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1.
Vaccimonitor (La Habana, Print) ; 28(3): 91-96, sept.-dic. 2019. graf
Artigo em Espanhol | LILACS | ID: biblio-1094630

RESUMO

RESUMEN En los procesos neuroinflamatorios se produce a nivel de líquido cefalorraquídeo una activación policlonal y poliespecífica. Esta activación se produce desde los primeros días y puede permanecer por períodos prolongados. Luego por mecanismos de apoptosis los clones que no responden directamente contra los agentes biológicos involucrados no proliferan. El Reibergrama permite saber si las inmunoglobulinas presentes en el líquido cefalorraquídeo se sintetizaron o no en el sistema nervioso central (SNC) y el Índice de Anticuerpo (IA) determina la especificidad de las mismas en caso de que exista síntesis intratecal. Con estas herramientas nos propusimos identificar la respuesta neuroinmunológica frente a agentes de la familia herpesvirus en pacientes pediátricos con proceso inflamatorio del SNC a partir de sus respectivos IA. Para lograr esto se cuantificaron los niveles de IgG y albúmina en suero y líquido cefalorraquídeo (LCR) mediante inmunodifusión radial simple y por ensayo inmunoenzimático, con lo cual se construyó el Reibergrama que permitió la selección de 85 pacientes pediátricos con síntesis intratecal de inmunoglobulinas, que se diferenciaron en cuatro grupos según sus edades. Mediante ensayo inmunoenzimático se cuantificaron los niveles de IgG específica contra citomegalovirus, virus varicela zoster y virus herpes simple, tanto en suero como en LCR y se determinó el IA específico. La respuesta contra los virus estudiados fue similar para los distintos grupos de edades, lo cual nos permite afirmar la exposición temprana a los mismos.


ABSTRACT In a neuroinflammatory process a polyclonal and poly-specific activation is produced in cerebrospinal fluid. This activation starts from the first days and may persist for a long time. The clones not related directly against the biological agent do not proliferate by apoptosis. Reibergram determine if part of the immunoglobulins content in cerebrospinal fluid belongs from the blood or it is synthesized in the central nervous system. Antibody index determines if the specific antibodies was synthesized intrathecally. By these tools it can be possible to identify the humoral immune response against some herpes virus in pediatric patients suffering from a central nervous system inflammatory process. Quantification of specific IgG against citomegalovirus, varicella zoster and herpes simplex virus in serum and cerebrospinal fluid was done by ELISA. Specific Antibody index against these viruses were similar for the different age groups, which confirm the early exposure of the population.


Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Líquido Cefalorraquidiano , Simplexvirus , Infecções por Citomegalovirus/diagnóstico , Infecção pelo Vírus da Varicela-Zoster/epidemiologia , Epidemiologia Descritiva , Estudos Transversais , Imunodifusão/métodos
6.
Rev. patol. trop ; 46(1): 01-21, abr. 2017. tab, ilus
Artigo em Inglês | LILACS | ID: biblio-913416

RESUMO

Over the past forty years, there has been a great advance in antiviral infections treatment. The discovery of acyclovir in 1977 paved the way to new antiviral drugs. Other nucleoside analogues such as valacyclovir, penciclovir, famciclovir, ganciclovir, valganciclovir, cidofovir and foscarnet were made available, as well as neuraminidase inhibitors. Also, drugs for the treatment of viral hepatitis and patients with HIV/AIDS have not only increased life quality and expectancy, but also decreased the incidence of some viral infections. Antiviral drugs are important tools to the clinician, especially when treating patients with impaired immunological and clinical condition. Aiming to restore health and prevent further adverse events, the clinician must be aware of the best antiviral drug available, its proper route of administration and dosage. The aim of this review is to present the antiviral drugs currently available, focusing on treatment of common viral infections in clinical practice. A brief description of the mechanisms of action and prescription of antiviral drugs is presented, using the data available from evidence-based medicine.


Assuntos
Antivirais , Orthomyxoviridae , Simplexvirus , Herpesvirus Humano 3 , Citomegalovirus
7.
Biomédica (Bogotá) ; 36(supl.2): 201-210, ago. 2016. graf, tab
Artigo em Espanhol | LILACS | ID: lil-794032

RESUMO

Introducción. El trasplante de precursores hematopoyéticos es una alternativa en el tratamiento de diversas condiciones en la población pediátrica. La intensidad del acondicionamiento para el trasplante predispone al desarrollo de complicaciones en los receptores. Las infecciones por el virus herpes simple 1 (HSV-1), el virus herpes simple 2 (HSV-2), el citomegalovirus (CMV) humano y el virus de Epstein-Barr (EBV) son una causa importante de morbimortalidad en estos pacientes. La reactivación de infecciones latentes puede producir descargas virales asintomáticas detectables en la saliva, lo cual ayuda a determinar el comportamiento de dichas infecciones en pacientes con trasplante y a establecer el diagnóstico temprano de la reactivación. Objetivo. Evaluar el comportamiento de la descarga viral de HSV-1, HSV-2, CMV y EBV en la saliva de pacientes hospitalizados en la Unidad de Trasplante de la Fundación HOMI - Hospital de la Misericordia, entre enero y noviembre de 2012. Materiales y métodos. Se evaluaron muestras de saliva de 17 receptores de trasplante. La presencia de ADN de HSV-1, HSV-2, CMV y EBV en las muestras de saliva se detectó mediante reacción en cadena de la polimerasa convencional. Resultados. Se detectó el ADN del HSV-2 en la saliva de cuatro pacientes, del CMV en la de cuatro y del EBV en la de nueve, lo cual se asoció con leucopenia. Cuatro de los 17 pacientes presentaron cargas simultáneas de CMV y EBV. No se detectó el ADN del HSV-1. Conclusiones: Se demostró una descarga asintomática de HSV-2, CMV y EBV asociada a leucopenia en la saliva de los pacientes.


Introduction: Hematopoietic stem cell transplantation in pediatric patients is an alternative treatment for different diseases. The conditioning regimen for transplant predisposes recipients to the development of infections. Viral infections by herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), human cytomegalovirus (CMV), and Epstein-Barr virus (EBV), are the most common, and the leading cause of morbidity and mortality among these patients. These viruses lie dormant in various cell types and the reactivation of latent infections may lead to asymptomatic viral shedding in saliva. The detection of these viruses in secretions may contribute to understand the behavioral dynamics of these viral infections in transplanted patients, and to the early diagnosis of reactivation. Objective: To assess HSV-1, HSV-2, CMV and EBV viral shedding in the saliva of patients admitted for hematopoietic stem cell transplantation at Fundación HOMI - Hospital de la Misericordia between January and November of 2012. Materials and methods: We evaluated stimulated saliva samples of 17 hematopoietic stem cell transplantation recipients weekly. We performed DNA extraction from saliva, and we evaluated the presence of DNA for HSV-1, HSV-2, CMV, and EBV by PCR. Results: While we detected HSV-2 and CMV DNA in the saliva of four patients, EBV DNA was detected in nine patients with leukopenia. In contrast, we did not detect HSV-1 DNA in saliva. Additionally, four out of the 17 patients showed a simultaneous shedding of CMV and EBV. Conclusions: By conventional PCR, we demonstrated asymptomatic HSV-2, CMV, and EBV viral shedding in saliva, associated with leukopenia.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Transplante de Medula Óssea , Citomegalovirus , Herpes Simples , Herpesviridae , Herpesvirus Humano 4 , Simplexvirus
8.
Rev. chil. infectol ; 32(1): 84-96, feb. 2015. ilus
Artigo em Espanhol | LILACS | ID: lil-742540

RESUMO

Herpes simplex viruses and humans have co-existed for tens of thousands of years. This long relationship has translated into the evolution and selection of viral determinants to evade the host immune response and reciprocally the evolution and selection of host immune components for limiting virus infection and damage. Currently there are no vaccines available to avoid infection with these viruses or therapies to cure them. Herpes simplex viruses are neurotropic and reside latently in neurons at the trigeminal and dorsal root ganglia, occasionally reactivating. Most viral recurrences are subclinical and thus, unnoticed. Here, we discuss the initial steps of infection by herpes simplex viruses and the molecular mechanisms they have developed to evade innate and adaptive immunity. A better understanding of the molecular mechanisms evolved by these viruses to evade host immunity should help us envision novel vaccine strategies and therapies that limit infection and dissemination.


Los virus herpes simplex y humanos co-existen desde decenas de miles de años. Esta prolongada relación se ha traducido en la evolución y selección de determinantes virales para evadir la respuesta inmune y recíprocamente la evolución y selección de componentes inmunes del hospedero para limitar la infección viral y el daño que producen. Actualmente no existen vacunas para evitar la infección de estos virus o terapias que la curen. Los virus herpes simplex son neurotrópicos y permanecen latentes en neuronas de ganglios trigémino y dorsales, reactivándose esporádicamente. La mayoría de las recurrencias por virus herpes simplex son sub-clínicas y por tanto pasan inadvertidas. Aquí discutimos los pasos iniciales de la infección porvirus herpes simplex y los mecanismos moleculares que estos virus han desarrollado para evadir la respuesta inmune innata y adaptativa. Una mejor comprensión de los mecanismos moleculares evolucionados por estos virus para evadir la respuesta inmune del hospedero deberían ayudarnos visualizar nuevas estrategias para desarrollar vacunas y terapias que limiten su infección y diseminación.


Assuntos
Humanos , Imunidade Adaptativa/imunologia , Herpes Simples/imunologia , Evasão da Resposta Imune , Simplexvirus/patogenicidade , Apoptose/fisiologia , Interferon Tipo I/imunologia , Simplexvirus/fisiologia , Latência Viral/fisiologia , Replicação Viral/fisiologia
9.
An. bras. dermatol ; 89(3): 448-452, May-Jun/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-711599

RESUMO

BACKGROUND: Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection. OBJECTIVE: This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine. METHODS: Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay. RESULTS: From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%. CONCLUSIONS: The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood. .


Assuntos
Humanos , Aciclovir/farmacologia , Antivirais/farmacologia , Foscarnet/farmacologia , Simplexvirus/efeitos dos fármacos , Simplexvirus/isolamento & purificação , Trifluridina/farmacologia , Células Cultivadas , Hematoxilina , Testes de Sensibilidade Microbiana , Fatores de Tempo , Ensaio de Placa Viral
10.
Dermatol. pediátr. latinoam. (En línea) ; 12(1): 15-20, ene.-abr. 2014. ilus
Artigo em Espanhol | LILACS | ID: lil-776147

RESUMO

Presentamos un caso de infección por Herpes virus simplex adquirido por vía transplacentaria en un neonato nacido a las 34 semanas de gestación por parto vaginal. La madre presentó un único episodio de infección genital por herpes virus en la décima semana de gestación.En el momento del nacimiento el niño presentódos ampollas y lesiones de aplasia cutis que semejaba una epidermólisis bullosa. Además, se evidenciaron múltiples anomalías congénitas del sistema nervioso central, oftalmológicas y cardíacas que sustentan la infección herpética intrauterina. El test de Tzanck y la serología para Herpes virus simplex 2 (IgG) fueron positivas. La infección intrauterina por virus herpes está asociada a alta morbilidad y mortalidad pero responde a la terapia antiviral. Este diagnóstico debe ser considerado en todo neonato con una enfermedad ampollar o piel erosionada.


We present a case of Herpes simplex virus infection acquired by transplacental route in a neonate born at 34 weeks of gestation by vaginal delivery. The mother presented a single episode of genital herpes simplex virus infection in the tenth week of pregnancy. At birth, the neonate presented two blisters and lesions of aplasia cutis resembling epidermolysis bullosa. In addition, he presented multiple congenital anomalies of the brain, eyes and heart, supporting the intrauterine infection. Tzanck test and serology for Herpes simplex virus 2(IgG) were positive. Intrauterine infection by herpes virus is associated with high morbidity and mortality, however it responds to antiviral therapy. This diagnosis should be considered in any neonate with blisters or denuded skin.


Assuntos
Humanos , Masculino , Recém-Nascido , Displasia Ectodérmica , Epidermólise Bolhosa , Herpes Genital , Herpes Simples , Simplexvirus , Simplexvirus/patogenicidade , Anormalidades Congênitas , Anormalidades da Pele
11.
Rev. Soc. Bras. Med. Trop ; 46(5): 625-628, Sept-Oct/2013. tab, graf
Artigo em Inglês | LILACS | ID: lil-691413

RESUMO

Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies. .


Assuntos
Humanos , DNA Viral/análise , Herpes Simples/diagnóstico , Herpes Zoster/diagnóstico , /genética , Reação em Cadeia da Polimerase , Simplexvirus/genética , Limite de Detecção , Sensibilidade e Especificidade
12.
Med. U.P.B ; 31(2): 181-192, jul.-dic. 2012.
Artigo em Espanhol | LILACS | ID: lil-689086

RESUMO

Objetivo: describir la frecuencia, y el comportamiento clínico y de laboratorio de las infecciones por citomegalovirus (CMV), virus Epstein-Barr (EBV) y virus herpes simplex 1 y 2 (HSV 1 y HSV 2) en el sistema nervioso central tanto en pacientes inmunocomprometidos como inmunocompetentes. Metodología: se analizaron 204 líquidos cefalorraquídeos con el uso de la reacción en cadena de la polimerasa (PCR, de su sigla en inglés) en tiempo real como herramienta diagnóstica molecular para la detección de infección. Resultados: un 16% de los líquidos cefalorraquídeos es positivo para alguno de los virus estudiados, el EBV se detectó en el 22.8% de los casos positivos y se ubica por encima de las infecciones ocasionadas por HSV y CMV. El 61.9% de los pacientes con resultado positivo tenía algún tipo de inmunocompromiso. onclusiones: la utilización de la PCR en tiempo real permite establecer el agente causal de infección en el sistema nervioso central y es de gran ayuda en los pacientes inmunocomprometidos en los que las variaciones clínicas y de laboratorio no son concluyentes. Esto permitiría la implementación de una terapia específica.


Assuntos
Humanos , Líquido Cefalorraquidiano , Citomegalovirus , Encefalite Viral , Infecções por Vírus Epstein-Barr , Meningite Asséptica , Reação em Cadeia da Polimerase , Simplexvirus
13.
Biomédica (Bogotá) ; 32(2): 233-238, abr.-jun. 2012. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-656832

RESUMO

Introducción. Pocos estudios han descrito la microbiota subgingival en mujeres embarazadas con preeclampsia leve. Objetivo. Identificar cambios periodontales y de la microbiota subgingival en mujeres embarazadas con preeclampsia, después del tratamiento periodontal. Materiales y métodos. En un análisis secundario de un ensayo clínico de asignación aleatoria, se estudiaron 57 pacientes con preeclampsia en el Hospital Universitario del Valle de Cali. Se asignaron al azar 31 al grupo de intervención periodontal (detartraje y alisado subgingival ultrasónico y manual) durante su embarazo y otras 26 al grupo control (profilaxis supragingival). Se determinaron los parámetros clínicos periodontales y la microbiota subgingival a la inclusión al estudio y en el posparto. Se evaluaron 8 bacterias periodontopáticas y 2 virus herpes por reacción en cadena de la polimerasa. Se usaron las pruebas de ji al cuadrado, test de McNemar o t de Student, con un nivel de significancia de p≤ Resultados. Los grupos fueron comparables en las variables clínicas y microbiológicas al inicio del estudio. El tratamiento periodontal redujo el promedio de la profundidad de bolsa en el grupo de intervención de 2,44±0,31 a 2,31±0,24 mm (p=0,000) y en el grupo control de 2,58±0,37 a 2,44±0,39 mm (p=0,000),y el índice de sangrado, de 16,4±1,5 a 7,9±0,7 % en el primero (p=0,000), y de 17,1±1,8 a 10±0,9 %, en el segundo (p=0,002). La frecuencia de detección de microorganismos no varió de manera significativa entre los grupos. Conclusión. El raspaje y alisado radicular, así como la profilaxis supragingival, redujeron de manera significativa la profundidad a la sonda y el índice de sangrado gingival. El tratamiento periodontal no fue más efectivo que la profilaxis para reducir los organismos periodontopáticos o los virus herpes.


Introduction. Few studies have described subgingival microbiota in pregnant women with mild preeclampsia. Objective. Clinical periodontal and subgingival microbiota changes were identified in pregnant women with mild preeclampsia after periodontal treatment. Materials and methods. In a secondary analysis of a randomized clinical trial, 57 preeclamptic women were studied at Hospital Universitario del Valle in Cali, Colombia. Thirty one women were randomized to the periodontal intervention group (subgingival scaling and planing ultrasonic and manual) during pregnancy and 26 to the control group (supragingival prophylaxis). Periodontal clinical parameters and subgingival microbiota were characterized at the time of acceptance into the study and again at postpartum. Eight periodontopathic bacteria and 2 herpesviruses were assessed by polymerase chain reaction. Chi-square, McNemar or Student´s t tests were used, with a significance level of p≤0.05. Results. Both groups were comparable in the clinical and microbiological variables at baseline. Periodontal treatment reduced the average pocket depth in the intervention group from 2.4±0.3 to 2.3±0.2 mm (p<0.001) and in control group 2.6±0.4 to 2.44±0.4 mm, (p<0.001) and bleeding index 16.4±1.5% to 7.9±0.7% in the intervention group(p<0.001) and 17.1±1.8% to 10±0.9% in the control group (p=0.002). The frequency of detection of microorganisms did not differ significantly between groups. Conclusion. Scaling/root planning and supragingival prophylaxis significantly reduced the probing depth and gingival bleeding index. Periodontal treatment was not more effective than prophylaxis in reducing periodontopathic organisms or herpesvirus.


Assuntos
Adulto , Feminino , Humanos , Gravidez , Raspagem Dentária , Metagenoma , Pré-Eclâmpsia/microbiologia , Aplainamento Radicular , Bactérias/isolamento & purificação , Polimento Dentário , Raspagem Dentária/métodos , Hemorragia Gengival/etiologia , Gengivite/complicações , Gengivite/microbiologia , Gengivite/prevenção & controle , Gengivite/terapia , Gengivite/virologia , /isolamento & purificação , Higiene Bucal , Educação de Pacientes como Assunto , Bolsa Periodontal/microbiologia , Bolsa Periodontal/prevenção & controle , Bolsa Periodontal/virologia , Periodontite/complicações , Periodontite/microbiologia , Periodontite/prevenção & controle , Periodontite/terapia , Periodontite/virologia , Complicações Infecciosas na Gravidez/microbiologia , Complicações Infecciosas na Gravidez/prevenção & controle , Complicações Infecciosas na Gravidez/terapia , Complicações Infecciosas na Gravidez/virologia , Transtornos Puerperais/microbiologia , Transtornos Puerperais/virologia , Aplainamento Radicular/métodos , Índice de Gravidade de Doença , Simplexvirus/isolamento & purificação
14.
Braz. j. infect. dis ; 15(3): 211-214, May-June 2011. tab
Artigo em Inglês | LILACS | ID: lil-589950

RESUMO

Diagnosis of herpes simplex encephalitis (HSE) is based on the detection of herpes simplex virus (HSV) DNA in patients' CSF samples. HSV DNA quantitation has the potential for estimating the effects of antiviral therapy. The aim of this study was to diagnose HSV DNA in HSE suspected patients and the quantitative analysis of its genome using real-time PCR to assess the value of the viral load in the course of antiviral treatment. The CSF samples were collected from 236 consecutive HSE suspected patients from November 2004 to May 2008. Upon DNA extraction, the samples were analyzed by Real-Time PCR assay. A set of primers amplified a common sequence of HSV glycoprotein B gene. The copy numbers of unknown samples were expressed via a standard curve drawn with a known amount of amplified cloned plasmid. Of the 236 samples, 137 (58 percent) came from males and 99 (42 percent) from females. The HSV genome was detected in 22 (9.3 percent) patients by PCR, 13 males/ 9 females. Serial CSF samples were available from 10 of the 22 patients. The range of the HSV DNA copy numbers in the clinical samples ranged from 2.5 × 10² to 1.7 × 10(6) copies/mL of CSF. Quantitative PCR results can be helpful in evaluating the efficacy of antiviral therapy in the above-mentioned patients. There is an association between the initial viral load and the duration of treatment course.


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , DNA Viral/líquido cefalorraquidiano , Encefalite por Herpes Simples/diagnóstico , Simplexvirus/genética , Aciclovir/uso terapêutico , Antivirais/uso terapêutico , DNA Viral/genética , Encefalite por Herpes Simples/tratamento farmacológico , Encefalite por Herpes Simples/virologia , Estudos Prospectivos , Reação em Cadeia da Polimerase/métodos , Simplexvirus/isolamento & purificação , Carga Viral
15.
Arq. neuropsiquiatr ; 69(3): 475-481, June 2011. ilus, tab
Artigo em Inglês | LILACS | ID: lil-592506

RESUMO

Viral meningitis is a common infectious disease of the central nervous system (CNS) that occurs worldwide. The aim of this study was to identify the etiologic agent of lymphomonocytary meningitis in Curitiba, PR, Brazil. During the period of July 2005 to December 2006, 460 cerebrospinal fluid (CSF) samples with lymphomonocytary meningitis were analyzed by PCR methodologies. Fifty nine (12.8 percent) samples were positive. Enteroviruses was present in 49 (83 percent) samples and herpes virus family in 10 (17 percent), of these 6 (10 percent) herpes simplex virus, 1 (2 percent) Epstein Barr virus, 2 (3 percent) human herpes virus type 6 and 1 (2 percent) mixed infection of enterovirus and Epstein Barr virus. As conclusion enterovirus was the most frequent virus, with circulation during summer and was observed with higher frequency between 4 to 17 years of age. PCR methodology is an important method for rapid detection of RNA enterovirus and DNA herpesvirus in CSF.


A meningite viral é uma síndrome infecciosa comum do sistema nervoso central (SNC), que ocorre no mundo inteiro. O objetivo deste estudo foi identificar o agente etiológico de meningite linfomonocitária em Curitiba, PR, Brasil. Durante o período de julho de 2005 a dezembro de 2006, 460 amostras com meningite linfomonocitária foram analisadas por metodologias de PCR. Cinquenta e nove (12,8 por cento) amostras foram positivas. Enterovirus estava presente em 49 (83 por cento) amostras e herpes vírus em 10 (17 por cento), destas 6 (10 por cento) HSV, 1 (2 por cento) EBV, 2 (3 por cento) HHV- 6 e 1 (2 por cento) infecção mista de enterovírus e EBV. Conclui-se que o enterovirus foi o vírus mais frequente, com a circulação durante o verão. Houve maior número de amostras positivas entre 4 a 17 anos. A metodologia de PCR é um importante método para a detecção rápida de RNA de enterovirus e DNA do herpesvirus no LCR.


Assuntos
Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Adulto Jovem , Infecções por Enterovirus/virologia , Enterovirus/genética , Infecções por Herpesviridae/virologia , Herpesviridae/genética , Meningite Viral/virologia , Brasil , DNA Viral/líquido cefalorraquidiano , Infecções por Enterovirus/diagnóstico , Infecções por Herpesviridae/diagnóstico , /genética , /genética , Meningite Viral/diagnóstico , Reação em Cadeia da Polimerase , RNA Viral/líquido cefalorraquidiano , Simplexvirus/genética
16.
Kasmera ; 39(1): 49-58, ene.-jun. 2011. tab
Artigo em Espanhol | LILACS | ID: lil-654001

RESUMO

Diversos virus afectan el sistema nervioso central (SNC) ocasionando encefalitis, principalmente en la edad pediátrica. Determinar la implicación de agentes virales en infecciones del sistema nervioso central (SNC) en niños del estado Zulia, Venezuela durante el año 2007. Se recolectaron 109 muestras de líquido cefalorraquídeo(LCR) y suero, provenientes de pacientes entre 1 día de nacido a 14 años, que presentaron sintomatología clínica sugestiva de afectación del SNC y cuyo estudio bacteriológico convencional de LCR resultó negativo. Se determinó la relación albúmina LCR/suero a fin de descartar contaminación, resultando 24 pares óptimos para la determinación por la técnica de ELISA de anticuerpos IgM específicos para los virus Herpes Simple (VHS), Epstein Barr (VEB), Dengue, Rubéola, Sarampión y Encefalitis Equina Venezolana (EEV). De los 24 casos analizados, 15 (62,5%) resultaron positivos. Los agentes causantes de encefalitis fueron: 11 casos de Dengue (45,8%) (p<0,05), 3 de VHS (12,5%) y un caso de VEB (4,2%). No se detectaron casos de Rubéola, Sarampión, ni EEV. La pleocitocis con predominio de linfocitos fue el hallazgo más frecuente en los casos con encefalitis viral (EV) confirmada, sin diferencias significativas al relacionarlo con el agente viral infectante. Se evidencia que una proporción significativa de los niños con encefalitis es debida a agentes virales y se destaca un incremento en los casos de dengue con afectación del SNC en la región


Various viruses affect the central nervous system (CNS) causing encephalitis, mainly in pediatric patients. To determine the involvement of viral agents for central nervous system (CNS) infections in children in the State of Zulia, Venezuela during the year 2007. 109 samples of cerebrospinal fluid (CSF) and serum were collected from patients between 1 day and 14 years of age, who presented clinical symptoms suggestive of CNS involvement and whose conventional CSF bacteriological study proved negative. The CSF Albumin /serum relation ship was determined in order to rule out contamination, resulting in 24 optimal pairs for determining the IgM and IgG antibodies specific for herpes simplex virus (HSV), Epstein Barr (EBV), Dengue fever, rubella, measles and Venezuelan Equine Encephalitis (VEE) using the ELISA technique. Of the 24 cases examined, 15 (62.5%) were positive. The causative agents for encephalitis were 11 cases of Dengue (45.8%) (p<0.05), 3 VHS (12.5%) and 1 case of EBV (4.2%). There were no cases of rubella, measles or VEE. Pleocytosis with lymphocyte predominance was the most common finding in cases with confirmed viral encephalitis (VE), without significant differences related to the infecting viral agent. Results show that a significant proportion of encephalitis in children is due to viral agents, highlighting an increase in dengue cases with CNS affection in the region


Assuntos
Humanos , Masculino , Adolescente , Feminino , Recém-Nascido , Lactente , Pré-Escolar , Criança , Dengue/patologia , Encefalite/patologia , Líquido Cefalorraquidiano/virologia , Simplexvirus , Sarampo/patologia , Sistema Nervoso Central/virologia , Ensaio de Imunoadsorção Enzimática/métodos
17.
Braz. j. med. biol. res ; 44(5): 421-427, May 2011. ilus
Artigo em Inglês | LILACS | ID: lil-586516

RESUMO

Anti-cancer DNA vaccines have attracted growing interest as a simple and non-invasive method for both the treatment and prevention of tumors induced by human papillomaviruses. Nonetheless, the low immunogenicity of parenterally administered vaccines, particularly regarding the activation of cytotoxic CD8+ T cell responses, suggests that further improvements in both vaccine composition and administration routes are still required. In the present study, we report the immune responses and anti-tumor effects of a DNA vaccine (pgD-E7E6E5) expressing three proteins (E7, E6, and E5) of the human papillomavirus type 16 genetically fused to the glycoprotein D of the human herpes simplex virus type 1, which was administered to mice by the intradermal (id) route using a gene gun. A single id dose of pgD-E7E6E5 (2 µg/dose) induced a strong activation of E7-specific interferon-γ (INF-γ)-producing CD8+ T cells and full prophylactic anti-tumor effects in the vaccinated mice. Three vaccine doses inhibited tumor growth in 70 percent of the mice with established tumors. In addition, a single vaccine dose consisting of the co-administration of pgD-E7E6E5 and the vector encoding interleukin-12 or granulocyte-macrophage colony-stimulating factor further enhanced the therapeutic anti-tumor effects and conferred protection to 60 and 50 percent of the vaccinated mice, respectively. In conclusion, id administration of pgD-E7E6E5 significantly enhanced the immunogenicity and anti-tumor effects of the DNA vaccine, representing a promising administration route for future clinical trials.


Assuntos
Animais , Feminino , Camundongos , Vacinas Anticâncer/administração & dosagem , /imunologia , Proteínas Oncogênicas Virais/imunologia , Simplexvirus/imunologia , Vacinas de DNA/administração & dosagem , Proteínas do Envelope Viral/imunologia , /imunologia , Vacinas Anticâncer/genética , Vacinas Anticâncer/imunologia , /genética , Injeções Intradérmicas , Neoplasias Experimentais/imunologia , Neoplasias Experimentais/prevenção & controle , Proteínas Oncogênicas Virais/genética , Simplexvirus/genética , Vacinas de DNA/genética , Vacinas de DNA/imunologia , Proteínas do Envelope Viral/genética
18.
Rev. estomat. salud ; 19(1): 26-29, 2011.
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-868184

RESUMO

La periodontitis es una enfermedad infec-ciosa caracterizada por un conjunto de alte-raciones que afectan los tejidos soporte de las piezas dentales. Su etiología por muchos años ha sido considerada básicamente bac-teriana. Estudios recientes han analizado la presencia de algunos miembros de la fami-lia de los virus de la familia herpesviridae en el periodonto y el posible efecto que su infección podría ocasionar en él. Esta presentación de un caso clínico contribuye a la evidencia que vincula a algunos de los herpes virus en la etiopatogénesis de la enfermedad periodontal...(AU)


Periodontitis is an infectious disease cha-racterized by a variety of alterations, affec-ting tooth supporting tissues. For many years, its aetiology has been considered basically of bacterial origin. Recent studies have analyzed the occurrence of herpes vi-ruses in the periodontum and their possible effects to cause periodontitis. This case report provides evidence that link herpes viruses to the development of certain types of periodontitis...(AU)


Assuntos
Humanos , Medicina Bucal , Doenças Periodontais , Periodontia , Simplexvirus , Periodontite Crônica , Odontologia , Herpes Zoster , Periodontia/classificação
19.
Rev. méd. Chile ; 138(10): 1302-1311, oct. 2010. ilus
Artigo em Inglês | LILACS | ID: lil-572945

RESUMO

The classic hepatotropic viruses, hepatitis A through E, are not the only viral agents able to infect the liver. Other systemic viruses may cause hepatic injury that can range from mild and transient elevation of aminotransferases to acute hepatitis and occasionally acute liver failure and fulminant hepatitis. The clinical presentation may be indistinguishable from that associated with classic hepatotropic viruses. These agents include cytomegalovirus; Epstein-Barr virus; herpes simplex virus; varicella-zoster virus; human herpesvirus 6, 7, and 8; human parvovirus B19; adenoviruses among others. Wide spectrums of clinical syndromes are associated with cytomegalovirus disease. Unique clinical syndromes may present in neonates, young adults and immunocompromised hosts infected with cytomegalovirus. Cases of fulminant hepatitis have been reported in both immunocompromised and immunocompetent hosts infected with Epstein Barr virus. Occasionally, these patients with acute hepatic failure may need liver transplantation. Herpes simplex viruses may involve the liver in neonatal infections, pregnancy, immunocompromised hosts and occasionally, immunocompetent adults. Varicella-Zoster virus has also been associated with severe acute hepatitis and fulminant hepatitis in adults. The drug of choice for these conditions is intravenous acyclovir. These may also need liver transplantation in the more severe forms of clinical presentation. Typical liver biopsy findings can be useful in determining the diagnosis of these viral infections. Human herpesviruses 6, 7, and 8, human parvovirus B19, and adenoviruses can also be present with features of acute liver injury and occasionally as fulminant hepatitis. The clinical syndromes are less well delineated than those associated with herpesviruses. It is important to consider these viruses as possible etiologic agents in patients who have acute liver injury and their serologic markers for the classic hepatotropic viruses are not indicative of an active infection.


Los agentes de la hepatitis viral A, B, C, D y E no son los únicos virus que pueden causar un síndrome de daño hepático agudo. Agentes virales como el citomegalovirus, Epstein-Barr, herpes simplex 1 y 2, Varicella-Zoster, virus herpes humano 6, 7, y 8, parvovirus B19 y adenovirus pueden causar daño hepático agudo e inclusive presentarse como hepatitis fulminante. Los cuadros clínicos de daño hepático agudo por citomegalovirus, Epstein Barr y herpes simplex 1 y 2 han sido caracterizado mejor. Se ha intentado el uso de drogas antivirales específicas como el uso intravenoso de aciclovir. Ocasionalmente, se ha requerido el trasplante hepático para rescatar pacientes con hepatitis fulminantes por estos agentes virales. La biopsia hepática puede ser de utilidad en estos casos puesto que los hallazgos son bastante característicos. La expresión clínica asociada a infecciones por virus herpes humano 6, 7 y 8, parvovirus B19 y adenovirus son menos características. Ha habido varios casos de hepatitis fulminante causada por estos agentes virales. Estos agentes virales deben ser considerados en el diagnóstico de casos de daño hepático agudo e inclusive hepatitis fulminante cuando los marcadores virales para los virus de hepatitis A-E son negativos.


Assuntos
Humanos , Hepatite Viral Humana/virologia , Fígado/virologia , Citomegalovirus/patogenicidade , /patogenicidade , /patogenicidade , /patogenicidade , Simplexvirus/patogenicidade
20.
Rev. chil. infectol ; 27(1): 60-64, feb. 2010.
Artigo em Espanhol | LILACS | ID: lil-537173

RESUMO

Sexually transmitted diseases are highly prevalent and a public health concern worldwide. Herpes simplex virus (HSV) and human papilloma virus (HPV) are described. The development of rapid, sensible and specific diagnostic assays has been difficult because of their pathogenic features. In the last years, molecular biology based techniques allowed a better and wider range of diagnosis, as in the HPV-cancer association. In this article, standardized diagnostic methodologies for HSV and HPV are reviewed.


Las Infecciones virales de transmisión sexual son altamente prevalentes y constituyen un problema de salud pública en el mundo. Entre los agentes que se contagian por esta vía, se describe acerca de virus herpes simplex (HSV) y virus papiloma humano (HPV). Las características patogénicas de estas infecciones han dificultado la implementación de técnicas diagnósticas rápidas, sensibles y específicas para el diagnóstico clínico habitual. En los últimos años las metodologías diagnósticas sustentadas en la biología molecular han permitido mej orar y ampliar el rango de diagnóstico posible para estos agentes infecciosos y relacionarlos con otras patologías, como es el caso de HPV y cáncer. En el presente artículo se revisan metodologías diagnósticas implementadas para el diagnóstico microbiológico de HSV y de HPV.


Assuntos
Feminino , Humanos , Masculino , Herpes Simples/diagnóstico , Papillomaviridae , Infecções por Papillomavirus/diagnóstico , Simplexvirus , Doenças Sexualmente Transmissíveis/diagnóstico , Doenças Sexualmente Transmissíveis/virologia , Herpes Simples/transmissão , Papillomaviridae/genética , Papillomaviridae/imunologia , Infecções por Papillomavirus/transmissão , Simplexvirus/genética , Simplexvirus/imunologia
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