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1.
Acta cir. bras ; 34(10): e201901005, Oct. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1054671

RESUMO

Abstract Purpose: To quantify and compare the expression of stromal elements in prostate adenocarcinoma of different Gleason scores with non-tumor area (control). Methods: We obtained 132 specimens from samples of prostate peripheral and transition zone. We analyzed the following elements of the extracellular matrix: collagen fibers, elastic system, smooth muscle fibers and blood vessels. The tumor area and non-tumor area (control) of the TMA (tissue microarray) were photographed and analyzed using the ImageJ software. Results: The comparison between the tumor area and the non-tumor area showed significant differences between stromal prostate elements. There was an increase of collagen fibers in the tumor area, mainly in Gleason 7. Elastic system fibers showed similar result, also from the Gleason 7. Blood vessels showed a significant increase occurred in all analyzed groups. The muscle fibers exhibited a different behavior, with a decrease in relation to the tumor area. Conclusions: There is a significant difference between the extracellular matrix in prostate cancer compared to the non-tumor area (control) especially in Gleason 7. Important modifications of the prostatic stromal elements strongly correlate with different Gleason scores and can contribute to predict the pathological staging of prostate cancer.


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Próstata/patologia , Adenocarcinoma/patologia , Células Estromais/patologia , Valores de Referência , Vasos Sanguíneos/patologia , Estudos Retrospectivos , Colágeno/análise , Análise Serial de Tecidos , Tecido Elástico/anatomia & histologia , Gradação de Tumores , Músculo Liso/patologia
2.
Appl. cancer res ; 37: 1-6, 2017. ilus
Artigo em Inglês | LILACS, Inca | ID: biblio-911499

RESUMO

Background: Gastrointestinal stromal tumors (GISTs) originate as precursor cells of the interstitial cells of Cajal in the myenteric plexus and generally have a mutation in the tyrosine kinase receptor, C-KIT (CD117). The objective is to evaluate the clinical, epidemiological, and therapeutic profiles of GIST cases available from a hospital specializing in cancer treatment. Methods: A retrospective, longitudinal study of 85 GIST cases in a Cancer Center (São Paulo, Brazil) was conducted. Results: The cases identified involved 40 men and 45 women and the average age at diagnosis was 55.7 ± 14. 8 years (median, 57). The symptoms present at diagnosis depended on the location and dimensions of each lesion. In 49 cases (57.6%), the tumors had a gastric location and the mean dimensions were 7.2 ± 2.3 cm (median, 3.4 cm). Recurrent metastatic disease presented in 27 cases (with the liver mainly affected). Locoregional recurrence was detected in 16 cases. C-KIT was positive in 79/81 (97.5%) of the cases examined. Most of the tumors were initially treated with surgery, while clinical treatment was applied to the recurrent cases. The overall survival rate was 76.4% 162 months after diagnosis. Conclusions: The GISTs examined most commonly originated in the stomach, while the liver was the main site affected by metastatic lesions. Most of the lesions appeared to be slow-growing neoplasms that were positive for C-KIT (CD117). Complications, as well as death, mostly affected the elderly patients that had comorbidities or more aggressive forms of the disease (AU)


Assuntos
Humanos , Células Estromais , Receptores Proteína Tirosina Quinases , Tumores do Estroma Gastrointestinal/terapia
3.
Acta cir. bras ; 31(9): 578-585, Sept. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-795994

RESUMO

ABSTRACT PURPOSE: To analyze the healing effects of stromal vascular fraction (SVF) application compared to wound dressing with 2% silver sulfadiazine in full thickness burn wounds in rats. METHODS: Animals were divided into two groups: 2% silver sulfadiazine group and SVF group. Both groups received occlusive bandages while the first one was treated with 2% silver sulfadiazine and the latter was treated with injections of SVF prepared from adipose tissue extracted from an animal donor. The animals were accompanied through 3, 7 and 30 days for evaluation of macroscopic, microscopic and morphometric aspects. RESULTS: On day three, a significant increase (p<0.05) of infiltration of polymorphonuclear, fibrin formation and fibroblasts migration in SVF group was observed. On the 7th day the mononuclear infiltrate, angiogenesis, collagen and fibroblasts were significantly increased in the SVF group (p<0.05). At 30 days significantly increased collagen deposition was observed in the SVF group (p<0.05) . CONCLUSION: Adipose tissue derived stromal vascular fraction injections promotes better wound repair than 2% silver sulfadiazine in the treatment of full thickness burn in rats during the evaluated experimental period.


Assuntos
Animais , Masculino , Sulfadiazina de Prata/administração & dosagem , Cicatrização , Queimaduras/terapia , Tecido Adiposo/transplante , Anti-Infecciosos Locais/administração & dosagem , Bandagens , Cicatrização/efeitos dos fármacos , Queimaduras/cirurgia , Queimaduras/patologia , Tecido Adiposo/citologia , Células Estromais/citologia , Células Estromais/transplante , Ratos Wistar , Modelos Animais de Doenças , Microscopia
4.
Rev. chil. cir ; 68(4): 295-301, jul. 2016. graf, tab
Artigo em Espanhol | LILACS | ID: lil-788897

RESUMO

Objetivo Obtener células estromales derivadas del tejido adiposo, medir y comparar las tasas de viabilidad antes e inmediatamente después un ciclo de criopreservación con diferentes combinaciones de criopreservantes de manera de obtener el mejor medio de criopreservación. Material y método Medición de la tasa de viabilidad poscriopreservación de células estromales derivadas del tejido adiposo obtenidas de 5 pacientes utilizando medios definidos (DMEM/Ham F12) libres de suero bovino y suplementados con una de los siguientes combinaciones de compuestos: dimetilsulfóxido (DMSO) 10%; DMSO 10% + trehalosa 7,6%; DMSO 10% + albúmina humana 10% y DMSO 10% + trehalosa 7,6% + albúmina humana 10%, mediante citometría de flujo con ioduro de propidio. Resultados No existen diferencias estadísticamente significativas en las tasas de viabilidad de las células estromales posterior a un ciclo de criopreservación. Sin embargo, se observa una tendencia a mejorar la tasa de recuperación de células vitales al agregar albúmina humana. Conclusiones No se observaron diferencias significativas entre las condiciones estudiadas, sugiriendo que ninguna es superior a las demás en cuanto a rendimiento. Es así como podemos afirmar que la criopreservación de las células estromales derivadas del tejido adiposo en un medio que combine DMEM/F12 con DMSO 10% + trehalosa 7,6% + albúmina humana 10% no logra una tasa de recuperación de células vitales significativamente mayor que las congeladas solo con DMSO 10%.


Aim To obtain stromal cells derived from adipose tissue, to measure and compare viability rates before and immediately after cryopreservation cycle, using different combinations of cryoprotective agents in order to identify the best cryopreservation medium. Material and method Viability rate after cryopreservation of stromal cells derived from adipose tissue were assessed by flow cytometry with propidium iodide. Samples of stromal cells obtained from 5 patients were kept defined, bovine serum-free media (DMEM/Ham-F12), supplemented with one of the following combinations of compounds: 10% dymethylsulfoxide (DMSO); Trehalose 10% DMSO + 7.6%; 10% DMSO + 10% human albumin and 10% DMSO + 7.6% Trehalose + 10% human albumin. Results No statistically significant differences were observed in the viability rates of stromal cells derived from adipose tissue after a cryopreservation cycle. However, we observed a tendency towards improvement of recovery rate when human albumin was added to the medium. Conclusions None of the studied conditions proved superior to others in terms of cell vitality after a cryopreservation cycle. Hence, we conclude that the cryopreservation of stromal cells derived from adipose tissue in an environment that combines DMEM/F12 with 10% DMSO + 7.6% Trehalose + human albumin 10% does not achieve a significantly higher recovery rate than only frozen solely with DMSO 10%.


Assuntos
Humanos , Criopreservação/métodos , Sobrevivência Celular/efeitos dos fármacos , Células Estromais/fisiologia , Crioprotetores/farmacologia , Trealose/farmacologia , Dimetil Sulfóxido/farmacologia , Tecido Adiposo/citologia , Albumina Sérica Humana/farmacologia , Congelamento
5.
ARS med. (Santiago, En línea) ; 41(1): 5-13, 2016. ilus, Graf, Tab
Artigo em Espanhol | LILACS | ID: biblio-1015462

RESUMO

La regeneración de tejidos usando células, factores de crecimiento y soportes constituyen una alternativa en la Medicina Regenerativa. La fibroína de seda es un excelente biosoporte, sus propiedades mecánicas únicas le permiten soportar procesos de adhesión y crecimiento celular. Objetivo. Evaluar la fibroína de la seda obtenida del gusano de seda Bombyx mori L como material de soporte para el crecimiento de células mesenquimales estromales de pulpa dental (CMPD). Métodos. La fibroína obtenida a partir de capullos de gusanos de seda Bombyx mori L criados en la Granja El Pílamo, propiedad de la Universidad Tecnológica de Pereira, se empleó para la fabricación de películas de fibroína íntegras y resistentes a condiciones de cultivo. Las CMPD fueron obtenidas a partir de un donante de diente premolar, la pieza dental se cortó con disco de diamante para la obtención de la pulpa que fue sometida a disgregación enzimática. Las células obtenidas se subcultivaron hasta el segundo pase, para posteriormente transferirse a cajas de cultivo que contenían películas de fibroína, se sometieron a condiciones inherentes al proceso de incubación siguiendo su crecimiento y viabilidad celular durante 27 días. Resultados. Al final del periodo de incubación, se observaron películas integras, estables y resistentes que permitieron el crecimiento celular. Conclusión. Se plantea el uso de fibroína como un biopolímero natural que brinda un soporte mecánico, un microambiente óptimo y un mimetismo de la estructura organizacional de los tejidos, postulándose como un potencial biomaterial para procesos de crecimiento celular en Medicina Regenerativa e Ingeniería de Tejidos.(AU)


Tissue regeneration using cells, growth factors and supports are an alternative in Regenerative Medicine. Silk fibroin is an excellent biosupport, its unique mechanical properties allow it to support processes of cell adhesion and growth. Objective: Evaluating the obtained silk´s fibroin from silkworm (Bombyx mori L) as a scaffold material for growth of dental pulp mesenchymal stromal cells (CMPD). Methods: The fibroin was obtained from silkworm (Bombyx mori L) cocoons reared at "The Pilamo" Farm, owned by the Universidad Tecnológica de Pereira. Procedures for obtaining full and resistant fibroin films to culture conditions were performed. The CMPD were obtained from a premolar tooth, the tooth was cut with a diamond blade to obtain the pulp which was subjected to enzymatic digestion. The cells obtained were subcultured until the second pass, and then, transferred to culture dishes containing fibroin films. This cells were cultured in standard conditions and tracking incubation with cell viability and growth for 27 days. Results: At the end of the incubation period, we realize that the fibroin films were intact and also this fibroin films allows cell growth.Conclusion: The use of fibroin as a natural biopolymer provides mechanical support, also an optimal microenvironment and mimic the organizational structure of tissues, so we postulated fibroin as a potential biomaterial for cell growth to be used in regenerative medicine and tissue engineering.(AU)


Assuntos
Humanos , Masculino , Feminino , Materiais Biocompatíveis , Fibroínas , Células Estromais , Engenharia Tecidual , Polpa Dentária , Medicina Regenerativa
6.
Int. braz. j. urol ; 41(5): 849-858, Sept.-Oct. 2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-767051

RESUMO

ABSTRACT Introduction and Objectives: Reactive Stroma (RStr) is observed in many human cancers and is related to carcinogenesis. The objectives of the present study were to stablish a relationship of the RStr microenvironment with prostate cancer (Pca) through a morphological and molecular characterization, and to identify a possible relationship between RStr with worse prognosis factors and occurrence of malignant prostatic stem cells. Materials and Methods: Forty prostatic samples were selected from men with Pca diagnosis submitted to radical prostatectomy; they were divided in two groups: Group-1 (n=20): samples without reactive stroma; Group-2 (n=20): samples of PCa with intense stroma reaction. Prostatic samples were evaluated for RStr intensity by Masson Trichromic stain and posteriorly submitted to histopathological and immunohistochemistry analysis for antigens: α-actin, vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA, AR, Erα and ERβ. Results: Reactive stroma with intense desmoplastic reactivity was significantly more frequent in intermediate (Gleason 7, 3+4) and high grade tumors (Gleason 7, 4+3). The group with intense stromal reactivity showed significant higher levels of Vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA and ERα. Conclusions: It can be concluded that RStr may be a predictive marker of Pca progression, since it was associated with increase of growth factors, imbalance of androgen and estrogen receptors and presence of malign prostatic stem cells.


Assuntos
Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Adenocarcinoma/patologia , Células Epiteliais/patologia , Células-Tronco Neoplásicas/patologia , Neoplasias da Próstata/patologia , Células Estromais/patologia , Actinas/análise , Adenocarcinoma/química , Antígenos de Neoplasias/análise , Biomarcadores Tumorais/análise , Progressão da Doença , Proteínas de Ligação a DNA/análise , Células Epiteliais/química , Receptor alfa de Estrogênio/análise , /análise , Proteínas Ligadas por GPI/análise , Imuno-Histoquímica , Fator de Crescimento Insulin-Like I/análise , /análise , Gradação de Tumores , Proteínas de Neoplasias/análise , Células-Tronco Neoplásicas/química , Neoplasias da Próstata/química , Células Estromais/química , Microambiente Tumoral , Fatores de Transcrição/análise , Vimentina/análise
7.
Braz. j. med. biol. res ; 48(6): 557-567, 06/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-748226

RESUMO

Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral stroma. Similarly significant was the direct association between N1 tumors and high levels of HA in cancer cells. Cox multivariate analysis showed significant association between better survival and low HA. HA increased in sputum from lung cancer patients compared to cancer-free and healthy volunteers and a significant correlation was found between HA in sputum and HA in cancer tissue. Localization of HA in tumor tissue was related to malignancy and reflected in sputum, making this an emerging factor for an important diagnostic procedure in patients suspected to have lung cancer. Further study in additional patients in a randomized prospective trial is required to finalize these results and to validate our quantitative assessment of HA, as well as to couple it to gold standard sputum cytology.


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma/química , Ácido Hialurônico/análise , Neoplasias Pulmonares/química , Escarro/química , Biópsia , Biomarcadores Tumorais/análise , Estudos de Casos e Controles , Carcinoma/patologia , Ensaio de Imunoadsorção Enzimática , Imuno-Histoquímica , Neoplasias Pulmonares/patologia , Pulmão/química , Pulmão/patologia , Estadiamento de Neoplasias , Reprodutibilidade dos Testes , Fatores de Risco , Sensibilidade e Especificidade , Estatísticas não Paramétricas , Fumar/efeitos adversos , Células Estromais/química , Células Estromais/patologia
8.
Einstein (Säo Paulo) ; 13(2): 276-278, Apr-Jun/2015. graf
Artigo em Inglês | LILACS | ID: lil-751428

RESUMO

Female patient, 42-years-old, complaining of difficulty in urinating and swelling in the vulvar area for one year. Her gynecological examination showed extensive injury in the vulvar region and the biopsy done was inconclusive. The removal of the lesion was conducted. After the procedure, the patient remains free of recurrence for 15 months. This case highlights the need to consider angiomyxoma in the differential diagnosis for tumors of unknown cause in the vulvar region.


Paciente do gênero feminino, 42 anos, com queixas de dificuldade ao urinar e aumento de volume na região vulvar há 1 ano. Ao exame ginecológico, apresentava extensa lesão na região vulvar. Biópsia da lesão foi inconclusiva. Realizou-se a exérese da lesão. A paciente permanece livre de recorrências há 15 meses. Este caso destaca a necessidade de considerar o angiomixoma no diagnóstico diferencial de massas de causa desconhecida na região vulvar.


Assuntos
Adulto , Feminino , Humanos , Mixoma/patologia , Vulva/patologia , Neoplasias Vulvares/patologia , Biópsia , Diagnóstico Diferencial , Mixoma/cirurgia , Células Estromais/patologia , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Neoplasias Vulvares/cirurgia
9.
Int. j. morphol ; 33(1): 337-341, Mar. 2015. ilus
Artigo em Inglês | LILACS | ID: lil-743807

RESUMO

Endometriosis is an estrogen-dependent disease in reproductive age women. Adiponectin and Nitric oxide (NO) have an important role in physiologic functions especially in human reproductive system. Levels of NO increased in the endometriosis patients but serum adiponectin levels decreased in woman with endometriosis. The aim of this study was to determine adiponectin effect on nitric oxide secretion by cultured normal and endometriotic human endometrial stromal cells. In this experimental study, normal (n= 10) and endometriotic endometrial biopsies (n= 10) were taken in sterile condition. Stromal cells isolated and cultured in in DMEM/ F12 medium and treated with adiponectin concentrations (0, 10, 100, and 200 ng/ml) for 24 and 48 hours. NO assay was done on their supernatants by Greiss method. Data was analyzed by one way ANOVA and p<0.05 was considered significant. There was significant difference between endometriosis groups in NO secretion in all dose of adiponectin and time (p<0.05). In normal groups there was significant difference in 48 hours (p<0.05) but no significant change in 24 hours (p>0.05). Adiponectin effects nitric oxide secretion of cultured human endometriotic stromal cells.


La endometriosis es una enfermedad dependiente de estrógenos que se presenta en mujeres en edad reproductiva. La adiponectina y el óxido nítrico (ON) tienen un papel importante en las funciones fisiológicas, especialmente en el sistema reproductivo humano. Los niveles de ON aumentan en los pacientes con endometriosis, pero los niveles de adiponectina en suero disminuyen. El objetivo fue determinar el efecto de la adiponectina sobre la secreción de ON por las células estromales de endometrio humano, tanto normales como con endometriosis, en medio de cultivo. En este estudio experimental, las células estromales de endometrio normales (n= 10) y las biopsias de endometrio con endometriosis (n= 10) se tomaron en condiciones de esterilidad. Las células estromales fueron aisladas y cultivadas en un medio DMEM/F12, y se sometieron a distintas concentraciones de adiponectina (0, 10, 100, y 200 ng/ml) durante 24 y 48 horas. El ensayo con ON se realizó a los sobrenadantes obtenidos por el método de Greiss. Los datos recolectados fueron analizados por ANOVA de una vía y un valor p<0,05 se consideró significativo. Entre los grupos con endometriosis, en referencia a la secreción de ON, no hubo diferencia significativa en todas las dosis de adiponectina y los tiempos estipulados (p<0,05). En los grupos normales, hubo diferencia significativa a las 48 horas (p<0,05), pero ningún cambio significativo a las 24 horas (p>0,05). La adiponectina tiene efectos sobre la secreción de óxido nítrico por las células estromales endometriales humanas en cultivo.


Assuntos
Humanos , Feminino , Endometriose/metabolismo , Endometriose/patologia , Adiponectina/metabolismo , Células Cultivadas , Células Estromais/metabolismo , Endométrio/metabolismo , Endométrio/patologia , Óxido Nítrico/metabolismo
10.
Rev. chil. urol ; 80(2): 72-74, 2015. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-786487

RESUMO

Los tumores estromales de potencial maligno incierto (STUMP) se enmarcan dentro de las lesiones de células fusadas de la próstata, entidades de relativa infrecuencia en la práctica urológica habitual, contabilizándose entre 0,1-0,2 por ciento 1 de todas los tumores malignos prostáticos. Corresponden a una proliferación no epitelial mesenquimática de células fusadas estromales, que pueden adquirir la capacidad de infiltrar el epitelio glandular prostático. Representan un desafío tanto para el patólogo, por la dificultad diagnóstica, como para el clínico, pues no existe consenso respecto a su manejo. Materiales y métodos: estudio retrospectivo descriptivo. Se filtró la base de datos del servicio de Anatomía Patología del Hospital San Borja Arriarán con los términos clave “STUMP”, “Tumor estromal próstata”, tanto de biopsias transrrectales como de piezas operatorias. De los pacientes con diagnóstico histológico e inmunohistoquímico compatible, se registraron características epidemiológicas, estudio, manejo y sobrevida global. Resultados: se encontraron 3(tres) pacientes con diagnóstico histológico de STUMP. Edad promedio al diagnóstico 66años. Dos de los casos (66,6 ´po0r ciento) correspondieron a hallazgo en biopsia diferida por cirugía benigna prostática, (una por Adenomectomía transvesical y otra por RTU-P). El tercer caso correspondió a un hallazgo en biopsia por punción transrrectal ecodirigida, en contexto de PSA elevado (100ng/mL) y 4 biopsias previas: 3 normales, y una con informe de posible sarcoma prostático. La terapia de elección en 2 casos fue seguimiento y en uno se indicó cirugía radical. En los casos descritos no se registra mortalidad, con seguimiento promedio de 32 meses. Conclusión: Los tumores estromales de potencial maligno incierto son un diagnóstico histológico infrecuente, descrito como hallazgo tanto en la biopsia transrrectal como de pieza quirúrgica. No existen consensos internacionales ni guías de manejo. En nuestra experiencia...


Stromal tumors of uncertain malignant potential (STUMP) are included in the spindle cell lesions of the adult prostate, a very rare diagnostic entity in the clinical practice, accounting no more than 0,1-0,2 percent of all prostate malignancies. STUMP is defined as a non epithelial proliferation of spindle cells, which can acquire the ability of infiltrate the prostate glands. This lesions represent a challenge for both clinical and pathology physician. Material and methods: retrospective cohort study. A research was performed at the San Borja Hospital Pathology Service’s database, using keywords “STUMP”, “Stromal prostate tumor”. From eligible patients we took epidemiology and clinical data. Results: a total of three patients were included. Average age was 66 years. STUMP was founded in two patients in the pathology report after benign prostatic surgery (Simple prostatectomy and TUR-P). A third patient was diagnosed by a needle biopsy (TRUS Biopsy). This patient had an elevated PSA (>100 ng/mL), 4 previous biopsies, three normal and a fourth suspicious of prostate sarcoma. Surveillance was the therapy chosen for those patients diagnosed with STUMP after benign surgery. Radical prostatectomy was offered to the third patient. Average follow up was 32 month Conclusion: STUMP is a rare diagnostic, and is a challenge for both clinical and pathology physician. There are no guidelines for the management. In our series we confirmed the low frequency of this condition, and the management in our service is similar as other series described in literature...


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Células Estromais/patologia , Neoplasias da Próstata/cirurgia , Neoplasias da Próstata/epidemiologia , Epidemiologia Descritiva , Neoplasias da Próstata/patologia , Estudos Retrospectivos
11.
J. bras. nefrol ; 36(4): 490-495, Oct-Dec/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-731157

RESUMO

Introduction: Patients with chronic kidney disease present selenium (Se) plasma deficiency which is an essential trace element with important biological functions and, the best known biological role is attributed to its presence in the antioxidant enzyme, glutathione peroxidase (GPx). The Se content of foods depends on soil and some authors have suggested that Amazon soil (North Brazilian region) has high Se concentrations when compared to other regions of Brazil. Objective: The objective of this work was to compare the Se status in hemodialysis (HD) patients from North and Southeast of Brazil. Methods: Thirty-eight patients from Southeast region (22 men and 16 women, 15% diabetic, 53.5 ± 26.4 yrs) were compared to 40 patients from North region (28 men and 12 women, 22.5% diabetic, 63.5 ± 11.9 yrs). Se in plasma was determined through atomic absorption spectrophotometry with hydride generation. Results: The plasma Se levels in patients from Southeast region were significantly lower (17.5 ± 11.9 μg/L) when compared to patients from the North (37.1 ± 15.8 μg/L) (p < 0.001). However, both patient groups presented low Se plasma levels when compared to recommended values (60- 120 μg/L). There was no correlation between plasma Se levels and analyzed parameters. Conclusion: We concluded that patients from North (Amazon) region present higher plasma Se levels when compared to the patients from Southeast of Brazil. However, independently of the region, HD patients presented Se deficiency. .


Introdução: Pacientes com Doença Renal Crônica apresentam deficiência de selênio (Se), um elemento essencial, com importantes funções biológicas, como a de ser componente da enzima antioxidante glutationa peroxidase (GPx). A concentração de Se nos alimentos depende de sua concentração no solo e autores relatam que o solo da Amazônia possui elevados níveis de Se. Objetivo: O objetivo do trabalho foi comparar o estado nutricional do Se em pacientes em hemodiálise (HD) das regiões Norte e Sudeste do Brasil. Métodos: Trinta e oito pacientes da região Sudeste (22 homens e 16 mulheres, 15% diabéticos, 53,5 ± 26,4 anos) foram comparados com 40 pacientes da região Norte (28 homens e 12 mulheres, 22,5% diabéticos, 63,5 ± 11,9 anos). O Se no plasma foi determinado por espectrofotometria de absorção atômica por geração de hidretos acoplados a cela de quartzo. Resultados: Os níveis de Se dos pacientes em HD da região Sudeste foram significativamente menores (17,5 ± 11,9 μg/L) comparados aos pacientes da região Norte (37,1 ± 15,8 μg/L) (p < 0,001). Entretanto, ambos os grupos apresentaram níveis de Se abaixo da recomendação (60-120 μg/L). Não houve associação entre os níveis de Se e os parâmetros analisados. Conclusão: Com base nos resultados, concluímos que os pacientes da região Norte apresentaram elevados níveis de Se quando comparados com os pacientes da região Sudeste do Brasil. Entretanto, independentemente da região, ambos os grupos apresentaram deficiência com relação ao estado nutricional do Se. .


Assuntos
Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Neoplasias da Mama/metabolismo , Neoplasias da Mama/mortalidade , Timidina Fosforilase/metabolismo , Administração Oral , Antimetabólitos Antineoplásicos/administração & dosagem , Antimetabólitos Antineoplásicos/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Neoplasias da Mama/cirurgia , Intervalo Livre de Doença , Esquema de Medicação , Floxuridina/administração & dosagem , Floxuridina/uso terapêutico , Japão , Valor Preditivo dos Testes , Prognóstico , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Retrospectivos , Análise de Sobrevida , Células Estromais/metabolismo , Células Tumorais Cultivadas/metabolismo
12.
Acta cir. bras ; 29(9): 596-602, 09/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-722126

RESUMO

PURPOSE: To assess the evolution profile of the immunohistochemical expression of stromal constituents over the time-course of wound healing in a murine model. METHODS: Surgical wounds were performed in the back of 24 Wistar rats. After three, seven, 14 and 21 days, six rats were euthanized and the wounded histologically processed to assess the immunohistochemical expression of CD3, CD20, CD31, α-SMA and type-I collagen. Non-injured skin samples (NSS) were used as control. Data were subjected to statistical analysis using ANOVA and Tukey test. RESULTS: The mean of CD3 and CD20 positive cells in the wounds was significantly higher than in NSS at seven and 14 days (p<0.001). The blood vessels content was significantly lower than in NSS (p<0.05) at three days, but increased at seven and 14 days (p<0.01). The mean of α-SMA positive cells at seven, 14 and 21 days was higher than in NSS (p<0.05). The relative content of type I collagen increased from three to 21 days, but remained lower than in NSS (p<0.05). CONCLUSIONS: Lymphoid cells, myofibroblasts and microvessels contents varied over the time-course of wound healing, with peak at seven days and progressive reduction until 21 days. The type I collagen content increased over time. .


Assuntos
Animais , Masculino , Actinas/metabolismo , Antígenos CD/metabolismo , Colágeno Tipo I/metabolismo , Modelos Animais de Doenças , Linfócitos/patologia , Pele/lesões , Cicatrização/fisiologia , /metabolismo , /metabolismo , /metabolismo , Imuno-Histoquímica , Miofibroblastos/patologia , Ratos Wistar , Pele/metabolismo , Células Estromais/metabolismo , Células Estromais/patologia , Fatores de Tempo
13.
Rev. chil. obstet. ginecol ; 79(3): 187-192, jun. 2014. ilus
Artigo em Espanhol | LILACS | ID: lil-720213

RESUMO

La hiperplasia pseudoangiomatosa (PASH) es una lesión proliferativa benigna de la mama, poco frecuente, caracterizada por la existencia de lagos pseudovasculares embebidos en una gran proliferación del estroma mamario. Probablemente, el desarrollo de la PASH tenga una influencia hormonal, por lo que típicamente se diagnostica en mujeres en edad fértil. La PASH es un hallazgo histopatológico casual en las piezas quirúrgicas y biopsias mamarias realizadas por otra patología. La presentación clínica en forma de masa palpable es poco frecuente. El principal diagnóstico diferencial debe realizarse con el angiosarcoma de bajo grado. El tratamiento de la PASH nodular es una correcta exéresis quirúrgica asegurando borde sano amplio. El pronóstico es excelente, con un mínimo riesgo de recidiva si se realiza una adecuada cirugía. Se presenta el caso de una mujer de 37 años que acude a consulta por un nódulo mamario de crecimiento rápido.


Pseudoangiomatous stromal hyperplasia (PASH) of the breast is a rare benign proliferative mesenchymal lesion characterized by the presence of open slit like spaces embedded in a hyalinized fibrous stroma. The development of PASH is probably subject to hormonal influence so it typically affects women in the reproductive age group. Pseudoangiomatous stromal hyperplasia is frequently an incidental histologic finding in breast surgeries or biopsies performed for other injuries. In rare cases, it presents as a localized breast mass. The most important differential diagnosis is low-grade angiosarcoma. Tumorous PASH is treated by local surgical excision with clear margins. The prognosis is excellent, with minimal risk of recurrence after adequate surgery. The presented case was a 37-years-old woman who was admitted with a rapidly growing breast tumor.


Assuntos
Humanos , Adulto , Feminino , Angiomatose/cirurgia , Angiomatose/diagnóstico , Doenças Mamárias/cirurgia , Doenças Mamárias/diagnóstico , Hiperplasia/cirurgia , Hiperplasia/diagnóstico , Células Estromais/patologia
14.
J. appl. oral sci ; 21(6): 540-546, Nov-Dec/2013. tab, graf
Artigo em Inglês | LILACS | ID: lil-697813

RESUMO

Asymptomatic mouth floor enlargements may be observed in edentulous patients. These masses, which protrude from the mouth floor, may complicate the fitting of dentures and require surgery. Whether this "entity" may be considered an anatomical variation of the mouth floor or represent specific alterations in the sublingual gland is not known. Objective: The aim of this work is to investigate the morphological and morphometric aspects of the sublingual glands of edentulous patients with mouth floor enlargements and compare the glands of these patients with the sublingual glands of human cadavers. Material and Methods: Microscopic evaluation was performed on human sublingual glands from edentulous patients with mouth floor enlargements (n=20) and edentulous cadavers (n=20). The patients and cadavers were of similar ages. The data were compared using Mann-Whitney U, Fisher's exact and Student's t tests (p<0.05). Results: Acinar atrophy, duct-like structures, mononuclear infiltrates, replacement of parenchyma with fibrous/adipose tissue, mucous extravasation and oncocytosis were similar between the groups (p>0.05). Only the variables "autolysis" and "congested blood vessels" presented statistical difference between groups (p=0.014; p=0.043). The morphometric study revealed that the volume densities of acini, ducts, stroma and adipose tissue were similar between the groups (p>0.05). CONCLUSION: The microscopic characteristics of the sublingual glands in mouth floor enlargements in edentulous patients correspond to characteristics associated with the normal aging process. The glands are not pathological and represent an age-related alteration that occurs with or without the presence of the mouth floor enlargements. .


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Boca Edêntula/patologia , Glândula Sublingual/anatomia & histologia , Células Acinares/patologia , Tecido Adiposo/anatomia & histologia , Envelhecimento/fisiologia , Cadáver , Hipertrofia/patologia , Soalho Bucal , Valores de Referência , Estatísticas não Paramétricas , Células Estromais
15.
Int. braz. j. urol ; 39(3): 320-327, May/June/2013. tab, graf
Artigo em Inglês | LILACS | ID: lil-680089

RESUMO

Objective There is evidence that reactive stroma in different cancers may regulate tumor progression. The aim of this study is to establish any possible relation of reactive stroma grading on needle prostatic biopsies to biochemical recurrence. Materials and Methods The study group comprised 266 biopsies from consecutive patients submitted to radical prostatectomy. Reactive stroma was defined as stroma surrounding neoplastic tissue and graded as 0 (absent), 1 (slight), 2 (moderate), and 3 (intense) according to tumor stroma area relative to total tumor area. Results From the total of 266 needle prostatic biopsies, 143 (53.8%), 55 (20.7%), 54 (20.3%), and 14 (5.3%) showed grades 0, 1, 2, and 3, respectively. Increasing reactive stroma grade was significantly associated with clinical stage T2, higher preoperative PSA, higher biopsy and radical prostatectomy Gleason score, more extensive tumors in radical prostatectomy, and pathologic stage > T2. Only grade 3 was significantly associated with time and risk to biochemical recurrence. On multivariate analysis only preoperative PSA and 2 methods of biopsy tumor extent evaluation were independent predictors. Conclusion Increasing reactive stroma grade on biopsies is significantly associated with several clinicopathologic adverse findings, however, only grade 3 predicts time and risk to biochemical recurrence following radical prostatectomy on univariate but not on multivariate analysis. We have not been able to show that reactive stroma grade 3 on biopsies is an independent predictor of biochemical recurrence beyond that of preoperative PSA and other pathologic findings on biopsy. .


Assuntos
Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Adenocarcinoma/patologia , Recidiva Local de Neoplasia/patologia , Próstata/patologia , Prostatectomia/métodos , Neoplasias da Próstata/patologia , Células Estromais/patologia , Biomarcadores Tumorais/análise , Biópsia por Agulha Fina/métodos , Progressão da Doença , Gradação de Tumores , Valor Preditivo dos Testes , Antígeno Prostático Específico/sangue , Fatores de Risco , Análise de Sobrevida , Fatores de Tempo , Resultado do Tratamento
16.
Rev. chil. obstet. ginecol ; 78(6): 460-464, 2013. ilus
Artigo em Espanhol | LILACS | ID: lil-702354

RESUMO

La presencia de "células en anillo de sello" en el tejido ovárico es el marcador histológico clásico del tumor de Krukenberg. Un adenocarcinoma metastásico altamente agresivo y de baja sobrevida. En cambio, los fibromas ováricos son tumores del estroma generalmente benignos. Presentamos un caso muy infrecuente de fibroma celular con presencia de células en anillo de sello y revisamos los criterios para el diagnóstico diferencial con el tumor de Krukenberg.


The presence of signet-ring cells in ovarian tissue is classically described as histological marker of Krukenberg tumor. It is highly aggressive metastatic adenocarcinoma with low survival. In contrast, ovarian fibroid is a stromal tumor usually benign. We present a very rare case of cellular fibroma with presence of signet-ring cells and we review the criteria for differential diagnosis of Krukenberg tumor.


Assuntos
Humanos , Adulto , Feminino , Fibroma/diagnóstico , Neoplasias Ovarianas/diagnóstico , Tumor de Krukenberg/diagnóstico , Células Estromais/patologia , Diagnóstico Diferencial
17.
CES med ; 26(2): 175-184, jul.-dic. 2012.
Artigo em Espanhol | LILACS | ID: lil-665226

RESUMO

Objetivo: revisar la literatura disponible acerca de la fisiopatología en el desarrollo de lospólipos endometriales (EP).Metodología: se realizó una búsqueda bibliográfica en la base de datos Medline y en el buscadorPubmed, usando la palabra clave “endometrial polyps”, identificando aquella información relevanterelacionada con su fisiopatología, publicada entre los años 2000 – 2011, de artículos publicadostanto en castellano como en inglés.Resultados: se pueden resumir los fenómenos asociados con la aparición de los pólipos endometrialesen factores hormonales, inflamatorios, proliferativos y genéticos. Con respecto a la influenciahormonal se ha demostrado un aumento de los receptores de estrógenos y progestágenos, y unasobreexpresión de la aromatasa en el endometrio de los pólipos endometriales. Con respecto a losfactores inflamatorios y proliferativos, la presencia de pólipos se asocia con el aumento de la concentraciónde mastocitos activados, aumento de los niveles de cicloxigenasa 2 y el incremento de otras sustancias inhibidoras de la apoptosis, tales como, el Bcl2y el Ki67. Además, se han relacionado anormalidades genéticasen diversos cromosomas, junto con reacomodamientosproducidos por proteínas de alta movilidad, conla presencia de los EP.Conclusiones: aunque la etiología de los pólipos endometrialessigue siendo desconocida, se puede pensar quepara su aparición se requiere de un proceso proliferativoestimulado por algunos factores hormonales asociados aun fenómeno inflamatorio.


Objective: To make a review of the available literatureabout the pathophysiology of endometrialpolyps (EP) development.Methods: A search was conducted using Medlinedatabase and the browser Pubmed in orderto retrieve relevant information related to thepathophysiology of endometrial polyps, usingas search terms endometrial polyps, including publicationsfrom 2000 through 2011.Results: The factors associated with the endometrialpolyps can be summarized within hormonal,inflammatory, proliferative and geneticfactors. Related to the hormonal influence, ithas been demonstrated by an increase in estrogensand progestagen receptors, and overexpressionof aromatase in endometrial tissue ofEP. Related with inflammatory and proliferativefactors EP are associated with the increase ofmastocytes activated concentration, increase ofcycloxigenase2 levels and increase of other inhibitingapoptosis substances such as Bcl 2 andKi 67. Furthermore, EP has been associated withgenetic abnormalities in various chromosomes,together with rearrangement produced by highmobility proteins.Conclusions: In spite of the EP etiology beingunknown, it could be established that for it tooccur requires a proliferative process stimulatedby hormonal factors associated with an inflammatoryprocess.


Assuntos
Humanos , Apoptose , Hormônios , Mastócitos , Pólipos , Células Estromais
18.
Braz. oral res ; 26(4): 373-377, July-Aug. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-640715

RESUMO

Focal reactive overgrowths are among the most common oral mucosal lesions. The gingiva is a significant site affected by these lesions, when triggered by chronic inflammation in response to microorganisms in dental plaque. Myofibroblasts are differentiated fibroblasts that actively participate in diseases characterized by tissue fibrosis. The objective of this study was to evaluate the presence of stromal myofibroblasts in the main focal reactive overgrowths of the gingiva: focal fibrous hyperplasia (FFH), peripheral ossifying fibroma (POF), pyogenic granuloma (PG), and peripheral giant cell granuloma (PGCG). A total of 10 FFHs, 10 POFs, 10 PGs, and 10 PGCGs from archival specimens were evaluated. Samples of gingival mucosa were used as negative controls for stromal myofibroblasts. Oral squamous cell carcinoma samples, in which stromal myofibroblasts have been previously detected, were used as positive controls. Myofibroblasts were identified by immunohistochemical detection of alpha smooth muscle actin (α-sma). Myofibroblast immunostaining was qualitatively classified as negative, scanty, or dense. Differences in the presence of myofibroblasts among FFH, POF, PG, and PGCG were analyzed using the Kruskal-Wallis test. Stromal myofibroblasts were not detected in FFH, POF, PG, or PGCG. Consequently, no differences were observed in the presence of myofibroblasts among FFH, POF, PG, or PGCG (p > 0.05). In conclusion, stromal myofibroblasts were not detected in the focal reactive overgrowths of the gingiva that were evaluated, suggesting that these cells do not play a significant role in their pathogenesis.


Assuntos
Humanos , Crescimento Excessivo da Gengiva/patologia , Miofibroblastos/patologia , Carcinoma de Células Escamosas/patologia , Gengiva/patologia , Neoplasias Gengivais/patologia , Imuno-Histoquímica , Inclusão em Parafina , Estatísticas não Paramétricas , Células Estromais/patologia
19.
Int. braz. j. urol ; 38(3): 419-425, May-June 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-643042

RESUMO

OBJECTIVE: The purpose of this study was to compare the effects of castration on cell death rate of the adult rat prostates and to evaluate the benefic action of alpha tocopherol supplementation to avoid apoptosis post-orchiectomy. MATERIAL AND METHODS: Thirty male Wistar rats weighing 250-300g were divided into three groups: group I - they were subjected to bilateral orchiectomy and sacrificed eight weeks after the procedure; group II - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure; and group III - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the end of the experiment, the prostatectomy was performed in all rats. The presence of oxidative stress was determined by assaying the blood level of 8-isoprostane and the occurrence of apoptosis was evaluated by identification of active caspase-3 through immunohistochemical analysis. RESULTS: The statistic analysis of active caspase-3 showed that in the long-term castrated group the detection was higher than in groups were the alpha-tocopherol was supplemented (p=0.007). Analysis of 8-isoprostane levels showed higher concentrations of reactive oxygen species in group I compared to other groups (p<0.05). Groups II and III presented active caspase-3 lower than in group I (p<0.05). CONCLUSION: Our exploratory analyses demonstrate a method to study the aging process and its influence on oxidative stress of prostatic tissue and cells death rate. Based on our results we can suggest that alpha tocopherol supplementation can decrease the apoptotic process as well as the oxidative stress levels induced by androgen deprivation of the prostate gland.


Assuntos
Animais , Masculino , Ratos , Antioxidantes/farmacologia , Morte Celular/efeitos dos fármacos , Orquiectomia , Estresse Oxidativo , Próstata/citologia , alfa-Tocoferol/farmacologia , Apoptose/efeitos dos fármacos , /análise , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Ratos Wistar , Células Estromais/citologia , Fatores de Tempo , Testosterona/sangue
20.
Rev. cuba. hematol. inmunol. hemoter ; 28(1): 70-76, ene.-mar. 2012.
Artigo em Espanhol | LILACS | ID: lil-628580

RESUMO

Las células madre estromales humanas y de roedores cultivadas pueden ser inducidas a diferenciarse en neuronas, enfatizando su utilidad potencial en la terapia celular neurorrestaurativa. Los sistemas de cultivo para la expansión de estas células describen el uso de diferentes proporciones de suero fetal, lo que motivó a estudiar qué concentración de suero fetal bovino era capaz de garantizar un adecuado rendimiento celular. Las células de la médula ósea de rata se cultivaron en medio a-MEM suplementado con 10 y 20 por cientode suero fetal bovino y se subcultivaron hasta 3 veces. La viabilidad celular de los cultivos primarios y los subcultivos estuvo por encima del 98 por ciento en ambos experimentos. Los cultivos primarios demoraron 17,4 días en confluir y los subcultivos 7,7 días. La concentración de suero fetal al 20 por ciento no aumentó significativamente la velocidad de multiplicación celular; no obstante, se obtuvo un mayor número de células estromales. El sistema de expansión in vitro podría utilizarse en estudios futuros para la expansión de las células estromales humanas, lo que sienta mejores bases para su aplicación clínica


Cultured human and rodents stromal stem cells can be induced to differentiate into neurons, emphasizing its potential use in neurorestorative cell therapy. Cropping systems for the expansion of these cells describe the use of different ratios of fetal serum, which led to study what concentration of fetal calf serum was able to ensure an adequate cell yield. Cells from rat bone marrow were cultured in medium supplemented with a-MEM 10 and 20 percent fetal bovine serum and subcultured up to 3 times. Cell viability of primary cultures and subcultures was above 98 percent in both experiments. Primary cultures converge delayed in 17.4 days and 7.7 days subcultures. The concentration of 20 percent fetal calf serum did not significantly increase the speed of cell division, however, we obtained a greater number of stromal cells. The expansion in vitro system could be used in future studies for the expansion of human stromal cells, which feels better basis for clinical application


Assuntos
Células Estromais/transplante , Dispositivos para Expansão de Tecidos/normas , Ratos/genética , Soro/imunologia , Técnicas de Cultura/métodos
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