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Biocell ; 34(2): 91-94, Aug. 2010. ilus, graf
Artigo em Inglês | LILACS | ID: lil-595038


CXCL-12 and its receptor CXCR4 participate in breast cancer and melanoma cell metastasis to bone and lymphoid nodes. CD44, as a receptor for hyaluronic acid, is involved in lymphocyte recirculation, homing, adhesion and migration. But the role of CD44 in CXCL-12 induced leukemia cell migration still remains unclear. The present study showed that CXCL-12 stimulation induced the rapid internalization of CXCR4 and facilitated the formation of lamellipodia and uropod in acute leukemia cell line HL-60. CXCL-12 also induced CD44 translocation into the uropod, while CD44 remained evenly distributed on the untreated cell membranes. Results suggest that CD44 participates in CXCL-12 induced cell polarization and subsequent cell migration.

Humanos , /imunologia , Extensões da Superfície Celular/metabolismo , Leucemia Mieloide/imunologia , Movimento Celular/fisiologia , Polaridade Celular , Ácido Hialurônico , Quimiocinas/imunologia
Biocell ; 27(2): 213-224, Aug. 2003.
Artigo em Inglês | LILACS | ID: lil-384241


CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran-5,6-dione; CG-NQ), a beta-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 microM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen peroxide. Expression of such effects varied according to hepatocyte samples and taken together strongly support an apoptotic action of CG-NQ dependent on reactive oxygen species.

Humanos , Masculino , Apoptose/efeitos dos fármacos , Hepatócitos/efeitos dos fármacos , Naftoquinonas/farmacologia , Naftoquinonas/toxicidade , Apoptose/fisiologia , Células Cultivadas , Cromatina/efeitos dos fármacos , Cromatina/patologia , Extensões da Superfície Celular/efeitos dos fármacos , Extensões da Superfície Celular/patologia , Extensões da Superfície Celular/ultraestrutura , Fragmentação do DNA/efeitos dos fármacos , Fragmentação do DNA/fisiologia , Hepatócitos/metabolismo , Hepatócitos/ultraestrutura , Microscopia Eletrônica , Membranas Intracelulares/efeitos dos fármacos , Membranas Intracelulares/patologia , Membranas Intracelulares/ultraestrutura , Microvilosidades/efeitos dos fármacos , Microvilosidades/patologia , Microvilosidades/ultraestrutura , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/patologia , Mitocôndrias/ultraestrutura , Peróxido de Hidrogênio/metabolismo , Ratos , Ratos Wistar , Retículo Endoplasmático/efeitos dos fármacos , Retículo Endoplasmático/patologia , Retículo Endoplasmático/ultraestrutura
Rev. argent. microbiol ; 33(3): 155-159, jul.-sept. 2001.
Artigo em Inglês | LILACS | ID: lil-332486


Our original aim was to determine whether dBcAMP-induced activation of cultured astrocytes affected the course of subsequent viral infection. After 2 h exposure of 2-day-old first subculture of mouse astrocytes to dBcAMP 1 mM, cell monolayers grown in glass coverslips of Leighton tubes were inoculated with 10(3) PFU of Theiler virus-GDVII strain (TMEV-GDVII). At 9 days post-infection (pi), viral infectivity persisted in supernatants from dBcAMP-treated cultures, but was no longer detectable in non-stimulated controls. The relatively spared astroglial monolayer at day 1 pi, hardly affected by progressive viral cytolytic effect, was chosen for immunolabeled cell count, whether by viral antigen or GFAP. To this end, 20 fields for each coverslip were digitalized at 250x final magnification. In dBcAMP treated cultures, viral antigen(+) cells were fewer and lower in percentage versus infected cultures lacking stimulation. As regards GFAP staining, stimulation or infection per se induced a greater number and percentage of labeled astrocytes. According to morphometric characterization, such increase was due to a greater number of process-bearing astrocytes. It may be concluded that, regardless of previous dBcAMP treatment, early TMEV-GDVII infection enhanced immunocytochemical and morphological differentiation in cultured astrocytes.

Animais , Camundongos , Astrócitos , Theilovirus , Antígenos Virais/análise , Astrócitos , Bucladesina , Tamanho Celular , Células Cultivadas/efeitos dos fármacos , Cérebro , Efeito Citopatogênico Viral , Diferenciação Celular/efeitos dos fármacos , Extensões da Superfície Celular/ultraestrutura , Processamento de Imagem Assistida por Computador , Biomarcadores , Camundongos Endogâmicos BALB C , Proteína Glial Fibrilar Ácida/análise , Theilovirus