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Braz. j. med. biol. res ; 50(12): e6351, 2017. graf
Artigo em Inglês | LILACS | ID: biblio-888959


Lippia sidoides Cham is a typical herb species of Northeast Brazil with widespread use in folk medicine. The major constituents of the essential oil of L. sidoides (EOLs) are thymol, p-cymene, myrcene, and caryophyllene. Several studies have shown that the EOLs and its constituents have pharmacological effects, including antibacterial, anti-inflammatory, antioxidant and neuroprotective activity. Therefore, this work aimed to investigate the effects of the EOLs and their main constituents on rat sciatic nerve excitability. The sciatic nerves of adult Wistar rats were dissected and mounted in a moist chamber. Nerves were stimulated by square wave pulses, with an amplitude of 40 V, duration of 100 μs to 0.2 Hz. Both EOLs and thymol inhibited compound action potential (CAP) in a concentration-dependent manner. Half maximal inhibitory concentration for CAP peak-to-peak amplitude blockade were 67.85 and 40 µg/mL for EOLs and thymol, respectively. CAP peak-to-peak amplitude was significantly reduced by concentrations ≥60 µg/mL for EOLs and ≥30 µg/mL for thymol. EOLs and thymol in the concentration of 60 µg/mL significantly increased chronaxie and rheobase. The conduction velocities of 1st and 2nd CAP components were also concentration-dependently reduced by EOLs and thymol in the range of 30-100 µg/mL. Differently from EOLs and thymol, p-cymene, myrcene and caryophyllene did not reduce CAP in the higher concentrations of 10 mM. These data demonstrated that EOLs and thymol inhibited neuronal excitability and were promising agents for the development of new drugs for therapeutic use.

Animais , Masculino , Feminino , Alcenos/farmacologia , Lippia/química , Monoterpenos/farmacologia , Óleos Voláteis/farmacologia , Nervo Isquiático/efeitos dos fármacos , Sesquiterpenos/farmacologia , Timol/farmacologia , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Bloqueio Nervoso/métodos , Condução Nervosa/efeitos dos fármacos , Óleos Voláteis/química , Ratos Wistar , Reprodutibilidade dos Testes , Nervo Isquiático/fisiologia , Fatores de Tempo
J. appl. oral sci ; 23(2): 145-152, Mar-Apr/2015. graf
Artigo em Inglês | LILACS | ID: lil-746536


Periodontal ligament mesenchymal stem cells (PDLMSCs) are an important alternative source of adult stem cells and may be applied for periodontal tissue regeneration, neuroregenerative medicine, and heart valve tissue engineering. However, little is known about the impact of bacterial toxins on the biological properties of PDLSMSCs, including self-renewal, differentiation, and synthesis of extracellular matrix. Objective : This study investigated whether proliferation, expression of pro-inflammatory cytokines, and osteogenic differentiation of CD105-enriched PDL progenitor cell populations (PDL-CD105+ cells) would be affected by exposure to bacterial lipopolysaccharide from Escherichia coli (EcLPS). Material and Methods : Toll-like receptor 4 (TLR4) expression was assessed in PDL-CD105+ cells by the immunostaining technique and confirmed using Western blotting assay. Afterwards, these cells were exposed to EcLPS, and the following assays were carried out: (i) cell viability using MTS; (ii) expression of the interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor alpha (TNF-α) genes; (iii) osteoblast differentiation assessed by mineralization in vitro, and by mRNA levels of run-related transcription factor-2 (RUNX2), alkaline phosphatase (ALP) and osteocalcin (OCN) determined by quantitative PCR. Results : PDL-CD105+ cells were identified as positive for TLR4. EcLPS did not affect cell viability, but induced a significant increase of transcripts for IL-6 and IL-8. Under osteogenic condition, PDL-CD105+ cells exposed to EcLPS presented an increase of mineralized matrix deposition and higher RUNX2 and ALP mRNA levels when compared to the control group. Conclusions : These results provide evidence that CD105-enriched PDL progenitor cells are able to adapt to continuous Escherichia coli endotoxin challenge, leading to an upregulation of osteogenic activities. .

Alcenos/metabolismo , /química , Staphylococcaceae/enzimologia , Catálise , Estabilidade Enzimática , Microbiologia Industrial , Concentração Osmolar
Rev. salud pública ; 10(1): 113-125, ene.-feb. 2008. tab
Artigo em Espanhol | LILACS | ID: lil-479057


Objetivo: Determinar el perfil de salud de los trabajadores de una Planta procesadora de Olefinas del Estado Zulia. Métodos: Se realizó un estudio observacional descriptivo, evaluando a 142 trabajadores de las áreas administrativa- técnica, operaciones y mantenimiento. A cada trabajador se le realizó una historia médico ocupacional, examen físico, exámenes de laboratorio, audiometría y evaluación visual. El grado de obesidad se determinó mediante la clasificación de la OMS, el riesgo cardiovascular se estableció aplicando las tablas de riesgo de las Sociedades Europeas. Resultados: El promedio de edad de los trabajadores fue de 36,2 ± 6,9 años. Los individuos del área de operaciones mostraron promedio de peso, y tensión arterial sistólica más elevada que el resto de las áreas. Se determinó una alta frecuencia de factores de riesgo cardiovascular predominando el consumo de alcohol (89,4 por ciento), obesidad (62,7 por ciento), hipertrigliceridemia (46,5 por ciento), y presión arterial sistólica elevada (45,1 por ciento); siendo los operadores los más afectados. Se encontró una frecuencia moderada de trabajadores con enzimas hepáticas alteradas AST (38 por ciento) y GGT (29,6 por ciento). Las patologías más frecuentes fueron ametropia (64,1 por ciento), dislipidemia (57,8 por ciento), obesidad I (50 por ciento), e hipertensión (45,1 por ciento). Los mayores de 40 años mostraron cifras de tensión arterial sistólica más elevadas. Se encontró una correlación positiva y significativa entre IMC y tensión sistólica. Conclusión: Existe una alta prevalencia de factores de riesgo cardiovascular. Se recomienda diseñar y aplicar acciones de promoción de la salud, encaminadas a modificar los estilos de vida para disminuir el riesgo de enfermedad cardiovascular.

Objective: Determining the profile of health for people working in an olefin plant. Methods: This was an observational, descriptive study carried out on 142 workers from technical-administrative, operator and maintenance areas. Each worker's employment and medical history was examined; they had a physical examination and laboratory, audiometric and visual tests. The degree of obesity was determined by WHO classification; cardiovascular risk was classified by using the European Societies' risk table. Results: The workers had a mean age of 36,2±6,9. The operators presented mean weight and systolic pressure which was higher than that of the rest of the workers. A high risk factor frequency was determined for cardiovascular pathologies: alcohol consumption (89,4 percent), obesity (62,7 percent), hypertriglycerides in the blood (46,5 percent) and high systolic pressure (45,1 percent); operators were the most affected workers. A moderate frequency of workers having altered hepatic enzymes, AST (38 percent) and GGT (29,6 percent) were observed The most frequently encountered pathologies were ametropia (64,1 percent), dyslipidaemia (57,8 percent), obesity (50 percent) and hypertension (45,1 percent). Workers aged more than 40 presented higher systolic pressure. A significant correlation between IMC and systolic pressure was found. Conclusion A high prevalence of cardiovascular risk factors was found. Health-care should be designed and promoted and action should be taken towards modifying lifestyles, aimed at reducing the risk of cardiovascular disease.

Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Alcenos , Exposição Ocupacional , Saúde do Trabalhador , Venezuela