACE therapy. Methods: In total, 92 patients were divided into death group and survival group according to long-term follow-up results. The AUC was calculated to determine the optimal cut-off values for predicting prognosis. To determine better prognostic models, platelet-based models were analyzed separately after being showed as binary according to cut-off values. Cumulative survival rates of malignant hepatic tumors were calculated using Kaplan-Meier curves and differences were analyzed by the log-rank test. Univariate and multivariate analyses were performed to identify platelet-based prognostic scores associated with overall survival. Results: Univariate analysis showed that APGA, APRI, FIB-4, FibroQ, GUCI, King's score, Lok index, PAPAS, cirrhosis, number of tumors, vascular cancer embolus, AFP, ALP and APTT were significantly related to prognosis. A multivariate analysis showed that the APGA, number of tumors, ALP and APTT were independently associated with overall survival. Conclusion: This study showed that the APGA, a platelet-based prognostic score, was an independent marker of prognosis in patients with malignant hepatic tumors after TACE and was superior to the other platelet-based prognostic scores in terms of prognostic ability.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Aspartato Aminotransferases/sangue , Plaquetas/química , Quimioembolização Terapêutica/métodos , Carcinoma Hepatocelular/terapia , Neoplasias Hepáticas/terapia , Contagem de Plaquetas , Prognóstico , Biomarcadores Tumorais/sangue , Estudos Retrospectivos , Curva ROC , Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/sangue , Estimativa de Kaplan-Meier , Neoplasias Hepáticas/mortalidade , Neoplasias Hepáticas/sangue
4.
Araçatuba; s.n; 2019. 88 p. graf, ilus, tab.
Tese em Português | LILACS, BBO - Odontologia | ID: biblio-1051384

RESUMO

O objetivo deste estudo foi avaliar a influência da Fibrina Rica em Plaqueta (FRP) no processo inflamatório em defeitos críticos em calotas de ratos e sua consequente reparação tecidual. Foram utilizados 128 (cento e vinte e oito) ratos (Rattus norvegicus, albinus, Wistar), adultos, com peso corporal entre 450 e 500g. Os animais foram divididos aleatoriamente em 4 grupos equitativos que compuseram a amostra do trabalho, onde no grupo coágulo (GC) foi realizado o defeito ósseo de tamanho crítico preenchido com coágulo sanguíneo; grupo anti-inflamatório não esteroidal (AINE) que teve os defeitos de tamanho crítico preenchidos com coágulo sanguíneo e administrado cetoprofeno (10mg/kg dia); o grupo fibrina rica em plaquetas (FRP) com defeitos de tamanho crítico preenchidos com preparado de fibrina rica em plaquetas autóloga; e o grupo fibrina rica em plaquetas mais AINE (FRP + AINE) com defeitos de tamanho crítico preenchidos com preparado de fibrina rica em plaquetas autóloga e administrado cetoprofeno (10mg/kg dia). Cada grupo foi avaliado nos períodos de 2, 7, 14 e 28 dias e os espécimes analisados através da histometria, micro-CT e teste ELISA para presença de TNFα. Os dados quantitativos foram submetidos aos testes estatísticos ANOVA 1/2 fatores ou Kruskal-Wallis e pos Tukey e Dunn (p<0,01). Os resultados histométricos e microtomográficos evidenciaram maior formação óssea para o grupo PRF em comparação aos demais grupos (p<0,05) e menor presença de TNF-alfa no período inicial no grupo PRF comparado ao grupo controle (p<0,05). Conclui-se que o PRF foi favorável desde os períodos iniciais até os mas tardios, auxiliando na resposta inflamatória e neoformação óssea(AU)


The objective of this study was to evaluate the influence of Platelet Rich Fibrin (PRF) on the inflammatory process in critical defects in rat calvaria and its consequent tissue repair. One hundred twenty-eight adult rats (Rattus norvegicus, albinus, Wistar) with body weight between 450 and 500g were used for the study. The animals were randomly divided into 4 equitable groups that composed the work sample. In the clot group (GC) the critical size defect was filled with blood clot; the non-steroidal anti-inflammatory group (NSAID) had the critical size defects filled with blood clot and ketoprofen was given pos-operatively (10mg / kg day); the platelet-rich fibrin group (FRP) had the critical-size defects filled with autologous platelet-rich fibrin preparation; and platelet-rich fibrina group plus NSAIDs (FRP + NSAID) had the critical-size defects filled with autologous platelet-rich fibrin preparation and administered ketoprofen post-operatively (10mg / kg day). Each group was evaluated at 2, 7, 14 and 28 days after the surgical procedures. The samples were evaluated through histometry, micro-CT and ELISA for the presence of TNFα. The quantitative data were submitted to the statistical test ANOVA 1/2 factors or Kruskal-Wallis and post Tukey and Dunn (p <0.01). The histometric and microtomographic results showed higher bone formation for the PRF group compared to the other groups (p <0.05) and lower TNF-alpha in the initial period in the PRF group compared to the control group (p <0.05). It was concluded that the PRF was favorable since the beginning through the later periods, aiding in the inflammatory response and bone neoformation(AU)


Assuntos
Animais , Ratos , Regeneração Óssea , Anti-Inflamatórios não Esteroides , Fibrina Rica em Plaquetas , Células Sanguíneas , Plaquetas , Ratos Wistar , Inflamação
5.
Rev. bras. anal. clin ; 50(2): 174-178, nov. 23, 2018. tab, graf
Artigo em Português | LILACS | ID: biblio-963796

RESUMO

Objetivo: Comparar resultados de contagens plaquetárias de indivíduos hospitalizados realizadas por impedância (PLT-I) e metodologia óptica fluorescente (PLT-O). Métodos: Em estudo retrospectivo, foram avaliados dados sequenciais arquivados de contagens plaquetárias de trezentos indivíduos adultos hospitalizados, incluindo casos de anemias microcíticas e hemolíticas, neoplasias hematológicas, entre outras doenças. Todos os casos continham contagens de plaquetas PLT-I e PLT-O realizadas no equipamento Sysmex XE-5000. Resultados: Não houve diferença significativa entre os valores de contagens plaquetárias entre a PLT-I e PLT-O (p=0,614). Quando avaliamos os valores de plaquetas entre diferentes grupos em relação às metodologias, não houve diferença entre as contagens plaquetárias naqueles com VCM abaixo de 80 fL (p=0,936), VCM abaixo de 70 (p=0,821), plaquetas abaixo de 100×109/L (p=0,369) e plaquetas abaixo de 50×109/L (p=0,314). Além disso, a correlação entre PLT-I e PLT-O foi forte. Conclusão: Os valores de contagens plaquetárias, provenientes de pacientes não saudáveis, realizadas no analisador XE-5000 pelos métodos óptico e impedância, mostraram forte correlação e boa concordância.


Assuntos
Plaquetas , Contagem de Plaquetas , Impedância Elétrica , Pacientes Internados
6.
Rev. bras. anal. clin ; 50(2): 171-173, nov. 23, 2018. tab
Artigo em Português | LILACS | ID: biblio-963787

RESUMO

Objetivo: O objetivo desse estudo foi elaborar os valores de referência desses parâmetros, baseado na casuística do Laboratório de Análises Clínicas Veterinárias (LaClin) da Universidade Federal de Santa Catarina para a microrregião de Curitibanos. Métodos: Avaliaram-se os novos índices plaquetários MPV, PDW e PCT de 100 caninos (41 machos e 59 fêmeas) e 40 felinos (13 machos e 27 fêmeas) saudáveis, atendidos no período de junho de 2015 até agosto de 2017 na faculdade de Medicina Veterinária da UFSC, na microrregião de Curitibanos, Santa Catarina. O valor de referência para MPV e PDW foi elaborado de acordo com a média dos valores obtidos dos animais avaliados ± 2 desvios-padrão, e, para PCT utilizou-se ± 1 DP. Resultados: Os valores de referência de MPV(fL), PDW(%) e PCT(%) [média (limite inferior ­ limite superior)] propostos para caninos foram, respectivamente 9,08 (7,44 -10,71), 16,07 (15,32 -16,82) e 0,32 (0,21-0,42), e para felinos, respectivamente 9,90 (8,07-11,74), 16,26 (15,21-17,32) e 0,24 (0,11-0,37). Conclusão: Os valores encontrados no presente estudo não condizem com outros estudos ou nunca foram investigados.


Assuntos
Plaquetas , Animais Domésticos , Contagem de Plaquetas , Volume Plaquetário Médio
8.
Rev. méd. hered ; 29(2): 116-119, abr. 2018. []
Artigo em Espanhol | LILACS, LIPECS | ID: biblio-1014303

RESUMO

La sepsis es una de las causas más frecuentes que motivan el ingreso de pacientes al hospital y a las unidades de cuidados intensivos, tiene alto impacto en la morbilidad y mortalidad. Pese a los avances tecnológicos que han permitido mejorar el soporte de fallos orgánicos, no se ha logrado disminuir de forma importante sus complicaciones. Por ello es importante tener herramientas que sean predictoras de severidad en esta patología; los biomarcadores (procalcitonina, pro adrenomodulina, interleuquina 6), se han convertido en buenos índices de pronostico y evolución, sin embargo, su disponibilidad ya sea por el costo o existencia de reactivos en los distintos laboratorios no es constante; es por ello que buscamos con ésta revisión determinar si el uso del volumen medio plaquetario, un biomarcador reportado en el hemograma común y que se viene usando con relativo éxito en patologías cardiovascular, metabólica e inflamatoria/infecciosa, como un indicador de pronóstico de severidad y mortalidad. (AU)


Sepsis is one of the most common causes of hospital admission to intensive care units and has a high impact on morbidity and mortality. In spite of the technological advances that have allowed to improve the support of organic failures, it has not been possible to diminish of important form its complications. Therefore, it is important to have tools that are predictive of severity in this pathology, the biomarkers (procalcitin, proadrenomodulin, interleukin 6) have become good indicators of prognosis and evolution, however their availability either by cost or existence of reagents in the different laboratories is not constant; this is why we seek with this review to determine if determine if the use of mean platelet volume, a biomarker reported in the common hemogram and has been used with relative success in cardiovascular, metabolic and inflammatory / infectious pathology, as an indicator of prognosis of severity and mortality. (AU)


Assuntos
Humanos , Plaquetas , Biomarcadores , Sepse/mortalidade , Volume Plaquetário Médio/mortalidade
10.
Arch. argent. pediatr ; 115(6): 576-580, dic. 2017. tab
Artigo em Inglês, Espanhol | LILACS, BINACIS | ID: biblio-1038392

RESUMO

Objetivo.Evaluar la relación entre las variables de volumen plaquetario medio (VPM), índice de neutrófilos/linfocitos (INL) e índice de trombocitos/linfocitos (ITL) y el diagnóstico o la predicción del desenlace en los niños con intoxicación por mordedura de serpiente. Métodos.Se realizó una evaluación retrospectiva de niños con diagnóstico de intoxicación por mordedura de serpiente y un grupo de referencia de sujetos sanos. Se clasificó a los pacientes en tres grupos de intoxicación: leve, moderada y grave. Resultados.Se incluyeron 142 niños en el estudio. La leucocitosis (p= 0, 003), la neutrofilia (p= 0, 026) y la trombocitopenia (p= 0, 034) fueron significativamente más frecuentes en los casos de intoxicación por mordedura de serpiente grave; sin embargo, no se hallaron diferencias estadísticamente significativas en relación con el VPM, el INL y el ITL entre los diferentes grupos de intoxicación por mordedura de serpiente. La media del VPM, el INL y el ITL era significativamente mayor entre los niños con mordedura de serpiente en comparación con los controles sanos. Conclusiones.Según nuestros resultados, el uso del VPM, el INL y el ITL podría servir para el diagnóstico como marcadores inflamatorios en los casos de intoxicación por mordedura de serpiente.


Background: The objective of this study is to evaluate the relationships between the mean platelet volume (MPV), neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) variables and diagnosis or prediction of outcome in children with snakebite envenomation. Methods: Children diagnosed with snakebite envenomation and a control group of healthy subjects were retrospectively evaluated. Patients were classified into three groups as mild, moderate and severe. Results: 142 children were enrolled in the study. Leukocytosis (p= 0.003), neutrophilia (p= 0.026) and thrombocytopenia (p= 0.034) were significantly more common in severe snakebite envenomation, although no statistical significant were found in association with MPV, NLR and PLR between snakebite envenomation groups. The mean MPV, NLR and PLR were found to be significantly higher in children with snakebite compared to than among healthy controls. Conclusions: Our results suggested that MPV, NLR and PLR may be useful for the diagnosis as inflammatory markers in snakebite envenomation.


Assuntos
Humanos , Pré-Escolar , Criança , Adolescente , Envenenamento , Serpentes , Plaquetas , Linfócitos , Criança , Índice , Volume Plaquetário Médio , Neutrófilos
11.
Rev. cuba. hematol. inmunol. hemoter ; 33(3): 42-54, jul.-set. 2017.
Artigo em Espanhol | LILACS | ID: biblio-960420

RESUMO

La trombocitopenia puede tener varias causas, como la utilización de determinados fármacos. Los mecanismos causantes de la trombocitopenia inducida por fármacos incluyen disminución en la producción (supresión medular) o incremento en la destrucción (por mecanismos inmunes). Adicionalmente, la seudotrombocitopenia es un efecto in vitro, que se distingue de una real trombocitopenia inducida por medicamentos. Los estudios epidemiológicos son pocos, difieren en la metodología utilizada y describen una incidencia de 10 casos por millón de habitantes por año. El mecanismo fundamental de la trombocitopenia inducida por fármacos no está completamente esclarecido, pero al menos se plantean seis posibles mecanismos: anticuerpos inducidos por haptenos, anticuerpos dependientes del fármaco, inhibidores del complejo GP IIb-IIIa, autoanticuerpos inducidos por la droga, complejos inmunes y trombocitopenia inducida por heparina. La diana para los anticuerpos dependientes del fármaco son las glucoproteínas de la membrana plaquetaria, como las glucoproteínas Ib/IX y GPIIb/IIIa. El diagnóstico de trombocitopenia inducida por fármacos puede consistir en la identificación de síntomas clínicos (hematomas, petequias, sangramientos), la cuidadosa evaluación de la relación causal con el fármaco sospechoso, las investigaciones generales de laboratorio (conteos en sangre total, extendidos de sangre periférica, para descartar seudotrombocitopenia) y las pruebas serológicas para plaquetas. La trombocitopenia inducida por fármacos es una reacción adversa a medicamentos relativamente raros cuyas sus consecuencias pueden ser graves.


Thrombocytopenia can have several causes, including the use of certain drugs. The mechanism behind drug-induced thrombocytopenia is either a decrease in platelet production (bone marrow suppression) or an increased destruction (immune-mediated thrombocytopenia). In addition, pseudothrombocytopenia, an in vitro effect, has to be distinguished from true drug-induced thrombocytopenia. A small number of epidemiological studies, differing largely in the methodology used, describe incidences in the magnitude of 10 cases per 1 000 000 inhabitants per year. The underlying mechanism of drug-induced immune thrombocytopenia is not completely clarified, but at least six different types of antibodies appear to play a role; hapten-induced antibody, drug-dependent antibody ("compound" or "conformational-dependent" antibody), GPIIb-IIIa inhibitors, drug-induced autoantibody, immune complex and heparin-induced thrombocytopenia. Targets for drug-dependent antibodies are glycoproteins on the cell membrane of the platelets, such as glycoprotein (GP) Ib/IX and GPIIb/IIIa. Diagnosis of drug-induced immune thrombocytopenia may consist of identifying clinical symptoms (bruising, petechiae, bleeding), a careful evaluation of the causal relationship of the suspected causative drug, general laboratory investigation, such as total blood count and peripheral blood smear (to rule out pseudothrombocytopenia), and platelet serology tests. Although drug-induced thrombocytopenia is a relatively rare adverse drug reaction, its consequences may be severe.


Assuntos
Humanos , Masculino , Feminino , Trombocitopenia/induzido quimicamente , Trombocitopenia/epidemiologia , Plaquetas , Diagnóstico Diferencial
12.
Int. j. cardiovasc. sci. (Impr.) ; 30(5): f:442-l:451, set.-out. 2017. tab, graf
Artigo em Português | LILACS | ID: biblio-859031

RESUMO

Em condições de equilíbrio, a hemostasia é mantida através de uma complexa interação entre endotélio, plaquetas e fatores de coagulação. Situações que cursam com injúria e descontinuidade do revestimento endotelial estimulam a adesão, ativação e agregação de plaquetas, culminando com a formação de trombos arteriais ou venosos. Neste contexto, a terapia antiplaquetária ocupa um papel de destaque no manejo das patologias advindas deste processo, notadamente as síndromes coronarianas agudas.O maior domínio conceitual dos receptores, agonistas e antagonistas das cascatas fisiopatológicas envolvidasneste processo possibilitou o desenvolvimento de novos fármacos e o refinamento da terapêutica atual, tornando necessário o pleno conhecimento do arsenal antiplaquetário no que tange à sua indicação, posologia, momento de administração e duração do tratamento. O objetivo desta revisão é definir o papel dos fármacos antiplaquetários no manuseio da síndrome coronariana aguda, revisitando aspectos já consolidados e abordando tópicos atuais e ainda controversos acerca do tema


Assuntos
Humanos , Síndrome Coronariana Aguda/fisiopatologia , Inibidores da Agregação de Plaquetas/administração & dosagem , Anticoagulantes , Aspirina/administração & dosagem , Plaquetas , Infarto do Miocárdio/fisiopatologia , Intervenção Coronária Percutânea/métodos , Cloridrato de Prasugrel/administração & dosagem , Prognóstico , Terapia Trombolítica/métodos
13.
Rev. bras. hematol. hemoter ; 39(2): 122-126, Apr.-June 2017. tab
Artigo em Inglês | LILACS | ID: biblio-898916

RESUMO

ABSTRACT Background: Immune thrombocytopenia is an immune disease characterized by thrombocytopenia and bleeding due to platelet antibodies against platelet membrane glycoproteins. Human platelet antigens are derived from polymorphisms of these glycoproteins. The aim of this study was to investigate human platelet antigen frequencies in immune thrombocytopenia patients from the state of Amazonas, Brazil and investigate the potential association between specific antigens and risk for immune thrombocytopenia. Method: Human platelet antigen typing was performed by BeadChip technology to determine allelic variants of 11 systems (HPA-1 to HPA-9, HPA-11 and HPA-15). Thirty-six patients (8 male and 28 female) with a median age of 34 years (range: 9-69 years) were evaluated and compared with data from Amazonas blood donors. Results: Platelet counts varied from 3 to 98 × 109/L. The allele frequencies were 0.944 for HPA-1a, 0.056 for HPA-1b, 0.847 for HPA-2a, 0.153 for HPA-2b, 0.555 for HPA-3a, 0.444 for HPA-3b, 0.805 for HPA-5a, 0.222 for HPA-5b, 0.9975 for HPA-9a, 0.025 for HPA-9b, 0.486 for HPA-15a and 0.513 for HPA-15b. Among immune thrombocytopenia individuals, no b allele of the HPA-4, -6, -7, -8 and -11 were found. Conclusions: The results suggest HPA-1a, HPA-3b and HPA-5b are immune thrombocytopenia-specific autoepitopes.


Assuntos
Humanos , Masculino , Feminino , Plaquetas , Púrpura Trombocitopênica Idiopática , Antígenos de Plaquetas Humanas , Tipagem Molecular , Técnicas de Genotipagem
14.
Rev. bras. ciênc. vet ; 24(2): 77-80, abr.-jun./2017. il.
Artigo em Inglês | LILACS | ID: biblio-966338

RESUMO

O objetivo deste trabalho foi verificar em sangue de rato a compatibilidade entre a metodologia automatizada e a não automatizada. Foram realizadas contagens das variáveis hematológicas de 10 ratos Wistar, Rattus norvegicus. Para o método automatizado foram utilizados os aparelhos ABX-Pentra-60c+ e Celldyn-3200. Para o método não automatizado foi utilizado o hemocitômetro nas contagens de eritrócitos, de leucócitos e de plaquetas, utilizando o líquido de Hayem (1:200), o líquido de Türk (1:20) e o líquido de Brecher e Cronkite (1:200), respectivamente. A determinação do hematócrito foi obtida utilizando-se uma centrífuga de micro-hematócrito, a concentração de hemoglobina pelo método de cianometa-hemoglobina (líquido de Drabkin) e os índices hematimétricos absolutos calculados através dos resultados da série vermelha. A contagem diferencial de leucócitos foi realizada em extensões de sangue (panótico rápido). Os dados foram analisados através da variância ­ ANOVA, seguida pelo teste Tukey, com nível de significância de 5%. Os valores obtidos pelo hemocitômetro comparadas aos contadores hematológicos mostraram diferenças significativas (p < 0,05) para neutrófilos, eosinófilos e basófilos. Os resultados permitiram concluir que as contagens realizadas com os aparelhos utilizados foram confiáveis e rápidas, mas não são indicadas para a contagem diferencial de leucócito.


The aim of this study was to verify blood compatibility between automated and non-automated methodology in rats. Counts of haematological variables of 10 Wistar rat, Rattus norvegicus were made. For the automated method were used the ABX-Pentra-60c + equipment and Celldyn-3200. For non-automated method the hemocytometer was used to count the erythrocytes, leukocytes and platelets using the Hayem liquid (1: 200), the Türk liquid (1:20) and the Brecher and Cronkite liquid (1: 200), respectively. The determination of the hematocrit was obtained by using a microhematocrit centrifuge, hemoglobin concentration by cyanmethaemoglobin method (Drabkin liquid) and absolute RBC indices calculated by the results of red series. The differential leukocyte count was performed on blood extensions (fast Panotic). The data was analyzed through variance ­ ANOVA and then by Tukey test at 5% significance level. The values obtained by hemocytometer compared to hematological counters revealed significant differences (p < 0.05) for neutrophils, eosinophils and basophils. Through the results obtained, it was concluded that the counts made with the devices used were reliable and fast, but are not indicated for the differential count of white blood cell.


Assuntos
Animais , Plaquetas , Ratos Wistar , Leucócitos
15.
Artigo em Espanhol | LILACS | ID: biblio-844728

RESUMO

RESUMEN: Este caso clínico de boca dividida a 6 meses tiene como objetivo comparar los resultados obtenidos con una membrana de fibrina rica en plaquetas y leucocitos (L-PRF) y un injerto de tejido conectivo (ITC) en el tratamiento de recesiones gingivales clase1 de Miller en un paciente con biotipo gingival grueso. El resultado muestra que el uso de una membrana de L-PRF provee un parcial cubrimiento de la recesión y un menor malestar subjetivo del paciente cuando se compara con el ITC. Sin embargo, el ITC proporciona un mayor porcentaje de cubrimiento radicular y un mejor resultado estético que la membrana de L-PRF al cabo de 6 meses.


ABSTRACT: The aim of this split-mouth clinical study, covering a 6 month period, is to compare the results obtained from Leucocyte and Platelet Rich Fibrin Membrane (L-PRF) and Connective Tissue Grafting (CTG) in the treatment of class 1 Miller gingival recessions in a patient with thick gingival biotype. The results show that the use of L-PRF membrane provides partial coverage of the recession, as well as lower subjective patient discomfort when compared with CTG. However, CTG provides a higher percentage of root coverage and better aesthetic results than the L-PRF membrane after a period of 6 months.


Assuntos
Humanos , Feminino , Adulto , Plaquetas/fisiologia , Tecido Conjuntivo/transplante , Fibrina/uso terapêutico , Retração Gengival/cirurgia , Membranas Artificiais , Gengivoplastia
16.
Clinics ; 72(2): 116-124, Feb. 2017. graf
Artigo em Inglês | LILACS | ID: biblio-840046

RESUMO

OBJECTIVES: Previous reports have revealed that several cytokines (including platelet-derived growth factor-BB, transforming growth factors-β1 and insulin-like growth factor-1) can enhance the rate of bone formation and synthesis of extracellular matrix in orthopaedics or periodontology. This study aimed to determine the concentration of cytokines within platelet-rich fibrin microstructures and investigate whether there are differences in the different portions of platelet-rich fibrin, which has implications for proper clinical use of platelet-rich fibrin gel. METHODS: Whole blood was obtained from six New Zealand rabbits (male, 7 to 39 weeks old, weight 2.7-4 kg); it was then centrifuged for preparation of platelet-rich fibrin gels and harvest of plasma. The resultant platelet-rich fibrin gels were used for cytokine determination, histological analyses and scanning electron microscopy. All plasmas obtained were subject to the same cytokine determination assays for the purpose of comparison. RESULTS: Cytokines platelet-derived growth factor-BB and transforming growth factor-β1 formed concentration gradients from high at the red blood cell end of the platelet-rich fibrin gel (p=1.88×10-5) to low at the plasma end (p=0.19). Insulin-like growth factor-1 concentrations were similar at the red blood cell and plasma ends. The porosities of the platelet-rich fibrin samples taken in sequence from the red blood cell end to the plasma end were 6.5% ± 4.9%, 24.8% ± 7.5%, 30.3% ± 8.5%, 41.4% ± 12.3%, and 40.3% ± 11.7%, respectively, showing a gradual decrease in the compactness of the platelet-rich fibrin network. CONCLUSION: Cytokine concentrations are positively associated with platelet-rich fibrin microstructure and portion in a rabbit model. As platelet-rich fibrin is the main entity currently used in regenerative medicine, assessing cytokine concentration and the most valuable portion of PRF gels is essential and recommended to all physicians.


Assuntos
Animais , Masculino , Coelhos , Plaquetas/fisiologia , Citocinas/fisiologia , Plasma Rico em Plaquetas/fisiologia , Linhagem Celular , Centrifugação , Modelos Animais de Doenças , Géis/química , Plasma Rico em Plaquetas/química
17.
ImplantNewsPerio ; 2(1): 57-63, jan.-fev. 2017. tab
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-847077

RESUMO

Objetivo: realizar uma revisão da literatura em bases de dados eletrônicos sobre as evidências da eficácia da fibrina rica em plaquetas. Material e métodos: a partir dos bancos de dados Medline, PubMed e ClinicalKey, de março a junho de 2016, foi realizada um revisão integrativa de trabalhos sistemáticos e longitudinais. Dois revisores independentes avaliaram os critérios de inclusão e exclusão dos artigos, sendo incluídos artigos publicados de 2011 a maio de 2016. Resultados: a busca eletrônica resultou em um total de 16 referências. Conclusão: os estudos, em sua maioria, relataram aceleração do processo de cicatrização do tecido mole e diminuição da severidade das sequelas pós-operatórias imediatas em Odontologia.


Objective: to review the literature in electronic databases about the evidence of platelet rich fibrin efficiency. Material and methods: Medline, PubMed and ClinicalKey databases were searched from March to June 2016 using a combination of specific search terms. Two independent reviewers assessed the criteria for inclusion and exclusion of articles. with selected articles from 2011 to May 2016. Results: the electronic search resulted in a total of 16 references. Conclusion: most of the studies reported an acceleration of soft tissue healing process and severity decrease of the immediate postoperative sequelae in Dentistry.


Assuntos
Materiais Biocompatíveis , Plaquetas , Regeneração Óssea , Fibrina/uso terapêutico , Plasma Rico em Plaquetas
18.
Braz. j. med. biol. res ; 50(1): e5660, 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-839238

RESUMO

Clopidogrel and aspirin are the most commonly used medications worldwide for dual antiplatelet therapy after percutaneous coronary intervention. However, clopidogrel hyporesponsiveness related to gene polymorphisms is a concern. Populations with higher degrees of genetic admixture may have increased prevalence of clopidogrel hyporesponsiveness. To assess this, we genotyped CYP2C19, ABCB1, and PON1 in 187 patients who underwent percutaneous coronary intervention. Race was self-defined by patients. We also performed light transmission aggregometry with adenosine diphosphate (ADP) and arachidonic acid during dual antiplatelet therapy. We found a significant difference for presence of the CYP2C19*2 polymorphism between white and non-white patients. Although 7% of patients had platelet resistance to clopidogrel, this did not correlate with any of the tested genetic polymorphisms. We did not find platelet resistance to aspirin in this cohort. Multivariate analysis showed that patients with PON1 and CYP2C19 polymorphisms had higher light transmission after ADP aggregometry than patients with native alleles. There was no preponderance of any race in patients with higher light transmission aggregometry. In brief, PON1 and CYP2C19 polymorphisms were associated with lower clopidogrel responsiveness in this sample. Despite differences in CYP2C19 polymorphisms across white and non-white patients, genetic admixture by itself was not able to identify clopidogrel hyporesponsiveness.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Aspirina/farmacologia , Plaquetas/efeitos dos fármacos , Doença da Artéria Coronariana/tratamento farmacológico , Inibidores da Agregação de Plaquetas/farmacologia , Ticlopidina/análogos & derivados , Alelos , Arildialquilfosfatase/genética , Subfamília B de Transportador de Cassetes de Ligação de ATP/genética , Doença da Artéria Coronariana/genética , Citocromo P-450 CYP2C19/genética , Quimioterapia Combinada , Genótipo , Intervenção Coronária Percutânea , Polimorfismo Genético , Estudos Prospectivos , Ticlopidina/farmacologia
19.
Braz. oral res. (Online) ; 31: e15, 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-839515

RESUMO

Abstract Anorganic bovine bone mineral (ABBM) is extensively used in the treatment of intra-bony defects. Platelet-rich fibrin (PRF) is a new-generation platelet concentrate with a simplified technique. Although certain studies have reported the use of PRF in the treatment of intra-bony defects, to date, none of them have evaluated its additive effects with ABBM. Therefore, a randomised, split-mouth clinical trial was conducted to compare healing of intra-bony defects treated with an ABBM-PRF combination with healing of those treated with ABBM alone. By using a split-mouth design, 15 paired intra-bony defects were randomly treated with either ABBM alone (control group) or ABBM-PRF combination (test group). Following clinical parameters and radiographical measurements were recorded at baseline and 6 months after treatment: plaque index (PI), gingival index (GI), probing depth (PD), gingival recession (GR), clinical attachment level (CAL), vertical bone loss, depth of defect and defect angle. Preoperative clinical and radiographical measurements were similar for the test and control groups. Statistically significant reductions in GI, PD, CAL, vertical bone loss, depth of intra-bony defect and widening of defect angle were detected after treatment in both groups. With respect to inter-group analysis, gain in CAL was significantly greater in the test group than in the control group, whereas no inter-group differences were observed in any other parameter. The results of this study indicate that both therapies are effective in the treatment of intra-bony defects.


Assuntos
Humanos , Animais , Masculino , Feminino , Adulto , Bovinos , Cicatrização/efeitos dos fármacos , Plaquetas , Regeneração Óssea/efeitos dos fármacos , Fibrina/uso terapêutico , Perda do Osso Alveolar/terapia , Substitutos Ósseos/uso terapêutico , Fatores de Tempo , Índice Periodontal , Índice de Placa Dentária , Reprodutibilidade dos Testes , Perda do Osso Alveolar/diagnóstico por imagem , Resultado do Tratamento , Terapia Combinada , Estatísticas não Paramétricas , Retração Gengival
20.
Braz. J. Pharm. Sci. (Online) ; 53(1): e15195, 2017. tab
Artigo em Inglês | LILACS | ID: biblio-839458

RESUMO

Abstract In recent years, several studies have described the clinical impact of bacterial infection associated with transfusion of platelet concentrates (PCs). Among the blood components, PCs are responsible for the highest rates of bacterial contamination as well as septic transfusion reactions. We assessed antimicrobial susceptibility profile, resistance to methicillin (MRCoNS), and resistance to macrolides, lincosamides and streptogramins of group B (MLSB) of 16 coagulase-negative staphylococci (CoNS) isolates from an investigation in 691 PCs bags. We then compared conventional and automated phenotypic methods, disc diffusion test (DD) and VITEK(r) 2, respectively as well as phenotypic and genotypic methods (Polymerase Chain Reaction - PCR). All CoNS were susceptible to vancomycin. The disc diffusion test characterized 18.75% as MRCoNS and 37.5% with inducible resistance to MLSB (iMLSB), and with VITEK(r) 2, 6.3% and 31.25%, respectively. The mecA gene was detected in 18.75% and the erm gene in 31.25% of the isolates. In this study, we found equal percentage values between presence of the mecA gene by PCR and resistance to methicillin using cefoxitin by DD test, evidence of the erm gene by PCR, and iMLSB resistance by automation (VITEK(r) 2). Moreover, we identified three strains with beta-lactamase overproduction, and the occurrence of a bigger mistake was verified when automation was compared with DD test. And we observed that D-test was the most reliable for the detection of iMLSB resistance in Staphylococcus sp.


Assuntos
Plaquetas/classificação , Suscetibilidade a Doenças/metabolismo , Genes/efeitos dos fármacos , Staphylococcus/classificação , Coagulase/análise
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