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Braz. j. microbiol ; 48(2): 294-304, April.-June 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-839377


Abstract Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260 mg/L), nitrogen fixation (202.91 nmol ethylene mL-1 h-1), indole-3-acetic acid (IAA) (8.1 µg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1.34%) and oxalic acids. However, genetic evidences for nitrogen fixation and phosphate solubilization by organic acid production have been reported first time for A. aneurinilyticus strain CKMV1. A unique combination of glucose dehydrogenase (gdh) gene and pyrroloquinoline quinone synthase (pqq) gene, a cofactor of gdh involved in phosphate solubilization has been elucidated. Nitrogenase (nif H) gene for nitrogen fixation was reported from A. aneurinilyticus. It was notable that isolate CKMV1 exhibited highest antifungal against Sclerotium rolfsii (93.58%) followed by Fusarium oxysporum (64.3%), Dematophora necatrix (52.71%), Rhizoctonia solani (91.58%), Alternaria sp. (71.08%) and Phytophthora sp. (71.37%). Remarkable increase was observed in seed germination (27.07%), shoot length (42.33%), root length (52.6%), shoot dry weight (62.01%) and root dry weight (45.7%) along with NPK (0.74, 0.36, 1.82%) content of tomato under net house condition. Isolate CKMV1 possessed traits related to plant growth promotion, therefore, could be a potential candidate for the development of biofertiliser or biocontrol agent and this is the first study to include the Aneurinibacillus as PGPR.

Reguladores de Crescimento de Planta/metabolismo , Valeriana/microbiologia , Fosfatos de Cálcio/metabolismo , Lycopersicon esculentum/crescimento & desenvolvimento , Bacillales/isolamento & purificação , Fixação de Nitrogênio , Microbiologia do Solo , Cromatografia Líquida de Alta Pressão , Lycopersicon esculentum/microbiologia , Raízes de Plantas/microbiologia , Biomassa , Bacillales/metabolismo , Rizosfera , Fungos/crescimento & desenvolvimento , Antibiose
Braz. j. microbiol ; 45(4): 1271-1281, Oct.-Dec. 2014. ilus, tab
Artigo em Inglês | LILACS | ID: lil-741277


Twenty four bacterial strains from four different regions of mud volcano and lime cave were isolated to estimate their diversity, plant growth promoting and biocontrol activities to use them as inoculant strains in the fields. An excellent antagonistic effect against four plant pathogens and plant growth promoting properties such as IAA production, HCN production, phosphate solubilization, siderophore production, starch hydrolysis and hydrolytic enzymes syntheses were identified in OM5 (Pantoea agglomerans) and EM9 (Exiguobacterium sp.) of 24 studied isolates. Seeds (Chili and tomato) inoculation with plant growth promoting strains resulted in increased percentage of seedling emergence, root length and plant weight. Results indicated that co-inoculation gave a more pronounced effects on seedling emergence, secondary root numbers, primary root length and stem length, while inoculation by alone isolate showed a lower effect. Our results suggest that the mixed inocula of OM5 and EM9 strains as biofertilizers could significantly increase the production of food crops in Andaman archipelago by means of sustainable and organic agricultural system.

Bacillales/isolamento & purificação , Capsicum/microbiologia , Microbiologia Ambiental , Lycopersicon esculentum/microbiologia , Desenvolvimento Vegetal , Pantoea/isolamento & purificação , Reguladores de Crescimento de Planta/metabolismo , Biomassa , Bacillales/classificação , Bacillales/genética , Bacillales/metabolismo , Capsicum/fisiologia , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Índia , Ilhas , Lycopersicon esculentum/fisiologia , Dados de Sequência Molecular , Pantoea/classificação , Pantoea/genética , Pantoea/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , /genética , Análise de Sequência de DNA , Plântula/crescimento & desenvolvimento