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1.
Mem. Inst. Invest. Cienc. Salud (Impr.) ; 17(2): 77-85, ago. 2019. tab, ilus
Artigo em Espanhol | LILACS, BDNPAR | ID: biblio-1008512

RESUMO

La endoftalmitis infecciosa es una condición intraocular grave, aunque poco frecuente, que puede llevar a la pérdida de la visión. El diagnóstico etiológico precoz y el tratamiento adecuado llevan a una mejor evolución. Las técnicas microbiológicas convencionales tienen baja sensibilidad en el diagnóstico de la endoftalmitis infecciosa, en cambio la reacción en cadena de la polimerasa (PCR) es un método de mayor sensibilidad que está siendo utilizado ampliamente. El objetivo fue determinar por cultivo y PCR la etiología de la endoftalmitis infecciosa en pacientes que consultaron en centros oftalmológicos de Paraguay. Se estudiaron muestras de humor vítreo obtenidas por punción de 51 pacientes con endoftalmitis. La muestra se dividió en dos alícuotas; una para el estudio microbiológico convencional y la otra para la PCR anidada. Esta última utiliza un par de cebadores basado en secuencias conservadas del gen 16S ribosomal y cebadores específicos para bacterias gram positivas. Se estandarizó la PCR logrando una sensibilidad de detección de 3.10-5 ng/µl para S. aureus, 2,5.10-3 ng/µl para S. pneumoniae y 6.10-7 ng/µl para E. coli. De las 51 muestras, 12 (23,5%) fueron positivas por métodos microbiológicos convencionales y 26 (51,0%) por la PCR anidada. Por cultivo se identificaron predominantemente bacterias grampositivas 8/11 (73%) y un aislado de hongo filamentoso. La PCR anidada presentó muy buena sensibilidad y de gran utilidad en la identificación del origen bacteriano de la endoftalmitis, mejorando la sensibilidad del cultivo en 27,5%. La discriminación bacteriana en grampositivos y gramnegativos por la PCR se correlacionó con el cultivo en el 100% de los casos(AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Endoftalmite/microbiologia , Reação em Cadeia da Polimerase , Endoftalmite/diagnóstico , Estudos Transversais , Sensibilidade e Especificidade , Bactérias Gram-Positivas/genética
2.
Salud(i)ciencia (Impresa) ; 23(4): 348-350, mar. 2019.
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-1010232

RESUMO

Trueperella bernardiae es un microorganismo grampositivo facultativo anaerobio que forma parte de la microbiota normal de la piel y de la orofaringe, que recientemente ha sido reasignado l género Trueperella. Este patógeno ha sido descrito en muy pocos casos como causante de infección en los seres humanos, debido a su aspecto corineforme y su presencia en cultivos mixtos, y a las dificultades diagnósticas


Assuntos
Humanos , Actinomycetaceae , Abscesso , Infecções Bacterianas , Bactérias Gram-Positivas
3.
Braz. j. microbiol ; 49(2): 401-406, Apr.-June 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889246

RESUMO

Abstract Introduction The present study attempts to examine the microbial profile and antibiotic susceptibility of diabetic foot infections in the intensive care unit of a tertiary referral centre for diabetic foot. As part of the study, we also attempted to find the prevalence of blaNDM-like gene among carbapenem-resistant gram negative infections. Methodology A prospective study of 261 patients with diabetic foot infections was performed during the period between January 2014 and June 2014. Results A total of 289 isolates were obtained from 178 tissue samples from 261 patients, 156 (59.7%) males and 105 (40.2%) females, with a mean age of 58 years (-15 years), having diabetic foot infection. No growth was seen in thirty eight (17.6%) tissue samples. Out of the total samples, 44.3% were monomicrobial and 55.7% were polymicrobial. Gram negative pathogens were predominant (58.5%). Seven of the total isolates were fungal; 0.7% showed pure fungal growth and 1.7% were mixed, grown along with some bacteria. The most frequently isolated bacteria were Staphylococcus aureus (26.9%), followed by Pseudomonas aeruginosa (20.9%). Of the 58.5% gram negative pathogens, 16.5% were Enterobacteriaceae resistant to carbapenems. Among these isolates, 4 (25%) were positive for blaNDM-like gene. Among the rest, 18.6% were carbapenem-resistant Pseudomonas, among which 4 (36.3%) were blaNDM. Among the Staphylococci, 23.7% were methicillin-resistant Staphylococcus aureus. Conclusions Our results support the recent view that gram negative organisms, depending on the geographical location, may be predominant in DFIs. There is an increase in multidrug-resistant pathogens, especially carbapenem resistance and this is creeping rapidly. We need to be more judicious while using empiric antibiotics.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Infecções Bacterianas/epidemiologia , Pé Diabético/complicações , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Micoses/epidemiologia , Infecções Bacterianas/microbiologia , Proteínas de Bactérias/genética , beta-Lactamases/genética , Coinfecção/epidemiologia , Coinfecção/microbiologia , Bactérias Gram-Negativas/classificação , Bactérias Gram-Positivas/classificação , Índia , Resistência a Meticilina , Testes de Sensibilidade Microbiana , Micoses/microbiologia , Prevalência , Estudos Prospectivos , Centros de Atenção Terciária
4.
Braz. j. med. biol. res ; 51(6): e7118, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889111

RESUMO

In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed that the EEP of M. quadrifasciata quadrifasciata has a more complex chemical composition than the EEP of T. angustula. Moreover, UPLC-MS analyses of M. quadrifasciata quadrifascita indicated flavonoids and terpenes as major constituents. The bactericidal activity of both EEPs was higher against gram-positive bacteria than for gram-negative bacteria. The EEP from M. quadrifasciata quadrifasciata presented MIC values lower than the EEP from T. angustula for all tested bacteria. The EEP from M. quadrifasciata quadrifasciata caused lysis of the bacterial wall and release of intracellular components from both E. coli and S. aureus. Our findings indicate that the chemical composition of propolis from stingless bees is complex and depends on the species. The extract from M. quadrifasciata quadrifascita was more effective against gram-positive than gram-negative strains, especially against S. aureus and methicillin-resistant S. aureus compared to T. angustula extract, by a mechanism that involves disturbance of the bacterial cell membrane integrity.


Assuntos
Animais , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Abelhas/classificação , Bactérias Gram-Negativas/efeitos dos fármacos , Própole/química , Antibacterianos/isolamento & purificação , Antioxidantes/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Colorimetria , Bactérias Gram-Negativas/classificação , Bactérias Gram-Positivas/classificação , Testes de Sensibilidade Microbiana , Espectrometria de Massas em Tandem
5.
Braz. oral res. (Online) ; 32: e42, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889494

RESUMO

Abstract: Based on aroeira's (Myracrodruon urundeuva) antimicrobial activity and a future trend to compose intracanal medication, the aim of this study was to assess in vivo inflamatory tissue response to the extracts by edemogenic and histological analysis containing inactivated facultative and anaerobic microorganisms. For edema quantification, eighteen animals were divided into three groups (n = 3, periods: 3 and 6 hours) and 0.2 mL of 1% Evans blue per 100 g of body weight was injected into the penile vein under general anesthesia. After 30 min the animals received a subcutaneous injection in the dorsal region of aqueous or ethanolic extract of aroeira or saline (control) containing inactivated bacteria. Samples were collected, immersed in formamide for 72h, and evaluated by spectrophotometry (630 m). For histological analysis, polyethylene tubes with the extracts were implanted in the dorsal of 30 male rats. Analysis of the fibrous capsule and inflammatory infiltrate were performed after 7 and 30 days. The aqueous extract group induced less edema in both postoperative periods compared to the other groups, but the differences were not significant (p > 0.05). Tissue repair was significantly better after 30 days than after 7 days (p < 0.01). The aqueous solution showed less inflammatory response than the ethanolic solution (p < 0.05), with tendency for better results than control after 7 days. After 30 days, the response to both extracts was similar to control. The aqueous and ethanolic aroeira extracts containing inactivated microorganisms showed a trend for better results than saline, even when associated with microorganisms, and facilitated the tissue repair process.


Assuntos
Animais , Masculino , Ratos , Anacardiaceae/química , Edema/prevenção & controle , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Inflamação/prevenção & controle , Extratos Vegetais/farmacologia , Tela Subcutânea/microbiologia , Edema/patologia , Bactérias Gram-Negativas/classificação , Bactérias Gram-Positivas/classificação , Inflamação/patologia , Ratos Wistar , Tela Subcutânea/efeitos dos fármacos , Tela Subcutânea/patologia , Fatores de Tempo
6.
Braz. j. med. biol. res ; 51(3): e6872, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889038

RESUMO

The primary objective of this in vitro study was to evaluate the efficiency of removal of cariogenic bacteria and carious dentin by ablation using two lasers: fluorescence-feedback controlled (FFC) Er:YAG laser and different pulses of Er:YAG laser based on variable square pulse technology (VSPt). The secondary objective was to measure the temperature during laser ablation of carious tissue. Seventy-two extracted human molars were used in this study. Sixty teeth with carious dentin were randomly divided into four experimental groups according to the treatment for caries removal: group 1: 400 µs (FFC group); group 2: super short pulse (SSP group, 50 µs pulse); group 3: medium short pulse (MSP group, 100 µs pulse); group 4: short pulse (SP group, 300 µs pulse) and one positive control group with no treatment. Twelve teeth without carious lesion were used as a negative control group. After caries removal, swabs were taken with cotton pellets and real-time PCR analysis was performed. During caries ablation, a thermal infrared camera was used to measure the temperature changes. In all experimental groups, specimens were free of bacterial contamination after the treatment. In the SSP, MSP and SP groups, temperatures measured during caries ablation were significantly higher compared to temperatures in the FFC group (P<0.001). In this in vitro study, laser treatment for removal of carious dentin and cariogenic bacteria was an efficient treatment modality without causing excessive temperatures that might adversely affect pulp vitality.


Assuntos
Humanos , Bactérias/isolamento & purificação , Cárie Dentária/terapia , Preparo da Cavidade Dentária/métodos , Dentina/microbiologia , Lasers de Estado Sólido/uso terapêutico , Cárie Dentária/diagnóstico , Polpa Dentária/fisiologia , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Raios Infravermelhos , Reação em Cadeia da Polimerase em Tempo Real , Temperatura , Termografia
7.
Braz. dent. sci ; 21(4): 367-376, 2018.
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-965232

RESUMO

Objective: Gingivitis is caused by a mixture of Gram-positive and Gram-negative species and is characterized by inflammatory exudate in the marginal region. Probiotics have been introduced in the prevention and treatment of periodontal diseases and represent an innovative approach to maintaining oral health using beneficial bacteria when administered in adequate amounts. The mechanism of action of probiotics in the oral cavity is not fully understood but is commonly explained by a combination of local and systemic immunomodulation. The results of the studies suggest that probiotics may be useful in the prevention and treatment of gingivitis, but their effectiveness is still very questionable. Thus, more in vivo research is needed through randomized controlled studies evaluating the main periodontal clinical parameters, levels of inflammatory mediators and microbiological analyzes, especially to elucidate some still unknown mechanisms of action and to ensure the use of probiotics as an effective therapy in the prevention and treatment of gingivitis. (AU)


Objetivo: A gengivite é causada por uma mistura de espécies Gram-positivas e Gram-negativas e é caracterizada pelo exsudado inflamatório na região marginal. Os probióticos tem sido introduzido na prevenção e tratamento das doenças periodontais e representam uma abordagem inovadora para manter a saúde bucal através da utilização de bactérias benéficas quando administradas em quantidades adequadas. O mecanismo de ação dos probióticos na cavidade bucal não é totalmente compreendido, mas é comumente explicado por uma combinação de imunomodulação local e sistêmica. Os resultados dos estudos sugerem que os probióticos podem ser úteis na prevenção e tratamento da gengivite, porém sua eficácia é muito questionável ainda. Desta forma, são necessárias mais pesquisas in vivo através de estudos randomizados controlados avaliando os principais parâmetros clínicos periodontais, níveis de mediadores inflamatórios e analises microbiológicas, especialmente para elucidar alguns mecanismos de ação ainda desconhecido e para assegurar a utilização dos probióticos como terapia eficaz na prevenção e tratamento da gengivite.(AU)


Assuntos
Doenças Periodontais , Probióticos , Gengivite , Bactérias Aeróbias Gram-Negativas , Bactérias Gram-Positivas
8.
Rev. chil. cardiol ; 36(3): 221-231, dic. 2017. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-899589

RESUMO

Resumen: Hallazgos clínicos y ecocardiográficos en pacientes con endocarditis infecciosa (E.I.) asociada a catéter de hemodiálisis (CHD) fueron comparados con sujetos controles con E.I. "habitual". Pacientes y métodos: Del total de registros ecocardiográficos de una Clínica privada de Antofagasta entre 2009 y 2014, 4 pacientes presentaban E.I. asociada CHD (E.I.-CHD) y 16 otro tipo de E.I. (controles). Se compararon ambos grupos clínicamente y en las distintas modalidades de ecografía, para evaluar el aporte de cada una de ellas en el diagnóstico, manejo y comprensión del mecanismo de producción de la E.I. Resultados: El periodo del inicio de los síntomas hasta la hospitalización y el comprendido entre ésta hasta el fallecimiento fue variable, con tendencia a fallecimiento más precoz en el grupo E.I.-CHD (promedio 14,6 vs 20,6 días en el grupo control). La edad en E.I.-CHD fue mayor (65 versus 62.2 años), con mayor incidencia de las 3 comorbilidades más frecuentes: hipertensión arterial (100% versus 56,25%), diabetes mellitus (75% versus 50%) e insuficiencia cardíaca (75% versus 31,25%). La mortalidad fue 18.75% en el grupo control y 75% en la E.I.-CHD. En los pacientes con E.I.-CHD se aisló: Cándida (50%), Bacilos gram negativo (Stenotrophomonas maltophilia) (25%) y SAMR (25%) mientras que en el grupo control fueron aislados Enterococcus faecalis (25%), Streptococcus viridans (12.5%), Streptococcus spp (6.25%), Staphylococcus aureus (6.25%), Streptococcus grupo C (6.25%), Abiotrophia defectiva (6.25%) y Streptococcus pneumoniae (6.25). En 31.25% de los casos los hemocultivos fue-ron negativos. La localización más frecuente de las vegetaciones en los pacientes con E.I.-CHD correspondió a la pared de AD, sitio de impacto del jet del CHD, en un 100%, seguida por CHD 50%, tricúspide 25% y aórtica 25%. En los controles las localizaciones fueron 75% en válvula aórtica y 25% en válvula mitral. En el grupo de E.I.-CHD, la mitad presentó disfunción sistólica e insuficiencia valvular, mientras que en el grupo control fue predominante la insuficiencia valvular (62,5%) seguida por la perforación de velo (25%). Conclusión: En las E.I.-CHD se aislaron gérmenes asociados a un peor pronóstico (hongos o bacterias atípicas), con altísima mortalidad. La localización principal de las vegetaciones fue en la pared de la aurícula derecha (AD), alrededor de la desembocadura de la vena cava inferior (VCI), sitio de impacto del jet del CHD, y en el tercio distal del catéter. Clinical and echocardiographic findings in patients with infective endocarditis (I.E.) associated to the presence of hemodialysis catheters (HDC) were compared to those in subjects with other types of I.E.


Abstracts: Methods: Between 2009 and 2014 an echocardiographic diagnosis of I.E. was established in 4 subjects with a hemodialysis catheter in place and 16 patients had a common variety of I.E. (controls). Clinical and echocardiographic findings, including echocardiographic modalities were compared between groups. Results: Time from onset of symptoms and time to patient death were shorter in I.E.-HDC patients compared to controls (mean 14.6 vs 20.6 days, respectively. I.E. -HDC patients tended to be older (65.0 vs 62.2 years old). The incidence of main comorbidities was higher in I.E.-HDC: hypertension (100% vs 56.2%), diabetes (75 vs 59%) and heart failure (75% vs 31.2%), respectively. Mortality was much higher in I.E-HDC (74% vs 18%). Infective agents also differed between groups: I.E.-HDC was associate to C albicans (50%), Gram negative rods (Stenotrophomonas maltophilia) (25%) y SAMR (25%). In contrast, the agents involved in controls were E faecalis (25%), S viridans (12.5%), S. group C (6.25%), S aureus (6,25%), group C Streptococcus (6.25%), Abiotrophic defectiva (6.25%) and S pneumoniae (6.25%). Overall, blood cultures were negative in 31.2% patients. Among patients with I.E.-HDC vegetations were most commonly found at the right atrial wall (100%), on the catheter (50%), at the tricuspid valve (25%) and at the aortic valve (25%). In non I.E.-HDC, vegetations were located at the aortic valve (75%) and the mitral valve (25%). Half the patients with I.E.- HDC patients presented ventricular dysfunction and valvular regurgitation while control patients had predominantly valvular insufficiency (62.5%) or leaflet perforation (25%). Conclusion: Mortality in I.E.-HDC patients was very high, associated to the presence of aggressive microbial or fungal agents. Vegetations were most commonly located at the right atrial wall, around de junction with the inferior vena cava and on the catheter itself.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Diálise Renal/efeitos adversos , Endocardite/microbiologia , Endocardite/diagnóstico por imagem , Infecções Relacionadas a Cateter/complicações , Candida/isolamento & purificação , Ecocardiografia , Comorbidade , Estudos Retrospectivos , Infecções Relacionadas a Cateter/microbiologia , Infecções Relacionadas a Cateter/diagnóstico por imagem , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação
9.
Mem. Inst. Oswaldo Cruz ; 112(10): 692-697, Oct. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-894841

RESUMO

BACKGROUND Endophytic fungi, present mainly in the Ascomycota and Basidiomycota phyla, are associated with different plants and represent important producers of bioactive natural products. Brazil has a rich biodiversity of plant species, including those reported as being endemic. Among the endemic Brazilian plant species, Vellozia gigantea (Velloziaceae) is threatened by extinction and is a promising target to recover endophytic fungi. OBJECTIVE The present study focused on bioprospecting of bioactive compounds of the endophytic fungi associated with V. gigantea, an endemic, ancient, and endangered plant species that occurs only in the rupestrian grasslands of Brazil. METHODS The capability of 285 fungal isolates to produce antimicrobial and antimalarial activities was examined. Fungi were grown at solid-state fermentation to recover their crude extracts in dichloromethane. Bioactive extracts were analysed by chromatographic fractionation and NMR and displayed compounds with antimicrobial, antimycobacterial, and antimalarial activities. FINDINGS Five fungi produced antimicrobial and antimalarial compounds. Extracts of Diaporthe miriciae showed antifungal, antibacterial, and antimalarial activities; Trichoderma effusum displayed selective antibacterial activity against methicillin-resistant Staphylococcus aureus and Mycobacterium intracellulare; and three Penicillium species showed antibacterial activity. D. miriciae extract contained highly functionalised secondary metabolites, yielding the compound epoxycytochalasin H with high antimalarial activity against the chloroquine-resistant strain of Plasmodium falciparum, with an IC50 approximately 3.5-fold lower than that with chloroquine. MAIN CONCLUSION Our results indicate that V. gigantea may represent a microhabitat repository hotspot of potential fungi producers of bioactive compounds and suggest that endophytic fungal communities might be an important biological component contributing to the fitness of the plants living in the rupestrian grassland.


Assuntos
Plasmodium/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Magnoliopsida/classificação , Magnoliopsida/microbiologia , Fungos Mitospóricos/efeitos dos fármacos , Bactérias Aeróbias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antifúngicos/isolamento & purificação , Antifúngicos/farmacologia , Antimaláricos/isolamento & purificação , Antimaláricos/farmacologia , Clima Tropical , Bioensaio , Candida/efeitos dos fármacos , Endófitos/química
10.
Mem. Inst. Oswaldo Cruz ; 112(8): 537-543, Aug. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-894863

RESUMO

BACKGROUND Silver nanoparticles (AgNPs) are increasingly being used in medical applications. Therefore, cost effective and green methods for generating AgNPs are required. OBJECTIVES This study aimed towards the biosynthesis, characterisation, and determination of antimicrobial activity of AgNPs produced using Pseudomonas aeruginosa ATCC 27853. METHODS Culture conditions (AgNO3 concentration, pH, and incubation temperature and time) were optimized to achieve maximum AgNP production. The characterisation of AgNPs and their stability were evaluated by UV-visible spectrophotometry and scanning electron microscopy. FINDINGS The characteristic UV-visible absorbance peak was observed in the 420-430 nm range. Most of the particles were spherical in shape within a size range of 33-300 nm. The biosynthesized AgNPs exhibited higher stability than that exhibited by chemically synthesized AgNPs in the presence of electrolytes. The biosynthesized AgNPs exhibited antimicrobial activity against Escherichia coli, P. aeruginosa, Salmonella typhimurium, Staphylococcus aureus, methicillin-resistant S. aureus, Acinetobacter baumannii, and Candida albicans. MAIN CONCLUSION As compared to the tested Gram-negative bacteria, Gram-positive bacteria required higher contact time to achieve 100% reduction of colony forming units when treated with biosynthesized AgNPs produced using P. aeruginosa.


Assuntos
Humanos , Prata/farmacologia , Contagem de Colônia Microbiana/métodos , Nanopartículas Metálicas/química , Bactérias Gram-Negativas/metabolismo , Bactérias Gram-Negativas/ultraestrutura , Bactérias Gram-Positivas/efeitos dos fármacos , Antibacterianos/biossíntese , Antibacterianos/farmacologia , Antibacterianos/química , Pseudomonas aeruginosa , Espectrofotometria , Microscopia Eletrônica/métodos
11.
Autops. Case Rep ; 7(2): 27-34, Apr.-June 2017. ilus
Artigo em Inglês | LILACS | ID: biblio-905205

RESUMO

Firstly described in the 19th century by Sir William Osler, the mycotic aneurysm (MA) is a rare entity characterized by an abnormal arterial dilation, which is potentially fatal, and is associated with the infection of the vascular wall. Elderly patients are mostly involved, especially when risk factors like chronic diseases, immunosuppression, neoplasia, and arterial manipulation are associated. The authors report the case of a young male patient diagnosed with an aortic aneurysm of infectious origin in the presence of repeated negative blood cultures. The diagnostic hypothesis was raised when the patient was hospitalized for an inguinal hernia surgery. The diagnosis was confirmed based on imaging findings consistent with mycotic aneurism. The patient was treated with an endovascular prosthesis associated with a long-lasting antibiotic therapy. Five months later, the patient attended the emergency unit presenting an upper digestive hemorrhage and shock, from which he died. The autopsy revealed a huge aneurysm of the abdominal aorta with an aortoduodenal fistula. The histological examination of the arterial wall revealed a marked inflammatory process, extensive destruction of the arterial wall, and the presence of Gram-positive bacteria. This case highlights the atypical presentation of a MA associated with an aortoduodenal fistula. Besides the early age of the patient, no primary arterial disease could be found,and no source of infection was detected.


Assuntos
Humanos , Masculino , Adulto , Aneurisma Infectado/diagnóstico , Aneurisma Aórtico/diagnóstico , Fístula/patologia , Aneurisma Infectado/tratamento farmacológico , Aorta Abdominal/patologia , Autopsia , Evolução Fatal , Hemorragia Gastrointestinal/diagnóstico , Bactérias Gram-Positivas , Hérnia Inguinal/diagnóstico , Choque/diagnóstico
12.
Iatreia ; 30(1): 5-20, ene.-mar. 2017. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-892638

RESUMO

RESUMEN Introducción: para el tratamiento de las infecciones por Enterococcus resistente a vancomicina (ERV) se emplean fármacos de segunda línea como daptomicina y linezolid. Objetivo: hacer una revisión sistemática para evaluar el tratamiento de la bacteriemia por ERV, con daptomicina o linezolid. Metodología: búsqueda electrónica en las bases de datos de Pubmed, Embase, Scopus, ScienceDirect, CENTRAL, Lilacs y Google Académico, para identificar estudios anteriores a julio de 2015 que hayan comparado los tratamientos con daptomicina o linezolid de pacientes infectados por ERV. Resultados: se incluyeron 15 estudios de 1307 registros. No hubo diferencias entre daptomicina y linezolid con respecto a la mortalidad a 30 días. Con la daptomicina se logró más tempranamente el control microbiológico (OR: 0,64; IC95 %: 0,45-0,92). No hubo diferencias entre los dos antibióticos en cuanto a la mejoría clínica, la necesidad de admisión a la UCI o la aparición de efectos adversos como trombocitopenia, neutropenia e insuficiencia renal. Conclusiones: no encontramos diferencias entre daptomicina y linezolid en cuanto a la mortalidad de pacientes infectados por ERV, aunque con la daptomicina se logró una cura microbiológica más rápida.


SUMMARY Introduction: Second-line drugs such as linezolid and daptomycin are used for treatment of vancomycin-resistant Enterococcus (VRE) infections. Objective: A systematic review to evaluate treatment of VRE bacteremia with linezolid versus daptomycin. Methods : A search was done in the databases of Pubmed, Embase, Scopus, ScienceDirect, CENTRAL, Lilacs and Google Scholar to identify studies comparing treatment with daptomycin or linezolid of patients infected by VRE up to July 2015. Result: Only 15 studies were included of a total of 1.307 records. There were no differences between daptomycin and linezolid with respect to mortality at 30 days. Microbiological cure was better with daptomycin (OR: 0.64; 95 % CI: 0.45-0.92), whereas there was no difference between the two antibiotics with respect to clinical cure, need to ICU admission, and the occurrence of adverse effects such as thrombocytopenia, neutropenia and renal failure. Conclusions: No significant differences were observed between daptomycin and linezolid in reference to mortality of patients infected with VRE, although daptomycin treatment produced a faster microbiological cure.


RESUMO Introdução: para o tratamento das infecções por Enterococcus resistente a vancomicina (ERV) se empregam fármacos de segunda linha como daptomicina e linezolida. Objetivo: fazer uma revisão sistemática para avaliar o tratamento da bacteriemia por ERV, com daptomicina o linezolida. Metodologia: busca eletrônica nas bases de dados de Pubmed, Embase, Scopus, ScienceDirect, CENTRAL, Lilacs e Google Acadêmico, para identificar estudos anteriores a julho de 2015 que foram comparados os tratamentos com daptomicina ou linezolida de pacientes infectados por ERV. Resultados: se incluíram 15 estudos de 1.307 registros. Não houve diferenças entre daptomicina e linezolida com respeito à mortalidade a 30 dias. Com a daptomicina se conseguiu mais precoce o controle microbiológico (OR: 0,64; IC95 %: 0,45-0,92). Não houve diferenças entre os dois antibióticos em quanto à melhoria clínica, a necessidade de admissão à UTI ou a aparição de efeitos adversos como trombocitopenia, neutropenia e insuficiência renal. Conclusões: não encontramos diferenças entre daptomicina e linezolida em quanto à mortalidade de pacientes infectados por ERV, embora com a daptomicina se conseguiu uma cura microbiológica mais rápida.


Assuntos
Humanos , Anti-Infecciosos , Enterococcus , Bactérias Gram-Positivas
13.
Rev. Salusvita (Online) ; 36(3): 695-707, 2017.
Artigo em Português | LILACS | ID: biblio-1021583

RESUMO

Introdução: os agentes responsáveis por infecções graves em neonatais são geralmente oriundos da mãe, tendo como o grupo mais grave e predominante os Streptococcus do grupo B (EGB). Estas bactérias Gram-positivas, normalmente presentes no trato gastrointestinal, podem ser transmitidas da mãe para o feto através de transmissão vertical, gerando graves doenças em neonatos, com taxas de mor talidade de 50% e de colonização de aproximadamente 6 a 8% das gestantes. Objetivos: este estudo avaliou a prevalência de gestantes portadoras de Streptococcus agalactiae, atendidas no Laboratório de Análises Clínicas da Fundação Véritas da Universidade do Sagrado Coração no município de Bauru no período de 2013 a 2015. Material e Métodos: foram analisados laudos de exames de cultura de secreção vaginal de pacientes gestantes disponíveis no sistema Pleres, utilizando um formulário para coleta das informações. Resultados: os resultados demonstraram que no período de 2013 a 2015, das 560 culturas de secreção vaginal realizadas para pacientes gestantes cerca de 4,3% apresentaram-se positivas para S. agalactiae, sendo este percentual composto em sua maioria por atendimentos originários do Sistema Único de Saúde (SUS), além da prevalência de gestantes entre 20 e 29 anos. Dentre os antimicrobianos avaliados nos testes de suscetibilidade observou-se que S. agalactiae foi resistente, especialmente, à clindamicina. Conclusão: foram encontradas taxas de prevalência de S. agalactiae em gestantes menores que as médias observadas em outros estudos nacionais, com perfil de sensibilidade reduzido frente à clindamicina.


Introduction: the agents responsible for the serious infections in newborns are generally coming in the mother, having the Group B Streptococcus (GBS) as the most severe and prevalent group. These agents are Gram-positive bacteria that are normally found in the gastrointestinal tract and can be transmitted from the mother to the unborn baby by vertical transmission, which generates many severe diseases in mothers who have just given birth and newborn babies, with mortality rates at 50%, these bacteria colonize approximately 6 to 8% of pregnant women. Objectives: this epidemiological study aimed to evaluate the prevalence of pregnant women bearing Streptococcus agalactiae (EGB) in their birth canal and who were taken care of at the Fundação Véritas Clinical Analysis Laboratory of Universidade do Sagrado Coração in the city of Bauru, during the period 2013/2015. Material and methods: we analyzed exam reports of vaginal secretion culture tests for GBS of pregnant patients available in the Pleres system, using a form for collecting the information. Results and Discussion: the results showed that of 560 cultures performed during this period, 4.3% had S. agalactiae isolated, being that most of the patients were admitted by the Brazilian Sistema Único de Saúde (SUS) and had ages ranging from 20 to 29 years old. Among the antibiotics evaluated in the sensitivity tests, S. agalactiae proved to be resistant, especially, to clindamycin. Conclusion: it is believed that the decrease in the percentage of prevalence of S. agalactiae in this study in comparison to other researches was due to the changes in public policies and the implementation of screening protocols from 2013 for ANVISA (Agência Nacional de Vigilância Sanitária).


Assuntos
Gravidez , Streptococcus agalactiae , Infecções por Bactérias Gram-Positivas , Gestantes , Bactérias Gram-Positivas
14.
Rev. Investig. Salud. Univ. Boyacá ; 4(1): 38-52, 2017. tab
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-910752

RESUMO

Introducción. La presencia de especies de Listeria spp. en alimentos, se ha convertido en un problema de salud pública, considerando su capacidad patógena, especialmente la de L. monocytogenes. Objetivo. Determinar el perfil de sensibilidad de cepas de Listeria spp. aisladas de muestras de leche cruda de vaca en fincas del municipio de Tunja, Boyacá. Metodología. Se analizaron 293 muestras de leche cruda de vaca obtenidas de las cantinas de almacena-miento; para el aislamiento y la identificación bioquímica de Listeria spp., se hizo preenriquecimiento en caldo Fraser (Oxoid), incubación a 4 °C, aislamiento en medio Palcam (Oxoid) e identificación utilizando Microbact Listeria L-12 (Oxoid). El perfil de sensibilidad a los antibióticos se determinó por el método de difusión de disco. Resultados. La prevalencia total de Listeria spp. fue de 9,89 % (n=29). La especie más prevalente fue L. seeligeri (7,17 %) y la menos prevalente, L. grayi (0,34 %). Listeria monocytogenes presentó una prevalencia de 2,7 %. Se determinó que todos los aislamientos de Listeria spp. eran sensibles a la penicilina y a la ampicilina. Un aislamiento de L. monocytogenes fue resistente a ciprofloxacina, gentamicina, trimetoprim-sulfametoxazol y tetraciclina, mientras que las demás fueron sensibles a los antibióticos evaluados. Conclusiones. Se determinó la presencia de varias especies de Listeria spp., incluyendo L. monocytogenes, la cual se considera de importancia en salud pública. Presentaron sensibilidad a la mayoría de los antibióticos analizados, excepto a clindamicina; asimismo, se reporta un caso aislado de resistencia a tetraciclina en una cepa de L. monocytogenes y se determinó la resistencia a trimetoprim-sulfametoxazol.


Introduction: The presence of the Listeria species in food has become a public health concern, consi-dering its pathogenicity, especially due L. monocytogenes. Objective: This study aimed to determine the susceptibility of Listeria spp. strains isolated from raw cow's milk samples on farms of the municipality of Tunja, Boyacá. Methodology: Two hundred and ninety three raw cow's milk samples were analyzed, obtained from bulk milk cooling tanks; isolation and identification of Listeria spp. using pre-enrichment in Fraser supplement (Oxoid), incubation at 4° C, isolation in the Palcam medium (Oxoid) and distinguishing by means of Microbact Listeria L-12 (Oxoid) was carried out. The sensitivity profile was determined by the disk diffusion method. Results: The total prevalence of Listeria spp. was 9.89% (n=29). Listeria seeligeri was the most prevalent species (7.17%) and L. gravi was the less prevalent (0.34%). Prevalence of L. monocytogeneswas 2.7%. It was determined that all Listeria spp. isolates were susceptible to penicillin and ampicillin. An isolation of L. monocytogenes was resistant to ciprofloxacin, gentamicin, trimethoprim-sulfame-thoxazole and tetracycline, while the others were sensitive to the antibiotics evaluated. Conclusions: The presence of several species of Listeria was determined, including L. monocyto-genes, which is considered of importance in public health. Susceptibility to most of the antibiotics analyzed except for clindamycin was reported. In addition, an isolated case of tetracycline resistance was reported in a strain of L. monocytogenes and resistance to trimethoprim-sulfamethoxazole was determined.


Introdução. A presença de espécies de Listeria spp. em alimentos, tornou-se um problema de saúde pública, considerando sua capacidade patogênica, especialmente na L. monocytogenes. Objetivo. Determinar o perfil de sensibilidade da linhagem Listeria spp. isolada a partir de amostras de leite de vaca cru em fazendas do município de Tunja, Boyaca. Metodologia. Foram analisadas 293 amostras de leite de vaca cru obtidas a partir dos barris de arma-zenamento de leite; para o isolamento e identificação bioquímica da Listeria spp., foi realizado um pré-enriquecimento em Fraser Broth (Oxoid), incubação a 4 °C, isolamento em meio Palcam (Oxoid) e identificação usando Microbact Listeria G-12 (Oxoid). O perfil de sensibilidade aos antibióticos foi determinada pelo método de difusão em disco. Resultados. A prevalência total da Listeria spp. foi de 9,89% (n = 29). A linhagem com maior preva-lencia foi da L. seeligeri (7,17%) e com menor prevalencia L. grayi (0,34%). Listeria monocytogenes mostrou uma prevalência de 2,7%. Todos os isolados de Listeria spp. foram sensíveis à penicilina e ampicilina. Um isolamento de L. monocytogenes foi resistente ao antibiótico ciprofloxacina, gentami-cina, trimetoprim-sulfametoxazol e tetraciclina, enquanto outros forma sensíveis a estes antibióticos. Conclusões. Determinou-se a presença de várias espécies de Listeria spp., incluindo L. monocytogenes, considerada de importância na saúde pública. As linhagens foram sensíveis à maioria dos antibióticos testados excepto clindamicina; Além disso, um isolado foi resistente a tetraciclina na linhagem L. monocytogenes e foi determinada a resistência a trimetoprim-sulfametoxazol.


Assuntos
Animais , Laticínios , Inspeção de Alimentos , Inocuidade dos Alimentos , Bactérias Gram-Positivas
15.
Rev. Salusvita (Online) ; 36(2): 365-379, 2017. tab
Artigo em Português | LILACS | ID: biblio-876496

RESUMO

Introdução: há uma busca incessante por agentes fitoterápicos na medicina e odontologia. Dentro da endodontia, busca-se uma substância que potencialize os efeitos biológicos e antimicrobianos do hidróxido de cálcio como curativo de demora. Não há na literatura relatos sobre a associação do cimento Portland com a Bambusa textilis, uma planta nativa da China, onde é usada na elaboração de medicamentos, pois se trata de uma fonte importante de resinas, sendo, portanto utilizadas por seu poder anti-inflamatório, anticatarral, anticonvulsivante e antitérmico. Sabendo da semelhança do cimento Portland com o MTA, e que esse ultimo apresenta bons resultados físico-químicos, surge a dúvida da possibilidade da associação, com a finalidade de melhorar as atividades antimicrobianas, e a interferência na liberação de íons cálcio e hidroxila. Objetivo: avaliar o pH, liberação de íons cálcio e atividade antimicrobiana do cimento Portland associado a Bambusa textilis em diferentes proporções. Metodologia: os cimentos experimentais foram 3 g Cimento Portland + 1,2 ml de Bambusa textilis (G1); 3 g Cimento Portland + 0,6 ml de água + 0,6 de Bambusa textilis (G2); 3 g Cimento Portland + 0,9 ml água + 0,3 ml de Bambusa textilis (G3); 3 g Cimento Portland puro (controle) + 1,2 ml água (G4); Bambusa textilis (controle): 20 microlitros por poço. Para os testes de pH e liberação de íons cálcio foram utilizados tubos de polietileno, os quais foram preenchidos com os cimentos e imersos em frascos contendo água deionizada. As mensurações foram por meio de um peagâmetro e espectrofotômetro de absorção atômica. Para a análise da atividade antimicrobiana, foi utilizada a técnica de difusão radial com cepa de Enterococcus faecalis. Os halos de inibição foram mensurados com auxílio de um paquímetro digital. A análise estatística foi realizada pelo teste ANOVA para comparação global, e teste de Tukey para comparações individuais, com significância de 5%. Resultados: os valores de pH se mostraram próximos ao neutro para todos os grupos em todos os períodos experimentais. Houve liberação de íons cálcio e ocorreu em todos os grupos e em todos os períodos experimentais. Em relação à atividade antimicrobiana, nenhuma das proporções apresentou atividade. Conclusão: a associação da Bambusa textilis ao cimento Portland manteve seu pH e liberação de íons cálcio, mas não potencializou sua atividade antimicrobiana frente ao E. faecalis. (AU)


Introduction: there is a search for phytotherapeutic agents in medicine and dentistry. Within endodontics, a substance that potentiates the biological and antimicrobial effects of calcium hydroxide as a delay dressing is sought. There are no reports in the literature about the association of Portland cement with Bambusa textilis, a plant native to China, where it is used in the manufacture of medicines, since it is an important source of resins and is therefore used for its anti-inflammatory power, anticonvulsive and antipyretic. Knowing the similarity of the Portland cement with the MTA, and that the latter presents good physicochemical results, the possibility of the association arises, with the purpose of improving the antimicrobial activities, and the interference in the release of calcium and hydroxyl ions. Aim: to analyze the pH, calcium ion release and antibacterial activity of Portland cement associated to Bambusa textilis in different proportions. Methods: the experimental cements ́ proportions were: 3 g of Portland cement + 1.2 ml of Bambusa textilis (G1); 3 g of Portland cement + 0.6 ml of water + 0.6 of Bambusa textilis (G2); 3 g of Portland cement + 0.9 ml of water + 0.3 ml of Bambusa textilis (G3); 3 g of pure Portland cement (control group) + 1.2 ml of water (G4); Bambusa textilis (control group): 20 µLper well. Polyethylene tubes filled with the cements and immersed in deionized water were employed. Calcium ion release was analyzed using an atomic absorption spectrophotometer and the change in pH was determined through a pH meter. For the analysis of antibacterial activity, a digital radial diffusion assay with a Enterococcus faecalis strain was utilized. Statistical analysis was performed using ANOVA and Tukey's tests. The level of significance was set at P = 0.05. Results: pH values were close to neutral for all of the experimental groups in all periods evaluated. Calcium ion release was observed in all experimental groups and evaluation periods. Regarding the antibacterial activity, this property could not be detected in any of the proportions used in this study. Conclusions: the association of Bambusa textilis to Portland cement, preserved its pH values and its calcium ion release property. Nevertheless, it did not improve its antibacterial activity against E. faecalis strains. (AU)


Assuntos
Animais , Bactérias Gram-Positivas
16.
Clinics ; 71(12): 715-719, Dec. 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-840025

RESUMO

OBJECTIVES: To clinically and epidemiologically characterize a population diagnosed with and treated for septic arthritis of the knee, to evaluate the treatment results and to analyze the differences between patients with positive and negative culture results, patients with Gram-positive and Gram-negative bacterial isolates and patients with S. aureus- and non-S. aureus-related infections. METHODS: One hundred and five patients with septic knee arthritis were included in this study. The clinical and epidemiological data were evaluated. Statistical analysis was performed to compare patients with and without an isolated causative agent, patients with Gram-positive and Gram-negative pathogens and patients with S. aureus-related and non S. aureus-related infections. RESULTS: Causative agents were isolated in 81 patients. Gram-positive bacteria were isolated in 65 patients and Gram-negative bacteria were isolated in 16 patients. The most commonly isolated bacterium was S. aureus. Comparing cases with an isolated pathogen to cases without an isolated pathogen, no differences between the studied variables were found except for the longer hospital stays of patients in whom an etiological agent was identified. When comparing Gram-positive bacteria with Gram-negative bacteria, patients with Gram-positive-related infections exhibited higher leukocyte counts. Patients with S. aureus-related infections were more frequently associated with healthcare-related environmental encounters. CONCLUSION: S. aureus is the most common pathogen of septic knee arthritis. Major differences were not observed between infections with isolated and non-isolated pathogens and between infections with Gram-positive and Gram-negative bacteria. S. aureus infections were more likely to be associated with a prior healthcare environment exposure.


Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Adulto Jovem , Artrite Infecciosa/microbiologia , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Articulação do Joelho/microbiologia , Artrite Infecciosa/epidemiologia , Brasil , Proteína C-Reativa/análise , Joelho/microbiologia , Estudos Retrospectivos , Distribuição por Sexo , Infecções Estafilocócicas/epidemiologia , Staphylococcus aureus/isolamento & purificação
17.
Braz. j. pharm. sci ; 52(3): 471-482, July-Sept. 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-828278

RESUMO

ABSTRACT A series of molecules bearing multiple functional groups were synthesized to study their antibiotic effect against Gram-positive and Gram-negative bacteria and lipoxygenase activity as well. 2,4-Dimethylcarbolic acid (1) was refluxed with ethyl 2-bromoacetate to synthesize ethyl 2-(2,4-dimethylphenoxy)acetate (2). Compound 2 was converted to the corresponding hydrazide 3, again on refluxing with hydrazine. The compound 5-((2,4-dimethylphenoxy)methyl)-1,3,4-oxadiazol-2-thiol (4) was synthesized by the reaction of 3 and CS2 in the presence of KOH. Compound 4 was further converted to the corresponding ester 5 and then 2-(5-((2,4-dimethylphenoxy)methyl)-1,3,4-oxadiazol-2-ylthio)acetohydrazide (6). The final molecules N'-substituted-2-(5-((2,4-dimethylphenoxy)methyl)-1,3,4-oxadiazol-2-ylthio)acetohydrazide, 8a-m, bearing ether, 1,3,4-oxadiazole, thioether, hydrazone and azomethine functional groups were synthesized by stirring the aryl carboxaldehydes 7a-m with 6 in methanol at room temperature. The depicted structures of all synthesized molecules were corroborated by IR, 1H-NMR and EIMS spectral data analysis. 8m and 8i showed substantial antibacterial activity and lipoxygenase inhibitory activity, respectively.


Assuntos
Oxidiazóis/análise , Análise Espectral/estatística & dados numéricos , Bactérias Gram-Negativas , Bactérias Gram-Positivas , Lipoxigenases/análise
18.
Mem. Inst. Oswaldo Cruz ; 111(4): 241-251, Apr. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-779002

RESUMO

Localised cutaneous leishmaniasis (LCL) is the most common form of cutaneous leishmaniasis characterised by single or multiple painless chronic ulcers, which commonly presents with secondary bacterial infection. Previous culture-based studies have found staphylococci, streptococci, and opportunistic pathogenic bacteria in LCL lesions, but there have been no comparisons to normal skin. In addition, this approach has strong bias for determining bacterial composition. The present study tested the hypothesis that bacterial communities in LCL lesions differ from those found on healthy skin (HS). Using a high throughput amplicon sequencing approach, which allows for better populational evaluation due to greater depth coverage and the Quantitative Insights Into Microbial Ecology pipeline, we compared the microbiological signature of LCL lesions with that of contralateral HS from the same individuals.Streptococcus, Staphylococcus,Fusobacterium and other strict or facultative anaerobic bacteria composed the LCL microbiome. Aerobic and facultative anaerobic bacteria found in HS, including environmental bacteria, were significantly decreased in LCL lesions (p < 0.01). This paper presents the first comprehensive microbiome identification from LCL lesions with next generation sequence methodology and shows a marked reduction of bacterial diversity in the lesions.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Leishmaniose Cutânea/microbiologia , Pele/microbiologia , Bactérias Gram-Negativas/classificação , Bactérias Gram-Positivas/classificação , Pele/parasitologia
19.
Pesqui. vet. bras ; 36(4): 279-282, tab
Artigo em Português | LILACS | ID: lil-787576

RESUMO

Objetivou-se avaliar a atividade antibacteriana in vitro da própolis marrom, por meio da determinação da Concentração Inibitória Mínima (CIM). O extrato alcoólico de própolis foi obtido de 35 g de própolis bruta macerada em 65 mL de álcool de cereais. As concentrações do extrato de própolis usadas foram: 75mg/mL; 56,4mg/mL; 37,5mg/mL; 18,9mg/mL; 9,3mg/mL; 4,5mg/mL e 2,25mg/mL. Foram utilizados 32 isolados de bactérias Gram-positivas: Rhodococcus equi, Staphylococcus aureus, Staphylococcus hyicus, Staphylococcus spp. e Streptococcus spp., e 32 isolados de bactérias Gram-negativas: Enterobacter agglomerans, Escherichia coli, Klebsiella pneumoniae, Klebsiella sp., Pseudomonas aeruginosa, Pseudomonas spp., Salmonella spp. e Serratia rubidaea, provenientes de processos clínicos infecciosos de animais domésticos, obtidas e armazenadas no Laboratório de Bacteriologia da FAMEZ/UFMS. O extrato de própolis marrom apresentou atividade antimicrobiana com CIM variando de 2,25 a 18,9mg/mL para as bactérias Gram-positivas e 4,5 a 18,9mg/mL para as bactérias Gram-negativas, sendo as bactérias provenientes de bovinos e caninos as mais resistentes. Conclui-se que a própolis marrom tem ação bactericida, em função da espécie da bactéria e da procedência animal.


The aim of this study was to evaluate the in vitro antibacterial activity of brown propolis, by determining the minimum inhibitory concentration (MIC). The alcoholic extract of propolis was obtained from 35g of crude propolis macerated in 65mL of cereals alcohol. Dilutions of propolis extracts used were: 75mg/mL; 56.4mg/mL; 37.5mg/mL; 18.9mg/mL; 9.3mg/mL; 4.5mg/mL, and 2.25mg/mL. Thirty-two Gram-positive bacteria isolates were used: Rhodococcus equi, Staphylococcus aureus, Staphylococcus hyicus, Staphylococcus spp. and Streptococcus spp.; and the thirty-two Gram-negative bacteria isolated were: Enterobacter agglomerans, Escherichia coli, Klebsiella pneumoniae, Klebsiella sp., Pseudomonas aeruginosa, Pseudomonas sp., Salmonella sp., and Serratia rubidaea, obtained from infectious clinical processes of domestic animals, and stored in the Laboratory of Bacteriology of FAMEZ/UFMS. The brown propolis extract showed antibacterial activity with MIC ranging from 2.25 to 18.9mg/ml for Gram-positive bacteria and 4.5 to 18.9mg/ml for Gram-negative bacteria, and bacteria from cattle and dogs were more resistant. It was concluded that brown propolis has antibacterial action, but the effect depends on the species of the bacterium and its origin.


Assuntos
Antibacterianos/análise , Própole/análise , Própole/uso terapêutico , Bactérias Gram-Negativas , Bactérias Gram-Positivas , Técnicas In Vitro , Testes de Sensibilidade Microbiana
20.
Braz. j. pharm. sci ; 52(1): 77-85, Jan.-Mar. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-789071

RESUMO

ABSTRACT Keeping in mind the pharmacological importance of the 1,3,4-oxadiazole moiety, a series of new S-substituted derivatives, 5a-h, of 5-(1-(4-tosyl)piperidin-4-yl)-1,3,4-oxadiazol-2-thiol (3) were synthesized. The reaction of p-toluenesulfonyl chloride (a) and ethyl isonipecotate (b) produced ethyl 1-(4-tosyl)piperidin-4-carboxylate (1) which was further transformed into 1-(4-tosyl)piperidin-4-carbohydrazide (2) by hydrazine hydrate in methanol. Compound 2 was refluxed with CS2 in the presence of KOH to synthesize 5-(1-(4-tosyl)piperidin-4-yl)-1,3,4-oxadiazol-2-thiol (3). The desired compounds, 5a-h, were synthesized by stirring 3 with aralkyl halides, 4a-h, in DMF using NaH as an activator. The structures of synthesized compounds were elucidated by 1H-NMR, IR and EI-MS spectral studies. These compounds were further evaluated for enzyme inhibitory activity against lipoxygenase and alpha-glucosidase, along with antibacterial activity against Gram-negative and Gram-positive bacteria.


RESUMO Tendo em vista a importância farmacológica da porção 1,3,4-oxadiazol, sintetizou-se uma série de novos derivados S-substituídos, 5a-h, de 5-(1-(4-tosi)piperidin-4-il)-1,3,4-oxadiazol-2-tiol (3). A reação do cloreto de p-toluenossulfonila (a), com isonipecotato de (b) etila forneceu 1-(4-tosil)piperidin-4-carboxilato de metila (1), que foi, em seguida, transformado em 1-(4-tosil)piperidin-4-carbo-hidrazida (2) por reação com hidrato de hidrazina em metanol. O composto 2 foi submetido a refluxo com CS2 na presença de KOH para se obter 5-(1-(4-tosil)piperidin-4-il)-1,3,4-oxadiazol-2-tiol (3). Os compostos desejados, 5a-h, foram obtidos por agitação de 3 com haletos de aralquila, 4a-h, em DMF, na presença de NaH. As estruturas dos compostos sintetizados foram elucidadas através de análise dos espectros de 1H-MNR, IR e EI-MS. Estes compostos foram, ainda, avaliados quanto à inibição das enzimas lipoxigenase e alfa-glucosidase, juntamente com a atividade antibacteriana contra bactérias Gram positivas e Gram negativas.


Assuntos
Antibacterianos/síntese química , Inibidores Enzimáticos/análise , Oxidiazóis/análise , Bactérias Gram-Negativas , Bactérias Gram-Positivas , Sulfonamidas/análise
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