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1.
Arch. argent. pediatr ; 114(5): e319-e322, oct. 2016. ilus
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-838274

RESUMO

Las infecciones por hongos son una causa de morbilidad y mortalidad, lo que lleva a un incremento de la estancia hospitalaria y a un aumento de los costos en salud, en el período neonatal. Durante este período, los prematuros son los más afectados. Las especies Candida son la causa principal de infección fúngica. La mayoría son causadas por C. albicans, C. parapsilosis, C. glabrata y C. tropicalis, aunque otras especies han sido reportadas. Una de ellas, como un patógeno emergente, es K. ohmeri. Este organismo ha sido reportado como patógeno en el período neonatal, principalmente en prematuros. Los factores de riesgo asociados a infección fúngica son accesos venosos centrales, inmunosupresión, larga estancia hospitalaria, intubación endotraqueal y uso de antibióticos. Presentamos a un neonato con una masa mediastinal, quien requirió múltiples intervenciones, como pericardiocentesis, catéter central, ventilación mecánica y antibióticos. Durante su evolución, presentó infección por K. ohmeri. Fue tratado con anfotericina B, con evolución clínica satisfactoria.


Invasive fungal infections are a considerable cause of morbidity, mortality, increased hospital stay durations, and high health care costs, during neonatal period. In this period, the premature infants are the most affected. Candida species are the leading cause of invasive fungal infections. The majority of neonatal Candida infections are caused by C. albicans, C. parapsilosis, C. glabrata and C. tropicalis, although other fungus species are being reported. One such emerging pathogen is K. ohmeri. This organism has been reported as a pathogen in the neonatal period, principally in premature infants. The risk factors associated with fungal infection are central line, immunosuppression, prolonged hospital stay, endotracheal intubation and exposure to antibiotics. We present a term baby with a mediastinal mass, who required several procedures, as pericardiocentesis, central catheters, mechanical ventilation, antibiotics. During his evolution, he presented infection by K. ohmeri. The baby was treated with amphotericin B, with satisfactory clinical course.


Assuntos
Humanos , Masculino , Recém-Nascido , Saccharomycetales , Mediastino , Micoses/diagnóstico
3.
Braz. j. microbiol ; 45(3): 1101-1103, July-Sept. 2014. ilus
Artigo em Inglês | LILACS | ID: lil-727044

RESUMO

Ear infections in cats are uncommon, especially involving yeasts. This report describes the first isolation of the Stephanoascus ciferrii, teleomorph of the Candida genus, in a case of feline otitis in Brazil. The identification and characterization of Stephanoascus ciferrii were confirmed by the Vitek2 System (BioMerieux ®).


Assuntos
Animais , Gatos , Doenças do Gato/microbiologia , Micoses/veterinária , Otite/veterinária , Saccharomycetales/isolamento & purificação , Brasil , Microscopia , Técnicas de Tipagem Micológica , Micoses/microbiologia , Otite/microbiologia , Saccharomycetales/citologia , Saccharomycetales/crescimento & desenvolvimento , Saccharomycetales/metabolismo
4.
Braz. j. microbiol ; 44(1): 81-88, 2013. graf, tab
Artigo em Inglês | LILACS | ID: lil-676893

RESUMO

The yeast Brettanomyces/Dekkeracan cause important spoilage in wines, with the production of ethylphenols and other off-flavor compounds. This study aimed at determining the presence of this yeast and the ethylphenols produced by them in Brazilian red wines, establishing their relationship with other chemical characteristics. Isolates of Brettanomyces/Dekkerawere quantified by plating 126 samples of dry red wine in selective culture medium, while ethylphenols were analyzed by solid phase extraction and GC/FID. Free and total SO2, alcohol, total dry extract, residual sugar, total and volatile acidity, and pH were also determined. Brettanomyces/Dekkerawas present in 27% of samples. Ethylphenols were detected in most samples, with amounts higher than the threshold limit of 426 mg/L found in 46.03% of samples. The majority of wine samples showed inadequate levels of SO2and residual sugars, facts that might facilitate microbial spoilage. The passage in barrels and the grape varieties (Cabernet Sauvignon and Merlot), did not show any influence on the levels of contamination or ethylphenols contents. The prevalence of Brettanomyces/Dekkeraand the concentrations of ethylphenols were high considering the sensory impact they can cause. The growth of Brettanomyces/Dekkerawas dependent on the levels of SO2and alcohol of wines. Knowledge of the contamination, the presence of ethylphenols, and their relationship with the chemical characteristics of wines can entice effective measures to prevent Brettanomyces/Dekkeraand contribute to improve the general quality of Brazilian red wines.


Assuntos
Aromatizantes/análise , Esporos Fúngicos/crescimento & desenvolvimento , Esporos Fúngicos/isolamento & purificação , Indústria Vitivinícola/análise , Leveduras/crescimento & desenvolvimento , Leveduras/isolamento & purificação , Saccharomycetales/crescimento & desenvolvimento , Saccharomycetales/isolamento & purificação , Cromatografia Gasosa , Contaminação de Alimentos , Métodos
5.
Genet. mol. res. (Online) ; 7(1): 1-6, Jan. 2008. ilus
Artigo em Inglês | LILACS | ID: lil-553764

RESUMO

Ribonucleotide reductase (RNR) of the yeast Saccharomyces cerevisiae is a tetrameric protein complex, consisting of two large and two small subunits. The small subunits Y2 and Y4 form a heterodimer and are encoded by yeast genes RNR2 and RNR4, respectively. Loss of Y4 in yeast mutant rnr4delta can be compensated for by up-regulated expression of Y2, and the formation of a small subunit Y2Y2 homodimer that allows for a partially functional RNR. However, rnr4delta mutants exhibit slower growth than wild-type (WT) cells and are sensitive to many mutagens, amongst them UVC and photo-activated mono- and bi-functional psoralens. Cells of the haploid rnr4delta mutant also show a 3- to 4-fold higher sensitivity to the oxidative stress-inducing chemical stannous chloride than those of the isogenic WT. Both strains acquired increased resistance to SnCl2 with age of culture, i.e., 24-h cultures were more sensitive than cells grown for 2, 3, 4, and 5 days in liquid culture. However, the sensitivity factor of three to four (WT/mutant) did not change significantly. Cultures of the rnr4delta mutant in stationary phase of growth always showed higher frequency of budding cells (budding index around 0.5) than those of the corresponding WT (budding index <0.1), pointing to a delay of mitosis/cytokinesis.


Assuntos
Compostos de Estanho/toxicidade , Genes Fúngicos/genética , Mutagênicos/toxicidade , Ribonucleotídeo Redutases/genética , Saccharomyces cerevisiae/enzimologia , Sobrevivência Celular , Dimerização , Haploidia , Mutação , RNA Fúngico/biossíntese , Ribonucleotídeo Redutases/química , Saccharomycetales , Sensibilidade e Especificidade , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/genética , Fatores de Tempo
6.
Rev. cuba. aliment. nutr ; 2(2): 269-77, jul.-dic. 1988. tab
Artigo em Espanhol | LILACS | ID: lil-112234

RESUMO

Se evaluó la influencia de la ingestión de Kluyveromyces fragilis en dietas para ratas con 20 y 40 % de la levadura como fuente de proteinas sobre la actividad de las disacaridasas intestinales: lactasa, maltasa, sacarosa y trealasa en 4 niveles sucesivos de ubicación de la microvellosidad intestinal: luminal, membrana, enterocito y actividad total, para lo cual se utilizó una técnica de lavados y homogeneizados en fragmentos de intestinos tomados en la zona del ángulo de Treittz, en un total de 67 ratas agrupadas según la dieta recibida en a) grupo con pienso para roedores, b) grupo con caseina como fuente de proteinas, c)grupo con levadura al 40% durante un período experimental de 90 dias. Al comparar los resultados de las actividades enzimáticas entre los grupos estudiados, no se encontraron diferencias significativas para ninguna de las enzimas analizadas ni en ninguno de los niveles de localización en el enterocito


Assuntos
Ratos , Dissacaridases/análise , Análise de Alimentos , Proteínas na Dieta , Saccharomycetales/análise , Leveduras/análise
7.
In. Vaccari, Letizia, comp. Trabajos cientificos y discursos de incorporación a la Academia Nacional de Medicina 1915-1923; tomo II. s.l, s.n, 1984. p.233-61.
Monografia em Espanhol | LILACS | ID: lil-33503
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